CN108935917A - A kind of instant soluble soybean albumen powder and preparation method thereof - Google Patents
A kind of instant soluble soybean albumen powder and preparation method thereof Download PDFInfo
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- CN108935917A CN108935917A CN201810964282.5A CN201810964282A CN108935917A CN 108935917 A CN108935917 A CN 108935917A CN 201810964282 A CN201810964282 A CN 201810964282A CN 108935917 A CN108935917 A CN 108935917A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/16—Vegetable proteins from soybean
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Abstract
The present invention provides a kind of instant soluble soybean albumen powders and preparation method thereof, it is prepared via a method which: extraction, separation, acid is heavy, washing, neutralize, sterilization, dry, micronization, the soyabean protein powder quality obtained using this method is stable, it is rapid to dissolve, it is suitble to various people edible, is a kind of health-care good product.
Description
Technical field
The present invention relates to plant soybean extraction processes, more specifically to a kind of instant soluble soybean albumen powder and its preparation
Method.
Background technique
Soybean protein is a kind of vegetable protein, and amino acid composition is close with milk protein, except methionine is slightly lower
Outside, remaining essential amino acids content is relatively abundant, is vegetal complete protein.It, can be with animal protein on nutritive value
It is equivalent, human amino acid is also closest on gene structure, so being the phytoprotein of most nutrition.Soybean protein has
The incomparable advantage of animal protein, although soybean protein methionine is few, however it is cholesterol-free, its distinctive physiology is living
Substance --- isoflavones has the function of norcholesterol to property.
In October, 1999, United States Food and Drag Administration suggestion " edible 25g soybean protein daily, it is possible to reduce coronary disease
The risk of disease ", this is the 11st Health Claims of FDA since the dawn of human civilization.
Currently, scientists have been accepted, soybean in heart health, prevent osteoporosis and alleviate Woman climacteric symptom
Etc. it is all very useful.
Protein exists in water with dispersed (colloidal attitude) as organic macromolecule molecular compound, therefore dissolubility
It is poor, using effect is influenced, in view of this, a kind of quality is needed to stablize in the prior art, dissolves rapid soyabean protein powder.
Summary of the invention
For the deficiencies in the prior art, the object of the present invention is to provide a kind of quality stabilization, dissolution are rapidly big
Legumin powder, while preparation method being provided.
One aspect of the present invention provides a kind of instant soluble soybean albumen powder, which is characterized in that jitter time was led to less than 15 seconds
Cross following steps preparation:
(1) extract: using soybean low temperature soy meal as raw material, being put into extractor by 1(soybean low temperature soy meal): 8 ~ 9(water)
Water is added in ratio, and water temperature control is 30 ~ 35 DEG C, addition aqueous slkali adjusting pH value to 8.5 ~ 9.0, stirring, and the albumen in dregs of beans is molten
In Xie Yushui；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) optionally, using the isolated bean dregs of step (2) as raw material, it is put into extractor by 1(bean dregs): 4 ~ 5(water)
Ratio water is added, water temperature control is 30 ~ 35 DEG C, aqueous slkali is added and adjusts pH value to 8.5 ~ 9.0, stirring, the albumen in bean dregs
It is dissolved in the water；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: acid is added, adjusts the pH to 4.4 ~ 4.6 of the water containing albumen in the heavy tank of acid；Stirring 3 ~ 5 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) optionally, washing: by 1(curdled milk): 3 ~ 5(weight ratio) add water to enter in curd-containing canisters, it stirs, separation, protein liquid (abbreviation:
Curdled milk) arrive curd-containing canisters 2；
(7) it neutralizes: aqueous slkali is added in the curd-containing canisters 1 in step (4) or the curd-containing canisters 2 in step (5), adjusts PH and is adjusted to
6.5~7.5；
(8) sterilize, be dry: the lysate of step (6) is sterilized, after lysate is dry；
(9) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist；
In the step (1), the aqueous slkali that the preferred pH of the aqueous slkali is 9.5 ~ 10.5, more preferable pH is 9.5 ~ 10.5
Sodium carbonate liquor, sodium hydroxide solution, potassium hydroxide solution；
In the step (1), soybean low temperature soy meal, CP(butt) it is not less than 55%, NSI(nitrogen soluble index) it is not less than 85%；
In the step (1), stirring rate is 500 ~ 1000 turns/min(minutes), mixing time is 60 ~ 120min；
The step (7), the aqueous slkali that the preferred pH of the aqueous slkali is 9.5 ~ 10.5, the carbon that more preferable pH is 9.5 ~ 10.5
Acid sodium solution, sodium hydroxide solution, potassium hydroxide solution；
The step (8), the drying can be high and dry for constant-pressure and high-temperature, or decompression is high and dry, or freezing
It is dry, preferably freeze drying.
