CN109646480A - A kind of preparation method of total flavonoids in albizia julibrissin durazz - Google Patents
A kind of preparation method of total flavonoids in albizia julibrissin durazz Download PDFInfo
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- CN109646480A CN109646480A CN201710932306.4A CN201710932306A CN109646480A CN 109646480 A CN109646480 A CN 109646480A CN 201710932306 A CN201710932306 A CN 201710932306A CN 109646480 A CN109646480 A CN 109646480A
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- Prior art keywords
- total flavonoids
- albizia julibrissin
- julibrissin durazz
- raw material
- homogenate
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The present invention relates to a kind of preparation methods of total flavonoids in albizia julibrissin durazz, and in particular to a method of total flavonoids in albizia julibrissin durazz is extracted using homogenate-ultrasonic method.Its processing step includes: that Flos Albiziae is dried in the shade, and is crushed, and 1~2 times of raw material of 50~60% ethyl alcohol is added, and is placed in refiner and mixes homogenate；After homogenate, material is placed in ultrasonic unit, 3~5 times of raw material of 50~60% ethanol solution is added, carries out 90~120min of ultrasonic extraction, is concentrated under reduced pressure, ethyl alcohol is recycled, obtains concentrate；By gained concentrate through 80~100 mesh polyamide upper props, elution, eluent, concentration, dry, acquisition total flavonoids in albizia julibrissin durazz are collected.The present invention has many advantages, such as that quick, efficient, solvent usage amount is few, and favorable reproducibility, resin are reusable.
Description
Technical field
The present invention relates to a kind of preparation methods of total flavonoids in albizia julibrissin durazz, belong to traditional Chinese medicine extyaction field.
Background technique
Flos Albiziae is the drying inflorescence of leguminous plant silk tree, originates in Hebei, Henan, Shaanxi, Zhejiang, Jiangsu, Shandong, peace
The ground such as emblem, Hubei are a kind of plants of integration of drinking and medicinal herbs.Flos Albiziae has the function of resolving stagnation for tranquilization, is frequently used for confused and worried, sorrow
The symptoms such as strongly fragrant insomnia, pent-up is uncomfortable in chest, amnesia, acute conjunctivitis.Pharmacological evaluation shows that Flos Albiziae decocting liquid and alcohol extracting thing are shown
Significant antidepressant effect.Contain polyphenol, flavones, polysaccharide and other multiple active components in Flos Albiziae, silk tree flower polyphenol is to nitrous acid
Salt has scavenging effect, and flavones has certain neuroprotection, wherein the quercitin to play a major role.
Flavone compound refers to that basic parent nucleus is the series compound of 2- phenyl chromone, is widely present in plant kingdom,
Many medium-height grass the effective elements of the medicines, it is many kinds of, have multiple biological activities, except with antibacterial, anti-inflammatory, it is disease-resistant become, decompression,
Outside the effects of clearing heat and detoxicating, calm, diuresis, anti-oxidant, anticancer, anti-cancer, inhibit in terms of also have remarkable result, be
A kind of important natural organic oxidation-resistant agent with development prospect.The research trend of flavone compound is medicinal in its in recent years
The exploitation of value is more related to the application study of extraction process, the recovery rate measurement of flavone compound and preparation preparation.
Solvent extraction and microwave cooperating extraction method are mostly used to the extraction of total flavonoids in albizia julibrissin durazz at present.In solvent extraction
With ethanol extraction method efficiency highest, but product composition is complicated, time-consuming；The microwave that microwave method energy conservation, time shorten dramatically, but reveals
It is more likely to cause environmental pollution.
Homogenate, which is extracted, refers to that tissue carries out tissue homogenate or defibrination by addition extractant, with extract in tissue effectively at
A kind of extracting method divided.Effective ingredients in plant is extracted using homogenate method, directly material can be placed in refiner, with extraction
Solvent mixes homogenate in homogenization apparatus, and material is torn and crushed by the effect of mechanical and aquashear, keep material broken and
The extraction of effective component is synchronous to be carried out, and achievees the purpose that, reinforcing quick to effective ingredients in plant is extracted.
In conjunction with the cavitation effect and mechanical effect of ultrasonic wave, so that the effective component in cell be made to be released directly
It contacts and is dissolved wherein with solvent, to improve the recovery rate of effective component.
Summary of the invention
The present invention be directed to the deficiencies in the prior art, it is desirable to provide a kind of high efficiency and selective, easy to operate, yield
It is high, by-product is few, can realization of industrialization the method for preparing total flavonoids in albizia julibrissin durazz.
Technical solution of the present invention is carried out according to the following steps:
A. Flos Albiziae is dried in the shade, is crushed, 1~2 times of raw material of 50~60% ethyl alcohol is added, be placed in refiner and mix homogenate
15min；
B. after being homogenized, material is placed in ultrasonic unit, 3~5 times of raw material of 50~60% ethanol solution is added, is surpassed
Sound extracts 90~120min, is concentrated under reduced pressure, and recycles ethyl alcohol, obtains concentrate；
C. by the polyamide resin column of 80~100 mesh on gained concentrate, 60~70% elution through 4~6 times of resin volumes,
Collect eluent, concentration, dry, acquisition total flavonoids in albizia julibrissin durazz.
Refiner power described in step a is 200W, and homogenate revolving speed is 10000~20000r/min.
