TW202133731A - Use of nutritional composition being prepared by adding a yeast into a soybean protein culture medium and an antrodia camphorata extract to perform fermentation - Google Patents

Abstract

This invention relates to a use of a nutritional composition. The nutritional composition is prepared by adding yeast into a soybean protein culture medium and an antrodia camphorata extract to perform fermentation, and the nutritional composition is used for reducing the feed conversion ratio and the body fat of perches and accelerating the growth of intestinal villi of the perches and the area increment of muscles. Therefore, the grown speeds of cultured fishes are increased, and the time to market is shortened.

Description

Use of nutritional composition

The present invention relates to a nutritional composition, especially the use of a nutritional composition.

As the global population increases and the area of arable land decreases significantly, the demand for food continues to rise. However, the large-scale production model consumes a certain amount of resources and causes environmental pressure. For this reason, increasing the production of a single area and increasing the added nutritional value are the trends of world food production.

However, due to overfishing and pollution of the sea area, traditional capture fisheries have led to a decline in catches year by year. At the same time, the catch of capture fisheries is estimated to have reached its limit. Therefore, in recent years, it has been the aquaculture industry that has made up for the lack of supply of fishery products (only traditional fisheries) to meet the demand for aquatic products in the global market.

However, aquaculture has always been one of China's important economic industries. In the past 20 years, the rapid development of aquaculture has brought abundant revenue to our country. At present, in addition to shrimps, aquaculture organisms in China also include a variety of economic fish species, such as grouper, mullet, milkfish, perch and sea mussel.

In the breeding process, in order to inhibit the germs of aquaculture organisms or accelerate their growth, it is still inevitable to use chemicals to control the breeding environment. However, the world's farming medicine regulations are becoming more stringent, and the types of chemicals available are gradually decreasing. In addition, chemical agents may also remain in the organism, and may endanger human health after consumption.

Due to the shortcomings of the above-mentioned chemical agents, it is now the most common practice to improve the composition of the feed formula. Feed originally refers to the material that can provide the necessary organic or inorganic nutrients to maintain the survival of livestock and the production of milk, meat, eggs, and fur.

However, today's feed system has evolved not only from the nutrients needed by various domestic animals, such as energy, protein, vitamins and minerals, but also from a uniform mixture of growth promoters and disease preventive agents.

The main protein source of Xi Aquafeed is fish meal. The average price per kilogram of fish meal is 60-65 yuan. However, due to the pollution of the marine environment, global climate change and the destruction of the ecological environment in recent years, the global fish meal raw material shortage has caused aquaculture industry. The cost of feed is still high. Therefore, many traditional aquatic feeds have replaced part of fish meal with lower-priced soybean meal to reduce the cost of traditional aquatic feeds.

Although soybean meal has a low cost per kilogram (about 15 yuan) and can be used as a substitute for fish meal to provide essential amino acids for the growth of aquatic animals, however, due to the imbalance of amino acids contained in soybeans, such as The lack of methionine can not completely replace fish meal to supply the nutrients needed for the growth of aquaculture animals.

In addition, because soybeans contain many anti-nutrient substances, for aquaculture animals, soybean meal will cause a problem of obstacles to nutrient absorption of aquaculture animals. Therefore, the ratio of soybean meal in Xi'an Aquatic Feed that can replace fish meal is limited, usually not exceeding 40% of the original fish meal amount, to avoid the problem of low feed conversion rate caused by soybean anti-nutrients.

Therefore, how to improve the feed formula to increase the growth rate of farmed fish, shorten the time to market, reduce the feed conversion rate (feed weight/fish weight gain), reduce feed costs, and reduce possible occurrences during breeding Risk is a direction worthy of research, and it has now become a topic of current research by those skilled in the art.

The main purpose of the present invention is to provide a use of a nutritional composition. The nutritional composition is obtained by adding soybean protein medium and Antrodia cinnamomea extract to yeast for fermentation, and the nutritional composition is used to reduce the feed conversion rate of sea bass and Body fat, and promote the growth of small intestine villi and muscle area of sea bass.

In order to achieve the above-mentioned purpose and effect, the present invention discloses a use of a nutritional composition for villus hyperplasia, wherein the nutritional composition is formed by a soybean culture medium, an Antrodia cinnamomea extract through a fermentation process with a brewer's yeast strain. , The nutritional composition is used for sea bass and the nutritional composition further comprises a small molecule peptide, the small molecule peptide is below 3kDa, and the preparation method of the nutritional composition comprises: taking a soybean protein and a protease And a cellulase are subjected to a hydrolysis process to form the soybean culture medium; and the soybean culture medium and the Antrodia camphorata extract are taken in a ratio of 8:2 to undergo a fermentation process with the brewer's yeast strain to form the nutrient composition.