Another aspect of the present invention provides a kind of preparation method of above-mentioned instant protein powder comprising following steps:
(1) extract: using soybean low temperature soy meal as raw material, being put into extractor by 1(soybean low temperature soy meal): 8 ~ 9(water)
Water is added in ratio, and water temperature control is 30 ~ 35 DEG C, addition aqueous slkali adjusting pH value to 8.5 ~ 9.0, stirring, and the albumen in dregs of beans is molten
In Xie Yushui；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) optionally, using the isolated bean dregs of step (2) as raw material, it is put into extractor by 1(bean dregs): 4 ~ 5(water)
Ratio water is added, water temperature control is 30 ~ 35 DEG C, aqueous slkali is added and adjusts pH value to 8.5 ~ 9.0, stirring, the albumen in bean dregs
It is dissolved in the water；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: acid is added, adjusts the pH to 4.4 ~ 4.6 of the water containing albumen in the heavy tank of acid；Stirring 3 ~ 5 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) optionally, washing: by 1(curdled milk): 3 ~ 5(weight ratio) add water to enter in curd-containing canisters, it stirs, separation, protein liquid (abbreviation:
Curdled milk) arrive curd-containing canisters 2；
(7) it neutralizes: aqueous slkali is added in the curd-containing canisters 1 in step (4) or the curd-containing canisters 2 in step (5), adjusts PH and is adjusted to
6.5~7.5；
(8) sterilize, be dry: the lysate of step (6) is sterilized, after lysate is dry；
(9) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist；
In the step (1), the aqueous slkali that the preferred pH of the aqueous slkali is 9.5 ~ 10.5, more preferable pH is 9.5 ~ 10.5
Sodium carbonate liquor, sodium hydroxide solution, potassium hydroxide solution；
In the step (1), soybean low temperature soy meal, CP(butt) it is not less than 55%, NSI(nitrogen soluble index) it is not less than 85%；
In the step (1), the defoaming agent of low temperature soy meal weight 0.5% ~ 1% can also be added, the defoaming agent can be with two
First silicone oil or Simethicone, preferably Simethicone；
In the step (1), the stabilizer of low temperature soy meal weight 0.5% ~ 1% can also be added, the anti-stabilizer is coke
Sodium sulfite；
In the step (1), stirring rate is 500 ~ 1000 turns/min(minutes), mixing time is 60 ~ 120min.
In the step (4), the acid is hydrochloric acid, the citric acid of pH value 3 ~ 5.
The step (4), can two step acid sink method, specifically: (4) acid is heavy: being added in the heavy tank of acid adjustment acid and contains egg
The PH to 5.1 ~ 5.5 of white water is slowly stirred 1 ~ 3 hour, continuously adds acid for adjusting pH to 4.4 ~ 4.6, it is small to be slowly stirred 2 ~ 4
When, described is slowly stirred as 6 ~ 8 turns/min.
The step (7), the aqueous slkali that the preferred pH of the aqueous slkali is 9.5 ~ 10.5, more preferable pH are 9.5 ~ 10.5
Sodium carbonate liquor, sodium hydroxide solution, potassium hydroxide solution.
The step (8), the drying can be high and dry for constant-pressure and high-temperature, or decompression is high and dry, or
Freeze-drying, preferably freeze drying.
The step (8), the partial size are no more than 50 microns of micro mist, and preferable particle size is micro- no more than 400 microns
Powder, more preferably no more than 320 microns of micro mist.
Soyabean protein powder provided by the invention dissolves, jitter time be lower than 15 seconds, separation time up to 10 minutes or so,
It is a kind of health-care good product.
Specific embodiment
The invention discloses the preparation method of instant soluble soybean albumen powder, those skilled in the art can use for reference of the invention interior
Hold, is suitably modified prescription with when realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications are to ability
It is it will be apparent that they are considered as being included in the scope of the present invention for field technique personnel.Application of the invention has been led to
Preferred embodiment is crossed to be described, related personnel obviously can not depart from the content of present invention, in spirit and scope to this paper institute
The methods and applications stated are modified or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
Next by the following examples the present invention is further explained, but embodiment does not do any restriction to the present invention.
Test method in following embodiments is unless otherwise specified conventional method, original as used in the following examples
Material, reagent material etc. are commercially available products unless otherwise specified.
The index of soyabean protein powder involved in comparative example and embodiment:
Dispersibility: time≤15S, separation time >=3min
Physical and chemical index: moisture≤5.0%；CP(butt) >=90.0% ash content≤4.7%
Coloration L >=81.0 density >=340g/L
Embodiment 1: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) extract: using soybean low temperature soy meal as raw material, be put into extractor by 1(soybean low temperature soy meal): 9(water) ratio
Water is added in example, and water temperature control is 35 DEG C, and sodium hydroxide solution (pH=9.5) are added and adjust pH value to 8.5, control stirring rate
1000 turns/min, 60min is stirred, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) acid is heavy: it is added hydrochloric acid (pH=3), the pH to 4.4 of the water containing albumen in the heavy tank of adjustment acid, 6 turns of stirring rate of control/
Min is stirred 3 hours；
(4) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(5) neutralize: sodium hydroxide solution (pH=9.5) are added in the curd-containing canisters in step (4), adjust PH and are adjusted to 6.5；
(6) sterilize, be dry: the lysate of step (5) is sterilized, after lysate is freeze-dried；
(7) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist.
The soyabean protein powder obtained to implementation 1 detects, as a result as follows:
Embodiment 2: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) extract: using soybean low temperature soy meal as raw material, be put into extractor by 1(soybean low temperature soy meal): 8(water) ratio
Water is added in example, and water temperature control is 30 DEG C, and sodium hydroxide solution (pH=10.5) are added and adjust pH value to 9.0, control stirring rate
500 turns/min, 120min is stirred, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) acid is heavy: it is added hydrochloric acid (pH=5), the pH to 4.6 of the water containing albumen in the heavy tank of adjustment acid, 8 turns of stirring rate of control/
Min is stirred 5 hours；
(4) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(5) neutralize: sodium hydroxide solution (pH=10.5) are added in the curd-containing canisters 1 in step (4), adjust PH and are adjusted to 7.5；
(6) sterilize, be dry: the lysate of step (5) is sterilized, after lysate is freeze-dried；
(7) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist.
