CN107455549A - A kind of method of albumen in surfactant auxiliary Enzymatic Extraction bean dregs - Google Patents
A kind of method of albumen in surfactant auxiliary Enzymatic Extraction bean dregs Download PDFInfo
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- CN107455549A CN107455549A CN201710909961.8A CN201710909961A CN107455549A CN 107455549 A CN107455549 A CN 107455549A CN 201710909961 A CN201710909961 A CN 201710909961A CN 107455549 A CN107455549 A CN 107455549A
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- bean dregs
- albumen
- surfactant
- enzymatic extraction
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23J1/148—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by treatment involving enzymes or microorganisms
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- Food Science & Technology (AREA)
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Abstract
The present invention relates to the method for albumen in food and food processing field, more particularly to a kind of surfactant auxiliary Enzymatic Extraction bean dregs.The inventive method is using the accessory substance bean dregs in soybean protein isolate production process as raw material, bean dregs material liquid pH is adjusted with alkali, cellulase is added to be hydrolyzed with surfactant, the addition of pH value of solution, hydrolysis temperature, hydrolysis time and enzyme and surfactant in comparative analysis bean dregs hydrolytic process, the protein content remained after being hydrolyzed eventually through measure in bean dregs obtain the optimum condition of albumen in surfactant auxiliary Enzymatic Extraction bean dregs.Experiment confirms, the inventive method is high to the recovery rate of albumen in bean dregs, residual protein amount is few, technique is simple, is easy to promote.
Description
Technical field
The present invention relates in food and food processing field, more particularly to a kind of surfactant auxiliary Enzymatic Extraction bean dregs
The method of albumen.
Background technology
Substantial amounts of accessory substance is produced in soybean protein isolate production process --- bean dregs, often process 1 ton of soybean and about produce 1.2
The wet bean dregs of ton, the wet bean dregs for being often only soybean protein isolate by-product just can reach thousands of tons of, however, these bean dregs do not obtain
Make full use of, mostly for feedstuff industry, greatly reduce its value, some is even directly discarded, not only wastes money
Source, while return environment and cause pollution, therefore, bean dregs resource is comprehensively utilized, its surcharge is lifted and prevents it
Environment is polluted, there is important economy and social effect.
Containing substantial amounts of cellulose, protein, vitamin, isoflavones, mineral matter in soybean bean dregs, there is higher nutrition
Value, and it is cheap, safe and healthy.Wherein, protein accounts for the 25% of soybean bean dregs total amount, institute containing human body in bean dregs albumen
All essential amino acids needed, similar with many vegetable proteins, it has relatively low allergenicity, is suitable for addition to infant food
In.
Because water soluble protein has been extracted substantially in soybean protein isolate production process, so being remained in bean dregs
Remaining protein is nearly all insoluble in water, and these slightly solubility albumen be mainly wrapped in inside cell membrane or with cell membrane or
Close membrane is connected, therefore the extraction to this partially protein has very big difficulty.
At present, the extraction process for albumen in soybean bean dregs mainly has at ferment treatment, high-temperature process, alkali process and ultrasound
Reason.Similar research is for example：Patent application " extracts dietary fiber and method of protein " from bean dregs
(CN201611047997.1) it is to smash wet bean dregs into okara powder, degreasing is extracted after drying, then adds alkaline reaction, is obtained
Supernatant and sediment, then acid liquid reaction will be added in supernatant, it is protein to obtain sediment；A kind of patent application " beans
One is disclosed in the extracting method of residue protein matter and its method for preparing DPP-IV peptide for inhibiting " (CN201410455009.1)
The extracting method of kind Protein from Soy Bean Dregs, it is that new fresh bean dreg and the mixed bean dregs suspension of water are positioned over 30-50 DEG C, when pH is
Taken out during 3.0-5.0, collect undermost Protein from Soy Bean Dregs after suspension layering, room temperature is dried；Patent of invention " is extracted from bean dregs
The method of soybean dietary fiber and soybean protein " (CN200910144467.2) system utilizes ultrasound-assisted enzymolysis collaboration food acid
Soybean dietary fiber and soybean protein are extracted in taste agent, this method by new fresh bean dreg it is dry, crush, okara powder of sieving to obtain, to bean dregs
The extract solution containing cellulase is added in powder and synchronizing ultrasound extracts to obtain extract solution；Supernatant and precipitation are obtained after extract solution centrifugation
Thing；Gained sediment dissolves to obtain supernatant and sediment through weak base；Supernatant precipitates through acid condiment, dries, crush after
Soybean protein.
