CN107691763A - A kind of method of comprehensive utilization of wheat bran - Google Patents
A kind of method of comprehensive utilization of wheat bran Download PDFInfo
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- CN107691763A CN107691763A CN201710847539.4A CN201710847539A CN107691763A CN 107691763 A CN107691763 A CN 107691763A CN 201710847539 A CN201710847539 A CN 201710847539A CN 107691763 A CN107691763 A CN 107691763A
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- wheat bran
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- 235000015099 wheat brans Nutrition 0.000 title claims abstract description 61
- 238000000034 method Methods 0.000 title claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 53
- 102000004190 Enzymes Human genes 0.000 claims abstract description 35
- 108090000790 Enzymes Proteins 0.000 claims abstract description 35
- 229940088598 enzyme Drugs 0.000 claims abstract description 35
- 239000002994 raw material Substances 0.000 claims abstract description 23
- 239000000463 material Substances 0.000 claims abstract description 22
- 239000007787 solid Substances 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000000047 product Substances 0.000 claims abstract description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 11
- 239000008103 glucose Substances 0.000 claims abstract description 11
- 238000000926 separation method Methods 0.000 claims abstract description 10
- 238000009413 insulation Methods 0.000 claims abstract description 9
- 229920002678 cellulose Polymers 0.000 claims abstract description 8
- 239000001913 cellulose Substances 0.000 claims abstract description 8
- 239000002253 acid Substances 0.000 claims abstract description 7
- 238000001556 precipitation Methods 0.000 claims abstract description 6
- 239000001110 calcium chloride Substances 0.000 claims abstract description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 4
- 239000000084 colloidal system Substances 0.000 claims abstract description 4
- 241000196324 Embryophyta Species 0.000 claims abstract description 3
- 102000004139 alpha-Amylases Human genes 0.000 claims abstract description 3
- 108090000637 alpha-Amylases Proteins 0.000 claims abstract description 3
- 229940024171 alpha-amylase Drugs 0.000 claims abstract description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 12
- 230000001105 regulatory effect Effects 0.000 claims description 10
- 108010059892 Cellulase Proteins 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 108091005804 Peptidases Proteins 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 5
- 239000003729 cation exchange resin Substances 0.000 claims description 4
- 229940106157 cellulase Drugs 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims 1
- 238000007781 pre-processing Methods 0.000 claims 1
- 241000209140 Triticum Species 0.000 abstract description 5
- 235000021307 Triticum Nutrition 0.000 abstract description 5
- 239000006227 byproduct Substances 0.000 abstract description 4
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 8
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 8
- 229940068041 phytic acid Drugs 0.000 description 8
- 235000002949 phytic acid Nutrition 0.000 description 8
- 239000000467 phytic acid Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 239000000835 fiber Substances 0.000 description 6
- 230000008901 benefit Effects 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000003513 alkali Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 235000013325 dietary fiber Nutrition 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 239000003957 anion exchange resin Substances 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 108010089934 carbohydrase Proteins 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 150000007965 phenolic acids Chemical class 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010013554 Diverticulum Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical class [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000005422 blasting Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 208000001130 gallstones Diseases 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 229930013686 lignan Natural products 0.000 description 1
- 235000009408 lignans Nutrition 0.000 description 1
- 150000005692 lignans Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/12—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses
- A23J1/125—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses by treatment involving enzymes or microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/02—Glucose; Glucose-containing syrups obtained by saccharification of cellulosic materials
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention provides a kind of method of comprehensive utilization of wheat bran, is related to the technical field that wheat byproduct comprehensively utilizes, and this method comprises the following steps：Wheat bran raw material pulverizes and sieves, and adds water, pretreatment, the TSC insulation liquefaction of addition α-amylase, then adds CaCl2Solution, continue to be incubated 1h 2h；According to used enzyme, material liquid pH and temperature are adjusted, is reacted；Separation of solid and liquid, obtain solid 1 and liquid 1, liquid 1, with the Ca (OH) of saturation2Solution adjusts precipitation, and carries out separation of solid and liquid and obtain solid 2 and liquid 2；The saccharification of liquid 2 is taken to obtain glucose solution；Isolated plant acid solution after taking the acid of solid 2 molten；Take solid 1 plus water to be crushed into colloid mill, albumen and cellulose products are obtained after ferment treatment.The various efficiency of pcr product of this method are high, realize the comprehensive utilization to wheat bran, green.
