CN103798845A - Method for developing nano-calcium collagen by utilizing cod bones - Google Patents
Method for developing nano-calcium collagen by utilizing cod bones Download PDFInfo
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- 239000011575 calcium Substances 0.000 title claims abstract description 59
- 229910052791 calcium Inorganic materials 0.000 title claims abstract description 59
- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 31
- 102000008186 Collagen Human genes 0.000 title claims abstract description 20
- 108010035532 Collagen Proteins 0.000 title claims abstract description 20
- 229920001436 collagen Polymers 0.000 title claims abstract description 20
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 56
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 102000004190 Enzymes Human genes 0.000 claims abstract description 9
- 108090000790 Enzymes Proteins 0.000 claims abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 9
- 238000002360 preparation method Methods 0.000 claims abstract description 8
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims abstract description 7
- 230000002779 inactivation Effects 0.000 claims abstract description 7
- 229940083466 soybean lecithin Drugs 0.000 claims abstract description 7
- 239000002502 liposome Substances 0.000 claims abstract description 5
- 239000002105 nanoparticle Substances 0.000 claims abstract description 5
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- 229910052698 phosphorus Inorganic materials 0.000 description 5
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- 208000001132 Osteoporosis Diseases 0.000 description 4
- 108010048734 sclerotin Proteins 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
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- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
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- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
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- 235000007164 Oryza sativa Nutrition 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- QXDMQSPYEZFLGF-UHFFFAOYSA-L calcium oxalate Chemical compound [Ca+2].[O-]C(=O)C([O-])=O QXDMQSPYEZFLGF-UHFFFAOYSA-L 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
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- 210000004051 gastric juice Anatomy 0.000 description 1
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- 210000000936 intestine Anatomy 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
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- 235000009566 rice Nutrition 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
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- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/20—Fish extracts
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/65—Addition of, or treatment with, microorganisms or enzymes
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Abstract
The invention relates to a method for developing nano-calcium collagen by utilizing cod bones. The method comprises the following steps: cleaning cod axial bones, coarse crushing, deodorizing and the like; performing fine processing; performing fine crushing on the cod bones by using a mechanical crushing machine; after hydrolysis and enzyme inactivation are performed twice, adding soybean lecithin into an enzymatic solution; the enzymatic solution is in an emulsified state after being impacted strongly by using a high-pressure homogenizer, so that nutrients are coated with a layer of phospholipid membrane, and calcium collagen becomes a liposome; crushing emulsified liquid by using ultra-high-pressure circulating air flow, so that the emulsified liquid is relatively refined and homogeneous until the emulsified liquid is in nano-scale; instantaneously drying by spray-drying to obtain bone collagen calcium nano-particle powder. By adopting the method disclosed by the invention, the nutrients in the nano-calcium collagen are prevented from loss and degeneration; a preparation is prepared to be in the nano-scale, so that the nutrients are relatively easy to absorb, and the preparation can be added into infant milk powder and various types of calcium-supplement foods and nourishments.
Description
Technical field:
The present invention relates to a kind of method of utilizing cod bone exploitation nanometre collagen albumen calcium, belong to biological processing technical field.
Background information:
It is reported, the calcium intake that in all kinds of crowds of China, the intake of calcium is recommended than Chinese Soclety of Nutrition is generally low, and within 60 years old, above old man, particularly elderly woman approximately 80% has osteoporosis symptom, and osteoporosis has become a kind of common disease and frequently-occurring disease.The conventional osteoporosis method of preventing and treating is to replenish the calcium, and has in the market many calcium supplementing products, and these calcium products progressively form two kinds of sorting techniques, and a kind of is to be divided into four classes according to the character of calcium salt: organic calcium, inorganic calcium, Chinese medicine calcium preparation and calcium regulator; Another kind of sorting technique is to be divided three classes according to the development of calcium agent: first generation calcium of organic acid, calcium gluconae etc.; Second on behalf of calcium activated and the preparation take calcium activated as primary raw material; The third generation is Ultramicro-powder calcium carbonate.These calcium agent are difficult to be absorbed by the body a bit, and some oxalic acid easily and in food, the tartaric acid in fruit form calcium oxalate etc., and some has side effect.
