CN106755249A - A kind of method of degreasing high-temperature rice bran dregs of rice comprehensive utilization - Google Patents
A kind of method of degreasing high-temperature rice bran dregs of rice comprehensive utilization Download PDFInfo
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Abstract
The invention discloses a kind of method to rice bran meal comprehensive utilization.The method includes:Raw material is after pretreatment, liquefaction, acidleach carries phytic acid, then separation of solid and liquid is carried out, solid portion obtains rice bran protein through enzymatic treatment, and supernatant fraction is heavy through alkali, further separation of solid and liquid, liquefied starch and phytate are respectively obtained, said two products through being saccharified and refining, obtain glucose and phytic acid product respectively.The method according to the invention can extract glucose in rice bran meal, phytic acid, albumen and cellulose products, and extraction process rationally, can take into account the recovery rate of each component, and comprehensive utilization ratio is high, with industrial application value higher.
Description
Technical field
The invention belongs to the technical field of paddy byproduct comprehensive utilization, more specifically to a kind of comprehensive to rice bran meal
The method that conjunction is utilized.
Background technology
China is paddy producing country maximum in the world, and paddy total output accounts for 1/3rd left sides of world's paddy total output
It is right.Rice bran is the accessory substance of generation during paddy rice rice milling, is a kind of renewable resource having a large capacity and a wide range, wherein rich in many hatching eggs
White matter, starch, fat, phytic acid, vitamin, cellulose etc..At present in China's rice bran except extracting rice bran oil, other are used as at a low price
The feed of value even waste material, the wasting of resources is than more serious.
There is comprehensive utilization of the more research for rice bran or defatted rice bran, such as patent CN201210081904 is carried
A kind of method for comprehensively utilizing defatted rice bran is arrived, the starch in rice bran has been extracted respectively, phytic acid, albumen and fiber etc. have been produced
Product.In extraction process, the method extracts the starch in raw material, and further liquefying-saccharifying first, is then extracted from residue
Other products such as phytic acid.Although its comprehensive utilization ratio is higher, because phytic acid can partly exist in solution in acid condition,
Therefore the technique inevitably causes phytic acid to lose.Patent CN201110004840.1 refer to a kind of comprehensive utilization rice bran
Method, while extracting the rice bran oil in rice bran, rice bran crude fibre, rice bran protein, and Rice Bran Polypeptides product, the technique has abandoned plant
Acid and two kinds of products of starch, comprehensive utilization ratio is not relatively high.
Patent CN201310413681.X refer to a kind of method of comprehensive utilization of defatted rice bran, the method achieve degreasing
The comprehensive utilization of rice bran, produces the phytic acid calcium with better quality, crystal glucose, rice bran protein polypeptide and rice bran-fiber four
Product is planted, the actual application value of defatted rice bran is improve, with extraordinary popularizing application prospect.But mentioned in the method
In technique, during first step acidleach is carried, substantial amounts of starch can be lost, not only result in larger waste, and starch presence
Plate-frame filtering can be caused difficult.In addition, sour molten and alkali sinks repeatedly during phytic acid calcium is obtained, a large amount of soda acids, resource are consumed
It is larger with environmental protection pressure.
Patent CN201110200584.3 refer to a kind of method of comprehensive utilization of rice bran meal, and the method is entered including rice bran meal
Row liquefaction, liquid phase carries out anaerobic fermentation, and solid phase is through enzymolysis, concentration, the dry raw material as production feed.The method extraction and application
Starch (being changed into glucose) and phytic acid (being changed into inositol) in rice bran meal, but without the egg in extraction and application rice bran meal
In vain, and starch isolation utilizes process, phytic acid loss late is larger.It is as production feedstuff that other final products still have part
Use, added value is not high.
In above-mentioned existing production technology, in order to prevent the grease oxidation rancid in rice bran, rice bran from all have passed through thermally-stabilised
Change is processed, and albumen considerable part there occurs the cohesion after denaturation in the rice bran meal for finally giving, and actual soluble protein ratio is big
It is big to reduce.It is relatively rational more simultaneous the invention provides a kind of method of degreasing high-temperature rice bran dregs of rice comprehensive utilization for this feature
The recovery rate of each component is turned round and look at.
