CN103404915B - Complex chelated fish skin bone protein polypeptide calcium powder and preparation method thereof - Google Patents
Complex chelated fish skin bone protein polypeptide calcium powder and preparation method thereof Download PDFInfo
- Publication number
- CN103404915B CN103404915B CN201310382788.2A CN201310382788A CN103404915B CN 103404915 B CN103404915 B CN 103404915B CN 201310382788 A CN201310382788 A CN 201310382788A CN 103404915 B CN103404915 B CN 103404915B
- Authority
- CN
- China
- Prior art keywords
- fish
- skin
- bone
- gained
- calcium powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Meat, Egg Or Seafood Products (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses complex chelated fish skin bone protein polypeptide calcium powder, belonging to the field of food processing methods. A preparation method of the complex chelated fish skin bone protein polypeptide calcium powder mainly comprises the steps: respectively digesting and pulping fish bones and fish skins, then mixing the obtained fish bone paste and fish skin pulp to obtain fish skin and bone paste; then adding water and compound protease for conducting enzymatic hydrolysis, and centrifuging the enzymatic hydrolysate to respectively obtain supernate and precipitate; and conducting airflow grinding on the precipitate to obtain ultramicro fish bone powder, conducting acidifying processing on the ultramicro fish bone powder in a lactic acid solution, then mixing fish bone calcium liquid with the supernate in a bioreactor, and conducting a chelating reaction to obtain the complex chelated fish skin bone protein polypeptide calcium powder. The complex chelated fish skin bone protein polypeptide calcium powder has a better antioxidant function; secondarily, the chelated calcium powder is good in solubility, and the biological calcium absorption rate is high; the chelated calcium powder provides collagen protein polypeptide which has the effects of enhancing the metabolism of cortical cells and preventing aging. The preparation method is simple and low in cost.
Description
Technical field
The invention belongs to functional food processing technique field, relate in particular to a kind of compound fish-skin SPP1 polypeptide chelate calcium powder, and the preparation method of this compound fish-skin SPP1 polypeptide chelate calcium powder.
Background technology
In fish-bone, calcium content is abundant, similar to the composition of skeleton calcium, is a kind of good natural calcium source; In fish-bone, also contain all amino acid that form human body protein; In addition, the content of mineral substances in fish-bone is significantly higher than other food, and in bone, the approximate 2:1 of calcium phosphorus ratio, is the optimal proportion of absorption of human body calcium phosphorus.At present, there are reports for processing and utilization to fish-bone and product development, utilize the complex enzyme of papain and neutral proteinase composition to carry out enzymolysis acquisition fishbone bioactive polypeptide calcium powder to fish-bone as patent " a kind of fishbone bioactive polypeptide calcium powder and preparation method " (publication number is: CN101731666A) discloses, patent " production method of a kind of instant bone calcium powder composition and products thereof " (publication number is: CN1344542A) discloses utilizes the complex enzymes such as papain snake bone, eel bone to be carried out to the bone calcium powder of enzymolysis gained; Patent " a kind of method of utilizing channel catfish fish-bone to prepare bone mud " (publication number is: 101422258A) discloses the method for utilizing alkali protease enzymolysis fish-bone to produce bone mud; Patent " utilizing Tilapia bone to produce the processing technology of calcium activated " (publication number is: CN1557344A) discloses and has utilized 1398 protease hydrolyzed fish-bones, and then obtains the method for calcium activated with acid treatment.But above-mentionedly just solely utilize protease to carry out enzymolysis to fish-bone.
In fish-skin, containing rich in protein, and major part be collagen, and fish-skin is prepared to collagen polypeptide by enzymolysis, has special physiological activity, has reinforcement cortical cell metabolism and prevent old and feeble effect.At present, the existing report that utilizes enzymolysis collagen, as patent " antioxidation polypeptide and the preparation method that utilize acid protease enzymolysis sharkskin collagen to prepare ", (application number is: 201110128242) disclose the method for utilizing acid protease enzymolysis shark fish-skin to prepare antioxidation polypeptide; Utilize the enzymolysis Java tilapia skin such as alkali protease obtain collagen enzymolysis liquids method (Zhang Hanjun etc. .2008.13.27-30 brewages in China).In addition, also have the red non-crucian skin of enzymolysis method (Zeng Mingyong etc. Chinese Tissue Engineering Study and the 2nd phase of clinical rehabilitation .2007).The shortcoming of said method is only fish-skin to be carried out to monistic enzymolysis, to the collagen polypeptide in fish-skin is used.
From above-mentioned result for retrieval, also synchronously do not utilize at present collagen polypeptide in the fish-skin report in conjunction with the compound fish-skin SPP1 polypeptide chelate calcium powder of fish-bone exploitation.
