CN109329555A - A kind of separation preparation method of Moringa seed crude protein and albumen powder - Google Patents
A kind of separation preparation method of Moringa seed crude protein and albumen powder Download PDFInfo
- Publication number
- CN109329555A CN109329555A CN201811357010.5A CN201811357010A CN109329555A CN 109329555 A CN109329555 A CN 109329555A CN 201811357010 A CN201811357010 A CN 201811357010A CN 109329555 A CN109329555 A CN 109329555A
- Authority
- CN
- China
- Prior art keywords
- moringa
- powder
- crude protein
- oil
- seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23J1/142—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by extracting with organic solvents
- A23J1/144—Desolventization
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a kind of Moringa seed crude protein and the separation preparation methods of albumen powder, comprising the following steps: step 1: picking selenium-rich Moringa maturation beanpod takes out seed, air-dries or dry, shelled with Moringa hulling machine, obtain Moringa kernel；Step 2: Moringa kernel is handled using squeezing method or solvent extraction method, oil ben is removed, obtains Moringa seed crude protein, protein content reaches 53-56% in crude protein；Step 3: in the deionized water that pH value is 3-11, emulsifier is added, and heat spare；Step 4: deionized water is added in Moringa crude protein, ultrasonic extraction separation, filtering, filtrate is with spray drying process drying to get the selenium-rich Moringa seed separated protein powder arrived.The method that emulsifier is added in the present invention during the extraction process increases the dissolution rate of fat-soluble albumen in Moringa seed, improve the yield of protein isolate, the product blank of Moringa protein isolate has been filled up in implementation of the invention, increases the available sources of high-end commodity egg white powder.
Description
Technical field
The present invention relates to albumen preparation technique fields, more particularly to the separation of a kind of Moringa seed crude protein and albumen powder
Preparation method.
Background technique
Moringa nutriment type is more, rich in the mineral nutrient elements such as vitamin A, B, C, D, E and calcium, potassium, iron, additionally
Containing various amino acid needed by human and microelement etc., nutritive value and modern nutriology man are known as " human nutrition
Miniature treasure-house ' spirulina it is suitable, its protein and vitamin abundant, amino acid are not only the reason of developed country vegetarian
Think food, or the natural nutrition library of poor regional women and children.
Average oil concentration 35% in Moringa kernel, crude protein content 35.0-43.0%'s, than peanut crude protein content
(33.1%) high.Amino acid content in Moringa is high, and type is more, it is necessary to which total amino acid content is high, leaf Glutamic Acid (Glu), asparagus fern
Amide (Asp), lysine (Lys), leucine (Leu), arginine (Arg) rich content, arginine (Arg) content is outstanding in seed
It is high, higher than content in peanut by 22.48%, higher than soy content by 40.73%, and peanut be typically considered arginine content compared with
The content of the high and biggish nut of biological effect, methionine (Met) and cysteine (Cys) in Moringa seed is also significantly high
In peanut and soybean, also, Moringa is higher than peanut and soybean in the ratio that essential amino acids content accounts for total amino acid content.It is peppery
First limiting amino acid of the wooden seed is lysine (Lys), therefore, Moringa leaf protein is added in Moringa seed albumen powder, can
To improve the nutritive value of Seed Storage Protein powder.But it is right at present to the research of vegetalitas table egg bletilla amino acid based on soybean
The research and exploitation of Moringa albumen are less.
Summary of the invention
The object of the invention is that providing a kind of Moringa seed crude protein and albumen powder to solve the above-mentioned problems
Separate preparation method.
