CN109251233B - Method for extracting bean protein from bean dregs - Google Patents
Method for extracting bean protein from bean dregs Download PDFInfo
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- CN109251233B CN109251233B CN201811110329.8A CN201811110329A CN109251233B CN 109251233 B CN109251233 B CN 109251233B CN 201811110329 A CN201811110329 A CN 201811110329A CN 109251233 B CN109251233 B CN 109251233B
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
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Abstract
The invention belongs to the field of bean dregs processing, and particularly discloses a method for extracting bean protein from bean dregs, which comprises the steps of bean dregs pretreatment, enzyme treatment, ultrasonic extraction, centrifugal separation, acidification treatment and the like.
Description
Technical Field
The invention belongs to the field of bean dregs processing, and particularly relates to a method for extracting bean protein from bean dregs.
Background
Soybeans are edible and oil-extracting, and are called "nutritious flowers", "king in beans" and "meat in the field", which are the most popular foods among hundreds of natural foods for nutritionists. In current soybean production processes, the fat and protein fractions are mainly utilized. A large amount of bean dregs are generated in the processing process of the soybean oil and the soybean protein, for example, 30 to 35 percent of the bean dregs are generated in the processing of the soybean protein isolate. The main component of the bean dregs is the cell wall of the cotyledon part, the cell wall contains about 30 percent of soybean polysaccharide protein (namely bean protein) based on the bean dregs, and the soybean polysaccharide protein has high nutritive value and good health care function. However, the soybean polysaccharide protein exists in the cell wall of the soybean and is tightly combined with the cell wall, and the soybean polysaccharide protein is difficult to obtain by a common physical processing method.
Disclosure of Invention
The invention aims to provide a method for extracting soy protein from bean dregs so as to solve the problem of extracting the soy protein from the bean dregs.
In order to achieve the purpose, the basic scheme of the invention is as follows: a method for extracting soy protein from bean dregs comprises the following steps:
A. pretreatment of bean dregs:
heating fresh bean dregs, and crushing the heated bean dregs into 20-50 meshes;
B. enzyme treatment:
putting the crushed bean dregs into a collection container, adding 5-20% of cellulase solution and 3-8% of pectinase solution into the collection container, wherein the weight ratio of the cellulase solution to the pectinase solution to the bean dregs is 1:2:10, and uniformly mixing and stirring to obtain a mixture;
C. ultrasonic extraction:
b, adding the mixture obtained in the step B into an ultrasonic extractor for ultrasonic extraction to obtain an extracting solution;
D. centrifugal separation:
c, placing the extracting solution obtained in the step C into a centrifuge for centrifugal treatment to obtain supernatant and precipitate;
E. acidifying:
and D, adding acid liquor into the supernatant obtained in the step D for reaction, and obtaining a precipitate which is the soy protein.
The theory of operation and the beneficial effect of this basic scheme lie in:
and B, adding a cellulase solution and a pectinase solution for enzyme treatment, wherein the main components of the plant cell wall are cellulose and pectin, and the cellulase solution and the pectinase respectively perform enzymolysis reaction with the cellulose and the pectin to further destroy the cell wall. In the case of more bean dregs, the enzymolysis reaction is insufficient, so that the cell wall is further destroyed by using an ultrasonic method in the step C, so that the protein in the bean dregs is released. The enzymolysis reaction is matched with ultrasonic waves, so that the protein is released more easily. In step D, the liberated protein is separated from other materials by centrifugation.
Further, in the step B, surfactant with the concentration of 70-100ppm is synchronously added with the cellulase solution, and the weight ratio of the surfactant to the cellulase solution is 1: 5.
has the advantages that: the surfactant can promote the dissolution of protein, and can significantly improve the extraction rate of protein in the bean dregs by being matched with enzymolysis.
Further, in the step C, the ultrasonic extraction conditions are as follows: the ultrasonic power is 500-800W, the temperature is 40-80 ℃, and the time is 20-30 minutes.
