CN113243453B - Biological preservative and application thereof in animal feed raw materials - Google Patents
Biological preservative and application thereof in animal feed raw materials Download PDFInfo
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- CN113243453B CN113243453B CN202110591660.1A CN202110591660A CN113243453B CN 113243453 B CN113243453 B CN 113243453B CN 202110591660 A CN202110591660 A CN 202110591660A CN 113243453 B CN113243453 B CN 113243453B
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- 239000003755 preservative agent Substances 0.000 title claims abstract description 57
- 230000002335 preservative effect Effects 0.000 title claims abstract description 57
- 241001465754 Metazoa Species 0.000 title claims abstract description 51
- 239000002994 raw material Substances 0.000 title abstract description 29
- 210000001835 viscera Anatomy 0.000 claims abstract description 51
- ZTHYODDOHIVTJV-UHFFFAOYSA-N Propyl gallate Chemical compound CCCOC(=O)C1=CC(O)=C(O)C(O)=C1 ZTHYODDOHIVTJV-UHFFFAOYSA-N 0.000 claims abstract description 36
- 108091005804 Peptidases Proteins 0.000 claims abstract description 20
- 239000004365 Protease Substances 0.000 claims abstract description 20
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims abstract description 18
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 18
- 235000010241 potassium sorbate Nutrition 0.000 claims abstract description 18
- 239000004302 potassium sorbate Substances 0.000 claims abstract description 18
- 229940069338 potassium sorbate Drugs 0.000 claims abstract description 18
- 235000010388 propyl gallate Nutrition 0.000 claims abstract description 18
- 229940075579 propyl gallate Drugs 0.000 claims abstract description 18
- 239000000473 propyl gallate Substances 0.000 claims abstract description 18
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims abstract description 18
- 235000010234 sodium benzoate Nutrition 0.000 claims abstract description 18
- 239000004299 sodium benzoate Substances 0.000 claims abstract description 18
- HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 claims abstract description 18
- 229940001584 sodium metabisulfite Drugs 0.000 claims abstract description 18
- 235000010262 sodium metabisulphite Nutrition 0.000 claims abstract description 18
- 150000003839 salts Chemical class 0.000 claims abstract description 17
- 235000002639 sodium chloride Nutrition 0.000 claims abstract description 17
- 229960003885 sodium benzoate Drugs 0.000 claims abstract description 8
- 235000013339 cereals Nutrition 0.000 claims abstract description 6
- 102000004190 Enzymes Human genes 0.000 claims description 21
- 108090000790 Enzymes Proteins 0.000 claims description 21
- 239000000463 material Substances 0.000 claims description 14
- 230000009849 deactivation Effects 0.000 claims description 12
- 238000005303 weighing Methods 0.000 claims description 12
- 230000000415 inactivating effect Effects 0.000 claims description 6
- 235000013372 meat Nutrition 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 241000287828 Gallus gallus Species 0.000 abstract description 42
- 239000000126 substance Substances 0.000 abstract description 8
- 238000003860 storage Methods 0.000 abstract description 6
- 230000006866 deterioration Effects 0.000 abstract description 5
- 244000005700 microbiome Species 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 239000002699 waste material Substances 0.000 abstract description 4
- 230000036039 immunity Effects 0.000 abstract description 3
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- 235000015097 nutrients Nutrition 0.000 abstract description 3
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- 231100000331 toxic Toxicity 0.000 abstract description 3
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- 239000003674 animal food additive Substances 0.000 abstract description 2
- 230000002035 prolonged effect Effects 0.000 abstract description 2
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- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 235000013330 chicken meat Nutrition 0.000 description 41
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 20
- 229910052757 nitrogen Inorganic materials 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 9
- 230000008859 change Effects 0.000 description 8
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- 235000013405 beer Nutrition 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 241000228245 Aspergillus niger Species 0.000 description 5
- 240000006439 Aspergillus oryzae Species 0.000 description 5
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 5
- 244000063299 Bacillus subtilis Species 0.000 description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 description 5
- 241000223262 Trichoderma longibrachiatum Species 0.000 description 5
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- 210000004185 liver Anatomy 0.000 description 4
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 241001148470 aerobic bacillus Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
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- 210000004798 organs belonging to the digestive system Anatomy 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