The present invention provides an embodiment, the content of which is that the nutritional composition is used for villus hyperplasia, and the nutritional composition further includes an organic acid, a bacteriostat and superoxide dismutase active substances.

The present invention provides an embodiment, the content of which is the use of the nutritional composition for villus hyperplasia, wherein 1 gram of the nutritional composition is added per kilogram of feed and mixed and fed to sea bass.

In addition, the present invention discloses the use of a nutrient composition for reducing feed conversion of sea bass, wherein the nutrient composition is formed by a soybean culture medium, an Antrodia cinnamomea extract through a fermentation process with a brewer's yeast strain, and the The nutritional composition further includes a small molecule peptide, the small molecule peptide is below 3kDa, and the preparation method of the nutritional composition includes: taking a soybean protein and performing a hydrolysis process with a protease and a cellulase to form the Soybean culture medium; and taking the soybean culture medium and the Antrodia cinnamomea extract in a ratio of 8:2 to perform a fermentation process with the brewer's yeast strain to form the nutrient composition.

The present invention provides an embodiment, the content of which is the use of the nutritional composition for reducing feed conversion of sea bass, wherein 1 gram of the nutritional composition per kilogram of feed is mixed and fed to the sea bass.

In addition, the present invention discloses the use of a nutritional composition for reducing the body fat of sea bass, wherein the nutritional composition is formed by a soybean culture medium, an Antrodia cinnamomea extract through a fermentation process with a brewer's yeast strain, and the The nutritional composition further includes a small molecule peptide, the small molecule peptide is below 3kDa, and the preparation method of the nutritional composition includes: taking a soybean protein and performing a hydrolysis process with a protease and a cellulase to form the Soybean culture medium; and taking the soybean culture medium and the Antrodia cinnamomea extract in a ratio of 8:2 to perform a fermentation process with the brewer's yeast strain to form the nutrient composition.

The present invention provides an embodiment, the content of which is the use of the nutritional composition for reducing the body fat of sea bass, wherein 1 gram of the nutritional composition per kilogram of feed is mixed and fed to the sea bass.

In addition, the present invention discloses the use of a nutrient composition for increasing the muscle area of sea bass, wherein the nutrient composition is formed by a soybean culture medium, an Antrodia cinnamomea extract through a fermentation process of a brewer's yeast strain, and the The nutritional composition further includes a small molecule peptide, the small molecule peptide is below 3kDa, and the preparation method of the nutritional composition includes: taking a soybean protein and performing a hydrolysis process with a protease and a cellulase to form the Soybean culture medium; and taking the soybean culture medium and the Antrodia cinnamomea extract in a ratio of 8:2 to perform a fermentation process with the brewer's yeast strain to form the nutrient composition.

The present invention provides an embodiment, the content of which is the use of the nutritional composition for increasing the muscle area of sea bass, wherein 1 g of the nutritional composition per kilogram of feed is mixed and fed to the sea bass.

In order to enable your reviewer to have a further understanding and understanding of the features of the present invention and the effects achieved, I would like to provide examples and accompanying explanations. The description is as follows:

With the increase of the global population, the number of food needs continues to rise, and the large-scale production model consumes resources and causes environmental pressure. Therefore, in order to achieve the vision of sustainable production, how to adopt an efficient food production model has gradually been paid attention. In terms of economic animal husbandry, aquatic animals have a better "feed conversion ratio (FCR)" compared to livestock and poultry animals. Therefore, they are recognized by the High Level Panel of Experts on Food (HLPE), a think tank team of the United Nations Food and Agriculture Organization (FAO) Security and Nutrition believes that less resources can be invested in breeding (2014), and it is relatively friendly to the environment.

The feed conversion rate (FCR) is calculated by dividing the weight of feed consumed by the animal by the body weight of the animal, that is, the weight of feed that the animal needs to ingest per kilogram of body weight. Therefore, if the FCR is 3/1, it means that 3 kilograms of feed are needed to increase the weight of one kilogram of cultured organisms.

Therefore, how to improve the feed to reduce the feed conversion rate and reduce environmental pollution. It is a problem that people in the breeding field need to face.

