CN108653359A - A kind of preparation method of reed wormwood artemisia general flavone - Google Patents
A kind of preparation method of reed wormwood artemisia general flavone Download PDFInfo
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- CN108653359A CN108653359A CN201710212638.5A CN201710212638A CN108653359A CN 108653359 A CN108653359 A CN 108653359A CN 201710212638 A CN201710212638 A CN 201710212638A CN 108653359 A CN108653359 A CN 108653359A
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- Prior art keywords
- wormwood artemisia
- reed wormwood
- general flavone
- raw material
- preparation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
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- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Abstract
The present invention relates to a kind of preparation methods of reed wormwood artemisia general flavone, and in particular to a method of reed wormwood artemisia general flavone is extracted using homogenate ultrasonic method.Its processing step includes：Reed wormwood artemisia is dried in the shade, is crushed, 50 ~ 60% ethyl alcohol of 1 ~ 2 times of raw material is added, is placed in refiner and mixes homogenate；After homogenate, material is placed in ultrasonic unit, 50 ~ 60% ethanol solution of 3 ~ 5 times of raw material is added, carries out 90 ~ 120min of ultrasonic extraction, is concentrated under reduced pressure, ethyl alcohol is recycled, obtains concentrate；By gained concentrate through 80 ~ 100 mesh polyamide upper props, elution, eluent, concentration, dry, acquisition reed wormwood artemisia general flavone are collected.The present invention has many advantages, such as that quick, efficient, solvent usage amount is few, and favorable reproducibility, resin are reusable.
Description
Technical field
The present invention relates to a kind of preparation methods of reed wormwood artemisia general flavone, belong to traditional Chinese medicine extyaction field.
Background technology
Reed wormwood artemisia, nickname artemisia selengensis, water wormwood artemisia, Artemisia integrifolia L, donkey wormwood artemisia, artemisia selengensis, fragrant Chinese mugwort, little Ai, water Chinese mugwort, herbaceos perennial are planted
Strain tool faint scent.Main root unobvious are slightly apparent, have most lateral roots and fibrous fibrous root；Root-like stock is slightly thick, upright or oblique
On, 4~10 millimeters of diameter has creeping underground stem.The river and lake banks and marshland for being born in low altitude area more, all over China
There is cultivation.There is abundant edible, medical value.
The research trend of flavone compound is more related to answering for extraction process in the exploitation of its medical value in recent years
It is prepared with research, the assay of flavone compound and preparation.
Solvent extraction and microwave cooperating extraction method are mostly used to the extraction of reed wormwood artemisia general flavone at present.In solvent extraction with
Ethanol extraction method efficiency highest, but product composition is complicated, time-consuming；Microwave method is energy saving, the time shortens dramatically, but the microwave revealed is more
It may cause environmental pollution.
Homogenate extraction refers to tissue carries out tissue homogenate or defibrination by the way that extractant is added, with extract in tissue effectively at
A kind of extracting method divided.Effective ingredients in plant is extracted using homogenate method, directly material can be placed in refiner, with extraction
Solvent mixes homogenate in homogenization apparatus, and material is torn and crushed by machinery and aquashear effect, keep material broken and
The extraction of active ingredient, which synchronizes, to be carried out, and achievees the purpose that, reinforcing extraction quick to effective ingredients in plant.
In conjunction with the cavitation effect and mechanical effect of ultrasonic wave, to make the active ingredient in cell be released directly
It contacts and is dissolved wherein with solvent, to improve the recovery rate of active ingredient.
Invention content
The present invention be directed to the deficiencies in the prior art, it is desirable to provide a kind of high efficiency and selective, easy to operate, yield
It is high, by-product is few, can realization of industrialization preparation reed wormwood artemisia general flavone method.
Technical scheme of the present invention is carried out according to the following steps：
A. reed wormwood artemisia is dried in the shade, is crushed, 50 ~ 60% ethyl alcohol of 1 ~ 2 times of raw material is added, be placed in mixing homogenate 15min in refiner；
B. after being homogenized, material is placed in ultrasonic unit, 50 ~ 60% ethanol solution of 3 ~ 5 times of raw material is added, carries out ultrasound
90 ~ 120min is extracted, is concentrated under reduced pressure, ethyl alcohol is recycled, obtains concentrate；
C. by the polyamide resin column of 80 ~ 100 mesh on gained concentrate, 60 ~ 70% elution through 4 ~ 6 times of resin volumes is collected
Eluent, concentration, dry, acquisition reed wormwood artemisia general flavone.
Homogenate acc power described in step a is 200W, and homogenate rotating speed is 10000 ~ 20000r/min.
The condition of ultrasonic extraction described in step b is：Frequency 40KHz, 50 ~ 70 DEG C of ultrasonic temperature.
The mass ratio of resin demand and raw material described in step c is 1:1.
The advantage of the invention is that：
1. the present invention handles raw material using plant homogenates extraction method, crushing, extraction one step completion are realized, simultaneously because eliminating
Heating, so as to effectively avoid the destruction of general flavone ingredient.
2. the present invention uses homogenate-ultrasonic extraction reed wormwood artemisia general flavone, low, quick, the efficient, recovery rate with production cost
High advantage.
It is further illustrated the present invention below in conjunction with specific implementation mode, but the scope of protection of present invention is not limited to
In following embodiments.