Research shows, forming osteoporosis is not singly the loss of calcium, also has the loss of ossein and the loss of phosphorus simultaneously.In the time running off 20% calcium, will there is 80% ossein to run off and a certain proportion of phosphorus loss.Therefore, only pay attention to the supplementary of no avail of simple calcium, produce effects very few, only have the ossein of supplementary rational proportion and calcium, phosphorus, avoid the interference of hydrochloric acid in gastric juice in phosphoric acid in fruits and vegetables, oxalic acid, phytic acid, tartaric acid and stomach simultaneously, be only the mode of the most reasonably replenishing the calcium.
Mend and so on Chinese medicine theory according to what lacks, loss be sclerotin, the sclerotin composition of nourishing is reasonably, wherein fish-bone is the good natural products of replenishing the calcium, and contains abundant nutritional labeling in fish-bone, is mainly made up of ash content, protein, fat.Cod is cold water abyssal fishes, the harm that can avoid fresh-water fishes to bring because of river, pond water quality pollution and terrestrial animal.According to one's analysis, in cod axial bone ash content, calcium content is 200g/kg, phosphorus 113g/kg, iron 49mg/kg, zinc 98mg/kg, iodine 37mg/kg.In protein, 80% is bone collagen, and calcium content in cod bone is that in all fish, content is higher.The overwhelming majority in cod bone is the required nutriment of calcium replenishing and mineral element, but because cod sclerotin is hard, be difficult to be eaten by people, usually used as leftover bits and pieces by low value processing.
In fact, many countries abroad, leftover bits and pieces after aquatic products processing is according to its biochemical characteristic and nutritional labeling classification processing and utilization, the output value of its deep processed product may be several times to tens times of flesh of fish value, the a large amount of cod fillet of the annual processing of China, after processing, a large amount of fish-bones is not fully utilized.
Summary of the invention:
The object of the invention is to overcome the shortcoming of prior art, a kind of method of utilizing cod bone exploitation nanometre collagen albumen calcium is provided.
In order to realize foregoing invention object, the inventive method is take cod bone as raw material, and through lipid, nanometer, without chemical treatment, makes fish-bone keep original nutritive and health protection components and make ossein calcium nano.Technological process of the present invention is:
One, the pretreatment of cod bone:
1, the axle bone in cod: the fish-bone after processing removes the fish gruel being attached on bone;
2, clean: with pure water cleaning, thoroughly remove the impurity being attached on fish-bone;
3, coarse crushing: with the broken bone of broken bone machine, the granularity that makes bone is 0.5cm;
4, de-raw meat: with high pressure steam process, be that 230 ℃-270 ℃, pressure are under 0.4KPa and high vacuum condition in temperature, remove raw meat stink substance by steam distillation;
Two, fine-processing technique flow process:
1, fine powder is broken: cod bone is ground into powdery with mechanical crusher;
2, enzymolysis for the first time: in bone meal, add pure water according to solid-liquid ratio 1:1, using NaOH as catalyst, 50 ℃ of left and right, lipase enzymolysis, making fat splitting in fish-bone is triglyceride and free fatty;
3, the enzyme that goes out: improve temperature to 100 ℃ left and right, make lipase inactivation;
4, enzymolysis for the second time: will be hydrolyzed the bone meal of fish oil, regulating temperature is 50 ℃, and pH is 7, adds the mixed protein enzyme preparation that alkali protease and flavor protease mass ratio are 2:1, enzymolysis, bone collagen is decomposed into the protein peptides of amino acid and molecular weight;
5, the enzyme that goes out: heat to more than 80 ℃, make protease inactivation;
6, emulsification: after twice enzymolysis, there is larger variation in the biochemical state in bone, calcium in bone and aliphatic acid, amino acid are combined into fatty acid calcium and amino acid calcium, be organic calcium, add soybean lecithin in enzymolysis liquid, the mass ratio of enzymolysis liquid and soybean lecithin is 2:1, through high pressure homogenizer intense impact, be emulsification shape, make nutritional labeling be coated with last layer immobilized artificial membrane, make gelatine protocalcium become liposome;
7, super-pressure circulating current pulverize: emulsion adds crushing chamber by Venturi nozzle, and high pressure draught sprays into the not runway circulating tube type crushing chamber of isometrical variable curvature through one group of nozzle, accelerate particle make it mutual impact, collision, friction and pulverize; At the effect blanking diverting flow of graded region centrifugal force field, fine grained is discharged by classifier at internal layer; Coarse granule returns and continues circulation pulverizing along down pipe; Make more refinement and homogeneous of emulsion, until reach nanoscale preset requirement;
8, dry: dry instantaneous dry with spraying, make bone collagen calcium nanoparticle powder.