Summary of the invention
In order to effectively utilize the functional characteristic of fish-skin and fish-bone, the object of the present invention is to provide a kind of compound fish-skin SPP1 polypeptide chelate calcium powder.
Another object of the present invention is to provide the preparation method of above-mentioned compound fish-skin SPP1 polypeptide chelate calcium powder.
The present invention's the 3rd object is to provide the purposes of above-mentioned compound fish-skin SPP1 polypeptide chelate calcium powder.
For achieving the above object, technical scheme of the present invention is as follows:
A kind of compound fish-skin SPP1 polypeptide chelate calcium powder, is prepared as follows:
(1), freezing fish-bone is thawed, remove fish-bone remained on surface the flesh of fish, clean up; Fish-bone is boiled to 30～50min in the water of 100 DEG C, then fish-bone is pulled an oar with tissue mashing machine, through cooling, drain, obtain fish-bone mud; Be placed in-3～-5 DEG C of preservations;
(2), freezing fish-skin is thawed, clean up; Fish-skin is boiled to 25～35min in the water of 80～90 DEG C, then fish-skin is pulled an oar with tissue mashing machine, through cooling, drain, obtain fish-skin slurry, be placed in-3～-5 DEG C of preservations;
(3) the ratio mixing, by the fish-skin slurry of the fish-bone mud of step (1) gained and step (2) gained with 1:1, stirs 5～8min, obtains fish-skin bone mud, is placed in-3～-5 DEG C of preservations;
(4), according to the ratio that between water and fish-skin bone mud, mass ratio is 1.0～1.5:1, in the fish-skin bone mud of step (3) gained, add water, stir 5～8min, obtain fish-skin pulp; Then in fish-skin pulp, add compound protease according to the ratio that between compound protease and fish-skin pulp, mass ratio is 1:400～600, then under 45～60 DEG C, pH6.0～7.5 condition, carry out enzyme digestion reaction 30～45min; At 85～90 DEG C, be incubated 5～8 minutes again, then centrifugal 3～5min under room temperature, 3000～5000g condition, degrease, gets respectively supernatant and sediment;
(5), by the cleaning of sediment water, oven dry, the air-flow crushing of the centrifugal gained of step (4), obtain ultra micro fishbone dust; According to 1g:1～3mL w/v between ultra micro fishbone dust and lactic acid solution, ultra micro fishbone dust is placed in to lactic acid solution and carries out acidification 60～70min, obtain fish-bone calcium liquid;
(6), the fish-bone calcium liquid of the supernatant of step (4) gained and step (5) gained is mixed in bioreactor, stir 3～5min, then at 55～65 DEG C, under the condition of pH6.5～7.5, in bioreactor, stir chelatropic reaction 15～25min, freeze drying, obtains compound fish-skin SPP1 polypeptide chelate calcium powder.
Compound protease described in above-mentioned fish-skin SPP1 polypeptide chelate calcium powder step (4) is made up of Alcalase2.4L, AS1398 neutral proteinase and flavor protease, wherein Alcalase2.4L:AS1398 neutral proteinase: the ratio between flavor protease is 1:0.5～1.0:1.0～2.0.
The particle diameter of ultra micro fishbone dust described in above-mentioned compound fish-skin SPP1 polypeptide chelate calcium powder step (5) is 10～50um.
The concentration of volume percent of the lactic acid solution described in above-mentioned compound fish-skin SPP1 polypeptide chelate calcium powder step (5) is 30～40%.