The soyabean protein powder obtained to implementation 2 detects, as a result as follows:
Embodiment 3: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) extract: using soybean low temperature soy meal as raw material, being put into extractor by 1(soybean low temperature soy meal): 8.2(water)
Water is added in ratio, and water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, control stirring rate 700
Turn/min, stirs 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) acid is heavy: it is added hydrochloric acid (pH=4), the pH to 4.5 of the water containing albumen in the heavy tank of adjustment acid, 6 turns of stirring rate of control/
Min is stirred 4 hours；
(4) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(5) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 1 in step (4), adjust PH and are adjusted to 7.0；
(6) sterilize, be dry: the lysate of step (5) is sterilized, after lysate is freeze-dried；
(7) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist.
The soyabean protein powder obtained to implementation 3 detects, as a result as follows:
Embodiment 4: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) extract: using soybean low temperature soy meal as raw material, being put into extractor by 1(soybean low temperature soy meal): 8.2(water)
Water is added in ratio, and water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, control stirring rate 700
Turn/min, stirs 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) using the isolated bean dregs of step (2) as raw material, be put into extractor in 1(bean dregs): 5(water) ratio add
Enter water, water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, stir, the protein dissolution in bean dregs
Yu Shuizhong；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: it is added hydrochloric acid (pH=4), the pH to 4.5 of the water containing albumen in the heavy tank of adjustment acid, 7 turns of stirring rate of control/
Min is stirred 4 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 1 in step (4), adjust PH and are adjusted to 7.0；
(7) sterilize, be dry: the lysate of step (5) is sterilized, after lysate is freeze-dried；
(8) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist.
The soyabean protein powder obtained to implementation 4 detects, as a result as follows:
Embodiment 5: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) extract: using soybean low temperature soy meal as raw material, being put into extractor by 1(soybean low temperature soy meal): 8.2(water)
Water is added in ratio, and water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, control stirring rate 700
Turn/min, stirs 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) using the isolated bean dregs of step (2) as raw material, be put into extractor in 1(bean dregs): 5(water) ratio add
Enter water, water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, stir, the protein dissolution in bean dregs
Yu Shuizhong；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: acid is heavy: being added hydrochloric acid (pH=4), adjusts the pH to 4.5 of the water containing albumen in the heavy tank of acid, control stirring speed
8 turns/min of rate is stirred 4 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) washing: by 1(curdled milk): 4(weight ratio) add water to enter in curd-containing canisters, it stirs, separation, protein liquid (referred to as: curdled milk), which arrives, to coagulate
Newborn tank 2；
(7) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 2 in step (6), adjust PH and are adjusted to 7.0；
(8) sterilize, be dry: the lysate of step (7) is sterilized, after lysate is freeze-dried；
(9) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist.
The soyabean protein powder obtained to implementation 5 detects, as a result as follows:
Embodiment 6: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) extract: using soybean low temperature soy meal as raw material, be put into extractor by 1(soybean low temperature soy meal): 9(water) ratio
Water is added in example, and water temperature control is 35 DEG C, and sodium hydroxide solution (pH=9.5) are added and adjust pH value to 8.5, control stirring rate
1000 turns/min, 60min is stirred, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) acid is heavy: it is added hydrochloric acid (pH=3), the PH to 5.1 of the water containing albumen in the heavy tank of adjustment acid, 6 turns of stirring rate of control/
Min is stirred 1 hour, is continuously added hydrochloric acid (pH=3) and is adjusted pH to 4.4, control 6 turns/min of stirring rate, stirs 4 hours；
(4) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(5) neutralize: sodium hydroxide solution (pH=9.5) are added in the curd-containing canisters in step (4), adjust PH and are adjusted to 6.5；
(6) sterilize, be dry: the lysate of step (5) is sterilized, after lysate is freeze-dried；
(7) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist.
The soyabean protein powder obtained to implementation 6 detects, as a result as follows:
Embodiment 7: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) extract: using soybean low temperature soy meal as raw material, be put into extractor by 1(soybean low temperature soy meal): 8(water) ratio
Water is added in example, and water temperature control is 30 DEG C, and sodium hydroxide solution (pH=10.5) are added and adjust pH value to 9.0, control stirring rate
500 turns/min, 120min is stirred, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) acid is heavy: it is added hydrochloric acid (pH=5), the PH to 5.5 of the water containing albumen in the heavy tank of adjustment acid, 8 turns of stirring rate of control/
Min is stirred 3 hours, is continuously added hydrochloric acid (pH=5) and is adjusted pH to 4.6, control 8 turns/min of stirring rate, stirs 2 hours；
(4) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(5) neutralize: sodium hydroxide solution (pH=10.5) are added in the curd-containing canisters 1 in step (4), adjust PH and are adjusted to 7.5；
(6) sterilize, be dry: the lysate of step (5) is sterilized, after lysate is freeze-dried；
(7) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist.