Has the advantages that: under the limited condition, a better extraction effect can be obtained, the ultrasonic power is not too high, otherwise, the structure of the extracted soybean protein is easy to damage.
Further, in the step A, the heating temperature was 50 ℃ and the heating time was 1 hour.
Has the advantages that: the main reason for heating in the step A is to dry fresh wet bean dregs, and under the limited condition, a better drying effect can be obtained.
Further, in step B, the surfactant is a nonionic surfactant.
Has the advantages that: the nonionic surfactant is not ionized in the solution, the structure is more stable, and the phenomenon that the surfactant is ionized into ions to influence the effect of the surfactant due to the fact that moisture still exists in the bean dregs is avoided.
Drawings
FIG. 1 is a sectional view of a crushing apparatus used in an example of the present invention, with a longitudinal section of the crushing apparatus taken as a cross section;
FIG. 2 is a sectional view taken along line A-A of FIG. 1;
fig. 3 is a sectional view taken along line B-B in fig. 1.
Detailed Description
The following is further detailed by way of specific embodiments:
reference numerals in the drawings of the specification include: the piston type piston pump comprises a cover plate 1, a bottom plate 2, a corrugated pipe 3, a rotating shaft 4, a nut 5, a blade 6, a first piston cylinder 7, a support rod 8, a connecting rod 9, a first through hole 10, a second piston cylinder 11, a second piston rod 12 and a second through hole 13.
A method for extracting soy protein from bean dregs comprises the following steps:
A. pretreatment of bean dregs:
heating fresh bean dregs at 50 deg.C for 1 hr, and pulverizing the heated bean dregs to 40 mesh.
B. Enzyme treatment:
putting the crushed bean dregs into a collection container, adding a cellulase solution with the concentration of 10%, a pectinase solution with the concentration of 5% and a surfactant with the concentration of 80ppm into the collection container, wherein the weight ratio of the surfactant to the cellulase solution to the pectinase solution to the bean dregs is 1:5:10:50, the surfactant is a nonionic surfactant, and the surfactant is alkylolamide specifically, and uniformly mixing and stirring to obtain a mixture.
C. Ultrasonic extraction:
and C, placing the mixture obtained in the step B into an ultrasonic extractor for ultrasonic extraction, wherein the ultrasonic extraction conditions are as follows: the ultrasonic power is 700W, the temperature is 60 ℃, and the time is 25 minutes, so that the extracting solution is obtained.
D. Centrifugal separation:
and D, placing the extracting solution obtained in the step C into a centrifuge for centrifugal treatment to obtain supernatant and precipitate.
E. Acidifying:
and D, adding acid liquor into the supernatant obtained in the step D for reaction, wherein the acid liquor is citric acid, and the obtained precipitate is soy protein.
A crushing device is needed when the bean dregs are pretreated in the step A. As shown in figure 1, the crushing device comprises a frame, a bottom plate 2 is fixed on the frame, and the edge of the bottom plate 2 protrudes upwards to form a bottom frame. Be fixed with bellows 3 on the frame of bottom, bellows 3 upper end is fixed with apron 1, 1 marginal downward protrusion in apron forms the top frame, 1 vertical sliding connection of apron is in the frame, be equipped with in the frame and carry out spacing slide rail to apron 1, the slide rail is injectd apron 1 and can only vertical slip, and is injectd the slip orbit of apron 1, avoids apron 1 crooked, and the frame is all carried on spacingly to apron 1 and bottom plate 2, therefore crooked phenomenon can not take place for bellows 3. Be equipped with the feed inlet on the apron 1, sliding connection has the baffle that is used for blocking the feed inlet on the apron 1, and the baffle size is greater than the size of feed inlet.
The rotating connection has pivot 4 on the bottom plate 2, and 4 upper ends of pivot run through apron 1 and extend to outside the apron 1, are fixed with the motor in the frame, and the output shaft of motor is in the same place with 4 upper ends of pivot are fixed, and the motor is used for driving 4 rotations of pivot. The upper portion of the rotating shaft 4 is provided with a forward thread, the forward thread is matched with the thread of the cover plate 1, the lower portion of the rotating shaft 4 is provided with a reverse thread, the reverse thread is connected with a nut 5 in a threaded manner, and the nut 5 is provided with blades 6 along the circumferential direction.