The preparation method of above-mentioned compound fish-skin SPP1 polypeptide chelate calcium powder, carries out in accordance with the following steps:
(1), freezing fish-bone is thawed, remove fish-bone remained on surface the flesh of fish, clean up; Fish-bone is boiled to 30～50min in the water of 100 DEG C, then fish-bone is pulled an oar with tissue mashing machine, through cooling, drain, obtain fish-bone mud; Be placed in-3～-5 DEG C of preservations;
(2), freezing fish-skin is thawed, clean up; Fish-skin is boiled to 25～35min in the water of 80～90 DEG C, then fish-skin is pulled an oar with tissue mashing machine, through cooling, drain, obtain fish-skin slurry, be placed in-3～-5 DEG C of preservations;
(3) the ratio mixing, by the fish-skin slurry of the fish-bone mud of step (1) gained and step (2) gained with 1:1, stirs 5～8min, obtains fish-skin bone mud, is placed in-3～-5 DEG C of preservations;
(4), according to the ratio that between water and fish-skin bone mud, mass ratio is 1.0～1.5:1, in the fish-skin bone mud of step (3) gained, add water, stir 5～8min, obtain fish-skin pulp; Then in fish-skin pulp, add compound protease according to the ratio that between compound protease and fish-skin pulp, mass ratio is 1:400～600, then under 45～60 DEG C, pH6.0～7.5 condition, carry out enzyme digestion reaction 30～45min; At 85～90 DEG C, be incubated 5～8 minutes again, then centrifugal 3～5min under room temperature, 3000～5000g condition, degrease, gets respectively supernatant and sediment;
(5), by the cleaning of sediment water, oven dry, the air-flow crushing of the centrifugal gained of step (4), obtain ultra micro fishbone dust; According to 1g:1～3mL w/v between ultra micro fishbone dust and lactic acid solution, ultra micro fishbone dust is placed in to lactic acid solution and carries out acidification 60～70min, obtain fish-bone calcium liquid;
(6), the fish-bone calcium liquid of the supernatant of step (4) gained and step (5) gained is mixed in bioreactor, stir 3～5min, then at 55～65 DEG C, under the condition of pH6.5～7.5, in bioreactor, stir chelatropic reaction 15～25min, freeze drying, obtains compound fish-skin SPP1 polypeptide chelate calcium powder.
Compound protease described in above-mentioned preparation method's step (4) is made up of Alcalase2.4L, AS1398 neutral proteinase and flavor protease, wherein Alcalase2.4L:AS1398 neutral proteinase: the ratio between flavor protease is 1:0.5～1.0:1.0～2.0.
The particle diameter of ultra micro fishbone dust described in above-mentioned preparation method's step (5) is 10～50um.
The concentration of volume percent of the lactic acid solution described in above-mentioned preparation method's step (5) is 30～40%.
The compound fish-skin SPP1 of the present invention polypeptide chelate calcium powder is as the application on food additives.
The compound fish-skin SPP1 of the present invention polypeptide chelate calcium powder also can be used as nutraceutical.
Compared with prior art, the present invention has following advantage and beneficial effect: (1) fish-skin SPP1 of the present invention polypeptide chelate calcium powder has good anti-oxidation function characteristic, mainly due to the compound protease that utilizes Alcalase2.4L, AS1398 neutral proteinase and flavor protease composition, fish-skin and fish-bone is carried out containing collagen polypeptide and fish-bone polypeptide in fish-skin SPP1 polypeptide chelate calcium powder that enzymolysis obtains.(2) the present invention, to the calcium in fish-bone by acidification, then passes through air-flow ultramicro grinding, and the dissolubility of gained fish-skin SPP1 polypeptide chelate calcium powder is good, and dissolution rate reaches more than 95%, and can reach more than 73% the biological absorption of calcium wherein.(3) fish-skin SPP1 polypeptide chelate calcium powder of the present invention is the compound protease enzymolysis through three kinds of protease compositions, then obtains through ultra micro air-flow crushing, is free from side effects, and has light taste with sweet and sour flavor, has good local flavor.(4) fish-skin SPP1 polypeptide chelate calcium powder of the present invention directly absorbs from intestinal mucosa with the integral form of polypeptide, thereby efficiently solve, traditional calcium source is poorly soluble, absorptivity is low, side effect is large, produce the problems such as calculus.(5) with traditional calcium-supplementing preparation as compared with calcium carbonate, calcium gluconae, calcium acetate, calcium lactate etc., fish-skin SPP1 polypeptide chelate calcium powder of the present invention can meet the needs of life entity to calcium constituent, the reinforcement cortical cell metabolism of collagen polypeptide can be provided again and prevent old and feeble effect.(6) preparation method of the present invention is simple, and cost is low.
Detailed description of the invention
The preparation of the compound fish-skin SPP1 of embodiment 1 the present invention polypeptide chelate calcium powder
(1) 1000 grams, freezing cod bone is thawed, remove remained on surface the flesh of fish, clean up; Boiled fish bone 50min in 100 DEG C of water, pulls an oar fish-bone with tissue mashing machine, through cooling, drain, obtain 700 grams, fish-bone mud; Be placed in-5 DEG C of preservations;
(2) 1000 grams, freezing fish-skin is thawed, clean up; In 90 DEG C of water, boil 25min, fish-skin pulled an oar with tissue mashing machine, through cooling, drain, obtain 750 grams, fish-skin slurry, be placed in-5 DEG C of preservations;
(3) by 500 grams of mixing of fish-skin slurry of 500 grams, the fish-bone mud of step (1) gained and step (2) gained, stir 8min, obtain 1000 grams, fish-skin bone mud, be placed in-5 DEG C of preservations.
