CN108085356A - Using cold pressing walnut dregs as the method for primary industry metaplasia production of high purity walnut peptide - Google Patents
Using cold pressing walnut dregs as the method for primary industry metaplasia production of high purity walnut peptide Download PDFInfo
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- CN108085356A CN108085356A CN201711444692.9A CN201711444692A CN108085356A CN 108085356 A CN108085356 A CN 108085356A CN 201711444692 A CN201711444692 A CN 201711444692A CN 108085356 A CN108085356 A CN 108085356A
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- walnut
- protein
- peptide
- dregs
- enzymolysis
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- 235000009496 Juglans regia Nutrition 0.000 title claims abstract description 327
- 235000020234 walnut Nutrition 0.000 title claims abstract description 327
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 129
- 238000000034 method Methods 0.000 title claims abstract description 39
- 238000003825 pressing Methods 0.000 title claims abstract description 31
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 20
- 206010054949 Metaplasia Diseases 0.000 title claims abstract description 14
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- 102000004190 Enzymes Human genes 0.000 claims abstract description 84
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- 229940088598 enzyme Drugs 0.000 claims abstract description 84
- 238000005119 centrifugation Methods 0.000 claims abstract description 73
- 230000009849 deactivation Effects 0.000 claims abstract description 59
- 108010038851 tannase Proteins 0.000 claims abstract description 40
- 239000004382 Amylase Substances 0.000 claims abstract description 39
- 102000013142 Amylases Human genes 0.000 claims abstract description 39
- 108010065511 Amylases Proteins 0.000 claims abstract description 39
- 239000004365 Protease Substances 0.000 claims abstract description 39
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims abstract description 39
- 235000019418 amylase Nutrition 0.000 claims abstract description 39
- 108091005804 Peptidases Proteins 0.000 claims abstract description 33
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- 229940106157 cellulase Drugs 0.000 claims abstract description 30
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- 238000001914 filtration Methods 0.000 claims abstract description 19
- 238000011161 development Methods 0.000 claims abstract description 18
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 15
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 8
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- 239000004475 Arginine Substances 0.000 description 3
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- 108010068370 Glutens Proteins 0.000 description 2
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- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 2
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- 235000019606 astringent taste Nutrition 0.000 description 1
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 description 1
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- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
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- 238000000746 purification Methods 0.000 description 1
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 235000019158 vitamin B6 Nutrition 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- 235000020261 walnut milk Nutrition 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of using cold pressing walnut dregs as the method for primary industry metaplasia production of high purity walnut peptide.The present invention is dispersed through, using cold press walnut dregs as raw material after aquation, by tannase, cellulase, medium temperature amylase enzymolysis, is then denatured, then using alkali protease, neutral proteinase stepwise discretization, enzyme deactivation, centrifugation, plate-frame filtering obtain high-purity walnut peptide.The present invention is by pre-processing, digesting, the walnut peptide of the preparation of industrialization high-purity of the very economical property of the science of process for refining preferred implementation, protein content more than 90%, peptide content more than 85%, relative molecular weight is less than 2000Dal peptides accounting more than 85%, relative molecular weight is less than 1000Dal peptides accounting more than 50%, superior product quality, bitter taste is weak, is easy to arrange in pairs or groups with other food ingredients, brain tonic and intelligence development, strengthen immunity effect protrude, and can be widely used in as high-quality functional material in varieties of food items, health products.
Description
Technical field
The invention belongs to agricultural and sideline product high added value process and utilization technology fields, and in particular to one kind is with cold pressing walnut
The dregs of rice are the method for primary industry metaplasia production of high purity walnut peptide.
Background technology
Walnut is one of four big dry fruit of the world, because its abundant nutritive value is liked be subject to domestic and international consumer.Every 100
Fatty 63~70% (predominantly unrighted acids, account for the 90% of its total amount) in gram walnut kernel, protein 15~29%,
Carbohydrate 15%；In addition, also containing calcium, phosphorus, iron, zinc, magnesium, carrotene, riboflavin and vitamin B6, vitamin E, juglandis folia quinone,
The nutriments such as phosphatide.China is one of original producton location of walnut, there is the cultivation history of more than 2000 years, at present China's walnut cultivation face
Product and yield occupy first place in the world.However the degree and depth of walnut product utilization are far from enough, except traditional stir-fry eats, makes
Can, do mooncake filling, walnut powder, Walnut Milk, outside walnut oil, the deep processed product of other purposes is rarely seen.Walnut oil is listed in closely
One of 3 higher big vegetable oil of several years worlds, domestic market demand amounts, consumption proportion persistently rises.Domestic walnut oil factory
Family has reached tens of families, and substantial amounts of degreasing walnut grouts seriously waste petal resource by as feed, fertilizer or discarding.It grinds
Study carefully protein content in display walnut dregs and be up to more than 50%, walnut protein is mainly made of 4 proteinoid, i.e. albumin, ball
Albumen, alcohol soluble protein and glutelin account for walnut protein total amount 6.81%, 17.57%, 5.33% and 70.11%, albumen respectively
The standard that matter composition is recommended close to FAO (Food and Agriculture Organization of the United Nation) (FAO) and the World Health Organization (WHO), 8 kinds must amino acid content
Rationally, there are the glutamic acid, aspartic acid and arginine content of critical function higher Human Physiology effect, being that one kind is great opens
Send out the vegetable protein of potentiality.
Hydrophobic amino acid ratio is higher in walnut dregs glutelin, globulin, causes walnut protein dissolubility poor, significantly
Ground limits its utilization in the food industry, therefore there is important social economy to anticipate for the deep processing for studying walnut cake protein
Justice.In recent years, the study found that edible protein raw material can be obtained by scientific and reasonable biological engineering means with a variety of physiology work(
The active peptide of energy including absorption easy to digest, anti-oxidant, dispelling fatigue, hypoglycemic, blood pressure lowering, promotes alcohol metabolism, enhancing immune
The physiologically active peptides such as power belong to natural green functional food ingredient, and effect is notable, without side-effects, safe, can widely answer
For food (especially health food), medicine, there is good market application foreground, exploitation walnut peptide will be that walnut dregs are profound
Most potential and most promising direction in processing.
