CN102558378B - Preparation method of water-soluble soybean polysaccharide - Google Patents
Preparation method of water-soluble soybean polysaccharide Download PDFInfo
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Abstract
The invention discloses a preparation method of water-soluble soybean polysaccharide, which comprises the following steps of: removing the majority of protein components in low-temperature bean pulp, adding water with the ratio of wet residues and water 1: (3-10), adjusting the pH to 2.0-10.0, extracting with a microwave-ultrasonic assisted extraction device, controlling the microwave power to be 275w, the ultrasonic power to be 160w and the time to be 1-30min, carrying out centrifugation after extraction, concentrating supernatant to one fourth of the original volume, washing sequentially with ethanol of 75 percent, ethanol of 95 percent and absolute ethanol, precipitating, standing at the room temperature, carrying out centrifugation, and freeze-drying under vacuum conditions, thereby obtaining the water-soluble soybean polysaccharide with the molecular weight of 1200-2400kDa. In the preparation method of the water-soluble soybean polysaccharide, industrial low-temperature beam pulp is used as the raw material, the energy consumption for extraction process is low, the extraction time can be reduced significantly, and the extraction rate can be increased. Therefore, the method is an environmentally friendly method for extraction of the water-soluble soybean polysaccharide.
Description
Technical field
The present invention relates to a kind of extraction process of polysaccharide, particularly take low temperature soy meal as raw material, except on the basis of Deproteinization, adopt the microwave-ultrasonic coordination technique to extract the method for water soluble soybean polysaccharide.
Background technology
Low temperature soy meal is the residue after the industrial production soybean oil, contain abundant polysaccharide fraction (30% left and right) and protein ingredient (40% left and right), and the water soluble soybean polysaccharide component concentration is abundant, accounts for 2/3 of polysaccharide fraction, it is the desirable feedstock of extracting water soluble soybean polysaccharide.
The traditional technology that water soluble soybean polysaccharide extracts has: the hot water extraction, and alkaline process extracts, and organic acid extracts, composite enzyme extraction, membrane separation process, subcritical water extraction method etc., the factor such as energy consumption is large, extraction yield is low becomes the difficult problem of puzzlement water soluble soybean polysaccharide extraction process.Although the microwave extraction technology emerged in recent years and ultrasonic technology have reduced the large difficult problem of energy consumption to a certain extent, the extraction yield of water soluble soybean polysaccharide is not significantly improved.On the other hand, along with going deep into of research, the good functional property of water soluble soybean polysaccharide makes it receive increasing concern, and particularly, aspect the stability that strengthens milky-drinks, improvement Food Quality and food fresh keeping and anti-oxidation tech, application prospect is very wide.But China does not still have the industrial production line of water soluble soybean polysaccharide, make water soluble soybean polysaccharide production and application have been subject to serious restriction.
For the method with more efficient, energy-conservation obtains water soluble soybean polysaccharide, this research and probe a kind ofly take low temperature soy meal as raw material, adopt the microwave-ultrasonic coordination technique to extract the method for water soluble soybean polysaccharide.This method organically combines the microwave and ultrasound technology, and on the one hand, the high energy effect of microwave can improve the dissolution efficiency of water soluble soybean polysaccharide; Simultaneously, the mechanical vibration effect of ultrasonic technology and cavatition promote breaking of cell, and then accelerated the extraction usefulness of water soluble soybean polysaccharide, so the superimposing technique that takes full advantage of microwave and ultrasound can significantly shorten the extraction time of water soluble soybean polysaccharide, improve greatly the extraction yield of water soluble soybean polysaccharide, farthest reducing large, the heavy-polluted problem of energy consumption that traditional extraction process is brought, is a kind of environmentally friendly water soluble soybean polysaccharide extracting method.
Summary of the invention
The objective of the invention is in order to overcome the shortcomings such as in the water soluble soybean polysaccharide traditional extraction technique, the time is long, energy consumption is large, extraction yield is low, propose a kind of preparation method of water soluble soybean polysaccharide fast and efficiently, and this method is easy and simple to handle, extraction efficiency is high, energy consumption is low, pollution-freely be easy to realize industrialization.
In addition, this law be take low temperature soy meal as raw material, obtains the soybean protein isolate product when preparing water soluble soybean polysaccharide, has realized the comprehensive development and utilization to the low temperature soy meal resource.