The present invention through the following technical solutions to achieve the above objectives:
A kind of separation preparation method of Moringa seed crude protein and albumen powder, comprising the following steps:
Step 1: picking selenium-rich Moringa maturation beanpod takes out seed, air-dries or dry, with Moringa hulling machine to Moringa kind
Son shelling, obtains Moringa kernel；
Step 2: Moringa kernel is handled using squeezing method or solvent extraction method, removes oil ben；
Squeezing method oil removing: by oil press preheating temperature to 80-110 DEG C, not heated Moringa kernel is directly extracted oil, and is obtained
Oil ben and Moringa oil cake crush Moringa oil cake to 100-200 mesh, obtain Moringa seed crude protein powder；
Solvent extraction method oil removing: shelling Moringa kernel is first crushed to 100-200 mesh, according to quality than moringa powder: solvent
For 1:10-1:15, petroleum ether (60-90 DEG C) is added in kind of powder, extracts 8-10h, filtering, filter cake compares Moringa again according to quality
Powder: solvent is that petroleum ether (60-90 DEG C) is added in 1:10-1:15, extracts 8-10h, filtering, and merging filtrate concentration and recovery solvent obtains
To oil ben, filter cake is crushed to 100-200 mesh, as Moringa seed crude protein powder after air-drying, and detects Moringa with Kjeldahl's method
The protein content of seed crude protein powder is 53-56%；
Step 3: removing ionized water, and the pH value for adjusting deionized water is 3-11, is added in the deionized water for mixing up pH value
Emulsifier, additional amount is the 2-5% of mass fraction, and water is heated to 30-60 DEG C, spare；
Step 4: in Moringa seed crude protein powder, being added ready deionized water, according to quality than crude protein powder:
Deionized water is 1:10-150, ultrasonic extraction 20-40min, is filtered, and filtrate is peppery to get the selenium-rich arrived with spray drying process drying
The wooden seed separated protein powder is 80-90% through Kjeldahl's method detection protein content, and total Se content is former in separated protein powder
Sub- fluorescence measurement is set to 10-40 μ g/g, albumen Se content 8-30 μ g/g.
Preferably, Moringa hulling machine feed rate is 5-7kg/min in the step 1.
Preferably, emulsifier is Tween-60, Tween-80, Arlacel-80, Arlacel-85, lecithin in the step 3
One or more of.
Preferably, spray drying condition in the step 4 are as follows: 150-170 DEG C of inlet air temperature, enter the wind flow 0.6-
0.8m3/ min, feed rate 300-400mL/h, atomizing pressure 180-250kPa.
Compared with prior art, advantages of the present invention is as follows:
1, the method that emulsifier is added during the extraction process increases the dissolution rate of fat-soluble albumen in Moringa seed, improves
The yield of protein isolate；
2, the product blank of Moringa protein isolate has been filled up in implementation of the invention, increases the source of high-end commodity egg white powder
Approach.
Specific embodiment
The present invention is further illustrated combined with specific embodiments below, but the invention is not limited to these to implement
Example.
Embodiment 1
A kind of separation preparation method of Moringa seed crude protein and albumen powder, comprising the following steps:
Step 1: picking selenium-rich Moringa maturation beanpod takes out seed, air-dries or dry, with Moringa hulling machine to Moringa kind
Son shelling, feed rate 5kg/min obtain Moringa kernel；
Step 2: handling Moringa kernel using squeezing method, remove oil ben, by oil press preheating temperature to 90 DEG C, without
The Moringa kernel of heating is directly extracted oil, oil yield 29%, obtains oil ben and Moringa oil cake, is crushed Moringa oil cake to 100 mesh, is obtained
To Moringa seed crude protein powder, the protein content with Kjeldahl's method detection Moringa seed crude protein powder is 53%；
Step 3: removing ionized water, is 3 with the pH value that 0.1mol/L hydrochloric acid adjusts deionized water, according to mass fraction 2%
Emulsifier tween -80 is added in water, and water is heated to 40 DEG C, it is spare；
Step 4: taking 1kg in Moringa seed crude protein powder, and ready deionized water 10kg, ultrasonic extraction is added
20min, filtering, filtrate is dry with spray drying process, spray drying condition are as follows: 150 DEG C of inlet air temperature, enters the wind flow 0.6m3/
Min, feed rate 300mL/h, atomizing pressure 180kPa are to get the selenium-rich Moringa seed separated protein powder arrived, through kjeldahl determination
It is 85% that method, which detects protein content, and total Se content Atomic Fluorescence Spectrometry is 22 μ g/g in separated protein powder, and albumen selenium contains
Measure 16 μ g/g.
