CN103120249A - Method for extracting peanut protein from high-temperature peanut meals - Google Patents
Method for extracting peanut protein from high-temperature peanut meals Download PDFInfo
- Publication number
- CN103120249A CN103120249A CN2012105451376A CN201210545137A CN103120249A CN 103120249 A CN103120249 A CN 103120249A CN 2012105451376 A CN2012105451376 A CN 2012105451376A CN 201210545137 A CN201210545137 A CN 201210545137A CN 103120249 A CN103120249 A CN 103120249A
- Authority
- CN
- China
- Prior art keywords
- acid
- peanut
- high temperature
- protein
- alkali
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a method for extracting peanut protein from high-temperature peanut meals. The method comprises the following steps of: immersing the high-temperature peanut meals, carrying out steam explosion, carrying out alkali dissolution and digestion, carrying out acid precipitation and drying to obtain the peanut protein. The method disclosed by the invention has the advantages that a steam explosion technology is combined with the alkali dissolution and acid precipitation so that the extraction rate of extracting the peanut protein from the high-temperature peanut meals is improved, and the emulsifying property and the foaming property of an obtained peanut protein product are improved.
Description
Technical field
The present invention relates to a kind of steam blasting technology of utilizing and extract the method for peanut protein in conjunction with the heavy method of the molten acid of alkali from high temperature peanut meal, belong to the vegetable protein manufacture field.
Background technology
Peanut meal is the main byproduct that peanut oil is produced.The oil expression method that China most of peanut oil processing enterprise adopts is mechanical expression method and organic solvent lixiviation process, produces a large amount of high-temperature denatured defatted peanut dregs of rice.At present, high temperature peanut meal is because its protein denaturation is serious, and functional character is very poor, mainly as feed and fertilizer, causes serious waste of resources.
In peanut meal protein content is up to 40%～50%, and amino acid whose abundant species wherein contains eight kinds of essential amino acids of needed by human body, has very high nutritive value, and therefore, peanut meal is a kind of more satisfactory vegetable protein source.At present, be mainly the protein that adopts in the heavy technique extraction of traditional molten acid of alkali high temperature peanut meal both at home and abroad, extraction rate of protein is lower.In addition, the functional character and the organoleptic quality that directly adopt the heavy technique of the molten acid of alkali to extract the peanut protein that obtains from high temperature peanut meal are not good, use and have been subject to larger restriction.Therefore, if can recover by certain technology the functional character of protein in high temperature peanut meal, and improve its recovery rate, will have broad application prospects.
Steam blasting (be called for short " explosion ") technology is based on the HTHP boiling of short time, makes high steam enter the material internal voids, then material is suspended in atmosphere with fried loose form in the time range of Millisecond.This technology is mainly for the treatment of cellulose family raw materials such as stalk, forests at present, make hemicellulose be degraded into soluble sugar, lignin softens and Partial digestion simultaneously, and the fiber bonding strength reduces, thereby realize component separation and structural change, to improving enzymatic hydrolyzation and digestibility, using value is preferably arranged.
The present invention adopts the steam blasting technology in conjunction with the heavy peanut protein that extracts in high temperature peanut meal of the molten acid of alkali.Destroy peanut meal cell and institutional framework by the steam blasting technology, interrupt the chemical bond between peanut protein and other macromolecular substances, more be conducive to the extraction of albumen.Simultaneously, in the steam blasting process, Maillard reaction has occured in protein and sugar, and the key entry of glycosyl has increased the ratio of hydrophilic radical in the protein molecule, has improved the hydrophily of protein.In addition, steam blasting also makes protein structure launch, and more groups come out, and have improved flexibility and the surface-active of molecule, thereby has improved the functional characters such as dissolubility, emulsibility, foaming characteristic of protein.
The present invention proposes a kind ofly not only can improve the recovery rate that extracts peanut protein from high temperature peanut meal, but also can improve the method for product function character.
Summary of the invention
Technical problem the object of the invention is to adopt the steam blasting technology in conjunction with the heavy peanut protein that extracts in high temperature peanut meal of the molten acid of alkali, overcome under existence conditions that peanut protein recovery rate in high temperature peanut meal is low, the problem such as the functional character such as the emulsifiability of product and frothing capacity is not good, have wide market-oriented prospect.