Know-why of the present invention
A kind of preparation method of water soluble soybean polysaccharide, utilize the microwave-ultrasonic coordination technique to extract water soluble soybean polysaccharide from low temperature soy meal, being about to low temperature soy meal adopts alkali extraction and acid precipitation to remove protein ingredient, wet residue component after extraction albumen is adopted to the microwave ultrasonic wave coordination technique, superpose simultaneously microwave high energy efficiency with and hyperacoustic mechanical vibration and cavatition, promote the stripping of water soluble soybean polysaccharide component in residue, and then be settled out target components with ethanol, obtain water soluble soybean polysaccharide after vacuum lyophilization.
Technical scheme of the present invention
A kind of preparation method of water soluble soybean polysaccharide, specifically comprise the steps:
(1) according to mass ratio, be, low temperature soy meal: the ratio of water is 1:10, low temperature soy meal is added to the water, then regulating pH with 1M sodium hydroxide is 8.50, controlling extraction temperature is 55 ℃, and the time is 42.8min, after having extracted, the control centrifugal rotational speed is 4000r/min, centrifugation time is 20min, carries out centrifugation, obtains supernatant liquor and wet residue;
After crossing 40 mesh sieves, uses above-mentioned low temperature soy meal raw material;
Under the condition that the supernatant liquor of above-mentioned gained is 4.5 at pH, precipitation obtains the soybean protein isolate component;
(2), by the wet residue of step (1) gained according to mass ratio, i.e. wet residue: the ratio of water is 1:3-10, is preferably 1:5, after adding water and mix in wet residue, regulating pH with 1M hydrochloric acid or sodium hydroxide is 2.0-10.0, is preferably pH6.80, obtains reaction solution;
Above-mentioned water used is distilled water;
(3), the reaction solution of step (2) gained is placed in to the collaborative extractor of microwave-ultrasonic, it is 275w that microwave power is set, and ultrasonic power is 160w, and extraction time is 1-30min, preferably 15-20min;
(4), after step (3) extracts, controls centrifugal rotational speed is 4000r/min, centrifugal 15min, the reject residue, retain supernatant liquor, and by supernatant liquor vacuum tightness be carry out vacuum concentration to 1/4 of original volume under the 3.0kpa part must concentrated solution;
(5), by after the ethanol that is 75% by concentration successively in the concentrated solution of step (4) gained, 95% ethanol and absolute ethanol washing, precipitation at room temperature;
(6), being deposited under the 4000r/min condition of step (5) gained controlled after centrifugal 15min under vacuum tightness 13.3pa again and carried out vacuum lyophilization, finally obtain molecular weight water soluble soybean polysaccharide between 1200 kDa-2400kDa.
Technique effect of the present invention
The preparation method of a kind of water soluble soybean polysaccharide of the present invention, owing to having adopted the collaborative technology of extracting of microwave and ultrasound, the high energy effect of microwave and hyperacoustic mechanical vibration and the cavatition of simultaneously superposeing carried out the extraction of water soluble soybean polysaccharide, make water soluble soybean polysaccharide more easily leach, thereby make the extraction yield of water soluble soybean polysaccharide significantly improve, extraction rate reached 63～88%.
And, the resulting water soluble soybean polysaccharide of the preparation method of a kind of water soluble soybean polysaccharide of the present invention is compared with the water soluble soybean polysaccharide of microwave method and ultrasonic extraction method, because the enthalpy change between 63 ℃-85 ℃ maintains in the 64-79J/g scope, be microwave-ultrasonic collaborative to extract the water soluble soybean polysaccharide obtained similar to the thermal properties of the water soluble soybean polysaccharide that adopts respectively microwave technology to obtain with ultrasonic technology, so the preparation method of a kind of water soluble soybean polysaccharide of the present invention can't change the thermostability of water soluble soybean polysaccharide.
In addition, the molecular weight of the resulting water soluble soybean polysaccharide of preparation method of a kind of water soluble soybean polysaccharide of the present invention is between 1200-2400kDa, preferably between 1258-1674kDa, be less than the molecular weight (2000-2400kDa) of the water soluble soybean polysaccharide that independent employing microwave or ultrasonic technology obtain, because molecular weight is little, make the structural flexibility of the water soluble soybean polysaccharide molecule of preparation method's gained of the present invention strengthen, thereby show better functional property.