The effects that natural walnut protein molecular is due to hydrogen bond, hydrophobic bond, disulfide bond, peptide chain are curled near inside protein molecular
It is similar to spherical, close structure and has stronger hydrophobicity, it is difficult to by protease hydrolytic；In addition, also there are a great deal oves in walnut dregs
Starch, grease and pigment etc., affect the deep processing and utilization of walnut protein.At present, domestic and international walnut protein deep processing
Aspect research is mainly concentrated in efficiently digesting walnut protein, obtains the small-molecular-weight walnut peptide of high yield pulp1.It is prepared by walnut peptide
Prominent problem mainly includes：(1) product purity is low, i.e. walnut peptide protein content is relatively low, mainly still has one in walnut dregs
Quantitative starch, grease, fail to pre-process before enzymolysis, digest after effectively remove in separation and purification, the non-egg of final peptide product
White component content is high, influences the quality of product；(2) walnut protein compact structure, enzymolysis efficiency is low, low molecular weight in enzymolysis product
Peptide content is not high；(3) black containing walnut kernel skin, color and luster in walnut dregs and have bitter taste, processing not yet in effect can influence product
Color and luster and flavor；(4) preparation process it is cumbersome, without scientific optimization, it is of high cost, it is difficult to industrializing implementation.Such as patent
CN103109971B is disclosed using walnut dregs after walnut cold press as raw material, by defibrination, compound protease enzymolysis, membrane filtration, dense
Contracting is dried to obtain a kind of high walnut peptide of arginine content, and this method activity peptide yield is less than 30%, and product protein content is low,
Main peptide molecular weight≤3000kD；Patent CN 102630804A disclose one kind using the degreasing walnut dregs of rice as raw material, by the way that water is added to mix
Close uniformly, homogeneous, complex enzyme zymohydrolysis, enzyme deactivation, centrifugation, concentrate drying obtain a kind of walnut peptide powder, the method product protein content
Low, peptide molecular weight is in extensive range, small-molecular peptides content is low；Patent CN 103103244B disclose a kind of walnut blood pressure lowering peptide system
Using walnut dregs as raw material, walnut protein is purified using alkali extraction-acid precipitation for Preparation Method, then using microwave combination ultrasonication walnut
Moderately denaturation to improve enzymolysis efficiency, passes through 0.45um microfiltration membranes, 8kD ultrafiltration membranes, 1kD to albumen after adding in neutral protease enzymolysis
Ultrafiltration membrane collects permeate, B- cyclodextrin embeddings is added in permeate, vacuum freeze drying obtains walnut blood pressure lowering peptide, the method
Walnut dregs are handled using traditional alkali extraction-acid precipitation, because of the special compactness of walnut protein structure, it is difficult to obtain desired purity (egg
Bai Hanliang more than 90%) walnut protein, before another enzymolysis after denaturation treatment and enzymolysis UF membrane it is excessively complicated, to equipment requirement
Height is not easy to industrialize, and the production cost of product is high.
The content of the invention
In order to solve the difficult point of the above-mentioned prior art and shortcoming, primary and foremost purpose of the invention is to provide one kind with low
Temperature and pressure squeeze the method that walnut dregs are primary industry metaplasia production of high purity walnut peptide.This method using cold pressing walnut dregs as raw material,
Using tannase, cellulase, medium temperature starch enzymatic treatment, with reference to appropriateness heat treatment inactive enzyme, change protein structure and washing
Walnut protein is purified, adds compound protease stepwise discretization, enzyme deactivation, enzymolysis product is centrifuged, after plate compression, filtration
Liquid concentration, the dry walnut peptide for preparing high-purity, process intensification optimization are suitble to industrialized production.
Another object of the present invention is to provide the application of the method.
Another mesh of the present invention is to provide the high-purity walnut peptide being prepared by the above method.The peptide, which can use, goes bail for
The raw material of health food, ordinary food is used especially for brain tonic and intelligence development, strengthen immunity product.
The present invention is realized by following technical proposals：One kind is produced by primary industry metaplasia of cold pressing walnut dregs
The method of high-purity walnut peptide, comprises the following steps：
(1) the scattered of walnut dregs, aquation：Cold press walnut dregs are weighed, walnut protein solution is formulated as with water；By walnut protein
Solution shear is uniformly dispersed, aquation；
(2) tannase, cellulase, medium temperature amylase enzymolysis：The good walnut protein solution of scattered, aquation is heated to 45
DEG C~55 DEG C, add in tannase, cellulase and medium temperature amylase, enzymolysis；The quality dosage of each enzyme using walnut dregs quality as
Benchmark, it is as follows：Tannase 0.1%~0.2%, cellulase 0.1%~1.0%, medium temperature amylase 0.5~2.0%；
(3) protein denaturation, centrifugation, washing：Walnut protein solution after step (2) is digested is at 100~121 DEG C
0.5~1.0h is managed, centrifugation discards supernatant liquid (oil layer, aqueous layer), collects precipitation, and it is 50~60 DEG C to add temperature
Water stirs evenly, and centrifugation discards supernatant liquid, collects precipitation；
(4) protease stepwise discretization, enzyme deactivation：The water that temperature is 50~60 DEG C is added in the precipitation obtained in step (3), is stirred
It mixes uniformly, obtains walnut protein slurry；The pH value of walnut protein slurry is adjusted as 7.5~8.5, first add in alkali protease in 50 DEG C~
55 DEG C of stirring hydrolysis 1h~2h add neutral proteinase in 50 DEG C~55 DEG C stirring hydrolysis 4h~16h；It goes out after enzymolysis
Enzyme；The quality dosage of each enzyme by walnut protein starch in calculate on the basis of protein quality, it is as follows：Alkali protease 0.5%~
2%th, neutral proteinase 0.5%~2%；
(5) centrifuge, plate compression：Walnut protein enzymolysis product after enzyme deactivation is centrifuged, obtained clear liquid warp
Plate compression takes clarification filtered solution, obtains high-purity walnut peptide.
It is described using cold pressing walnut dregs as the method for primary industry metaplasia production of high purity walnut peptide, further include following step
Suddenly：
(6) concentrate, is dry, packaging：The clarification filtered solution that step (5) is obtained concentrates, and concentrate is spray-dried,
Obtain walnut peptide finished product.
Heretofore described water is preferably deionized water.
In step (1)：
The cold press walnut dregs refer to using the walnut dregs after hydraulic cold method degreasing.
The concentration of walnut dregs is preferably mass percent 5%~15% in the walnut protein solution.
The condition of the shearing is preferably 10~30min of speed shearing in 2000rpm~3000rpm；More preferably in
The speed of 2000rpm~3000rpm shears 10~20min.
In step (2)：
The temperature of the enzymolysis is preferably 45 DEG C~50 DEG C.
The time of the enzymolysis is preferably 30min~90min.
The enzymolysis preferably carries out under stirring, and mixing speed is preferably less than 60rpm, more preferably 18~
36rpm。
In step (3)：
The condition of the centrifugation is preferably to centrifuge 10min~20min in 3000rpm~6000rpm；More preferably in
3000rpm~4000rpm centrifuges 15min~20min.
The dosage of the water is preferably equivalent to the precipitation quality can be advisable the precipitation is fully dispersed
3~5 times.
The temperature of the water is preferably 50~55 DEG C.
In step (4)：
The dosage of the water is preferably equivalent to the precipitation quality can be advisable the precipitation is fully dispersed
3~8 times；More preferably 6~8 times.
The temperature of the water is preferably 55 DEG C.
The pH value is preferably 8~8.5.
The pH value is preferably adjusted by sodium hydroxide solution or hydrochloric acid solution.
The concentration of the sodium hydroxide solution is preferably 1mol.L-1~5mol.L-1。
The concentration of the hydrochloric acid solution is preferably 1mol.L-1~4mol.L-1。
The alkali protease is preferably at least one of pancreatin, trypsase and bacillus licheniformis protease.
The neutral proteinase is preferably Novi letter neutral proteinase Neutrase, papain, bromelain
At least one of with ficin.
The enzymolysis preferably carries out under stirring, and mixing speed is preferably less than 60rpm, more preferably 18~
36rpm。
The condition of the enzyme deactivation is preferably in 85~95 DEG C of 10~40min of enzyme deactivation；More preferably in 85~90 DEG C of enzyme deactivations 15
~30min.
In step (5)：
The centrifugation refers to that the walnut protein enzymolysis product after enzyme deactivation is pumped into decanter centrifuge centrifuges, and waste takes
Clear liquid.
The plate compression refers to, using plate-frame type diatomaceous earth filter press filtration, further remove remaining in centrifugal clear liquid
Protein and macromolecular peptide, be enriched with small-molecular peptides ingredient.
