CN110604234A - Application of Chlorella sorokiniana in functional feed for improving health condition of fishes - Google Patents
Application of Chlorella sorokiniana in functional feed for improving health condition of fishes Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The invention relates to application of Chlorella sorokiniana (Chlorella sorokiniana) in a functional feed for improving health conditions of fishes, wherein the Chlorella sorokiniana (Chlorella sorokiniana) is a starch-deficient mutant GT-1-SLM2 with the preservation number of CGMCC No.13862 and is preserved in the China general microbiological culture Collection center (CGMCC, the address of which is China institute of microbiology, institute of academy of sciences, No. 3, North Chen West Lu 1 institute of North Chen, North Yan, Beijing). The chlorella sorokiniana is added into the fish feed as starch-deficient mutant strain algae powder, not only can replace part of fish meal, but also can remarkably improve the food intake, growth and weight gain speed, oxidation resistance and immunity of fish, can replace the traditional antibiotics, and avoids the problems of ecological environment damage and food safety caused by the large-scale application of the antibiotics in the feed. Therefore, the invention provides a brand new, green and safe immunopotentiator, phagostimulant or antioxidant.
Description
Technical Field
The invention relates to the technical field of aquaculture, in particular to application of chlorella sorokiniana in a functional feed for improving the health condition of fishes.
Background
According to the statistics of FAO latest data, the yield of the world aquaculture animals in 2016 is 8000 ten thousand tons, and the yield of the world aquaculture animals in China reaches 4951 ten thousand tons, which accounts for the world culture62 percent of the output of the fish culture[1]. Aquaculture is one of the fastest growing agricultural sectors, however, the intensive degree of aquaculture, the deterioration of water quality and the high incidence of diseases are the back of the rapid development[2]. According to statistics, the annual loss of aquatic animals in China caused by diseases is as high as 100-[2,3]. In the face of high-incidence diseases, the use of antibiotics is the most common coping strategy for aquaculture practitioners; however, although the use of antibiotics promotes the growth of fish and reduces the occurrence of diseases, it also causes various problems such as the generation of drug-resistant bacteria, environmental pollution and food safety[3-5]. In view of this, the practice of using antibiotics as conventional feed additives has been or is being banned in many countries[6]The search for new, green and safe antibiotic substitutes (immunopotentiators) becomes a hot spot for research of researchers and feed enterprises[7-12]。
The rainbow trout is a worldwide important bred cold water fish, has the characteristics of tender meat, nutrition, deliciousness, no fishy smell, no muscle thorns and the like, and is popular with consumers. In China, rainbow trout breeding is mainly distributed in Heilongjiang, Liaoning, Yunnan, Guizhou and other places[13]. As with most aquatic animals, rainbow trout farming also suffers from high incidence of diseases such as infectious hematopoietic necrosis, bacterial gill rot, saprolegniasis, ichthyophthiriasis, and the like[14-17]. Therefore, the search for green and effective immunopotentiators is crucial to the healthy development of the rainbow trout breeding industry.
Disclosure of Invention
Technical problem to be solved
In order to solve the problems in the prior art, the invention provides the application of the chlorella sorokiniana in the functional feed for improving the health condition of the fish, and the chlorella sorokiniana is applied to the culture process of the fish in the form of the feed or the feed additive, so that the normal growth and the better health condition of the fish are improved and ensured, the probability of disease occurrence is reduced, and the protective navigation is provided for aquatic agriculture such as fish culture and the like.
(II) technical scheme
In order to achieve the purpose, the invention adopts the main technical scheme that:
the application of the Chlorella sorokiniana in the functional feed for improving the health condition of fishes is characterized in that the Chlorella sorokiniana is a starch-deficient mutant GT-1-SLM2 with the preservation number of CGMCC No.13862 and the preservation date of 2017, 3 and 17 days. Is preserved in China general microbiological culture Collection center (CGMCC, China academy of sciences institute of microbiology No. 3, Xilu No.1, Beijing, Chaoyang, North Cheng, China).