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- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 235000006439 Lemna minor Nutrition 0.000 description 1
- 244000242291 Lemna paucicostata Species 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000005536 corrosion prevention Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013364 duck meat Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
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- 230000000670 limiting effect Effects 0.000 description 1
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- 150000002772 monosaccharides Chemical class 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 235000013613 poultry product Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3499—Organic compounds containing oxygen with doubly-bound oxygen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3508—Organic compounds containing oxygen containing carboxyl groups
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3508—Organic compounds containing oxygen containing carboxyl groups
- A23L3/3517—Carboxylic acid esters
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/358—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nutrition Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
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- Fodder In General (AREA)
Abstract
The invention belongs to the technical field of feed additives, and particularly provides a biological preservative which comprises the following components in parts by weight: brewer's grains, sodium metabisulfite, sodium benzoate, potassium sorbate, propyl gallate and salt, and also provides the application of the biological preservative in animal viscera after protease enzymolysis. Effective components in animal viscera are converted into nutrient substances which are easy to be absorbed by animals through the hydrolysis of protease, so that the visceral function is fully exerted, and the waste of resources is avoided; the biological preservative is added into the product after the viscus enzymolysis, so that the safety is high, toxic and harmful substances are not contained, the growth of microorganisms can be obviously inhibited, the mildewing and deterioration are slowed down, the storage time of the animal viscus after the viscus enzymolysis is effectively prolonged, the raw material of the animal feed added with the biological preservative is added into the feed, the problem of deep development of an edible chicken slaughter house is solved, the resources are saved, the production performance and the immunity of the raised animals are improved, and the feed has good economic benefit.
Description
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to a biological preservative and application thereof in animal feed raw materials.
Background
As a world poultry industry big country, the poultry breeding history is long and the industry quantity is high, from the view of poultry product structure, the traditional poultry meat mainly takes chicken, and the proportion of duck meat output in the total poultry meat output is relatively small. With the rapid development of the edible chicken breeding industry, the yield of chicken viscera is increased year by year, but the feed cost is increased due to the shortage of feed raw materials in the world and the rapid increase of the prices of common feed raw materials such as soybean meal and the like, and the chicken viscera can be used as animal raw materials to be added into the feed in a small amount, so that the problem of deep development of an edible chicken slaughter house is solved. The chicken viscera is used as a feed raw material for domestic pet cats and dogs and is widely used, but most feed factories have very simple treatment modes for the chicken viscera, and the chicken viscera can be used as feed only by cleaning, crushing and drying; secondly, the internal organs of the chickens contain a large amount of non-digestible substances and useless substances, and after the internal organs of the chickens are eaten by animals, the burden of digestive organs is increased, and the diseases of the digestive organs of the animals are caused for a long time; finally, the effective components in the internal organs of the chickens cannot play a role, and particularly, the internal organs of the chickens contain immune protein, amino acid and the like, so that the functions cannot be fully played, and the waste of resources is caused.
Disclosure of Invention
The invention aims to solve the problems that the treatment mode is simple, the animal internal organs are easy to mildew and deteriorate and the effective components cannot play the role when the animal internal organs are used as feed raw materials in the prior art.
To this end, the present invention provides a biological preservative comprising: brewer's grains, sodium metabisulfite, sodium benzoate, potassium sorbate, propyl gallate and salt. Residual alcohol and organic acid in the brewer's grains can inhibit the growth of microorganisms and improve the preservative efficacy, and on the other hand, the brewer's grains contain rich dietary fibers, proteins, monosaccharides, amino acids and trace elements and can improve the nutritional value of the feed.