The soyabean protein powder obtained to implementation 7 detects, as a result as follows:
Embodiment 8: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) extract: using soybean low temperature soy meal as raw material, being put into extractor by 1(soybean low temperature soy meal): 8.2(water)
Water is added in ratio, and water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, control stirring rate 700
Turn/min, stirs 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) acid is heavy: it is added hydrochloric acid (pH=4), the PH to 5.2 of the water containing albumen in the heavy tank of adjustment acid, 7 turns of stirring rate of control/
Min is stirred 2 hours, is continuously added hydrochloric acid (pH=4) and is adjusted pH to 4.5, control 7 turns/min of stirring rate, stirs 3 hours；
(4) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(5) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 1 in step (4), adjust PH and are adjusted to 7.0；
(6) sterilize, be dry: the lysate of step (5) is sterilized, after lysate is freeze-dried；
(7) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist.
The soyabean protein powder obtained to implementation 8 detects, as a result as follows:
Comparative analysis embodiment 1-3 and embodiment 6-8 can be reduced significantly in soyabean protein powder using the heavy method of two acid
The content of ash content, is obviously improved coloration.
Embodiment 9: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) it extracts: using soybean low temperature soy meal as raw material, 0.5Kg dimeticone is added, is put into extractor low by 1(soybean
Warm dregs of beans): 8.2(water) ratio be added water, water temperature control be 32 DEG C, be added sodium carbonate liquor (pH=10) adjust pH value to
8.8,700 turns/min of stirring rate is controlled, stirs 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) using the isolated bean dregs of step (2) as raw material, be put into extractor in 1(bean dregs): 5(water) ratio add
Enter water, water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, stir, the protein dissolution in bean dregs
Yu Shuizhong；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: it is added hydrochloric acid (pH=4), the PH to 5.2 of the water containing albumen in the heavy tank of adjustment acid, 7 turns of stirring rate of control/
Min is stirred 2 hours, is continuously added hydrochloric acid (pH=4) and is adjusted pH to 4.5, control 7 turns/min of stirring rate, stirs 3 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) washing: by 1(curdled milk): 4(weight ratio) add water to enter in curd-containing canisters, it stirs, separation, protein liquid (referred to as: curdled milk), which arrives, to coagulate
Newborn tank 2；
(7) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 2 in step (6), adjust PH and are adjusted to 7.0；
(8) sterilize, be dry: the lysate of step (7) is sterilized, after lysate is freeze-dried；
(9) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist.
The soyabean protein powder obtained to implementation 9 detects, as a result as follows:
Comparative analysis embodiment 5 and embodiment 9 are added dimeticone, can be obviously improved the yield of soyabean protein powder.
Embodiment 10: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) it extracts: using soybean low temperature soy meal as raw material, 0.5Kg sodium pyrosulfite is added, is put into extractor by 1(soybean
Low temperature soy meal): 8.2(water) ratio be added water, water temperature control be 32 DEG C, be added sodium carbonate liquor (pH=10) adjust pH value to
8.8,700 turns/min of stirring rate is controlled, stirs 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) using the isolated bean dregs of step (2) as raw material, be put into extractor in 1(bean dregs): 5(water) ratio add
Enter water, water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, stir, the protein dissolution in bean dregs
Yu Shuizhong；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: it is added hydrochloric acid (pH=4), the PH to 5.2 of the water containing albumen in the heavy tank of adjustment acid, 7 turns of stirring rate of control/
Min is stirred 2 hours, is continuously added hydrochloric acid (pH=4) and is adjusted pH to 4.5, control 7 turns/min of stirring rate, stirs 3 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) washing: by 1(curdled milk): 4(weight ratio) add water to enter in curd-containing canisters, it stirs, separation, protein liquid (referred to as: curdled milk), which arrives, to coagulate
Newborn tank 2；
(7) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 2 in step (6), adjust PH and are adjusted to 7.0；
(8) sterilize, be dry: the lysate of step (7) is sterilized, after lysate is freeze-dried；
(9) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist.
The soyabean protein powder obtained to implementation 10 detects, as a result as follows:
Comparative analysis embodiment 5 and embodiment 10 are added sodium pyrosulfite, can be obviously improved the coloration of soyabean protein powder.
Embodiment 11: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) it extracts: using soybean low temperature soy meal as raw material, 0.5Kg dimeticone, 0.5Kg sodium pyrosulfite is added, is put into extraction
Take in tank in 1(soybean low temperature soy meal): 8.2(water) ratio be added water, water temperature control be 32 DEG C, be added sodium carbonate liquor (pH=
10) pH value is adjusted to 8.8, is controlled 700 turns/min of stirring rate, is stirred 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) using the isolated bean dregs of step (2) as raw material, be put into extractor in 1(bean dregs): 5(water) ratio add
Enter water, water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, stir, the protein dissolution in bean dregs
Yu Shuizhong；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: it is added hydrochloric acid (pH=4), the PH to 5.2 of the water containing albumen in the heavy tank of adjustment acid, 7 turns of stirring rate of control/
Min is stirred 2 hours, is continuously added hydrochloric acid (pH=4) and is adjusted pH to 4.5, control 7 turns/min of stirring rate, stirs 3 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) washing: by 1(curdled milk): 4(weight ratio) add water to enter in curd-containing canisters, it stirs, separation, protein liquid (referred to as: curdled milk), which arrives, to coagulate
Newborn tank 2；
(7) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 2 in step (6), adjust PH and are adjusted to 7.0；
(8) sterilize, be dry: the lysate of step (7) is sterilized, after lysate is freeze-dried；
(9) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist.
