CN108014154A - A kind of method for extracting arasaponin - Google Patents

A kind of method for extracting arasaponin Download PDF

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Publication number
CN108014154A
CN108014154A CN201711269145.1A CN201711269145A CN108014154A CN 108014154 A CN108014154 A CN 108014154A CN 201711269145 A CN201711269145 A CN 201711269145A CN 108014154 A CN108014154 A CN 108014154A
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ginseng
fresh
pseudo
raw material
arasaponin
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韩业详
王洪
侯嘉
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SICHUAN JINSUIFANG PHARMACEUTICAL CO Ltd
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SICHUAN JINSUIFANG PHARMACEUTICAL CO Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a kind of method for extracting arasaponin, include the following steps：Select maturity good, bright in colour, full grains are complete, and no disease and pests harm, go mouldy is used as raw material with the fresh pseudo-ginseng material of mechanical damage, is then cleaned, cleaning, and shady place is dried is broken into the fresh particle of 20 40 purposes after the moisture content of surface with pulverizer；The fresh particulate samples of 20 40 mesh of fresh pseudo-ginseng crushed are put in the molten device of extraction, the present invention is using fresh pseudo-ginseng as raw material, recovery rate is not less than corresponding dry pseudo-ginseng raw material, comprehensive cost is less than extracts notoginsenoside by raw material of dry pseudo-ginseng, cycle is short, cost is low, suitable for industrialized production, at the same time, exploitation extracts the deep process technology of saponin(e using fresh pseudo-ginseng as raw material, the present invention is with using microwave radiation technology enzymolysis extraction arasaponin, compared with other extracting modes, substantially reduce the test period, at the same time, microwave is at least saponin(e leaching rate in pseudo-ginseng 4 times without microwave treatment.

