CN110623146A - Complex enzyme technology capable of improving energy utilization efficiency of livestock and poultry feed and application thereof - Google Patents

Complex enzyme technology capable of improving energy utilization efficiency of livestock and poultry feed and application thereof Download PDF

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CN110623146A
CN110623146A CN201911090124.2A CN201911090124A CN110623146A CN 110623146 A CN110623146 A CN 110623146A CN 201911090124 A CN201911090124 A CN 201911090124A CN 110623146 A CN110623146 A CN 110623146A
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enzyme
activity
beta
complex enzyme
daily ration
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宋全芳
余璐璐
李阳
严峰
彭翔
张广民
蔡辉益
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BEIJING CHALLENGE AGRICULTURE TECHNOLOGY Co Ltd
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BEIJING CHALLENGE AGRICULTURE TECHNOLOGY Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry

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  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

A complex enzyme capable of improving the energy utilization efficiency of livestock and poultry feed and an application thereof, aiming at improving the energy utilization efficiency of livestock and poultry daily ration. The compound enzyme comprises liquid fermentation enzyme, solid fermentation compound enzyme and a carrier, and the compound enzyme mainly comprises beta-mannase, and also comprises xylanase, beta-glucanase, pectinase and cellulase. In the complex enzyme, the activity of beta-mannase is 4000-. The complex enzyme is applied to low-energy daily ration, and can keep the normal requirement of animal organism on energy. The product of the invention has the advantages of green, environmental protection, no resistance and the like, and has higher popularization and application values.

Description

Complex enzyme technology capable of improving energy utilization efficiency of livestock and poultry feed and application thereof
Technical Field
The invention belongs to the field of feed additives, and particularly relates to a composite non-starch polysaccharide enzyme which is applied to low-energy daily ration and can effectively improve the energy utilization efficiency of feed and application thereof.
Background
With the continuous and high-speed development of the animal husbandry in China in recent years, the condition that main feed raw materials depend on import for a long time seriously influences the stability and development of the feed industry and the breeding industry in China. China has a large amount of grain processing byproducts (cotton seed meal, rapeseed meal, DDGS and the like), and the development and utilization of the non-grain resources are important ways for guaranteeing the continuous and healthy development of the animal husbandry in China. However, these byproducts often contain high-level non-starch polysaccharides (NSP) which exist as main anti-nutritional factors in the feed, so that the wide use of the byproducts in the breeding industry is limited, and the digestion and absorption of nutrients by livestock and poultry are also hindered.
Non-starch polysaccharides (NSP) are a generic term for all polysaccharide carbohydrates in plant tissues, except starch, lignin and small amounts of oligosaccharides, and mainly include cellulose, β -glucan, arabinoxylan, mannan, pectin and the like. The beta-mannan is hemicellulose which is widely distributed in feed raw materials such as corn, bean pulp, sesame pulp, coconut pulp, palm pulp and the like and is harmful to the digestion and absorption of animal nutrient substances, wherein the water-soluble beta-mannan is the hemicellulose with anti-nutrition, the anti-nutrition effect of the beta-mannan is far higher than that of xylan, glucan and the like, and the beta-mannan is mainly expressed as follows: increasing the viscosity of livestock and poultry intestinal chyme, so that the movement of intestinal contents is difficult; the interaction between digestive enzyme and substrate is blocked, the absorption of nutrients is physically blocked, and the water absorption is increased; the diffusion speed of the chyme to the surface layer of the mucous membrane is reduced, so that the absorption and utilization of nutrient substances are greatly influenced; in addition, β -mannan is a pathogen-associated molecular structure that is recognized by pattern recognition receptors in the gut to induce unwanted immune responses.
Most of the complex enzymes in the current market are prepared by combining single enzymes fermented in liquid state. Liquid fermentation is a domestic mainstream fermentation mode, and has the advantages of high production efficiency, sufficient sterilization and the like, but the complex enzyme compounded by liquid fermentation is difficult to reach a natural enzyme combination system generated by microorganisms in a wild state. The enzymes produced by solid state fermentation are rich in species, are adapted to the complexity of plant tissues, are more suitable for feeds, and destroy natural plant cell walls with complex chemical structures under the action of a complex enzyme system. At present, the research on the combined use of the solid fermentation enzyme and the liquid fermentation enzyme is less in China, and the research shows that the addition of the liquid fermentation single enzyme in the solid fermentation enzyme has obvious effects on daily gain and feed intake of nursery pigs, and the growth performance and the weight gain cost are superior to those of the liquid fermentation combined compound enzyme.