The above method can extract the albumen in soybean bean dregs, however, still respectively have weak point, ferment treatment, high-temperature process
Protein extraction in bean dregs is had little effect；Although alkali process can effectively improve the recovery rate of albumen in bean dregs, alkaline
The too strong protein that can result in extremely is denatured, and produces noxious material --- rely alanine, in addition, base excess causes later stage acid dosage
Increase, produces more inorganic salts, makes content of ashes in product high, and certain influence is produced to product quality, safety；Ultrasonic wave
Method, although cell membrane can be destroyed, protein is discharged, ultrasonic device need to be introduced, increases production cost, and produced in extraction process
Raw lasting noise, pollutes environment.
It is auxiliary to establish a set of new utilization surfactant by the improvement to bean dregs protein extraction process conditions by the present invention
The method for helping albumen in Enzymatic Extraction bean dregs.The inventive method is simple to operate, production takes short, safety and environmental protection, extraction effect shows
Write, effective solution is provided for the comprehensive utilization of bean dregs resource.
The content of the invention
It is contemplated that overcoming, the recovery rate of existing soybean bean dregs protein extracting method is low, cumbersome, equipment requirement is high,
The deficiencies of polluting environment, there is provided a kind of simple to operate, production takes short, safety and environmental protection, the significant bean dregs albumen of extraction effect carries
Method is taken, effective solution is provided for the comprehensive utilization of bean dregs resource.
The method of albumen, comprises the following steps in surfactant auxiliary Enzymatic Extraction bean dregs of the present invention：
(1) ferment treatment：Raw material bean dregs are soaked in the water of 10 times of amounts, adjust pH to 8.0-8.5 with alkali, then add
0.5-1 ‰ cellulase and 50-100ppm surfactant, are uniformly mixing to obtain mixed liquor；
(2) hydrolyze：Container equipped with above-mentioned mixed liquor is placed in water-bath and stirs hydrolysis, mixing speed 150-
250r/min, bath temperature are 50-60 DEG C, hydrolysis time 0.5-1h；
(3) centrifuge：Feed liquid after hydrolysis is centrifuged, centrifugal speed 150-200r/min, during centrifugation
Between 5-10min；
(4) Solid separate for centrifuging to obtain to upper step carries out the measure of protein content.
Preferably, the purity ＞ 99% of cellulase described in the inventive method, enzyme activity 200-500U/g.
Preferably, surfactant described in the inventive method is that Tween-80 (is commonly called as：Tell
Temperature -80).
It is further preferred that raw material bean dregs are soaked in the water of 10 times of amounts in the inventive method step (1), adjusted with alkali
PH to 8.2, the addition of cellulase is 0.5 ‰, and the addition of Tween-80 is 100ppm.
It is further preferred that the hydrolysising condition in the inventive method step (2) is：Container equipped with mixed liquor is placed
Hydrolysis, mixing speed 200r/min are stirred in water-bath, bath temperature is 55 DEG C, hydrolysis time 0.5h.
In addition, raw material bean dregs described in the inventive method is the accessory substance in soybean protein isolate production process.
Preferably, alkali described in the inventive method is industrial lye.
It is further preferred that the NaOH solution that it is 30-32% that industrial lye described in the inventive method, which is concentration,.