Be fixed with second piston cylinder 11 on bottom plate 2, 11 lower extremes of second piston cylinder are sealed, and sliding connection has the second piston in the second piston cylinder 11, and the second piston upside is fixed with second piston rod 12, and second piston rod 12 upper end runs through bottom plate 2 and extends to in the bottom plate 2, and second piston rod 12 upper end fixed connection is at the lower terminal surface of nut 5.
The upper portion of the second piston cylinder 11 is provided with a first air inlet hole and a first air outlet hole, the first air inlet hole is communicated with a first air inlet check valve, and when the air pressure at the upper portion of the second piston cylinder 11 is reduced, external air is supplemented into the second piston cylinder 11 through the first air inlet check valve. The first air outlet hole is internally communicated with a first air outlet one-way valve, when the air pressure at the upper part of the second piston cylinder 11 is increased, the air in the second piston cylinder 11 is discharged through the first air outlet one-way valve, the first air outlet hole is communicated with a first air outlet pipe, a plurality of first through holes 10 are circumferentially arranged on the top frame, and the first air outlet pipe is communicated with the first through holes 10. Referring to fig. 2, the inner ends of the first through holes 10 are inclined as indicated by arrows, i.e., the swirling direction of the gas entering the top frame through the first through holes 10 is as indicated by arrows.
The lower part of the second piston cylinder 11 is provided with a second air inlet hole and a second air outlet hole, the second air inlet hole is communicated with a second air inlet check valve, and when the air pressure at the lower part of the second piston cylinder 11 is reduced, external air is supplemented into the second piston cylinder 11 through the second air inlet check valve. The second air outlet hole is internally communicated with a second air outlet one-way valve, when the air pressure at the lower part of the second piston cylinder 11 is increased, the air in the second piston cylinder 11 is discharged through the second air outlet one-way valve, the second air outlet hole is communicated with a second air outlet pipe, a plurality of second through holes 13 are formed in the bottom frame along the circumferential direction, and the second air outlet pipe is communicated with the second through holes 13. Referring to fig. 3, the inner ends of the second through holes 13 are inclined as indicated by arrows, i.e. the swirling direction of the gas entering the bottom frame through the second through holes 13 is as indicated by arrows.
During specific work, the baffle slides for the feed inlet is opened, and in throwing into bellows 3 with fresh bean dregs through the feed inlet, starter motor, motor drive pivot 4 corotation, forward screw and the cooperation of apron 1, apron 1 upward movement, bellows 3 are tensile. The first piston cylinder 7 moves upward with the cover plate 1. The reverse threads are matched with the nuts 5, the nuts 5 drive the blades 6 to move downwards, the supporting rods 8 move downwards along with the nuts 5, the supporting rods 8 drive the first piston rods to move downwards, the first pistons move downwards along with the first piston rods, the first pistons and the first piston cylinders 7 move in opposite directions, the space below the first piston cylinders 7 is reduced, air pressure is increased, and air is discharged into the corrugated pipe 3. Because the corrugated pipe 3 is stretched, the bean dregs move upwards along with the stretching of the corrugated pipe 3, the gas moves downwards, the gas and the bean dregs move oppositely, and the impact of the gas on the bean dregs can be used for primarily crushing the bean dregs.
The nut 5 drives the blade 6 to move downwards and move opposite to the bean dregs moving upwards, and the bean dregs are further crushed. Meanwhile, the nut 5 drives the second piston rod 12 to move downwards, the second piston moves downwards along with the second piston rod 12, the space at the lower part of the second piston cylinder 11 is reduced, the air pressure is increased, the air is discharged to the lower part of the corrugated pipe 3 through the second air outlet pipe, after the air enters, anticlockwise vortex-shaped air flow shown in figure 3 is formed at the lower part of the corrugated pipe 3, the bean dregs move towards the direction close to the inner wall of the corrugated pipe 3 under the action of the centrifugal force of the rotating shaft 4, the bean dregs are easily influenced by the rotating direction of the rotating shaft 4 and are consistent with the rotating direction of the rotating shaft 4, the rotating shaft 4 rotates forwards, the direction of the air flow formed by the air is opposite to the rotating direction of the rotating shaft 4, the bean dregs are impacted, and crushing is completed.