Embodiment 2
A kind of separation preparation method of Moringa seed crude protein and albumen powder, comprising the following steps:
Step 1: picking selenium-rich Moringa maturation beanpod takes out seed, air-dries or dry, with Moringa hulling machine to Moringa kind
Son shelling, feed rate 6kg/min obtain Moringa kernel；
Step 2: handling Moringa kernel using squeezing method, remove oil ben, by oil press preheating temperature to 100 DEG C, without
The Moringa kernel of heating is directly extracted oil, oil yield 34%, obtains oil ben and Moringa oil cake, is crushed Moringa oil cake to 200 mesh, is obtained
To Moringa seed crude protein powder；
Step 3: removing ionized water, and emulsifier span -80 is added according to mass fraction 3% in pH value 7 in water, and
Water is heated to 50 DEG C, it is spare；
Step 4: taking Moringa seed crude protein powder 0.2kg, and ready deionized water 4kg is added, according to the thick egg of mass ratio
White powder: deionized water 1:20, ultrasonic extraction 30min, filtering, filtrate is dry with spray drying process, spray drying condition are as follows: into
160 DEG C of air temperature, enter the wind flow 0.7m3/ min, feed rate 350mL/h, atomizing pressure 200kPa are to get the selenium-rich Moringa arrived
Seed separated protein powder is 87% through Kjeldahl's method detection protein content, and total Se content atom is glimmering in separated protein powder
Light method is measured as 30 μ g/g, 20 μ g/g of albumen Se content.
Embodiment 3
A kind of separation preparation method of Moringa seed crude protein and albumen powder, comprising the following steps:
Step 1: picking selenium-rich Moringa maturation beanpod takes out seed, air-dries or dry, with Moringa hulling machine to Moringa kind
Son shelling, feed rate 7kg/min obtain Moringa kernel；
Step 2: handling Moringa kernel using solvent extraction method, removes oil ben, first shelling Moringa kernel is crushed to
200 mesh, according to quality than moringa powder: petroleum ether (60-90 DEG C) is added in kind of powder in solvent 1:10, extracts 8h, filters, filter
Cake is again according to quality than moringa powder: solvent is that petroleum ether (60-90 DEG C) is added in 1:10, extracts 10h, filters, merging filtrate is dense
Contracting recycling design, obtains oil ben, and filter cake is crushed to 200 mesh, as Moringa seed crude protein powder after air-drying, uses Kjeldahl's method
The protein content for detecting Moringa seed crude protein powder is 56%；
Step 3: removing ionized water, and adjusting pH value with 0.1mol/LNaOH is 9, is added in water according to mass fraction 5%
Emulsifier tween -60, and water is heated to 60 DEG C, it is spare；
Step 4: taking Moringa seed crude protein powder 0.1kg, and ready deionized water 10kg is added, thick according to mass ratio
Albumen powder: deionized water 1:100, ultrasonic extraction 40min, filtering, filtrate is dry with spray drying process, spray drying condition
Are as follows: 170 DEG C of inlet air temperature, enter the wind flow 0.8m3/ min, feed rate 400mL/h, atomizing pressure 250kPa is to get the richness arrived
Selenium Moringa seed separated protein powder is 89% through Kjeldahl's method detection protein content, and total Se content is used in separated protein powder
Atomic Fluorescence Spectrometry is 40 μ g/g, 30 μ g/g of albumen Se content.
