CN107814830A - A kind of method of the heavy method production soybean protein isolate of high-temperature acid - Google Patents
A kind of method of the heavy method production soybean protein isolate of high-temperature acid Download PDFInfo
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- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
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Abstract
A kind of method of the heavy method production soybean protein isolate of high-temperature acid, comprises the following steps：(1) by raw material defatted soybean meal and 35 45 DEG C of water by weight 1:5 15 mixing, regulation pH value to 6.8 7.5, crush, sterilize, isolated a bean dregs and a soya-bean milk after stirring；(2) pH value of soya-bean milk obtained by step (1) is adjusted to 4.3 4.8, settled；(3) by flash liberation after the albumen suspension heating after sedimentation, then cool, isolated acid Solid phase protein slurry；(4) pH to 6.5 7.5 is adjusted after acid Solid phase protein slurry plus water mixing；(5) the feed liquid sterilization after being neutralized obtained by step (4), flash liberation, dried recovered are obtained into soybean protein isolate product.The method of the present invention can reduce protein content in whey wastewater, reduce the heavy loss of acid of albumen, improve the rate of recovery of soybean protein, reduce whey wastewater intractability, reduce the usage amount of aluminium polychloride, reduce environmental pollution and dangerous item station.
Description
Technical field
The present invention relates to a kind of extractive technique field of soybean protein isolate, more particularly to a kind of high-temperature acid to sink method production greatly
The method of beans protein isolate.
Background technology
Soybean protein is unique 9 kinds of essential amino acids containing needed by human body for reporting at present and content meets human body requirements
A kind of vegetable protein without cholesterol, be a kind of generally acknowledged complete protein, animal protein and cereal can be made up simultaneously
The defects of protein meal is present.But China resident peas protein intake is less, the intake of particularly soybean protein is only
5%, far below recommended amounts 20%.On the basis of the trophic function of soybean protein has been affirmed, for a long time, researchers are never
The research to soybean protein healthcare function was abandoned, blood fat, cholesterol is reduced experimental results demonstrate soybean protein has, improves
Insulin sensitivity and fat-reducing and other effects.
The result of study and data of a large amount of scientists are based on, 1999, FDA (FDA) was sent out
The cloth Health Claims of soybean protein, i.e., " edible 25g soybean proteins can reduce the wind of heart disease, angiocardiopathy daily
Danger." developed country such as the U.S., Europe, Japan actively promoting the industrialization process of soybean protein, as reply aging
One of state basic policy that society arrives, the quantity of its soy protein products occupies very important proportion in food industries at present,
And the development starting of China's soybean protein is relatively later, product category is few.Therefore, generally deposited for China national " inferior health "
With the irrational national conditions of dietary structure, revitalize soybean protein industry, to improve state human protein intake quality and it is horizontal and
Alleviating chronic diseases in China present situation occurred frequently has important meaning.
Soybean protein isolate is a kind of full price protide food additives produced using low temperature desolventizing Soybean Meal as raw material.Greatly
For protein content more than 90%, amino acid classes have nearly 20 kinds, and contain essential amino acid in beans protein isolate.It is sought
Support and enrich, be one of kinds of alternative animal protein few in number in vegetable protein without cholesterol.Simultaneously because soybean
Protein isolate excellent water retention property and gelling performance, make it be widely applied in varieties of food items, particularly ham sausage, injection
Barbecue, various Frozen Prepared Foods etc..The common production method of soybean protein isolate includes：Extraction, acid is heavy, neutralizes, sterilizes, spray
Mist is dried.Such as current soybean protein production technology scheme is：The protein extracting in low temperature soy meal is gone out by using light buck
Come after through being centrifugally separating to obtain an extraction soya-bean milk and one extraction bean dregs, one extraction bean dregs after washing it is isolated two extraction soya-bean milk, two
Extraction soya-bean milk and an extraction soya-bean milk obtain solid phase curdled milk through peracid is heavy, and solid phase curdled milk is spray-dried after neutralization；Comprise the following steps that：
A) extract：By raw material defatted soybean meal and water by weight 1:8-12 ratio mixing, regulation pH value to 6.5-
8.0, stirring；B) centrifuge：Extract solution in step a) is centrifuged, obtains bean dregs and soya-bean milk；C) acid is heavy：By beans
The pH value of breast is adjusted to 4.5-5.0, is settled；D) centrifuge：The heavy liquid of acid in step c) is centrifuged, obtained
Solid phase acidic protein precipitates；E) neutralize：The water of acidic protein precipitation and 3-5 times of weight is stirred, pH to 6.5-7.5 is adjusted, obtains
Feed liquid after to neutralization；F) dry：Feed liquid after neutralization is sterilized, is spray-dried after flash distillation, recovery obtains soybean protein isolate
Product.
