CN102250073A - Method for directly extracting puerarin from fresh kudzuvine pueraria - Google Patents
Method for directly extracting puerarin from fresh kudzuvine pueraria Download PDFInfo
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- CN102250073A CN102250073A CN2011101328946A CN201110132894A CN102250073A CN 102250073 A CN102250073 A CN 102250073A CN 2011101328946 A CN2011101328946 A CN 2011101328946A CN 201110132894 A CN201110132894 A CN 201110132894A CN 102250073 A CN102250073 A CN 102250073A
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- kudzu vine
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Abstract
The invention discloses a method for directly extracting puerarin from fresh kudzuvine pueraria, relating to the technical field of traditional Chinese medicine extraction. The process flow comprises the following steps: cleaning, removing impurities, airing, carrying out coarse smashing, adding ethanol, soaking, carrying out high-speed shear, carrying out microwave-assisted extraction, filtering and concentrating. The process has the characteristics of low cost and high extraction ratio and the like, and is simple to operate; and in the process, the fresh kudzuvine pueraria can be used for direct extraction and dried kudzuvine pueraria can also be used for extraction, and the process can be used for extracting effective components of traditional Chinese medicines such as panax notoginseng, salvia miltiorrhiza and the like.
Description
Technical field
The present invention relates to the traditional Chinese medicine extraction technical field, particularly a kind of fresh root of kudzu vine directly extracts the method for puerarin.
Background technology
Puerarin is to extract a kind of isoflavonoid that obtains from the root of kudzu vine, has the expansion cardiovascular and cerebrovascular, reduces myocardium keto consumption, improves myocardium shrinkage function and promotes the blood microcirculation effect.Therefore, Food science man is to the very big interest of its research performance, and from solvent extraction early, advanced extractive techniques such as microwave-assisted extraction till now, ultrasonic extraction are extracted Radix Puerariae total flavones as employing microwave-assisted such as white orchid jasmines, obtain reasonable extraction effect.The extracting method of Radix Puerariae total flavones and puerarin is more, wherein involves microwave-assisted to extract productive rate higher with ultrasonic, compares with hydrolysis with the traditional technology heating and refluxing extraction, and it is with short production cycle, efficient is high, and equipment is simple, and is low in the pollution of the environment, and product cost is low.But because puerarin content is low in the root of kudzu vine, extraction yield is also lower, has hindered the development of the suitability for industrialized production of puerarin extraction.
Summary of the invention
At the deficiency that prior art exists, technical problem to be solved by this invention provides a kind of simple to operate, with low cost, puerarin extracting method that extraction yield is high.
For solving the problems of the technologies described above, the technical solution used in the present invention is the method that a kind of fresh root of kudzu vine of invention directly extracts puerarin, and its technical process is as follows:
A, the fresh root of kudzu vine is cleaned, impurity elimination, dried, carry out coarse breaking, cross 20 mesh sieves; Then, insert in the container, adding concentration is that 30-50%, consumption are root of kudzu vine total amount 15-30 ethanolic soln doubly, soaks 2min, becomes mixture, and is standby;
B, above-mentioned standby mixture joined carry out high speed shear in the high-speed shearing device, shear time is 4-8min, and velocity of shear is 4000-8000r/min;
C, will insert in the extractor through the mixture of high speed shear and extract, and in the leaching process, adopt microwave radiation to assist, microwave irradiation time 10-30s, microwave power are 300-500w; After extraction was finished, filter and remove residue stayed extracting solution standby;
D, above-mentioned standby extracting solution is condensed into the puerarin that paste is extraction.
Described concentration of ethanol is 40%.
The high speed shear time is 6min, and velocity of shear is 6000r/min.
Microwave irradiation time is 20s, and microwave power is 500w.
Described concentrating is the concentrating under reduced pressure method.
The fresh root of kudzu vine of the present invention directly extracts the method for puerarin, be with the fresh root of kudzu vine not after the direct fragmentation of drying, by high speed shear, the broken largely cell of root of kudzu vine plant is convenient to the puerarin stripping in the root of kudzu vine vegetable cell, in extraction process, adopt microwave radiation then, thereby, improved the extraction yield of puerarin greatly, reduced the use of organic solvent, saved production cost, also reduced pollution environment.Simultaneously, its technical process is relatively simple, thereby its operation is also fairly simple.
Embodiment
Below in conjunction with embodiment, to further instruction of the present invention.Following explanation is to adopt the mode that exemplifies, but protection scope of the present invention is not limited thereto.