Specifically, the biological preservative comprises: 10-15 parts of brewer's grain, 1-2 parts of sodium metabisulfite, 0.5-0.1 part of sodium benzoate, 1-3 parts of potassium sorbate, 1-3 parts of propyl gallate and 3-5 parts of salt.
The invention also provides an animal feed raw material added with the biological preservative, which comprises the following components: animal viscera, protease and the biological preservative of any one of the above.
Specifically, the protease includes one or more proteases expressed by any one of Aspergillus niger, aspergillus oryzae, bacillus subtilis and Trichoderma longibrachiatum.
Specifically, the amount of the protease added is 300 to 1000U/g.
Specifically, the animal viscera include heart, liver, lung, kidney and intestine of slaughtered chicken.
The invention also provides a preparation method of the animal feed raw material added with the biological preservative, which comprises the following steps:
(1) Weighing a proper amount of animal internal organs, mincing the animal internal organs by a meat mincer, adding protease for enzymolysis, and inactivating the enzyme at high temperature after the enzymolysis;
(2) Preparing the biological preservative: weighing brewer's grains, sodium metabisulfite, sodium benzoate, potassium sorbate, propyl gallate and salt, and mixing uniformly;
(3) And (3) adding the biological preservative prepared in the step (2) into the animal viscera after enzyme deactivation, uniformly stirring and storing to obtain the animal feed raw material added with the biological preservative.
Specifically, the enzymolysis time in the step (1) is 3-6h, and the enzymolysis temperature is 45-55 ℃.
Specifically, the enzyme deactivation temperature in the step (1) is 85 ℃, and the enzyme deactivation time is 30min.
Specifically, the addition amount of the biological preservative in the step (3) is 15%.
Specifically, the storage temperature in the step (3) is 30 ℃.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the animal feed raw material added with the biological preservative is hydrolyzed by protease, so that effective ingredients in animal viscera are converted into nutrient substances which are easy to be absorbed by animals, the visceral function is fully exerted, and the waste of resources is avoided; the biological preservative comprising the brewer's grains, the sodium metabisulfite, the sodium benzoate, the potassium sorbate, the propyl gallate and the salt provided by the application are added into the product after the viscus enzymolysis, so that the biological preservative is high in safety, free of toxic and harmful substances, low in addition, capable of remarkably inhibiting the growth of microorganisms, slowing down mildew deterioration and effectively prolonging the storage time of the viscus of the animal after the viscus enzymolysis; the animal feed raw material added with the biological preservative is added into the feed, so that the problem of deep development of an edible chicken slaughter house is solved, the resource is saved, the production performance and the immunity of the fed animals are improved, and the feed has good economic benefits.
Detailed Description
The technical solutions in the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Although representative embodiments of the present invention have been described in detail, it will be understood by those skilled in the art that various modifications and changes may be made thereto without departing from the scope of the invention. Therefore, the scope of the present invention should not be limited to the embodiments, but should be defined by the appended claims and equivalents thereof.
The invention provides a biological preservative, which comprises: brewer's grains, sodium metabisulfite, sodium benzoate, potassium sorbate, propyl gallate and salt.
Specifically, the biological preservative comprises the following components in parts by weight: 10-15 parts of brewer's grain, 1-2 parts of sodium metabisulfite, 0.5-0.1 part of sodium benzoate, 1-3 parts of potassium sorbate, 1-3 parts of propyl gallate and 3-5 parts of salt. The beer lees contains a certain amount of alcohol capable of inhibiting the growth of bacteria, and the beer lees also contains rich protein, amino acid and trace elements, thereby being beneficial to improving the nutritive value of feed raw materials; the sodium metabisulfite has the oxidation resistance, generates sulfurous acid after absorbing water, can remove peculiar smell, has strong reducibility, can consume oxygen in food tissues, inhibits the activity of aerobic bacteria and the activity of enzymes in microorganisms, and further achieves the function of corrosion prevention; the sodium benzoate has the effects of preventing deterioration and souring and prolonging the shelf life; the potassium sorbate can effectively inhibit the activity of mould, microzyme and aerobic bacteria, thereby effectively prolonging the preservation time and keeping the original flavor; propyl gallate has oxidation resistance, and can slow down oxidation process and prevent mildew and deterioration.