In step (6)：
It is 20~45% that the degree of the concentration, which is preferably concentrated into solid content,.
The concentration is preferably concentrated by economic benefits and social benefits or multiple-effect falling film evaporator.
The drying is preferably dried by drying tower, and operating parameter is preferably that inlet air temperature is 170 DEG C~200 DEG C, is gone out
Air temperature is 75 DEG C~95 DEG C.
Application of the method in industrialized production high-purity walnut peptide.
A kind of high-purity walnut peptide, is prepared by the above method.
The high-purity walnut peptide, walnut peptide protein content more than 90%, peptide content more than 85%, average molecular
Amount is less than 2000Dal peptides accounting more than 85%, and relative molecular weight is less than 1000Dal peptides accounting more than 50%.
The high-purity walnut peptide superior product quality, bitter taste is weak, is widely used as the original of health food, ordinary food
Material, is used especially for preparing brain tonic and intelligence development, strengthen immunity product.
Compared with prior art, the present invention having the following advantages that and advantageous effect：
(1) due to a certain amount of walnut shell of remaining in high temperature hot moulding method walnut dregs, it is not easy to remove, and protein has occurred sternly
Weight excessively denaturation, color and luster is deep, and the present invention use low-temperature cold pressing method by-product walnut dregs as raw material, cold pressing method process warm with
Protein denaturation degree is low, and substrate guarantee is provided for the walnut peptide preparation of high-quality.
(2) science is using the synergistic enzymolysis process binding protein of combination enzyme (tannase, cellulase, medium temperature amylase)
Qualitative change, centrifugation, washing process, on the one hand efficiently the walnut kernel skin in removal Walnut protein powder, starch, fiber, promotion digest
The purity of substrate Walnut protein powder, flavor, wherein tannase can effectively remove the color and luster and flavor effect of the generation of walnut kernel skin, fine
The plain enzyme of dimension and amylase can effectively degrade fiber in Walnut protein powder, starch, convenient in washing, centrifugation with albumen point
From；On the other hand appropriate denaturation treatment can effectively facilitate the separation of protein and impurity (mainly carbohydrate), beat simultaneously
The tight structure of walnut protein is opened, reduces its hydrophobicity, convenient for subsequently digesting, walnut peptide product yield is improved, improves product
Color and luster and flavor, low energy consumption for this method, it is easy to operate, be easy to realization of industrialization.
(3) because walnut protein Glutamic Acid, aspartic acid and arginine content are high, using alkalescence in enzymolysis process of the present invention
To improve enzymolysis efficiency and walnut peptide yield, pH value is adjusted using control initial pH value for protease, neutral proteinase stepwise discretization
And endpoint pH, the Action advantage of enzyme combination can be given full play to, and avoid introducing excessive acid solution, lye, reduce production cost.
(4) protein and macromolecular after digesting by centrifuging, remaining in plate compression removal centrifugal clear liquid
Peptide is enriched with small-molecular peptides ingredient, and simple for process compact, equipment cost is low, and production efficiency is high, easy realization of industrialization.
The present invention is by pre-processing, digesting, the preparation of industrialization height of the very economical property of the science of process for refining preferred implementation
The walnut peptide of purity, protein content more than 90%, peptide content more than 85%, relative molecular weight are less than 2000Dal peptide accountings
More than 85%, relative molecular weight is less than 1000Dal peptides accounting more than 50%, and superior product quality, bitter taste is weak, is easy to and other food
Dispensing is arranged in pairs or groups, and brain tonic and intelligence development, strengthen immunity effect protrude, and can be widely used in all kinds of foods as high-quality functional material
In product, health products.The high added value comprehensive utilization of the achievable walnut processing byproduct of the present invention, promotes walnut intensive processing enterprise
Economic benefit promotes walnut plantation, the benign cycle of processing.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
Embodiment 1
(1) the scattered of walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, protein
Content >=55%, fat content≤15%) be configured to mass percent be 10% walnut protein solution, with mixer with
The rotating speed of 2000rpm disperses Walnut protein powder, aquation 20min；
(2) tannase, cellulase, medium temperature amylase enzymolysis：The good walnut protein solution of scattered, aquation is heated to 50
DEG C, addition is equivalent to 0.15% tannase of walnut dregs quality (Hunan century Hua Xing bioengineering Co., Ltd), 0.5% cellulose
Enzyme (neutral, Beijing Xia Sheng biotechnologies development corporation, Ltd.), 1.0% medium temperature amylase (Hunan century China's star bioengineering have
Limit company), constant temperature stirring (36rpm) enzymolysis 60min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
1.0h is handled at 100 DEG C, centrifugal separator is used to centrifuge 15min with 4000r/min rotating speeds, removes fat and liquid, collects precipitation,
4 times of 50 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 6 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 8.0, adds in the alkali protease for being equivalent to protein quality 1.0% in walnut protein slurry
(Alcalase2.4L, letter (China) Investment Co., Ltd of Novi) adds in 55 DEG C of stirring hydrolysis 1.5h and is equivalent to walnut egg
(neutral proteinase Neutrase, Novi's letter (China) limited investment are public for the neutral proteinase of protein quality 1.0% in white slurry
Department) (36rpm) hydrolysis 8h are stirred in 55 DEG C, it is warming up to 90 DEG C of enzyme deactivation 30min after enzymolysis；
(5) centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation, after centrifugation
Clear liquid is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 30%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 180 DEG C, and outlet temperature control is 85 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Embodiment 2
(the scattered of (1) walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, albumen
Matter content >=55%, fat content≤15%) be configured to mass percent be 5% walnut protein solution, with mixer with
The rotating speed of 3000rpm disperses Walnut protein powder, aquation 10min；
(2) tannase, cellulase, medium temperature amylase enzymolysis：The good walnut protein solution of scattered, aquation is heated to 45
DEG C, addition is equivalent to 0.1% tannase of walnut dregs quality (Hunan century Hua Xing bioengineering Co., Ltd), 0.1% cellulose
Enzyme (neutral, Beijing Xia Sheng biotechnologies development corporation, Ltd.), 0.5% medium temperature amylase (Hunan century China's star bioengineering have
Limit company), constant temperature stirring (18rpm) enzymolysis 90min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
0.5h is handled at 121 DEG C, centrifugal separator is used to centrifuge 20min with 3000r/min rotating speeds, removes fat and liquid, collects precipitation,
3 times of 55 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 6 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 8.5, adds in the alkali protease for being equivalent to protein quality 0.5% in walnut protein slurry
(Alcalase2.4L, letter (China) Investment Co., Ltd of Novi) adds in 50 DEG C of stirring hydrolysis 2h and is equivalent to walnut protein
The neutral proteinase (neutral proteinase Neutrase, letter (China) Investment Co., Ltd of Novi) of protein quality 0.5% in slurry
85 DEG C of enzyme deactivation 40min are warming up to after 55 DEG C of stirring (18rpm) hydrolysis 16h, enzymolysis；
(5) centrifuge, centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation,
Clear liquid after centrifugation is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 20%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 170 DEG C, and outlet temperature control is 75 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Embodiment 3
(1) the scattered of walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, protein