Description
Technical field
The invention belongs to the technical field of wheat byproduct comprehensive utilization, and wheat bran is integrated more specifically to a kind of
The method utilized.
Background technology
Wheat bran is wheat processing Main By product, wherein containing abundant carbohydrate, protein, vitamin, mineral
Matter etc., it is widely used in feed industry, food industry, fermentation industry.With the continuous improvement of people's health consciousness, the work(in wheat bran
The energy factor is increasingly valued by people.The development and utilization of wheat bran, there is higher Social benefit and economic benefit.
2 major class physiological activators i.e. active polysaccharide and phenolic compound is mainly contained in wheat bran.Active polysaccharide is mainly
Refer to wheat edible fiber.Dietary fiber can be reduced reduces the absorption of glucose in the passage time of small intestine, slows down Starch Hydrolysis, right
Reducing cholesterolemia, high fat of blood, coronary heart disease, hypertension has good prevention effect；Wheat-bran dietary fiber is to relying on insulin
Diabetic's action effect it is good；Also it is reduced the effect that diverticulosis and gall stone form and prevented colon cancer；To just
It is secret also to have certain food therapy effect [1].Aldehydes matter in wheat bran mainly has phenolic acid, flavonoids and lignan, and phenolic acid has anti-
Oxidisability and antitumaous effect；Flavonoids has the effect of certain in anti-aging, prevention of cardiovascular disease, anti-cancer, anticancer aspect.
The research of wheat bran comprehensive utilization is few, and the research that wheat bran is processed into Speciality Foods is relatively more.Such as CN
201410260497.0 the grade research of CN 201410611615.8, CN 201410599432.9 is all that wheat bran is processed into wheat bran
The patent of invention of sauce.CN201210086203.8 refer to a kind of extracting method of wheat bran albumen, be by the heavy side of alkali soluble acid
Method, wheat bran albumen is obtained, recovery rate is up to 32%.CN 201410766873.3 discloses a kind of using wheat bran as raw material preparation wheat
The method of bran dietary fiber, by alkali soluble, steam blasting, composite protease hydrolysis, the methods of multiple alcohol precipitation, obtain wheat bran meals
Fiber.CN 201610588548.1 discloses the new technology that a kind of high-valued comprehensive utilization wheat bran prepares polysaccharide, the invention
Using agricultural byproducts processing accessory substance wheat bran as raw material, being prepared using microbial fermentation and biological enzymolysis technology has rush Bifidobacterium
The xylo-oligosaccharide breed, prevent constipation, reduced cholesterol, protection liver and other effects, realizes the efficient utilization of wheat bran, improves
Wheat bran is worth.To sum up, the extraction research of one-component is more, causes the utilization to wheat bran not comprehensive, causes the wasting of resources and ring
Pollute in border.
The content of the invention
In view of the above-mentioned problems of the prior art, it is an object of the present invention to provide a kind of comprehensive utilization of wheat bran
Method, the present invention can extract starch, phytic acid, albumen and fiber product in wheat bran, and extraction process is reasonable, can take into account each group
The recovery rate divided, comprehensive utilization ratio is high, has higher industrial application value.