(4) according to the ratio that between water and fish-skin bone mud, mass ratio is 1.5:1, in the fish-skin bone mud of step (3) gained, add 1500 grams, water, stir 8min, obtain fish-skin pulp; In fish-skin pulp, adding 2 grams of compound proteases according to the ratio that between compound protease (Alcalase2.4L:AS1398 neutral proteinase: flavor protease=1:0.5:1.0) and fish-bone slurry, mass ratio is 1:500, is enzymolysis 45min under 7.0 conditions at 50 DEG C, pH; At 90 DEG C, be incubated 5 minutes again, centrifugal 5min under cool to room temperature, 3000g condition, degrease, gets respectively 1150 grams of 1300 grams of supernatants and sediments.
(5) by the sediment water cleaning of step (4) gained, oven dry, air-flow crushing (spiral air flow mill 50AS), obtain 162 grams of ultra micro fishbone dusts; According to 1g:2mL w/v between fishbone dust and lactic acid solution, ultra micro fishbone dust is placed in to 324ml lactic acid solution and carries out acidification 60min, obtain fish-bone calcium liquid; The concentration of volume percent of described lactic acid solution is 40%.
(6) the fish-bone calcium liquid of the supernatant of step (4) gained and step (5) gained is mixed in bioreactor, stir 5min, then at 65 DEG C, under pH7.0 condition, in bioreactor, stir chelatropic reaction 25min, freeze drying (FD-1PF, Beijing De Tianyou development in science and technology Co., Ltd produces), obtains 306 grams of the compound fish-skin SPP1 of the present invention polypeptide chelate calcium powders.
The preparation of the compound fish-skin SPP1 of embodiment 2 the present invention polypeptide chelate calcium powder
(1) 500 grams, freezing cod bone is thawed, remove remained on surface the flesh of fish, clean up; Fish-bone is boiled to 40min in the water of 100 DEG C, fish-bone is pulled an oar with tissue mashing machine, through cooling, drain, obtain 360 grams, fish-bone mud; Be placed in-3 DEG C of preservations.
(2) 500 grams, freezing fish-skin is thawed, clean up; Fish-skin is boiled to 25min in the water of 80 DEG C, fish-skin is pulled an oar with tissue mashing machine, through cooling, drain, obtain 380 grams, fish-skin slurry, be placed in-3 DEG C of preservations.
(3) get 250 grams of mixing of fish-bone mud that 250 grams, fish-bone mud that step (1) obtains and step (2) obtain, stir 7min, obtain 500 grams, fish-skin bone mud, be placed in-3 DEG C of preservations.
(4) according to the ratio that between water and fish-skin bone mud, mass ratio is 1.3:1, in the fish-skin bone mud of step (3) gained, add 650 grams of water, stir 8min, obtain fish-skin pulp; In fish-skin pulp, adding 1 gram of compound protease according to the ratio that between compound protease (Alcalase2.4L:AS1398 neutral proteinase: flavor protease=1:1:2) and fish-skin pulp, mass ratio is 1:500, is enzymolysis 40min under 7.5 conditions at 55 DEG C, pH; At 88 DEG C, be incubated 8 minutes again, centrifugal 4min under cool to room temperature, 4000g condition, degrease, gets respectively 500 grams of 630 grams of supernatants and sediments.
(5) by the sediment water cleaning of the centrifugal gained of step (4), oven dry, air-flow crushing (spiral air flow mill 50AS), obtain 82 grams of ultra micro fishbone dusts; According to 1g:3mL w/v between ultra micro fishbone dust and lactic acid solution, ultra micro fishbone dust is placed in to 246ml lactic acid solution and carries out acidification 65min, obtain fish-bone calcium liquid; The concentration of volume percent of described lactic acid solution is 30%.
(6) the fish-bone calcium liquid of the supernatant of step (4) gained and step (5) gained is mixed in bioreactor, stir 5min, then under 60 DEG C, pH6.5 condition, in bioreactor, stir chelatropic reaction 20min, freeze drying (FD-1PF, Beijing De Tianyou development in science and technology Co., Ltd), obtain 149 grams of the compound fish-skin SPP1 of the present invention polypeptide chelate calcium powders.
The preparation of the compound fish-skin SPP1 of embodiment 3 the present invention polypeptide chelate calcium powder
(1) 100 grams, freezing cod bone is thawed, remove remained on surface the flesh of fish, clean up; Fish-bone is boiled to 30min in the water of 100 DEG C, fish-bone is pulled an oar with tissue mashing machine, through cooling, drain, obtain 76 grams, fish-bone mud; Be placed in-3 DEG C of preservations.
