CN100506063C - Method for treating defatted bean-dregs and product therefrom - Google Patents
Method for treating defatted bean-dregs and product therefrom Download PDFInfo
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- CN100506063C CN100506063C CNB2006100866279A CN200610086627A CN100506063C CN 100506063 C CN100506063 C CN 100506063C CN B2006100866279 A CNB2006100866279 A CN B2006100866279A CN 200610086627 A CN200610086627 A CN 200610086627A CN 100506063 C CN100506063 C CN 100506063C
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Abstract
A method for processing the defatted soybean dregs as the by-product generated by extracting soybean isoflavone from defatted soybean cake features that said fatted soybean dregs are hydrolyzed by use of neutral proteinase, pectinase and cellulase to obtain soybean fiber product with high content of soluble fiber.
Description
Technical field
The present invention relates to food processing field, relate in particular to the processing and treating method and the products obtained therefrom thereof of bean product.
Background technology
Usually use de-fatted soybean dregs to extract isoflavones, but often that it is discarded to the processing of the accessory substance defatted bean-dregs of this process, cause waste significantly like this, and improved production cost.
Up to the present, the extraction preparation method of soybean dietary fiber is mainly as follows: with raw material, for example fresh bean dregs, skin of beancurd is main, use earlier papain, lipase hydrolysis is handled protein and the fat of removing wherein, be to stir modulation and the solvable composition of elimination under 9 the condition at PH then, with insoluble matter with 0.04% cellulose hydrolyzation 1～24 hour, obtain the fibre and soya product, carry out post processing again, for example dry, pulverize etc., wherein drying can adopt flash distillation, oven dry, microwave drying is controlled at 4～10% with moisture, and pulverizing can be adopted common pulverizing or ultramicro grinding.In the finished product of Ti Quing, fiber content is in 50～70% scopes like this, and Soluble Fiber content can be used as the intermediate products use that processed food adds in 10～14% scopes.
For example application number is that 93119191.2 Chinese patent application discloses a kind of fibrous matter that improves the solubility dietary fiber content and preparation method thereof, thus the PH that specifically comprises the fibre-bearing slurry that soybean is made transfer to 4～5 then heating improve Soluble Fiber content.Being still fresh bean product but this method is raw materials used, is not defatted bean-dregs.
Summary of the invention
The object of the present invention is to provide a kind of processing method of defatted bean-dregs, can obtain the higher fibre and soya product of Soluble Fiber content by this method.
The objective of the invention is to be achieved through the following technical solutions.
Main design of the present invention is, since not fatty and protein,alcohol-soluble content is low in the defatted bean-dregs, so it is handled, thereby can obtain the high fibre and soya product of Soluble Fiber content by neutral proteinase, pectase, cellulose hydrolyzation.
Particularly, defatted bean-dregs processing method provided by the invention comprises: use neutral proteinase to be hydrolyzed defatted bean-dregs, products therefrom is gone out to be hydrolyzed simultaneously with pectase and cellulase behind the enzyme, then can obtain the high product of Soluble Fiber content.
Wherein, defatted bean-dregs is the accessory substance of gained behind the de-fatted soybean dregs extraction isoflavones, wherein Zhi Fang residual quantity generally is lower than 0.5wt%, therefore do not need wherein fat to be removed in its hydrolysis with lipase, therefore and pure protein content wherein is also lower, thereby can directly utilize neutral proteinase to make its hydrolysis remove wherein protein ingredient.For efficient and the degree that improves hydrolysis, preferably defatted bean-dregs is carried out preliminary treatment, for example, its extruding removed residual ethanol after, add the water mill slurry, colloid mill is pulverized and the sterilization deodorization.
The hydrolysising condition of neutral proteinase generally can for: PH7.0, temperature are that 40～60 ℃, institute's enzyme concentration are 0.1～0.5wt% (based on the weight of hydrolysis substance dry).Because the hydrolysis efficiency of neutral proteinase is higher than papain and trypsase, so hydrolysis effect is relatively good, the albumen residual quantity in the hydrolysis products therefrom generally can be reduced to 1～5wt% (dry matter weight meter).The general hydrolysis number of times that does not limit neutral proteinase, for the comprehensive angle of cost and hydrolysis effect, twice of selective hydrolysis.