Technical scheme adopts steam blasting in conjunction with the heavy method of extracting peanut protein in high temperature peanut meal of the molten acid of alkali, mainly take high temperature peanut meal as raw material, through immersions, steam blasting processings, the molten lixiviate of alkali, sourly sink, drying obtains peanut protein; Step is as follows:
1, the dipping pretreatment of high temperature peanut meal
Take high temperature peanut meal as raw material, it is pulverized and sieved, get 20～150 order peanut meals, the volume ratio ratio of 1: 1～1: 6 is added the acid solution of 0.1～1.5% (W/V) by weight, then stirs under 20～100 ℃ of conditions and soaks 0.5～5h.
2, steam blasting is processed
The peanut meal remaining acid in surface after washing away step 1 and soak with clear water, the blast chamber in the steam blasting equipment of packing into after draining, abrupt release pressure after under the pressure of 1.0～2.5MPa, dimension is pressed 1～12min, collection material.
3, the molten lixiviate of alkali
With the high temperature peanut meal after step 2 explosion, take the material-water ratio of 1: 5～1: 15 be scattered in temperature as 30～80 ℃, pH as 8～12 alkali lye in stirring and leaching 0.5～6h, centrifugation goes out sediment, collects supernatant.Carry out under the same conditions the molten lixiviate of secondary alkali, again centrifugal, supernatant and front supernatant merge.
4, acid is heavy
The pH of step 3 gained supernatant is adjusted to 4～6, precipitation, centrifugal, lower sediment is peanut protein.With the pure water washing precipitation, remove the impurity such as sugar, salt.
5, drying
The pH of step 4 gained peanut protein is adjusted to neutrality, the dry peanut protein that gets.
In the present invention, to be peanut carry the byproduct of gained after oil through mechanical expression method or organic solvent lixiviation process to described high temperature peanut meal.
In the present invention, described acid is inorganic acid or organic acid, and inorganic acid is selected from sulfuric acid, hydrochloric acid, nitric acid or phosphoric acid, and organic acid is selected from acetic acid, citric acid, tartaric acid or propionic acid; Described alkali is inorganic base, is selected from NaOH, potassium hydroxide, sodium carbonate or ammonium hydroxide.
The pulse Steam explosive machine that the present invention uses is the pulse Steam explosive machine of mass selling in the market, for example the steam blasting device produced of Henan Hebi City right way heavy-duty machine factory.
Invention benefit production method of the present invention is simple, the process-cycle is short, and device efficiency is high.Compare with the heavy technique of the molten acid of traditional alkali, in the present invention in high temperature peanut meal the recovery rate of peanut protein improved 20～30%.Simultaneously, emulsibility and the foaming characteristic of protein product are also improved largely, improve respectively 100～150% and 15～25%, its value and added value have greatly been improved, for the peanut protein goods that are applicable to the aspects such as beverage, dairy products, Flour product take high temperature peanut meal as development of raw materials provide a kind of effective method, have broad application prospects.
Description of drawings
Fig. 1, steam blasting equipment drawing.The 1-steam valve; The 2-water tank; 3-explosion chamber top cover; 4-explosion cavity; 5-explosion chamber bottom; The 6-connecting rod; The 7-pneumatic means; The 8-trip bolt; The 9-recovery bin; The 10-cyclone separator; The 11-material receiving port.
The specific embodiment
The invention will be further described below in conjunction with specific embodiment:
Embodiment 1
High temperature peanut meal is pulverized and sieved, get 80 order peanut meal 1kg and put into the stainless steel enzyme reactor, add distilled water at 1: 3 by material-water ratio, add sulfuric acid to regulate concentration to 0.3% (W/V), stir under 60 ℃ of conditions and soak 2h.Wash away the peanut meal remaining acid in surface after immersion with clear water, drain and be placed in steam blasting equipment, process 5min under 1.6MPa pressure.High temperature peanut meal after explosion adds distilled water by material-water ratio at 1: 12, regulates pH to 9.5 with alkali lye, be the molten 2h of alkali under the condition of 60 ℃ in temperature, centrifugal 20min under the 8000r/min condition, the same terms secondary alkali is molten, and is centrifugal, merge supernatant, be that under 4.5 conditions, acid is heavy at pH, centrifugal 20min under the 8000r/min condition, taking precipitate, with pure washing 3 times, get peanut protein powder through freeze drying.After measured, the recovery rate of peanut protein is 52.6%, the emulsification vitality index of products obtained therefrom reaches 240.5m2/g, foaming characteristic reaches 137.0%, compare with the heavy method of the molten acid of alkali of not processing through steam blasting, the recovery rate of peanut protein, emulsification vitality index and foaming characteristic have improved respectively 25.8%, 140.9%, 18.1%.