Therefore, the inventor utilizes a monogastric animal bionic digestion system and a broiler chicken feeding test to carry out combined effect research on complex enzyme mainly comprising beta-mannase and solid fermentation complex enzyme in vitro and in vivo and optimizes the proportional composition of the complex enzyme. The invention can obviously improve the energy utilization efficiency of livestock and poultry, reduce the production cost of livestock and poultry and improve the production performance of livestock and poultry.
Disclosure of Invention
Based on the shortage of energy feed raw materials in the current market, the invention aims to provide the feed additive which is applied to the daily ration of livestock and poultry and is used for improving the feed energy utilization efficiency and the application thereof. The complex enzyme product can improve the livestock and poultry breeding environment and achieve the purpose of improving the production performance.
The compound enzyme is formed by combining liquid fermentation enzyme and solid fermentation enzyme, wherein the mass ratio of the liquid fermentation enzyme is 25-50%, the mass ratio of the solid fermentation enzyme is 20-35%, and the mass ratio of the corn starch carrier is 15-55%.
The complex enzyme is prepared by compounding liquid-state fermented beta-mannase, xylanase, beta-glucanase, cellulase and solid-state fermented complex enzyme in proportion, wherein the activity of the beta-mannase is 8000U/g for 4000-one-year-old xylanase, the activity of the xylanase is 2000-4000U/g for 400-one-year-old 800U/g, the activity of pectinase is 1000U/g for 200-one-year-old and the activity of the cellulase is 600-one-year-old 600U/g.
Preferably, the activity of beta-mannase in the complex enzyme is 5000U/g, the activity of xylanase is 3000U/g, the activity of beta-glucanase is 600U/g, the activity of pectinase is 600U/g, and the activity of cellulase is 400U/g.
In the complex enzyme, the activity of beta-mannase is 4000-.
The xylanase activity of the solid fermentation enzyme in the complex enzyme is 3000-9000U/g, the beta-glucanase activity is 500-1200U/g, the pectinase activity is 1800-2500U/g, the cellulase activity is 300-600U/g, preferably, the xylanase activity is 4000U/g, the beta-glucanase activity is 800U/g, the pectinase activity is 2400U/g, and the cellulase activity is 400U/g.
The beta-mannase, the xylanase and the beta-glucanase are prepared by fermenting saccharomycetes, aspergillus niger, aspergillus oryzae, trichoderma longibrachiatum or bacillus subtilis and adopting a liquid submerged fermentation process.
The solid-state fermentation complex enzyme is produced by fermenting saccharomycetes, aspergillus niger, aspergillus oryzae, trichoderma longibrachiatum or bacillus subtilis and is prepared by adopting a solid-state submerged fermentation process.
The compound enzyme is composed of a plurality of single enzymes and solid state fermentation compound enzyme according to the optimal proportion; meanwhile, compared with the pure solid state fermentation enzyme and the pure liquid state fermentation enzyme, the compound enzyme has more excellent effect; the compound enzyme combination is finally determined by a large number of bionic digestion tests by the inventor and has a crucial influence on the effect of the invention.
The complex enzyme can be applied to the feeding process of broiler chickens, and more preferably, is applied to a low-energy daily ration formula of broiler chickens.
In a preferred embodiment of the invention, the final addition amount of the complex enzyme is 100-300 g/ton of complete feed.
The complex enzyme disclosed by the invention is applied to low-energy daily ration of broiler chickens, the feed cost can be obviously reduced, the production performance of the broiler chickens can be improved, the in-vitro effect of the complex enzyme disclosed by the invention has a remarkable effect when being applied to the low-energy daily ration, and the complex enzyme has a strong economic application benefit and a market popularization value.
