CN102558381B - Method for extracting water-soluble soybean polysaccharide from low-temperature bean pulp through microwave-ultrasonic synergism - Google Patents
Method for extracting water-soluble soybean polysaccharide from low-temperature bean pulp through microwave-ultrasonic synergism Download PDFInfo
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- CN102558381B CN102558381B CN 201210034410 CN201210034410A CN102558381B CN 102558381 B CN102558381 B CN 102558381B CN 201210034410 CN201210034410 CN 201210034410 CN 201210034410 A CN201210034410 A CN 201210034410A CN 102558381 B CN102558381 B CN 102558381B
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Abstract
The invention discloses a method for extracting water-soluble soybean polysaccharide from low-temperature bean pulp by a microwave-ultrasonic synergistic technology. The method comprises the following steps of: pre-treating the low-temperature bean pulp by an alkaline extraction method, removing most of protein components from the low-temperature bean pulp, adding water in an amount which is 3 to 10 times the mass of wet residue, regulating the pH value to be 4 to 8, extracting by using a microwave-ultrasonic synergistic extractor, controlling the microwave power of the extraction to be 250 to 300W, the ultrasonic power to be 150 to 200w and the extraction time to be 1 to 30min, and after the extraction is finished, centrifuging, concentrating, performing ethanol extraction, centrifuging, and drying to obtain the water-soluble soybean polysaccharide. The industrial low-temperature bean pulp is taken as a raw material, a microwave and ultrasonic superposition effect is fully utilized, and the method has the advantages of low process energy consumption, short extraction time and high extraction rate, and is an environment-friendly method for extracting the water-soluble soybean polysaccharide.
Description
Technical field
The present invention relates to a kind of extraction process of polysaccharide, particularly take low temperature soy meal as raw material, except on the basis of Deproteinization, adopt the microwave-ultrasonic coordination technique to extract the method for water soluble soybean polysaccharide.
Background technology
Low temperature soy meal is the residue after the industrial production soybean oil, contain abundant polysaccharide fraction (30% left and right) and protein ingredient (40% left and right), and the water soluble soybean polysaccharide component concentration is abundant, accounts for 2/3 of polysaccharide fraction, is the desirable feedstock of extracting water soluble soybean polysaccharide.
The traditional technology that water soluble soybean polysaccharide extracts has: the hot water extraction, and alkaline process extracts, and organic acid extracts, composite enzyme extraction, membrane separation process, subcritical water extraction method etc.The factors such as energy consumption is large, extraction yield is low become the difficult problem of puzzlement water soluble soybean polysaccharide extraction process.Although the microwave extraction technology that emerges in recent years and ultrasonic technology have reduced the large difficult problem of energy consumption to a certain extent, the extraction yield of water soluble soybean polysaccharide is not significantly improved.On the other hand, along with going deep into of research, the good functional property of water soluble soybean polysaccharide makes it receive increasing concern, and particularly aspect the stability that strengthens milky-drinks, improvement Food Quality and food fresh keeping and anti-oxidation tech, application prospect is very wide.But China does not still have the industrial production line of water soluble soybean polysaccharide, makes water soluble soybean polysaccharide production and application have been subject to serious restriction.
In order to obtain water soluble soybean polysaccharide with more efficient, energy-conservation method, this research and probe a kind of take low temperature soy meal as raw material, adopt the microwave-ultrasonic coordination technique to extract the method for water soluble soybean polysaccharide.
Summary of the invention
The objective of the invention is in order to overcome the shortcomings such as in the water soluble soybean polysaccharide traditional extraction technique, the time is long, energy consumption is large, extraction yield is low, propose a kind of fast, the method for high efficiency extraction water soluble soybean polysaccharide, and this method is easy and simple to handle, extraction efficiency is high, energy consumption is low, pollution-freely be easy to realize industrialization.
For reaching above-mentioned target, the present invention adopts following technical scheme:
A kind of collaborative method of extracting water soluble soybean polysaccharide from low temperature soy meal of microwave-ultrasonic comprises the following steps:
1) low temperature soy meal and water are compared mixing according to 1: 5～10 quality, adding alkali lye to regulate pH is 8～10 to extract protein component, extract 40～50min under 50～60 ℃, centrifugation after extraction is completed is got wet residue as the raw material (supernatant liquor can further precipitate and obtain the soybean protein isolate component) that extracts water soluble soybean polysaccharide;
2) add the distilled water of 3～10 times of wet residue quality in the above-mentioned wet residue, it is 4～8 that acid adding is adjusted to pH, makes the water soluble soybean polysaccharide extracting solution;
3) said extracted liquid is placed in the collaborative extractor of microwave-ultrasonic, it is 250～300w that microwave power is set, and ultrasonic power is 150～200w, and extraction time is 1-30min;
4) extract complete rear centrifugation, get supernatant liquor, be concentrated into 1/4～1/3 of original volume, get concentrated solution;
5) the gained concentrated solution is dry through alcohol precipitation, separation, obtains water soluble soybean polysaccharide.
A preferred implementation of such scheme is, step 2) in institute to add distilled water be 4～6 times of wet residue quality, the pH of adjusting extracting solution is 6～7.
