CN104651416A - Method for synchronously producing biological butanol and nano cellulose from sweet sorghum - Google Patents
Method for synchronously producing biological butanol and nano cellulose from sweet sorghum Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
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Abstract
The invention relates to a method for synchronously producing biological butanol and nano cellulose from sweet sorghum. The method comprises the following steps: juicing sweet sorghum straw to obtain sweet sorghum straw slag and a sweet sorghum juice; carrying out steam explosion on the sweet sorghum straw slag to obtain steam explosion straw and a steam explosion straw solution; carrying out enzymolysis on the steam explosion straw with a composite enzyme, washing with water, centrifuging, adding a small amount of water to prepare a nano cellulose suspension, preparing nano cellulose under high-pressure homogenization conditions, and finally, carrying out acetate modification on the nano cellulose surface; and mixing the steam explosion straw solution, water washing solution and sweet sorghum juice, controlling the sugar content, adding an inorganic salt, an N source and the like to prepare a butanol fermentation culture medium, inoculating a butanol fermentation seed solution, and carrying out butanol fermentation. The method provided by the invention is continuous and efficient, is environment-friendly and clean in the production process due to the adoption of the biological method, and has favorable comprehensive benefits as compared with the butanol produced from cellulose.
Description
Technical field
Present method belongs to straw resource utilization field, particularly a kind of method utilizing sweet sorghum synchronous production biological butanol and nano-cellulose.
Background technology
The following problem of tradition butylic fermentation industry ubiquity: (1) butanols output, productive rate are low.(2) solvent final concentration is low.This is the key point of butanols high cost.(3) ratio of butanols in total solvent is low, and the cost of later stage Butanol Recycling, separation is higher.(4) traditional butylic fermentation generally adopts corn, molasses to be that grain raw material is produced, and along with the scarcity of world food and the rise of provision price, the cost of butylic fermentation improves.Early stage butanols industry because of its fermentation costs high, be defeated by and decline in petrochemicals, this is also the bottleneck place limiting its extensive development now.
Nano-cellulose is a kind of novel polymer-function material, and its diameter is between 1nm to 100 nm, and length is 100 nm-500nm.Nano-cellulose has unique structure and excellent performance, biology, medical science, toughener, paper industry, purification, conduction, with inorganics compound, foodstuffs industry, magnetic composite etc. in extensive application, be described as " the magical material " that redeem the world.
Summary of the invention
The object of the present invention is to provide a kind of method utilizing sweet sorghum synchronous production biological butanol and nano-cellulose, reduce the cost with sweet sorghum production biological butanol, have good social benefit and overall economic efficiency, concrete technical scheme is as follows:
A) adopt juice extractor to squeeze the juice process to sweet sorghum stalk, obtain sweet sorghum juice and stalk slag;
B) stalk slag step a) obtained, steam explosion process 2 ~ 25 min under 1 ~ 5 MPa pressure condition, controls to dodge quick-fried process time and is less than 0.1 second, obtain steam puffed stalk and steam puffed stalk liquid;
C) steam puffed stalk washing step b) obtained, washing amount is heavy 1 ~ 2 times of steam puffed stalk, and water lotion is through 20-40 order Plate Filtration, collect water lotion, the sweet sorghum juice obtained with step a), the steam puffed stalk liquid that step b) obtains merges, and obtains mixed straw liquid;
D) add water in the steam puffed stalk obtained to step c), amount of water is 1 ~ 2 times of steam puffed stalk weight, add cellulase again, the complex enzyme zymohydrolysis of laccase and zytase, zymolyte through 20 ~ 40 order Plate Filtrations, washing (amount of water is heavy 1 ~ 2 times of steam puffed stalk), again after 20 ~ 40 order Plate Filtrations, the enzymolysis stalk fibre obtained, and the mixed straw liquid of water lotion and step c) is merged, obtain butylic fermentation raw sugar liquid.
E) add water (weight of water is 2 ~ 4 times of stalk fibre weight) in enzymolysis stalk fibre step d) obtained, and makes nano-cellulose suspension, through high-pressure homogeneous, make nano-cellulose; Then limit is stirred, while add Virahol, acetic anhydride and sulfuric acid mixture liquid in the nano-cellulose aqueous solution, three's weight is respectively heavy 3 ~ 5 times, 4 ~ 6 times and 4 ~ 6% of stalk fibre, temperature of reaction 30 ~ 60 DEG C, time 1 ~ 3 h, centrifugal, ultrasonic, adjust ph, to neutral, obtains preparing acetic acid esterified modified nano-cellulose.