The method of wheat bran comprehensive utilization according to the present invention, this method comprise the following steps：
(1) pretreatment of raw material：Wheat bran raw material is crushed, crosses 20-80 mesh sieves；
(2) product obtained in step (1), it is 1 by feed liquid weight ratio:4-1:10 addition water, stir；
(3) material liquid pH of regulating step (2) is 2-5.5, and regulation temperature is 20-70 DEG C, addition pretreatment enzyme, is incubated pre- place
Manage 0.5h-3h；
(4) the feed liquid regulation pH of step (3) be 6.0-8.0, and temperature is 60-100 DEG C, and adding α-amylase TSC, (addition is
‰) the 0.5 ‰ of wheat bran material quality, insulation liquefaction 2h-8h, then add CaCl to 102(addition is wheat bran material quality
0.5 ‰ to 10 ‰), continues to be incubated 1h-2h；
(5) enzyme, material liquid pH and temperature (the pH 6.0-8.0, temperature 60-100 of regulating step (4) used in
DEG C), reacted；
(6) feed liquid for obtaining step (5) carries out separation of solid and liquid；
(7) liquid obtained in step (6) is taken, first with the Ca (OH) of saturation2Solution regulation pH is 8-12, stirs 30 points
Clock, precipitation is formed, and carry out separation of solid and liquid；
(8) liquid obtained in step (7) is taken, adjusts pH to 3-6 with watery hydrochloric acid, regulation temperature is 30-70 DEG C, addition sugar
Change enzyme, (addition is the 0.5 ‰ to 10 ‰ of wheat bran material quality), is saccharified 48 hours, obtains glucose solution；
(9) solid obtained in step (7), watery hydrochloric acid regulation pH to 2-6 is added, to all solids are dissolved, is filtered, filtrate
Refined into cationic ion-exchange resin, finally give plant acid solution；
(10) solid that will be obtained in step (6), using feed liquid weight ratio as 1:5-1:20 addition water, are milled into colloid
It is broken, pH value and temperature are adjusted, progressively addition pretreatment enzyme, Protease Treatment, processing enters cyclone separator after terminating and separated, point
Albumen and cellulose products are not obtained.
Preferably, step (1) sieving was 50 mesh sieves.
Preferably, step (2) the feed liquid weight ratio is 1:6.
Preferably, step (3) described pH is 3.5, and temperature is 50 DEG C, and the pretreatment enzyme is Viscozyme, composite fibre
One or more in plain enzyme and endoglucanase, the dosage of the pretreatment enzyme is wheat bran material quality in step (2)
0.5 ‰ to 10 ‰.
Preferably, the dosage of step (3) the pretreatment enzyme is 1 ‰ to 5 ‰ of wheat bran material quality in step (2).
Preferably, step (4) described pH is 6.0.
The separation of solid and liquid, can use plate-frame filtering or centrifugation, and filtrate or centrifugal clear liquid merge；
The method according to the invention, glucose yield are about more than 27%, and phytic acid yield is about more than 6.5%, wheat bran egg
White yield is about more than 70%, and cellulose yield is about more than 15%.
Beneficial effect
Preparation in accordance with the present invention technique is simple, and extraction process is reasonable, can take into account the recovery rate of each component, comprehensive
Utilization rate is high, and a large amount of soda acids are not introduced into processing procedure, economic and environment-friendly, has higher industrial application value.In addition, a kind of group
The separation divided can reduce the cost of subsequent component extraction, improve the purity of subsequent component, and the present invention takes full advantage of living resources,
Bigger economic benefit can be produced.
Embodiment
The feed liquid weight of raw material and water in method according to the present invention in step (2) after step (1) sieving
Than for 1:4 to 1:10, preferably 1:6.When feed liquid weight ratio is less than 1:4, i.e., amount of water deficiency when, then material concentration is excessive, causes
React not abundant enough in subsequent reactions step；When feed liquid weight ratio is less than 1:10, i.e., when amount of water is excessive, then cause the wave of water
Take, while in order to strengthen the reaction of raw material in subsequent step, and need to add the reagents such as more enzymes, economy is bad.
Material liquid pH and temperature in step (2) can be adjusted according to the pretreatment enzyme used.Such as when using compound
During cellulase, pH is about 6, and temperature control is 55 degree, and insulation pretreatment 1 hour, when using endoglucanase, pH is about
7, temperature control is 70 degree, insulation pretreatment 1 hour.Preferably, the material liquid pH is adjusted to 2 to 5.5, preferably 3.5, and temperature is
20 to 70 degree, preferably 50 degree, insulation pretreatment 0.5 to 3h.
Preferably, enzyme is pre-processed described in step (3) to be selected from Viscozyme, complex cellulase and glucan
One or more in enzyme cutting, the dosage of the pretreatment enzyme is the 0.1 ‰ to 10 ‰ of raw material.If the use of the pretreatment enzyme
Amount is less than 0.1 ‰, then pre-processes the dosage deficiency of enzyme, cause the reaction time long, and be unfavorable for follow-up reactions steps；If
The dosage of the pretreatment enzyme is more than 10 ‰, then the dosage of pretreatment enzyme is excessive, causes reaction speed too fast and whard to control, and
Cost greatly increases, not economical enough.