(2) 100 grams, freezing fish-skin is thawed, clean up; Fish-skin is boiled to 35min in the water of 85 DEG C, fish-skin is pulled an oar with tissue mashing machine, through cooling, drain, obtain 380 grams, fish-skin slurry, be placed in-3 DEG C of preservations.
(3) by 50 grams of mixing of fish-skin slurry of 50 grams, the fish-bone mud of step (1) gained and step (2) gained, stir 5min, obtain 100 grams, fish-skin bone mud, be placed in-5 DEG C of preservations.
(4) according to the ratio that between water and fish-skin bone mud, mass ratio is 1.2:1, in the fish-skin bone mud of step (3) gained, add 120 grams, water, stir 8min, obtain fish-skin pulp; In fish-skin pulp, adding 0.25 gram of compound protease according to the ratio that between compound protease (Alcalase2.4L:AS1398 neutral proteinase: flavor protease=1:0.5:2) and fish-bone slurry, mass ratio is 1:400, is enzymolysis 45min under 6.5 conditions at 60 DEG C, pH; At 85 DEG C, be incubated 8 minutes again, centrifugal 5min under cool to room temperature, 3000g condition, degrease, gets respectively 102 grams of 110 grams of supernatants and sediments.
(5) by the sediment water cleaning of step (4) gained, oven dry, air-flow crushing (spiral air flow mill 50AS), obtain 15.8 grams of ultra micro fishbone dusts; According to 1g:2mL w/v between ultra micro fishbone dust and lactic acid solution, ultra micro fishbone dust is placed in to 31.6ml lactic acid solution and carries out acidification 70min, obtain fish-bone calcium liquid; The concentration of volume percent of described lactic acid solution is 35%.
(6) the fish-bone calcium liquid of the supernatant of step (4) gained and step (5) gained is mixed in bioreactor, stir 4min, then under 55 DEG C, pH7.0 condition, in bioreactor, stir chelatropic reaction 15min, freeze drying (FD-1PF, Beijing De Tianyou development in science and technology Co., Ltd), obtain 29.8 grams of the compound fish-skin SPP1 of the present invention polypeptide chelate calcium powders.
The determination test of calcium biological absorption in the compound fish-skin SPP1 of embodiment 4 the present invention polypeptide chelate calcium powder
Carry out as follows:
Get 50 of experiment Wistar rats, every body weight 185～200g, basal feed adaptability is fed 1 week, is then divided at random 5 groups:
A group: basal feed (basal feed constituent and percentage by weight thereof: casein 20%, cornstarch 65%, corn oil 8%, cellulose 1%, B B-complex 1%, complex inorganic salt catalyst 5%);
The compound fish-skin SPP1 polypeptide chelate calcium powder 5g/ (kgd) of B group: basal feed+embodiment 1;
The compound fish-skin SPP1 polypeptide chelate calcium powder 5g/ (kgd) of C group: basal feed+embodiment 2;
The compound fish-skin SPP1 polypeptide chelate calcium powder 5g/ (kgd) of D group: basal feed+embodiment 3;
E group: basal feed+common fish-bone calcium powder 5g/ (kgd);
In basal feed, calcium content actual measurement is 1166mg/kg.
Each group rat is under equal conditions raised, and freely ingests, weigh weekly 1 time, and every day entry food-intake.After the 4th week, move into metabolic cage, excrement, urine are collected in the calcium metabolism experiment of carrying out 3 days.
Calcium absorptivity computing formula is:
Calcium absorptivity (%)=(calcium intake one excrement calcium discharge rate)/calcium intake × 100%.
The determination test result of calcium biological absorption in the compound fish-skin SPP1 of table 1 polypeptide chelate calcium powder
| Test group | Calcium absorptivity (%) |
| A group | 28.8±2.28 |
| B group | 73.48±3.38 |
| C group | 74.5±2.45 |
| D group | 75.1±3.06 |
| E group | 48.07±4.23 |
In the compound fish-skin SPP1 polypeptide chelate calcium powder that result (in table 1) the present invention produces, calcium biological absorption, all more than 73%, is significantly higher than common fish-bone calcium powder and control group.Illustrate that in the compound fish-skin SPP1 of the present invention polypeptide chelate calcium powder, calcium biological absorption is high.