Detailed Description
Example 1
The composite non-starch polysaccharide enzyme capable of improving the energy utilization efficiency of the livestock and poultry feed is prepared by compounding liquid-state fermented beta-mannase, xylanase, beta-glucanase, cellulase and solid-state fermented composite enzyme in proportion, wherein the activity of the beta-mannase is 5000U/g, the activity of the xylanase is 3000U/g, the activity of the beta-glucanase is 600U/g, the activity of the pectinase is 600U/g, and the activity of the cellulase is 400U/g.
Alternatively, the beta-mannanase activity is 5000U/g, the xylanase activity is 2000U/g, the beta-glucanase activity is 400U/g, and the cellulase activity is 300U/g.
Alternatively, the xylanase activity is 4000U/g, the beta-glucanase activity is 800U/g, the pectinase activity is 2400U/g, and the cellulase activity is 400U/g.
The beta-mannase, the xylanase and the beta-glucanase are prepared by fermenting saccharomycetes, aspergillus niger, aspergillus oryzae, trichoderma longibrachiatum or bacillus subtilis and adopting a liquid submerged fermentation process.
Example 2
In-vitro evaluation of influence of low-energy daily ration added with compound non-starch polysaccharide enzyme on reducing sugar release amount and in-vitro digestible energy of broiler feed
The procedures of bionic digestion of monogastric animals are as follows:
1. materials and methods
1.1 instruments and devices
A plant sample crusher or mortar; and (4) testing and screening: the aperture is 0.30mm (60 meshes); analytical balance: division value 0.0001 g; a pH meter: division value 0.01; a constant-temperature water bath kettle; a spectrophotometer; a dryer: anhydrous calcium chloride or allochroic silica gel is taken as a drying agent; a vortex oscillator; single stomach animal bionic digestive system (SDS-III)
1.2 reagents and materials and methods of treatment
Except specially noted, all reagents are analytically pure, and laboratory water should meet the specification of the third-level water in GB/T6682-.
Pepsin (Sigma P7000); amylase (Sigma a 3306); trypsin (Amresco 0785); chymotrypsin (Amresco 0164); hydrochloric acid (HCl); sodium chloride (NaCl); potassium chloride (KCl); anhydrous disodium hydrogen phosphate (Na)2HPO4) (ii) a Anhydrous sodium dihydrogen phosphate (NaH)2PO4) (ii) a Phosphoric acid (H)3PO4) (ii) a Sodium hydroxide (NaOH); potassium sorbate (C6H7KO 2); penicillin (160 ten thousand U); 3, 5-dinitrosalicylic acid (C)7H4N2O7) (ii) a Potassium sodium tartrate tetrahydrate (C)4H4KNaO6·4H2O); phenol (C)6H5OH); anhydrous sodium sulfite (Na)2SO3) (ii) a Glucose (Sigma G8270); a single use syringe; 0.22um filter membrane;
gastric buffer (pH 3.0): 2.59g of sodium chloride, 0.25g of potassium chloride, 6g of anhydrous sodium dihydrogen phosphate and 1g of potassium sorbate are weighed into a 500mL beaker, dissolved by adding 400mL of deionized water, and the pH of the solution is adjusted to 2.80 with 2mol/L hydrochloric acid (HCL) at 39 ℃. After cooling, the solution was transferred to a 500mL volumetric flask and was brought to volume with deionized water.
Small intestine buffer: 7.99g of anhydrous disodium hydrogen phosphate, 5.84g of anhydrous sodium dihydrogen phosphate, 1.265g of potassium sorbate and 60 million U of penicillin are weighed. Put into a 500mL beaker, dissolved by adding 180-200mL deionized water, and the pH of the solution is adjusted to 7.15 at 39 ℃ with 1mol/L phosphoric acid or 1mol/L sodium hydroxide. After cooling, the solution was transferred to a 250mL volumetric flask and was made to volume with deionized water.
Simulated gastric fluid (Pepsin Activity 737.5U/mL) 184.38ku of pepsin was weighed out to dissolve in 250mL of pH2.80 gastric buffer (pH calibrated at 39 ℃) according to a pepsin concentration of 737.5U/mL in the simulated gastric fluid, and stirred slowly until dissolved. The simulated gastric juice is not heated on a heating plate or overheated during preparation.