Substantial amounts of whey wastewater is produced in the production process, high-quality small molecule soybean egg is contained in this part whey wastewater
In vain, it is impossible to recycle, wherein crude protein reaches 20% or so.And need to be located in this part whey wastewater industrial production
Reason could discharge after reaching respective standard.All it is to be removed again with biochemical method after using polyaluminium aluminum precipitation in the industry at present.
In addition, sodium hydroxide is used to adjust pH as pH adjusting agent to cause the albumen in low temperature soy meal in such scheme
Dissolution in the basic conditions, by the heavy addition hydrochloric acid isoelectric precipitation of acid, sodium chloride is generated in the process.And the chlorine of low concentration
The solubility of albumen can be improved by changing sodium, be unfavorable for isoelectric precipitation, and contain multiple protein composition in soybean protein, wait electricity
Point is pH4.0-5.8, wider range, and conventional isoelectric point is controlled between pH4.5-4.8.But within this range, part egg
It can not precipitate in vain, cause protein loss, the albumen of loss then needs to be removed by biochemical reaction after polyaluminium aluminum precipitation, this
Sample not only wastes protein resource, also increases sewage disposal difficulty.
The content of the invention
For this it is an object of the invention to provide a kind of method of the heavy method production soybean protein isolate of high-temperature acid, this method can
The protein content in whey wastewater is reduced, the heavy loss of acid of albumen is reduced, improves the rate of recovery of soybean protein, reduce whey wastewater
Intractability, the usage amount of aluminium polychloride is reduced, reduced environmental pollution and dangerous item station.
For the above-mentioned purpose, the present invention adopts the following technical scheme that：
A kind of method of the heavy method production soybean protein isolate of high-temperature acid, comprises the following steps：
(1) by raw material defatted soybean meal and 35-45 DEG C of water by weight 1:5-15 is mixed, regulation pH value to 6.8-7.5,
Crush, sterilize, isolated a bean dregs and a soya-bean milk after stirring；PH can be adjusted by adding sodium hydroxide, ammoniacal liquor etc.；
(2) pH value of soya-bean milk obtained by step (1) is adjusted to 4.3-4.8, settled；
(3) by flash liberation after the albumen suspension heating after sedimentation, then cool, isolated acid Solid phase protein
Slurry；
(4) pH to 6.5-7.5 is adjusted after acid Solid phase protein slurry plus water mixing；
(5) the feed liquid sterilization after being neutralized obtained by step (4), flash liberation, dried recovered are obtained into soybean protein isolate production
Product.
The present invention can reduce the protein content in whey wastewater by the cooperation of above-mentioned steps, improve protein recovery, subtract
The heavy loss of acid of few albumen, reduces sewage disposal difficulty, improves product yield.
Preferably, the weight of defatted soybean meal and water ratio is 1 in step (1):8-10.
Preferably, the time of stirring is more than 10min, preferably 15-45min.
Preferably, crushed using multistage Homogenizing pump or other wet pulverizing equipment, preferably entered using multistage Homogenizing pump
Row crushes.
Preferably, the material after crushing is delivered to holding vessel.
Preferably, sterilization is carried out using microwave, ultrasonic wave or microwave combined ultrasonic wave, sterilizing time 2-8min.