The condition of ultrasonic extraction described in step b are as follows: frequency 40KHz, 50~70 DEG C of ultrasonic temperature.
The mass ratio of amount of resin described in step c and raw material is 1:1.
The present invention has the advantages that
1) present invention handles raw material using plant homogenates extraction method, realizes crushing, extracts step completion, simultaneously because eliminating
Heating, so as to effectively avoid the destruction of general flavone ingredient.
2) present invention uses homogenate-ultrasonic extraction total flavonoids in albizia julibrissin durazz, extraction low, quick, efficient with production cost
The high advantage of rate.
The present invention is further illustrated below in conjunction with specific embodiment, but the scope of protection of present invention is not limited to
In following embodiments.
Specific embodiment:
Embodiment 1:
Flos Albiziae is dried in the shade, is crushed, 1 times of raw material of 50% ethyl alcohol is added, is placed in refiner, in 200W, 10000r/min
Under the conditions of mixing homogenate 15min；After homogenate, material is placed in the ultrasonic unit of frequency 40KHz, the 50% of 3 times of raw material is added
Ethanol solution, under conditions of 50 DEG C carry out ultrasonic extraction 90min, be concentrated under reduced pressure, recycle ethyl alcohol, obtain concentrate；By gained
Concentrate is added with the polyamide resin columns of 80 mesh of quality such as raw material, and 70% elution through 4 times of resin volumes, collection is washed
De- liquid, concentration, drying, obtains total flavonoids in albizia julibrissin durazz, and recovery rate 92.84% is detected through UV, and the content of general flavone is 72.3%.
Embodiment 2:
Flos Albiziae is dried in the shade, is crushed, 2 times of raw material of 60% ethyl alcohol is added, is placed in refiner, in 200W, 15000r/min
Under the conditions of mixing homogenate 15min；After homogenate, material is placed in the ultrasonic unit of frequency 40KHz, the 60% of 5 times of raw material is added
Ethanol solution, under conditions of 60 DEG C carry out ultrasonic extraction 100min, be concentrated under reduced pressure, recycle ethyl alcohol, obtain concentrate；By gained
Concentrate is added with the polyamide resin columns of 100 mesh of quality such as raw material, and 60% elution through 5 times of resin volumes, collection is washed
De- liquid, concentration, drying, obtains total flavonoids in albizia julibrissin durazz, and recovery rate 96.27% is detected through UV, and the content of general flavone is 76.2%.
Embodiment 3:
Flos Albiziae is dried in the shade, is crushed, 2 times of raw material of 55% ethyl alcohol is added, is placed in refiner, in 200W, 20000r/min
Under the conditions of mixing homogenate 15min；After homogenate, material is placed in the ultrasonic unit of frequency 40KHz, the 55% of 4 times of raw material is added
Ethanol solution, under conditions of 70 DEG C carry out ultrasonic extraction 120min, be concentrated under reduced pressure, recycle ethyl alcohol, obtain concentrate；By gained
Concentrate is added with the polyamide resin columns of 100 mesh of quality such as raw material, and 65% elution through 6 times of resin volumes, collection is washed
De- liquid, concentration, drying, obtains total flavonoids in albizia julibrissin durazz, and recovery rate 93.58% is detected through UV, and the content of general flavone is 79.4%.
Claims (4)
1. a kind of preparation method of total flavonoids in albizia julibrissin durazz, it is characterised in that:
A. Flos Albiziae is dried in the shade, is crushed, 1~2 times of raw material of 50~60% ethyl alcohol is added, be placed in refiner and mix homogenate
15min；
B. after being homogenized, material is placed in ultrasonic unit, 3~5 times of raw material of 50~60% ethanol solution is added, is surpassed
Sound extracts 90~120min, is concentrated under reduced pressure, and recycles ethyl alcohol, obtains concentrate；
C. by the polyamide resin column of 80~100 mesh on gained concentrate, 60~70% elution through 4~6 times of resin volumes,
Collect eluent, concentration, dry, acquisition total flavonoids in albizia julibrissin durazz.
2. a kind of preparation method of total flavonoids in albizia julibrissin durazz according to claim 1, which is characterized in that described in step a
Refiner power is 200W, and homogenate revolving speed is 10000~20000r/min.
3. a kind of preparation method of total flavonoids in albizia julibrissin durazz according to claim 1, which is characterized in that described in step b
The condition of ultrasonic extraction are as follows: frequency 40KHz, 50~70 DEG C of ultrasonic temperature.
4. a kind of preparation method of total flavonoids in albizia julibrissin durazz according to claim 1, which is characterized in that described in step c
The mass ratio of amount of resin and raw material is 1:1.
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| CN201710932306.4A CN109646480A (en) | 2017-10-10 | 2017-10-10 | A kind of preparation method of total flavonoids in albizia julibrissin durazz |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112870251A (en) * | 2021-01-28 | 2021-06-01 | 常熟理工学院 | Method for extracting and purifying total flavonoids of albizia julibrissin |
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2017
- 2017-10-10 CN CN201710932306.4A patent/CN109646480A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112870251A (en) * | 2021-01-28 | 2021-06-01 | 常熟理工学院 | Method for extracting and purifying total flavonoids of albizia julibrissin |
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Application publication date: 20190419 |