A preferred implementation of such scheme is, step 3) in extraction time be set to 10～20min.
Principle of the present invention is to adopt alkaline extraction to remove the protein ingredient in low temperature soy meal, wet residue component after extraction albumen is adopted the microwave ultrasonic wave coordination technique, superpose simultaneously microwave high energy efficiency with and hyperacoustic mechanical vibration and cavatition, promote the stripping of water soluble soybean polysaccharide component in residue, and then be settled out target components with ethanol, obtain water soluble soybean polysaccharide after vacuum lyophilization.
The present invention organically combines the microwave and ultrasound technology, and on the one hand, the high energy effect of microwave can improve the dissolution efficiency of water soluble soybean polysaccharide; Simultaneously, the mechanical vibration effect of ultrasonic technology and cavatition promote breaking of cell, and then accelerated the extraction usefulness of water soluble soybean polysaccharide, so the superimposing technique that takes full advantage of microwave and ultrasound can significantly shorten the extraction time of water soluble soybean polysaccharide, improve greatly the extraction yield of water soluble soybean polysaccharide, the energy consumption that maximum reduction traditional extraction process is brought is large, heavy-polluted problem, is a kind of environmentally friendly water soluble soybean polysaccharide extracting method; In addition, the present invention obtains the soybean protein isolate product take low temperature soy meal as raw material when extracting water soluble soybean polysaccharide, realized the comprehensive development and utilization to the low temperature soy meal resource.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.
Embodiment 1
Accurately take 100g low temperature soy meal raw material, add entry 1kg stirring and evenly mixing, regulating pH is 8.50, controlling and extracting temperature is 50 ℃, it is 40min that extraction time is set, after extraction is completed, and centrifugation, the supernatant liquor precipitation obtains the soybean protein isolate component, keeps residue as the raw material that extracts water soluble soybean polysaccharide;
Add 1.8kg distilled water in the above-mentioned residue of 300g, stir, the pH that regulates extracting solution with hydrochloric acid is 4.0;
Extracting solution is placed in microwave-ultrasonic assists extractor, it is 250w that microwave extraction power is set, and ultrasonic extraction power is 200w, and it is 2min that extraction time is set;
React complete after, centrifugal, the reject residue is got the supernatant liquor vacuum concentration to 1/4 of original volume;
With 75% ethanol precipitation, 95% ethanol and absolute ethanol washing, centrifugal respectively, precipitation is carried out vacuum lyophilization, obtain water soluble soybean polysaccharide, recording extraction yield is 67.4%.
Embodiment 2
Accurately take 300g low temperature soy meal raw material, adding entry 2kg stirs, regulating pH is 8.50, controlling and extracting temperature is 55 ℃, it is 45min that extraction time is set, after extraction is completed, and centrifugation, the supernatant liquor precipitation obtains the soybean protein isolate component, keeps residue as the raw material that extracts water soluble soybean polysaccharide;
Add 3kg distilled water in the above-mentioned residue of 1000g, stir, the pH that regulates extracting solution with hydrochloric acid is 6.0;
Extracting solution is placed in microwave-ultrasonic assists extractor, it is 260w that microwave extraction power is set, and ultrasonic extraction power is 180w, and it is 5min that extraction time is set;
React complete after, centrifugal, the reject residue is got the supernatant liquor vacuum concentration to 1/4 of original volume;
With 75% ethanol precipitation, 95% ethanol and absolute ethanol washing, centrifugal respectively, precipitation is carried out vacuum lyophilization, obtain water soluble soybean polysaccharide, recording extraction yield is 79.8%.
Embodiment 3
Accurately take 200g low temperature soy meal raw material, adding entry 1.5kg stirs, regulating pH is 8.50, controlling and extracting temperature is 55 ℃, it is 40min that extraction time is set, after extraction is completed, and centrifugation, the supernatant liquor precipitation obtains the soybean protein isolate component, keeps residue as the raw material that extracts water soluble soybean polysaccharide;
Add 4kg distilled water in the above-mentioned residue of 400g, stir, the pH that regulates extracting solution with hydrochloric acid is 7.0;
Extracting solution is placed in microwave-ultrasonic assists extractor, it is 275w that microwave extraction power is set, and ultrasonic extraction power is 160w, and it is 15min that extraction time is set;
React complete after, centrifugal, the reject residue is got the supernatant liquor vacuum concentration to 1/3 of original volume;
With 75% ethanol precipitation, 95% ethanol and absolute ethanol washing, centrifugal respectively, precipitation is carried out vacuum lyophilization, obtain water soluble soybean polysaccharide, recording extraction yield is 83.5%.