The whole processing technology of the inventive method is all with machinery or enzyme processing, there is no extreme condition, as strong acid, highly basic, chemical reagent, high temperature etc., environmental protection, kept the nutritional labeling in nanometre collagen albumen calcium not run off and sex change, preparation nanometer makes all nutritional labelings be easier to absorb.
The innovative point of the inventive method is to make the hard natural cod bone that is originally difficult to eat, be difficult to digestion through a series of processing technology, is keeping, under the condition of original nutrient content unchanged, making it liposome, nanometer.Nanometre collagen albumen calcium after processing is except containing a large amount of amino acid, calcium, magnesium, phosphorus, still there is the trace element of many needed by human as iron, zinc, iodine, absorption and utilization to calcium in enteron aisle have synergy, and there is good solubility, solution absorption and do not produce precipitation absolutely almost in intestines and stomach.Ultra micro nano particle makes it quickly, through biological barrier---the digestive ferment effect in stomach, through gastrointestinal tract wall, slowly to dissociate calcium in blood, thereby has avoided the calcium concentration too high kidney discharge capacity of bringing in blood to increase or the generation of hypercalcinemia.Guarantee the abundant absorption of calcium constituent, have fabulous bioavailability.In process, except adding phosphatide, all keep original sclerotin composition, so nontoxic, nanometre collagen albumen calcium can add in all kinds of supplementary calcium foods and nutriment relievedly, as add baby milk powder to and various old supplementary calcium food is medium, be not vulnerable to the interference of other factors in food.
Accompanying drawing explanation:
Fig. 1 is cod bone pretreating process flow chart;
Fig. 2 is the process chart of pretreated cod bone fine finishining nanometre collagen albumen calcium.
The specific embodiment:
Below in conjunction with specific embodiments and the drawings, the inventive method is further elaborated.
Embodiment 1,
One, the pretreatment of cod bone: technological process as shown in Figure 1.
1, the axial bone of cod: the fish-bone after processing removes the fish gruel being attached on bone;
2, clean: with pure water employing giant cleaning twice, thoroughly remove the impurity being attached on fish-bone;
3, coarse crushing: with the broken bone of broken bone machine, the granularity that makes bone is 0.5cm, about grain of rice size;
4, de-raw meat: with high pressure steam process, be that 230 ℃-270 ℃, pressure are under 0.4KPa and high vacuum condition in temperature, remove raw meat stink substance by steam distillation;
Two, fine-processing technique flow process: technological process as shown in Figure 2.
1, fine powder is broken: cod bone is ground into powdery with mechanical crusher;
2, enzymolysis for the first time: in bone meal, add pure water according to solid-liquid ratio 1:1, using NaOH as catalyst, 50 ℃ of left and right, lipase enzymolysis 3h, making fat splitting in fish-bone is triglyceride and free fatty;
3, the enzyme that goes out: improve temperature to 100 ℃ left and right, make lipase inactivation;
4, enzymolysis for the second time: will be hydrolyzed the bone meal of fish oil, regulating temperature is 50 ℃, and pH is 7, adds the mixed protein enzyme preparation that alkali protease and flavor protease mass ratio are 2:1, enzymolysis 14 hours, bone collagen is decomposed into the protein peptides of amino acid and molecular weight;
5, the enzyme that goes out: heat to more than 80 ℃, make protease inactivation;
6, emulsification: after twice enzymolysis, there is larger variation in the biochemical state in bone, calcium in bone and aliphatic acid, amino acid are combined into fatty acid calcium and amino acid calcium, be organic calcium, add soybean lecithin in enzymolysis liquid, the mass ratio of enzymolysis liquid and soybean lecithin is 2:1, through high pressure homogenizer intense impact, be emulsification shape, make nutritional labeling be coated with last layer immobilized artificial membrane, make gelatine protocalcium become liposome;
7, super-pressure circulating current pulverize: emulsion adds crushing chamber by Venturi nozzle, and high pressure draught sprays into the not runway circulating tube type crushing chamber of isometrical variable curvature through one group of nozzle, accelerate particle make it mutual impact, collision, friction and pulverize; At the effect blanking diverting flow of graded region centrifugal force field, fine grained is discharged by classifier at internal layer; Coarse granule returns and continues circulation pulverizing along down pipe; Make more refinement and homogeneous of emulsion, until reach nanoscale preset requirement;
8, dry: dry instantaneous dry with spraying, make bone collagen calcium nanoparticle powder.