Specific implementation mode：
Embodiment 1：
Reed wormwood artemisia is dried in the shade, is crushed, 50% ethyl alcohol of 1 times of raw material is added, is placed in refiner, in the item of 200W, 10000r/min
Mixing homogenate 15min under part；After homogenate, material is placed in the ultrasonic unit of frequency 40KHz, the 50% of 3 times of raw material is added
Ethanol solution carries out ultrasonic extraction 90min under conditions of 50 DEG C, is concentrated under reduced pressure, and recycles ethyl alcohol, obtains concentrate；Gained is dense
Contracting liquid is added with the polyamide resin columns of 80 mesh of quality such as raw material, and elution is collected in 70% elution through 4 times of resin volumes
Liquid, concentration, drying, obtains reed wormwood artemisia general flavone, is detected through UV, and the content of general flavone is 78.9%.
Embodiment 2：
Reed wormwood artemisia is dried in the shade, is crushed, 60% ethyl alcohol of 2 times of raw material is added, is placed in refiner, in the item of 200W, 15000r/min
Mixing homogenate 15min under part；After homogenate, material is placed in the ultrasonic unit of frequency 40KHz, the 60% of 5 times of raw material is added
Ethanol solution carries out ultrasonic extraction 100min under conditions of 60 DEG C, is concentrated under reduced pressure, and recycles ethyl alcohol, obtains concentrate；Gained is dense
Contracting liquid is added with the polyamide resin columns of 100 mesh of quality such as raw material, and elution is collected in 60% elution through 5 times of resin volumes
Liquid, concentration, drying, obtains reed wormwood artemisia general flavone, is detected through UV, and the content of general flavone is 80.5%.
Embodiment 3：
Reed wormwood artemisia is dried in the shade, is crushed, 55% ethyl alcohol of 2 times of raw material is added, is placed in refiner, in the item of 200W, 20000r/min
Mixing homogenate 15min under part；After homogenate, material is placed in the ultrasonic unit of frequency 40KHz, the 55% of 4 times of raw material is added
Ethanol solution carries out ultrasonic extraction 120min under conditions of 70 DEG C, is concentrated under reduced pressure, and recycles ethyl alcohol, obtains concentrate；Gained is dense
Contracting liquid is added with the polyamide resin columns of 100 mesh of quality such as raw material, and elution is collected in 65% elution through 6 times of resin volumes
Liquid, concentration, drying, obtains reed wormwood artemisia general flavone, is detected through UV, and the content of general flavone is 82.2%.
Claims (4)
1. a kind of preparation method of reed wormwood artemisia general flavone, it is characterised in that：
A. reed wormwood artemisia is dried in the shade, is crushed, 50 ~ 60% ethyl alcohol of 1 ~ 2 times of raw material is added, be placed in mixing homogenate 15min in refiner；
B. after being homogenized, material is placed in ultrasonic unit, 50 ~ 60% ethanol solution of 3 ~ 5 times of raw material is added, carries out ultrasound
90 ~ 120min is extracted, is concentrated under reduced pressure, ethyl alcohol is recycled, obtains concentrate；
C. by the polyamide resin column of 80 ~ 100 mesh on gained concentrate, 60 ~ 70% elution through 4 ~ 6 times of resin volumes is collected
Eluent, concentration, dry, acquisition reed wormwood artemisia general flavone.
2. a kind of preparation method of reed wormwood artemisia general flavone according to claim 1, which is characterized in that even described in step a
Pulp grinder power is 200W, and homogenate rotating speed is 10000 ~ 20000r/min.
3. a kind of preparation method of reed wormwood artemisia general flavone according to claim 1, which is characterized in that super described in step b
Sound extraction condition be：Frequency 40KHz, 50 ~ 70 DEG C of ultrasonic temperature.
4. a kind of preparation method of reed wormwood artemisia general flavone according to claim 1, which is characterized in that the tree described in step c
The mass ratio of fat dosage and raw material is 1:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710212638.5A CN108653359A (en) | 2017-04-01 | 2017-04-01 | A kind of preparation method of reed wormwood artemisia general flavone |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710212638.5A CN108653359A (en) | 2017-04-01 | 2017-04-01 | A kind of preparation method of reed wormwood artemisia general flavone |
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| CN108653359A true CN108653359A (en) | 2018-10-16 |
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| CN201710212638.5A Pending CN108653359A (en) | 2017-04-01 | 2017-04-01 | A kind of preparation method of reed wormwood artemisia general flavone |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111514176A (en) * | 2020-04-28 | 2020-08-11 | 江汉大学 | Application of artemisia selengensis polyphenol extract in oral mucosa repair |
| CN112143459A (en) * | 2020-07-06 | 2020-12-29 | 江西师范大学 | Ultraviolet absorbent and preparation method thereof |
-
2017
- 2017-04-01 CN CN201710212638.5A patent/CN108653359A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111514176A (en) * | 2020-04-28 | 2020-08-11 | 江汉大学 | Application of artemisia selengensis polyphenol extract in oral mucosa repair |
| CN112143459A (en) * | 2020-07-06 | 2020-12-29 | 江西师范大学 | Ultraviolet absorbent and preparation method thereof |
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Application publication date: 20181016 |