According to the application provided by the invention, the improvement of health conditions comprises the improvement of food intake of fishes, the promotion of weight growth speed of fishes, the improvement of antioxidant enzyme activity in fishes and the enhancement of immunity of fishes.
According to the application provided by the invention, the chlorella sorokiniana is prepared into a functional feed for fish or an additive of the functional feed used as a feed.
According to the application provided by the invention, the chlorella sorokiniana starch-deficient mutant strain algae powder is used as an immunopotentiator, a phagostimulant or an antioxidant in functional feed.
According to the application provided by the invention, the chlorella sorokiniana is added into the feed in the form of dry algae powder and used as a feed additive, and when the addition proportion is more than or equal to 5 wt%, preferably 5 wt%, the culture effect and the cost of the feed can be considered.
According to the application provided by the invention, the fish is rainbow trout.
The invention also relates to a feed formula for rainbow trout, which contains 5 percent of chlorella sorokiniana as starch-deficient mutant strain dried algae powder; the Chlorella sorokiniana is a starch-deficient mutant GT-1-SLM2 with the preservation number of CGMCC No.13862, which is preserved in China general microbiological culture Collection center (CGMCC, with the address of CGMCC: China academy of sciences, North China academy of sciences, No. 3, West Lu 1 institute of Tokyo Wenyao, Chaozhou, Japan).
Further, the rainbow trout feed formula comprises the following components in percentage by mass: 28.4% fish meal, 16% soybean meal, 9% gluten meal, 4% blood meal, 26.4% flour, 5% chlorella sorokiniana as a premix of starch deficient mutant strain dry algae powder, 1% linseed oil, 9.2% soybean oil, and 1% vitamins and mineral salts; in the feed formula, the content of crude protein is 47.3-47.5%, and the content of crude fat is 16.6-16.8%.
The invention also relates to the application of the Chlorella sorokiniana in the functional feed for improving the health condition of livestock and poultry, wherein the Chlorella sorokiniana (Chlorella sorokiniana) is a starch-deficient mutant GT-1-SLM2 with the preservation number of CGMCC No.13862 and is preserved in the common microorganism center of China Committee for culture Collection of microorganisms (CGMCC, the address: China institute of academy of sciences, No. 3, North Chen West Lu No.1 institute of China, Kyoho, Beijing); the application refers to the preparation of the chlorella sorokiniana into functional feed for livestock and poultry or a functional additive used as the feed; the health condition improvement comprises the improvement of the food intake of the livestock and poultry, the promotion of the weight growth speed of the livestock and poultry, the improvement of the antioxidant enzyme activity in the livestock and poultry and/or the enhancement of the immunity of the livestock and poultry.
The functional feed can meet the basic requirements of the growth and life of aquatic fishes and livestock and poultry, has the functions of regulating the organism function and metabolism of animals in a targeted manner, and has the specific biological functions of improving the health level of the animals, producing functional fish or livestock and poultry products, reducing environmental pollution and the like.
The description of Chlorella sorokiniana as starch deficient mutant GT-1-SLM2 can be found in the related application published by the previous patent, China patent application publication No. CN107118968A, published as 9/1/2017.
(III) advantageous effects
The invention has the beneficial effects that:
the chlorella sorokiniana is used as starch-deficient mutant strain algae powder and added into fish feed, so that the chlorella sorokiniana can replace part of fish meal, and the growth, oxidation resistance and immunity of cultured fish can be obviously improved.
The invention also provides a feed formula for the rainbow trout, and cultivation experiments prove that after the rainbow trout is cultivated for 90 days by using the feed disclosed by the invention, the rainbow trout has very natural and beautiful appearance, healthy and fresh skin color and patterns, higher food intake of the fish, high growth speed, low feed coefficient, high in-vivo antioxidant enzyme activity and stronger autoimmunity.