The content of the invention
For the problem that above-mentioned prior art is present, it is an object of the present invention to provide a kind of degreasing high-temperature rice bran dregs of rice
The method of comprehensive utilization, the starch that can be extracted in rice bran meal of the invention, phytic acid, albumen and fiber product, extraction process is reasonable,
The recovery rate of each component can be taken into account, comprehensive utilization ratio is high, with industrial application value higher.
The method of degreasing high-temperature rice bran dregs of rice comprehensive utilization according to the present invention, the method is comprised the following steps:
(1) pretreatment of raw material:Raw rice bran is crushed, 20 mesh to 80 mesh sieves, preferably 50 mesh sieves are crossed;
(2) product obtained in step (1), is 1 by feed liquid weight ratio:4 to 1:10 addition water, preferably 1:6, stirring is equal
It is even;
(3) material liquid pH of regulating step (2) is 2 to 5.5, preferably 3.5, and temperature is 20 to 70 degree, and preferably 50 degree, addition is pre-
Treatment enzyme, insulation pretreatment 0.5 to 3h;
(4) feed liquid of step (3) need not do any treatment, and it is 6.0 to 8.0, preferably 6.0 directly to adjust its pH, and temperature is
60 to 100 degree, addition α-amylase TSC insulations liquefaction 2 to 8 hours, are subsequently adding calcium chloride, continue to be incubated about 1 to 2 hour;
(5) according to the enzyme for being used, the material liquid pH and temperature of regulating step (4);
(6) feed liquid that step (5) is obtained is carried out into separation of solid and liquid, plate-frame filtering or centrifugation, filtrate or centrifugation can be used
Clear liquid merges;
(7) liquid obtained in step (6), first with the Ca (OH) of saturation2Solution regulation pH is 8-12, preferably 9, stirring 30
Minute, precipitation is formed, and separation of solid and liquid is carried out, can be using centrifugation or plate-frame filtering;
(8) liquid obtained in step (7), watery hydrochloric acid regulation pH to 3 to 6, preferably 4.5, temperature be 30 to 70 degree, it is excellent
Elect 45 to 65 degree as, more preferably 60 degree, add carbohydrase, addition is the minimum for reaching requirement, is saccharified 48 hours,
Obtain glucose solution;
(9) solid obtained in step (7), add watery hydrochloric acid regulation pH to 2-6, preferably 3.5, to dissolving all solids,
Filtering, filtrate is refined into cationic ion-exchange resin, finally gives plant acid solution;
(10) solid that will be obtained in step (6), is 1 with feed liquid weight ratio:5 to 1:20 addition water, preferably 1:5 to 1:
15, more preferably 1:10, crushed into colloid mill, pH value and temperature are adjusted, after adding various ferment treatments, through cyclonic separation, obtain
To albumen and cellulose products.
Preferably, step 3) described in pretreatment enzyme can be selected from Viscozyme, complex cellulase and endoglucanase
One or more in enzyme, the consumption of the pretreatment enzyme is 0.1 ‰ to 10 ‰, preferably the 0.5 ‰ to 2 ‰ of raw material.
The method according to the invention, glucose yield is about more than 27%, and phytic acid yield is about more than 6.5%, rice bran egg
White yield is about more than 70%, and cellulose yield is about more than 15%.
Beneficial effect
Preparation in accordance with the present invention process is simple, extraction process rationally, can take into account the recovery rate of each component, comprehensive
Utilization rate is high, and a large amount of soda acids are not introduced into processing procedure, economic and environment-friendly, with industrial application value higher.In addition, a kind of group
The separation for dividing can reduce the cost of subsequent component extraction, improve the purity of subsequent component, and the present invention takes full advantage of living resources,
Bigger economic benefit can be produced.
Specific embodiment
By the raw material and the feed liquid weight of water after step (1) sieving in step (2) in method according to the present invention
Than being 1:4 to 1:10, preferably 1:6.When feed liquid weight ratio is less than 1:When 4, i.e. amount of water are not enough, then material concentration is excessive, causes
React not abundant enough in subsequent reactions step;When feed liquid weight ratio is more than 1:10, i.e., when amount of water is excessive, then cause the wave of water
Take, while in order to strengthen the reaction of raw material in subsequent step, and need to add the reagents such as more enzymes, economy is not good.