Embodiment 2
High temperature peanut meal is pulverized and sieved, get 40 order peanut meal 1kg and put into the stainless steel enzyme reactor, add distilled water at 1: 5 by material-water ratio, add sulfuric acid to regulate concentration to 0.6% (W/V), stir under 80 ℃ of conditions and soak 1h.Wash away the peanut meal remaining acid in surface after immersion with clear water, drain and be placed in steam blasting equipment, process 3min under 2.0MPa pressure.High temperature peanut meal after explosion adds distilled water by material-water ratio at 1: 10, regulates pH to 8.5 with alkali lye, be the molten 1.5h of alkali under the condition of 70 ℃ in temperature, centrifugal 20min under the 8000r/min condition, the same terms secondary alkali is molten, and is centrifugal, merge supernatant, be that under 4.5 conditions, acid is heavy at pH, centrifugal 20min under the 8000r/min condition, taking precipitate, with pure washing 3 times, get peanut protein powder through freeze drying.After measured, the recovery rate of peanut protein is 50.8%, the emulsification vitality index of products obtained therefrom is to 207.6m2/g, foaming characteristic reaches 134.0%, compare with the heavy method of the molten acid of alkali of not processing through steam blasting, the recovery rate of peanut protein, emulsification vitality index and foaming characteristic have improved respectively 21.5%, 108.0%, 15.5%.
Claims (3)
1. a method of extracting peanut protein from high temperature peanut meal, is characterized in that take high temperature peanut meal as raw material, through immersions, steam blasting processings, the molten lixiviate of alkali, sourly sink, drying obtains peanut protein; Step is as follows:
(1) dipping pretreatment of high temperature peanut meal: take high temperature peanut meal as raw material, it is pulverized and sieved, get 20～150 order peanut meals, the volume ratio ratio of 1: 1～1: 6 is added the acid solution of 0.1～1.5% (W/V) by weight, then stirs under 20～100 ℃ of conditions and soaks 0.5～5h;
(2) steam blasting is processed: wash away the peanut meal remaining acid in surface after step (1) is soaked, the blast chamber in the steam blasting equipment of packing into after draining, abrupt release pressure after under the pressure of 1.0～2.5MPa, dimension is pressed 1～12min, collection material with clear water;
(3) the molten lixiviate of alkali: with the high temperature peanut meal after step (2) explosion, take the material-water ratio of 1: 5～1: 15 be scattered in temperature as 30～80 ℃, pH as 8～12 alkali lye in stirring and leaching 0.5～6h, centrifugation goes out sediment, collects supernatant.Carry out under the same conditions the molten lixiviate of secondary alkali, again centrifugal, supernatant and front supernatant merge;
(4) acid is heavy: the pH of step (3) gained supernatant is adjusted to 4～6, and precipitation, centrifugal, lower sediment is peanut protein.With the pure water washing precipitation, remove the impurity such as sugar, salt;
(5) drying: the pH of step (4) gained peanut protein is adjusted to neutrality, the dry peanut protein that gets.
2. method according to claim 1 is characterized in that described high temperature peanut meal is peanut and carries the byproduct of gained after oil through mechanical expression method or organic solvent lixiviation process.