Claims (1)
1. a method of utilizing cod bone exploitation nanometre collagen albumen calcium, is characterized in that technological process is: the pretreatment of (1) cod bone: the fish-bone after processing removes the fish gruel being attached on bone; With pure water cleaning, thoroughly remove the impurity being attached on fish-bone; With the broken bone of broken bone machine, the granularity that makes bone is 0.5cm; With high pressure steam process, be that 230 ℃-270 ℃, pressure are under 0.4KPa and high vacuum condition in temperature, remove raw meat stink substance by steam distillation; (2) fine-processing technique flow process: cod bone is ground into powdery with mechanical crusher; In bone meal, add pure water according to solid-liquid ratio 1:1, using NaOH as catalyst, at 50 ℃, lipase enzymolysis, making fat splitting in fish-bone is triglyceride and free fatty; Improve temperature to 100 ℃ left and right, make lipase inactivation; To be hydrolyzed the bone meal of fish oil, regulating temperature is 50 ℃, and pH is 7, adds the mixed protein enzyme preparation that alkali protease and flavor protease mass ratio are 2:1, enzymolysis, and bone collagen is decomposed into amino acid and protein peptides; Heat to more than 80 ℃, make protease inactivation; After twice enzymolysis, calcium in bone and aliphatic acid, amino acid are combined into fatty acid calcium and amino acid calcium, in enzymolysis liquid, add soybean lecithin, the mass ratio of enzymolysis liquid and soybean lecithin is 2:1, through high pressure homogenizer intense impact, be emulsification shape, make nutritional labeling be coated with last layer immobilized artificial membrane, make gelatine protocalcium become liposome; Emulsion adds crushing chamber by Venturi nozzle, and high pressure draught sprays into the not runway circulating tube type crushing chamber of isometrical variable curvature through one group of nozzle, accelerate particle make it mutual impact, collision, friction and pulverize; At the effect blanking diverting flow of graded region centrifugal force field, fine grained is discharged by classifier at internal layer; Coarse granule returns and continues circulation pulverizing along down pipe; Make more refinement and homogeneous of emulsion, until reach nanoscale; Dry instantaneous dry with spraying, make bone collagen calcium nanoparticle powder.
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| CN105713785A (en) * | 2016-03-25 | 2016-06-29 | 浙江海洋学院 | Squid bone rice wine and preparation method thereof |
| CN105832697A (en) * | 2016-04-19 | 2016-08-10 | 海南海神同联生物科技开发有限公司 | Preparation method and application of vitamin D3 calcium soft capsule |
| CN106047970A (en) * | 2016-06-21 | 2016-10-26 | 山东东方海洋科技股份有限公司 | Extraction method of collagen polypeptide and active calcium in walleye pollock bone |
| CN106047970B (en) * | 2016-06-21 | 2019-06-04 | 山东东方海洋科技股份有限公司 | The extracting method of collagen polypeptide and calcium activated in wall pollack fish-bone |
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| CN107006862A (en) * | 2017-04-01 | 2017-08-04 | 吉林农业大学 | A kind of health products for strengthening bone density by raw material of Rana sylvatica Le conte bone and preparation method thereof |
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