Content >=55%, fat content≤15%) be configured to mass percent be 15% walnut protein solution, with mixer with
The rotating speed of 2000rpm disperses Walnut protein powder, aquation 20min；
(2) tannase, cellulase, medium temperature amylase enzymolysis：The good walnut protein solution of scattered, aquation is heated to 50
DEG C, addition is equivalent to 0.2% tannase of walnut dregs quality (Hunan century Hua Xing bioengineering Co., Ltd), 1.0% cellulose
Enzyme (neutral, Beijing Xia Sheng biotechnologies development corporation, Ltd.), 2.0% medium temperature amylase (Hunan century China's star bioengineering have
Limit company), constant temperature stirring (25rpm) enzymolysis 30min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
1.0h is handled at 100 DEG C, centrifugal separator is used to centrifuge 15min with 4000r/min rotating speeds, removes fat and liquid, collects precipitation,
5 times of 50 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 8 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 8.5, adds in the alkali protease for being equivalent to protein quality 2.0% in walnut protein slurry
(Alcalase2.4L, letter (China) Investment Co., Ltd of Novi) adds in 55 DEG C of stirring hydrolysis 1h and is equivalent to walnut protein
The neutral proteinase (neutral proteinase Neutrase, letter (China) Investment Co., Ltd of Novi) of protein quality 2.0% in slurry
90 DEG C of enzyme deactivation 30min are warming up to after 55 DEG C of stirring (25rpm) hydrolysis 4h, enzymolysis；
(5) centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation, after centrifugation
Clear liquid is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 45%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 190 DEG C, and outlet temperature control is 80 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Embodiment 4
(1) the scattered of walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, protein
Content >=55%, fat content≤15%) be configured to mass percent be 12% walnut protein solution, with mixer with
The rotating speed of 2000rpm disperses Walnut protein powder, aquation 20min；
(2) tannase, cellulase, medium temperature amylase enzymolysis：The good walnut protein solution of scattered, aquation is heated to 50
DEG C, addition is equivalent to 0.15% tannase of walnut dregs quality (Hunan century Hua Xing bioengineering Co., Ltd), 0.8% cellulose
Enzyme (neutral, Beijing Xia Sheng biotechnologies development corporation, Ltd.), 1.5% medium temperature amylase (Hunan century China's star bioengineering have
Limit company), constant temperature stirring (36rpm) enzymolysis 60min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
1.0h is handled at 100 DEG C, centrifugal separator is used to centrifuge 15min with 4000r/min rotating speeds, removes fat and liquid, collects precipitation,
4.5 times of 50 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 7 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 8.0, adds in the alkali protease for being equivalent to protein quality 1.0% in walnut protein slurry
(Alcalase2.4L, letter (China) Investment Co., Ltd of Novi) adds in 55 DEG C of stirring hydrolysis 2h and is equivalent to walnut protein
The neutral proteinase (neutral proteinase Neutrase, letter (China) Investment Co., Ltd of Novi) of protein quality 1.0% in slurry
90 DEG C of enzyme deactivation 30min are warming up to after 55 DEG C of stirring (36rpm) hydrolysis 8h, enzymolysis；
(5) centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation, after centrifugation
Clear liquid is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 40%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 200 DEG C, and outlet temperature control is 90 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Comparative example 1
(1) the scattered of walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, protein
Content >=55%, fat content≤15%) be configured to mass percent be 10% walnut protein solution, with mixer with
The rotating speed of 2000rpm disperses Walnut protein powder, aquation 20min；
(2) cellulase, medium temperature amylase enzymolysis：The good walnut protein solution of scattered, aquation is heated to 50 DEG C, is added in
It is equivalent to 0.5% cellulase of walnut dregs quality (neutral, Beijing Xia Sheng biotechnologies development corporation, Ltd.), 1.0% medium temperature is formed sediment
Powder enzyme (Hunan century Hua Xing bioengineering Co., Ltd), constant temperature stirring (36rpm) enzymolysis 60min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
1.0h is handled at 100 DEG C, centrifugal separator is used to centrifuge 15min with 4000r/min rotating speeds, removes fat and liquid, collects precipitation,
4 times of 50 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 6 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 8.0, adds in the alkali protease for being equivalent to protein quality 1.0% in walnut protein slurry
(Alcalase2.4L, letter (China) Investment Co., Ltd of Novi) adds in 55 DEG C of stirring hydrolysis 1.5h and is equivalent to walnut egg
(neutral proteinase Neutrase, Novi's letter (China) limited investment are public for the neutral proteinase of protein quality 1.0% in white slurry
Department) (36rpm) hydrolysis 8h are stirred in 55 DEG C, it is warming up to 90 DEG C of enzyme deactivation 30min after enzymolysis；
(5) centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation, after centrifugation
Clear liquid is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 30%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 180 DEG C, and outlet temperature control is 85 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Comparative example 2
(1) the scattered of walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, protein
Content >=55%, fat content≤15%) be configured to mass percent be 10% walnut protein solution, with mixer with
The rotating speed of 2000rpm disperses Walnut protein powder, aquation 20min；
(2) tannase, medium temperature amylase enzymolysis：The good walnut protein solution of scattered, aquation is heated to 50 DEG C, adds in phase
When in 0.15% tannase of walnut dregs quality (Hunan century Hua Xing bioengineering Co., Ltd), 1.0% medium temperature amylase (Hunan
Century Hua Xing bioengineering Co., Ltd), constant temperature stirring (36rpm) enzymolysis 60min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
1.0h is handled at 100 DEG C, centrifugal separator is used to centrifuge 15min with 4000r/min rotating speeds, removes fat and liquid, collects precipitation,
4 times of 50 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 6 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 8.0, adds in the alkali protease for being equivalent to protein quality 1.0% in walnut protein slurry
(Alcalase2.4L, letter (China) Investment Co., Ltd of Novi) adds in 55 DEG C of stirring hydrolysis 1.5h and is equivalent to walnut egg
(neutral proteinase Neutrase, Novi's letter (China) limited investment are public for the neutral proteinase of protein quality 1.0% in white slurry
Department) (36rpm) hydrolysis 8h are stirred in 55 DEG C, it is warming up to 90 DEG C of enzyme deactivation 30min after enzymolysis；
(5) centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation, after centrifugation
Clear liquid is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 30%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 180 DEG C, and outlet temperature control is 85 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Comparative example 3
(1) the scattered of walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, protein
Content >=55%, fat content≤15%) be configured to mass percent be 10% walnut protein solution, with mixer with
The rotating speed of 2000rpm disperses Walnut protein powder, aquation 20min；
(2) tannase, cellulase degradation：The good walnut protein solution of scattered, aquation is heated to 50 DEG C, is added in suitable
In 0.15% tannase of walnut dregs quality (Hunan century Hua Xing bioengineering Co., Ltd), 0.5% cellulase (neutral, north
Jing Xiasheng biotechnologies development corporation, Ltd.), constant temperature stirring (36rpm) enzymolysis 60min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
1.0h is handled at 100 DEG C, centrifugal separator is used to centrifuge 15min with 4000r/min rotating speeds, removes fat and liquid, collects precipitation,
4 times of 50 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 6 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 8.0, adds in the alkali protease for being equivalent to protein quality 1.0% in walnut protein slurry
(Alcalase2.4L, letter (China) Investment Co., Ltd of Novi) adds in 55 DEG C of stirring hydrolysis 1.5h and is equivalent to walnut egg