The soyabean protein powder obtained to implementation 11 detects, as a result as follows:
Embodiment 12: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) it extracts: using soybean low temperature soy meal as raw material, 0.5g, dimeticone, 0.5Kg sodium pyrosulfite is added, is put into extraction
Take in tank in 1(soybean low temperature soy meal): 8.2(water) ratio be added water, water temperature control be 32 DEG C, be added sodium carbonate liquor (pH=
10) pH value is adjusted to 8.8, is controlled 700 turns/min of stirring rate, is stirred 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) using the isolated bean dregs of step (2) as raw material, be put into extractor in 1(bean dregs): 5(water) ratio add
Enter water, water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, stir, the protein dissolution in bean dregs
Yu Shuizhong；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: it is added hydrochloric acid (pH=4), the PH to 5.2 of the water containing albumen in the heavy tank of adjustment acid, 7 turns of stirring rate of control/
Min is stirred 2 hours, is continuously added hydrochloric acid (pH=4) and is adjusted pH to 4.5, control 7 turns/min of stirring rate, stirs 3 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) washing: by 1(curdled milk): 4(weight ratio) add water to enter in curd-containing canisters, it stirs, separation, protein liquid (referred to as: curdled milk), which arrives, to coagulate
Newborn tank 2；
(7) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 2 in step (6), adjust PH and are adjusted to 7.0；
(8) sterilize, be dry: the lysate of step (7) is sterilized, after lysate is freeze-dried；
(9) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 40 microns of micro mist.
The soyabean protein powder obtained to implementation 12 detects, as a result as follows:
Embodiment 13: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) it extracts: using soybean low temperature soy meal as raw material, 0.5g, dimeticone, 0.5Kg sodium pyrosulfite is added, is put into extraction
Take in tank in 1(soybean low temperature soy meal): 8.2(water) ratio be added water, water temperature control be 32 DEG C, be added sodium carbonate liquor (pH=
10) pH value is adjusted to 8.8, is controlled 700 turns/min of stirring rate, is stirred 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) using the isolated bean dregs of step (2) as raw material, be put into extractor in 1(bean dregs): 5(water) ratio add
Enter water, water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, stir, the protein dissolution in bean dregs
Yu Shuizhong；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: it is added hydrochloric acid (pH=4), the PH to 5.2 of the water containing albumen in the heavy tank of adjustment acid, 7 turns of stirring rate of control/
Min is stirred 2 hours, is continuously added hydrochloric acid (pH=4) and is adjusted pH to 4.5, control 7 turns/min of stirring rate, stirs 3 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) washing: by 1(curdled milk): 4(weight ratio) add water to enter in curd-containing canisters, it stirs, separation, protein liquid (referred to as: curdled milk), which arrives, to coagulate
Newborn tank 2；
(7) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 2 in step (6), adjust PH and are adjusted to 7.0；
(8) sterilize, be dry: the lysate of step (7) is sterilized, after lysate is freeze-dried；
(9) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 32 microns of micro mist.
The soyabean protein powder obtained to implementation 13 detects, as a result as follows:
Embodiment 14: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) it extracts: using soybean low temperature soy meal as raw material, 0.5g, dimeticone, 0.5Kg sodium pyrosulfite is added, is put into extraction
Take in tank in 1(soybean low temperature soy meal): 8.2(water) ratio be added water, water temperature control be 32 DEG C, be added sodium carbonate liquor (pH=
10) pH value is adjusted to 8.8, is controlled 700 turns/min of stirring rate, is stirred 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) using the isolated bean dregs of step (2) as raw material, be put into extractor in 1(bean dregs): 5(water) ratio add
Enter water, water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, stir, the protein dissolution in bean dregs
Yu Shuizhong；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: it is added hydrochloric acid (pH=4), the PH to 5.2 of the water containing albumen in the heavy tank of adjustment acid, 7 turns of stirring rate of control/
Min is stirred 2 hours, is continuously added hydrochloric acid (pH=4) and is adjusted pH to 4.5, control 7 turns/min of stirring rate, stirs 3 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) washing: by 1(curdled milk): 4(weight ratio) add water to enter in curd-containing canisters, it stirs, separation, protein liquid (referred to as: curdled milk), which arrives, to coagulate
Newborn tank 2；
(7) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 2 in step (6), adjust PH and are adjusted to 7.0；
(8) sterilize, be dry: the lysate of step (7) is sterilized, after lysate is freeze-dried；
(9) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 25 microns of micro mist.
The soyabean protein powder obtained to implementation 14 detects, as a result as follows:
Comparative analysis embodiment 5, embodiment 12-14, micronization step significantly affect the solubility property of soyabean protein powder, work as partial size
For range in the range of 32 ~ 50 microns, partial size is smaller, and the dissolubility of albumen powder is obviously improved, when particle size range is lower than 32 microns
When, influence of the partial size to the solubility property of albumen powder is smaller.
