CN102669663B - Method for preparing active calcium powder by using teleostean chine - Google Patents
Method for preparing active calcium powder by using teleostean chine Download PDFInfo
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Abstract
The invention relates to a method for preparing active calcium powder by using teleostean chine, belonging to the technical field of preparation of active calcium by using animal-based raw materials. The method comprises the following steps: with teleostean chine as raw material, degreasing by lipase hydrolysis, deodorizing with natural medicinal plants, crushing, then hydrolyzing proteins in the teleostean chine powder under the catalysis of mixed enzymes and finally carrying out spray drying to obtain the active calcium powder. According to the method, proteins in the teleostean chine powder are hydrolyzed by using enzymes to obtain calcium ions, a large part of calcium ions exist in a form of calcium amino acid and a small part of calcium ions exist in a form of calcium hydrophosphate, and another small part of calcium ions exist in a form of calcium phosphate precipitate; therefore, the active calcium powder product is rich in calcium amino acid and phosphorus, is a natural biologically active calcium powder with high quality and high grade.
Description
Technical field
The present invention relates to a kind ofly utilize teleostean fish spine to produce the method for activated calcium powder, belong to the technical field that animal derived raw material is produced calcium activated.
Background technology
The existing product of replenishing the calcium often is divided into organic calcium and inorganic calcium, and inorganic calcium low price, assimilation effect are poor, and as calcium carbonate, calcium bicarbonate, the organic calcium price is more expensive, absorbs and will get well relatively, as calcium gluconae, calcium citrate etc.But these all come from chemical preparation, belong to synthetic calcium.The American National dairy products committee thinks that the calcium in the food absorbs easily than synthetic calcium, there are oyster shell, dried scallop shell, coral, eggshell, ox bone, whey, fish-bone etc. in most popular natural calcium source, oyster shell has very likely been accumulated a large amount of heavy metals because being subjected to seawater pollution, and it is the same with eggshell, dried scallop shell, coral etc., after burning till processing, become calcium carbonate, the food dealer does not generally accept calcium carbonate, and this kind is calcareous that a strand excitant taste arranged.Bovine bone powder since BSE popular after, also give up need not more at present.As for whey calcium, calcium lactate, calcium gluconae etc. because being water-soluble, so calcium content is low.Fish-bone after treatment, the calcium content height, impurity is few, and very approaching with the calcareous component of skeleton, so fish-bone calcium has become the main flow in natural calcium source at present in advanced country.Application number is 00126212.2 Chinese invention patent, discloses a kind of effective treating method of eel bone, comprises the steps: that 1. eel bone being added water hots plate, and separates to cook liquid and bone; 2. add water and endopeptidase albuminoid enzyme in the bone after hotting plate separation, hydrolysis is attached to the protein on the bone, isolated protein hydrolyzate and bone; 3. add water and lipase in isolated bone from protein hydrolyzate, residual fat in the hydrolysis bone is removed this fat splitting liquid; 4. will remove key dry, the pulverizing of fat splitting liquid.This patent provides one well to extract calcareous method from animal skeleton.But with product, its fishy smell is laid particular stress on, and has caused the part consumer to be difficult to accept.
Summary of the invention
The objective of the invention is for solving the problems of the technologies described above, provide a kind of and utilize teleostean fish spine to produce the method for activated calcium powder.
The objective of the invention is to be achieved by the following technical programs:
Utilize teleostean fish spine to produce the method for activated calcium powder, may further comprise the steps:
1. put into reaction vessel after bony fish fish spine being cleaned, lipase is added water to submerge, and transferring pH is 7.4～7.8, is warming up to 45～55 ℃, reacts;
2. the fish spine is taken out, crushed after being dried becomes particle diameter at the coarse granule of 2～5mm, bind up with gauze after the raw meat agent evenly mixes with the special weight ratio except raw meat agent=1 ︰ 0.5～2 of fish spine Cu Ke Li ︰ with special removing then, place water to boil 1～2 hour, discard water, get coarse granule and the special mixture except the raw meat agent of fish spine, sieve, remove special for the raw meat agent;
3. with dry after the coarse granule rinsing of fish spine, be ground into the following particulate of particle diameter 1mm, use the mixed enzyme hydrolysis then, filter reserved filtrate after the hydrolysis;
4. described filtrate is made powder by spray-dired mode, namely get activated calcium powder;
Described special except the raw meat agent be to be the powdered substance that main component is made with the Chinese medicine root of Dahurain angelica, orange peel, Radix Glycyrrhizae.