Embodiment
Below by embodiment, the present invention is further set forth, but do not limit the present invention.
The extraction yield of water soluble soybean polysaccharide of the present invention:
Extraction yield=
The CW-2000 type microwave that the present invention's microwave-ultrasonic used assists extractor to provide for Shanghai Xintuo Analytical Instruments Technology Co.ltd-supersonic synergic reaction instrument.
The present invention's other equipment used have:
Electronic balance, Beijing Sai Duolisi instrument system company limited;
Tetrad magnetic force heating stirrer, Shanghai large photoelectron Science and Technology Ltd.;
The numerical control super constant temperature trough, Ningbo Tian Heng instrument plant;
The glass jacket enzyme reactor, glasswork's customization;
PHS-25 type digital display pH meter, Shanghai Precision Scientific Apparatus Co., Ltd;
Avanti J-20XP high speed freezing centrifuge, U.S. Beckman Coulter Inc.;
WFJ7200 type visible spectrophotometer, Long Nike (Shanghai) Instrument Ltd.;
AD2.0 VIRTIS Freeze Drying Equipment, Shanghai Si Gaole bio tech ltd;
Differential scanning calorimeter EXSTAR DSC.6200, Seiko Instruments, Inc. Japan;
Nexus870 type Fourier turn infrared instrument, U.S. Necolet company;
The desk-top scanning electron microscope of Phenom (flying to receive), U.S. FEI Co.;
The L-2000 high performance liquid chromatograph, Hitachi, Ltd.
Raw material and reagent used in embodiments of the invention:
Cross the low temperature soy meal after 40 mesh sieves, Fenghua, Zhejiang flying dragon chemical industry company limited provides;
Take the water soluble soybean polysaccharide that rhamnosyl and galacturonic acid be skeleton, SSPS-20, Shanghai hundred special vegetable-protein difficult to understand Science and Technology Ltd.
Hydrochloric acid, sodium hydroxide, sulfuric acid, glucose, phenol etc. are domestic analytical pure.
In low temperature soy meal, the mensuration of total sugar content adopts the phenol sulfuric acid process
[1].
[1] Zhao Yahua, on the sunny side high. biochemical test technical tutorial [M]. Guangzhou: press of South China Science & Engineering University, 2000:40-42.
embodiment 1
A kind of preparation method of water soluble soybean polysaccharide, specifically comprise the steps:
(1), add water 1L stirring and evenly mixing in the 100g low temperature soy meal, regulating pH with 1M sodium hydroxide is 8.50, controlling extraction temperature is 55 ℃, time is 42.8min, after extraction completes, the control rotating speed is 4000r/min, centrifugal 20min, the supernatant liquor precipitation obtains the soybean protein isolate component, retains wet residue as the raw material that extracts water soluble soybean polysaccharide;
(2), in above-mentioned wet residue, add 0.3L water, stir, the pH that regulates extracting solution with 1M hydrochloric acid is 2.0, obtains reaction solution;
(3), the reaction solution of step (2) gained be placed in to microwave-ultrasonic assist extractor, it is 275w that microwave extraction power is set, ultrasonic extraction power is 160w, it is 1min that extraction time is set;
(4), after step (3) extracts, controls centrifugal rotational speed is 4000r/min, it is centrifugal that the time is that 15min carries out, the reject residue, get supernatant liquor control vacuum tightness carry out vacuum concentration to 1/4 of original volume under 0.3kpa must concentrated solution;
(5), the ethanol that is 75% by concentration successively in the concentrated solution of step (4) gained, ethanol and the dehydrated alcohol that concentration is 95% are washed concentrated solution, and precipitated under room temperature;
(5), being deposited under the 4000r/min condition of step (5) gained controlled to the capable vacuum lyophilization of vacuum tightness 13.3pa again after centrifugal 15min, finally obtain the water soluble soybean polysaccharide of 19.02g, its extraction yield is 63.4%.
It is 1258kDa that the water soluble soybean polysaccharide of above-mentioned gained is surveyed its molecular weight by high pressure lipuid chromatography (HPLC), and dsc is measured the water soluble soybean polysaccharide of gained, and the enthalpy change obtained between 63 ℃-85 ℃ maintains in the 66-71J/g scope.
The light collection of illustrative plates that records the water soluble soybean polysaccharide of above-mentioned gained by Fourier transform infrared spectrometry conforms to standard substance, shows that products obtained therefrom of the present invention is really water soluble soybean polysaccharide.
