CN104906165A - Method for extracting platycodin - Google Patents

Method for extracting platycodin Download PDF

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Publication number
CN104906165A
CN104906165A CN201510263738.1A CN201510263738A CN104906165A CN 104906165 A CN104906165 A CN 104906165A CN 201510263738 A CN201510263738 A CN 201510263738A CN 104906165 A CN104906165 A CN 104906165A
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Prior art keywords
kikyosaponin
water
extracting method
ethanol
enzymolysis
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CN201510263738.1A
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Chinese (zh)
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刘东锋
杨成东
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Nanjing Zelang Medical Technology Co Ltd
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Nanjing Zelang Medical Technology Co Ltd
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Priority to CN201510263738.1A priority Critical patent/CN104906165A/en
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Abstract

The invention discloses a method for extracting platycodin. The method comprises the following steps that firstly, the platycodon grandiflorum is dried, crushed and screened; secondly, a certain amount of platycodon grandiflorum powder is weighed and wetted through added water, and bio-enzyme reagent with the weight accounting for 0.5%-2% of the weight of the platycodon grandiflorum powder is added for carrying out enzymolysis processing; thirdly, after enzymolysis is finished, the ethanol water is added for reflux extraction, and the extraction solution is obtained; fourthly, the extraction solution is filtered, filter residues are repeatedly extracted, the extraction solution is combined, and vacuum concentration is carried out so that the extract can be formed; fifthly, the extract is dissolved, the dissolved solution is eluted through a macroporous resin column, the eluant is collected for vacuum concentration and drying, and then the platycodin is obtained. By means of the method for preparing the platycodin, the technology is easy to operate, the production cost is low, and the purity of obtained products is high.