The enzyme that goes out of neutral proteinase hydrolysis products therefrom can pass through, for example sterilization, and for example the mode of heating, irradiation is carried out.
Being the galactolipin structure more than 60% in the fibre and soya, is main component with pectin class polysaccharide, therefore adopts the recovery rate and the eating mouth feel of pectase and cellulase hydrolysis simultaneously can raising fibre and soya.
The used pectase of the present invention can be this area pectase commonly used, and is not particularly limited, for example pectin lyase, polygalacturonase, pectate lyases and pectinesterase etc., and these enzymes may be used alone, can also be used in combination.
The used cellulase of the present invention can be this area cellulase commonly used, and is not particularly limited, for example endoglucanase, cellobiohydrolase and beta-glucosidase etc., and these enzymes may be used alone, can also be used in combination.
The condition of pectase and cellulase hydrolysis simultaneously generally can be PH5～8, temperature is 30～50 ℃, pectase consumption 0.2～0.3wt% (based on the weight of hydrolysis substance dry), cellulase consumption 0.15～0.2wt% (based on the weight of hydrolysis substance dry).
The product that obtains by said method (is the paste attitude, if want to improve its useful life, can carry out processing such as sterilizing and drying to obtain desciccate) in, the Soluble Fiber cellulose content is higher, generally can reach 15～20%, total dietary fiber can reach (all in dry) more than 90%, so range of application is very extensive, can be used for producing fibre and soya food, for example be used to produce the product of fibre and soya electuary class.
For example above-mentioned processing products obtained therefrom is mixed to obtain the direct-edible electuary product of functional form with nutritional labelings such as lactalbumin and other vitamin or trace elements, wherein the weight proportion of various compositions is preferably:
Above-mentioned processing products obtained therefrom: 80～90 weight portions (in butt)
Lactalbumin: 10～20 weight portions
Vitamin A: 700～800U/100g (to the material total amount)
Vitamin E: 0.6～1.2mg/100g (to the material total amount)
Calcium lactate: 1000～1500mg/100g (in calcium ion to the material total amount)
Ferrous lactate: 8～12mg/100g (in iron ion to the material total amount)
Zinc gluconate: 4～6mg/100g (in zinc ion to the material total amount)
Product is dispersed very good behind the gained electuary product drying, dispersion index is up to more than 80%, wherein natural plant fibre content height also contains and strengthens the physical lactalbumin and the trace element and the vitamin of some needed by human, is the well edible object of body beautification women.
The accessory substance defatted bean-dregs that the present invention uses defatted soybean meal to extract isoflavones is raw material, improve twice laid efficient, reduced cost, improved the recovery rate and the content of soybean dietary fiber with the combination enzymatic hydrolysis, fiber and Soluble Fiber content height in the products obtained therefrom, good dispersion.
The specific embodiment
Below further describe the present invention by specific embodiment; but these embodiment only are illustrative; protection scope of the present invention is not constituted any influence; it will be understood by those skilled in the art that any modification of making according to spirit of the present invention and replace and all fall within the scope of protection of the present invention.
Embodiment 1
1. get the filter residue that pure method defatted soybean meal extracts isoflavones, remove residual ethanol with plate compression;
2. get gained bean dregs among the 1kg1,, it is ground, form beans and stick with paste with kibbling mill to wherein adding 4kg water;
3. beans are stuck with paste and further ground (the colloid mill aperture is 0.2mm) with colloid mill;
4. beans are stuck with paste and put into sterilization deodorization unit, under 125 ℃ temperature, handled 10 seconds;
5. sticking with paste with neutral proteinase hydrolysis beans, is that 40 ℃, neutral proteinase are hydrolysis 12 hours under the condition of 5.0g in PH7.0, temperature, and the same terms repeats hydrolysis 1 time again;
6. the beans after the hydrolysis are stuck with paste the enzyme 20 minutes of under 90 ℃ temperature, going out, removed by filter DDGS then;
7. stick with paste with gained beans in pectin lyase (pectase), endoglucanase (cellulase) hydrolysis 5 simultaneously, hydrolysising condition is: PH5, and temperature is 50 ℃, hydrolysis time 12 hours, pectase consumption 2.0g, cellulase consumption 1.5g;
8. sterilization: sterilization is 20 minutes under 95 ℃ temperature, obtains product.