F) step d) is obtained butylic fermentation raw sugar liquid tap water and adjust sugar, make the content of sugar 4 ~ 8%, then add calcium carbonate 5 ~ 9 g/L, dipotassium hydrogen phosphate 1.0 ~ 1.5 g/L, potassium primary phosphate 1.3 ~ 1.7 g/L, magnesium sulfate 0.2 ~ 0.3 g/L, ferric sulfate 0.008 ~ 0.012 g/L, paraxin 0.1 ~ 0.12 g/L, N sources etc., are adjusted to pH 6.5 ~ 7 with NaOH, sterilizing 20 min at 121 DEG C, make butanol fermentation culture medium, after inoculation butylic fermentation seed liquor, carry out butylic fermentation.
Further step a) described in method, it is characterized in that the described equipment of squeezing the juice is screw juice extractor, processing power 2-3 ton/h, power 10-15 kw.
Method described in step a), is characterized in that described raw material is sweet sorghum.
Further, compound enzymic preparation adding proportion 0.05 ~ 0.15%(described in step d) by weight), enzyme ratio (by weight) cellulase: laccase: zytase is (1.2 ~ 1.5): (1.1 ~ 1.2): (0.8 ~ 1.0), cellulose enzyme activity is 200,000 u/g, laccase activity 50,000 u/g, zytase 50,000 u/g; Enzyme digestion reaction temperature 40 ~ 75 DEG C, reaction times 5 ~ 20 h.
Further, the method described in step e), the pressure described in its feature during high-pressure homogeneous process is 50 ~ 150Mpa, circulates 4 ~ 8 times.
Further, the nitrogenous source in step (f) is any one in corn steep liquor, rapeseed cake, wheat bran, dregs of beans, gluten powder, rice bran, yeast powder, ammonium chloride Secondary ammonium phosphate, ammonium sulfate or composition.The adding proportion of nitrogenous source is: the 10:1 ~ 20:1 ratio of protein content (the sugared content with).
Further, method described in step f), it is characterized in that described butylic fermentation bacterial classification used is clostridium acetobutylicum (Clostridium acetobutylicum) CICC 8012, the one of clostridium saccharobutyricum (Clostridium saccharoacetobutylicum) or mixing.The compound method of butylic fermentation seed liquor: access in the corn culture medium of 7% by clostridium acetobutylicum (Clostridium acetobutylicum) CICC 8012 or clostridium saccharobutyricum (Clostridium saccharoacetobutylicum), 38 DEG C of anaerobically fermenting 18 h obtain seed liquor.
Further, the method described in step f), described butylic fermentation temperature is 35 ~ 39 DEG C, and the inoculum size of bacterial strain is 5 ~ 8%, fermentation time 48 ~ 80 h.
Sweet sorghum, also known as " two generation sugarcane ", containing abundant sugar juice, per mu yield sugarcane 20,000 kilograms, because its output is large, price cheapness is the optimum feed stock developing bioenergy.In " Renewable Energy Development Eleventh Five-Year Plan ", sweet sorghum is listed in first source of biological liquid fuel.By fully utilizing bar slag etc. discarded after fermentation, improve the comprehensive value of sweet sorghum, also reducing the cost of production of butanol accordingly, thus realizing energy development in pluralism, meeting the demand of domestic and international market to biofuel.
The present invention is a kind of method utilizing sweet sorghum synchronous production biological butanol and nano-cellulose, (1) initial gross separation of stalk liquid and stalk slag is first realized by squeezing, by the quick-fried Treating straw slag of vapour, object enters stalk fibre inside by high pressure molecule, with fried loose form by material explosion, achieving stalk liquid is separated simultaneously with the further of stalk fibre, also while destroying the imporosity of the stalk fibre that dissociates, increase the contact area of stalk fibre, be conducive to follow-up enzymolysis, the main purpose of enzymolysis removes the xylogen in stalk fibre, xylan and indefinite form Mierocrystalline cellulose, purifying crystalline cellulose, by high-pressure homogeneous, cellulosic structure is made to reach the requirement of nano-cellulose, pass through acetic acid esterified again, obtain the modified nanometer cellulose with good dispersion and glossiness.(2) by water lotion, enzymolysis solution and the mixing of stalk liquid glucose, the content controlling sugar, at 4%-8%, has been saved with water, has been improve the utilization to sugar in stalk, and then improve the comprehensive utilization value of stalk, have good economic benefit and social benefit.