Embodiment 15: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) it extracts: using soybean low temperature soy meal as raw material, 0.5g, dimeticone, 0.5Kg sodium pyrosulfite is added, is put into extraction
Take in tank in 1(soybean low temperature soy meal): 8.2(water) ratio be added water, water temperature control be 32 DEG C, be added sodium carbonate liquor (pH=
10) pH value is adjusted to 8.8, is controlled 700 turns/min of stirring rate, is stirred 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) using the isolated bean dregs of step (2) as raw material, be put into extractor in 1(bean dregs): 5(water) ratio add
Enter water, water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, stir, the protein dissolution in bean dregs
Yu Shuizhong；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: being added citric acid (pH=4), adjusts the PH to 5.2 of the water containing albumen in the heavy tank of acid, control stirring rate 7
Turn/min, stir 2 hours, continuously adds hydrochloric acid (pH=4) and adjust pH to 4.5, control 7 turns/min of stirring rate, stir 3 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) washing: by 1(curdled milk): 4(weight ratio) add water to enter in curd-containing canisters, it stirs, separation, protein liquid (referred to as: curdled milk), which arrives, to coagulate
Newborn tank 2；
(7) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 2 in step (6), adjust PH and are adjusted to 7.0；
(8) sterilize, be dry: the lysate of step (7) is sterilized, after lysate is freeze-dried；
(9) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 320 microns of micro mist.
The soyabean protein powder obtained to implementation 15 detects, as a result as follows:
Comparative example 1: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) extract: using soybean low temperature soy meal as raw material, being put into extractor by 1(soybean low temperature soy meal): 8.2(water)
Water is added in ratio, and water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, control stirring rate 700
Turn/min, stirs 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) acid is heavy: it is added hydrochloric acid (pH=4), adjusts the PH to 5.2 of the water containing albumen in the heavy tank of acid, stand growing the grain 2 hours, after
Continuous be added hydrochloric acid (pH=4) adjust pH to 4.5, stand growing the grain 3 hours.
(4) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, the protein body of precipitating is separated from water,
Protein liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(5) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 1 in step (4), adjust PH and are adjusted to 7.0；
(6) sterilize, be dry: the lysate of step (5) is sterilized, after lysate is freeze-dried；
(7) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist.
The soyabean protein powder obtained to comparative example 1 detects, as a result as follows:
Comparative example 2: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) extract: using soybean low temperature soy meal as raw material, being put into extractor by 1(soybean low temperature soy meal): 8.2(water)
Water is added in ratio, and water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, control stirring rate 700
Turn/min, stirs 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) acid is heavy: being added hydrochloric acid (pH=4), adjusts the PH to 5.2 of the water containing albumen in the heavy tank of acid, control stirring rate 10
Turn/min, stir 2 hours, continuously add hydrochloric acid (pH=4) and adjust pH to 4.5, control 10 turns/min of stirring rate, stirring 3 is small
When；
(4) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(5) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 1 in step (4), adjust PH and are adjusted to 7.0；
(6) sterilize, be dry: the lysate of step (5) is sterilized, after lysate is freeze-dried；
(7) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist.
The soyabean protein powder obtained to comparative example 2 detects, as a result as follows:
Comparative example 3: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) extract: using soybean low temperature soy meal as raw material, being put into extractor by 1(soybean low temperature soy meal): 8.2(water)
Water is added in ratio, and water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, control stirring rate 700
Turn/min, stirs 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) acid is heavy: it is added hydrochloric acid (pH=4), the PH to 5.2 of the water containing albumen in the heavy tank of adjustment acid, 7 turns of stirring rate of control/
Min is stirred 0.5 hour, is continuously added hydrochloric acid (pH=4) and is adjusted pH to 4.5, control 10 turns/min of stirring rate, stirs 1 hour；
(4) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(5) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 1 in step (4), adjust PH and are adjusted to 7.0；
(6) sterilize, be dry: the lysate of step (5) is sterilized, after lysate is freeze-dried；
(7) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist.
The soyabean protein powder obtained to comparative example 3 detects, as a result as follows:
Comparative analysis embodiment 6-8 and comparative example 1-3, two acid sink stirring rate and rearing crystal time in method etc., significant shadow
Ring ash content, coloration and the content of finished product soyabean protein powder.
Best two acid sinks method are as follows: the PH to 5.1 ~ 5.5 of the water containing albumen in the heavy tank of acid adjustment acid is added, is slowly stirred
1 ~ 3 hour, acid for adjusting pH was continuously added to 4.4 ~ 4.6, is slowly stirred 2 ~ 4 hours.Described is slowly stirred as 6 ~ 8 turns/min.
Comparative example 4: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) it extracts: using soybean low temperature soy meal as raw material, 0.2Kg sodium pyrosulfite is added, is put into extractor by 1(soybean
Low temperature soy meal): 8.2(water) ratio be added water, water temperature control be 32 DEG C, be added sodium carbonate liquor (pH=10) adjust pH value to
8.8,700 turns/min of stirring rate is controlled, stirs 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) using the isolated bean dregs of step (2) as raw material, be put into extractor in 1(bean dregs): 5(water) ratio add
Enter water, water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, stir, the protein dissolution in bean dregs
Yu Shuizhong；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: it is added hydrochloric acid (pH=4), the PH to 5.2 of the water containing albumen in the heavy tank of adjustment acid, 7 turns of stirring rate of control/
Min is stirred 2 hours, is continuously added hydrochloric acid (pH=4) and is adjusted pH to 4.5, control 7 turns/min of stirring rate, stirs 3 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) washing: by 1(curdled milk): 4(weight ratio) add water to enter in curd-containing canisters, it stirs, separation, protein liquid (referred to as: curdled milk), which arrives, to coagulate
Newborn tank 2；
(7) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 2 in step (6), adjust PH and are adjusted to 7.0；
(8) sterilize, be dry: the lysate of step (7) is sterilized, after lysate is freeze-dried；
(9) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 98%
Above by 150 meshes.