Embodiment 1：
(1) pretreatment of raw material：Wheat bran is crossed into 50 mesh sieves；
(2) raw material 30g under the sieve obtained in step (1) is taken, by solid-liquid ratio 1:4 addition water, stir；
(3) the feed liquid condition pH=3.5 of regulating step (2), 50 degree of temperature, addition pretreatment enzyme Viscozyme 150ul,
Insulation pretreatment 1h；
(4) material liquid pH=6 of regulating step (3), temperature are 90 degree, add 0.1mol/L CaCl210ml, annex solution
Change enzyme TSC 300ul, liquefy 1h, and iodine examination is qualified, and wherein TSC is a kind of liquid enzyme formulation, containing the extremely strong α of heat endurance-
Amylase, expressed using the rod bacterium (Bacillus) of genetic modification and be made up of base.The specific name of enzyme is Isosorbide-5-Nitrae-α-D types Portugal
Endohydrolase (EC3.2.1.1), it is commercially available prod；
(5) material liquid pH of regulating step (4) is 2.5, and temperature is 40 degree, is incubated 2 hours；
(6) feed liquid that step (5) obtains is filtered, and respectively washed twice with 3 parts, filtrate mixing.
(7) liquid obtained in step (6), first with Ca (OH)2PH=8 is adjusted to, is stirred 30 minutes, precipitation is formed, filters；
(8) mixing liquid obtained in step (7), pH4.5 is adjusted, temperature 60 C, (0.03wt% is with original for addition carbohydrase
On the basis of material wheat bran), be saccharified 48h, finally gives glucose product；
(9) solid obtained in step (7), after acid adding (2mol/L hydrochloric acid) dissolving, 0.01 micron of milipore filter mistake is utilized
Filter, into anion exchange resin 717, cationic ion-exchange resin 732 is entered after elution, and (resin is purchased from Tianjin Nankai synthesis science and technology
Co., Ltd), finally give phytic acid product；
(10) solid that will be obtained in step (6), by feed liquid mass ratio 1:10 addition water, adjust pH value 6, addition
Cellucalst enzymes (0.03wt% is on the basis of raw material wheat bran), after handling 3 hours, adjust pH3.5, temperature 50 C, addition
Viscozyme enzymes (0.03wt% is on the basis of raw material wheat bran) are handled 1 hour, and regulation pH is 8, and temperature is 55 DEG C, addition alkalescence
Protease (0.03%wt is on the basis of raw material wheat bran) is handled 2 hours, cyclonic separation, obtains two kinds of products of albumen and cellulose.
Finally give glucose 7.9g, phytic acid 1.8g, wheat bran protein 12 .9g, cellulose 5.2g；Glucose yield is
26.3%, phytic acid yield is 6%, and wheat bran albumen yield is 43.3%, and cellulose yield is 17.3%.
Embodiment 2：
(1) pretreatment of raw material：Wheat bran raw material is crossed into 50 mesh sieves；
(2) raw material 30g under the sieve obtained in step (1) is taken, by feed liquid mass ratio 1:4 addition water, stir；
(3) the feed liquid condition pH=3.5 of regulating step (2), temperature 50 C, addition pretreatment enzyme Viscozyme 150ul,
Insulation pretreatment 1h；
(4) material liquid pH=6 of regulating step (3), temperature are 90 DEG C, add 0.1mol/L CaCl210ml, annex solution
Change enzyme TSC 300ul, liquefy 1h, and iodine examination is qualified；
(5) material liquid pH of regulating step (4) is 2.5, and temperature is 40 DEG C, is incubated 2 hours；
(6) feed liquid that step (5) obtains is filtered, and respectively washed twice with 3 parts, filtrate mixing.