The invention also provides an animal feed raw material added with the biological preservative, which comprises the following components: animal viscera, protease and the biological antiseptic.
Further, the protease includes one or more proteases expressed by any one of Aspergillus niger, aspergillus oryzae, bacillus subtilis, and Trichoderma longibrachiatum. Preferably, the addition amount of the protease is 300-1000U/g.
In the present invention, U/g means the activity of a single enzyme contained in each gram of a biological agent for digesting the internal organs of an animal, and the unit of activity (U) of a protease is defined as: hydrolyzing casein for 1min at 40 deg.C and pH of 10.5 to generate 1 μ g tyrosine, which is 1 enzyme activity unit U.
The invention also provides a preparation method of the animal feed raw material added with the biological preservative, which comprises the following steps:
(1) Weighing a proper amount of animal internal organs, mincing the animal internal organs by a meat mincer, adding protease for enzymolysis, and inactivating the enzyme at high temperature after the enzymolysis;
specifically, the enzymolysis time is 3-6h, and the enzymolysis temperature is 45-55 ℃; the enzyme deactivation temperature is 85 deg.C, and the enzyme deactivation time is 30min.
(2) Preparing the biological preservative: weighing brewer's grains, sodium metabisulfite, sodium benzoate, potassium sorbate, propyl gallate and salt, and mixing uniformly;
(3) And (3) adding the biological preservative prepared in the step (2) into the animal viscera after enzyme deactivation, wherein the adding amount is preferably 15%, uniformly stirring, and storing at 30 ℃ to obtain the animal feed raw material added with the biological preservative.
The effect of the biological preservative of the present invention in animal feed materials is investigated by the following specific examples.
Example 1:
the embodiment provides a chicken viscera feed raw material added with a biological preservative.
(1) Weighing 100g of a mixture of heart, liver, lung, kidney and intestine of slaughtered chicken, mincing with a meat mincer, adding mixed protease expressed by Aspergillus niger, aspergillus oryzae, bacillus subtilis and Trichoderma longibrachiatum at an addition amount of 1000U/g for enzymolysis for 4h at 50 deg.C, and inactivating enzyme at 85 deg.C for 30min;
(2) Preparing the biological preservative: weighing 10 parts of brewer's grains, 2 parts of sodium metabisulfite, 0.5 part of sodium benzoate, 1 part of potassium sorbate, 3 parts of propyl gallate and 5 parts of salt, and uniformly mixing;
(3) Adding the biological preservative prepared in the step (2) into the chicken viscera after enzyme deactivation by 15 percent of adding amount, stirring uniformly, and storing at 30 ℃.
The appearance of the chicken viscera feed material and the change of the content of volatile basic nitrogen are observed, and the results are shown in tables 1 and 2.
Example 2:
the embodiment provides a chicken viscera feed raw material added with a biological preservative.
(1) Weighing 100g of a mixture of heart, liver, lung, kidney and intestine of slaughtered chicken, mincing by a meat mincer, adding mixed protease expressed by Aspergillus niger, aspergillus oryzae, bacillus subtilis and Trichoderma longibrachiatum in an addition amount of 1000U/g for enzymolysis for 4h at 50 ℃, and inactivating enzyme at 85 ℃ for 30min;
(2) Preparing a biological preservative: weighing 12 parts of brewer's grain, 1.5 parts of sodium metabisulfite, 0.8 part of sodium benzoate, 2 parts of potassium sorbate, 2 parts of propyl gallate and 4 parts of salt, and uniformly mixing;
(3) Adding the biological preservative prepared in the step (2) into the chicken viscera subjected to enzyme deactivation in an addition amount of 15%, uniformly stirring, and storing at 30 ℃.