The present invention is by under the conditions of appropriate weak base, adding cellulase into bean dregs suspension and surfactant being told
Temperature -80, water bath with thermostatic control heating extraction is then carried out, cell membrane and cell are effectively destroyed using the enzymolysis of cellulase
Film, the protein delivery being wrapped in cell membrane and cell membrane is come out, while surface active agent tween -80 can change solution
Surface tension, promote the dissolving of albumen, cooperated with the enzymolysis of cellulase and be remarkably improved carrying for albumen in bean dregs
Take rate, by determine digest before and after in bean dregs albumen content, available for judging its extraction effect.After tested, bean dregs of the present invention
Protein extracting method bean dregs albumen extraction rate is apparently higher than existing method, and the inventive method is simple to operate, low for equipment requirements,
It is especially suitable for enterprise's use of large-scale production.
Embodiment
Illustrate embodiments of the present invention below by way of specific instantiation, those skilled in the art can be by this specification
Disclosed content understands other advantages and effect of the present invention easily.The present invention can also pass through specific realities different in addition
The mode of applying is embodied or practiced, the various details in this specification can also be based on different viewpoints with application, without departing from
Various modifications or alterations are carried out under the spirit of the present invention.
Before the specific embodiment of the invention is further described, it should be appreciated that protection scope of the present invention is not limited to down
State specific specific embodiment；It is also understood that the term used in the embodiment of the present invention is specific specific in order to describe
Embodiment, the protection domain being not intended to be limiting of the invention.
When embodiment provides number range, it should be appreciated that except non-invention is otherwise noted, two ends of each number range
Any one numerical value can be selected between point and two end points.Unless otherwise defined, in the present invention all technologies for using and
Scientific terminology is identical with the meaning that those skilled in the art of the present technique are generally understood that.Except used in embodiment specific method, equipment,
Outside material, according to grasp of the those skilled in the art to prior art and the record of the present invention, it can also use and this
Any method, equipment and the material of the similar or equivalent prior art of method, equipment described in inventive embodiments, material come real
The existing present invention.
Embodiment 1
The method of albumen, comprises the following steps in a kind of surfactant auxiliary Enzymatic Extraction bean dregs：
(1) ferment treatment：By raw material bean dregs (its protein content is 33.6% after testing) immersion of soybean protein isolate by-product
In the water of 10 times of amounts, pH to 8.5 is adjusted with industrial lye NaOH solution, then adds 1 ‰ cellulase, enzyme purity ＞
99%, enzyme activity 200-500U/g, while 50ppm Tween-80 is added, it is uniformly mixing to obtain mixed liquor；
(2) hydrolyze：Container equipped with above-mentioned mixed liquor is placed in water-bath and stirs hydrolysis, mixing speed is
200r/min, bath temperature are 50 DEG C, hydrolysis time 0.5h；
(3) centrifuge：Feed liquid after hydrolysis is centrifuged, centrifugal speed 150r/min, centrifugation time
10min；
(4) Solid separate for centrifuging to obtain to upper step carries out the measure of protein content, and measuring its protein content is
10.1%.
Embodiment 2
The method of albumen, comprises the following steps in a kind of surfactant auxiliary Enzymatic Extraction bean dregs：
(1) ferment treatment：By raw material bean dregs (its protein content is 33.6% after testing) immersion of soybean protein isolate by-product
In the water of 10 times of amounts, pH to 8.0 is adjusted with industrial lye NaOH solution, then adds 0.5 ‰ cellulase, enzyme purity ＞
99%, enzyme activity 200-500U/g, while 75ppm Tween-80 is added, it is uniformly mixing to obtain mixed liquor；
(2) hydrolyze：Container equipped with above-mentioned mixed liquor is placed in water-bath and stirs hydrolysis, mixing speed is
200r/min, bath temperature are 60 DEG C, hydrolysis time 1h；
(3) centrifuge：Feed liquid after hydrolysis is centrifuged, centrifugal speed 200r/min, centrifugation time
5min；
(4) Solid separate for centrifuging to obtain to upper step carries out the measure of protein content, and measuring its protein content is
11.4%.