When the nut 5 moves to a position close to the bottom plate 2, the rotation direction of the motor is changed, and the motor drives the rotating shaft 4 to rotate reversely. The cover plate 1 drives the first piston cylinder 7 to move downwards, the nut 5 moves upwards, the blades 6 move upwards along with the nut 5, bean dregs move downwards along with the contraction of the corrugated pipe 3 due to the contraction of the corrugated pipe 3, and the blades 6 and the bean dregs are opposite in moving direction and crush the bean dregs.
Meanwhile, the first piston rod and the second piston rod 12 both move upwards along with the nut 5, the first piston rod drives the first piston to move upwards, the first piston cylinder 7 and the first piston move in opposite directions, and the external air is extracted from the lower portion of the first piston cylinder 7 and enters the first piston cylinder 7. The second piston rod 12 drives the second piston to move upwards, the space above the second piston cylinder 11 is reduced, the air pressure is increased, the air on the upper part of the second piston cylinder 11 is discharged to the upper part of the corrugated pipe 3, after the air enters, clockwise vortex-shaped air flow is formed on the upper part of the corrugated pipe 3 as shown in figure 2, the bean dregs move towards the direction close to the inner wall of the corrugated pipe 3 under the action of centrifugal force of the rotating shaft 4, and are easily influenced by the rotating direction of the rotating shaft 4 and consistent with the rotating direction of the rotating shaft 4, the rotating shaft 4 rotates reversely at the moment, the air flow direction formed by the air is opposite to the rotating direction of the rotating shaft 4, the bean dregs are impacted and further crushed.
In the process, the motor can rotate forwards and reversely repeatedly, and bean dregs can be thoroughly crushed. This device utilizes the direction difference of air current and 4 motion of pivot, strikes in a plurality of directions that the dregs of beans moved and smashes, and the impact of air current is dried the dregs of beans on the one hand, and the flow of on the other hand air current is avoided the dregs of beans adhesion on the device. The stretching and shrinking of the corrugated pipe 3 realize the up-and-down movement of the first piston cylinder 7 on one hand, so that the bean dregs move up and down, and on the other hand, the bean dregs are prevented from being adhered to the inner wall of the corrugated pipe 3.
Compare current a device that is used for carrying out the pretreatment to the dregs of beans, this device is more thorough to the smashing of dregs of beans, and can also dry the dregs of beans when smashing, reduces the processing step. Through avoiding the adhesion of dregs of beans to reduce the waste of dregs of beans on the device, and avoid the dregs of beans of adhesion on the device to be unable to smash.
The foregoing is merely an example of the present invention and common general knowledge of known specific structures and features of the embodiments is not described herein in any greater detail. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent.