Material liquid pH and temperature in step (2) can be adjusted according to the pretreatment enzyme for using.For example when using compound
During cellulase, pH is about 6, and temperature control is 55 degree, insulation pretreatment 1 hour, and when using endoglucanase, pH is about
7, temperature control is 70 degree, insulation pretreatment 1 hour.Preferably, the material liquid pH is adjusted to 2 to 5.5, preferably 3.5, and temperature is
20 to 70 degree, preferably 50 degree, insulation pretreatment 0.5 to 3h.
Preferably, step 3) described in pretreatment enzyme can be selected from Viscozyme, complex cellulase and endoglucanase
One or more in enzyme, the consumption of the pretreatment enzyme is 0.1 ‰ to 10 ‰, preferably the 0.5 ‰ to 2 ‰ of raw material.If
The consumption of the pretreatment enzyme is less than 0.1 ‰, then the consumption for pre-processing enzyme is not enough, causes the reaction time long, and after being unfavorable for
Continuous reactions steps;If the consumption of the pretreatment enzyme is more than 10 ‰, the consumption for pre-processing enzyme is excessive, causes reaction speed
It is too fast and whard to control, and cost greatly increases, it is not economical enough.
Hereinafter, will be described in detail the present invention.Before doing so, it should be appreciated that in this specification and appended
Claims in the term that uses should not be construed as and be limited to general sense and dictionary meanings, and inventor should allowed
Term is suitably defined with the basis of the principle for carrying out best interpretations, according to implication corresponding with technical elements of the invention and generally
Thought is explained.Therefore, description presented herein is not intended to limitation originally merely for the sake of the preferred embodiment for illustrating purpose
The scope of invention, it will thus be appreciated that without departing from the spirit and scope of the present invention, it can be obtained by it
His equivalents or improved procedure.
Following examples are enumerated only as the example of embodiment of the present invention, do not constitute any limit to the present invention
System, it will be appreciated by those skilled in the art that the modification in the range of without departing from essence of the invention and design each falls within the present invention
Protection domain.Unless stated otherwise, the reagent and instrument for being used in following examples are commercially available product.
Embodiment 1:
(1) pretreatment of raw material:Degreasing high-temperature rice bran dregs of rice raw material is crossed into 50 mesh sieves;
(2) raw material 30g under the sieve obtained in step (1) is taken, by solid-liquid ratio 1:4 addition water, stir;
(3) the feed liquid condition pH=3.5 of regulating step (2), 50 degree of temperature, addition pretreatment enzyme Viscozyme 150ul,
Insulation pretreatment 1h;
(4) material liquid pH=6 of regulating step (3), temperature is 90 degree, adds the CaCl of 0.1mol/L210ml, annex solution
Change enzyme TSC 300ul, liquefy 1h, and iodine examination is qualified, and wherein TSC is a kind of liquid enzyme formulation, containing the extremely strong α of heat endurance-
Amylase, is expressed using the rod bacterium (Bacillus) of genetic modification and is made up of base.The specific name of enzyme is Isosorbide-5-Nitrae-α-D types Portugal
Endohydrolase (EC3.2.1.1), is commercially available prod;
(5) material liquid pH of regulating step (4) is 2.5, and temperature is 40 degree, is incubated 2 hours;
(6) feed liquid that step (5) is obtained is carried out into suction filtration, and respectively with 3 parts of washings twice, filtrate mixing.