Simulated small intestine fluid (amylase activity 221.43U/mL, trypsin activity 69.10U/mL, chymotrypsin activity 8.68U/mL): according to the activity of the three digestive enzymes in simulated intestinal fluid, 41.41KU of amylase, 12.82KU of trypsin and 1.62KU of chymotrypsin are respectively weighed and dissolved in 17mL deionized water, and slowly stirred until dissolved. The preparation method comprises heating on a heating plate or overheating during preparation.
Glucose solution (concentration C (C)6H12O6) 10.0 mg/mL): 1.000g of anhydrous glucose is weighed, added with deionized water for dissolution, and the volume is adjusted to 100 mL.
Preparation of DNS color developing liquid
Sodium hydroxide solution (concentration C (NaOH)200g/L) 20.0g of NaHOH was weighed out, dissolved in deionized water, and the volume was adjusted to 100 mL.
3.15g of 3, 5-dinitrosalicylic acid is weighed, 500mL of water is added, the mixture is stirred for 5s, the mixture is bathed to 45 ℃, then 100mL of NaOH solution is gradually added while stirring is carried out continuously until the solution is clear and transparent (the temperature of the solution does not exceed 48 ℃ during the addition of NaOH). And then 91.0g of potassium sodium tartrate tetrahydrate, 2.50g of phenol and 2.50g of anhydrous sodium sulfite are gradually added, the water bath heating at the temperature of 45 ℃ is continued, 300mL of water is added simultaneously, the stirring is continuously carried out until the added substances are completely dissolved, the heating is stopped, the solution is cooled to the room temperature, and the volume is determined to be 1000mL by using water. Storing in brown bottle, keeping away from light, and standing at room temperature for 7 days for use with effective period of 6 months.
1.3 sample preparation
1.3.1 sample Collection
Sampling is performed according to GB/T14699.1.
1.3.2 sample treatment
The sampled sample is divided into about 200g by quartering method, and crushed by a plant crusher or a mortar to pass through a test sieve (60 meshes) with the aperture of 0.30mm, and sealed in a sample bag to be stored in a sealed manner to be used as a sample.
1.4 measurement procedure
1.4.1 preparation and Loading
1.4.1.1 the gastric buffer solution, the buffer solution at the front section of small intestine and the buffer solution at the rear section of small intestine are replaced by 1000mL deionized water and put into the corresponding positions of the bionic digestive system of monogastric animals, and the pipeline of the system is connected with the buffer solution bottle.
1.4.1.2 in the control software, the preheating time of the vertical digestion module of the bionic digestion system of monogastric animals is set to 60 min. And after the parameters of all digestion stages are input, running the simulated digestion process.
1.4.1.3 during the warming-up of the bionic digestive system of monogastric animals, the following loading work was performed.
1.4.1.4 cleaning the special glass simulated digestion tube and drying.
1.4.1.5A feed sample (to the nearest 0.0002 g) of 0.25-0.80g was weighed into a specially made glass simulated digestion tube. The dry matter content of the samples was determined simultaneously.
1.4.2 simulating gastrointestinal digestion in chickens
1.4.2.1 gastric simulated digestion
1.4.2.1.1 to the digestive tube was added 10mL of simulated gastric fluid.
1.4.2.1.2 the 10 simulated digesters were mounted on a vertical special module rack of a monogastric bionic digestive system.
1.4.2.1.3 the vertical special module is arranged in the bionic digestive system of monogastric animals, and the pipeline is connected according to the principle of simulating the water inlet at the lower end and the water outlet at the upper end of the digestive apparatus. Each group of 5 simulated digesters is connected in series. The digestive juice liquid adding pipe is connected with the system in sequence through a quick connector, and a plug of the motor is connected with a power supply.
1.4.2.1.4 in the control software of the bionic digestion system of monogastric animals, the parameters of the simulated digestion in the gastric stage are as follows: the temperature is 39 ℃, the rotation speed of a peristaltic pump is 180rpm/min, and the digestion time is 4 h. Other control parameters were run according to the instrument instructions.