Accompanying drawing explanation
Fig. 1 is process flow sheet of the present invention.
Embodiment
Clostridium acetobutylicum (Clostridium acetobutylicum) CICC 8012 used in the present invention and clostridium saccharobutyricum (Clostridium saccharoacetobutylicum) are all purchased from China General Microbiological culture presevation administrative center.
Embodiment 1
Get 1000 kg expressed juice of sweet sorghum stalks, obtain sweet sorghum juice and stalk slag, stalk slag is processed 8 min under pressure is 2.0 MPa conditions, obtains steam puffed stalk liquid and steam puffed stalk; The washing of steam puffed stalk 2000kg, water temperature 60 DEG C, washing time 2 h.Steam puffed stalk liquid is through 20 order Plate Filtrations, and filtrate and sweet sorghum juice, elutant merge, and obtain mixed straw liquid, filter residue is incorporated to steam puffed stalk.Add water in steam puffed stalk, amount of water is 1.5 times of steam puffed stalk weight, add compound enzymic preparation enzymolysis again, compound enzymic preparation adding proportion 0.08%(by weight), enzyme ratio (by weight) cellulase: laccase: zytase is 1.2:1.1:0.8, temperature of reaction 45 DEG C, reaction times 10 h.Zymolyte is through 40 order Plate Filtrations, and washing (amount of water is heavy 1 times of steam puffed stalk, water temperature 60 DEG C), then after 40 order Plate Filtrations, obtain enzymolysis Mierocrystalline cellulose, and being merged by the mixed straw liquid that water lotion and upper step obtain, obtains butylic fermentation raw sugar liquid.In enzymolysis Mierocrystalline cellulose, add the water of 2 times of weight, make cellulose suspension, under 100 MPa pressure conditions, circulate 6 times, namely obtain nano-cellulose suspension.Then limit is stirred, while add Virahol, acetic anhydride and sulfuric acid mixture liquid in the nano-cellulose aqueous solution, three's weight is respectively heavy 3 times, 4 times and 4% of stalk fibre, temperature of reaction 45 DEG C, time 3 h, centrifugal, ultrasonic, adjust ph, to neutral, obtains preparing acetic acid esterified modified nano-cellulose.Gained nano-cellulose fiber esterification substitution value is 0.03, and its diameter is between 35-40 nm, and length is 140-165 nm, has good dispersiveness and glossiness.
The butylic fermentation raw sugar liquid that upper step is obtained, adjusting sugared content with tap water is 6%, then adds calcium carbonate 6 g/L, dipotassium hydrogen phosphate 1.3 g/L, potassium primary phosphate 1.5 g/L, magnesium sulfate 0.25 g/L, ferric sulfate 0.01 g/L, paraxin 0.12 g/L, corn steep liquors etc., make sugared content be 18:1 with the ratio of protein content, then use NaOH adjust pH to 6.5, sterilizing 20 min at 121 DEG C, obtained fermention medium.Clostridium saccharobutyricum (Clostridium saccharoacetobutylicum) is accessed in the corn culture medium of 7%, anaerobically fermenting 14 h obtains seed liquor, seed liquor accesses in fermention medium by the inoculum size according to 7.5%, anaerobically fermenting 80 h at 39 DEG C, stops fermentation, with the solvent in gas chromatographic detection fermented liquid, total solvent reaches 21 g/L, wherein butanol content 13.3g/L, acetone 5.83 g/L, ethanol 1.87 g/L.。
The described equipment of squeezing the juice is screw juice extractor, processing power 2-3 ton/h, power 10-15 kw.
The preparation method of 7% described corn culture medium: measure 1000 mL water, be heated to 70 DEG C, adds 70 g Semen Maydis powder, maintain the temperature at about 60 DEG C 1 hour, after filtered through gauze, add appropriate water and supply 1000 mL, 121 DEG C of sterilizing 20 min, i.e. obtained corn culture medium.