The soyabean protein powder obtained to comparative example 4 detects, as a result as follows:
Comparative example 5: the preparation of instant soluble soybean albumen powder
Soybean low temperature soy meal: 50Kg(CP:58%, NSI(nitrogen soluble index): 88%)
(1) it extracts: using soybean low temperature soy meal as raw material, 1.0Kg sodium pyrosulfite is added, is put into extractor by 1(soybean
Low temperature soy meal): 8.2(water) ratio be added water, water temperature control be 32 DEG C, be added sodium carbonate liquor (pH=10) adjust pH value to
8.8,700 turns/min of stirring rate is controlled, stirs 90min, the protein dissolution in dregs of beans is in water；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) using the isolated bean dregs of step (2) as raw material, be put into extractor in 1(bean dregs): 5(water) ratio add
Enter water, water temperature control is 32 DEG C, and sodium carbonate liquor (pH=10) are added and adjust pH value to 8.8, stir, the protein dissolution in bean dregs
Yu Shuizhong；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: it is added hydrochloric acid (pH=4), the PH to 5.2 of the water containing albumen in the heavy tank of adjustment acid, 7 turns of stirring rate of control/
Min is stirred 2 hours, is continuously added hydrochloric acid (pH=4) and is adjusted pH to 4.5, control 7 turns/min of stirring rate, stirs 3 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) washing: by 1(curdled milk): 4(weight ratio) add water to enter in curd-containing canisters, it stirs, separation, protein liquid (referred to as: curdled milk), which arrives, to coagulate
Newborn tank 2；
(7) neutralize: sodium carbonate liquor (pH=10) are added in the curd-containing canisters 2 in step (6), adjust PH and are adjusted to 7.0；
(8) sterilize, be dry: the lysate of step (7) is sterilized, after lysate is freeze-dried；
(9) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 98%
Above by 200 meshes.
The soyabean protein powder obtained to comparative example 5 detects, as a result as follows:
Micronization step significantly affects the solubility property of soyabean protein powder, and micronization step significantly affects the dissolution of soyabean protein powder
Performance, when particle size range becomes larger, such as when by 150 mesh or 200 mesh, the solubility property of soyabean protein powder is deteriorated, point
The scattered time is long, and layering is fast, and instant and stable effect is not achieved.
Claims (6)
1. a kind of instant soluble soybean albumen powder, which is characterized in that jitter time was made by the steps less than 15 seconds:
(1) extract: using soybean low temperature soy meal as raw material, being put into extractor by 1(soybean low temperature soy meal): 8 ~ 9(water)
Water is added in ratio, and water temperature control is 30 ~ 35 DEG C, addition aqueous slkali adjusting pH value to 8.5 ~ 9.0, stirring, and the albumen in dregs of beans is molten
In Xie Yushui；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) optionally, using the isolated bean dregs of step (2) as raw material, it is put into extractor by 1(bean dregs): 4 ~ 5(water)
Ratio water is added, water temperature control is 30 ~ 35 DEG C, aqueous slkali is added and adjusts pH value to 8.5 ~ 9.0, stirring, the albumen in bean dregs
It is dissolved in the water；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: acid is added, adjusts the pH to 4.4 ~ 4.6 of the water containing albumen in the heavy tank of acid；Stirring 3 ~ 5 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) optionally, washing: by 1(curdled milk): 3 ~ 5(weight ratio) add water to enter in curd-containing canisters, it stirs, separation, protein liquid (abbreviation:
Curdled milk) arrive curd-containing canisters 2；
(7) it neutralizes: aqueous slkali is added in the curd-containing canisters 1 in step (4) or the curd-containing canisters 2 in step (5), adjusts PH and is adjusted to
6.5~7.5；
(8) sterilize, be dry: the lysate of step (6) is sterilized, after lysate is dry；
(9) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist；
In the step (1), the aqueous slkali that the preferred pH of the aqueous slkali is 9.5 ~ 10.5, more preferable pH is 9.5 ~ 10.5
Sodium carbonate liquor, sodium hydroxide solution, potassium hydroxide solution；
In the step (1), soybean low temperature soy meal, CP(butt) it is not less than 55%, NSI(nitrogen soluble index) it is not less than 85%；
In the step (1), stirring rate is 500 ~ 1000 turns/min(minutes), mixing time is 60 ~ 120min；
The step (7), the aqueous slkali that the preferred pH of the aqueous slkali is 9.5 ~ 10.5, the carbon that more preferable pH is 9.5 ~ 10.5
Acid sodium solution, sodium hydroxide solution, potassium hydroxide solution；
The step (8), the drying can be high and dry for constant-pressure and high-temperature, or decompression is high and dry, or freezing
It is dry, preferably freeze drying.