Preferably, storage 20-50min, preferably 30min before separation.
Preferably, a bean dregs after separation obtain secondary bean dregs and secondary soya-bean milk after washing separates, by a beans
Newborn and secondary soya-bean milk is mixed into next step.
Preferably, addition Tween 80, the 0.1-1 ‰ for being preferably added to solidity thing content are told before sedimentation in step (2)
Temperature 80, solidity thing are the quality after soya-bean milk drying.The purpose for adding Tween 80 is to destroy albumen using molecules surfactant
Hydrated sheath, so as to have the function that to promote albumen aggregation.
Preferably, the temperature of heating is 65-95 DEG C in step (3).
Preferably, it is cooled to 40-45 DEG C.
Preferably, the weight ratio of acid Solid phase protein slurry and water is 1 in step (4):2-10, preferably 1:3-5.
Preferably, it is a kind of in edible industrial alkaline processing aid, sodium hydroxide or potassium hydroxide by adding to adjust pH
Or several progress, it is preferably added to eat one or more of in industrial alkaline processing aid.
Preferably, it is alkali face and/or sodium bicarbonate to eat industrial alkaline processing aid.
Preferably, the temperature of sterilization is 130-145 DEG C in step (5), the time is the 3-8 seconds.
Preferably, flash liberation can be that multistage flash evaporation deaerates.
Preferably, dry using spray drying.
Separation in the present invention can be to centrifuge, and other separate modes, such as plate-frame filtering, press filtration etc. also can be selected.
Preferably, the method for the present invention comprises the following steps：
A) extract：By raw material defatted soybean meal and water by weight 1:8-10 ratio mixing, adds sodium hydroxide regulation
PH value 35-45 DEG C of water temperature, after stirring 15min, is crushed using multistage Homogenizing pump to 6.8-7.5 and is delivered to holding vessel, utilize microwave
Sterilization processing 2-5min, storage 30min etc. is to be separated；
B) centrifuge：Extract solution in step a) is centrifuged, obtains a bean dregs and a soya-bean milk, once
Bean dregs obtain secondary bean dregs and secondary soya-bean milk after washing, and a soya-bean milk and secondary soya-bean milk are mixed；
C) acid is heavy：The pH value of soya-bean milk and the mixture of secondary soya-bean milk is adjusted to 4.3-4.8, settled；Sedimentation
The Tween 80s of 0.1-1 ‰ of optional addition solidity thing (quality after soya-bean milk drying) content before.
D) albumen suspension after acid is heavy is by being heated to 65-95 DEG C, rear flash liberation, and is cooled to 40-45 DEG C；
E) centrifuge：The heavy liquid of acid in step d) is centrifuged, obtains acid Solid phase protein slurry；
F) neutralize：Acid Solid phase protein slurry and the water of 3-5 times of weight are stirred, adds and eats industrial alkaline processing aid
Adjust pH to 6.5-7.5；
G) sterilize：The 130-145 DEG C of sterilization 3-8 second of feed liquid will be neutralized；Flash liberation after sterilization；
H) dry：Feed liquid after flash liberation, spray drying, recovery obtain soybean protein isolate product.
The present invention has the advantages that：
1st, the rate of recovery of albumen is improved into 1-5%；
2nd, the COD value of whey wastewater is reduced into 2000-4000；
3rd, total plate count in soybean protein is reduced into 10-20%；
4th, albumen retentiveness is improved into 10-15%；
5th, the beany flavor of protein product is reduced, improves product quality.
Embodiment
For ease of understanding the present invention, it is as follows that the present invention enumerates embodiment.Those skilled in the art are it will be clearly understood that the implementation
Example is used only for help and understands the present invention, is not construed as the concrete restriction to the present invention.