Embodiment described above only expresses embodiments of the present invention, and the description thereof is more specific and detailed, but can not be because
This and be interpreted as the limitation of the invention patent range.It should be pointed out that for those of ordinary skill in the art, not
Under the premise of being detached from present inventive concept, various modifications and improvements can be made, and these are all within the scope of protection of the present invention.
Claims (4)
1. the separation preparation method of a kind of Moringa seed crude protein and albumen powder, it is characterised in that: the following steps are included:
Step 1: picking selenium-rich Moringa maturation beanpod takes out seed, air-dries or dry, de- to Moringa seed with Moringa hulling machine
Shell obtains Moringa kernel；
Step 2: Moringa kernel is handled using squeezing method or solvent extraction method, removes oil ben；
Squeezing method oil removing: by oil press preheating temperature to 80-110 DEG C, not heated Moringa kernel is directly extracted oil, and obtains Moringa
Oil and Moringa oil cake crush Moringa oil cake to 100-200 mesh, obtain Moringa seed crude protein powder；
Shelling Moringa kernel: being first crushed to 100-200 mesh by solvent extraction method oil removing, according to quality than moringa powder: solvent 1:
Petroleum ether (60-90 DEG C) is added in kind of powder in 10-1:15, extracts 8-10h, filtering, filter cake is again according to quality than moringa powder:
Solvent is that petroleum ether (60-90 DEG C) is added in 1:10-1:15, extracts 8-10h, filtering, and merging filtrate concentration and recovery solvent obtains peppery
Wood oil, filter cake are crushed to 100-200 mesh, as Moringa seed crude protein powder after air-drying；
Step 3: removing ionized water, and the pH value for adjusting deionized water is 3-11, and emulsification is added in the deionized water for mixing up pH value
Agent, additional amount is the 2-5% of mass fraction, and water is heated to 30-60 DEG C, spare；
Step 4: in Moringa seed crude protein powder, being added ready deionized water, according to quality than crude protein powder: go from
Sub- water is 1:10-150, ultrasonic extraction 20-40min, and filtering, filtrate is with spray drying process drying to get the selenium-rich Moringa kind arrived
Sub- separated protein powder.
2. the separation preparation method of a kind of Moringa seed crude protein according to claim 1 and albumen powder, it is characterised in that:
Moringa hulling machine feed rate is 5-7kg/min in the step 1.
3. the separation preparation method of a kind of Moringa seed crude protein according to claim 1 and albumen powder, it is characterised in that:
Emulsifier is one or more of Tween-60, Tween-80, Arlacel-80, Arlacel-85, lecithin in the step 3.
4. the separation preparation method of a kind of Moringa seed crude protein according to claim 1 and albumen powder, it is characterised in that:
Spray drying condition in the step 4 are as follows: 150-170 DEG C of inlet air temperature, enter the wind flow 0.6-0.8m3/ min, feed rate
300-400mL/h, atomizing pressure 180-250kPa.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811357010.5A CN109329555A (en) | 2018-11-15 | 2018-11-15 | A kind of separation preparation method of Moringa seed crude protein and albumen powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811357010.5A CN109329555A (en) | 2018-11-15 | 2018-11-15 | A kind of separation preparation method of Moringa seed crude protein and albumen powder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109329555A true CN109329555A (en) | 2019-02-15 |
Family
ID=65315313