In view of the traditional feeds, the production speed of farmed fish is too slow, the feed conversion rate is too high, and the feed cost is too high. Accordingly, the present invention proposes a use of the nutritional composition to solve the problems caused by the conventional technology.

The following will further explain the use of the nutritional composition of the present invention, its included characteristics, matched structure and preparation method:

Please refer to Figure 1, which is an experimental flow chart of an embodiment of the present invention. As shown in the figure, a method for preparing a nutritional composition of the present invention includes the following steps:

S1: Take soy protein and hydrolyze it with protease and cellulase to form a soy culture medium; and

S2: Take soybean culture medium and Antrodia cinnamomea extract in a ratio of 8:2 for fermentation process to form a nutrient composition.

As shown in step S1, take a soy protein (1 ton of dehulled soy flour) and perform a hydrolysis process with a cellulase (about 350 g) at 50°C for 1 hour, and then at 50°C for 3 hours The hydrolysis process is carried out with a protease (approximately 100 grams) under the conditions, and a soybean culture medium is formed after drying.

And following step S2, an Antrodia cinnamomea extract of the present invention contains a triterpenoid and a polysaccharide extract. And before step S2, it further includes the steps:

S11: Take Antrodia cinnamomea fruit bodies for an organic solution extraction process to obtain triterpenoids;

S12: Take Antrodia cinnamomea fruiting bodies for an aqueous extraction process to obtain polysaccharide extracts; and

S13: Mix triterpenoids and polysaccharide extracts to form Antrodia camphorata extracts.

As shown in step S11, take 1 ton of Antrodia cinnamomea fruiting bodies for an organic solution extraction process (soaking the Antrodia cinnamomea fruiting bodies with 95% ethanol for 30 days to rupture the cells), and after extraction, concentrate under reduced pressure and undergo 400 kg of powder was obtained by drying. After purification and analysis, the powder contained 4.25% triterpenoids.

。The chemical structure of the triterpenoid compound of the present invention is

.

As shown in step S12, take 1 ton of Antrodia cinnamomea fruiting bodies for an aqueous solution extraction process (place the Antrodia cinnamomea fruiting bodies in a hot reflux extraction device at 100°C for 24 hours), and cool after extraction, and then pass Centrifugation, purification, drying and other processes can obtain 400 powder. The extract is analyzed and contains 5% polysaccharide extract.

As shown in step S13, the triterpenoid compound and the polysaccharide extract are mixed to form the Antrodia camphorata extract.

Finally, step S2 is performed, and the soybean medium and the Antrodia cinnamomea extract are mixed in a ratio of 8:2. In addition, the brewer's yeast strain ( Saccharomyces cerevisiae ) will be used to perform the fermentation process to form the nutritional composition.

The culture process of the brewer’s yeast strain is to grow the bottle first to serve as the seed strain connected to the fermentation tank. It is yeast extract, malt extract, egg whites, glucose, etc.), connected to a 1ml glycerin preservation yeast tube, cultivated at 30°C for 24 hours, and then 100 ml of yeast liquid is continuously expanded to 10L liquid medium, the same Incubate at 30°C for 24 hours as a seed bottle for fermentation tank production. Then the main fermentation is carried out, and the seed bottle bacteria liquid is taken into the fermentation tank (the liquid medium for the main fermentation is the above-mentioned integrated medium powder, which is then dissolved by adding water. The production ratio of each ton of liquid medium is 20 kg of integrated medium powder and 1 ton of water for stirring and dissolving. Usually the fermentation volume is greater than 1 ton), the inoculation amount is 1%, the fixed pH value is 5, the aeration rate is 1 vvm, 120 rpm, and 30°C for 8 days to obtain the nutrient composition.

Wherein, the nutritional composition contains the small molecule peptide (which is extracted and fermented from the soybean protein, and the small molecule peptide is below 3kDa). Furthermore, the nutritional composition of the present invention is analyzed by fast gel filtration chromatograph for the content of polypeptides. After analysis, the nutritional composition of the present invention has a molecular weight of less than 2.5 kDa of the small molecule peptides, which account for The content is as high as 71.56% (as shown in Figure 2). In addition, the nutritional composition of the present invention is rich in a large amount of active substances such as small molecule peptides, organic acids, Bacteriocin, Antrodia camphorata extract, and superoxide dismutase (SOD) after analysis.