The appearance of the chicken viscera feed material and the change of the content of volatile basic nitrogen are observed, and the results are shown in tables 1 and 2.
Example 3:
the embodiment provides a chicken viscera feed raw material added with a biological preservative.
(1) Weighing 100g of a mixture of heart, liver, lung, kidney and intestine of slaughtered chicken, mincing with a meat mincer, adding mixed protease expressed by Aspergillus niger, aspergillus oryzae, bacillus subtilis and Trichoderma longibrachiatum at an addition amount of 1000U/g for enzymolysis for 4h at 50 deg.C, and inactivating enzyme at 85 deg.C for 30min;
(2) Preparing the biological preservative: weighing 15 parts of brewer's grains, 1 part of sodium metabisulfite, 1 part of sodium benzoate, 3 parts of potassium sorbate, 1 part of propyl gallate and 3 parts of salt, and uniformly mixing;
(3) Adding the biological preservative prepared in the step (2) into the chicken viscera after enzyme deactivation by 15 percent of adding amount, stirring uniformly, and storing at 30 ℃.
The appearance of the chicken viscera feed material and the change of the content of volatile basic nitrogen are observed, and the results are shown in tables 1 and 2.
Example 4:
the same method as in example 1 was used to prepare a chicken viscera feed material supplemented with a biological preservative, except that the amount of protease added was 300U/g.
The appearance of the chicken viscera feed material and the change of the content of volatile basic nitrogen are observed, and the results are shown in tables 1 and 2.
Comparative example 1:
the same procedure as in example 1 was used to prepare a chicken viscera feed material, except that no biological preservative was added. The appearance of the chicken offal feed materials and the change in volatile basic nitrogen content were observed, and the results are shown in tables 1 and 2.
Comparative example 2:
the same method as in example 1 was selected to prepare chicken viscera feed raw materials, except that the biological preservative comprises the following components: 1 part of sodium metabisulfite, 1 part of sodium benzoate, 3 parts of potassium sorbate, 1 part of propyl gallate and 3 parts of salt.
The appearance of the chicken viscera feed material and the change of the content of volatile basic nitrogen are observed, and the results are shown in tables 1 and 2.
Comparative example 3:
the same method as in example 1 was selected to prepare chicken viscera feed raw materials, except that the biological preservative comprises the following components: 15 portions of beer lees.
The appearance of the chicken viscera feed material and the change of the content of volatile basic nitrogen are observed, and the results are shown in tables 1 and 2.
TABLE 1 appearance Change of Chicken viscera feed raw materials
| Group number | Day 3 | Day 5 | Day 7 | Day 14 |
| Example 1 | Light fishy smell and no odor | Light fishy smell and no odor | Light fishy smell and no odor | Bland fishy smell, bland odor |
| Example 2 | Light fishy smell and no odor | Light fishy smell and no odor | Light fishy smell and no odor | Bland fishy smell |
| Example 3 | Light fishy smell and no odor | Light fishy smell and no odor | Light fishy smell and no odor | Bland fishy smell, bland odor |
| Example 4 | Light fishy smell and no odor | Light fishy smell and no odor | Light fishy smell and no odor | Bland fishy smell, bland odor |
| Comparative example 1 | Light fishy smell and no odor | Bland fishy smell, bland odor | Bland fishy smell | Discolored and smelly |
| Comparative example 2 | Light fishy smell and no odor | Light fishy smell and no odor | Light fishy smell and no odor | Bland fishy smell, bland odor |
| Comparative example 3 | Light fishy smell and no odor | Light fishy smell and no odor | Light fishy smell and no odor | Bland fishy smell, bland odor |
TABLE 2 volatile basic Nitrogen content variation
The reduction in table 2 is the reduction of volatile basic nitrogen in the same treatment time compared with comparative example 1 without adding preservative, and it can be seen from the results of examples 1-4 and comparative example 1 shown in tables 1 and 2 that the appearance of the chicken viscera feed raw material added with biological preservative of the invention is obviously improved and the content of volatile basic nitrogen is obviously reduced compared with the chicken viscera without adding biological preservative, which indicates that the chicken viscera feed added with biological preservative of the invention effectively improves the product preservation quality, greatly increases the storage time and better improves the utilization rate of the chicken viscera as animal feed raw material. In addition, the results of the examples and the comparative examples 2 to 3 show that the beer lees, sodium metabisulfite, sodium benzoate, potassium sorbate, propyl gallate and salt have a synergistic effect, and the storage quality of the chicken viscera feed raw materials can be improved by compounding the beer lees, the sodium metabisulfite, the sodium benzoate, the potassium sorbate, the propyl gallate and the salt.