(7) liquid obtained in step (6), with Ca (OH)2PH=8 is adjusted to, is stirred 30 minutes, precipitation is formed, filters；
(8) mixing liquid obtained in step (7), pH4.5 is adjusted, temperature 60 C, (0.03% with raw material for addition carbohydrase
On the basis of wheat bran), be saccharified 48h, finally gives glucose product；
(9) solid obtained in step (7), after acid adding (2mol/L hydrochloric acid) dissolving, 0.01 micron of milipore filter mistake is utilized
Filter, into anion exchange resin 717, enters cationic ion-exchange resin 732, finally gives phytic acid product after elution；
(10) solid that will be obtained in step (6), by feed liquid mass ratio 1:10 addition water, using milling treatment of colloid 1 hour,
PH value 6 is adjusted, addition Cellucalst enzymes (0.03wt% is on the basis of raw material wheat bran), after handling 3 hours, adjusts pH3.5, temperature
50 DEG C of degree, addition viscozyme enzymes (0.03wt% is on the basis of raw material wheat bran) are handled one hour, and regulation pH is 8, temperature 55
DEG C, addition alkali protease (0.03wt% is on the basis of raw material wheat bran) is handled 2 hours, cyclonic separation, obtains albumen and fiber
Plain two kinds of products.
Finally give glucose 7.8g, phytic acid 1.6g, wheat bran albumen 7.98g, cellulose 5.8g；Glucose yield is
26%, phytic acid yield is 5.3%, and wheat bran albumen yield is 26.6%, fiber 19.3%.
Claims (6)
- A kind of 1. method of comprehensive utilization of wheat bran, it is characterised in that：Comprise the following steps：(1) pretreatment of raw material：Wheat bran raw material is crushed, crosses 20-80 mesh sieves；(2) product obtained in step (1), it is 1 by feed liquid weight ratio:4-1:10 addition water, stir；(3) material liquid pH of regulating step (2) is 2-5.5, and regulation temperature is 20-70 DEG C, addition pretreatment enzyme, insulation pretreatment 0.5h-3h；(4) the feed liquid regulation pH of step (3) is 6.0-8.0, and temperature is 60-100 DEG C, adds α-amylase TSC, addition is wheat bran The 0.5 ‰ to 10 ‰ of material quality, insulation liquefaction 2h-8h, then add CaCl2, addition is the 0.5 ‰ of raw material wheat bran quality To 10 ‰, continue to be incubated 1h-2h；(5) enzyme used in, the material liquid pH of regulating step (4) is 6.0-8.0, and temperature is 60-100 DEG C), reacted；(6) feed liquid for obtaining step (5) carries out separation of solid and liquid；(7) liquid obtained in step (6) is taken, first with the Ca (OH) of saturation2Solution regulation pH is 8-12, stirs 30 minutes, is formed Precipitation, and carry out separation of solid and liquid；(8) liquid obtained in step (7) is taken, adjusts pH to 3-6 with watery hydrochloric acid, regulation temperature is 30-70 DEG C, addition saccharification Enzyme, addition are the 0.5 ‰ to 10 ‰ of wheat bran material quality, are saccharified 48 hours, obtain glucose solution；(9) solid obtained in step (7), watery hydrochloric acid regulation pH to 2-6 is added, to all solids, filtering are dissolved, filtrate enters Cationic ion-exchange resin refines, and finally gives plant acid solution；(10) solid that will be obtained in step (6), using feed liquid weight ratio as 1:5-1:20 addition water, crush into colloid mill, adjust PH value and temperature being saved, progressively addition pretreatment enzyme, Protease Treatment, processing enters cyclone separator after terminating and separated, respectively To albumen and cellulose products.
- 2. the method for comprehensive utilization of wheat bran according to claim 1, it is characterised in that：Step (1) sieving was 50 mesh Sieve.
- 3. the method for comprehensive utilization of wheat bran according to claim 1, it is characterised in that：Step (2) the feed liquid weight ratio is 1:6。
- 4. the method for comprehensive utilization of wheat bran according to claim 1, it is characterised in that：Step (3) described pH is 3.5, temperature For 50 DEG C, the pretreatment enzyme is the one or more in Viscozyme, complex cellulase and endoglucanase, described The dosage for pre-processing enzyme is 0.5 ‰ to 10 ‰ of wheat bran material quality in step (2).
- 5. the method for comprehensive utilization of wheat bran according to claim 4, it is characterised in that：The use of step (3) the pretreatment enzyme Measure as 1 ‰ to 5 ‰ of wheat bran material quality in step (2).
- 6. the method for comprehensive utilization of wheat bran according to claim 1, it is characterised in that：Step (4) described pH is 6.0.
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| CN115606813A (en) * | 2022-10-13 | 2023-01-17 | 中国林业科学研究院林产化学工业研究所 | Myrtle dietary fiber with anti-saccharification activity and preparation method thereof |
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