The compound fish-skin SPP1 of embodiment 5 the present invention polypeptide chelate calcium powder DPPH radicals scavenging power determination test
Carry out as follows:
Test specimen: the fish-skin SPP1 polypeptide chelate calcium powder of the single fish-bone polypeptide chelating calcium powder (contrast) of preparation, embodiment 1, embodiment 2, embodiment 3
The mensuration of DPPH radicals scavenging power adopts Shimada et al.(1992) method.Sample solution 1.5ml(0.5-10mg/mL) mix with the DPPH ethanolic solution 1.5ml of 0.1mM, vibration mixes, 25 DEG C of (room temperature) lucifuge water-bath 30min, after under 517nm detection architecture light absorption value.Adopt following formula to calculate the vigor (valid density of sample when EC50 value representation DPPH free radical is eliminated 50%) of removing DPPH free radical:
DPPH free radical scavenging activity (%)=(1-A contrast/A sample) × 100%
Table 2 fish-skin SPP1 polypeptide chelate calcium powder DPPH radicals scavenging power result of the test
| Sample source | DPPH radicals scavenging power EC50(mg/ml) |
| Embodiment 1 | 1.65±0.02 |
| Embodiment 2 | 1.78±0.03 |
| Embodiment 3 | 1.71±0.04 |
| Fish-bone polypeptide chelating calcium powder | 9.23±0.72 |
The remarkable EC50 value lower than fish-bone polypeptide chelating calcium powder of EC50 value of the compound fish-skin SPP1 polypeptide chelate calcium powder that result (in table 2) the present invention produces, the DPPH radicals scavenging power that the compound fish-skin SPP1 polypeptide chelate calcium powder of the present invention's production is described is significantly better than fish-bone polypeptide chelating calcium powder, this is mainly to have certain fish-skin polypeptide powder in fish-skin SPP1 polypeptide chelate calcium powder of the present invention, illustrates that compound fish-skin SPP1 polypeptide chelate calcium powder prepared by the present invention has good DPPH radicals scavenging power ability.
Claims (3)
1. a compound fish-skin SPP1 polypeptide chelate calcium powder, is characterized in that being prepared as follows:
(1), freezing fish-bone is thawed, remove fish-bone remained on surface the flesh of fish, clean up; Fish-bone is boiled to 30～50min in the water of 100 DEG C, then fish-bone is pulled an oar with tissue mashing machine, through cooling, drain, obtain fish-bone mud; Be placed in-3～-5 DEG C of preservations;
(2), freezing fish-skin is thawed, clean up; Fish-skin is boiled to 25～35min in the water of 80～90 DEG C, then fish-skin is pulled an oar with tissue mashing machine, through cooling, drain, obtain fish-skin slurry, be placed in-3～-5 DEG C of preservations;
(3) the ratio mixing, by the fish-skin slurry of the fish-bone mud of step (1) gained and step (2) gained with 1:1, stirs 5～8min, obtains fish-skin bone mud, is placed in-3～-5 DEG C of preservations;
(4), according to the ratio that between water and fish-skin bone mud, mass ratio is 1.0～1.5:1, in the fish-skin bone mud of step (3) gained, add water, stir 5～8min, obtain fish-skin pulp; Then in fish-skin pulp, add compound protease according to the ratio that between compound protease and fish-skin pulp, mass ratio is 1:400～600, then under 45～60 DEG C, pH6.0～7.5 condition, carry out enzyme digestion reaction 30～45min; At 85～90 DEG C, be incubated 5～8 minutes again, then centrifugal 3～5min under room temperature, 3000～5000g condition, degrease, gets respectively supernatant and sediment; Wherein said compound protease is made up of Alcalase2.4L, AS1398 neutral proteinase and flavor protease, wherein Alcalase2.4L:AS1398 neutral proteinase: the ratio between flavor protease is 1:0.5～1.0:1.0～2.0;
(5), by the cleaning of sediment water, oven dry, the air-flow crushing of the centrifugal gained of step (4), obtain ultra micro fishbone dust; According to 1g:1～3mL w/v between ultra micro fishbone dust and lactic acid solution, ultra micro fishbone dust is placed in to lactic acid solution and carries out acidification 60～70min, obtain fish-bone calcium liquid; The particle diameter of wherein said ultra micro fishbone dust is 10～50um; The concentration of volume percent of described lactic acid solution is 30～40%;
(6), the fish-bone calcium liquid of the supernatant of step (4) gained and step (5) gained is mixed in bioreactor, stir 3～5min, then at 55～65 DEG C, under the condition of pH6.5～7.5, in bioreactor, stir chelatropic reaction 15～25min, freeze drying, obtains compound fish-skin SPP1 polypeptide chelate calcium powder.
