CN104906238A - Method for preparing herba houttuyniae total flavonoids - Google Patents
Method for preparing herba houttuyniae total flavonoids Download PDFInfo
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- CN104906238A CN104906238A CN201510270086.4A CN201510270086A CN104906238A CN 104906238 A CN104906238 A CN 104906238A CN 201510270086 A CN201510270086 A CN 201510270086A CN 104906238 A CN104906238 A CN 104906238A
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- herba houttuyniae
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- total flavones
- cordate houttuynia
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Abstract
The invention relates to a method for preparing herba houttuyniae total flavonoids, in particular to a method for extracting the herba houttuyniae total flavonoids by means of a homogenate and ultrasonic method. The method includes the steps that herba houttuyniae is dried in shade and smashed, ethyl alcohol with the concentration of 50-60% 1-2 times of the raw material is added, and the herba houttuyniae and the ethyl alcohol are placed into a homogenizer to be mixed and homogenized; after homogenating, the materials are placed in an ultrasonic device, an ethyl alcohol solution with the concentration of 50-60% 3-5 times of the raw material is added, ultrasonic extraction is carried out for 90-120 minutes, decompression condensation is carried out, the ethyl alcohol is recycled, and condensed liquid is obtained; the condensed liquid is subjected to 80-mesh to 100-mesh polyamide resin column installing and elution, eluent is collected, condensation and drying are carried out, and the herba houttuyniae total flavonoids are obtained. The method has the advantages of being quick, being efficient and only using a small amount of solvents, and is good in repeatability, and resin can be repeatedly used.
Description
Technical field
The present invention relates to a kind of preparation method of cordate houttuynia total flavones, belong to traditional Chinese medicine extyaction field.
Background technology
Herba Houttuyniae is Saururaceae Houttuynia plant Herba Houttuyniae herb, is medicine food dual purpose plant.Its nature and flavor are pungent, cold, have effect of heat-clearing and toxic substances removing, detumescence and apocenosis, inducing diuresis for treating stranguria syndrome.Herba Houttuyniae contains the flavone compounds such as Quercetin, Quercitroside, hyperin, rutin.
Flavone compound refers to that basic parent nucleus is the series compound of 2-phenyl chromone, extensively be present in plant kingdom, many medium-height grass the effective elements of the medicines, of a great variety, there is multiple biological activity, except having the effects such as antibacterial, antiinflammatory, disease-resistant change, blood pressure lowering, heat-clearing and toxic substances removing, calmness, diuresis, in antioxidation, anticancer, anti-cancer, suppressing also there is remarkable result in lipase etc., is a kind of important natural organic oxidation-resistant agent with DEVELOPMENT PROSPECT.The research trend of flavone compound is in the exploitation of its medical value in recent years, relates to the preparation of the applied research of extraction process, the assay of flavone compound and preparation more.
At present solvent extraction and microwave cooperating extraction method are adopted more to the extraction of cordate houttuynia total flavones.Most effective with ethanol extraction method in solvent extraction, but product composition is complicated, consuming time; Microwave method is energy-conservation, the time shortens dramatically, but the microwave revealed more may cause environmental pollution.
Homogenate is extracted and is referred to that tissue carries out tissue homogenate or defibrination by adding extractant, to extract a kind of extracting method of effective ingredient in tissue.Application homogenate method extracts effective ingredients in plant, can directly material be placed in refiner, in homogenization apparatus, homogenate is mixed with Extraction solvent, material torn by machinery and aquashear effect and pulverizes, material extraction that is broken and effective ingredient is synchronously carried out, reaches quick to effective ingredients in plant, strengthen the object extracted.
Again in conjunction with hyperacoustic cavitation effect and mechanical effect, thus the effective ingredient in cell is released directly be dissolved in wherein with solvent contacts, thus the extraction ratio of raising effective ingredient.
Summary of the invention
The present invention be directed to the deficiencies in the prior art, aim to provide a kind of high efficiency and selectivity, easy and simple to handle, productive rate is high, by-product is few, can the method that cordate houttuynia total flavones be prepared of realization of industrialization.
Technical scheme of the present invention is carried out according to the following step:
A. Herba Houttuyniae is dried in the shade, pulverize, add the ethanol of 50 ~ 60% of raw material 1 ~ 2 times, be placed in refiner mixing homogenate 15min;
B. after homogenate, material is placed in ultrasonic unit, adds the alcoholic solution of 50 ~ 60% of raw material 3 ~ 5 times, carry out ultrasonic extraction 90 ~ 120min, concentrating under reduced pressure, reclaim ethanol, obtain concentrated solution;
C. by 80 ~ 100 object polyamide resin columns on gained concentrated solution, through the eluting of 60 ~ 70% of 4 ~ 6 times of resin volumes, eluent is collected, concentrated, dry, obtain cordate houttuynia total flavones.
Refiner power described in step a is 200W, and homogenate rotating speed is 10000 ~ 20000r/min.
The condition of the ultrasonic extraction described in step b is: frequency 40KHz, ultrasonic temperature 50 ~ 70 DEG C.
The mass ratio of the resin demand described in step c and raw material is 1:1.
The invention has the advantages that:
1. the present invention adopts plant homogenates extraction method process raw material, achieves pulverizing, extracts a step and complete, and simultaneously owing to eliminating heating, thus effectively can avoid the destruction of total flavones composition.