Prior art directly is ground into powder with fish-bone after normally impurity such as the lipid in the fish-bone, polysaccharide being removed, be rich in not high calcareous of ossein, protein, polypeptide and content in this product, therefore this product is fit to cook health products, rather than the special product of replenishing the calcium, this added value of product is relatively low.The present invention is raw material with the fish spine, adopts the lipase hydrolysis degreasing, utilizes botanical herbs to take off raw meat, again to the mixed enzyme catalyzing hydrolysis of protein in the fishbone dust, forms finally by spray-drying after the pulverizing.The present invention passes through the protein digestion in the fish spine, thereby hydrolysis goes out calcium ion from the fish spine, and the calcium ion major part exists with the amino acid calcium form, and small part exists with the formation of calcium monohydrogen phosphate, also has small part to form calcium phosphate precipitation.Therefore be rich in amino acid calcium and phosphorus in the product, be natural biologically active fish-bone calcium powder, good product quality class height.Moreover, the present invention also utilizes Chinese medicine to remove the fishy smell in the fish spine, solved the problem of product fishy smell, has solved part consumer does not accept the fish-bone calcium powder because of fishlike smell problem.In the daily life, people's yellow rice wine commonly used and Jiang Lai removing fishy odor.About the unified explanation of the present neither one of fishlike smell, main flow scholar thinks that the existence of fishlike smell mainly is because of a kind of material that is trimethylamine, but trimethylamine content height in the seawater fish, content is few in fresh-water fishes; Also have the scholar to propose, fish has fishy smell to be because its side line, and lateral-line system is exactly the nervous system of fish, and common people often claim that side line is the raw meat line usually.The scholar thinks at smell, the fishy smell of fish is ubiquitous, fishy smell difference between the different fishes, fishlike smell is a kind of material that information is transmitted in mutual identification especially between the fish, as long as the release of this material of a part is arranged, similar fish just can be experienced, and usually strong fishlike smell transmission be a kind of danger signal, this taste is fishy smell In the view of we mankind, In the view of them is not; Usually among the people earlier fish being hit when killing fish dizzyly killed again, can significantly reduce fishlike smell.The inventor has proposed to go with Chinese medicine the theory of raw meat after having studied all kinds of theories, majoring in the Chinese herbal medicine works and looking for prescription folk prescription among the people, and final clear and definite to be with the root of Dahurain angelica, orange peel, Radix Glycyrrhizae that main component is made special for the raw meat agent.
Preferred, described special for also having tea leaf powder in the raw meat agent as technique scheme.
The adding of tea leaf powder, make described special except the raw meat agent except the raw meat better effects if.
Preferred as technique scheme, described mixed enzyme are the mixing enzyme preparations that alkali protease, neutral proteinase, papain, trypsase are made with the weight ratio of 1 ︰, 1 ︰, 1 ︰ 1.
Preferred as technique scheme, the consumption of described mixing enzyme preparation is 5～10 g/kg.
Preferred as technique scheme, the consumption of described lipase is 1～5 g/kg.
Preferred as technique scheme, described special weight ratio except the root of Dahurain angelica, orange peel, Radix Glycyrrhizae in the raw meat agent is 2 ︰, 2 ︰ 1.
Another kind as technique scheme is preferred, and described special weight ratio except the root of Dahurain angelica, orange peel, Radix Glycyrrhizae in the raw meat agent is 2 ︰, 1 ︰ 1.
The present invention has following technique effect:
Be rich in amino acid calcium and phosphorus in the product that the present invention makes, the calcium content height is natural biologically active fish-bone calcium powder, and human body easily absorbs, good product quality, added value height.And, except the raw meat technology fishy smell in the fish-bone is removed through unique, overcome a minority does not accept the fish-bone calcium powder because of the fish raw meat problem.
The specific embodiment
This specific embodiment only is explanation of the invention; it is not limitation of the present invention; those skilled in the art can make any modification to present embodiment as required after reading this specification, but as long as all are subjected to the protection of Patent Law in claim scope of the present invention.