The invention uses the chlorella sorokiniana as starch-deficient mutant strain algae powder as a feed additive to replace antibiotics used in aquaculture feed, thereby not only promoting the growth of fish and reducing the incidence of diseases, but also avoiding the problems of ecological environment pollution and food safety caused by the antibiotics. Therefore, the chlorella sorokiniana is a starch-deficient mutant strain algae powder and is a green and safe antibiotic substitute (immunopotentiator).
Drawings
The left side of the figure 1 is the control group feed, and the right side of the figure 1 is the experimental group feed; both are sinking swelling materials and lipids such as nitrogen. The experimental group feed contained 5 wt% of chlorella sorokiniana starch deficient mutant strain powder, and the control group did not contain the chlorella sorokiniana starch deficient mutant strain powder.
FIG. 2 is a photograph of fish before and after a culture experiment showing the comparison of fish sizes; the initial weight of the fish body is about 165g, and the weight of the fish body is about 365g at the end of the experiment.
FIG. 3A is a graph comparing the effect on feed intake of rainbow trout when fed to control and experimental groups; FIG. 3B is a graph comparing the effect of feed feeding on the growth rate of rainbow trout in control and experimental groups; fig. 3C is a comparison of the use of the feed when the control group and the experimental group were fed with the feed (feed factor comparison).
FIG. 4 shows the effect of feed on the antioxidant capacity of liver of rainbow trout in the control and experimental groups, including SOD value (corresponding to A in FIG. 4), GPX value (corresponding to B in FIG. 4) and CAT value (corresponding to C in FIG. 4). Remarking: SOD, superoxide dismutase; GPX, glutathione peroxidase; CAT, catalase.
FIG. 5 shows the effect of feed feeding on spleen immunity index of rainbow trout in control and experimental groups, including C3 value (corresponding to A in FIG. 5) and immunoglobulin M (corresponding to B in FIG. 5). Remarking: c3, complement 3; IgM, immunoglobulin M.
Detailed Description
For the purpose of better explaining the present invention and to facilitate understanding, the present invention will be described in detail by way of specific embodiments with reference to the accompanying drawings.
Example 1
(1) Culture of chlorella sorokiniana starch-deficient mutant GT-1-SLM2 (preservation number CGMCC No.13862)
The formula of the culture medium for heterotrophic culture is as follows:
in the basal culture solution and the supplementary culture solution: 20g/L of glucose; KNO3 2g/L；KH2PO41.2 g/L；MgSO4·7H2O 1.2g/L；Trisodium Citrate 0.2g/L；CaCl2·2H2O mother liquor (1000X) 1 ml; FeSO4·7H21mL of EDTA-2 Na mother liquor (1000X); 1mL of microelement mother liquor (1000X);
CaCl2preparing a mother solution: 105g of CaCl2·2H2O dissolved in 1000ml H2O；
FeSO4·7H2O and Na2Preparing an EDTA mother solution: 16g FeSO4·7H2O and 2.1g Na2EDTA in 1000ml H2O；
The microelement mother liquor comprises the following components: h3BO32.86g/L，ZnSO4·7H2O 0.222g/L， MnCl2·4H2O 1.81g/L，NaMoO40.021g/L，CuSO4·2H2O is 0.07 g/L. The pH was adjusted to 6.5.
The culture method comprises the following steps:
first-stage culture: activating chlorella species GT-1-SLM2 in Endo liquid medium for 2 days;
secondary culture: adding fresh Endo culture medium when the cells grow into the logarithmic phase of growth, diluting the algae liquid by 5 times, and culturing in a shake flask at 34-40 ℃ for 3 days;
and (3) third-stage culture: adding fresh Endo culture medium, diluting the algae solution by 10 times, culturing in shake flask at 29-34 deg.C for 3-4 days, and transferring the algae solution into fermenter.
Fermentation culture: fermenting in a fermentation tank at 28-32 deg.C, pH 6.2-6.7, and dissolved oxygen 38-42%, maintaining glucose in culture medium in the fermentation tank at 20g/L, culturing for 6-7 days, concentrating, centrifuging, harvesting, spraying powder, and drying to obtain starch-deficient mutant powder of Chlorella sorokiniana.