Embodiment 1
A kind of method of the heavy method production soybean protein isolate of high-temperature acid, comprises the following steps：
A) extract：By raw material defatted soybean meal and water by weight 1:8 ratio mixing, add sodium hydroxide regulation pH value
To 6.8,45 DEG C of water temperature, after stirring 15min, crushed using multistage Homogenizing pump and be delivered to holding vessel, handled using microwave disinfection
5min, storage 30min etc. is to be separated；
B) centrifuge：Extract solution in step a) is centrifuged, obtains a bean dregs and a soya-bean milk, once
Bean dregs obtain secondary bean dregs and secondary soya-bean milk after washing, and a soya-bean milk and secondary soya-bean milk are mixed；
C) acid is heavy：The pH value of soya-bean milk and the mixture of secondary soya-bean milk is adjusted to 4.3, settled；Before sedimentation
Add 0.2 ‰ Tween 80s of solidity thing content；
D) albumen suspension after acid is heavy is by being heated to 70 DEG C, rear flash liberation, and is cooled to 45 DEG C；
E) centrifuge：The heavy liquid of acid in step d) is centrifuged, obtains acid Solid phase protein slurry；
F) neutralize：Acid Solid phase protein slurry and the water of 3 times of weight are stirred, edible industrial alkaline processing aid is added and adjusts
Save pH to 7.5；
G) sterilize：The 130-145 DEG C of sterilization 3-8 second of feed liquid will be neutralized；Flash liberation after sterilization；
H) dry：Feed liquid after flash liberation, spray drying, recovery obtain soybean protein isolate product.
Compare after testing and draw, the existing skill of the technique of the more existing alkali extraction-acid precipitation extraction soybean protein isolate of the embodiment
Art method improves the rate of recovery 1.5-2% of albumen；Reduce COD (COD) the values 2000-2500mg/L of whey wastewater；Drop
Total plate count 10-12% in low soybean protein；Improve albumen retentiveness 10-12%；The beany flavor of protein product is reduced, is improved
Product quality.
Embodiment 2
A kind of method of the heavy method production soybean protein isolate of high-temperature acid, comprises the following steps：
A) extract：By raw material defatted soybean meal and water by weight 1:10 ratio mixing, add sodium hydroxide regulation pH
Value 35 DEG C of water temperature, after stirring 15min, is crushed using multistage Homogenizing pump to 7.5 and is delivered to holding vessel, handled using microwave disinfection
2min, storage 30min etc. is to be separated；
B) centrifuge：Extract solution in step a) is centrifuged, obtains a bean dregs and a soya-bean milk, once
Bean dregs obtain secondary bean dregs and secondary soya-bean milk after washing, and a soya-bean milk and secondary soya-bean milk are mixed；
C) acid is heavy：The pH value of soya-bean milk and the mixture of secondary soya-bean milk is adjusted to 4.8, settled；Before sedimentation
Add 0.3 ‰ Tween 80s of solidity thing content；
D) albumen suspension after acid is heavy is by being heated to 75 DEG C, rear flash liberation, and is cooled to 40 DEG C；
E) centrifuge：The heavy liquid of acid in step d) is centrifuged, obtains acid Solid phase protein slurry；
F) neutralize：Acid Solid phase protein slurry and the water of 5 times of weight are stirred, edible industrial alkaline processing aid is added and adjusts
Save pH to 7.5；
G) sterilize：The 130-145 DEG C of sterilization 3-8 second of feed liquid will be neutralized；Flash liberation after sterilization；
H) dry：Feed liquid after flash liberation, spray drying, recovery obtain soybean protein isolate product.
Compare after testing and draw, the existing skill of the technique of the more existing alkali extraction-acid precipitation extraction soybean protein isolate of the embodiment
Art method improves the rate of recovery 2-4% of albumen；Reduce the COD value 2500-3500mg/L of whey wastewater；Reduce bacterium in soybean protein
Fall total 15-18%；Improve albumen retentiveness 10-12%；The beany flavor of protein product is reduced, improves product quality.