2. the preparation method of compound fish-skin SPP1 polypeptide chelate calcium powder claimed in claim 1, is characterized in that carrying out in accordance with the following steps:
(1), freezing fish-bone is thawed, remove fish-bone remained on surface the flesh of fish, clean up; Fish-bone is boiled to 30～50min in the water of 100 DEG C, then fish-bone is pulled an oar with tissue mashing machine, through cooling, drain, obtain fish-bone mud; Be placed in-3～-5 DEG C of preservations;
(2), freezing fish-skin is thawed, clean up; Fish-skin is boiled to 25～35min in the water of 80～90 DEG C, then fish-skin is pulled an oar with tissue mashing machine, through cooling, drain, obtain fish-skin slurry, be placed in-3～-5 DEG C of preservations;
(3) the ratio mixing, by the fish-skin slurry of the fish-bone mud of step (1) gained and step (2) gained with 1:1, stirs 5～8min, obtains fish-skin bone mud, is placed in-3～-5 DEG C of preservations;
(4), according to the ratio that between water and fish-skin bone mud, mass ratio is 1.0～1.5:1, in the fish-skin bone mud of step (3) gained, add water, stir 5～8min, obtain fish-skin pulp; Then in fish-skin pulp, add compound protease according to the ratio that between compound protease and fish-skin pulp, mass ratio is 1:400～600, then under 45～60 DEG C, pH6.0～7.5 condition, carry out enzyme digestion reaction 30～45min; At 85～90 DEG C, be incubated 5～8 minutes again, then centrifugal 3～5min under room temperature, 3000～5000g condition, degrease, gets respectively supernatant and sediment; Wherein said compound protease is made up of Alcalase2.4L, AS1398 neutral proteinase and flavor protease, wherein Alcalase2.4L:AS1398 neutral proteinase: the ratio between flavor protease is 1:0.5～1.0:1.0～2.0;
(5), by the cleaning of sediment water, oven dry, the air-flow crushing of the centrifugal gained of step (4), obtain ultra micro fishbone dust; According to 1g:1～3mL w/v between ultra micro fishbone dust and lactic acid solution, ultra micro fishbone dust is placed in to lactic acid solution and carries out acidification 60～70min, obtain fish-bone calcium liquid; The particle diameter of wherein said ultra micro fishbone dust is 10～50um; The concentration of volume percent of described lactic acid solution is 30～40%;
(6), the fish-bone calcium liquid of the supernatant of step (4) gained and step (5) gained is mixed in bioreactor, stir 3～5min, then at 55～65 DEG C, under the condition of pH6.5～7.5, in bioreactor, stir chelatropic reaction 15～25min, freeze drying, obtains compound fish-skin SPP1 polypeptide chelate calcium powder.
3. compound fish-skin SPP1 polypeptide chelate calcium powder claimed in claim 1 is as the application of food additives.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310382788.2A CN103404915B (en) | 2013-08-27 | 2013-08-27 | Complex chelated fish skin bone protein polypeptide calcium powder and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310382788.2A CN103404915B (en) | 2013-08-27 | 2013-08-27 | Complex chelated fish skin bone protein polypeptide calcium powder and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103404915A CN103404915A (en) | 2013-11-27 |
| CN103404915B true CN103404915B (en) | 2014-11-26 |
Family
ID=49598005
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310382788.2A Active CN103404915B (en) | 2013-08-27 | 2013-08-27 | Complex chelated fish skin bone protein polypeptide calcium powder and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103404915B (en) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103610157A (en) * | 2013-12-06 | 2014-03-05 | 合肥工业大学 | Method for producing fishbone enzymatic pottage by utilizing fishbone |
| CN103710403B (en) * | 2013-12-09 | 2016-05-04 | 广东环西生物科技股份有限公司 | Compound amino acid chelate calcium high-efficiency cleaning production technology |
| CN104232719B (en) * | 2014-09-30 | 2017-01-25 | 中国海洋大学 | Method for preparing calcium chelated peptide |
| CN104958756B (en) * | 2015-06-05 | 2018-07-20 | 孝感顺利特种养殖有限公司 | A kind of chelated calcium preparation method of crocodile ossein |
| CN105249464B (en) * | 2015-11-18 | 2018-02-23 | 江南大学 | The one chelated calcium preparation method of seed shrimp peptide |
| CN106689718A (en) * | 2016-12-21 | 2017-05-24 | 安徽强农牧业有限公司 | Composite chelating calcium powder prepared from processed hawthorn for dairy cow |
| CN106689719A (en) * | 2016-12-21 | 2017-05-24 | 安徽强农牧业有限公司 | Composite chelated calcium powder prepared from baking black soya beans for dairy cattle |
| CN106615741A (en) * | 2016-12-21 | 2017-05-10 | 安徽强农牧业有限公司 | Composite chelated calcium powder prepared by means of extracting banana skin polysaccharides for dairy cow |