Further pass through the particle structure of the water soluble soybean polysaccharide of the above-mentioned gained of scanning electron microscopic observation, can further determine, products obtained therefrom of the present invention is water soluble soybean polysaccharide.
embodiment 2
A kind of preparation method of water soluble soybean polysaccharide, specifically comprise the steps:
(1), after adding water 3L to stir in 300g low temperature soy meal raw material, regulating pH with 1M sodium hydroxide is 8.50, controlling extraction temperature is 55 ℃, time is 42.8min, after extraction completes, the control rotating speed is 4000r/min, and the time is that 20min carries out centrifugation, the supernatant liquor precipitation obtains the soybean protein isolate component, retains wet residue as the raw material that extracts water soluble soybean polysaccharide;
(2), in the wet residue of step (1) gained, add water 1.5L, after stirring, with 1M salt acid for adjusting pH, be 6.8, obtain reaction solution;
(3), the reaction solution of step (2) gained be placed in to microwave-ultrasonic assist extractor, it is 275w that microwave extraction power is set, ultrasonic extraction power is 160w, it is 15min that extraction time is set;
(4), after step (3) extracts, controls centrifugal rotational speed is 400r/min, it is centrifugal that the time is that 15min carries out, the reject residue, get supernatant liquor control vacuum tightness be carry out vacuum concentration to 1/4 of original volume under 3.0kpa must concentrated solution;
(5), the ethanol that is 75% by concentration successively in the concentrated solution of step (4) gained, ethanol and the dehydrated alcohol that concentration is 95% are washed concentrated solution, and precipitated under room temperature;
(6), being deposited under the 4000r/min condition of step (5) gained controlled to vacuum tightness again after centrifugal 15min be that 13.3pa carries out vacuum lyophilization, finally obtains the water soluble soybean polysaccharide of 71.82g, its extraction yield is 79.8%.
It is 1674kDa that the water soluble soybean polysaccharide of above-mentioned gained is surveyed its molecular weight by high pressure lipuid chromatography (HPLC), and dsc is measured the water soluble soybean polysaccharide of gained, and the enthalpy change obtained between 63 ℃-85 ℃ maintains in the 70-79J/g scope.
embodiment 3
A kind of preparation method of water soluble soybean polysaccharide, specifically comprise the steps:
(1), after adding water 2L to stir in 200g low temperature soy meal raw material, regulating pH with 1M sodium hydroxide is 8.50, controlling extraction temperature is 55 ℃, time is 42.8min, after extraction completes, the control rotating speed is 4000r/min, and time 20min carries out centrifugation, the supernatant liquor precipitation obtains the soybean protein isolate component, retains wet residue as the raw material that extracts water soluble soybean polysaccharide;
(2), in the wet residue of step (1) gained, add 1.2L water, stirring, is 7.5 with 1M salt acid for adjusting pH, obtains reaction solution;
(3), the reaction solution of step (2) gained be placed in to microwave-ultrasonic assist extractor, it is 275w that microwave extraction power is set, ultrasonic extraction power is 160w, it is 20min that extraction time is set;
(4), after step (3) extracts, control the centrifugal 4000r/min of transferring to, it is centrifugal that the time is that 15min carries out, the reject residue, get supernatant liquor control vacuum tightness be under 3.0kpa, carry out vacuum concentration to original volume 1/4 concentrated solution;
(5), the ethanol that is 75% by concentration successively in the concentrated solution of step (4) gained, ethanol and the dehydrated alcohol that concentration is 95% are washed concentrated solution, and precipitated under room temperature;
(6), being deposited under the 4000r/min condition of step (5) gained controlled to vacuum tightness again after centrifugal 15min be that 13.3pa carries out vacuum lyophilization, finally obtains the water soluble soybean polysaccharide of 50.11g, its extraction yield is 83.5%.