Embodiment one:
A, get the fresh root of kudzu vine 10 grams, clean earth, removes rotten epidermis and coring, dry moisture, carry out coarse breaking (this example adopts DS-high-speed tissue mashing machine to carry out coarse breaking, also can adopt other fragmentation or smash equipment to pieces) then, 20 mesh sieves; Then as the fresh root of kudzu vine particle of coarse breaking is inserted in the container, add concentration and be 200 milliliters of 40% ethanolic solns, immersion 2min becomes mixture, and is standby;
B, above-mentioned standby mixture is joined the high-speed shearing device (high-speed shearing machine that this example adopts Shanghai Frock Fluid Machinery Manufacture Co., Ltd. to produce, also can adopt other high-speed shearing equipment) in carry out high speed shear, shear time is 4min, and velocity of shear is 6000r/min;
C, will insert in the extractor through the mixture of high speed shear and extract, in the leaching process, adopt microwave radiation to assist (this example adopts NJL07-3 type experiment special microwave oven, also can adopt other microwave equipment), microwave irradiation time 30s, microwave power are 300w; After extraction was finished, filter and remove residue stayed extracting solution standby;
D, above-mentioned standby extracting solution is adopted the concentrating under reduced pressure method, be condensed into the puerarin that paste is extraction.
The puerarin of said extracted detects (this example adopts day LC-2010AHT high performance liquid chromatograph and the TMP-2 upper utensil type electronic balance of island proper Tianjin instrument company product) through high performance liquid chromatography, and its puerarin content is 1.38mg/g.
Embodiment two:
A, get the fresh root of kudzu vine 10 grams, clean earth, removes rotten epidermis and coring, dry moisture, carry out coarse breaking (this example adopts DS-high-speed tissue mashing machine to carry out coarse breaking, also can adopt other fragmentation or smash equipment to pieces) then, 20 mesh sieves; Then as the fresh root of kudzu vine particle of coarse breaking is inserted in the container, add concentration and be 200 milliliters of 40% ethanolic solns, immersion 2min becomes mixture, and is standby;
B, above-mentioned standby mixture is joined the high-speed shearing device (high-speed shearing machine that this example adopts Shanghai Frock Fluid Machinery Manufacture Co., Ltd. to produce, also can adopt other high-speed shearing equipment) in carry out high speed shear, shear time is 2min, and velocity of shear is 6000r/min;
C, will insert in the extractor through the mixture of high speed shear and extract, in the leaching process, adopt microwave radiation to assist (this example adopts NJL07-3 type experiment special microwave oven, also can adopt other microwave equipment), microwave irradiation time 30s, microwave power are 300w; After extraction was finished, filter and remove residue stayed extracting solution standby;
D, above-mentioned standby extracting solution is adopted the concentrating under reduced pressure method, be condensed into the puerarin that paste is extraction.
The puerarin of said extracted detects (this example adopts day LC-2010AHT high performance liquid chromatograph and the TMP-2 upper utensil type electronic balance of island proper Tianjin instrument company product) through high performance liquid chromatography, and its puerarin content is 0.99mg/g.
Embodiment three:
A, get the fresh root of kudzu vine 10 grams, clean earth, removes rotten epidermis and coring, dry moisture, carry out coarse breaking (this example adopts DS-high-speed tissue mashing machine to carry out coarse breaking, also can adopt other fragmentation or smash equipment to pieces) then, 20 mesh sieves; Then as the fresh root of kudzu vine particle of coarse breaking is inserted in the container, add concentration and be 200 milliliters of 40% ethanolic solns, immersion 2min becomes mixture, and is standby;
B, above-mentioned standby mixture is joined the high-speed shearing device (high-speed shearing machine that this example adopts Shanghai Frock Fluid Machinery Manufacture Co., Ltd. to produce, also can adopt other high-speed shearing equipment) in carry out high speed shear, shear time is 6min, and velocity of shear is 6000r/min;
C, will insert in the extractor through the mixture of high speed shear and extract, in the leaching process, adopt microwave radiation to assist (this example adopts NJL07-3 type experiment special microwave oven, also can adopt other microwave equipment), microwave irradiation time 30s, microwave power are 300w; After extraction was finished, filter and remove residue stayed extracting solution standby;
D, above-mentioned standby extracting solution is adopted the concentrating under reduced pressure method, be condensed into the puerarin that paste is extraction.
The puerarin of said extracted detects (this example adopts day LC-2010AHT high performance liquid chromatograph and the TMP-2 upper utensil type electronic balance of island proper Tianjin instrument company product) through high performance liquid chromatography, and its puerarin content is 1.40mg/g.