1.4.2.2 simulated digestion of small intestine
1.4.2.2.1 at the end of the gastric simulated digestion, 6mL of small intestine buffer was automatically injected into the digestion tube by SDS-III peristaltic pump # 3, and then 1.6mL of simulated small intestine fluid was automatically injected into the simulated digestion tube.
1.4.2.2.2 in the control software of the bionic digestion system of monogastric animals, the parameters of the simulated digestion at the small intestine stage are as follows: the temperature is 39 ℃, the rotation speed of a peristaltic pump is 180rpm/min, and the digestion time of the small intestine is 16 h. Other control parameters were run according to the instrument instructions.
1.4.3 treatment of digestion residues
1.4.3.1 after digestion, transfer the digestive juice in the glass digestive tube to a 200mL clean volumetric flask without loss, then use deionized water to fix the volume, seal with a sealing film, shake up for standby (enzyme blank group does not need to be transferred to the volumetric flask, and filter membrane filtration is directly carried out).
1.4.3.2 take 30mL of digest from the vial, aspirate with a disposable syringe, filter through a 0.22um filter and get the filtrate ready for use.
1.4.4 preparation of glucose Standard Curve
1.4.4.1 sucking 4mL of deionized water into a 25mL graduated test tube, adding 5mL of DNS color developing solution, boiling in a water bath for 5min, cooling tap water to room temperature, and diluting deionized water to 25mL (directly adding 16mL of deionized water) to prepare a standard blank sample.
1.4.4.2 respectively sucking 10mg/mL glucose solution 1.00mL, 2.00mL, 3.00mL, 4.00mL, 5.00mL, 6.00mL and 7.00mL, respectively diluting with deionized water to 100mL, and preparing into glucose standard solution with concentration of 0.10 mg/mL-0.70 mg/mL.
1.4.4.3 sucking 2mL (2 parallel) of glucose standard solution with above concentration, adding into test tube, adding 2.0mL deionized water, mixing, adding 5.0mL DNS reagent, and mixing. Boiling in water bath for 5min, cooling tap water to room temperature, adding 16mL deionized water, and shaking up. The standard blank was zeroed and the absorbance OD measured at 540 nm.
1.4.4.4 Standard curves were plotted with glucose concentration on the Y-axis and absorbance OD on the X-axis. (the standard curve needs to be drawn again for each newly configured DNS color developing solution).
1.4.5 measurement of reducing sugar content
1.4.5.1A volume of 5mL of the digestion solution was added to the tube, and then 5mL of deionized water was added to dilute the solution by 2-fold.
1.4.5.2 adding 2mL diluted digestive juice into 25mL graduated test tube, adding 2mL deionized water, shaking, mixing, adding 5mL DNS color developing solution, mixing, and boiling water bath for 5 min. The tap water was cooled to room temperature, 16mL of deionized water was added, and the mixture was mixed well. The OD was measured in a spectrophotometer at 540nm (note that the OD should be between 0.1 and 0.5).
1.4.5.3 calculating reducing sugar content by substituting the measured OD value and dilution multiple into a glucose standard curve formula.
1.5 calculation of results
1.5.1 calculation formula
Total digestible carbohydrate (mg/g DM) ═ afxodMeasurement of+b)×2×200-(a×ODBlank space+b)×17.6)/(w×DM)
In the formula: a is a regression coefficient of a standard curve;
b is a standard curve regression constant;
ODmeasurement ofDetermining the absorbance value of the tube for each replicate;
ODblank spaceThe absorbance value of a blank tube of digestive enzyme is obtained;
w is the weight of each replicate tube feed sample;
DM is the dry matter content of the feed sample.