Embodiment 3
A kind of method of the heavy method production soybean protein isolate of high-temperature acid, comprises the following steps：
A) extract：By raw material defatted soybean meal and water by weight 1:5 ratio mixing, add sodium hydroxide regulation pH value
To 7.0,40 DEG C of water temperature, after stirring 10min, crushed using multistage Homogenizing pump and be delivered to holding vessel, handled using microwave disinfection
3min, storage 20min etc. is to be separated；
B) centrifuge：Extract solution in step a) is centrifuged, obtains a bean dregs and a soya-bean milk, once
Bean dregs obtain secondary bean dregs and secondary soya-bean milk after washing, and a soya-bean milk and secondary soya-bean milk are mixed；
C) acid is heavy：The pH value of soya-bean milk and the mixture of secondary soya-bean milk is adjusted to 4.5, settled；Before sedimentation
Add 0.1 ‰ Tween 80s of solidity thing content；
D) albumen suspension after acid is heavy is by being heated to 80 DEG C, rear flash liberation, and is cooled to 42 DEG C；
E) centrifuge：The heavy liquid of acid in step d) is centrifuged, obtains acid Solid phase protein slurry；
F) neutralize：Acid Solid phase protein slurry and the water of 4 times of weight are stirred, edible industrial alkaline processing aid is added and adjusts
Save pH to 7.0；
G) sterilize：The 130-145 DEG C of sterilization 3-8 second of feed liquid will be neutralized；Flash liberation after sterilization；
H) dry：Feed liquid after flash liberation, spray drying, recovery obtain soybean protein isolate product.
Compare after testing and draw, the existing skill of the technique of the more existing alkali extraction-acid precipitation extraction soybean protein isolate of the embodiment
Art method improves the rate of recovery 3-5% of albumen；Reduce the COD value 2500-3500 of whey wastewater；It is total to reduce bacterium colony in soybean protein
Number 15-20%；Improve albumen retentiveness 14-15%；The beany flavor of protein product is reduced, improves product quality.
Embodiment 4
A kind of method of the heavy method production soybean protein isolate of high-temperature acid, comprises the following steps：
A) extract：By raw material defatted soybean meal and water by weight 1:15 ratio mixing, add sodium hydroxide regulation pH
Value 38 DEG C of water temperature, after stirring 25min, is crushed using multistage Homogenizing pump to 7.3 and is delivered to holding vessel, handled using microwave disinfection
5min, storage 50min etc. is to be separated；
B) centrifuge：Extract solution in step a) is centrifuged, obtains a bean dregs and a soya-bean milk, once
Bean dregs obtain secondary bean dregs and secondary soya-bean milk after washing, and a soya-bean milk and secondary soya-bean milk are mixed；
C) acid is heavy：The pH value of soya-bean milk and the mixture of secondary soya-bean milk is adjusted to 4.7, settled；Before sedimentation
Add 0.5 ‰ Tween 80s of solidity thing content；
D) albumen suspension after acid is heavy is by being heated to 77 DEG C, rear flash liberation, and is cooled to 44 DEG C；
E) centrifuge：The heavy liquid of acid in step d) is centrifuged, obtains acid Solid phase protein slurry；
F) neutralize：Acid Solid phase protein slurry and the water of 4 times of weight are stirred, edible industrial alkaline processing aid is added and adjusts
Save pH to 6.8；
G) sterilize：The 130-145 DEG C of sterilization 3-8 second of feed liquid will be neutralized；Flash liberation after sterilization；
H) dry：Feed liquid after flash liberation, spray drying, recovery obtain soybean protein isolate product.
Compare after testing and draw, the existing skill of the technique of the more existing alkali extraction-acid precipitation extraction soybean protein isolate of the embodiment
Art method improves the rate of recovery 3-5% of albumen；Reduce the COD value 2500-3500mg/L of whey wastewater；Reduce bacterium in soybean protein
Fall total 15-20%；Improve albumen retentiveness 12-14%；The beany flavor of protein product is reduced, improves product quality.