In conclusion, the animal feed raw material added with the biological preservative provided by the invention is hydrolyzed by protease to convert effective components in animal viscera into nutrient substances which are easy to be absorbed by animals, so that the viscera function is fully exerted, and the waste of resources is avoided; the preservative beer lees, sodium metabisulfite, sodium benzoate, potassium sorbate, propyl gallate and salt are added into the product after the viscus enzymolysis, so that the safety is high, toxic and harmful substances are not contained, the addition amount is low, the growth of microorganisms can be obviously inhibited, the mildew deterioration is slowed down, the storage time of the viscus of the animal after the viscus enzymolysis is effectively prolonged, the raw material of the animal feed added with the biological preservative is added into the feed, the problem of deep development of an edible chicken slaughterhouse is solved, the resource is saved, the production performance and the immunity of the raised animal are improved, and the method has good economic benefit.
The above examples are merely illustrative of the present invention and should not be construed as limiting the scope of the invention, which is intended to be covered by the claims and any design similar or equivalent to the scope of the invention.
Claims (5)
1. A biological preservative, comprising: 10-15 parts of brewer's grain, 1-2 parts of sodium metabisulfite, 0.5-0.1 part of sodium benzoate, 1-3 parts of potassium sorbate, 1-3 parts of propyl gallate and 3-5 parts of salt.
2. The use of a biological preservative according to claim 1 for improving the preservation quality of animal feed materials, comprising the steps of:
(1) Weighing a proper amount of animal internal organs, mincing the animal internal organs by a meat mincer, adding protease for enzymolysis, and inactivating the enzyme at high temperature after the enzymolysis;
(2) Preparing the biological preservative: weighing brewer's grains, sodium metabisulfite, sodium benzoate, potassium sorbate, propyl gallate and salt, and mixing uniformly;
(3) Adding the biological preservative prepared in the step (2) into the animal viscera after enzyme deactivation, stirring uniformly, and storing at 30 ℃.
3. The use of a biological preservative according to claim 2 for improving the preservation quality of animal feed materials, wherein: the enzymolysis time in the step (1) is 3-6h, and the enzymolysis temperature is 45-55 ℃.
4. The use of a biological preservative according to claim 2 for improving the preservation quality of animal feed materials, wherein: in the step (1), the enzyme deactivation temperature is 85 ℃, and the enzyme deactivation time is 30min.
5. The use of a biological preservative according to claim 2 for improving the preservation quality of animal feed materials, wherein: the addition amount of the biological preservative in the step (3) is 15%.
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| GB1495933A (en) * | 1974-12-17 | 1977-12-21 | Hypromel Prod Ltd | Offal processing |
| IE782318L (en) * | 1978-11-23 | 1980-05-23 | Michael Payne | Foodstuff preservative. |
| CN109043134A (en) * | 2018-08-21 | 2018-12-21 | 开原市嬴德肉禽有限责任公司 | The technique for preparing feedstuff using discarded internal organ and blood |
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