Embodiment 2
Embodiment 1 products obtained therefrom is got 80g (in dry), get lactalbumin 20g, 800mg vitamin A, 1mg vitamin E, 1200mg calcium lactate (in calcium ion), 10mg ferrous lactate (in iron ion), 5mg zinc gluconate (in zinc ion) again, homogeneous is 10 minutes under the pressure of 20MP, concentrate, spray-drying (160 ℃ of inlet temperatures, 80 ℃ of outlet temperatures), obtain the fibre and soya electuary.
Embodiment 3
Handle the defatted bean-dregs preparing product with the method identical with embodiment 1, its difference only is: the condition of use neutral proteinase hydrolysis is: PH7.0, and temperature is 60 ℃, neutral proteinase is 1.0g; Condition when using pectase and cellulose hydrolyzation is: PH is 8, and temperature is 30 ℃, and pectase is 2.5g, and cellulase is 1.8g, and used pectase is specially pectinesterase, and cellulase is a cellobiohydrolase.
Embodiment 4
Embodiment 3 products obtained therefrom 90g are mixed with lactalbumin 10g, 700mg vitamin A, 1.2mg vitamin E, 1000mg calcium lactate (in calcium ion), 12mg ferrous lactate (in iron ion), 4mg zinc gluconate (in zinc ion), homogeneous is 3 minutes under the pressure of 30MP, concentrate, spray-drying (170 ℃ of inlet temperatures, 90 ℃ of outlet temperatures), obtain the fibre and soya electuary.
Embodiment 5
Handle the defatted bean-dregs preparing product with the method identical with embodiment 1, its difference only is: the condition of use neutral proteinase hydrolysis is: PH7.0, and temperature is 50 ℃, neutral proteinase is 2.0g; Condition when using pectase and cellulose hydrolyzation is: PH is 6, and temperature is 45 ℃, and pectase is 3.0g, and cellulase is 2.0g, and used pectase is specially polygalacturonase, and cellulase is a beta-glucosidase.
Embodiment 6
Embodiment 5 products obtained therefrom 85g are prepared the fibre and soya electuary with the method identical with embodiment 2, and its difference only is: used whey albumen is 15g.
Claims (9)
1. the processing method of a defatted bean-dregs, this method comprises: use neutral proteinase to be hydrolyzed defatted bean-dregs, products therefrom is gone out to be hydrolyzed simultaneously with pectase and cellulase behind the enzyme.
2. require 1 method according to profit, it is characterized in that described defatted bean-dregs is meant and undertaken by the bean dregs that obtain after extracting isoflavones from de-fatted soybean dregs that preliminary treatment obtains, described preliminary treatment is for after extruding removes residual ethanol, add the water mill slurry, colloid mill is pulverized and the sterilization deodorization.
3. require 1 or 2 method according to profit, it is characterized in that the hydrolysising condition of neutral proteinase is: PH7.0, temperature are that 40～60 ℃, the amount of neutral proteinase are 0.1～0.5wt%, based on the weight of hydrolysis substance dry.
4. require 1 or 2 method according to profit, it is characterized in that, described pectase is one of pectin lyase, polygalacturonase, pectate lyases and pectinesterase or its mixture.
5. require 1 or 2 method according to profit, it is characterized in that, described cellulolytic enzyme is one of endoglucanase, cellobiohydrolase and beta-glucosidase or its mixture.