Embodiment one
Utilize teleostean fish spine to produce the method for activated calcium powder, may further comprise the steps:
1. put into reaction vessel after bony fish fish spine being cleaned, lipase is added water to submerge, and transferring pH is 7.4, is warming up to 45 ℃, reacts;
2. the fish spine is taken out, crushed after being dried becomes particle diameter at the coarse granule of 2mm, bind up with gauze after the raw meat agent evenly mixes with the special weight ratio except raw meat agent=1 ︰ 0.5 of fish spine Cu Ke Li ︰ with special removing then, place water to boil 1 hour, discard water, get coarse granule and the special mixture except the raw meat agent of fish spine, sieve, remove special for the raw meat agent;
3. with dry after the coarse granule rinsing of fish spine, be ground into the following particulate of particle diameter 1mm, use the mixed enzyme hydrolysis then, filter reserved filtrate after the hydrolysis;
4. described filtrate is made powder by spray-dired mode, namely get activated calcium powder;
Described special except the raw meat agent be to be the powdered substance that main component is made with the Chinese medicine root of Dahurain angelica, orange peel, Radix Glycyrrhizae; Described special weight ratio except the root of Dahurain angelica, orange peel, Radix Glycyrrhizae in the raw meat agent is 1 ︰, 1 ︰ 1.
Described mixed enzyme is the mixing enzyme preparation that alkali protease, papain and trypsase are made with the weight ratio of 1 ︰, 1 ︰ 2.The consumption of described mixing enzyme preparation is 5g/kg.The consumption of described lipase is 1g/kg.
Embodiment two
Utilize teleostean fish spine to produce the method for activated calcium powder, may further comprise the steps:
1. put into reaction vessel after bony fish fish spine being cleaned, lipase is added water to submerge, and transferring pH is 7.5, is warming up to 46 ℃, reacts;
2. the fish spine is taken out, crushed after being dried becomes particle diameter at the coarse granule of 3mm, bind up with gauze after the raw meat agent evenly mixes with the special weight ratio except raw meat agent=1 ︰ 0.8 of fish spine Cu Ke Li ︰ with special removing then, place water to boil 1 hour, discard water, get coarse granule and the special mixture except the raw meat agent of fish spine, sieve, remove special for the raw meat agent;
3. with dry after the coarse granule rinsing of fish spine, be ground into the following particulate of particle diameter 1mm, use the mixed enzyme hydrolysis then, filter reserved filtrate after the hydrolysis;
4. described filtrate is made powder by spray-dired mode, namely get activated calcium powder;
Described special except the raw meat agent be to be the powdered substance that main component is made with the Chinese medicine root of Dahurain angelica, orange peel, Radix Glycyrrhizae; Described special weight ratio except the root of Dahurain angelica, orange peel, Radix Glycyrrhizae in the raw meat agent is 1 ︰, 2 ︰ 1,
Described special removing in the raw meat agent also contained tea leaf powder.The addition of tea leaf powder can be 10%～50% of the root of Dahurain angelica, orange peel and Radix Glycyrrhizae gross mass.
Described mixed enzyme is the mixing enzyme preparation that alkali protease, papain and trypsase are made with the weight ratio of 1 ︰, 2 ︰ 2.The consumption of described mixing enzyme preparation is 6g/kg.The consumption of described lipase is 2g/kg.
Embodiment three
Utilize teleostean fish spine to produce the method for activated calcium powder, may further comprise the steps:
1. put into reaction vessel after bony fish fish spine being cleaned, lipase is added water to submerge, and transferring pH is 7.6, is warming up to 48 ℃, reacts;
2. the fish spine is taken out, crushed after being dried becomes particle diameter at the coarse granule of 3mm, bind up with gauze after the raw meat agent evenly mixes with the special weight ratio except raw meat agent=1 ︰ 1 of fish spine Cu Ke Li ︰ with special removing then, place water to boil 1.5 hours, discard water, get coarse granule and the special mixture except the raw meat agent of fish spine, sieve, remove special for the raw meat agent;
3. with dry after the coarse granule rinsing of fish spine, be ground into the following particulate of particle diameter 1mm, use the mixed enzyme hydrolysis then, filter reserved filtrate after the hydrolysis;
4. described filtrate is made powder by spray-dired mode, namely get activated calcium powder;
Described special except the raw meat agent be to be the powdered substance that main component is made with the Chinese medicine root of Dahurain angelica, orange peel, Radix Glycyrrhizae; Described special weight ratio except the root of Dahurain angelica, orange peel, Radix Glycyrrhizae in the raw meat agent is 2 ︰, 2 ︰ 1,
Described special removing in the raw meat agent also contained tea leaf powder.The addition of tea leaf powder can be 10%～50% of the root of Dahurain angelica, orange peel and Radix Glycyrrhizae gross mass.
Described mixed enzyme is the mixing enzyme preparation that alkali protease, neutral proteinase, papain and trypsase are made with the weight ratio of 1 ︰, 1 ︰, 1 ︰ 2.The consumption of described mixing enzyme preparation is 7g/kg.The consumption of described lipase is 3g/kg.