Embodiment 3
The method of albumen, comprises the following steps in a kind of surfactant auxiliary Enzymatic Extraction bean dregs：
(1) ferment treatment：By raw material bean dregs (its protein content is 33.6% after testing) immersion of soybean protein isolate by-product
In the water of 10 times of amounts, pH to 8.2 is adjusted with industrial lye NaOH solution, then adds 0.5 ‰ cellulase, enzyme purity ＞
99%, enzyme activity 200-500U/g, while 100ppm Tween-80 is added, it is uniformly mixing to obtain mixed liquor；
(2) hydrolyze：Container equipped with above-mentioned mixed liquor is placed in water-bath and stirs hydrolysis, mixing speed is
200r/min, bath temperature are 55 DEG C, hydrolysis time 0.5h；
(3) centrifuge：Feed liquid after hydrolysis is centrifuged, centrifugal speed 150r/min, centrifugation time
10min；
(4) Solid separate for centrifuging to obtain to upper step carries out the measure of protein content, and measuring its protein content is
9.0%.
Comparative example 1
Extracted according to the step in patent (publication number CN101627813A) embodiment 4, wet bean dregs protein content is
33.6%, sediment B is carried out to the measure of protein content, measures its protein content as 15.7%.
Comparative test result
Embodiment 1-3 and the result of implementation of comparative example 1 are as shown in table 1 below.
The embodiment 1-3 of table 1 and the result of implementation of comparative example 1
| Method | Protein content in bean dregs | Extraction rate |
| Embodiment 1 | 10.1% | 69.94% |
| Embodiment 2 | 11.4% | 66.07% |
| Embodiment 3 | 9.0% | 73.21% |
| Comparative example 1 | 15.7% | 53.27% |
As can be seen from Table 1, compared with comparative example 1, the inventive method (embodiment 1-3) significantly improves albumen in bean dregs
Extraction rate, highest can bring up to the extraction rate of albumen 73.21%, and Enzymatic Extraction mild condition, easy to operate, cost compared with
It is low, there is good development prospect.
The preferred embodiments of the disclosure and embodiment are explained in detail above, but the present invention is not limited to
The above-described embodiment and examples, in those skilled in the art's possessed knowledge, the present invention can also not departed from
Various changes can be made on the premise of design.
Claims (8)
1. a kind of method of albumen in surfactant auxiliary Enzymatic Extraction bean dregs, it is characterised in that comprise the following steps：
(1) ferment treatment：Raw material bean dregs are soaked in the water of 10 times of amounts, adjust pH to 8.0-8.5 with alkali, then add 0.5-
1 ‰ cellulase and 50-100ppm surfactant, are uniformly mixing to obtain mixed liquor；
(2) hydrolyze：Container equipped with above-mentioned mixed liquor is placed in water-bath and stirs hydrolysis, mixing speed 150-
250r/min, bath temperature are 50-60 DEG C, hydrolysis time 0.5-1h；
(3) centrifuge：Feed liquid after hydrolysis is centrifuged, centrifugal speed 150-200r/min, centrifugation time 5-
10min；
(4) Solid separate for centrifuging to obtain to upper step carries out the measure of protein content.
2. the method for albumen in surfactant auxiliary Enzymatic Extraction bean dregs as claimed in claim 1, wherein the cellulose
The purity ＞ 99% of enzyme, enzyme activity 200-500U/g.
3. the method for albumen in surfactant auxiliary Enzymatic Extraction bean dregs as claimed in claim 1, wherein the surface is lived
Property agent is Tween-80.
4. the method for albumen in surfactant as claimed in claim 3 auxiliary Enzymatic Extraction bean dregs, by original in its step (1)
Material bean dregs are soaked in the water of 10 times of amounts, adjust pH to 8.2 with alkali, the addition of cellulase is 0.5 ‰, polyoxyethylene
The addition of alcohol acid anhydride monoleate is 100ppm.