Description

A kind of extracting method of kikyosaponin
The invention belongs to biological technical field, relate to that a kind of method that biological enzymolysis technology combines with macroporous adsorbent resin is extracted, purification kikyosaponin.
Background technology
Radix Platycodonis (formal name used at school: Platycodon grandiflorus, English: Balloon Flower), another name burden flower, Radix Platycodonis, mitral flower, campanulaceae Platycodon grandiflouorum plant, perennial herb, the avette or ovum shape lanceolar of leaf, flower skipper or dark violet white, can do ornamental flower; The Koreans are used as wild herbs and eat; Its root can be used as medicine, and has the effect such as eliminating phlegm and stopping cough, lung qi dispersing, evacuation of pus, traditional Chinese medical science common medicine.
Chemical composition contained by Radix Platycodonis is: root is containing multiple Saponin, and got 18 kinds of triterpenoid saponin so far, they are, kikyosaponin (platycodin) A, C, D, D2, D3, remove celery glycosyl kikyosaponin (deapioplatycodin) D, D3, 2-O-=acetyl group kikyosaponin (2-O-acetylplatycodin) D2, 3-O-acetyl group kikyosaponin (3-O-acetylplaty-codin) D2, polygalin (polyglalacin) D, D2, 2-O-acetyl group polygalin (2-O-acetylpolygalacin) D, D2, 3-O-acetyl group polygalin (3-O-acetylpolygalacin) D, D2, Radix Platycodonis glycoside acid-A methyl ester (methyl platyconate-A), 2-O-methyl Radix Platycodonis glycoside acid-A methyl ester (methyl-2-O-methyplatyconate-A), Radix Platycodonis glycoside acid-A lactone (platyconic acid-A lactone), wherein main constituent is platycodin D, Platycodin A C D is called again 2-O-acetyl group platycodin D, platycodoside C is called again 3-O-acetyl group platycodin D.The ruscogenin that multiple mixing Saponin produces through complete hydrolysis has: platycodigenin (platycodigenin), Polygalacic acid (polygalacic acid), and a small amount of platycogenic acid (platycogenic acid) A, B, C;
Saponin (saponin) has another name called: alkali soap body, Saponin, Saponin, saponin or saponarin." saponin " one word by English name Saponin free translation, English name then comes from Latin Sapo, means soap.One class glucosides of saponin (Saponin) to be aglycon be triterpene or spirostane compounds, is mainly distributed in the higher plant of land, is also present in the marine organisms such as Asterias amurensis Lutken and Stichopus japonicus on a small quantity.The principle active component of many Chinese herbal medicine as Radix Ginseng, Radix Polygalae, Radix Platycodonis, Radix Glycyrrhizae and Radix Bupleuri etc. all contains saponin.Some saponin also has antibacterial activity or the valuable biological activity such as antipyretic, calm, anticancer.
Prove through modern study, the pharmacological action that kikyosaponin has: 1. eliminate the phlegm and antitussive effect; 2. suppress gastric secretion and antiulcer action; 3. antiinflammatory action; 4. the effect of pair blood circulation; 5. other effects, thick kikyosaponin has calmness, analgesia and refrigeration function.
At present, in China about extracting the technique document of kikyosaponin and patent is a lot, it mainly takes extraction, ultrasonic extraction, and not only extraction ratio is low for the kikyosaponin prepared of these two kinds of methods, and purity is not high.The method that the present invention adopts biological enzymolysis technology to combine with macroporous adsorbent resin is extracted, purification kikyosaponin, can address these problems well.
Summary of the invention
The object of the invention is the technical deficiency in order to make up existing extraction kikyosaponin and defect, provide a kind of simple to operate, extraction efficiency is high, gained purity is high, pollute the extracting method of little kikyosaponin.
To achieve these goals, the present invention is by the following technical solutions:
1) Radix Platycodonis is dried, pulverize, cross 20 ~ 60 mesh sieves;
2) take a certain amount of Platycodon Root and add water infiltration, the enzyme then adding Platycodon Root weight 0.5%-2% carries out enzymolysis processing;
3) in step (2) gained material, add ethanol water reflux, extract, obtain extracting solution;
4) by extracting liquid filtering, filtering residue repeats to extract 2-3 time, merge extractive liquid, and concentrating under reduced pressure becomes extractum shape;
5) by gained extractum water dissolution in step (4), and by macroporous adsorptive resins on lysate, 60% ~ 70% ethanol elution, collect eluent concentrating under reduced pressure, dry, obtain kikyosaponin.
Step 2) described in powder and the mass volume ratio of water be 1:0.5-1, soak time is 20-60 minute.
Step 2) described in enzyme be one in cellulase, amylase, hemicellulase and pectase, temperature during enzymolysis is set in 30-45 DEG C, and enzymolysis time is 2-4 hour.
Ethanol water concentration described in step 3) is 50%-70%, and volume is 3-6 times of powder weight.
Macroporous adsorbent resin described in step 5) is the one in X-5, AB-8, NK-2, NKA-2, NK-9, D3520, D101, WLD.
Described in step 5), elution process is for first to use 2 ~ 4 times of column volume water sweetening offs, then uses 2 ~ 5 times of column volumes, 10% ~ 20% ethanol washes away partial impurities, finally use 2 ~ 5 times of column volumes, 60% ~ 70% ethanol elution, collect eluent.
This method is adopted to produce kikyosaponin, utilize the method that biological enzymolysis technology combines with macroporous adsorbent resin, abundant destruction cell wall, increase and accelerate the outflow of active component, by macroporous adsorptive resins purge process, not only substantially increase the purity of kikyosaponin, and macroporous adsorbent resin can recycling, thus reduce production cost.This simple process is easy to operate, extraction efficiency is high, it is little to pollute, products obtained therefrom purity is high, be easy to realize suitability for industrialized production.
Further illustrate the present invention below in conjunction with detailed description of the invention, but the scope of protection of present invention is not limited to following embodiments.
detailed description of the invention:
Embodiment 1:
Get fresh Radix Platycodonis to dry, pulverize, cross 20 mesh sieves.Take Platycodon Root 50 grams and put into extractor, add the water soaking 20 minutes of 25 milliliters, then the pectase reagent of 0.25 gram is added, it is enzymolysis 2 minutes under the condition of 30 DEG C in temperature, collect enzymolysis raw material, then the ethanol water reflux, extract, of 250 milliliter 60% is added, filtering residue extracts 2 times, merge 3 filtrates, concentrating under reduced pressure becomes extractum, gained extractum is dissolved, and lysate is poured in X-5 macroporous adsorptive resins, first use 2 times of column volume water sweetening offs, then 2 times of column volumes are used, 10% ethanol washes away partial impurities, finally use 2 times of column volumes, 60% ethanol elution, collect ethanol elution concentrating under reduced pressure, dry, obtain total saponins.The content of total saponins is 88.53% after testing, and the extraction ratio of total saponins is 57.5%.
Embodiment 2:
Get fresh Radix Platycodonis to dry, pulverize, cross 40 mesh sieves.Take Platycodon Root 50 grams and put into extractor, add the water soaking 40 minutes of 30 milliliters, then the pectase of 0.5 gram is added, it is enzymolysis 3 minutes under the condition of 40 DEG C in temperature, collect enzymolysis raw material, then the ethanol water reflux, extract, of 250 milliliter 60% is added, filtering residue extracts 2 times, merge 3 filtrates, concentrating under reduced pressure concentrating under reduced pressure becomes extractum, gained extractum is dissolved, and lysate is poured in D3520 macroporous adsorptive resins, first use 3 times of column volume water sweetening offs, then 4 times of column volumes are used, 15% ethanol washes away partial impurities, finally use 4 times of column volumes, the ethanol elution of 65%, collect ethanol elution concentrating under reduced pressure, dry, obtain total saponins.The content of total saponins is 89.13% after testing, and the extraction ratio of total saponins is 60.52%.
Embodiment 3:
Get fresh Radix Platycodonis to dry, pulverize, cross 60 mesh sieves.Take Platycodon Root 50 grams and put into extractor, add the water soaking 60 minutes of 50 milliliters, then the enzyme reagent of 1g gram is added, it is enzymolysis 4 minutes under the condition of 45 DEG C in temperature, collect enzymolysis raw material, then the ethanol water reflux, extract, of 250 milliliter 60% is added, filtering residue extracts 3 times, merge 4 filtrates, concentrating under reduced pressure becomes extractum, gained extractum is dissolved, and lysate is poured in AB-8 macroporous adsorptive resins, first use 4 times of column volume water sweetening offs, then 5 times of column volumes are used, 20% ethanol washes away partial impurities, finally use 5 times of column volumes, the ethanol elution of 70%, collect ethanol elution concentrating under reduced pressure, dry, obtain total saponins.The content of total saponins is 90.55% after testing, and the extraction ratio of total saponins is 61.45%.