(2) Biochemical composition detection
Biochemical composition analysis was performed on the starch deficient mutant strain powder of Chlorella sorokiniana harvested by the culture in (1), and the results are shown in Table 1:
TABLE 1 Biochemical composition
As can be seen from Table 1, the starch deficient mutant strain of Chlorella sorokiniana contains well balanced nutrients, 32.39% protein, 32.96% total fatty acids and 11.47% carbohydrate.
(3) Feed production
The feed is prepared from fish meal, soybean meal, rice flour and blood powder as protein sources, flour as carbohydrate source, and linseed oil and soybean oil as fat sources, and is designed into two kinds of feeds with equal nitrogen and other fats, one kind of feed is an experimental group feed, and the other kind of feed is a control group feed. (see Table 2 for formulation ratios).
TABLE 2 cultivation experiment feed formulation (each numerical unit is mass percent)
In the feed of the experimental group, due to the addition of the chlorella sorokiniana starch-deficient mutant strain algae powder (the addition amount is 5 wt%), the increase of protein and grease of the feed is caused, and the feed of the control group is adjusted by adopting fish meal and soybean oil, so that the feed of the experimental group and the feed of the control group conform to the principle of equal nitrogen and other grease.
The chlorella sorokiniana starch-deficient mutant strain algae powder replaces 5.33% of fish meal compared with a control group. The Fish meal used in the control group and the experimental group is danish Sanjiu Fish meal (TripleNine Fish Protein), which contains 71 percent of crude Protein and 8 percent of water; the cereal powder is lotus flower tablet and contains 78.2% of crude protein; the soybean meal is produced by feed protein limited company of Sanhehui Fu grain and oil group; the blood powder is produced by Beijing Hongyun biological technology limited company, and the crude protein is more than or equal to 90 percent; the flour is produced by Nankou flour factories in Beijing, and each 100g of flour contains 1520kJ energy; the soybean oil is Koufu brand pure fragrant soybean oil (for eating and drinking); the oleum Lini is oleum Sesami of Hongjingyuan brand; the vitamin and mineral salt premix was from beijing intein huier biotechnology limited.
Before the feed is prepared, part of raw materials (soybean meal and fish meal) in the formula are crushed, then are uniformly mixed according to the proportion in the table 2, are added with water (25 percent) for adjustment, and are prepared into sinking expanded feed by using a TSE65 double-screw expander (produced by Beijing modern ocean engineering and technology development Co., Ltd.) as shown in figure 1.
(4) Cultivation experiment
The rainbow trout is bred by applying the feed of the experimental group and the control group. In particular to a method for breeding 2 rainbow trouts in two large-scale water tanks capable of supplying oxygen and controlling temperature respectively. During the culture period, the average water temperature of the fish tank is kept at 9.33 ℃, the dissolved oxygen is 8.83mg/L, the natural pH of normal water quality and the total ammonia nitrogen are 0.05mg/L, and a culture experiment is carried out for 90 days under the condition of natural illumination, so that the application effect of the chlorella sorokiniana starch defective mutant strain algae powder in the aspect of culturing the rainbow trout is researched.
(5) Application effects
(5.1) Effect on feeding, growth and feed utilization
During the experiment, the rainbow trout grows well. The weight of the fish fed with the feed of the experimental group increased from 165g initially to 363g (fig. 2) with an average water temperature of only 9.3 ℃. Meanwhile, the experimental group feed has stable quality, and the feed coefficient is about 0.94 (fig. 3C).
Compared with the control group feed, after 5% of chlorella sorokiniana starch-deficient mutant strain algae powder is added into the experimental group feed, the feeding rate and the weight gain rate of the rainbow trout fed by the experimental group are respectively improved by 9.26% (figure 3A) and 17.30% (figure 3B).