Description

A kind of method for extracting arasaponin
Technical field
The present invention relates to technical field of natural product extraction, is specially a kind of method for extracting arasaponin.
Background technology
As scientific technological advance is advanced by leaps and bounds, various new technologies, new invention emerge in an endless stream, and are applied rapidly and serviced In society, expanding economy is greatly facilitated, pseudo-ginseng is araliaceae ginseng plant, and main product is in Yunnan, first recorded in Compendium of Materia Medica, For China's tradition rare traditional Chinese medicine, there is promoting blood circulation and removing blood stasis, detumescence ding-tong, hemostasia and dissipation blood stasis and other effects, pseudo-ginseng different parts contain There is more than 20 kinds of saponin constituent, its main active is arasaponin, and arasaponin has increase coronary blood flow, protects Brain tissue is protected, expands the effect such as blood vessel and decompression.
Current extraction process for notoginseng saponin mainly has decoction alcohol precipitation method, ethanol refluxing process and percolation.Wherein decocting Alcohol deposition method is easily gelatinized during water decoction because pseudo-ginseng contains substantial amounts of starch and polysaccharide；Due to the suction of precipitation after alcohol precipitation Attached effect saponin(e loss is larger, and recovery rate is relatively low, and the content less stable of product component, is chiefly used in the exploitation of food；Diacolation Method recovery rate is higher, easy to operate, and extracting solution impurities are few, but solvent consumption is excessive, are mainly used for medicine；Alcohol reflux Method method is easy, and process conditions are easily controllable, but recovery rate is relatively low, and these types of extracting method is required to raw material and selects drying Rhizomes of Panax notoginseng (clip), processing leftover pieces extracted, hence it is evident that extend extracting cycle.
And emerging enzymatic isolation method and the above method such as decoction alcohol precipitation method are combined, so as to extract the total soap of pseudo-ginseng in fresh pseudo-ginseng Glycosides, effectively shortens the process-cycle, increases the yield of notoginsenoside, improves extraction efficiency, but in traditional enzymatic isolation method Enzymolysis processing is carried out using cellulase or pectase more, mixed enzymolysis processing is seldom but carried out using microwave radiation technology.
Meanwhile using dry pseudo-ginseng to extract notoginsenoside for raw material now more, cycle length is of high cost, is unfavorable for related industry It is intensive, be also unfavorable for market regulation, the control ability of Yunnan notoginseng, skill upgrading to enterprise of one's native land, economic benefit increase It is long, have a negative impact to the sustainable development for promoting pseudo-ginseng industry.
The content of the invention
It is an object of the invention to provide a kind of method for extracting arasaponin, and extraction pseudo-ginseng is digested using microwave radiation technology Total saposins have the advantages that efficient, energy- and time-economizing, pollution are small, to solve the problems mentioned in the above background technology.
To achieve the above object, the present invention provides following technical solution：
A kind of method for extracting arasaponin, includes the following steps：
S1, selects maturity good, and bright in colour, full grains are complete, no disease and pests harm, go mouldy with mechanical damage fresh three Then seven materials are cleaned as raw material, cleaned, and shady place is dried is broken into 20-40 purposes after the moisture content of surface with pulverizer Fresh particle；
S2, the fresh fresh particulate samples of pseudo-ginseng 20-40 mesh crushed are put in the molten device of extraction, are added complex enzyme and are carried out at enzymolysis 4-6h is managed, PH 4.5, microwave radiation technology enzymolysis temperature is 50-70 DEG C, and microwave power 500-580W, complex enzyme is by cellulase 10-20U/g (crude drug), pectase 120-160U/g (crude drug) are formed, and are added the 6-10 times of 50%-90% measured ethanol and are carried out Refluxing extraction, filtering, collects filtrate, spare；
Ethanol is recovered under reduced pressure in S3, concentration, vacuum 0.075-0.08 pas, and 60-70 DEG C of temperature, obtains fresh pseudo-ginseng extracting solution；
S4, reflux decoloration 10-3Omin, filters, cools down, fresh pseudo-ginseng extracting solution is by being covered with a small amount of neutral alumina while hot Funnel, ethanol eluate is recovered under reduced pressure, it is spare；
S5, for fresh pseudo-ginseng extracting solution by the large pore resin absorption column of pretreatment, resin demand is 1.2-1.8 times of raw material, First with distillation water elution, it is colourless to be washed till efflux, is decolourized by cation exchange resin column, uses 60%-80% ethanol instead Xian takes off liquid, and elution terminal is checked with TLC, and ethanol eluate adds 1% activated carbon (by the gauge that feeds intake), collects efflux, concentration To doing, up to arasaponin.
Preferably, the rotating speed of the pulverizer is 100-300r/min, and the bottom discharge mouth bottom activity of pulverizer is even It is connected to fine mesh screen.
Preferably, strong acidic ion resin or acidulous cation resin can be selected in the cation exchange resin.