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811357010.5A Pending CN109329555A (en) | 2018-11-15 | 2018-11-15 | A kind of separation preparation method of Moringa seed crude protein and albumen powder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109329555A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110432333A (en) * | 2019-08-30 | 2019-11-12 | 黑龙江省上草堂野生荭菇娘研究开发有限公司 | A kind of groundcherry Calyx Physalis seed hypoglycemic polysaccharide protein reconstitutes the preparation method of product |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101508729A (en) * | 2009-03-19 | 2009-08-19 | 中国林业科学研究院资源昆虫研究所 | Method for extracting horseradish tree protein from horseradish tree seed |
| CN106282282A (en) * | 2016-08-10 | 2017-01-04 | 华南农业大学 | A kind of extract oils and fats and albumen and/or the method for glucosides in moringa seeds simultaneously |
| CN107455549A (en) * | 2017-09-29 | 2017-12-12 | 山东禹王生态食业有限公司 | A kind of method of albumen in surfactant auxiliary Enzymatic Extraction bean dregs |
-
2018
- 2018-11-15 CN CN201811357010.5A patent/CN109329555A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101508729A (en) * | 2009-03-19 | 2009-08-19 | 中国林业科学研究院资源昆虫研究所 | Method for extracting horseradish tree protein from horseradish tree seed |
| CN106282282A (en) * | 2016-08-10 | 2017-01-04 | 华南农业大学 | A kind of extract oils and fats and albumen and/or the method for glucosides in moringa seeds simultaneously |
| CN107455549A (en) * | 2017-09-29 | 2017-12-12 | 山东禹王生态食业有限公司 | A kind of method of albumen in surfactant auxiliary Enzymatic Extraction bean dregs |
Non-Patent Citations (3)
| Title |
|---|
| 杨倩等: "响应面优化提取小球藻蛋白质及其性质研究", 《中国食品学报》 * |
| 王兴国: "《油料科学原理》", 31 August 2017, 第2版 * |
| 程牛亮: "《生物化学》", 31 January 2007, 中国医药科技出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110432333A (en) * | 2019-08-30 | 2019-11-12 | 黑龙江省上草堂野生荭菇娘研究开发有限公司 | A kind of groundcherry Calyx Physalis seed hypoglycemic polysaccharide protein reconstitutes the preparation method of product |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104130333B (en) | Utilize purple potato synchronous production anthocyanidin, starch, cellulose and method of protein | |
| CN105994930A (en) | Process for processing high-purity fructus cannabis protein power | |
| RU2006101544A (en) | PRODUCTION OF FLOUR FROM OIL SEEDS | |
| CN105175382B (en) | A kind of comprehensive processing and utilization method of purple potato | |
| CN108558971A (en) | A kind of preparation method of roselle anthocyanin | |
| CN103666752A (en) | Red pine kernel oil extraction and purification technology | |
| CN103756782A (en) | Vegetable oil preparation method | |
| CN106833902A (en) | A kind of Japan pepper essential oil and its extracting method | |
| CN107361196B (en) | Process for simultaneously producing soybean protein isolate and soybean protein concentrate | |
| CN109329555A (en) | A kind of separation preparation method of Moringa seed crude protein and albumen powder | |
| CN104926719B (en) | A kind of method that trigonelline is extracted from fructus cannabis | |
| CN103923050A (en) | Method for preparing quercetin with rutin | |
| CN1329362C (en) | Method for extracting chlorogenic acid and sunflower protein from sunflower seed cake | |
| CN105294633B (en) | A kind of industrial method preparing cowberry anthocyanidin from cowberry | |
| CN104621339A (en) | Method for extracting silybum marianum protein isolate | |
| CN1220094A (en) | Linseed comprehensive utilization method | |
| CN103045354A (en) | Method for extracting seabuckthorn fruit by supercritical CO2 | |
| CN1631197A (en) | Technology for preparing concentrated soyabean protein by continuous gradient extraction | |
| CN103710137B (en) | Cashew nut oil and preparation method thereof | |
| CN102295987B (en) | Process for extracting laver oil from laver with solvent method | |
| CN104473059A (en) | Preparation method of purple perilla vegetarian meat | |
| CN101220078A (en) | Method for extracting protein from waste liquor from potato starch process | |
| CN104447664A (en) | Method for extracting anthocyanin from red rice pigment precipitation residues | |
| CN107011411A (en) | A kind of cottonseed protein production method that can extract a variety of byproducts | |
| WO2017185281A1 (en) | Fritillary flower extractive preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190215 |