Next, a nutrient composition of the present invention is used for villus hyperplasia, wherein the nutrient composition is formed from the soybean culture medium, the Antrodia cinnamomea extract through the fermentation process of the brewer's yeast strain, and the nutrient composition is used For perch. Its use for reducing the feed conversion rate of seabass, promoting the growth of seabass fluff, reducing body fat of seabass, and increasing the muscle area of seabass is carried out by the following experimental examples, and the preparation methods are all the same.

Experimental example

Animal grouping and feeding methods

The nutritional composition of the present invention is used for sea bass (but not limited to this, and can also be cattle, sheep, chickens, pigs, etc.). In this experiment, the present invention uses California bass (Micropterus salmoides; scientific name largemouth bass) as the experimental animal, and divides California bass into two groups. The grouping and feeding methods are as follows:

(1) In the control group, 5,000 California sea bass were fed daily with a commercial feed made mainly of ground soybean protein (the feed amount was about 80-100 g/head), and the test period was 60-90 days (called Blank group).

(2) In the experimental group, 5,000 California sea bass were fed with the nutritional composition of the present invention (the amount of the nutritional composition was about 80-100 g/head), and the test period was 60-90 days (referred to as the Test group). ).

experimental method

Regardless of the control group or the experimental group, 30 California bass were sampled every 14 days for testing. After the California sea bass was sacrificed, small intestinal villi tissue, liver tissue and muscle tissue were obtained and fixed with 10% buffered formalin solution. After sampling, they were dehydrated by alcohol, buffered by xylene, and infiltrated with paraffin. Finally, it is embedded and stored in paraffin. After that, use a microtome to cut into an appropriate thickness (2~5μm) and adhere to a glass slide. According to hematoxylin and eosin, stain the sections of small intestinal villi tissue, liver tissue, and muscle tissue, and observe .

Experimental result

According to the above experimental method, please refer to Figure 3-6 for the experimental results. First of all, Figure 3 is a graph showing the experimental results of the feed conversion rate of an embodiment of the present invention. As shown in the figure, the feed conversion rate (the weight of feed the animal eats divided by the animal's body weight gain, FCR) at week 0 of the control group and the experimental group were 1.656 and 1.711, respectively. After 10 weeks of feeding, the FCR of the experimental group (which is the nutritional composition of the present invention) decreased to 1.27 compared to the control group (the FCR of which was 1.57), indicating that the nutritional composition of the present invention is used to reduce perch The feed conversion rate has better feed utilization efficiency for California sea bass.

Continuing, Fig. 4 is a graph showing the experimental results of the villi tissue of an embodiment of the present invention. As shown in the figure, after 10 weeks of feeding, compared with week 0 and compared with the control group, the length of the small intestine villi of the experimental group (which is the nutritional composition of the present invention) increased by an average of 8%. It is shown that the nutritional composition of the present invention is used to promote the growth of villi of sea bass.

Furthermore, Fig. 5 is a graph showing the experimental results of liver tissue according to an embodiment of the present invention. As shown in the figure, at week 0, the liver tissues of the control group and the experimental group were both vacuolated, indicating that the liver cells could not be metabolized due to excessive fat, and then accumulated in the liver cells to form fatty liver. And it makes the melanin macrophages involved in the immune response unable to infiltrate and attack bacteria. After 10 weeks of feeding, the liver tissue of the control group still showed cell vacuolation1. On the other hand, the experimental group (the nutrient composition of the present invention) can quickly repair liver cells to have intact cells2, reduce cell cavitation, and It can be observed that melanin macrophages 3 are present in the liver tissue. It is shown that the nutritional composition of the present invention is used to reduce the body fat of sea bass and restore the melanin macrophages 3 in the liver to enhance the immune effect.

Finally, Figure 6 is a graph showing the experimental results of muscle tissue according to an embodiment of the present invention. As shown in the figure, after 10 weeks of feeding, compared with week 0 and compared with the control group, the muscle area of the sea bass fed with the experimental group (the nutritional composition of the present invention) increased by 27% on average. It is shown that the nutritional composition of the present invention is used to increase the muscle area of sea bass.

The nutritional composition of the present invention is used for villus hyperplasia. It is different from the conventional feed. The nutritional composition of the present invention can effectively and quickly increase the growth of villi cells and enhance the absorption of feed nutrients by sea bass. Furthermore, it can be seen from the reduction of the FCR value that the nutritional composition of the present invention can greatly reduce the cost of feed and labor input. It can quickly enhance the immunity of sea bass and effectively resist bacterial or viral infections.