And as shown in figure 2, after the rainbow trout is cultured by the feed of the experimental group for 90 days, the rainbow trout has dark pattern color, very natural and beautiful pattern and fresh and healthy appearance.
The feed coefficient is also called bait coefficient, and refers to the weight of feed consumed by a culture object to increase one unit weight. The specific calculation method comprises the following steps: the feed coefficient is equal to the feed consumption/weight gain multiplied by 100 percent; the lower the feed coefficient is, the higher the feed conversion rate is, and the better the feed using effect is.
(5.2) Effect on Oxidation resistance
The liver is an important metabolic organ in fish. Compared with a control group, the feed added with 5% of chlorella solomorpha starch defective mutant strain algae powder can obviously improve the antioxidant enzyme activity of the liver of the rainbow trout: increasing superoxide dismutase by 8.33% (as in A of FIG. 4); increase glutathione peroxidase activity by 9.09% (as shown in B of figure 4); increase catalase activity 106.82% (see C in FIG. 4).
(5.3) Effect on immunization
Spleen is an important immune organ. Compared with a control group, the feed added with 5 percent of chlorella sorokiniana starch deficient mutant strain algae powder can obviously enhance the immunity of fish bodies: increase lysozyme activity by 9.34% (see A in FIG. 5); increasing complement C3 content by 36.32% (see B in fig. 5); increase the content of immunoglobulin IgM by 14.52% (see C in FIG. 5).
Example 2
Example 2 the addition amount of the chlorella sorokiniana starch deficient mutant strain algae powder in the feed of the experimental group of example 1 is reduced from 5% to 2%, and the feed of the control group is adjusted by fish meal and soybean oil, so that the feed of the experimental group and the feed of the control group conform to the principle of equal nitrogen and equal lipid.
Experimental results show that after 2% of chlorella sorokiniana starch-deficient mutant strain algae powder is added into feed as an additive, the feed has certain improvement effects such as improvement or enhancement on food intake, growth and weight gain speed, in-vivo antioxidant enzyme and fish immunity of fish, but the improvement effects are not as obvious as those of example 1.
Example 3
The formula composition of the experimental group feed and the control group feed in the example 1 is applied to carp breeding. In particular to a method for cultivating 2 carps in two large-scale water tanks capable of supplying oxygen and controlling temperature respectively. During the culture period, the average water temperature of the fish tank is kept at 9.33 ℃, the dissolved oxygen is 8.83mg/L, the natural pH of normal water quality and the total ammonia nitrogen are 0.05mg/L, and a culture experiment is carried out for 90 days under the condition of natural illumination, so that the application effect of the chlorella sorokiniana starch defective mutant strain algae powder in the aspect of carp culture is researched.
As a result of the experiment, the weight of the fish fed by the feed of the experimental group is increased from 150g initially to 400 g. Meanwhile, the experimental group feed has stable quality, and the feed coefficient is about 0.85. The feeding rate and the weight gain rate of the carps fed by the feed of the experimental group are respectively improved by 10.24 percent and 21.53 percent.
Compared with a control group, the feed feeding of the experimental group improves the activity of carp liver superoxide dismutase by 10.21 percent; the activity of glutathione peroxidase is improved by 14.32 percent; the catalase activity was increased 109.21%.
Compared with a control group, the feed of the experimental group improves the carp spleen lysozyme activity by 9.97 percent; increase 38.65% of the content of complement C3; the content of immune globulin IgM is increased by 17.29%.
The experiments show that the chlorella sorokiniana starch defective mutant strain algae powder is added into the feed as an additive, so that the food intake of the fish is obviously improved, the growth and weight increment speed of the fish is accelerated, the in-vivo antioxidant enzyme activity is enhanced, and the immunity of the fish is obviously improved. Therefore, researches show that the chlorella sorokiniana starch-deficient mutant strain algae powder can be used as an immunopotentiator for enhancing the immunity of fishes, and can be used as a substitute of antibiotics for enhancing the growth and health of aquatic animals. In addition, researches show that the chlorella sorokiniana starch-deficient mutant strain algae powder can play a role of a phagostimulant and an antioxidant in aquatic products, can promote the growth of fishes and can improve the antioxidant capacity of organisms.