2. Test daily ration
Before the test is started, the used feed raw materials are collected, the conventional nutrient content of the feed raw materials is analyzed, the feed formula is prepared according to the actual detection result, the content of other nutrient components of each raw material refers to 'Chinese feed components and nutrient value table 2017', and the nutrient requirement refers to the feeding standard of NRC (1994) broiler chickens. Wherein the first daily ration is normal daily ration metabolic energy: 3000kcal/kg, and the second daily ration is low-energy daily ration metabolic energy: 2950kcal/kg, and the third daily ration is the metabolism energy of the low-energy daily ration: 2900kcal/kg, the group design is that the group I is a group without compound enzyme in the daily ration I, the group II is 0.15g/kg of compound enzyme in the daily ration II, the group III is 0.15g/kg of compound enzyme in the daily ration III, the group IV is 0.3g/kg of compound enzyme in the daily ration III, each group has 5 repetitions, each repetition has 1 digestive tract, and the test design is shown in the table 1:
the complex enzyme is prepared by fermenting yeast, aspergillus niger and the like by liquid or solid, and the production process is a conventional process and is not described in detail herein. The complex enzyme comprises beta-mannase with the activity of 5000U/g, xylanase with the activity of 3000U/g, beta-glucanase with the activity of 600U/g, pectinase with the activity of 600U/g and cellulase with the activity of 400U/g.
TABLE 1 design of the experiments
Group of Test daily ration
Group one Normal daily ration ME: 3000kcal/kg
Group two Low-energy daily ration ME: 2950kcal/kg +0.15g/kg complex enzyme
Group III Low-energy daily ration ME: 2900kcal/kg +0.15g/kg complex enzyme
Group IV Low-energy daily ration ME: 2900kcal/kg +0.30g/kg complex enzyme
The data analysis is carried out by adopting SPSS17.0 software ANOVO module single factor, and the P value is less than 0.05, which indicates that the difference is obvious. The test results are shown in table 2:
as can be seen from Table 2, compared with normal daily ration, the addition of 0.15g/kg of complex enzyme into daily ration with the metabolic energy ME of 2950kcal/kg has no obvious difference on the release amount of reducing sugar (P is more than 0.05); compared with normal daily ration, the addition of 0.15g/kg of complex enzyme in the daily ration with the metabolic energy ME of 2900kcal/kg obviously reduces the release amount of reducing sugar, and has obvious difference (P is less than 0.05); however, compared with the normal daily ration, the release amount of reducing sugar can reach the normal daily ration level (P is more than 0.05) by adding 0.30g/kg of complex enzyme in the daily ration with the metabolic energy ME of 2900 kcal/kg.
TABLE 2 Effect of adding Complex NSP enzymes to Low energy diets on reducing sugar Release amount
Group of Release amount of reducing sugar (mg/g)
Group one 577.36±3.35a
Group two 575.43±2.87a
Group III 568.11±3.26b
Group IV 579.68±4.37a
The difference between the different letters is significant (P < 0.05).
Example 3 Effect of adding Compound non-Amylase to Low energy diets on broiler Productivity
1. Test animal
In order to study the influence of adding compound NSP enzyme in low-energy daily ration on the production performance of broilers, 648 broilers of 1 day old were selected, and were randomly divided into 3 treatments according to male and female weights, each treatment was repeated for 12 times, each treatment was repeated for 18 chickens, the test period was 42 days, and feeding was performed in two stages.
2. Test daily ration
Before the test is started, the used feed raw materials are collected, the conventional nutrient content of the feed raw materials is analyzed, the feed formula is prepared according to the actual detection result, the content of other nutrient components of each raw material refers to 'Chinese feed components and nutrient value table 2017', and the nutrient requirement refers to the feeding standard of NRC (1994) broiler chickens. Wherein the first daily ration is normal daily ration metabolic energy: 3000kcal/kg, and the second daily ration is low-energy daily ration metabolic energy: 2950kcal/kg, and the third daily ration is the metabolism energy of the low-energy daily ration: 2900kcal/kg, wherein the group I is a group without compound enzyme in the daily ration I, the group II is a group with 0.15g/kg compound enzyme in the daily ration II, and the group III is a group with 0.15g/kg compound enzyme in the daily ration III; the experimental design is shown in table 3:
the complex enzyme is prepared by fermenting yeast, aspergillus niger and the like by liquid or solid, and the production process is a conventional process and is not described in detail herein. The complex enzyme comprises beta-mannase with the activity of 5000U/g, xylanase with the activity of 3000U/g, beta-glucanase with the activity of 600U/g, pectinase with the activity of 600U/g and cellulase with the activity of 400U/g.