2. a kind of preparation method of instant soluble soybean albumen powder as described in claim 1, feature are being, including are walking as follows
It is rapid:
(1) extract: using soybean low temperature soy meal as raw material, being put into extractor by 1(soybean low temperature soy meal): 8 ~ 9(water)
Water is added in ratio, and water temperature control is 30 ~ 35 DEG C, addition aqueous slkali adjusting pH value to 8.5 ~ 9.0, stirring, and the albumen in dregs of beans is molten
In Xie Yushui；
(2) it separates: low temperature soy meal solution being sent into high speed separator, the bean dregs in mixed solution are separated with the water containing albumen
It opens, in the heavy tank of water merging acid containing albumen；
(3) optionally, using the isolated bean dregs of step (2) as raw material, it is put into extractor by 1(bean dregs): 4 ~ 5(water)
Ratio water is added, water temperature control is 30 ~ 35 DEG C, aqueous slkali is added and adjusts pH value to 8.5 ~ 9.0, stirring, the albumen in bean dregs
It is dissolved in the water；Separation, the heavy tank of water merging acid containing albumen；
(4) acid is heavy: acid is added, adjusts the pH to 4.4 ~ 4.6 of the water containing albumen in the heavy tank of acid；Stirring 3 ~ 5 hours；
(5) it separates: mixed liquor of the acid after heavy being sent into seperator and is separated, is separated from water the protein body of precipitating, albumen
Liquid (referred to as: curdled milk) arrives curd-containing canisters 1；
(6) optionally, washing: by 1(curdled milk): 3 ~ 5(weight ratio) add water to enter in curd-containing canisters, it stirs, separation, protein liquid (abbreviation:
Curdled milk) arrive curd-containing canisters 2；
(7) it neutralizes: aqueous slkali is added in the curd-containing canisters 1 in step (4) or the curd-containing canisters 2 in step (5), adjusts PH and is adjusted to
6.5~7.5；
(8) sterilize, be dry: the lysate of step (6) is sterilized, after lysate is dry；
(9) it is micronized: dry soybean protein isolate being smashed, product is crushed with micronizer, obtains 90%
The above partial size is no more than 50 microns of micro mist；
In the step (1), the aqueous slkali that the preferred pH of the aqueous slkali is 9.5 ~ 10.5, more preferable pH is 9.5 ~ 10.5
Sodium carbonate liquor, sodium hydroxide solution, potassium hydroxide solution；
In the step (1), soybean low temperature soy meal, CP(butt) it is not less than 55%, NSI(nitrogen soluble index) it is not less than 85%；
In the step (1), stirring rate is 500 ~ 1000 turns/min(minutes), mixing time is 60 ~ 120min；
The step (7), the aqueous slkali that the preferred pH of the aqueous slkali is 9.5 ~ 10.5, the carbon that more preferable pH is 9.5 ~ 10.5
Acid sodium solution, sodium hydroxide solution, potassium hydroxide solution；
The step (8), the drying can be high and dry for constant-pressure and high-temperature, or decompression is high and dry, or freezing
It is dry, preferably freeze drying.
3. preparation method as claimed in claim 2, which is characterized in that the step (4), can two step acid sink method, specifically
Are as follows: (4) acid is heavy: the PH to 5.1 ~ 5.5 of the water containing albumen in the heavy tank of addition acid adjustment acid is slowly stirred 1 ~ 3 hour, continues to add
Enter acid for adjusting pH to 4.4 ~ 4.6, be slowly stirred 2 ~ 4 hours, described is slowly stirred as 6 ~ 8 turns/min.
4. preparation method as claimed in claim 2, which is characterized in that in the step (1), low temperature soy meal can also be added
The defoaming agent of weight 0.5% ~ 1%, the defoaming agent can be with dimeticone or Simethicones, preferably Simethicone.
5. preparation method as claimed in claim 2, which is characterized in that in the step (1), low temperature soy meal can also be added
The stabilizer of weight 0.5% ~ 1%, the anti-stabilizer are sodium pyrosulfite.
6. preparation method as claimed in claim 2, which is characterized in that the step (8), it is micro- that the partial size is no more than 50
The micro mist of rice, preferable particle size are no more than 400 microns of micro mist, more preferably no more than 320 microns of micro mist.
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| CN110122654A (en) * | 2019-06-19 | 2019-08-16 | 山东御馨生物科技有限公司 | A kind of cleaning processing method of soybean protein |
| CN111493151A (en) * | 2020-05-13 | 2020-08-07 | 杨德威 | Milk powder for promoting growth and development of children skeleton and preparation method thereof |
| CN111567810A (en) * | 2020-05-22 | 2020-08-25 | 天益食品（徐州）有限公司 | Burdock root protein composite health preserving powder and preparation method thereof |
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| CN113647505A (en) * | 2021-08-26 | 2021-11-16 | 汤臣倍健股份有限公司 | Instant soybean protein powder and preparation method thereof |
| CN114468115A (en) * | 2022-02-22 | 2022-05-13 | 克东禹王大豆蛋白食品有限公司 | Production method of low-hydration-viscosity bitter-free high-whiteness soybean protein isolate |
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| CN110122654A (en) * | 2019-06-19 | 2019-08-16 | 山东御馨生物科技有限公司 | A kind of cleaning processing method of soybean protein |
| CN111493151A (en) * | 2020-05-13 | 2020-08-07 | 杨德威 | Milk powder for promoting growth and development of children skeleton and preparation method thereof |
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| CN114480546A (en) * | 2022-03-01 | 2022-05-13 | 佳木斯冬梅大豆食品有限公司 | Method for improving yield of low-molecular-weight soybean peptide |
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