Embodiment 5
A kind of method of the heavy method production soybean protein isolate of high-temperature acid, comprises the following steps：
A) extract：By raw material defatted soybean meal and water by weight 1:15 ratio mixing, add sodium hydroxide regulation pH
Value 38 DEG C of water temperature, after stirring 25min, is crushed using multistage Homogenizing pump to 7.3 and is delivered to holding vessel, handled using microwave disinfection
5min, storage 50min etc. is to be separated；
B) centrifuge：Extract solution in step a) is centrifuged, obtains a bean dregs and a soya-bean milk, once
Bean dregs obtain secondary bean dregs and secondary soya-bean milk after washing, and a soya-bean milk and secondary soya-bean milk are mixed；
C) acid is heavy：The pH value of soya-bean milk and the mixture of secondary soya-bean milk is adjusted to 4.7, settled；Before sedimentation
Add 1 ‰ Tween 80s of solidity thing content；
D) albumen suspension after acid is heavy is by being heated to 77 DEG C, rear flash liberation, and is cooled to 44 DEG C；
E) centrifuge：The heavy liquid of acid in step d) is centrifuged, obtains acid Solid phase protein slurry；
F) neutralize：Acid Solid phase protein slurry and the water of 4 times of weight are stirred, edible industrial alkaline processing aid is added and adjusts
Save pH to 6.8；
G) sterilize：The 130-145 DEG C of sterilization 3-8 second of feed liquid will be neutralized；Flash liberation after sterilization；
H) dry：Feed liquid after flash liberation, spray drying, recovery obtain soybean protein isolate product.
Compare after testing and draw, the existing skill of the technique of the more existing alkali extraction-acid precipitation extraction soybean protein isolate of the embodiment
Art method improves the rate of recovery 4-5% of albumen；Reduce the COD value 3000-3500mg/L of whey wastewater；Reduce bacterium in soybean protein
Fall total 17-20%；Improve albumen retentiveness 13-14%；The beany flavor of protein product is reduced, improves product quality.
Embodiment 6
A) extract：By raw material defatted soybean meal and water by weight 1:8 ratio mixing, add sodium hydroxide regulation pH value
To 6.8,45 DEG C of water temperature, after stirring 15min, crushed using multistage Homogenizing pump and be delivered to holding vessel, handled using microwave disinfection
5min, storage 30min etc. is to be separated；
B) centrifuge：Extract solution in step a) is centrifuged, obtains a bean dregs and a soya-bean milk, once
Bean dregs obtain secondary bean dregs and secondary soya-bean milk after washing, and a soya-bean milk and secondary soya-bean milk are mixed；
C) acid is heavy：The pH value of soya-bean milk and the mixture of secondary soya-bean milk is adjusted to 4.3, settled；
D) albumen suspension after acid is heavy is by being heated to 70 DEG C, rear flash liberation, and is cooled to 45 DEG C；
E) centrifuge：The heavy liquid of acid in step d) is centrifuged, obtains acid Solid phase protein slurry；
F) neutralize：Acid Solid phase protein slurry and the water of 3 times of weight are stirred, edible industrial alkaline processing aid is added and adjusts
Save pH to 7.5；
G) sterilize：The 130-145 DEG C of sterilization 3-8 second of feed liquid will be neutralized；Flash liberation after sterilization；
H) dry：Feed liquid after flash liberation, spray drying, recovery obtain soybean protein isolate product.
Compare after testing and draw, the existing skill of the technique of the more existing alkali extraction-acid precipitation extraction soybean protein isolate of the embodiment
Art method improves the rate of recovery 1-1.5% of albumen；Reduce COD (COD) the values 1800-2000mg/L of whey wastewater；Drop
Total plate count 10-12% in low soybean protein；Improve albumen retentiveness 10-12%；The beany flavor of protein product is reduced, is improved
Product quality.
The COD, total plate count and retentiveness result of above-described embodiment see the table below shown in 1.
Comparative example 1
It is same as Example 1, except the heavy rear directly progress e) centrifugation of step c) acid.
As a result see the table below shown in 1.
As a result see the table below shown in 1.Comparing result is, by heating the COD contents reduced in waste water, to reduce sewage disposal
Difficulty, it is cost-effective, protein recovery is improved, the retentiveness of albumen improves, and improves the application quality of albumen.