3. method according to claim 1, is characterized in that described acid is inorganic acid or organic acid, and inorganic acid is selected from sulfuric acid, hydrochloric acid, nitric acid or phosphoric acid, and organic acid is selected from acetic acid, citric acid, tartaric acid or propionic acid; Described alkali is inorganic base, is selected from NaOH, potassium hydroxide, sodium carbonate or ammonium hydroxide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105451376A CN103120249A (en) | 2012-12-17 | 2012-12-17 | Method for extracting peanut protein from high-temperature peanut meals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105451376A CN103120249A (en) | 2012-12-17 | 2012-12-17 | Method for extracting peanut protein from high-temperature peanut meals |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103120249A true CN103120249A (en) | 2013-05-29 |
Family
ID=48451545
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012105451376A Pending CN103120249A (en) | 2012-12-17 | 2012-12-17 | Method for extracting peanut protein from high-temperature peanut meals |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103120249A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103750140A (en) * | 2014-01-08 | 2014-04-30 | 北京中科百瑞能工程技术有限责任公司 | Method for processing wheat and producing whole wheat flour by using steam blasting technology |
| CN107056935A (en) * | 2016-09-27 | 2017-08-18 | 济南米铎碳新能源科技有限公司 | The preparation method of metallothionein |
| CN109043255A (en) * | 2018-07-11 | 2018-12-21 | 山东省花生研究所 | A method of it synchronizes and prepares high oleic acid peanut oil and high oleic acid peanut reconstitutes drink |
| CN109354599A (en) * | 2018-11-08 | 2019-02-19 | 天津科技大学 | A kind of deliquescent method of raising high temperature peanut meal |
| CN110663930A (en) * | 2019-10-29 | 2020-01-10 | 天津科技大学 | Preparation method of burnt coffee-flavored meat-flavored essence and product thereof |
| CN116784423A (en) * | 2023-05-23 | 2023-09-22 | 山东省农业科学院 | Method for simultaneously improving starch hydrolysis rate and protein quality in hot pressed peanut meal |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050016525A1 (en) * | 2003-07-08 | 2005-01-27 | Thorre Doug Van | Grain fractionation |
| CN1631197A (en) * | 2004-12-20 | 2005-06-29 | 北京硕泰生物技术有限责任公司 | Technology for preparing concentrated soyabean protein by continuous gradient extraction |
| CN101387084A (en) * | 2007-09-10 | 2009-03-18 | 中粮集团有限公司 | Method for steam blasting cellulose-containing material |
| CN102048021A (en) * | 2010-11-15 | 2011-05-11 | 山东省花生研究所 | Method for extracting peanut protein |
| CN102154400A (en) * | 2010-12-10 | 2011-08-17 | 广西大学 | Method for preparing dietary fiber from bean dregs serving as raw material by combining steam explosion and enzymolysis |
-
2012
- 2012-12-17 CN CN2012105451376A patent/CN103120249A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050016525A1 (en) * | 2003-07-08 | 2005-01-27 | Thorre Doug Van | Grain fractionation |
| CN1631197A (en) * | 2004-12-20 | 2005-06-29 | 北京硕泰生物技术有限责任公司 | Technology for preparing concentrated soyabean protein by continuous gradient extraction |
| CN101387084A (en) * | 2007-09-10 | 2009-03-18 | 中粮集团有限公司 | Method for steam blasting cellulose-containing material |
| CN102048021A (en) * | 2010-11-15 | 2011-05-11 | 山东省花生研究所 | Method for extracting peanut protein |
| CN102154400A (en) * | 2010-12-10 | 2011-08-17 | 广西大学 | Method for preparing dietary fiber from bean dregs serving as raw material by combining steam explosion and enzymolysis |
Non-Patent Citations (7)
| Title |
|---|
| K. WANG 等: "Influence of steaming pressure on steam explosion pretreatment of Lespedeza stalks (Lespedeza crytobotrya) Part 1. Characteristics of degraded cellulose", 《POLYMER DEGRADATION AND STABILITY》 * |
| WEI ZHAO 等: "Sustainable and practical utilization of feather keratin by an innovative physicochemical pretreatment: high density steam flash-explosion", 《GREEN CHEMISTRY》 * |
| 张益奇 等: "采用汽爆技术提高羽毛角蛋白生物效价的研究", 《食品与机械》 * |