Embodiment four:
A, get the fresh root of kudzu vine 10 grams, clean earth, removes rotten epidermis and coring, dry moisture, carry out coarse breaking (this example employing DS-high-speed tissue mashing machine carries out coarse breaking, also can adopt other fragmentation or smash equipment to pieces) then, its fineness was 20 mesh sieves; Then as the fresh root of kudzu vine particle of coarse breaking is inserted in the container, add concentration and be 200 milliliters of 40% ethanolic solns, immersion 2min becomes mixture, and is standby;
B, above-mentioned standby mixture is joined the high-speed shearing device (high-speed shearing machine that this example adopts Shanghai Frock Fluid Machinery Manufacture Co., Ltd. to produce, also can adopt other high-speed shearing equipment) in carry out high speed shear, shear time is 6min, and velocity of shear is 6000r/min;
C, will insert in the extractor through the mixture of high speed shear and extract, in the leaching process, adopt microwave radiation to assist (this example adopts NJL07-3 type experiment special microwave oven, also can adopt other microwave equipment), microwave irradiation time 20s, microwave power are 500w; After extraction was finished, filter and remove residue stayed extracting solution standby;
D, above-mentioned standby extracting solution is adopted the concentrating under reduced pressure method, be condensed into the puerarin that paste is extraction.
The puerarin of said extracted detects (this example adopts day LC-2010AHT high performance liquid chromatograph and the TMP-2 upper utensil type electronic balance of island proper Tianjin instrument company product) through high performance liquid chromatography, and its puerarin content is 2.02mg/g.
The fresh root of kudzu vine of the present invention directly extracts the method for puerarin, can directly extract with the fresh root of kudzu vine, also can extract with the dried root of kudzu vine, can also be used to extract the effective ingredient of Chinese medicines such as pseudo-ginseng, the red sage root.
Claims (5)
1. the fresh root of kudzu vine directly extracts the method for puerarin, it is characterized in that technical process is as follows:
A, the fresh root of kudzu vine is cleaned, impurity elimination, dried, carry out coarse breaking, cross 20 mesh sieves; Then, insert in the container, adding concentration is that 30-50%, consumption are root of kudzu vine total amount 15-30 ethanolic soln doubly, soaks 2min, becomes mixture, and is standby;
B, above-mentioned standby mixture joined carry out high speed shear in the high-speed shearing device, shear time is 4-8min, and velocity of shear is 4000-8000r/min;
C, will insert in the extractor through the mixture of high speed shear and extract, and in the leaching process, adopt microwave radiation to assist, microwave irradiation time 10-30s, microwave power are 300-500w; After extraction was finished, filter and remove residue stayed extracting solution standby;
D, above-mentioned standby extracting solution is condensed into the puerarin that paste is extraction.
2. the fresh root of kudzu vine according to claim 1 directly extracts the method for puerarin, it is characterized in that: described concentration of ethanol is 40%.
3. the fresh root of kudzu vine according to claim 1 and 2 directly extracts the method for puerarin, it is characterized in that: the high speed shear time is 6min, and velocity of shear is 6000r/min.
4. the fresh root of kudzu vine according to claim 3 directly extracts the method for puerarin, it is characterized in that: microwave irradiation time is 20s, and microwave power is 500w.
5. the fresh root of kudzu vine according to claim 4 directly extracts the method for puerarin, it is characterized in that: described concentrating is the concentrating under reduced pressure method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101328946A CN102250073A (en) | 2011-05-19 | 2011-05-19 | Method for directly extracting puerarin from fresh kudzuvine pueraria |
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| CN2011101328946A CN102250073A (en) | 2011-05-19 | 2011-05-19 | Method for directly extracting puerarin from fresh kudzuvine pueraria |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103006769A (en) * | 2013-01-05 | 2013-04-03 | 山东沃华医药科技股份有限公司 | Traditional Chinese medicine composition for treating cardiovascular and cerebrovascular diseases and preparation method thereof |
| CN114097967A (en) * | 2021-10-22 | 2022-03-01 | 湖南张家界九天生物科技有限责任公司 | Kudzu vine root beverage |
-
2011
- 2011-05-19 CN CN2011101328946A patent/CN102250073A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 孙术国 等: "高速剪切与微波辅助偶联提取鲜葛中葛根素", 《食品科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103006769A (en) * | 2013-01-05 | 2013-04-03 | 山东沃华医药科技股份有限公司 | Traditional Chinese medicine composition for treating cardiovascular and cerebrovascular diseases and preparation method thereof |
| CN114097967A (en) * | 2021-10-22 | 2022-03-01 | 湖南张家界九天生物科技有限责任公司 | Kudzu vine root beverage |
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Application publication date: 20111123 |