Embodiment four
Utilize teleostean fish spine to produce the method for activated calcium powder, may further comprise the steps:
1. put into reaction vessel after bony fish fish spine being cleaned, lipase is added water to submerge, and transferring pH is 7.8, is warming up to 50 ℃, reacts;
2. the fish spine is taken out, crushed after being dried becomes particle diameter at the coarse granule of 3mm, bind up with gauze after the raw meat agent evenly mixes with the special weight ratio except raw meat agent=1 ︰ 1.5 of fish spine Cu Ke Li ︰ with special removing then, place water to boil 2 hours, discard water, get coarse granule and the special mixture except the raw meat agent of fish spine, sieve, remove special for the raw meat agent;
3. with dry after the coarse granule rinsing of fish spine, be ground into the following particulate of particle diameter 1mm, use the mixed enzyme hydrolysis then, filter reserved filtrate after the hydrolysis;
4. described filtrate is made powder by spray-dired mode, namely get activated calcium powder;
Described special except the raw meat agent be to be the powdered substance that main component is made with the Chinese medicine root of Dahurain angelica, orange peel, Radix Glycyrrhizae; Described special weight ratio except the root of Dahurain angelica, orange peel, Radix Glycyrrhizae in the raw meat agent is 2 ︰, 1 ︰ 1,
Described special removing in the raw meat agent also contained tea leaf powder.The addition of tea leaf powder can be 10%～50% of the root of Dahurain angelica, orange peel and Radix Glycyrrhizae gross mass.
Described mixed enzyme is the mixing enzyme preparation that alkali protease, neutral proteinase, papain and trypsase are made with the weight ratio of 1 ︰, 1 ︰, 1 ︰ 1.The consumption of described mixing enzyme preparation is 8g/kg.The consumption of described lipase is 4g/kg.
Embodiment five
Utilize teleostean fish spine to produce the method for activated calcium powder, may further comprise the steps:
1. put into reaction vessel after bony fish fish spine being cleaned, lipase is added water to submerge, and transferring pH is 7.8, is warming up to 55 ℃, reacts;
2. the fish spine is taken out, crushed after being dried becomes particle diameter at the coarse granule of 5mm, bind up with gauze after the raw meat agent evenly mixes with the special weight ratio except raw meat agent=1 ︰ 2 of fish spine Cu Ke Li ︰ with special removing then, place water to boil 2 hours, discard water, get coarse granule and the special mixture except the raw meat agent of fish spine, sieve, remove special for the raw meat agent;
3. with dry after the coarse granule rinsing of fish spine, be ground into the following particulate of particle diameter 1mm, use the mixed enzyme hydrolysis then, filter reserved filtrate after the hydrolysis;
4. described filtrate is made powder by spray-dired mode, namely get activated calcium powder;
Described special except the raw meat agent be to be the powdered substance that main component is made with the Chinese medicine root of Dahurain angelica, orange peel, Radix Glycyrrhizae; Described special weight ratio except the root of Dahurain angelica, orange peel, Radix Glycyrrhizae in the raw meat agent is 1 ︰, 1 ︰ 1,
Described special removing in the raw meat agent also contained tea leaf powder.The addition of tea leaf powder can be 10%～50% of the root of Dahurain angelica, orange peel and Radix Glycyrrhizae gross mass.
Described mixed enzyme is the mixing enzyme preparation that alkali protease, neutral proteinase, papain and trypsase are made with the weight ratio of 1 ︰, 1 ︰, 2 ︰ 1.The consumption of described mixing enzyme preparation is 10g/kg.The consumption of described lipase is 5g/kg.
Following form is embodiment of the invention four-product and the contrast of fish-bone calcium powder product abroad
Claims (7)
1. utilize bony fish fish spine to produce the method for activated calcium powder, may further comprise the steps:
1. put into reaction vessel after bony fish fish spine being cleaned, lipase is added water to submerge, and transferring pH is 7.4～7.8, is warming up to 45～55 ℃, reacts;
2. the fish spine is taken out, crushed after being dried becomes particle diameter at the coarse granule of 2～5mm, bind up with gauze after the raw meat agent evenly mixes with the special weight ratio except raw meat agent=1 ︰ 0.5～2 of fish spine Cu Ke Li ︰ with special removing then, place water to boil 1～2 hour, discard water, get coarse granule and the special mixture except the raw meat agent of fish spine, sieve, remove special for the raw meat agent;
3. with dry after the coarse granule rinsing of fish spine, be ground into the following particulate of particle diameter 1mm, use the mixed enzyme hydrolysis then, filter reserved filtrate after the hydrolysis;
4. described filtrate is made powder by spray-dired mode, namely get activated calcium powder;
Described special except the raw meat agent be to be the powdered substance that main component is made with the Chinese medicine root of Dahurain angelica, orange peel, Radix Glycyrrhizae.