(neutral proteinase Neutrase, Novi's letter (China) limited investment are public for the neutral proteinase of protein quality 1.0% in white slurry
Department) (36rpm) hydrolysis 8h are stirred in 55 DEG C, it is warming up to 90 DEG C of enzyme deactivation 30min after enzymolysis；
(5) centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation, after centrifugation
Clear liquid is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 30%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 180 DEG C, and outlet temperature control is 85 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Comparative example 4
(1) the scattered of walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, protein
Content >=55%, fat content≤15%) be configured to mass percent be 10% walnut protein solution, with mixer with
The rotating speed of 2000rpm disperses Walnut protein powder, aquation 20min；
(2) tannase, medium temperature amylase enzymolysis：The good walnut protein solution of scattered, aquation is heated to 50 DEG C, adds in phase
When in 0.15% tannase of walnut dregs quality (Hunan century Hua Xing bioengineering Co., Ltd), 1.5% medium temperature amylase (Hunan
Century Hua Xing bioengineering Co., Ltd), constant temperature stirring (36rpm) enzymolysis 60min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
1.0h is handled at 100 DEG C, centrifugal separator is used to centrifuge 15min with 4000r/min rotating speeds, removes fat and liquid, collects precipitation,
4 times of 50 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 6 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 8.0, adds in the alkali protease for being equivalent to protein quality 1.0% in walnut protein slurry
(Alcalase2.4L, letter (China) Investment Co., Ltd of Novi) adds in 55 DEG C of stirring hydrolysis 1.5h and is equivalent to walnut egg
(neutral proteinase Neutrase, Novi's letter (China) limited investment are public for the neutral proteinase of protein quality 1.0% in white slurry
Department) (36rpm) hydrolysis 8h are stirred in 55 DEG C, it is warming up to 90 DEG C of enzyme deactivation 30min after enzymolysis；
(5) centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation, after centrifugation
Clear liquid is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 30%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 180 DEG C, and outlet temperature control is 85 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Comparative example 5
(1) the scattered of walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, protein
Content >=55%, fat content≤15%) be configured to mass percent be 10% walnut protein solution, with mixer with
The rotating speed of 2000rpm disperses Walnut protein powder, aquation 20min；
(2) tannase, cellulase, enzyme enzymolysis：The good walnut protein solution of scattered, aquation is heated to 50 DEG C, is added in
Be equivalent to 0.15% tannase of walnut dregs quality (Hunan century Hua Xing bioengineering Co., Ltd), 1.5% cellulase (in
Property, Beijing Xia Sheng biotechnologies development corporation, Ltd.), constant temperature stirring (36rpm) enzymolysis 60min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
1.0h is handled at 100 DEG C, centrifugal separator is used to centrifuge 15min with 4000r/min rotating speeds, removes fat and liquid, collects precipitation,
4 times of 50 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 6 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 8.0, adds in the alkali protease for being equivalent to protein quality 1.0% in walnut protein slurry
(Alcalase2.4L, letter (China) Investment Co., Ltd of Novi) adds in 55 DEG C of stirring hydrolysis 1.5h and is equivalent to walnut egg
(neutral proteinase Neutrase, Novi's letter (China) limited investment are public for the neutral proteinase of protein quality 1.0% in white slurry
Department) (36rpm) hydrolysis 8h are stirred in 55 DEG C, it is warming up to 90 DEG C of enzyme deactivation 30min after enzymolysis；
(5) centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation, after centrifugation
Clear liquid is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 30%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 180 DEG C, and outlet temperature control is 85 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Comparative example 6
(1) the scattered of walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, protein
Content >=55%, fat content≤15%) be configured to mass percent be 10% walnut protein solution, with mixer with
The rotating speed of 2000rpm disperses Walnut protein powder, aquation 20min；
(2) tannase, cellulase, medium temperature amylase enzymolysis：The good walnut protein solution of scattered, aquation is heated to 50
DEG C, addition is equivalent to 0.15% tannase of walnut dregs quality (Hunan century Hua Xing bioengineering Co., Ltd), 0.5% cellulose
Enzyme (neutral, Beijing Xia Sheng biotechnologies development corporation, Ltd.), 1.0% medium temperature amylase (Hunan century China's star bioengineering have
Limit company), constant temperature stirring (36rpm) enzymolysis 60min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
1.0h is handled at 90 DEG C, centrifugal separator is used to centrifuge 15min with 4000r/min rotating speeds, removes fat and liquid, collects precipitation,
4 times of 50 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 6 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 8.0, adds in the alkali protease for being equivalent to protein quality 1.0% in walnut protein slurry
(Alcalase2.4L, letter (China) Investment Co., Ltd of Novi) adds in 55 DEG C of stirring hydrolysis 1.5h and is equivalent to walnut egg
(neutral proteinase Neutrase, Novi's letter (China) limited investment are public for the neutral proteinase of protein quality 1.0% in white slurry
Department) (36rpm) hydrolysis 8h are stirred in 55 DEG C, it is warming up to 90 DEG C of enzyme deactivation 30min after enzymolysis；
(5) centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation, after centrifugation
Clear liquid is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 30%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 180 DEG C, and outlet temperature control is 85 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Comparative example 7
(1) the scattered of walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, protein
Content >=55%, fat content≤15%) be configured to mass percent be 10% walnut protein solution, with mixer with
The rotating speed of 2000rpm disperses Walnut protein powder, aquation 20min；
(2) tannase, cellulase, medium temperature amylase enzymolysis：The good walnut protein solution of scattered, aquation is heated to 50
DEG C, addition is equivalent to 0.15% tannase of walnut dregs quality (Hunan century Hua Xing bioengineering Co., Ltd), 0.5% cellulose
Enzyme (neutral, Beijing Xia Sheng biotechnologies development corporation, Ltd.), 1.0% medium temperature amylase (Hunan century China's star bioengineering have
Limit company), constant temperature stirring (36rpm) enzymolysis 60min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
1.0h is handled at 100 DEG C, centrifugal separator is used to centrifuge 15min with 4000r/min rotating speeds, removes fat and liquid, collects precipitation,
4 times of 50 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 6 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 8.0, adds in the alkali protease for being equivalent to protein quality 1.0% in walnut protein slurry
(Alcalase2.4L, letter (China) Investment Co., Ltd of Novi) rises after 55 DEG C of stirring (36rpm) hydrolysis 9.5h, enzymolysis
Temperature is to 90 DEG C of enzyme deactivation 30min；
(5) centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation, after centrifugation
Clear liquid is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 30%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 180 DEG C, and outlet temperature control is 85 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Comparative example 8
(1) the scattered of walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, protein
Content >=55%, fat content≤15%) be configured to mass percent be 10% walnut protein solution, with mixer with
The rotating speed of 2000rpm disperses Walnut protein powder, aquation 20min；
(2) tannase, cellulase, medium temperature amylase enzymolysis：The good walnut protein solution of scattered, aquation is heated to 50
DEG C, addition is equivalent to 0.15% tannase of walnut dregs quality (Hunan century Hua Xing bioengineering Co., Ltd), 0.5% cellulose
Enzyme (neutral, Beijing Xia Sheng biotechnologies development corporation, Ltd.), 1.0% medium temperature amylase (Hunan century China's star bioengineering have