Claims (6)

1. an extracting method for kikyosaponin, is characterized in that adopting following steps:
1) Radix Platycodonis is dried, pulverize, cross 20 ~ 60 mesh sieves;
2) take a certain amount of Platycodon Root and add water infiltration, the enzyme then adding Platycodon Root weight 0.5%-2% carries out enzymolysis processing;
3) in step (2) gained material, add ethanol water reflux, extract, obtain extracting solution;
4) by extracting liquid filtering, filtering residue repeats to extract 2-3 time, merge extractive liquid, and concentrating under reduced pressure becomes extractum shape;
5) by gained extractum water dissolution in step (4), and by macroporous adsorptive resins on lysate, 60% ~ 70% ethanol elution, collect eluent concentrating under reduced pressure, dry, obtain kikyosaponin.
2. the extracting method of kikyosaponin according to claim 1, is characterized in that: step 2) described in powder and the mass volume ratio of water be 1:0.5-1, soak time is 20-60 minute.
3. the extracting method of kikyosaponin according to claim 1, it is characterized in that: step 2) described in enzyme be one in cellulase, amylase, hemicellulase and pectase, temperature during enzymolysis is set in 30-45 DEG C, and enzymolysis time is 2-4 hour.
4. the extracting method of kikyosaponin according to claim 1, is characterized in that: the ethanol water concentration described in step 3) is 50%-70%, and volume is 3-6 times of powder weight.
5. the extracting method of kikyosaponin according to claim 1, is characterized in that: the macroporous adsorbent resin described in step 5) is the one in X-5, AB-8, NK-2, NKA-2, NK-9, D3520, D101, WLD.
6. the extracting method of kikyosaponin according to claim 1, it is characterized in that: described in step 5), elution process is for first to use 2 ~ 4 times of column volume water sweetening offs, then use 2 ~ 5 times of column volumes, 10% ~ 20% ethanol washes away partial impurities, finally use 2 ~ 5 times of column volumes, 60% ~ 70% ethanol elution, collect eluent.
CN201510263738.1A 2015-05-22 2015-05-22 Method for extracting platycodin Pending CN104906165A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108409182A (en) * 2018-03-27 2018-08-17 刘群 High-effective concrete air entraining agent, preparation method and applications
CN108929361A (en) * 2018-08-30 2018-12-04 佛山市欧若拉生物科技有限公司 A kind of processing technology of platycodin extract
CN109053825A (en) * 2018-08-30 2018-12-21 佛山市欧若拉生物科技有限公司 A kind of platycodin preparation method
KR101976999B1 (en) * 2018-10-18 2019-05-10 한국수목원관리원 The method for preparing a Platycodon grandiflorum extract using a complex enzyme and a composition for improving antioxidant, whitening or wrinkles containing the same
KR101976998B1 (en) * 2018-10-18 2019-05-10 한국수목원관리원 The method for preparing a Platycodon grandiflorum extract using a high pressure enzyme treatment and a composition having antioxidant or α-glucosidase inhibiting activity

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108409182A (en) * 2018-03-27 2018-08-17 刘群 High-effective concrete air entraining agent, preparation method and applications
CN108929361A (en) * 2018-08-30 2018-12-04 佛山市欧若拉生物科技有限公司 A kind of processing technology of platycodin extract
CN109053825A (en) * 2018-08-30 2018-12-21 佛山市欧若拉生物科技有限公司 A kind of platycodin preparation method
KR101976999B1 (en) * 2018-10-18 2019-05-10 한국수목원관리원 The method for preparing a Platycodon grandiflorum extract using a complex enzyme and a composition for improving antioxidant, whitening or wrinkles containing the same
KR101976998B1 (en) * 2018-10-18 2019-05-10 한국수목원관리원 The method for preparing a Platycodon grandiflorum extract using a high pressure enzyme treatment and a composition having antioxidant or α-glucosidase inhibiting activity

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