6. require 1 or 2 method according to profit, it is characterized in that, the condition of pectase and cellulase hydrolysis simultaneously is: PH5～8, temperature is 30～50 ℃, pectase consumption 0.2～0.3wt%, based on the weight of hydrolysis substance dry, cellulase consumption 0.15～0.2wt% is based on the weight of hydrolysis substance dry.
7. the product that obtains of each described method of claim 1-6.
8. the described product of claim 7 is in preparation fibre and soya Application in Food.
9. a fibre and soya electuary is characterized in that, wherein the weight proportion of each composition is:
The described product of claim 7: 80～90 weight portions, in butt
Lactalbumin: 10～20 weight portions
Vitamin A: 700～800U/100g is to the material total amount
Vitamin E: 0.6～1.2mg/100g is to the material total amount
Calcium lactate: 1000～1500mg/100g, in calcium ion to the material total amount
Ferrous lactate: 8～12mg/100g, in iron ion to the material total amount
Zinc gluconate: 4～6mg/100g, in zinc ion to the material total amount.
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| CNB2006100866279A CN100506063C (en) | 2006-06-23 | 2006-06-23 | Method for treating defatted bean-dregs and product therefrom |
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| CNB2006100866279A CN100506063C (en) | 2006-06-23 | 2006-06-23 | Method for treating defatted bean-dregs and product therefrom |
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| CN100506063C true CN100506063C (en) | 2009-07-01 |
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Cited By (2)
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| CN103222650A (en) * | 2013-04-23 | 2013-07-31 | 广西轻工业科学技术研究院 | Bean dreg minced fillet instant foodstuff and preparation method thereof |
| CN107960479A (en) * | 2017-11-23 | 2018-04-27 | 山东渤海实业股份有限公司 | A kind of method for preparing soybean protein food and manufactured soybean protein food |
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| CN101530162B (en) * | 2008-03-10 | 2011-11-23 | 北京大北农科技集团股份有限公司 | Preparation method of novel protein feed additive |
| CN101961093B (en) * | 2010-10-22 | 2012-07-25 | 哈尔滨工业大学 | Preparation method of active soybean dietary supplement |
| CN103960331A (en) * | 2013-01-29 | 2014-08-06 | 陈成 | Nutrition breakfast bean dregs cake prepared in enzymic method |
| CN103766575B (en) * | 2014-02-28 | 2015-05-13 | 北京金地三福膨化机制造(大厂)有限公司 | Method for preparing modified soybean protein from soybean pulp |
| CN104256660A (en) * | 2014-09-16 | 2015-01-07 | 山东省高唐蓝山集团总公司 | Method for reducing protein content in soybean dietary fiber |
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| CN108316036A (en) * | 2018-01-25 | 2018-07-24 | 陕西科技大学 | A kind of method that bean dregs prepare fento cellulose |
| CN108771134A (en) * | 2018-06-25 | 2018-11-09 | 山东禹王生态食业有限公司 | A kind of method of soluble dietary fiber in enzyme assisted extraction bean dregs |
| CN108929892B (en) * | 2018-08-02 | 2021-12-10 | 扬州市龙伟食品有限公司 | Method for extracting soluble bean dregs protein and polypeptide from bean dregs |
| CN109251233B (en) * | 2018-09-21 | 2021-11-12 | 贵州省三好食品开发有限公司 | Method for extracting bean protein from bean dregs |
| CN114885969A (en) * | 2022-04-28 | 2022-08-12 | 安徽八公山豆制品有限公司 | Bean dreg biscuit rich in isoflavone and preparation method thereof |
| CN115868603A (en) * | 2022-12-29 | 2023-03-31 | 东莞市华井生物科技有限公司 | Enzymolysis bean dreg powder and preparation method thereof |
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| CN107960479A (en) * | 2017-11-23 | 2018-04-27 | 山东渤海实业股份有限公司 | A kind of method for preparing soybean protein food and manufactured soybean protein food |
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| CN1860906A (en) | 2006-11-15 |
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