Table 1
| COD(mg/L) | Total plate count (cfu/g) | Retentiveness (mL/g) | |
| Embodiment 1 | 20800 | 8900 | 6.1 |
| Embodiment 2 | 20000 | 8600 | 6.0 |
| Embodiment 3 | 19800 | 8500 | 6.2 |
| Embodiment 4 | 20100 | 8300 | 6.1 |
| Embodiment 5 | 19000 | 8100 | 6.0 |
| Embodiment 6 | 20600 | 8800 | 6.0 |
| Comparative example 1 | 23000 | 10500 | 5.6 |
Retentiveness test experience is verified
1.00g soybean protein isolate samples are weighed, are placed in 50mL centrifuge tubes, add distilled water 10mL.Treat that it is all soaked
Afterwards, 5min is stirred with glass bar, protein suspending liquid is uniformly dispersed, 25min is centrifuged under 2500r/min.Reading is not separated
The milliliter number reading v of the water (water of precipitation) of absorbing proteins.The average value of 3 times is taken, retentiveness is calculated as follows：
COD, total plate count, protein recovery assay method are tested according to existing common method.
Obviously, above-described embodiment is only intended to clearly illustrate example, and is not the restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or
Among changing still in the protection domain of the invention.
Claims (10)
1. a kind of method of the heavy method production soybean protein isolate of high-temperature acid, comprises the following steps：
(1) by raw material defatted soybean meal and 35-45 DEG C of water by weight 1:5-15 is mixed, regulation pH value to 6.8-7.5, stirring
Crush, sterilize, isolated a bean dregs and a soya-bean milk afterwards；
(2) pH value of soya-bean milk obtained by step (1) is adjusted to 4.3-4.8, settled；
(3) by flash liberation after the albumen suspension heating after sedimentation, then cool, isolated acid Solid phase protein slurry；
(4) pH to 6.5-7.5 is adjusted after acid Solid phase protein slurry plus water mixing；
(5) the feed liquid sterilization after being neutralized obtained by step (4), flash liberation, dried recovered are obtained into soybean protein isolate product.
2. according to the method for claim 1, it is characterised in that the weight of defatted soybean meal and water ratio is in step (1)
1:8-10。
3. method according to claim 1 or 2, it is characterised in that the time of stirring is more than 10min in step (1), excellent
Elect 15-45min as.
4. according to the method described in claim any one of 1-3, it is characterised in that in step (1) using multistage Homogenizing pump or its
He is crushed wet pulverizing equipment, is preferably crushed using multistage Homogenizing pump；
Preferably, the material after crushing is delivered to holding vessel.
5. according to the method described in claim any one of 1-4, it is characterised in that sterilization utilizes microwave, ultrasonic wave in step (1)
Or microwave combined ultrasonic wave is carried out, sterilizing time 2-8min；
Preferably, storage 20-50min, preferably 30min before separation.
6. according to the method described in claim any one of 1-5, it is characterised in that the bean dregs warp after being separated in step (1)
Secondary bean dregs and secondary soya-bean milk are obtained after crossing washing separation, a soya-bean milk and secondary soya-bean milk are mixed into next step.
7. according to the method described in claim any one of 1-6, it is characterised in that addition Tween 80 before sedimentation in step (2),
It is preferably added to the 0.1-1 ‰ of solidity thing content Tween 80.
8. according to the method described in claim any one of 1-7, it is characterised in that the temperature of heating is 65-95 in step (3)
℃；
Preferably, it is cooled to 40-45 DEG C.
9. according to the method described in claim any one of 1-8, it is characterised in that acid Solid phase protein slurry and water in step (4)
Weight ratio be 1:2-10, preferably 1:3-5；
Preferably, it is a kind of or several in edible industrial alkaline processing aid, sodium hydroxide or potassium hydroxide by adding to adjust pH
Kind is carried out, and is preferably added to eat one or more of in industrial alkaline processing aid；
Preferably, it is alkali face and/or sodium bicarbonate to eat industrial alkaline processing aid.