Limit company), constant temperature stirring (36rpm) enzymolysis 60min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
1.0h is handled at 100 DEG C, centrifugal separator is used to centrifuge 15min with 4000r/min rotating speeds, removes fat and liquid, collects precipitation,
4 times of 50 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 6 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 7.0, adds in the neutral proteinase (neutral protein for being equivalent to protein quality 1.0% in walnut protein slurry
Enzyme Neutrase, letter (China) Investment Co., Ltd of Novi) it heats up after 55 DEG C of stirring (36rpm) hydrolysis 9.5h, enzymolysis
To 90 DEG C of enzyme deactivation 30min；
(5) centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation, after centrifugation
Clear liquid is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 30%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 180 DEG C, and outlet temperature control is 85 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Comparative example 9
(1) the scattered of walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, protein
Content >=55%, fat content≤15%) be configured to mass percent be 10% walnut protein solution, with mixer with
The rotating speed of 2000rpm disperses Walnut protein powder, aquation 20min；
(2) tannase, cellulase, medium temperature amylase enzymolysis：The good walnut protein solution of scattered, aquation is heated to 50
DEG C, addition is equivalent to 0.15% tannase of walnut dregs quality (Hunan century Hua Xing bioengineering Co., Ltd), 0.5% cellulose
Enzyme (neutral, Beijing Xia Sheng biotechnologies development corporation, Ltd.), 1.0% medium temperature amylase (Hunan century China's star bioengineering have
Limit company), constant temperature stirring (36rpm) enzymolysis 60min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
1.0h is handled at 100 DEG C, centrifugal separator is used to centrifuge 15min with 4000r/min rotating speeds, removes fat and liquid, collects precipitation,
4 times of 50 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 6 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 8.0, adds in the alkali protease for being equivalent to protein quality 2.0% in walnut protein slurry
(Alcalase2.4L, letter (China) Investment Co., Ltd of Novi) rises after 55 DEG C of stirring (36rpm) hydrolysis 9.5h, enzymolysis
Temperature is to 90 DEG C of enzyme deactivation 30min；
(5) centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation, after centrifugation
Clear liquid is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 30%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 180 DEG C, and outlet temperature control is 85 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Comparative example 10
(1) the scattered of walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, protein
Content >=55%, fat content≤15%) be configured to mass percent be 10% walnut protein solution, with mixer with
The rotating speed of 2000rpm disperses Walnut protein powder, aquation 20min；
(2) tannase, cellulase, medium temperature amylase enzymolysis：The good walnut protein solution of scattered, aquation is heated to 50
DEG C, addition is equivalent to 0.15% tannase of walnut dregs quality (Hunan century Hua Xing bioengineering Co., Ltd), 0.5% cellulose
Enzyme (neutral, Beijing Xia Sheng biotechnologies development corporation, Ltd.), 1.0% medium temperature amylase (Hunan century China's star bioengineering have
Limit company), constant temperature stirring (36rpm) enzymolysis 60min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
1.0h is handled at 100 DEG C, centrifugal separator is used to centrifuge 15min with 4000r/min rotating speeds, removes fat and liquid, collects precipitation,
4 times of 50 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 6 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 7.0, adds in the neutral proteinase (neutral protein for being equivalent to protein quality 2.0% in walnut protein slurry
Enzyme Neutrase, letter (China) Investment Co., Ltd of Novi) it heats up after 55 DEG C of stirring (36rpm) hydrolysis 9.5h, enzymolysis
To 90 DEG C of enzyme deactivation 30min；
(5) centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation, after centrifugation
Clear liquid is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 30%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 180 DEG C, and outlet temperature control is 85 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Comparative example 11
(1) the scattered of walnut dregs, aquation：With deionized water by the walnut dregs of cold pressing degreasing (in terms of butt, protein
Content >=55%, fat content≤15%) be configured to mass percent be 10% walnut protein solution, with mixer with
The rotating speed of 2000rpm disperses Walnut protein powder, aquation 20min；
(2) tannase, cellulase, medium temperature amylase enzymolysis：The good walnut protein solution of scattered, aquation is heated to 50
DEG C, addition is equivalent to 0.15% tannase of walnut dregs quality (Hunan century Hua Xing bioengineering Co., Ltd), 0.5% cellulose
Enzyme (neutral, Beijing Xia Sheng biotechnologies development corporation, Ltd.), 1.0% medium temperature amylase (Hunan century China's star bioengineering have
Limit company), constant temperature stirring (36rpm) enzymolysis 60min；
(3) protein denaturation, centrifugation, washing：By step (2), treated that walnut protein solution is pumped into tank, in temperature
1.0h is handled at 100 DEG C, centrifugal separator is used to centrifuge 15min with 4000r/min rotating speeds, removes fat and liquid, collects precipitation,
4 times of 50 DEG C of deionized waters are added, are stirred evenly, supernatant liquid is removed in centrifugation, collects precipitation；
(4) enzymolysis, enzyme deactivation：The precipitation that step (3) is obtained adds in 6 times of 55 DEG C of deionized waters, stirs evenly, and adjusts walnut
The pH value of protein milk is 8.0, adds in the alkali protease for being equivalent to protein quality 1.0% in walnut protein slurry
(Alcalase2.4L, letter (China) Investment Co., Ltd of Novi), 1.0% neutral proteinase (neutral proteinase Neutrase,
Novi believes (China) Investment Co., Ltd) it is warming up to 90 DEG C of enzyme deactivation 30min after 55 DEG C of stirring hydrolysis 9.5h, enzymolysis；
(5) centrifuge, plate compression：Walnut protein stock pump after enzyme deactivation is entered into decanter centrifuge centrifugation, after centrifugation
Clear liquid is again through plate-frame type diatomaceous earth filter press filtration；
(6) concentrate, is dry, packaging：Step (5) filtered solution is concentrated into solid 30%, then concentrate is subjected to spraying and is done
Dry, inlet temperature control is 180 DEG C, and outlet temperature control is 85 DEG C, and drying terminates, and packs to get to walnut peptide finished product.
Effect example
Walnut peptide powder made from Examples 1 to 4 and comparative example 1~11 is detected as follows：GB/T is respectively adopted
5492nd, GB/T 5009.3, GB/T 5009.4, GB/T 5009.5, method as defined in GB/T 5009.6 measure walnut peptide pink colour
Pool, moisture, ash content, protein content；3% solution is made in walnut peptide powder 50 DEG C of pure water of addition, its flavour is tasted, smells its smell；
Walnut peptide powder peptide content is analyzed using the method as defined in GB/T22729-2008 6.3；Using GB/T 22729-2008 annex
A high performance gel filtration chromatographies analysis walnut peptide powder relative molecular weight is less than 2000Dal, 1000Dal peptide proportion, the result is shown in
Table 1~3.
Walnut peptide powder made from 1 Examples 1 to 4 of table analyzes testing result
Note：Wherein walnut peptide powder made from Examples 1 to 4 is denoted as 1#, 2#, 3#, 4# respectively.
Walnut peptide powder made from 2 comparative example 1~5 of table analyzes testing result
Note：Wherein walnut peptide powder made from comparative example 1~5 is denoted as 5#, 6#, 7#, 8#, 9# respectively.
Walnut peptide powder made from 3 comparative example 6~11 of table analyzes testing result
Note：Wherein walnut peptide powder made from comparative example 6~10 is denoted as 10#, 11#, 12#, 13#, 14#, 15# respectively.
As table 1 as it can be seen that in walnut peptide made from using the method for the present invention Examples 1 to 4 protein mass percent for
91.88%~93.12% (>=90%), peptide content are 86.95%~88.21% (>=85%), and relative molecular weight is less than
>=85%, relative molecular weight is less than 1000Dal peptides accounting >=50% to 2000Dal peptides accounting, is good high purity protein
Peptide product.