Still further, the small molecule peptide (derived from the soybean protein) in the nutritional composition of the present invention is compared with the relevant fish feed on the market to prove that the small molecule peptide of the present invention is effect. The method of grouping and rearing is as follows: Take 5000 California bass, feed about 80-100 g/head per day, and the test period is 60-90 days. And as shown in the following table 2-5, it is a commercially available fish feed (table 1: general commercial feed (group A), table 2: the small molecule peptide added to the nutritional composition of the present invention (from the soybean protein Related feed (group B), table 3: Yongxucheng feed (group C), and table 4: Dabang feed (group D).

The corresponding experimental results are shown in Figures 7A, 7B, and 8, which are the experimental results of the fluff length of the nutritional composition of the present invention and the commercially available fish feed, and the fluff length of the nutritional composition of the present invention and the commercially available fish feed The experimental bar graph and the experimental bar graph of the feed conversion rate of the small molecule peptide (extracted from the soybean protein) of the nutritional composition of the present invention and the commercially available fish feed. Table 1. Composition ratio of general commercial feed Weight (kg) Percentage of feed Alaska Fish Meal 418 41.8 flour 200 20 Cooked Soy Flour 250 25 Scallop powder 50 5 Salmon fish oil 50 5 Squid oil 10 1 Calcium Dihydrogen Phosphate 10 1 Organic minerals 1 0.1 vitamin 0.8 0.08 Salt 3 0.3 choline 3 0.3 Lecithin 2.7 0.27 Vitamin C 0.5 0.05 Vitamin E 1 0.1 1000 100 Table 2. Ratio of relevant feed ingredients added with nutrient composition Weight (kg) Percentage of feed Alaska Fish Meal 418 41.8 flour 200 20 Cooked Soy Flour 249 25 Small molecule peptides 1 0.1 Scallop powder 50 5 Salmon fish oil 50 5 Squid oil 10 1 Calcium Dihydrogen Phosphate 10 1 Organic minerals 1 0.1 vitamin 0.8 0.08 Salt 3 0.3 choline 3 0.3 Lecithin 2.7 0.27 Vitamin C 0.5 0.05 Vitamin E 1 0.1 1000 100 Table 3. Composition ratio of Yongxucheng feed Weight (kg) Percentage of feed Alaska Fish Meal 418 41.8 flour 200 20 Cooked Soy Flour 50 5 Yongxucheng soy protein 200 20 Scallop powder 50 5 Salmon fish oil 50 5 Squid oil 10 1 Calcium Dihydrogen Phosphate 10 1 Organic minerals 1 0.1 vitamin 0.8 0.08 Salt 3 0.3 choline 3 0.3 Lecithin 2.7 0.27 Vitamin C 0.5 0.05 Vitamin E 1 0.1 1000 100 Table 4. Composition ratio of Dabang feed Weight (kg) Percentage of feed Alaska Fish Meal 418 41.8 flour 200 20 Cooked Soy Flour 50 5 Dabang Fermented Soy Flour 200 20 Scallop powder 50 5 Salmon fish oil 50 5 Squid oil 10 1 Calcium Dihydrogen Phosphate 10 1 Organic minerals 1 0.1 vitamin 0.8 0.08 Salt 3 0.3 choline 3 0.3 Lecithin 2.7 0.27 Vitamin C 0.5 0.05 Vitamin E 1 0.1 1000 100

And as shown in Tables 1 to 4, the small molecule peptide (extracted from the soybean protein) of the nutritional composition of the present invention only replaces 1 kg. The experimental results are shown in Figures 7A-7B. After adding the small molecule peptide (derived from the soybean protein extract and fermented) to the nutritional composition of the present invention (group B), the length of its fluff is compared with the other three groups (group A-general commercial feed, C group-Yongxucheng feed, D group-Dabang feed) have increased a lot, showing that only a small amount of the small molecule peptide (extracted from the soybean protein) in the nutritional composition of the present invention needs to be added Let the length of the perch's fluff grow.

Also, referring to Figure 8, after adding the small molecule peptide (derived from the soybean protein) in the nutritional composition of the present invention (group B), the feed conversion rate of sea bass is higher than that of the other three groups ( Group A-general commercial feed, group C-Yongxucheng feed, group D-Dabang feed) is low, showing that only a small amount of the small molecule peptide (extracted from the soybean protein) needs to be added to the nutritional composition of the present invention Come) to further reduce the feed conversion rate of sea bass.

Therefore, the present invention is really novel, progressive, and available for industrial use. It should meet the patent application requirements of China's patent law. Undoubtedly, I filed an invention patent application in accordance with the law. I pray that the Bureau will grant the patent as soon as possible.