Although the above examples take the cultivation of rainbow trout and carp as examples for verification and research, it can be understood that the chlorella sorokiniana starch deficient mutant strain algae powder is rich in various bioactive substances (such as essential amino acids, vitamins, mineral salts, unknown growth factors, total carotenoids, etc.), and when being added to other aquatic fishes/livestock and other feeds as an additive, the chlorella sorokiniana starch deficient mutant strain algae powder also has the effects of a phagostimulant and an antioxidant, and can exert similar technical effects of 'increasing food intake, accelerating growth and weight gain speed, enhancing in vivo antioxidant enzyme activity and improving immunity'. Therefore, the present invention is not limited to the use of the chlorella sorokiniana starch-deficient mutant strain powder as an additive to fish farming feed. In other words, all the schemes of using the chlorella sorokiniana starch-deficient mutant strain algae powder as a functional feed additive for improving health conditions, such as "improving food intake, increasing growth and weight gain speed, enhancing antioxidant enzyme activity in vivo and improving immunity" are all included in the scope of the present invention.
Reference to the literature
[1]FAO,The state of world fisheries and aquaculture:meeting the sustainable development goals.Food and Agriculture Organization of the UnitedNations,Rome,Italy,2018.
[2]X.Wang,Y.Lu,L.Wang,X.Li,S.Li,C.Jian,Y.Xu,Development of antibiotic alternatives on prophylactic and therapeutic treatment for bacterialdiseases,Feed and husbandary(4)(2015)18-22.
[3]X.Liu,J.C.Steele,X.-Z.Meng,Usage,residue,and human health risk of antibiotics in Chinese aquaculture:A review,Environmental Pollution 223(Supplement C)(2017)161-169.
[4]F.C.Cabello,Heavy use of prophylactic antibiotics in aquaculture:a growing problem for human and animal health and for the environment,Environmental microbiology 8(7)(2006)1137-1144.
[5]K.Kümmerer,Antibiotics in the aquatic environment–A review–Part I,Chemosphere 75(4)(2009)417-434.
[6]M.A.O.Dawood,S.Koshio,M.Esteban,Beneficial roles of feed additives as immunostimulants in aquaculture:a review,Reviews in Aquaculture0(1)(2017)1-25.
[7]N.Van Hai,The use of medicinal plants as immunostimulants in aquaculture:A review,Aquaculture 446(2015) 88-96.
[8]S.K.Song,B.R.Beck,D.Kim,J.Park,J.Kim,H.D.Kim,E.Prebiotics as immunostimulants in aquaculture:A review,Fish&Shellfish Immunology 40(1)(2014)40-48.
[9]D.P.Anderson,Immunostimulants,adjuvants,and vaccine carriers in fish:Applications to aquaculture,Annual Review of Fish Diseases 2(1992)281-307.
[10]A.L.Gannam,R.M.Schrock,Immunostimulants in Fish Diets,Journal of Applied Aquaculture 9(4)(1999) 53-89.
[11]M.Sakai,Current research status of fish immunostimulants,Aquaculture 172(1)(1999)63-92.
[12]M.A.O.Dawood,S.Koshio,M.Esteban,Beneficial roles of feed additives as immunostimulants in aquaculture:a review,Reviews in Aquaculture10(4)(2018)950-974.
[13]Fishery Bureau,Ministry of Agriculture,People’s Republic of China,China Fishery Statistical Yearbook 2017.China Agriculture Press,Beijing,2017.
[14]E.J.Noga,Fish disease:diagnosis and treatment.Blackwell Publishing,Inc.,New Jersey,USA,2010.
[15]J.Xueqin,P.W.Kania,K.Buchmann,Comparative effects of four feed types on white spot disease susceptibility and skin immune parameters inrainbow trout,Oncorhynchus mykiss(Walbaum),Journal of Fish Diseases 35(2)(2012)127-135.