10. according to the method described in claim any one of 1-9, it is characterised in that the temperature of sterilization is 130- in step (5)
145 DEG C, the time is the 3-8 seconds；
Preferably, flash liberation deaerates for multistage flash evaporation；
Preferably, dry using spray drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711086674.8A CN107814830A (en) | 2017-11-07 | 2017-11-07 | A kind of method of the heavy method production soybean protein isolate of high-temperature acid |
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| CN108925743A (en) * | 2018-08-21 | 2018-12-04 | 克东禹王大豆蛋白食品有限公司 | A kind of easy preparation method for absorbing soyabean protein powder |
| CN108935917A (en) * | 2018-08-23 | 2018-12-07 | 克东禹王大豆蛋白食品有限公司 | A kind of instant soluble soybean albumen powder and preparation method thereof |
| CN109170125A (en) * | 2018-09-29 | 2019-01-11 | 山东禹王生态食业有限公司 | A kind of preparation method of high fluidity soybean protein isolate |
| CN109251233A (en) * | 2018-09-21 | 2019-01-22 | 贵州省三好食品开发有限公司 | The method of legumin is extracted in a kind of bean dregs |
| CN112753842A (en) * | 2020-12-29 | 2021-05-07 | 临邑禹王植物蛋白有限公司 | High-yield starch and protein extraction method |
| CN113455581A (en) * | 2021-06-28 | 2021-10-01 | 临邑禹王植物蛋白有限公司 | Production method of pea-sesame composite plant protein |
| CN113647505A (en) * | 2021-08-26 | 2021-11-16 | 汤臣倍健股份有限公司 | Instant soybean protein powder and preparation method thereof |
| CN113768030A (en) * | 2021-09-14 | 2021-12-10 | 临邑禹王植物蛋白有限公司 | Production method of pea protein with low yellow value |
| CN115251310A (en) * | 2022-08-01 | 2022-11-01 | 山东御馨生物科技有限公司 | High-stability low-fat soybean milk powder and preparation method thereof |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108925743A (en) * | 2018-08-21 | 2018-12-04 | 克东禹王大豆蛋白食品有限公司 | A kind of easy preparation method for absorbing soyabean protein powder |
| CN108935917A (en) * | 2018-08-23 | 2018-12-07 | 克东禹王大豆蛋白食品有限公司 | A kind of instant soluble soybean albumen powder and preparation method thereof |
| CN109251233A (en) * | 2018-09-21 | 2019-01-22 | 贵州省三好食品开发有限公司 | The method of legumin is extracted in a kind of bean dregs |
| CN109251233B (en) * | 2018-09-21 | 2021-11-12 | 贵州省三好食品开发有限公司 | Method for extracting bean protein from bean dregs |
| CN109170125A (en) * | 2018-09-29 | 2019-01-11 | 山东禹王生态食业有限公司 | A kind of preparation method of high fluidity soybean protein isolate |
| CN109170125B (en) * | 2018-09-29 | 2022-03-22 | 山东禹王生态食业有限公司 | Preparation method of high-fluidity isolated soy protein |
| CN112753842A (en) * | 2020-12-29 | 2021-05-07 | 临邑禹王植物蛋白有限公司 | High-yield starch and protein extraction method |
| CN113455581A (en) * | 2021-06-28 | 2021-10-01 | 临邑禹王植物蛋白有限公司 | Production method of pea-sesame composite plant protein |
| CN113647505A (en) * | 2021-08-26 | 2021-11-16 | 汤臣倍健股份有限公司 | Instant soybean protein powder and preparation method thereof |
| CN113647505B (en) * | 2021-08-26 | 2024-04-05 | 汤臣倍健股份有限公司 | Instant soybean protein powder and preparation method thereof |
| CN113768030A (en) * | 2021-09-14 | 2021-12-10 | 临邑禹王植物蛋白有限公司 | Production method of pea protein with low yellow value |
| CN115251310A (en) * | 2022-08-01 | 2022-11-01 | 山东御馨生物科技有限公司 | High-stability low-fat soybean milk powder and preparation method thereof |
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