Embodiment 2:
Get 1000 kg expressed juice of sweet sorghum stalks, obtain sweet sorghum juice and stalk slag, stalk slag is processed 6 min under pressure is 4.0 MPa conditions, obtains steam puffed stalk liquid and steam puffed stalk; The steam puffed stalk washing of 1600 kg, water temperature 58 DEG C, washing time 2 h.Steam puffed stalk liquid is through 30 order Plate Filtrations, and filtrate and sweet sorghum juice, elutant merge, and obtain mixed straw liquid, filter residue is incorporated to steam puffed stalk.Add water in steam puffed stalk, amount of water is 1.2 times of steam puffed stalk weight, add compound enzymic preparation enzymolysis again, compound enzymic preparation adding proportion 0.1%(by weight), enzyme ratio (by weight) cellulase: laccase: zytase is 1.1:1.0:0.9, temperature of reaction 45 DEG C, reaction times 12 h.Zymolyte is through 40 order Plate Filtrations, and washing (amount of water is heavy 1.2 times of steam puffed stalk, water temperature 60 DEG C), then after 40 order Plate Filtrations, obtain enzymolysis Mierocrystalline cellulose, and being merged by the mixed straw liquid that water lotion and upper step obtain, obtains butylic fermentation raw sugar liquid.In enzymolysis Mierocrystalline cellulose, add the water of 2.5 times of weight, make cellulose suspension, under 120 MPa pressure conditions, circulate 4 times, namely obtain nano-cellulose suspension.Then limit is stirred, while add Virahol, acetic anhydride and sulfuric acid mixture liquid in the nano-cellulose aqueous solution, three's weight is respectively heavy 4 times, 4.5 times and 5% of stalk fibre, temperature of reaction 40 DEG C, time 2.5 h, centrifugal, ultrasonic, adjust ph, to neutral, obtains preparing acetic acid esterified modified nano-cellulose.Gained nano-cellulose fiber esterification substitution value is 0.045, and its diameter is between 25-35 nm, and length is 130-140 nm, has good dispersiveness and glossiness.
The butylic fermentation raw sugar liquid that upper step is obtained, adjusting sugared content with tap water is 7%, then adds calcium carbonate 7 g/L, dipotassium hydrogen phosphate 1.5 g/L, potassium primary phosphate 1.3 g/L, magnesium sulfate 0.23 g/L, ferric sulfate 0.01 g/L, paraxin 0.11 g/L, corn steep liquors etc., make sugared content be 19:1 with the ratio of protein content, then use NaOH adjust pH to 7.0, sterilizing 20 min at 121 DEG C, obtained fermention medium.Clostridium saccharobutyricum (Clostridium saccharoacetobutylicum) is accessed in the corn culture medium of 7%, anaerobically fermenting 12 h obtains seed liquor, according to the inoculum size of 8%, seed liquor is accessed fermention medium, anaerobically fermenting 72 h at 39 DEG C, stops fermentation, with the solvent in gas chromatographic detection fermented liquid, total solvent reaches 20.5 g/L, wherein butanol content 13.0g/L, acetone 5.7 g/L, ethanol 1.8 g/L.
The described equipment of squeezing the juice is screw juice extractor, processing power 2-3 ton/h, power 10-15 kw.
The preparation method of 7% described corn culture medium: measure 1000 mL water, be heated to 70 DEG C, adds 70 g Semen Maydis powder, maintain the temperature at about 60 DEG C 1 hour, after filtered through gauze, add appropriate water and supply 1000 mL, 121 DEG C of sterilizing 20 min, i.e. obtained corn culture medium.