(7) liquid obtained in step (6), first with Ca (OH)2PH=6 is adjusted to, then pH=8, stirring 30 are adjusted to NaOH
Minute, precipitation is formed, filter;
(8) mixing liquid obtained in step (7), adjusts pH4.5, temperature 60, addition carbohydrase (0.03%), saccharification
48h, finally gives glucose product;
(9) solid obtained in step (7), after acid adding dissolving, using 0.01 micron of ultrafiltration membrance filter, hands over into anion
Resin 717 is changed, cationic ion-exchange resin 732 (resin is purchased from Tianjin Nankai Compositech Inc.) is entered after wash-out, most
Phytic acid product is obtained eventually;
(10) solid that will be obtained in step (6), by solid-liquid ratio 1:10 addition water, adjust pH value 6, add Cellucalst
Enzyme, after processing 3 hours, adjusts pH3.5, and 50 degree of temperature adds viscozyme ferment treatments one hour, and regulation pH is 8, and temperature is
55 degree, addition basic protein ferment treatment 2 hours, cyclonic separation obtains two kinds of products of albumen and cellulose.
Final glucose yield is 26.3%, and phytic acid yield is 6%, and rice bran protein yield is 43.3%, cellulose yield
It is 17.3%.
Embodiment 2:
(1) pretreatment of raw material:Degreasing high-temperature rice bran dregs of rice raw material is crossed into 50 mesh sieves;
(2) raw material 30g under the sieve obtained in step (1) is taken, by solid-liquid ratio 1:4 addition water, stir;
(3) the feed liquid condition pH=3.5 of regulating step (2), 50 degree of temperature, addition pretreatment enzyme Viscozyme 150ul,
Insulation pretreatment 1h;
(4) material liquid pH=6 of regulating step (3), temperature is 90 degree, adds the CaCl of 0.1mol/L210ml, annex solution
Change enzyme TSC 300ul, liquefy 1h, and iodine examination is qualified;
(5) material liquid pH of regulating step (4) is 2.5, and temperature is 40 degree, is incubated 2 hours;
(6) feed liquid that step (5) is obtained is carried out into suction filtration, and respectively with 3 parts of washings twice, filtrate mixing.
(7) liquid obtained in step (6), pH=8 is adjusted to NaOH, is stirred 30 minutes, forms precipitation, is filtered;
(8) mixing liquid obtained in step (7), adjusts pH4.5, temperature 60, addition carbohydrase (0.03%), saccharification
48h, finally gives glucose product;
(9) solid obtained in step (7), after acid adding dissolving, using 0.01 micron of ultrafiltration membrance filter, hands over into anion
Resin 717 is changed, cationic ion-exchange resin 732 is entered after wash-out, finally give phytic acid product;
(10) solid that will be obtained in step (6), by solid-liquid ratio 1:10 addition water, (are implemented for 1 hour using milling treatment of colloid
Without this step in example 1), pH value 6 is adjusted, Cellucalst enzymes are added, after processing 3 hours, adjust pH3.5,50 degree of temperature, addition
Viscozyme ferment treatments one hour, regulation pH is 8, and temperature is 55 degree, addition basic protein ferment treatment 2 hours, cyclonic separation,
Obtain two kinds of products of albumen and cellulose.
Final glucose yield is 26%, and phytic acid yield is 5.3%, and rice bran protein yield is 26.6%, and fiber is
19.3%.
Claims (5)
1. a kind of method that degreasing high-temperature rice bran dregs of rice are comprehensively utilized, the method is comprised the following steps:
(1) pretreatment of raw material:Raw rice bran is crushed, 20 mesh to 80 mesh sieves are crossed;
(2) product obtained in step (1), is 1 by feed liquid weight ratio:4 to 1:10 addition water, stir;
(3) material liquid pH of regulating step (2) is 2 to 5.5, and temperature is 20 to 70 degree, addition pretreatment enzyme, insulation pretreatment 0.5
To 3h;
(4) feed liquid of step (3) need not do any treatment, and it is 6.0 to 8.0 directly to adjust its pH, and temperature is 60 to 100 degree, is added
Plus α-amylase TSC insulations are liquefied 2 to 8 hours, are subsequently adding calcium chloride, continue to be incubated about 1 to 2 hour;
(5) according to the enzyme for being used, the material liquid pH and temperature of regulating step (4);
(6) feed liquid that step (5) is obtained is carried out into separation of solid and liquid, plate-frame filtering or centrifugation, filtrate or centrifugal clear liquid can be used
Merge;
(7) liquid obtained in step (6), first with the Ca (OH) of saturation2Solution regulation pH is 8-12, is stirred 30 minutes, forms heavy
Form sediment, and carry out separation of solid and liquid, can be using centrifugation or plate-frame filtering;
(8) liquid obtained in step (7), watery hydrochloric acid regulation pH to 3 to 6, temperature is 30 to 70 degree, adds carbohydrase, addition
Measure to reach the minimum of requirement, be saccharified 48 hours, obtain glucose solution;
(9) solid obtained in step (7), adds watery hydrochloric acid to adjust pH to 2-6, and to dissolving all solids, filtering, filtrate enters
Cationic ion-exchange resin is refined, and finally gives plant acid solution;
(10) solid that will be obtained in step (6), is 1 with feed liquid weight ratio:5 to 1:20 addition water, crush into colloid mill, adjust
Section pH value and temperature, after adding various ferment treatments, through cyclonic separation, obtain albumen and cellulose products.