Preferably, the inspissator in the S3 includes inspissator, condenser, vapor-liquid separation using vacuum-concentrcted device Device, cooler, by five component of liquid bucket, inspissator is jacket structured, and condenser is shell and tube, and cooler is coiled structure, if It is standby to use stainless steel making with material contact portion, there is good corrosion resistance.
Preferably, in the S4 funnel top cross-sectional diameter be more than its bottom cross-sectional diameter.
Compared with prior art, the beneficial effects of the invention are as follows：
1st, for the present invention using fresh pseudo-ginseng as raw material, recovery rate is not less than corresponding dry pseudo-ginseng raw material, and comprehensive cost is less than with dry Pseudo-ginseng for raw material extract notoginsenoside, the cycle is short, and cost is low, suitable for industrialized production, meanwhile, exploitation using fresh pseudo-ginseng as Raw material extracts the deep process technology of saponin(e, and local enterprise can be made to give full play to using place of production advantage, be directly involved pseudo-ginseng industrial chain Middle reaches, improve added value of product；
2nd, the present invention compared with other extracting modes, is greatly shortened with extracting arasaponin using microwave radiation technology enzymolysis Test period, meanwhile, microwave is at least saponin(e leaching rate in pseudo-ginseng 4 times without microwave treatment.
Embodiment
Below in conjunction with the embodiment of the present invention, the technical solution in the embodiment of the present invention is clearly and completely described, Obviously, described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.Based in the present invention Embodiment, those of ordinary skill in the art's all other embodiments obtained without making creative work, all Belong to the scope of protection of the invention.
Embodiment 1
A kind of method for extracting arasaponin, includes the following steps：
S1, selects maturity good, and bright in colour, full grains are complete, no disease and pests harm, go mouldy with mechanical damage fresh three Then seven materials are cleaned as raw material, cleaned, and shady place is dried is broken into fresh of 20 purposes after the moisture content of surface with pulverizer Grain；
S2, the fresh particulate samples of 20 mesh of fresh pseudo-ginseng crushed are put in the molten device of extraction, are added complex enzyme and are carried out enzymolysis processing 4h, PH 4.5, microwave radiation technology enzymolysis temperature is 50 DEG C, microwave power 500W, and complex enzyme is by cellulase 10U/g (crude drug), fruit Glue enzyme 120U/g (crude drug) is formed, and 50% ethanol for adding 6 times of amounts carries out refluxing extraction, and filtering, collects filtrate, spare；
Ethanol is recovered under reduced pressure in S3, concentration, 0.075 pa of vacuum, and temperature 60 C, obtains fresh pseudo-ginseng extracting solution；
S4, reflux decoloration 10min, filters, cools down, fresh pseudo-ginseng extracting solution is by being covered with the leakage of a small amount of neutral alumina while hot Bucket, is recovered under reduced pressure ethanol eluate, spare；
S5, for fresh pseudo-ginseng extracting solution by the large pore resin absorption column of pretreatment, resin demand is 1.2 times of raw material, is first used Water elution is distilled, it is colourless to be washed till efflux, decolourizes by cation exchange resin column, uses 60% ethanol Xian instead and take off liquid, uses TLC checks elution terminal, and ethanol eluate adds 1% activated carbon (by the gauge that feeds intake), collects efflux, is concentrated to dryness, to obtain the final product Arasaponin.
Specifically, the rotating speed of the pulverizer is 100r/min, and the bottom discharge mouth bottom of pulverizer is connected with Fine mesh screen.
Specifically, strong acidic ion resin or acidulous cation resin can be selected in the cation exchange resin.
Specifically, the inspissator in the S3 includes inspissator, condenser, vapor-liquid separation using vacuum-concentrcted device Device, cooler, by five component of liquid bucket, inspissator is jacket structured, and condenser is shell and tube, and cooler is coiled structure, if It is standby to use stainless steel making with material contact portion, there is good corrosion resistance.
Specifically, the top cross-sectional diameter of funnel is more than the cross-sectional diameter of its bottom in the S4.
Embodiment 2
A kind of method for extracting arasaponin, includes the following steps：
S1, selects maturity good, and bright in colour, full grains are complete, no disease and pests harm, go mouldy with mechanical damage fresh three Then seven materials are cleaned as raw material, cleaned, and shady place is dried is broken into fresh of 30 purposes after the moisture content of surface with pulverizer Grain；
S2, the fresh particulate samples of 30 mesh of fresh pseudo-ginseng crushed are put in the molten device of extraction, are added complex enzyme and are carried out enzymolysis processing 5h, PH 4.5, microwave radiation technology enzymolysis temperature is 60 DEG C, microwave power 540W, and complex enzyme is by cellulase 15U/g (crude drug), fruit Glue enzyme 140U/g (crude drug) is formed, and 70% ethanol for adding 8 times of amounts carries out refluxing extraction, and filtering, collects filtrate, spare；
Ethanol is recovered under reduced pressure in S3, concentration, 0.078 pa of vacuum, and 65 DEG C of temperature, obtains fresh pseudo-ginseng extracting solution；
S4, reflux decoloration 2Omin, filters, cools down, fresh pseudo-ginseng extracting solution is by being covered with the leakage of a small amount of neutral alumina while hot Bucket, is recovered under reduced pressure ethanol eluate, spare；