It is 2094kDa that the water soluble soybean polysaccharide of above-mentioned gained is surveyed its molecular weight by high pressure lipuid chromatography (HPLC), and dsc is measured the water soluble soybean polysaccharide of gained, and the enthalpy change obtained between 63 ℃-85 ℃ maintains in the 69-78J/g scope.
embodiment 4
A kind of preparation method of water soluble soybean polysaccharide, specifically comprise the steps:
(1), in 500g low temperature soy meal raw material, add 5L water to stir after, regulating pH with 1M sodium hydroxide is 8.50, controlling extraction temperature is 55 ℃, the time is 42.8min, after having extracted, the control rotating speed is 4000r/min.Time is that 20min carries out centrifugation, and the supernatant liquor precipitation obtains the soybean protein isolate component, retains wet residue as the raw material that extracts water soluble soybean polysaccharide;
(2), in the wet residue of step (1) gained, add 3.5L water, stir, the pH that regulates extracting solution with 1M hydrochloric acid is 8.0, obtains reaction solution;
(3), the reaction solution of step (2) gained be placed in to microwave-ultrasonic assist extractor, it is 275w that microwave extraction power is set, ultrasonic extraction power is 160w, it is 25min that extraction time is set;
(4), after step (3) extracts, the control centrifugal rotational speed is 4000r/min.It is centrifugal that time is that 20min carries out, the reject residue, get supernatant liquor control vacuum tightness be under 3.0kpa, carry out vacuum concentration to original volume 1/4 concentrated solution;
(5), the ethanol that is 75% by concentration successively in the concentrated solution of step (4) gained, ethanol and the dehydrated alcohol that concentration is 95% are washed concentrated solution, and precipitated under room temperature;
(6), being deposited under the 4000r/min condition of step (5) gained controlled to vacuum tightness 13.3pa again after centrifugal 15min carry out vacuum lyophilization, finally obtain the water soluble soybean polysaccharide of 132.90g, its extraction yield is 88.6%.
It is 2287kDa that the water soluble soybean polysaccharide of above-mentioned gained is surveyed its molecular weight by high pressure lipuid chromatography (HPLC), and dsc is measured the water soluble soybean polysaccharide of gained, and the enthalpy change obtained between 63 ℃-85 ℃ maintains in the 67-79J/g scope.
embodiment 5
A kind of preparation method of water soluble soybean polysaccharide, specifically comprise the steps:
(1), add 500ml distilled water in 50g low temperature soy meal raw material after, regulating pH with 1M sodium hydroxide is 8.50, controlling extraction temperature is 55 ℃, time is 42.8min, after extraction completes, the control rotating speed is 4000r/min, and the time is that 20min carries out centrifugation, the supernatant liquor precipitation obtains the soybean protein isolate component, retains wet residue as the raw material that extracts water soluble soybean polysaccharide;
(2), in the wet residue of step (1) gained, add 0.5L water, stir, the pH that regulates extracting solution with 1M sodium hydroxide is 10.0, obtains reaction solution;
(3), the reaction solution of step (2) gained be placed in to microwave-ultrasonic assist extractor, it is 275w that microwave extraction power is set, ultrasonic extraction power is 160w, it is 30min that extraction time is set;
(4), after step (3) extracts, controls centrifugal rotational speed is 4000r/min, the time is that 15min is capable centrifugal, the reject residue, get supernatant liquor control vacuum tightness be carry out vacuum concentration to 1/4 of original volume under 3.0kap must concentrated solution;
(5), the ethanol that is 75% by concentration successively in the concentrated solution of step (4) gained, ethanol and the dehydrated alcohol that concentration is 95% are washed concentrated solution, and precipitated under room temperature;
(6), being deposited under the 4000r/min condition of step (5) gained controlled to vacuum tightness again after centrifugal 15min be that 13.3pa carries out vacuum lyophilization, finally obtains the water soluble soybean polysaccharide of 12.05g, its extraction yield is 80.3%.
It is 2458kDa that the water soluble soybean polysaccharide of above-mentioned gained is surveyed its molecular weight by high pressure lipuid chromatography (HPLC), and dsc is measured the water soluble soybean polysaccharide of gained, and the enthalpy change obtained between 63 ℃-85 ℃ maintains in the 64-76J/g scope.
From the result of the water soluble soybean polysaccharide of the above embodiments 1-5 gained, show: the collaborative processing method of employing microwave-ultrasonic of the present invention is extracted water soluble soybean polysaccharide, the cytoclasis degree is large, and extraction yield adopts more separately microwave method or supersonic method to improve significantly; Thermal properties is similar to the water soluble soybean polysaccharide that adopts microwave method or supersonic method to obtain separately, and thermostability is high; Molecular weight is 1200-2400kDa, and preferably, between 1258-1674kDa, the water soluble soybean polysaccharide molecular weight (2000-2400kDa) that adopts more separately microwave method or supersonic method to obtain is little, and the functional property of applying in food is better.