However, the above are only the preferred embodiments of the present invention, and are not used to limit the scope of implementation of the present invention. For example, the shapes, structures, features and spirits described in the scope of the patent application of the present invention are equally changed and modified. , Should be included in the scope of patent application of the present invention.

1: Cell vacuolation 2: intact cell 3: Melanin macrophages S1~S2: step flow

Figure 1: It is an experimental flowchart of an embodiment of the present invention;

Figure 2: It is the analysis result figure of the nutritional composition of the present invention;

Figure 3: It is a graph of the experimental results of the feed conversion rate of an embodiment of the present invention;

Figure 4: It is a graph of the experimental results of the villi tissue of an embodiment of the present invention;

Fig. 5: It is a graph of experimental results of liver tissue according to an embodiment of the present invention;

Figure 6: It is a graph of experimental results of muscle tissue in an embodiment of the present invention;

Figure 7A: It is a graph showing the experimental results of the fluff length of the nutritional composition of the present invention and commercially available fish feed;

Figure 7B: It is an experimental bar graph of the fluff length of the nutritional composition of the present invention and commercially available fish feed; and

Figure 8: It is an experimental bar graph of the feed conversion ratio between the nutritional composition of the present invention and the commercially available fish feed.

Claims (8)

A nutrient composition is used for villus hyperplasia, wherein the nutrient composition is formed by a soybean culture medium, an Antrodia cinnamomea extract through a fermentation process of a brewer's yeast strain, wherein the nutrient composition is used for sea bass and the The nutritional composition further includes a small molecule peptide, the small molecule peptide is below 3kDa, and the preparation method of the nutritional composition includes: Take a soybean protein and perform a hydrolysis process with a protease and a cellulase to form the soybean culture medium; and The soybean culture medium and the Antrodia cinnamomea extract are taken in a ratio of 8:2 to carry out a fermentation process with the brewer's yeast strain to form the nutrient composition. The nutritional composition according to claim 1 is used for villus hyperplasia, wherein 1 gram of the nutritional composition is added per kilogram of feed and mixed and fed to sea bass. A nutrient composition is used to reduce the feed conversion rate of sea bass, wherein the nutrient composition is formed by a soybean culture medium, an Antrodia cinnamomea extract through a fermentation process of a brewer's yeast strain, and the nutrient composition is further Contains a small molecule peptide, the small molecule peptide is below 3kDa, and the preparation method of the nutritional composition includes: Take a soybean protein and perform a hydrolysis process with a protease and a cellulase to form the soybean culture medium; and The soybean culture medium and the Antrodia cinnamomea extract are taken in a ratio of 8:2 to carry out a fermentation process with the brewer's yeast strain to form the nutrient composition. The nutritional composition described in claim 3 is used for reducing the feed conversion rate of sea bass, wherein 1 gram of the nutritional composition is added per kilogram of feed and mixed and fed to sea bass. A nutritional composition for reducing the body fat of sea bass, wherein the nutritional composition is formed by a soybean culture medium, an Antrodia cinnamomea extract through a fermentation process of a brewer's yeast strain, and the nutritional composition further comprises A small molecule peptide, the small molecule peptide is below 3kDa, and the preparation method of the nutritional composition includes: Take a soybean protein and perform a hydrolysis process with a protease and a cellulase to form the soybean culture medium; and The soybean culture medium and the Antrodia cinnamomea extract are taken in a ratio of 8:2 to carry out a fermentation process with the brewer's yeast strain to form the nutrient composition. The nutritional composition according to claim 5 is used for reducing the body fat of sea bass, wherein 1 g of the nutritional composition is added per kilogram of feed and mixed and fed to sea bass. A nutrient composition for increasing the muscle area of sea bass, wherein the nutrient composition is formed by a soybean culture medium, an Antrodia cinnamomea extract through a fermentation process of a brewer's yeast strain, and the nutrient composition further comprises A small molecule peptide, the small molecule peptide is below 3kDa, and the preparation method of the nutritional composition includes: Take a soybean protein and perform a hydrolysis process with a protease and a cellulase to form the soybean culture medium; and The soybean culture medium and the Antrodia cinnamomea extract are taken in a ratio of 8:2 to carry out a fermentation process with the brewer's yeast strain to form the nutrient composition. The nutritional composition described in claim 7 is used for increasing the muscle area of sea bass, wherein 1 gram of the nutritional composition is added per kilogram of feed and mixed and fed to the sea bass.

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