Claims (5)
1. A method for extracting soy protein from bean dregs is characterized by comprising the following steps: the method comprises the following steps:
A. pretreatment of bean dregs:
heating fresh bean dregs, and crushing the heated bean dregs into 20-50 meshes;
B. enzyme treatment:
putting the crushed bean dregs into a collection container, adding 5-20% of cellulase solution and 3-8% of pectinase solution into the collection container, wherein the weight ratio of the cellulase solution to the pectinase solution to the bean dregs is 1:2:10, and uniformly mixing and stirring to obtain a mixture;
C. ultrasonic extraction:
b, adding the mixture obtained in the step B into an ultrasonic extractor for ultrasonic extraction to obtain an extracting solution;
D. centrifugal separation:
c, placing the extracting solution obtained in the step C into a centrifuge for centrifugal treatment to obtain supernatant and precipitate;
E. acidifying:
d, adding acid liquor into the supernatant obtained in the step D for reaction, and obtaining a precipitate which is soy protein; the crushing device is required to be used for the bean dreg pretreatment in the step A and comprises a rack, wherein a bottom plate is fixed on the rack, and the edge of the bottom plate protrudes upwards to form a bottom frame; the bottom frame is fixed with a corrugated pipe, the upper end of the corrugated pipe is fixed with a cover plate, the edge of the cover plate protrudes downwards to form a top frame, the cover plate is vertically and slidably connected to the rack, and the rack is provided with a slide rail for limiting the cover plate; the cover plate is provided with a feed inlet, the cover plate is connected with a baffle plate for blocking the feed inlet in a sliding manner, and the size of the baffle plate is larger than that of the feed inlet;
the bottom plate is rotatably connected with a rotating shaft, and the upper end of the rotating shaft penetrates through the cover plate and extends out of the cover plate; the upper part of the rotating shaft is provided with a forward thread which is matched with the thread of the cover plate, the lower part of the rotating shaft is provided with a reverse thread, the reverse thread is in threaded connection with a nut, and the nut is provided with blades along the circumferential direction;
the upper end face of the nut is fixedly connected with a supporting rod, the upper end of the supporting rod penetrates through the cover plate and extends out of the cover plate, a first piston cylinder is fixed on the cover plate, an air hole communicated with the lower end of the first piston cylinder is formed in the cover plate, and an exhaust check valve is communicated in the air hole; a first piston is connected in the first piston cylinder in a sliding manner, a first piston rod is fixed on the upper side of the first piston, and a connecting rod is fixed between the upper end of the first piston rod and the upper end of the supporting rod;
a second piston cylinder is fixed on the bottom plate, the lower end of the second piston cylinder is sealed, a second piston is connected in the second piston cylinder in a sliding mode, a second piston rod is fixed on the upper side of the second piston, the upper end of the second piston rod penetrates through the bottom plate and extends into the bottom plate, and the upper end of the second piston rod is fixedly connected to the lower end face of the nut; the upper part of the second piston cylinder is provided with a first air inlet hole and a first air outlet hole, the first air inlet hole is communicated with a first air inlet one-way valve, the first air outlet hole is communicated with a first air outlet one-way valve, a plurality of first through holes are formed in the top frame along the circumferential direction, and the first air outlet pipe is communicated with the first through holes;
the lower part of the second piston cylinder is provided with a second air inlet hole and a second air outlet hole, a second air inlet check valve is communicated in the second air inlet hole, a second air outlet check valve is communicated in the second air outlet hole, a second air outlet pipe is communicated at the second air outlet hole, a plurality of second through holes are formed in the bottom frame along the circumferential direction, and the second air outlet pipe is communicated with the second through holes.
2. The method for extracting the soy protein from the bean dregs as claimed in claim 1, wherein the method comprises the following steps: in the step B, surfactant with the concentration of 70-100ppm is synchronously added with the cellulase solution, and the weight ratio of the surfactant to the cellulase solution is 1: 5.
3. the method for extracting soy protein from bean dregs as claimed in claim 2, wherein: in the step C, the ultrasonic extraction conditions are as follows: the ultrasonic power is 500-800W, the temperature is 40-80 ℃, and the time is 20-30 minutes.
4. The method for extracting soy protein from bean dregs as claimed in claim 3, wherein: in the step A, the heating temperature is 50 ℃ and the heating time is 1 hour.
5. The method for extracting soy protein from bean dregs as claimed in claim 4, wherein: in the step B, the surfactant is a nonionic surfactant.
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| CN201811110329.8A CN109251233B (en) | 2018-09-21 | 2018-09-21 | Method for extracting bean protein from bean dregs |
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| CN201811110329.8A CN109251233B (en) | 2018-09-21 | 2018-09-21 | Method for extracting bean protein from bean dregs |
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| CN114009577A (en) * | 2021-11-24 | 2022-02-08 | 哈尔滨市曦驰生物科技有限责任公司 | Method for extracting bean protein from bean dregs |
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