2. the present invention adopts homogenate-ultrasonic extraction cordate houttuynia total flavones, has the advantage that production cost is low, quick, efficient, extraction ratio is high.
Further illustrate the present invention below in conjunction with detailed description of the invention, but the scope of protection of present invention is not limited to following embodiments.
detailed description of the invention:
Embodiment 1:
Herba Houttuyniae is dried in the shade, pulverizes, add the ethanol of 50% of raw material 1 times, be placed in refiner, under the condition of 200W, 10000r/min, mix homogenate 15min; After homogenate, material is placed in the ultrasonic unit of frequency 40KHz, adds the alcoholic solution of 50% of raw material 3 times, under the condition of 50 DEG C, carry out ultrasonic extraction 90min, concentrating under reduced pressure, reclaim ethanol, obtain concentrated solution; Gained concentrated solution is added with 80 object polyamide resin columns of the quality such as raw material, through the eluting of 70% of 4 times of resin volumes, collect eluent, concentrated, dry, obtain cordate houttuynia total flavones, extraction ratio is 92.84%, detect through UV, the content of total flavones is 77.4%.
Embodiment 2:
Herba Houttuyniae is dried in the shade, pulverizes, add the ethanol of 60% of raw material 2 times, be placed in refiner, under the condition of 200W, 15000r/min, mix homogenate 15min; After homogenate, material is placed in the ultrasonic unit of frequency 40KHz, adds the alcoholic solution of 60% of raw material 5 times, under the condition of 60 DEG C, carry out ultrasonic extraction 100min, concentrating under reduced pressure, reclaim ethanol, obtain concentrated solution; Gained concentrated solution is added with 100 object polyamide resin columns of the quality such as raw material, through the eluting of 60% of 5 times of resin volumes, collect eluent, concentrated, dry, obtain cordate houttuynia total flavones, extraction ratio is 96.27%, detect through UV, the content of total flavones is 81.5%.
Embodiment 3:
Herba Houttuyniae is dried in the shade, pulverizes, add the ethanol of 55% of raw material 2 times, be placed in refiner, under the condition of 200W, 20000r/min, mix homogenate 15min; After homogenate, material is placed in the ultrasonic unit of frequency 40KHz, adds the alcoholic solution of 55% of raw material 4 times, under the condition of 70 DEG C, carry out ultrasonic extraction 120min, concentrating under reduced pressure, reclaim ethanol, obtain concentrated solution; Gained concentrated solution is added with 100 object polyamide resin columns of the quality such as raw material, through the eluting of 65% of 6 times of resin volumes, collect eluent, concentrated, dry, obtain cordate houttuynia total flavones, extraction ratio is 93.58%, detect through UV, the content of total flavones is 83.1%.
Claims (4)
1. a preparation method for cordate houttuynia total flavones, is characterized in that:
A. Herba Houttuyniae is dried in the shade, pulverize, add the ethanol of 50 ~ 60% of raw material 1 ~ 2 times, be placed in refiner mixing homogenate 15min;
B. after homogenate, material is placed in ultrasonic unit, adds the alcoholic solution of 50 ~ 60% of raw material 3 ~ 5 times, carry out ultrasonic extraction 90 ~ 120min, concentrating under reduced pressure, reclaim ethanol, obtain concentrated solution;
C. by 80 ~ 100 object polyamide resin columns on gained concentrated solution, through the eluting of 60 ~ 70% of 4 ~ 6 times of resin volumes, eluent is collected, concentrated, dry, obtain cordate houttuynia total flavones.
2. the preparation method of a kind of cordate houttuynia total flavones according to claim 1, is characterized in that, the refiner power described in step a is 200W, and homogenate rotating speed is 10000 ~ 20000r/min.
3. the preparation method of a kind of cordate houttuynia total flavones according to claim 1, is characterized in that, the condition of the ultrasonic extraction described in step b is: frequency 40KHz, ultrasonic temperature 50 ~ 70 DEG C.
4. the preparation method of a kind of cordate houttuynia total flavones according to claim 1, is characterized in that, the mass ratio of the resin demand described in step c and raw material is 1:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510270086.4A CN104906238A (en) | 2015-05-26 | 2015-05-26 | Method for preparing herba houttuyniae total flavonoids |
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| CN201510270086.4A CN104906238A (en) | 2015-05-26 | 2015-05-26 | Method for preparing herba houttuyniae total flavonoids |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105802275A (en) * | 2016-03-25 | 2016-07-27 | 赤峰昭乌达羊绒制品有限责任公司 | Preparation method and application of herba houttuyniae natural dye |
| CN108033987A (en) * | 2017-12-28 | 2018-05-15 | 暨南大学 | A kind of method of sodium houttuyfonate heterozygosis flavones Houttuynoid A |
-
2015
- 2015-05-26 CN CN201510270086.4A patent/CN104906238A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105802275A (en) * | 2016-03-25 | 2016-07-27 | 赤峰昭乌达羊绒制品有限责任公司 | Preparation method and application of herba houttuyniae natural dye |
| CN108033987A (en) * | 2017-12-28 | 2018-05-15 | 暨南大学 | A kind of method of sodium houttuyfonate heterozygosis flavones Houttuynoid A |
| CN108033987B (en) * | 2017-12-28 | 2019-12-24 | 暨南大学 | Method for synthesizing houttuynin heterozygotic flavone Houttuynoid A |
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Application publication date: 20150916 |
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| WD01 | Invention patent application deemed withdrawn after publication |