5. the method for albumen in surfactant as claimed in claim 4 auxiliary Enzymatic Extraction bean dregs, the water in its step (2)
Solution condition is：Container equipped with mixed liquor is placed in water-bath and stirs hydrolysis, mixing speed 200r/min, water-bath temperature
Spend for 55 DEG C, hydrolysis time 0.5h.
6. the method for albumen in surfactant auxiliary Enzymatic Extraction bean dregs as claimed in claim 1, wherein the material bean
Slag is the accessory substance in soybean protein isolate production process.
7. the method for albumen in surfactant auxiliary Enzymatic Extraction bean dregs as claimed in claim 1, wherein the alkali is work
Industry liquid caustic soda.
8. the method for albumen in surfactant auxiliary Enzymatic Extraction bean dregs as claimed in claim 7, wherein the industrial liquid
Alkali is the NaOH solution that concentration is 30-32%.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108929892A (en) * | 2018-08-02 | 2018-12-04 | 扬州市龙伟食品有限公司 | A method of extracting soluble bean dregs albumen and polypeptide from bean dregs |
| CN109170126A (en) * | 2018-10-16 | 2019-01-11 | 克东禹王大豆蛋白食品有限公司 | The extracting method of albumen in a kind of new bean dregs |
| CN109170919A (en) * | 2018-10-16 | 2019-01-11 | 克东禹王大豆蛋白食品有限公司 | A kind of method of surfactant auxiliary Enzymatic Extraction bean dregs |
| CN109251233A (en) * | 2018-09-21 | 2019-01-22 | 贵州省三好食品开发有限公司 | The method of legumin is extracted in a kind of bean dregs |
| CN109329555A (en) * | 2018-11-15 | 2019-02-15 | 中国林业科学研究院资源昆虫研究所 | A kind of separation preparation method of Moringa seed crude protein and albumen powder |
| WO2024123508A1 (en) * | 2022-12-08 | 2024-06-13 | Dow Global Technologies Llc | Oilseed cakes protein extraction composition |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108929892A (en) * | 2018-08-02 | 2018-12-04 | 扬州市龙伟食品有限公司 | A method of extracting soluble bean dregs albumen and polypeptide from bean dregs |
| CN108929892B (en) * | 2018-08-02 | 2021-12-10 | 扬州市龙伟食品有限公司 | Method for extracting soluble bean dregs protein and polypeptide from bean dregs |
| CN109251233A (en) * | 2018-09-21 | 2019-01-22 | 贵州省三好食品开发有限公司 | The method of legumin is extracted in a kind of bean dregs |
| CN109251233B (en) * | 2018-09-21 | 2021-11-12 | 贵州省三好食品开发有限公司 | Method for extracting bean protein from bean dregs |
| CN109170126A (en) * | 2018-10-16 | 2019-01-11 | 克东禹王大豆蛋白食品有限公司 | The extracting method of albumen in a kind of new bean dregs |
| CN109170919A (en) * | 2018-10-16 | 2019-01-11 | 克东禹王大豆蛋白食品有限公司 | A kind of method of surfactant auxiliary Enzymatic Extraction bean dregs |
| CN109170919B (en) * | 2018-10-16 | 2022-04-01 | 克东禹王大豆蛋白食品有限公司 | Method for extracting bean dregs by surfactant-assisted enzyme method |
| CN109170126B (en) * | 2018-10-16 | 2022-04-01 | 克东禹王大豆蛋白食品有限公司 | Novel method for extracting protein from bean dregs |
| CN109329555A (en) * | 2018-11-15 | 2019-02-15 | 中国林业科学研究院资源昆虫研究所 | A kind of separation preparation method of Moringa seed crude protein and albumen powder |
| WO2024123508A1 (en) * | 2022-12-08 | 2024-06-13 | Dow Global Technologies Llc | Oilseed cakes protein extraction composition |
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Application publication date: 20171212 |