Embodiment 3:
Get 1000 kg expressed juice of sweet sorghum stalks, obtain sweet sorghum juice and stalk slag, stalk slag is processed 8 min under pressure is 2.0 MPa conditions, obtains steam puffed stalk liquid and steam puffed stalk; The steam puffed stalk washing of 1400 kg, water temperature 60 DEG C, washing time 2 h.Steam puffed stalk liquid is through 20 order Plate Filtrations, and filtrate and sweet sorghum juice, elutant merge, and obtain mixed straw liquid, filter residue is incorporated to steam puffed stalk.Add water in steam puffed stalk, amount of water is 1.5 times of steam puffed stalk weight, add compound enzymic preparation enzymolysis again, compound enzymic preparation adding proportion 0.08%(by weight), enzyme ratio (by weight) cellulase: laccase: zytase is 1.2:1.1:0.8, temperature of reaction 45 DEG C, reaction times 10 h.Zymolyte is through 40 order Plate Filtrations, and washing (amount of water is heavy 1.4 times of steam puffed stalk, water temperature 60 DEG C), then after 40 order Plate Filtrations, obtain enzymolysis Mierocrystalline cellulose, and being merged by the mixed straw liquid that water lotion and upper step obtain, obtains butylic fermentation raw sugar liquid.In enzymolysis Mierocrystalline cellulose, add the water of 3 times of weight, make cellulose suspension, under 150 MPa pressure conditions, circulate 3 times, namely obtain nano-cellulose suspension.Then limit is stirred, while add Virahol, acetic anhydride and sulfuric acid mixture liquid in the nano-cellulose aqueous solution, three's weight is respectively heavy 4 times, 4.5 times and 5% of stalk fibre, temperature of reaction 40 DEG C, time 2.5 h, centrifugal, ultrasonic, adjust ph, to neutral, obtains preparing acetic acid esterified modified nano-cellulose.Gained nano-cellulose fiber esterification substitution value is 0.05, and its diameter is between 20-30 nm, and length is 115-130 nm, has good dispersiveness and glossiness.
The butylic fermentation raw sugar liquid that upper step is obtained, adjusting sugared content with tap water is 7%, adds calcium carbonate 8 g/L, dipotassium hydrogen phosphate 1.1 g/L, potassium primary phosphate 1.6g/L, magnesium sulfate 0.25 g/L, ferric sulfate 0.011 g/L, paraxin 0.11 g/L, corn steep liquors etc., make sugared content be 18:1 with the ratio of protein content, then use NaOH adjust pH to 6.5, sterilizing 20 min at 121 DEG C, obtained fermention medium.Just clostridium acetobutylicum (Clostridium acetobutylicum) CICC 8012 accesses in the corn culture medium of 7%, anaerobically fermenting 13 h obtains seed liquor, according to the inoculum size of 8%, seed liquor is accessed fermention medium, anaerobically fermenting 74 h at 38 DEG C, stops fermentation, with the solvent in gas chromatographic detection fermented liquid, total solvent reaches 22 g/L, wherein butanol content 13.3 g/L, acetone 6.6 g/L, ethanol 2.1 g/L.
The described equipment of squeezing the juice is screw juice extractor, processing power 2-3 ton/h, power 10-15 kw.
The preparation method of 7% described corn culture medium: measure 1000 mL water, be heated to 70 DEG C, adds 70 g Semen Maydis powder, maintain the temperature at about 60 DEG C 1 hour, after filtered through gauze, add appropriate water and supply 1000 mL, 121 DEG C of sterilizing 20 min, i.e. obtained corn culture medium.
In sum, be only preferred embodiment of the present invention, not be used for limiting scope of the invention process, all equalizations of doing according to shape, structure, feature and the spirit described in the claims in the present invention scope change and modify, and all should be included in right of the present invention.
Claims (9)
1. utilize a method for sweet sorghum synchronous production biological butanol and nano-cellulose, it is characterized in that, comprise the steps:
A, process is rolled to sweet sorghum stalk, obtains sweet sorghum juice and stalk slag;
B, described stalk slag is carried out steam explosion process under the pressure of 1 ~ 5MPa, control to dodge quick-fried process time and be less than 0.1s, obtain steam puffed stalk and steam puffed stalk liquid;
C, described steam puffed stalk to be washed, to collect after water lotion after filtering, merge with described sweet sorghum juice, steam puffed stalk liquid, obtain mixed straw liquid;
Add water in d, steam puffed stalk after washing, add the mixed enzyme be made up of cellulase, laccase and zytase again, enzymolysis is carried out at 40 ~ 75 DEG C, zymolyte is first washed after filtering, filter again, obtain enzymolysis stalk fibre, water lotion and described mixed straw liquid are merged simultaneously, obtain butylic fermentation raw sugar liquid;
E, add water in described enzymolysis stalk fibre, make enzymolysis stalk fibre suspension, homogeneous under the pressure of 50 ~ 150MPa, makes the nano-cellulose aqueous solution; Then limit is stirred, while add the mixed solution be made up of Virahol, acetic anhydride and sulfuric acid in the described nano-cellulose aqueous solution, carry out centrifugal, ultrasonic at 30 ~ 60 DEG C after reaction times 1 ~ 3 h, adjust ph, to neutral, obtains preparing acetic acid esterified modified nano-cellulose;
F, described butylic fermentation raw sugar liquid tap water is adjusted sugar, make the content of sugar at 4 ~ 8wt%, add calcium carbonate, dipotassium hydrogen phosphate, potassium primary phosphate, magnesium sulfate, ferric sulfate, paraxin, N source again, being adjusted to pH is 6.5 ~ 7, sterilizing 20 min at 121 DEG C, make butanol fermentation culture medium, after inoculation butylic fermentation seed liquor, carry out butylic fermentation.