2. the method that the degreasing high-temperature rice bran dregs of rice according to claim 1 are comprehensively utilized, it is characterised in that
Raw rice bran crushed 50 mesh sieves in the step (1);
It is 1 that feed liquid weight ratio is pressed in step (2):6 addition water, stir;
The material liquid pH of regulating step (2) is 3.5 in step (3), and temperature is 50 degree;
It is 6.0 that the feed liquid of step (3) directly adjusts its pH in step (4);
The Ca (OH) of step (7) middle saturation2Solution regulation pH is 9;
It is 4.5 to adjust pH with watery hydrochloric acid in step (8), and temperature is 45 to 65 degree, more preferably 60 degree;
Watery hydrochloric acid regulation pH to 3.5 is added in step (9);
With feed liquid weight ratio it is 1 in step (10):5 to 1:15 addition water, more preferably 1:10.
3. the method that the degreasing high-temperature rice bran dregs of rice according to claim 1 are comprehensively utilized, it is characterised in that in the step 3)
Described in pretreatment enzyme be selected from Viscozyme, complex cellulase and endoglucanase in one or more, the pre- place
It is the 0.1 ‰ to 10 ‰ of raw material to manage the consumption of enzyme.
4. the method that the degreasing high-temperature rice bran dregs of rice according to claim 1 are comprehensively utilized, it is characterised in that in the step 3)
Described in pretreatment enzyme consumption for raw material 0.5 ‰ to 2 ‰.
5. the method that the degreasing high-temperature rice bran dregs of rice as claimed in any of claims 1 to 4 are comprehensively utilized, its feature exists
According to methods described, glucose yield is about more than 27%, and phytic acid yield is about more than 6.5%, and rice bran protein yield is about
More than 70%, cellulose yield is about more than 15%.
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CN107098928A (en) * | 2017-06-20 | 2017-08-29 | 中国科学院青岛生物能源与过程研究所 | The method that phytic acid is extracted |
CN107691763A (en) * | 2017-09-19 | 2018-02-16 | 中国科学院青岛生物能源与过程研究所 | A kind of method of comprehensive utilization of wheat bran |
CN111374263A (en) * | 2018-12-27 | 2020-07-07 | 丰益(上海)生物技术研发中心有限公司 | Aqueous rice bran extract and its preparation method and application |
CN113367336A (en) * | 2020-03-10 | 2021-09-10 | 丰益(上海)生物技术研发中心有限公司 | Method for processing rice bran |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107098928A (en) * | 2017-06-20 | 2017-08-29 | 中国科学院青岛生物能源与过程研究所 | The method that phytic acid is extracted |
CN107691763A (en) * | 2017-09-19 | 2018-02-16 | 中国科学院青岛生物能源与过程研究所 | A kind of method of comprehensive utilization of wheat bran |
CN111374263A (en) * | 2018-12-27 | 2020-07-07 | 丰益(上海)生物技术研发中心有限公司 | Aqueous rice bran extract and its preparation method and application |
CN111374263B (en) * | 2018-12-27 | 2023-06-23 | 丰益(上海)生物技术研发中心有限公司 | Rice bran aqueous solution extract, preparation method and application thereof |
CN113367336A (en) * | 2020-03-10 | 2021-09-10 | 丰益(上海)生物技术研发中心有限公司 | Method for processing rice bran |
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