[16]I.Dalsgaard,L.Madsen,Bacterial pathogens in rainbow trout,Oncorhynchus mykiss(Walbaum),reared at Danish freshwaterfarms,Journal of Fish Diseases 23(3)(2000)199-209.
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Claims (9)
1. The application of the chlorella sorokiniana in the functional feed for improving the health condition of fishes is characterized in that: the Chlorella sorokiniana (Chlorella sorokiniana) is a starch-deficient mutant GT-1-SLM2 with the preservation number of CGMCC No.13862, which is preserved in the China general microbiological culture Collection center (CGMCC, with the address of CGMCC: China academy of sciences, North China institute of sciences, No. 3, West Lu No.1 institute of Tokyo West Chen, the area of the rising Yang in Beijing city).
2. Use according to claim 1, characterized in that: the improving health condition comprises increasing food intake of fish, promoting weight gain of fish, increasing antioxidant enzyme activity in fish, and/or enhancing immunity of fish.
3. Use according to claim 2, characterized in that: the application refers to the preparation of the chlorella sorokiniana into functional feed for fish or an additive of the functional feed for feed.
4. Use according to claim 3, characterized in that: the chlorella sorokiniana starch-deficient mutant strain algae powder is used as an immunopotentiator, a phagostimulant or an antioxidant in functional feed.
5. Use according to claim 2, characterized in that: the chlorella sorokiniana is added into feed in the form of dry algae powder and is used as a feed additive, and the addition proportion is more than or equal to 5 wt%.
6. Use according to any one of claims 1 to 5, characterized in that: the fish is rainbow trout.
7. The feed formula of the rainbow trout is characterized in that the feed formula contains 5 percent of chlorella sorokiniana which is starch-deficient mutant strain dry algae powder; the Chlorella sorokiniana is a starch-deficient mutant GT-1-SLM2 with the preservation number of CGMCC No.13862, which is preserved in China general microbiological culture Collection center (CGMCC, with the address of CGMCC: China academy of sciences, North China academy of sciences, No. 3, West Lu 1 institute of Tokyo Wenyao, Chaozhou, Japan).
8. The rainbow trout feed formulation according to claim 7, which comprises, in mass percent: 28.4% fish meal, 16% soybean meal, 9% gluten meal, 4% blood meal, 26.4% flour, 5% chlorella sorokiniana as a premix of starch deficient mutant strain dry algae powder, 1% linseed oil, 9.2% soybean oil, and 1% vitamins and mineral salts; in the feed formula, the content of crude protein is 47.3-47.5%, and the content of crude fat is 16.6-16.8%.
9. The application of the chlorella sorokiniana in the functional feed for improving the health condition of livestock and poultry is characterized in that: the Chlorella sorokiniana (Chlorella sorokiniana) is a starch-deficient mutant GT-1-SLM2 with the preservation number of CGMCC No.13862, which is preserved in the common microorganism center of China Committee for culture Collection of microorganisms (CGMCC, the address of which is China academy of sciences microorganism research institute No. 3, West Lu No.1 Hospital, Tokyo, Chaoyang district); the application refers to the preparation of the chlorella sorokiniana into functional feed for livestock and poultry or a functional additive used as the feed; the health condition improvement comprises the improvement of the food intake of the livestock and poultry, the promotion of the weight growth speed of the livestock and poultry, the improvement of the antioxidant enzyme activity in the livestock and poultry and/or the enhancement of the immunity of the livestock and poultry.
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|---|---|---|---|---|
| CN113678942A (en) * | 2021-08-04 | 2021-11-23 | 福建师范大学 | Application of chlorella |
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| CN113678942A (en) * | 2021-08-04 | 2021-11-23 | 福建师范大学 | Application of chlorella |
| CN114196544A (en) * | 2021-08-04 | 2022-03-18 | 北部湾大学 | Chlorella and chlorella powder and preparation method thereof |
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