By table 2~3 as it can be seen that compared with embodiment 1, comparative example 1 is not added with tannase, and product color yellow is deepened, and has hardship
Astringent taste, protein content, peptide content and relative molecular weight are less than 2000Dal, 1000Dal peptide accounting difference unobvious, it is seen that add
Add tannase that can improve the color and luster and flavor of product.Comparative example 2 is not added with cellulase, and comparative example 3 is not added with medium temperature amylase,
The light yellow intensification of product color is obtained, flavor and 1 difference of embodiment are little, and protein content, peptide content and relative molecular weight are small
Decline in 2000Dal, 1000Dal peptide accounting, the decline of comparative example 3 becomes apparent from, it is seen that addition cellulase, medium temperature amylase energy
Improve protein content, peptide content and the small-molecular peptides content of product.Comparative example 4 is not added with cellulase, and medium temperature amylase is used
Amount increases to 1.5%, obtains the light yellow intensification of product color, flavor and 2 difference of comparative example are little, protein content, peptide content
And relative molecular weight accounts for comparing embodiment 1 less than 2000Dal, 1000Dal peptide and declines, with 2 difference unobvious of comparative example；Comparison
Example 5 is not added with medium temperature amylase, and cellulase dosage increases to 1.5%, obtains the light yellow intensification of product color, flavor and comparison
3 difference of example is little, and protein content, peptide content and relative molecular weight account for comparing embodiment 1 less than 2000Dal, 1000Dal peptide
Decline, with 3 difference unobvious of comparative example；It can be seen that solely walnut protein substrate is removed using cellulase, medium temperature amylase
Carbon hydrate effect without both to be applied in combination effect good, apparent synergistic effect has been applied in combination.Comparative example 6 only changes walnut
Protein solution Denaturing is to handle 1.0h at 90 DEG C of temperature, and compared with Example 1, color and luster, flavor, protein content are without apparent
Variation, but peptide content and relative molecular weight are decreased obviously less than 2000Dal, 1000Dal peptide accounting, it is seen that cold press walnut dregs center
Peach albuminous degeneration degree has apparent influence to its enzymolysis product characteristic.Comparative example 7 is not added with neutral proteinase, and comparative example 8 is not
Addition alkali protease (initial enzymolysis pH is adjusted to 7.0), color and luster, flavor and protein content and 1 difference unobvious of embodiment,
But peptide content declines, and relative molecular weight is less than 2000Dal, 1000Dal peptide accounting and declines substantially, it is seen that complex enzyme is advantageously selected for
Improve peptide content and small-molecular peptides content.Comparative example 9 is not added with neutral proteinase, and basic protein enzyme dosage increases to 2.0%, production
Product color and luster, flavor, protein content and 1 difference of embodiment are little, and peptide content and relative molecular weight are less than 2000Dal, 1000Dal
Peptide accounts for comparing embodiment 1 and declines, with 7 difference unobvious of comparative example；Comparative example 10 is not added with alkali protease, neutral proteinase
Dosage increases to 2.0% (initial enzymolysis pH is adjusted to 7.0), and product color, flavor, protein content and 1 difference of embodiment are not
Greatly, peptide content and relative molecular weight account for comparing embodiment 1 less than 2000Dal, 1000Dal peptide and decline, with 8 difference of comparative example not
Substantially；Walnut peptide effect is prepared without the two it can be seen that solely being digested using alkali protease, neutral proteinase to walnut protein
It is good that effect is applied in combination, apparent synergistic effect has been applied in combination.11 neutral and alkali protease of comparative example, neutral proteinase are using same
Step enzymolysis, color and luster, flavor and protein content and 1 difference unobvious of embodiment, but peptide content declines, and relative molecular weight is less than
2000Dal, 1000Dal peptide accounting decline substantially, and peptide content and relative molecular weight connect less than 2000Dal, 1000Dal peptide accounting
Nearly comparative example 7, it is seen that complex enzyme order of addition influences substantially walnut peptide physicochemical property, and alkali protease, neutral proteinase are same
Step enzymolysis synergistic effect unobvious.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention and from above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
- It is 1. a kind of using cold pressing walnut dregs as the method for primary industry metaplasia production of high purity walnut peptide, it is characterised in that including with Lower step：(1) the scattered of walnut dregs, aquation：Cold press walnut dregs are weighed, walnut protein solution is formulated as with water；By walnut protein solution Shearing is uniformly dispersed, aquation；(2) tannase, cellulase, medium temperature amylase enzymolysis：By the good walnut protein solution of scattered, aquation be heated to 45 DEG C~ 55 DEG C, add in tannase, cellulase and medium temperature amylase, enzymolysis；The quality dosage of each enzyme is on the basis of walnut dregs quality It calculates, it is as follows：Tannase 0.1%~0.2%, cellulase 0.1%~1.0%, medium temperature amylase 0.5~2.0%；(3) protein denaturation, centrifugation, washing：Walnut protein solution after step (2) is digested is handled at 100~121 DEG C 0.5~1.0h, centrifugation discard supernatant liquid, collect precipitation, add the water that temperature is 50~60 DEG C, stir evenly, centrifugation is abandoned Supernatant liquid is removed, collects precipitation；(4) protease stepwise discretization, enzyme deactivation：The water that temperature is 50~60 DEG C is added in the precipitation obtained in step (3), stirring is equal It is even, obtain walnut protein slurry；The pH value for adjusting walnut protein slurry is 7.5~8.5, first adds in alkali protease in 50 DEG C~55 DEG C Stirring hydrolysis 1h~2h adds neutral proteinase in 50 DEG C~55 DEG C stirring hydrolysis 4h~16h；Enzyme deactivation after enzymolysis；Respectively The quality dosage of a enzyme by walnut protein starch in calculate on the basis of protein quality, it is as follows：Alkali protease 0.5%~2%, in Property proteinase-10 .5%~2%；(5) centrifuge, plate compression：Walnut protein enzymolysis product after enzyme deactivation is centrifuged, obtained clear liquid is through sheet frame Press filtration takes clarification filtered solution, obtains high-purity walnut peptide.
- 2. it is according to claim 1 using cold pressing walnut dregs as the method for primary industry metaplasia production of high purity walnut peptide, Characterized by further comprising following steps：(6) concentrate, is dry, packaging：The clarification filtered solution that step (5) is obtained concentrates, and concentrate is spray-dried, is obtained Walnut peptide finished product.
- It is 3. according to claim 1 or 2 using cold pressing walnut dregs as the side of primary industry metaplasia production of high purity walnut peptide Method, it is characterised in that：The concentration of walnut dregs is mass percent 5%~15% in walnut protein solution described in step (1)；The condition of shearing described in step (1) is 10~30min of speed shearing in 2000rpm~3000rpm.
- It is 4. according to claim 1 or 2 using cold pressing walnut dregs as the side of primary industry metaplasia production of high purity walnut peptide Method, it is characterised in that：The condition of enzymolysis described in step (2) is：Temperature is 45 DEG C~50 DEG C, the time is 30min~90min；Enzymolysis described in step (2) and step (4) is carried out under stirring, and mixing speed is less than 60rpm.