S5, for fresh pseudo-ginseng extracting solution by the large pore resin absorption column of pretreatment, resin demand is 1.6 times of raw material, is first used Water elution is distilled, it is colourless to be washed till efflux, decolourizes by cation exchange resin column, uses 70% ethanol Xian instead and take off liquid, uses TLC checks elution terminal, and ethanol eluate adds 1% activated carbon (by the gauge that feeds intake), collects efflux, is concentrated to dryness, to obtain the final product Arasaponin.
Specifically, the rotating speed of the pulverizer is 200r/min, and the bottom discharge mouth bottom of pulverizer is connected with Fine mesh screen.
Specifically, strong acidic ion resin or acidulous cation resin can be selected in the cation exchange resin.
Specifically, the inspissator in the S3 includes inspissator, condenser, vapor-liquid separation using vacuum-concentrcted device Device, cooler, by five component of liquid bucket, inspissator is jacket structured, and condenser is shell and tube, and cooler is coiled structure, if It is standby to use stainless steel making with material contact portion, there is good corrosion resistance.
Specifically, the top cross-sectional diameter of funnel is more than the cross-sectional diameter of its bottom in the S4.
Embodiment 3
A kind of method for extracting arasaponin, includes the following steps：
S1, selects maturity good, and bright in colour, full grains are complete, no disease and pests harm, go mouldy with mechanical damage fresh three Then seven materials are cleaned as raw material, cleaned, and shady place is dried is broken into fresh of 40 purposes after the moisture content of surface with pulverizer Grain；
S2, the fresh particulate samples of 40 mesh of fresh pseudo-ginseng crushed are put in the molten device of extraction, are added complex enzyme and are carried out enzymolysis processing 6h, PH 4.5, microwave radiation technology enzymolysis temperature is 70 DEG C, microwave power 580W, and complex enzyme is by cellulase 20U/g (crude drug), fruit Glue enzyme 160U/g (crude drug) is formed, and 90% ethanol for adding 10 times of amounts carries out refluxing extraction, and filtering, collects filtrate, spare；
Ethanol is recovered under reduced pressure in S3, concentration, 0.08 pa of vacuum, and temperature 70 C, obtains fresh pseudo-ginseng extracting solution；
S4, reflux decoloration 3Omin, filters, cools down, fresh pseudo-ginseng extracting solution is by being covered with the leakage of a small amount of neutral alumina while hot Bucket, is recovered under reduced pressure ethanol eluate, spare；
S5, for fresh pseudo-ginseng extracting solution by the large pore resin absorption column of pretreatment, resin demand is 1.8 times of raw material, is first used Water elution is distilled, it is colourless to be washed till efflux, decolourizes by cation exchange resin column, uses 80% ethanol Xian instead and take off liquid, uses TLC checks elution terminal, and ethanol eluate adds 1% activated carbon (by the gauge that feeds intake), collects efflux, is concentrated to dryness, to obtain the final product Arasaponin.
Specifically, the rotating speed of the pulverizer is 300r/min, and the bottom discharge mouth bottom of pulverizer is connected with Fine mesh screen.
Specifically, strong acidic ion resin or acidulous cation resin can be selected in the cation exchange resin.
Specifically, the inspissator in the S3 includes inspissator, condenser, vapor-liquid separation using vacuum-concentrcted device Device, cooler, by five component of liquid bucket, inspissator is jacket structured, and condenser is shell and tube, and cooler is coiled structure, if It is standby to use stainless steel making with material contact portion, there is good corrosion resistance.
Specifically, the top cross-sectional diameter of funnel is more than the cross-sectional diameter of its bottom in the S4.
For the present invention, using fresh pseudo-ginseng as raw material, recovery rate is not less than corresponding dry pseudo-ginseng raw material, and comprehensive cost is low In extracting notoginsenoside by raw material of dry pseudo-ginseng, the cycle is short, and cost is low, suitable for industrialized production, meanwhile, exploitation is with fresh Pseudo-ginseng extracts the deep process technology of saponin(e for raw material, and local enterprise can be made to give full play to using place of production advantage, be directly involved pseudo-ginseng The middle reaches of industrial chain, improve added value of product；The present invention using microwave radiation technology enzymolysis to extract arasaponin, with other extractions Mode is compared, and substantially reduces the test period, meanwhile, microwave is to saponin(e leaching rate in pseudo-ginseng at least without microwave treatment 4 times, extraction rate is greatly improved.
Similarly, it will be appreciated that in order to simplify the disclosure and help to understand one or more of each inventive aspect, Above in the description to the exemplary embodiment of the present invention, each feature of the invention is grouped together into single embodiment sometimes Or in descriptions thereof.However, it is as follows that the method for the disclosure should not be construed to reflection：That is the present invention for required protection It is required that than the feature more features being expressly recited in each claim.More precisely, as the following claims As reflecting, inventive aspect is all features less than single embodiment disclosed above.Therefore, it then follows specific implementation Thus claims of mode are expressly incorporated in the embodiment, wherein each claim is in itself as the present invention Separate embodiments.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of changes, modification, replace And modification, the scope of the present invention is defined by the appended.