TABLE 3 test design
Group of Test daily ration
Control group Normal daily ration ME: 3000kcal/kg
Processing a group Low-energy daily ration ME: 2950kcal/kg +0.15g/kg complex enzyme
Processing two groups Low-energy daily ration ME: 2900kcal/kg +0.15g/kg complex enzyme
3. Feeding management
The feeding test is carried out in a Beijing challenged biotechnology limited company following an animal test base, the test process is conventional feeding, free ingestion and drinking, and immunization and medication are operated according to immune program and medication guide of chicken farms.
The raising test is respectively carried out on an empty stomach in the morning when the test is started and ended, the daily feed intake is recorded in the test process, the average feed intake, the daily gain and the feed-meat ratio of the broilers in the whole stage are calculated after the test is ended, the data analysis is carried out by adopting SPSS17.0 software ANOVO module single factor, and the P value is less than 0.05, so that the difference is obvious. The test results are shown in table 4.
As can be seen from Table 4, the average daily gain and the average daily food consumption were not significantly different between the treatments (P > 0.05), but the average daily food consumption was reduced by 5.46% in the treated group compared to the control group; the feed-meat ratio is reduced by 1.78 percent (P is more than 0.05), the complex enzyme is added into the low-energy daily ration of the broiler chicken, the production performance of the broiler chicken is not obviously affected, and the feed-meat ratio of the broiler chicken tends to be reduced, so that the production cost is reduced.
TABLE 4 influence of Complex enzyme addition to Low energy diets on broiler performance
Group of Control group Processing a group Processing two groups
Average daily gain ADG (g) 50.51±2.73 50.79±2.05 51.34±1.97
Daily average feed intake ADFI (g) 85.42±3.14 83.76±4.56 86.75±3.64
Feed-meat ratio FCR 1.69±0.06 1.65±0.05 1.69±0.05
The research result of the invention shows that the production performance of livestock and poultry can be obviously improved by adding the complex enzyme in the low-energy daily ration, the normal nutrition level can be reached when the complex enzyme is applied in the low-energy daily ration, the energy utilization efficiency of the daily ration is obviously improved, the feed conversion ratio of broiler chickens is improved, and the production cost is saved.

Claims (5)

1. The composite non-starch polysaccharide enzyme capable of improving the energy utilization efficiency of livestock and poultry feed is characterized by comprising the following components in percentage by weight:
25% -50% of liquid fermentation single enzyme;
20-35% of solid-state fermentation complex enzyme;
15% -55% of a carrier;
the liquid fermentation enzyme comprises beta-mannase, xylanase, beta-glucanase and cellulase, wherein the activity of the beta-mannase is 4000-8000U/g, the activity of the xylanase is 1500-3500U/g, the activity of the beta-glucanase is 200-700U/g, the activity of the cellulase is 200-500U/g, and the activity of the solid fermentation complex enzyme is as follows: 3000-9000U/g xylanase, 1200-1200U/g beta-glucanase activity, 1800-2500U/g pectinase activity and 600-600U/g cellulase activity, wherein the carrier is corn starch.
2. The compound non-starch polysaccharide enzyme of claim 1, wherein the single-enzyme beta-mannase, xylanase and beta-glucanase are prepared by fermenting yeast, aspergillus niger, aspergillus oryzae, trichoderma longibrachiatum or bacillus subtilis through a liquid fermentation process; the solid-state fermentation complex enzyme is produced by fermenting saccharomycetes, aspergillus niger, aspergillus oryzae, trichoderma longibrachiatum or bacillus subtilis and is prepared by adopting a deep solid-state fermentation process.
3. The use of the compound non-starch polysaccharidase according to claims 1-2 in a broiler feeding process.
4. The use of claim 3, wherein the complex non-starch polysaccharidase is used in a low energy ration formulation for broiler chickens.
5. The application of the compound non-starch polysaccharide enzyme as claimed in claim 3, wherein the compound non-starch polysaccharide enzyme is added in an amount of 100-300 g/ton complete feed in the raising process of broiler chickens.
CN201911090124.2A 2019-11-08 2019-11-08 Complex enzyme technology capable of improving energy utilization efficiency of livestock and poultry feed and application thereof Pending CN110623146A (en)

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