- It is 5. according to claim 1 or 2 using cold pressing walnut dregs as the side of primary industry metaplasia production of high purity walnut peptide Method, it is characterised in that：The condition of centrifugation described in step (3) is to centrifuge 10min~20min in 3000rpm~6000rpm；The dosage of water described in step (3) is equivalent to 3~5 times of the precipitation quality；The temperature of water described in step (3) is 50~55 DEG C；The dosage of water described in step (4) is equivalent to 3~8 times of the precipitation quality；The temperature of water described in step (4) is 55 DEG C；PH value described in step (4) is 8~8.5；The condition of enzyme deactivation described in step (4) is in 85~95 DEG C of 10~40min of enzyme deactivation.
- It is 6. according to claim 1 or 2 using cold pressing walnut dregs as the side of primary industry metaplasia production of high purity walnut peptide Method, it is characterised in that：Alkali protease described in step (4) is at least one of pancreatin, trypsase and bacillus licheniformis protease；Neutral proteinase described in step (4) believes neutral proteinase Neutrase, papain, bromelain for Novi At least one of with ficin.
- 7. it is according to claim 2 using cold pressing walnut dregs as the method for primary industry metaplasia production of high purity walnut peptide, It is characterized in that：The degree of concentration described in step (6) is 20~45% to be concentrated into solid content；To be dried by drying tower, operating parameter is as follows for drying described in step (6)：Inlet air temperature is 170 DEG C~200 DEG C, Leaving air temp is 75 DEG C~95 DEG C.
- 8. application of claim 1~7 any one of them method in industrialized production high-purity walnut peptide.
- 9. a kind of high-purity walnut peptide, it is characterised in that：It is prepared by claim 1~7 any one of them method；Institute The high-purity walnut peptide stated, walnut peptide protein content more than 90%, peptide content more than 85%, relative molecular weight is less than 2000Dal peptides accounting more than 85%, relative molecular weight are less than 1000Dal peptides accounting more than 50%.
- 10. the high-purity walnut peptide described in claim 9 produces the application in brain tonic and intelligence development, strengthen immunity product is prepared.
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| CN109527163A (en) * | 2018-11-21 | 2019-03-29 | 云南省林业科学院 | A kind of walnut polypeptide coffee and preparation method thereof |
| CN109757600A (en) * | 2019-02-13 | 2019-05-17 | 湖北瑞邦生物科技有限公司 | A kind of preparation method of purple perilla peptide |
| CN109845876A (en) * | 2018-12-28 | 2019-06-07 | 广州合诚实业有限公司 | Highly purified soybean oligopeptide and preparation method and application with unique solubility for high energy |
| CN110710592A (en) * | 2019-11-16 | 2020-01-21 | 四川农业大学 | Method for improving antioxidant activity of walnut cake protein |
| CN110819674A (en) * | 2019-11-14 | 2020-02-21 | 江南大学 | Method for preparing antihypertensive peptide by enzymolysis of walnut meal with compound protease |
| CN112753925A (en) * | 2021-01-27 | 2021-05-07 | 昆明生物制造研究院有限公司 | Preparation method of walnut kernel skin solid beverage |
| CN112841393A (en) * | 2020-12-30 | 2021-05-28 | 广州合诚实业有限公司 | Walnut peptide stable to zinc ions as well as preparation method and application thereof |
| CN112868877A (en) * | 2021-01-22 | 2021-06-01 | 昆明生物制造研究院有限公司 | Method for preparing walnut modified protein by using extrusion puffing technology |
| CN113057248A (en) * | 2021-04-14 | 2021-07-02 | 王朝辉 | Extraction method of walnut peptide |
| CN113854398A (en) * | 2021-10-08 | 2021-12-31 | 长春大学 | Method for preparing digestion-free walnut protein by using walnut cake meal as raw material |
| CN116076699A (en) * | 2021-11-05 | 2023-05-09 | 安琪生物科技有限公司 | Plant raw material meal enzymolysis product and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012149959A1 (en) * | 2011-05-03 | 2012-11-08 | Nestec S.A. | A hydrolysate of a protein substrate and a process for producing thereof |
| CN104293870A (en) * | 2014-09-25 | 2015-01-21 | 汾州裕源土特产品有限公司 | Preparation method of antioxidant walnut polypeptide health product |
| CN105002247A (en) * | 2015-07-30 | 2015-10-28 | 北京天肽生物科技有限公司 | Micromolecule walnut peptide and preparation method thereof |
| CN105063152A (en) * | 2015-09-10 | 2015-11-18 | 无限极（中国）有限公司 | Polypeptide raw material prepared by enzymolysis of walnuts and application thereof |
-
2017
- 2017-12-27 CN CN201711444692.9A patent/CN108085356B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012149959A1 (en) * | 2011-05-03 | 2012-11-08 | Nestec S.A. | A hydrolysate of a protein substrate and a process for producing thereof |
| CN104293870A (en) * | 2014-09-25 | 2015-01-21 | 汾州裕源土特产品有限公司 | Preparation method of antioxidant walnut polypeptide health product |
| CN105002247A (en) * | 2015-07-30 | 2015-10-28 | 北京天肽生物科技有限公司 | Micromolecule walnut peptide and preparation method thereof |
| CN105063152A (en) * | 2015-09-10 | 2015-11-18 | 无限极（中国）有限公司 | Polypeptide raw material prepared by enzymolysis of walnuts and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 张磊 等: "果汁中单宁脱除方法的研究进展", 《食品科学》 * |
| 邓欣伦 等: "核桃蛋白及其组分构象和功能特性的研究", 《现代食品科技》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109022525A (en) * | 2018-08-14 | 2018-12-18 | 吉林农业大学 | A kind of walnut active peptide and preparation method thereof improving memory function |
| CN109055472A (en) * | 2018-09-21 | 2018-12-21 | 滁州学院 | A kind of complex enzyme production method of walnut peptide |
| CN109527163A (en) * | 2018-11-21 | 2019-03-29 | 云南省林业科学院 | A kind of walnut polypeptide coffee and preparation method thereof |
| CN109845876A (en) * | 2018-12-28 | 2019-06-07 | 广州合诚实业有限公司 | Highly purified soybean oligopeptide and preparation method and application with unique solubility for high energy |
| CN109757600A (en) * | 2019-02-13 | 2019-05-17 | 湖北瑞邦生物科技有限公司 | A kind of preparation method of purple perilla peptide |
| CN110819674A (en) * | 2019-11-14 | 2020-02-21 | 江南大学 | Method for preparing antihypertensive peptide by enzymolysis of walnut meal with compound protease |
| CN110710592A (en) * | 2019-11-16 | 2020-01-21 | 四川农业大学 | Method for improving antioxidant activity of walnut cake protein |
| CN112841393A (en) * | 2020-12-30 | 2021-05-28 | 广州合诚实业有限公司 | Walnut peptide stable to zinc ions as well as preparation method and application thereof |
| CN112868877A (en) * | 2021-01-22 | 2021-06-01 | 昆明生物制造研究院有限公司 | Method for preparing walnut modified protein by using extrusion puffing technology |
| CN112753925A (en) * | 2021-01-27 | 2021-05-07 | 昆明生物制造研究院有限公司 | Preparation method of walnut kernel skin solid beverage |
| CN113057248A (en) * | 2021-04-14 | 2021-07-02 | 王朝辉 | Extraction method of walnut peptide |
| CN113854398A (en) * | 2021-10-08 | 2021-12-31 | 长春大学 | Method for preparing digestion-free walnut protein by using walnut cake meal as raw material |
| CN116076699A (en) * | 2021-11-05 | 2023-05-09 | 安琪生物科技有限公司 | Plant raw material meal enzymolysis product and preparation method and application thereof |
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