Claims (5)

  1. A kind of 1. method for extracting arasaponin, it is characterised in that：Include the following steps：
    S1, selects maturity good, bright in colour, full grains are complete, no disease and pests harm, the fresh pseudo-ginseng material to go mouldy with mechanical damage Material be used as raw material, is then cleaned, cleaning, and shady place is dried is broken into fresh of 20-40 purposes after the moisture content of surface with pulverizer Grain；
    S2, the fresh fresh particulate samples of pseudo-ginseng 20-40 mesh crushed are put in the molten device of extraction, are added complex enzyme and are carried out enzymolysis processing 4- 6h, PH 4.5, microwave radiation technology enzymolysis temperature is 50-70 DEG C, and microwave power 500-580W, complex enzyme is by cellulase 10-20U/ G (crude drug), pectase 120-160U/g (crude drug) are formed, and are added the 6-10 times of 50%-90% measured ethanol and are carried out refluxing extraction, Filtering, collects filtrate, spare；
    Ethanol is recovered under reduced pressure in S3, concentration, vacuum 0.075-0.08 pas, and 60-70 DEG C of temperature, obtains fresh pseudo-ginseng extracting solution；
    S4, reflux decoloration 10-3Omin, filters, cools down, fresh pseudo-ginseng extracting solution is by being covered with the leakage of a small amount of neutral alumina while hot Bucket, is recovered under reduced pressure ethanol eluate, spare；
    S5, for fresh pseudo-ginseng extracting solution by the large pore resin absorption column of pretreatment, resin demand is 1.2-1.8 times of raw material, is first used Water elution is distilled, it is colourless to be washed till efflux, decolourizes by cation exchange resin column, uses 60%-80% ethanol Xian instead and take off Liquid, elution terminal is checked with TLC, and ethanol eluate adds 1% activated carbon (by the gauge that feeds intake), collects efflux, is concentrated to dryness, Up to arasaponin.
  2. A kind of 2. method for extracting arasaponin according to claim 1, it is characterised in that：The rotating speed of the pulverizer Bottom discharge mouth bottom for 100-300r/min, and pulverizer is connected with fine mesh screen.
  3. A kind of 3. method for extracting arasaponin according to claim 1, it is characterised in that：The cation exchange tree Strong acidic ion resin or acidulous cation resin can be selected in fat.
  4. A kind of 4. method for extracting arasaponin according to claim 1, it is characterised in that：Inspissator in the S3 Using vacuum-concentrcted device include inspissator, condenser, vapour liquid separator, cooler, by five component of liquid bucket, inspissator is folder Nested structure, condenser are shell and tube, and cooler is coiled structure, and equipment uses stainless steel making with material contact portion, has There is good corrosion resistance.
  5. A kind of 5. method for extracting arasaponin according to claim 1, it is characterised in that：The top of funnel in the S4 Portion's cross-sectional diameter is more than the cross-sectional diameter of its bottom.
CN201711269145.1A 2017-12-05 2017-12-05 A kind of method for extracting arasaponin Pending CN108014154A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108938696A (en) * 2018-09-13 2018-12-07 四川奇格曼药业有限公司 A kind of extracting method of arasaponin
CN109674837A (en) * 2019-02-28 2019-04-26 广南亿安食品有限公司 A kind of Radix Notoginseng medicinal extract extraction process
CN110693930A (en) * 2019-10-31 2020-01-17 江苏红豆杉药业有限公司 Method for extracting high-purity panax notoginseng saponins
CN111096990A (en) * 2020-02-19 2020-05-05 昆明邦宇制药有限公司 Method for extracting panax notoginseng saponins from fresh panax notoginseng
CN111297928A (en) * 2020-04-22 2020-06-19 广东一力罗定制药有限公司 Method for extracting panax notoginseng saponins

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Publication number Priority date Publication date Assignee Title
CN1919222A (en) * 2005-08-24 2007-02-28 天津天士力现代中药研究开发有限公司 Process for preparing notoginsen triterpenes
CN103933092A (en) * 2014-04-10 2014-07-23 文山学院文山三七研究院 Method for extracting panax notoginseng saponins from fresh panax notoginseng by complex enzyme
CN104415070A (en) * 2013-08-30 2015-03-18 河北以岭医药研究院有限公司 Method for separating and purifying total ginsenoside from ginseng

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1919222A (en) * 2005-08-24 2007-02-28 天津天士力现代中药研究开发有限公司 Process for preparing notoginsen triterpenes
CN104415070A (en) * 2013-08-30 2015-03-18 河北以岭医药研究院有限公司 Method for separating and purifying total ginsenoside from ginseng
CN103933092A (en) * 2014-04-10 2014-07-23 文山学院文山三七研究院 Method for extracting panax notoginseng saponins from fresh panax notoginseng by complex enzyme

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108938696A (en) * 2018-09-13 2018-12-07 四川奇格曼药业有限公司 A kind of extracting method of arasaponin
CN109674837A (en) * 2019-02-28 2019-04-26 广南亿安食品有限公司 A kind of Radix Notoginseng medicinal extract extraction process
CN110693930A (en) * 2019-10-31 2020-01-17 江苏红豆杉药业有限公司 Method for extracting high-purity panax notoginseng saponins
CN110693930B (en) * 2019-10-31 2021-09-17 无锡紫杉药业有限公司 Method for extracting high-purity panax notoginseng saponins
CN111096990A (en) * 2020-02-19 2020-05-05 昆明邦宇制药有限公司 Method for extracting panax notoginseng saponins from fresh panax notoginseng
CN111297928A (en) * 2020-04-22 2020-06-19 广东一力罗定制药有限公司 Method for extracting panax notoginseng saponins
CN111297928B (en) * 2020-04-22 2021-10-22 一力制药(罗定)有限公司 Method for extracting panax notoginseng saponins

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