Above said content is the basic explanation under conceiving for the present invention only, and, according to any equivalent transformation that technical scheme of the present invention is done, all should belong to protection scope of the present invention.
Claims (5)
1. the preparation method of a water soluble soybean polysaccharide, is characterized in that comprising the steps:
(1), according to the mass ratio of low temperature soy meal and water, be low temperature soy meal: the ratio that water is 1:10 adds water in the low temperature soy meal raw material, then regulating pH with 1M sodium hydroxide is 8.50, controlling extraction temperature is 55 ℃, time is 42.8min, after extraction completes, centrifugation obtains wet residue and supernatant liquor;
(2), by the wet residue of step (1) gained according to the i.e. wet residue of material mass ratio: the ratio of water is 1:3-10, after adding water to mix in wet residue, with 1M hydrochloric acid or sodium hydroxide adjusting pH, is that 2.0-10.0 obtains reaction solution;
(3), the reaction solution of step (2) gained is placed in to the collaborative extractor of microwave-ultrasonic, it is 275w that microwave power is set, and ultrasonic power is 160w, and the time is that 1-30min is extracted;
(4), after step (3) is extracted, the control centrifugal rotational speed is 4000r/min, and it is centrifugal that the time is that 15min carries out, the reject residue, retain supernatant liquor, and carry out vacuum concentration to 1/4 of original volume under the condition that is 3.0KPa in vacuum tightness by supernatant liquor and obtain concentrated solution;
(5), after the ethanol that is 75% by concentration successively by the concentrated solution of step (4) gained, 95% ethanol and absolute ethanol washing, precipitation at room temperature;
(6), being deposited under the 4000r/min condition of step (5) gained controlled after centrifugal 15min under vacuum tightness 13.3Pa again and carried out vacuum lyophilization, finally obtain water soluble soybean polysaccharide.
2. the preparation method of a kind of water soluble soybean polysaccharide as claimed in claim 1, it is characterized in that the mass ratio of the wet residue described in step (2) and water is preferably according to wet residue: the ratio that water is 1:5 is mixed.
3. the preparation method of a kind of water soluble soybean polysaccharide as claimed in claim 2, is characterized in that the pH described in step (2) is preferably 6.80.
4. the preparation method of a kind of water soluble soybean polysaccharide as claimed in claim 3, is characterized in that the extraction time described in step (3) is preferably 15min.
5. as the preparation method of the arbitrary described a kind of water soluble soybean polysaccharide of claim 1-4, it is characterized in that preferably mistake 40 mesh sieves of step (1) low temperature soy meal used.
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| CN103044570B (en) * | 2013-01-25 | 2015-08-26 | 福建海兴保健食品有限公司 | A kind of extraction process of high efficiency extraction sea grass polysaccharide |
| CN103335971B (en) * | 2013-07-09 | 2015-11-18 | 顾祥茂 | The method of ultrasonic extraction red jujube polysaccharide is carried out after a kind of alkali lye lixiviate |
| CN104017099B (en) * | 2014-06-12 | 2016-04-06 | 苏州经贸职业技术学院 | Microwave extraction, separation and purification method of pea polysaccharide |
| CN111303308A (en) * | 2019-12-16 | 2020-06-19 | 福建省泉州市味博食品有限公司 | Production process of soluble soybean polysaccharide for rice and flour products |
| CN111333744B (en) * | 2020-03-30 | 2021-03-26 | 江南大学 | Method for removing ester of water-soluble soybean polysaccharide and product thereof |
| CN111742993B (en) * | 2020-06-10 | 2023-10-20 | 平顶山金晶生物科技股份有限公司 | Sugar-reducing lactobacillus beverage modifier and preparation method of lactobacillus beverage containing same |
| CN112048025A (en) * | 2020-08-31 | 2020-12-08 | 华南理工大学 | Zinc supplement water-soluble soybean polysaccharide zinc chelate as well as preparation method and application thereof |
| CN113354749A (en) * | 2021-05-14 | 2021-09-07 | 华南理工大学 | Method for extracting water-soluble soybean polysaccharide from bean dregs by using high-voltage pulse electric field in cooperation with ultrasonic |
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