2. preparation method as claimed in claim 1, it is characterized in that, the water yield added when washing described in step c is 1 ~ 2 times of steam puffed stalk weight.
3. preparation method as claimed in claim 1, it is characterized in that, in step e, the consumption of described Virahol, acetic anhydride and sulfuric acid is respectively 3 ~ 5 times, 4 ~ 6 times and 4 ~ 6% of enzymolysis stalk fibre weight.
4. preparation method as claimed in claim 1, it is characterized in that, in step f, the dosage of described calcium carbonate, dipotassium hydrogen phosphate, potassium primary phosphate, magnesium sulfate, ferric sulfate, paraxin is respectively often liter of solution 5 ~ 9g, 1.0 ~ 1.5g, 1.3 ~ 1.7g, 0.2 ~ 0.3g, 0.008 ~ 0.012g, 0.10 ~ 0.12g.
5. preparation method as claimed in claim 1, it is characterized in that, the dosage of the mixed enzyme described in steps d is 0.05 ~ 0.15% of the weight of the mixture of steam puffed stalk and water.
6. the preparation method as described in claim 1 or 5, it is characterized in that, in described mixed enzyme, the mass ratio of cellulase, laccase and zytase is (1.2 ~ 1.5): (1.1 ~ 1.2): (0.8 ~ 1.0), wherein, the enzyme work of described cellulase is 200,000 u/g, laccase activity 50,000 u/g, zytase 50,000 u/g.
7. preparation method as claimed in claim 1, it is characterized in that, the nitrogenous source described in step f is any one or multiple in corn steep liquor, rapeseed cake, wheat bran, dregs of beans, gluten powder, rice bran, yeast powder, ammonium chloride, Secondary ammonium phosphate, ammonium sulfate.
8. preparation method as claimed in claim 1, it is characterized in that, butylic fermentation described in step f bacterial classification used is clostridium acetobutylicum (Clostridium acetobutylicum) CICC 8012, the one of clostridium saccharobutyricum (Clostridium saccharoacetobutylicum) or mixing; The compound method of described butylic fermentation seed liquor: access in the corn culture medium of 7wt% by clostridium acetobutylicum (Clostridium acetobutylicum) CICC 8012 or clostridium saccharobutyricum (Clostridium saccharoacetobutylicum), at 38 DEG C, anaerobically fermenting 18 h obtains seed liquor.
9. preparation method as claimed in claim 1, it is characterized in that, the butylic fermentation temperature in step f is 35 ~ 39 DEG C, and the inoculum size of bacterial strain is 5 ~ 8%, fermentation time 48 ~ 80 h.
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| CN109024031A (en) * | 2018-10-12 | 2018-12-18 | 山东金胜粮油集团有限公司 | The method that steam blasting combination ultrasonic treatment prepares peanut shell nano-cellulose |
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| CN106047948A (en) * | 2016-07-12 | 2016-10-26 | 广西南宁荣威德新能源科技有限公司 | Method for utilizing rosa banksiae to co-produce ethyl alcohol, acetone and butanol |
| CN108690853A (en) * | 2017-04-05 | 2018-10-23 | 中国石油化工股份有限公司 | A kind of method of fermenting and producing butanol |
| CN108690853B (en) * | 2017-04-05 | 2021-10-08 | 中国石油化工股份有限公司 | Method for producing butanol by fermentation |
| CN109024031A (en) * | 2018-10-12 | 2018-12-18 | 山东金胜粮油集团有限公司 | The method that steam blasting combination ultrasonic treatment prepares peanut shell nano-cellulose |
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