| 曲刚 等: "从高温粕和低温粕中提取的分离蛋白的性质比较", 《食品工业科技》 * |
| 杨伟强 等: "碱提酸沉法制取花生分离蛋白工艺研究", 《花生学报》 * |
| 高丽霄 等: "高温花生粕中花生蛋白提取工艺研究", 《食品工业科技》 * |
| 高云中 等: "高温花生粕蛋白提取及功能性质的研究", 《食品与发酵工业》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103750140A (en) * | 2014-01-08 | 2014-04-30 | 北京中科百瑞能工程技术有限责任公司 | Method for processing wheat and producing whole wheat flour by using steam blasting technology |
| CN107056935A (en) * | 2016-09-27 | 2017-08-18 | 济南米铎碳新能源科技有限公司 | The preparation method of metallothionein |
| CN109043255A (en) * | 2018-07-11 | 2018-12-21 | 山东省花生研究所 | A method of it synchronizes and prepares high oleic acid peanut oil and high oleic acid peanut reconstitutes drink |
| CN109354599A (en) * | 2018-11-08 | 2019-02-19 | 天津科技大学 | A kind of deliquescent method of raising high temperature peanut meal |
| CN109354599B (en) * | 2018-11-08 | 2022-04-19 | 天津科技大学 | Method for improving solubility of high-temperature peanut meal |
| CN110663930A (en) * | 2019-10-29 | 2020-01-10 | 天津科技大学 | Preparation method of burnt coffee-flavored meat-flavored essence and product thereof |
| CN110663930B (en) * | 2019-10-29 | 2022-11-08 | 天津科技大学 | Preparation method of burnt coffee-flavored meat flavor and product thereof |
| CN116784423A (en) * | 2023-05-23 | 2023-09-22 | 山东省农业科学院 | Method for simultaneously improving starch hydrolysis rate and protein quality in hot pressed peanut meal |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103120249A (en) | Method for extracting peanut protein from high-temperature peanut meals | |
| CN102302083B (en) | Method for extracting tea protein product | |
| CN101224022B (en) | Processing method for simultaneously preparing sesame oil and protein by water enzymatic extraction | |
| CN102212976B (en) | Method for separating bagasse cellulose from lignin | |
| CN106011199B (en) | Pretreatment method of crop straws | |
| Fernandes et al. | Bioethanol production from extracted olive pomace: dilute acid hydrolysis | |
| CN108003251B (en) | A kind of extracting method of pineapple bran polysaccharide | |
| CN102816632A (en) | Method for digesting grease through microwave pretreatment and aqueous enzymatic method | |
| CN102329382A (en) | Method for extracting rapeseed proteins through ultrasonic-microwave synergy | |
| CN101381754A (en) | Method for producing fermentable sugars by hydrolysis of cellulosic component sulphonation separation couple enzyme | |
| CN110256603A (en) | A kind of-two step enzyme method coupling of shrimp and crab shells hydro-thermal prepares the methods and applications of chitin and chitosan | |
| CN105542937A (en) | Method for extracting grease from soybeans through deep processing | |
| CN104313928B (en) | The method of hemicellulose in high efficiency separation straw | |
| CN104140471A (en) | Method for preparing yam high purity polysaccharide from yam food processing leftovers | |
| CN103255659A (en) | Method for processing lignocellulosic biomass at constant pressure with ammonia and diluted alkali combined | |
| CN102550659A (en) | Method for microwave pretreatment and water extraction of edible oil | |
| CN103907938A (en) | Preparation method of jujube fruit residue dietary fiber | |
| CN102994209A (en) | Method for synchronously extracting soybean oil and soybean protein through aqueous enzymatic method | |
| CN108117652A (en) | A kind of extracting method of enzymolysis xylogen | |
| CN106755249A (en) | A kind of method of degreasing high-temperature rice bran dregs of rice comprehensive utilization | |
| CN105777860A (en) | Microwave assisted process method for extracting tea residue protein | |
| CN102296099A (en) | Method for extracting protein and phytic acid from rapeseed cake | |
| CN101263217A (en) | Process of plant oil, crude protein and fiber by water extraction | |
| CN106397611A (en) | Separation method for two-step-organic-solvent biological-refined ingredients of oil tea processing wastes | |
| CN103570794A (en) | Extracting method of camellia seed saponin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130529 |