2. a kind of method of utilizing bony fish fish spine to produce activated calcium powder according to claim 1 is characterized in that: described special for also having tea leaf powder in the raw meat agent.
3. a kind of method of utilizing bony fish fish spine to produce activated calcium powder according to claim 1 and 2 is characterized in that: described mixed enzyme is the mixing enzyme preparation that alkali protease, neutral proteinase, papain, trypsase are made with the weight ratio of 1 ︰, 1 ︰, 1 ︰ 1.
4. a kind of method of utilizing bony fish fish spine to produce activated calcium powder according to claim 3, it is characterized in that: the consumption of described mixing enzyme preparation is 5～10 g/kg.
5. a kind of method of utilizing bony fish fish spine to produce activated calcium powder according to claim 1, it is characterized in that: the consumption of described lipase is 1～5 g/kg.
6. a kind of method of utilizing bony fish fish spine to produce activated calcium powder according to claim 1 is characterized in that: described special weight ratio except the root of Dahurain angelica, orange peel, Radix Glycyrrhizae in the raw meat agent is 2 ︰, 2 ︰ 1.
7. a kind of method of utilizing bony fish fish spine to produce activated calcium powder according to claim 1 is characterized in that: described special weight ratio except the root of Dahurain angelica, orange peel, Radix Glycyrrhizae in the raw meat agent is 2 ︰, 1 ︰ 1.
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| CN102871125A (en) * | 2012-10-10 | 2013-01-16 | 天狮集团有限公司 | Bone calcium extraction method capable of reducing fat content, and osteocalcin |
| CN102860514B (en) * | 2012-10-10 | 2014-03-05 | 天狮集团有限公司 | Bone calcium extraction method and bone gla protein |
| CN102871122B (en) * | 2012-10-10 | 2014-05-14 | 天狮集团有限公司 | Method for extracting bone calcium through defatting fermentation and bone gla protein |
| CN102871123B (en) * | 2012-10-10 | 2014-05-14 | 天狮集团有限公司 | Method for preparing bone calcium and bone gla protein |
| CN102871124B (en) * | 2012-10-10 | 2014-05-14 | 天狮集团有限公司 | Low-fat bone calcium extracting method and bone gla protein |
| CN102871128B (en) * | 2012-10-10 | 2014-05-14 | 天狮集团有限公司 | Process for preparing bone calcium and bone gla protein |
| CN103284157B (en) * | 2013-06-07 | 2015-01-28 | 浙江老州山海洋生物科技有限公司 | A preparation method for fish bone calcium soluble granules |
| CN103798845A (en) * | 2014-03-05 | 2014-05-21 | 青岛金海源食品有限公司 | Method for developing nano-calcium collagen by utilizing cod bones |
| CN104522290A (en) * | 2014-12-10 | 2015-04-22 | 内蒙古天奇生物科技有限公司 | Method for preparing bone polypeptide through non-solvent degreasing |
| JP6704053B2 (en) * | 2016-01-26 | 2020-06-03 | タイ ユニオン グループ パブリック カンパニー リミテッド | How to prepare fish bone powder |
| CN105795315A (en) * | 2016-04-08 | 2016-07-27 | 中国水产科学研究院南海水产研究所 | Deodorizing and degreasing method of bass slices |
| CN105876660A (en) * | 2016-04-25 | 2016-08-24 | 西南科技大学 | Method for preparing yak-sheep bone powder by utilizing enzyme method |
| CN106174379A (en) * | 2016-07-20 | 2016-12-07 | 大连工业大学 | A kind of preparation method of high calcium fishbone flavouring base material powder |
| CN106858611B (en) * | 2017-03-03 | 2020-05-22 | 江西师范大学 | Method for preparing peptide calcium by using fishbone |
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Denomination of invention: Preparation of active calcium powder from the backbone of teleost fishes Effective date of registration: 20220728 Granted publication date: 20130710 Pledgee: Nanxun Zhejiang rural commercial bank Limited by Share Ltd. and Fu sub branch Pledgor: HUZHOU ZHENLU BIOLOGICAL PRODUCTS Co.,Ltd. Registration number: Y2022330001550 |