Embodiment 4
Accurately take 500g low temperature soy meal raw material, add 5kg water to stir, regulating pH is 8.50, controlling and extracting temperature is 55 ℃, it is 50min that extraction time is set, after extraction is completed, and centrifugation, the supernatant liquor precipitation obtains the soybean protein isolate component, keeps residue as the raw material that extracts water soluble soybean polysaccharide;
Add 8kg distilled water in the above-mentioned residue of 1000g, stir, the pH that regulates extracting solution with hydrochloric acid is 7.0;
Extracting solution is placed in microwave-ultrasonic assists extractor, it is 285w that microwave extraction power is set, and ultrasonic extraction power is 160w, and it is 25min that extraction time is set;
React complete after, centrifugal, the reject residue is got the supernatant liquor vacuum concentration to 1/3 of original volume;
With 75% ethanol precipitation, 95% ethanol and absolute ethanol washing, centrifugal respectively, precipitation is carried out vacuum lyophilization, obtain water soluble soybean polysaccharide, recording extraction yield is 88.6%.
Embodiment 5
Accurately take 50g low temperature soy meal raw material, add 250g distilled water, regulating pH is 8.50, controlling and extracting temperature is 60 ℃, it is 40min that extraction time is set, after extraction is completed, and centrifugation, the supernatant liquor precipitation obtains the soybean protein isolate component, keeps residue as the raw material that extracts water soluble soybean polysaccharide;
Add 1kg distilled water in the above-mentioned residue of 100g, stir, the pH that regulates extracting solution with hydrochloric acid is 8.0;
Extracting solution is placed in microwave-ultrasonic assists extractor, it is 300w that microwave extraction power is set, and ultrasonic extraction power is 150w, and it is 30min that extraction time is set;
React complete after, centrifugal, the reject residue is got the supernatant liquor vacuum concentration to 1/3 of original volume;
With 75% ethanol precipitation, 95% ethanol and absolute ethanol washing, centrifugal respectively, precipitation is carried out vacuum lyophilization, obtain water soluble soybean polysaccharide, recording extraction yield is 89.3%.
Above-described embodiment is interpreted as only being used for explanation the present invention and is not used in restriction protection scope of the present invention.After the content of having read the present invention's record, those skilled in the art can make various changes or modifications the present invention, and these equivalences change and modification falls into claim limited range of the present invention equally.
Claims (1)
1. the collaborative method of extracting water soluble soybean polysaccharide from low temperature soy meal of microwave-ultrasonic, is characterized in that, comprises the following steps:
1) with low temperature soy meal and water according to the quality of 1:5～10 than mixing, adding alkali lye to regulate pH is 8～10, extracts 40～50min under 50～60 ℃, extract complete after centrifugation, get wet residue as the raw material that extracts water soluble soybean polysaccharide;
2) add the distilled water of 4～6 times of wet residue quality in the above-mentioned wet residue, it is 6～7 that acid adding is adjusted to pH, makes the water soluble soybean polysaccharide extracting solution;
3) said extracted liquid is placed in the collaborative extractor of microwave-ultrasonic, it is 250～300w that microwave power is set, and ultrasonic power is 150～200w, and extraction time is 10～20min;
4) extract complete rear centrifugation, get supernatant liquor, be concentrated into 1/4～1/3 of original volume, get concentrated solution;
5) the gained concentrated solution is dry through alcohol precipitation, separation, obtains water soluble soybean polysaccharide.
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| CN 201210034410 CN102558381B (en) | 2012-02-16 | 2012-02-16 | Method for extracting water-soluble soybean polysaccharide from low-temperature bean pulp through microwave-ultrasonic synergism |
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| CN104725519A (en) * | 2015-03-09 | 2015-06-24 | 梁永锋 | Method for extracting and purifying banana peel polysaccharide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101177460A (en) * | 2007-12-05 | 2008-05-14 | 浙江工商大学 | Method for extracting water-soluble soybean polysaccharide from soya bean waste and soybean dregs |
| CN101434660A (en) * | 2008-08-01 | 2009-05-20 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for extracting Ganoderma lucidum polysaccharide by ultrasonic wave-microwave combination |
| CN102329382A (en) * | 2011-09-05 | 2012-01-25 | 江苏省农业科学院 | Method for extracting rapeseed proteins through ultrasonic-microwave synergy |
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| JP4682377B2 (en) * | 2009-10-06 | 2011-05-11 | 独立行政法人農業・食品産業技術総合研究機構 | Extraction of sialic acid-containing compounds from plants |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101177460A (en) * | 2007-12-05 | 2008-05-14 | 浙江工商大学 | Method for extracting water-soluble soybean polysaccharide from soya bean waste and soybean dregs |
| CN101434660A (en) * | 2008-08-01 | 2009-05-20 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for extracting Ganoderma lucidum polysaccharide by ultrasonic wave-microwave combination |
| CN102329382A (en) * | 2011-09-05 | 2012-01-25 | 江苏省农业科学院 | Method for extracting rapeseed proteins through ultrasonic-microwave synergy |
Non-Patent Citations (2)
| Title |
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| 《Optimization of ultrasonic/microwave assisted extraction (UMAE) of polysaccharides from Inonotus obliquus and evaluation of its anti-tumor activities》;Yiyong Chen et al;《International Journal of Biological Macromolecules》;20100210;第46卷;全文 * |
| Yiyong Chen et al.《Optimization of ultrasonic/microwave assisted extraction (UMAE) of polysaccharides from Inonotus obliquus and evaluation of its anti-tumor activities》.《International Journal of Biological Macromolecules》.2010,第46卷429-435. |
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