| CN106804906A (en) * | 2016-12-21 | 2017-06-09 | 安徽强农牧业有限公司 | A kind of milk cow is with edible fungus fermented mulberry leaf composite chelate calcium powder |
| CN107669552B (en) * | 2017-10-31 | 2019-08-16 | 思拓科（上海）生物科技股份有限公司 | Organic baby's shampoo |
| CN109123062A (en) * | 2018-06-22 | 2019-01-04 | 青岛海洋生物医药研究院股份有限公司 | A kind of preparation method and application of peptide calcium chelate |
| CN111467377A (en) * | 2019-11-26 | 2020-07-31 | 天津科技大学 | Method for processing peptide calcium by using loach by-product |
| CN113317498B (en) * | 2021-06-08 | 2022-07-05 | 烟台金利昌食品有限公司 | Bone joint functional food and processing method thereof |
| CN114747715A (en) * | 2022-05-07 | 2022-07-15 | 深圳市豆柴宠物用品有限公司 | Natural antioxidant for pet food and preparation method thereof |
| CN118272478A (en) * | 2024-06-03 | 2024-07-02 | 青岛益和兴食品有限公司 | Method for preparing fishbone polypeptide by enzymolysis of complex enzyme and polypeptide preparation prepared by method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101928744A (en) * | 2010-04-27 | 2010-12-29 | 兰州城市学院 | Process for extracting active collagen peptide from salmon trout waste |
| CN101061827B (en) * | 2006-04-30 | 2012-01-25 | 中国食品发酵工业研究院 | Industry method of producing fish collagen peptide from fish skin and bone by an enzyme method |
| CN102406176A (en) * | 2011-10-08 | 2012-04-11 | 山东荣信水产食品集团股份有限公司 | Small molecule polypeptide Ca-chelate of fishbone and preparation method |
-
2013
- 2013-08-27 CN CN201310382788.2A patent/CN103404915B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101061827B (en) * | 2006-04-30 | 2012-01-25 | 中国食品发酵工业研究院 | Industry method of producing fish collagen peptide from fish skin and bone by an enzyme method |
| CN101928744A (en) * | 2010-04-27 | 2010-12-29 | 兰州城市学院 | Process for extracting active collagen peptide from salmon trout waste |
| CN102406176A (en) * | 2011-10-08 | 2012-04-11 | 山东荣信水产食品集团股份有限公司 | Small molecule polypeptide Ca-chelate of fishbone and preparation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103404915A (en) | 2013-11-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103404915B (en) | Complex chelated fish skin bone protein polypeptide calcium powder and preparation method thereof | |
| CN102871121B (en) | Preparation method of oceanic ossein peptide calcium chelate biological calcium supplement | |
| CN101731666B (en) | Fishbone bioactive polypeptide calcium powder and preparation method | |
| CN102406176A (en) | Small molecule polypeptide Ca-chelate of fishbone and preparation method | |
| CN106551391B (en) | A kind of pilose antler deep working method | |
| CN104095243B (en) | Preparation technology for enzymolysis bioactive peptide nanometer selenium | |
| CN102086464B (en) | Method for preparing chitin | |
| CN108796017A (en) | Ox bone peptide and its enzymatic extraction method | |
| CN103088097A (en) | Preparation method of matreel active peptide | |
| EP3071053B1 (en) | Prebiotic composition | |
| CN104012935B (en) | Processing method of fish sauce | |
| CN108402471A (en) | A kind of chelated calcium preparation method of livestock and poultry bone collagen polypeptide | |
| Guan et al. | Simulated digestion and in vitro fermentation of a polysaccharide from lotus (Nelumbo nucifera Gaertn.) root residue by the human gut microbiota | |
| CN105230783A (en) | Fishbone fermented milk and preparation method thereof | |
| CN105029429A (en) | Food additive enriched by gamma-aminobutyric acid and preparation method for food additive | |
| CN102823804A (en) | Production method of corn milk beverage using six-enzyme method | |
| CN109123036A (en) | Soybean-marrow peptide composition and application | |
| CN107788126A (en) | A kind of processing method for chelating calcium protein powder | |
| CN117137128A (en) | Noni enzyme juice and preparation method thereof | |
| CN105394381A (en) | Calcium supplement type digestion promoting piglet feed and preparation method thereof | |
| CN105996057A (en) | Fruit and egg white peptide tablets and preparing method thereof | |
| CN110521931A (en) | A kind of preparation method of black fungus fine dried noodle raw material, vermicelli and vermicelli | |
| CN101449737A (en) | Preparation method of scallop pure skirt amino acid nutrient powder | |
| CN102864197B (en) | Method for producing low-molecular weight collagen peptide by utilizing leftovers left by processing prawns | |
| CN105614855A (en) | Small peptide nutrition agent and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |