CN106047948A - Method for utilizing rosa banksiae to co-produce ethyl alcohol, acetone and butanol - Google Patents
Method for utilizing rosa banksiae to co-produce ethyl alcohol, acetone and butanol Download PDFInfo
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- CN106047948A CN106047948A CN201610544795.1A CN201610544795A CN106047948A CN 106047948 A CN106047948 A CN 106047948A CN 201610544795 A CN201610544795 A CN 201610544795A CN 106047948 A CN106047948 A CN 106047948A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
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- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
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Abstract
The invention discloses a method for utilizing rosa banksiae to co-produce ethyl alcohol, acetone and butanol and belongs to the technical field of biomass energy. The ethyl alcohol, the acetone and the butanol are prepared through the steps of rosa banksiae drying, smashing, pretreatment, hydrolysis, fermentation and the like. The raw material is wide in source, and the cost for co-producing the ethyl alcohol, the acetone and the butanol is low. The glucose conversion rate of the butanol prepared by means of the method is 92.22%-92.93%, the yield of the butanol is 66.54%-67.57%, the purity of the butanol is 99.59%-99.71%, it is shown that the glucose conversion rate and the yield of the butanol are high, and the high-yield ethyl alcohol, acetone and butanol can be achieved by means of the method. By the adoption of the method, a variety of new energy resources can be co-produced, and a new solution is provided for the energy shortage problem.
Description
[technical field]
The invention belongs to biomass energy technology field, be specifically related to a kind of Radix Aucklandiae rattan co-producing ethanol, acetone and butanol of utilizing
Method.
[background technology]
Along with the growth of population, grain, energy and environment are the main challenges of facing mankind.Producing with cellulosic material can be again
Raw clean energy resource is increasingly subject to extensive concern, and with agricultural waste material for raw material production Fuel butanol, ethanol etc. is considered as
The most promising industry.In Radix Aucklandiae rattan, protein content is low but carbohydrate content high, individual plants carbohydrate
Content is up to the 78.89% of dry, thus is the quality raw materials producing biomass energy.Both at home and abroad climing rattan is converted Fuel butanol,
The research of ethanol starts to walk, and current butanol, alcohol yied also ratio is relatively low, and bottleneck is that Radix Aucklandiae rattan is degraded into the technology of fermentable sugars
The most undesirable.Therefore, the comprehensive exploitation to Radix Aucklandiae rattan of the efficient degradation technique of invention Radix Aucklandiae rattan and the energy of lignocellulose
Utilize and be respectively provided with high economic worth and social benefit.
Utilize lignocellulosic material to produce biological butanol has important meaning (biological to alleviating energy crisis and ecological deterioration
Matter Chemical Engineering, 2012,46 (3), 39-44).But during enzyme hydrolysis, due to portion of cellulose enzyme and hemicellulase not
Reversible adsorbs the loss that can cause enzymatic activity on substrate particularly lignin surface, cellulose in addition in the way of ineffective adsorption
The crystalline texture of itself and the complexity of hemicellulose structure are also the key factors causing hydrolysis efficiency low.
In actual applications, bio-fuel butanol can mix, again without carrying out vehicle with any ratio by gasoline due to it
Transformation, and its economy is high, can be effectively improved fuel efficiency and the distance travelled of vehicle.Therefore bio-fuel butanol compares bio-ethanol
The most superior quality and higher calorific value conversion ratio.Thus, bio-fuel butanol will more win the favor of people.Pass
The preparation method of the biological butanol of system is prepared from by fermentation with grains such as Semen Maydis, wheat and barley, Semen sojae atricolor for raw material.But
Not only can not satisfy social needs with grain for raw material production bio-fuel, and entail dangers to grain security.Research worker is had to refer to
Go out, even if all Semen Maydiss of U.S.'s plantation and Semen sojae atricolor are all used for producing bioenergy, also can only meet American society's gasoline respectively
The 12% and the 6% of diesel oil demand of demand.And Semen Maydis and Semen sojae atricolor first have to meet grain, feedstuff and other economic needs, can not
Can all be used for producing bio-fuel.International Monetary Fund also warns, the whole world produces constantly for the corn of bio-fuel
Increase likely world's poverty to be produced and have a strong impact on.
[summary of the invention]
The technical problem to be solved in the present invention is to provide and a kind of utilizes Radix Aucklandiae rattan co-producing ethanol, acetone and the method for butanol, to solve
Radix Aucklandiae rattan of determining is degraded into fermentable sugars and not exclusively causes the problems such as ethanol, acetone and butanol productivity are low.Raw material sources of the present invention
Extensively, co-producing ethanol, acetone and butanol low cost；The method multiple new forms of energy of energy coproduction of the present invention, provide for energy scarcity problem
A kind of new solution.
In order to solve above technical problem, the present invention by the following technical solutions:
A kind of utilize Radix Aucklandiae rattan co-producing ethanol, acetone and the method for butanol, comprise the following steps:
S1: use saline solution rinsing Radix Aucklandiae rattan 1.5-2h, after rinsing, Radix Aucklandiae rattan is placed at temperature is 92-102 DEG C and dries to containing
The water yield≤8%, prepares dry Radix Aucklandiae rattan；
S2: dry Radix Aucklandiae rattan step S1 prepared is pulverized, and sifts out, and prepares Radix Aucklandiae rattan granule；
S3: put in microwave reactor by the Radix Aucklandiae rattan granule that step S2 prepares, adds solution A mix homogeneously, is heated to 56-66
DEG C, pretreatment 0.6-1.8h under speed of agitator is 100-400r/min, filters after being then cooled to room temperature, prepares pretreatment
Radix Aucklandiae rattan granule；Described solution A is the mixed liquor of butanone, ethanol, ammonium carboxylate salt, and the mass concentration of described butanone is 8%-10%,
The mass concentration of described ethanol is 60%-80%, and the mass concentration of described ammonium carboxylate salt is 6%-8%, and described butanone solution, ethanol are molten
Liquid, the volume ratio of ammonium carboxylate salts are 10-20:12-25:1-2, and described Radix Aucklandiae rattan granule is 1:6-with the solid-liquid ratio of solution A
9g/mL；
S4: the Radix Aucklandiae rattan granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mix homogeneously,
Regulation pH is 1.2-1.8, is heated to 68-75 DEG C, processes 1.2-2.5h, then cool down under speed of agitator is 100-500r/min
Filter to room temperature, prepare sugar liquid；Described solution B is the mixed liquor of carbon back sodium sulfonate, sulphuric acid, the quality of described carbon back sodium sulfonate
Concentration is 5%-7%, and the mass concentration of described sulphuric acid is 4%-6%, and described carbon back sodium sulfonate solution, the volume ratio of sulfuric acid solution are 6-
10:5-8, the Radix Aucklandiae rattan granule of described pretreatment and the solid-liquid ratio of solution B are 1:3-6g/mL；
S5: sugar liquid step S4 prepared accesses Clostridium beijerinckii ATCC55025, adds yeast powder, albumen, fat, propanoic acid ammonium, dimension
Raw element, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, regulation pH is 5.4-6.6, and controlling temperature is 28-32 DEG C of bottom fermentation 60-90h,
Prepare ethanol, acetone and butanol；Described Clostridium beijerinckii ATCC55025 mass is the 4%-8% of sugar liquid quality, described yeast powder, egg
In vain, fat, propanoic acid ammonium, vitamin, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride quality be respectively sugar liquid quality 0.03%-2%,
0.02%-0.5%、0.02%-0.5%、0.04%-0.6%、0.04%-0.5%、0.16%-0.24%、0.09%-0.14%、0.1%-
0.2%。
Further, the concentration of saline solution described in step S1 is 18%-25%.
Further, the mesh number of sieve described in step S2 is 120-180.
Further, the granule of Radix Aucklandiae rattan described in step S3 temperature of pretreatment in microwave reactor is 66 DEG C, is stirring
Mixing rotating speed is pretreatment 0.6h under 400r/min.
Further, the granule of Radix Aucklandiae rattan described in step S3 is 1:10g/mL with the solid-liquid ratio of solution A.
Further, the pH that the Radix Aucklandiae rattan granule of pretreatment described in step S4 processes at ultrasound reactor is 1.8, adds
Heat, to 75 DEG C, processes 1.2h under speed of agitator is 500r/min.
Further, the Radix Aucklandiae rattan granule of pretreatment described in step S4 and the solid-liquid ratio of solution B are 1:6g/mL.
Further, Clostridium beijerinckii ATCC55025 mass described in step S5 is the 6% of sugar liquid quality, described yeast powder,
Albumen, fat, propanoic acid ammonium, vitamin, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride quality be respectively the 1% of sugar liquid quality, 0.25%,
0.25%、0.3%、0.25%、0.2%、0.2%、0.15%。
The method have the advantages that
(1) raw material sources of the present invention is wide, co-producing ethanol, acetone and butanol low cost；
(2) preparation technology of the present invention is simple, less investment, is suitable for large-scale promotion and application；
(3) uncertainty of microbial enzyme fermentation sugar refining technology can be avoided, reduce technical risk；
(4) sugar conversion ratio is high, and the level of residue of generation is few, is environmental type technique；
(5) hydrolysis temperature is relatively low, and energy resource consumption is few, beneficially the promotion and application of correlation technique；
(6) the sugared yield that this method prepares is 92.22%-92.93%, and butanol yield is 66.54%-67.57%, butanol purity
For 99.59%-99.71%, illustrate that sugar conversion ratio and butanol yield are high, therefore, use the present invention method can high-yield ethanol, third
Ketone and butanol；
(7) the method multiple new forms of energy of energy coproduction of the present invention, provide a kind of new solution for energy scarcity problem.
[detailed description of the invention]
For ease of being more fully understood that the present invention, being illustrated by following example, these embodiments belong to the protection of the present invention
Scope, but it is not intended to protection scope of the present invention.
In an embodiment, described utilize Radix Aucklandiae rattan co-producing ethanol, acetone and the method for butanol, comprise the following steps:
S1: the saline solution rinsing Radix Aucklandiae rattan 1.5-2h using concentration to be 18%-25%, it is 92-that Radix Aucklandiae rattan is placed on after rinsing temperature
Dry to water content≤8% at 102 DEG C, prepare dry Radix Aucklandiae rattan；
S2: dry Radix Aucklandiae rattan step S1 prepared is pulverized, and crosses 120-180 mesh sieve, prepares Radix Aucklandiae rattan granule；
S3: put in microwave reactor by the Radix Aucklandiae rattan granule that step S2 prepares, adds solution A mix homogeneously, is heated to 56-66
DEG C, pretreatment 0.6-1.8h under speed of agitator is 100-400r/min, filters after being then cooled to room temperature, prepares pretreatment
Radix Aucklandiae rattan granule；Described solution A is the mixed liquor of butanone, ethanol, ammonium carboxylate salt, and the mass concentration of described butanone is 8%-10%,
The mass concentration of described ethanol is 60%-80%, and the mass concentration of described ammonium carboxylate salt is 6%-8%, and described butanone solution, ethanol are molten
Liquid, the volume ratio of ammonium carboxylate salts are 10-20:12-25:1-2, and described Radix Aucklandiae rattan granule is 1:6-with the solid-liquid ratio of solution A
9g/mL；
S4: the Radix Aucklandiae rattan granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mix homogeneously,
Regulation pH is 1.2-1.8, is heated to 68-75 DEG C, processes 1.2-2.5h, then cool down under speed of agitator is 100-500r/min
Filter to room temperature, prepare sugar liquid；Described solution B is the mixed liquor of carbon back sodium sulfonate, sulphuric acid, the quality of described carbon back sodium sulfonate
Concentration is 5%-7%, and the mass concentration of described sulphuric acid is 4%-6%, and described carbon back sodium sulfonate solution, the volume ratio of sulfuric acid solution are 6-
10:5-8, the Radix Aucklandiae rattan granule of described pretreatment and the solid-liquid ratio of solution B are 1:3-6g/mL；
S5: sugar liquid step S4 prepared accesses Clostridium beijerinckii ATCC55025, adds yeast powder, albumen, fat, propanoic acid ammonium, dimension
Raw element, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, regulation pH is 5.4-6.6, and controlling temperature is 28-32 DEG C of bottom fermentation 60-90h,
Prepare ethanol, acetone and butanol；Described Clostridium beijerinckii ATCC55025 mass is the 4%-8% of sugar liquid quality, described yeast powder, egg
In vain, fat, propanoic acid ammonium, vitamin, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride quality be respectively sugar liquid quality 0.03%-2%,
0.02%-0.5%、0.02%-0.5%、0.04%-0.6%、0.04%-0.5%、0.16%-0.24%、0.09%-0.14%、0.1%-
0.2%。
Below by more specifically embodiment, the present invention will be described.
Embodiment 1
A kind of utilize Radix Aucklandiae rattan co-producing ethanol, acetone and the method for butanol, comprise the following steps:
S1: the saline solution rinsing Radix Aucklandiae rattan 1.5-2h using concentration to be 22%, after rinsing, Radix Aucklandiae rattan is placed on temperature and is 92-102 DEG C
Lower drying, to water content≤8%, prepares dry Radix Aucklandiae rattan；
S2: dry Radix Aucklandiae rattan step S1 prepared is pulverized, and crosses 150 mesh sieve, prepares Radix Aucklandiae rattan granule；
S3: put in microwave reactor by the Radix Aucklandiae rattan granule that step S2 prepares, adds solution A mix homogeneously, is heated to 60 DEG C,
Pretreatment 1.2h under speed of agitator is 300r/min, filters after being then cooled to room temperature, prepares the Radix Aucklandiae rattan granule of pretreatment；
Described solution A is the mixed liquor of butanone, ethanol, ammonium carboxylate salt, and the mass concentration of described butanone is 9%, and the quality of described ethanol is dense
Degree is 70%, and the mass concentration of described ammonium carboxylate salt is 7%, described butanone solution, ethanol solution, the volume ratio of ammonium carboxylate salts
For 15:18:1, described Radix Aucklandiae rattan granule is 1:8g/mL with the solid-liquid ratio of solution A；
S4: the Radix Aucklandiae rattan granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mix homogeneously,
Regulation pH is 1.5, is heated to 72 DEG C, processes 1.8h under speed of agitator is 300r/min, filters, system after being then cooled to room temperature
Obtain sugar liquid；Described solution B is the mixed liquor of carbon back sodium sulfonate, sulphuric acid, and the mass concentration of described carbon back sodium sulfonate is 6%, described sulfur
The mass concentration of acid is 5%, and described carbon back sodium sulfonate solution, the volume ratio of sulfuric acid solution are 8:6, the Radix Aucklandiae rattan of described pretreatment
Granule is 1:2g/mL with the solid-liquid ratio of solution B；
S5: sugar liquid step S4 prepared accesses Clostridium beijerinckii ATCC55025, adds yeast powder, albumen, fat, propanoic acid ammonium, dimension
Raw element, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, regulation pH is 6, and controlling temperature is 30 DEG C of bottom fermentation 75h, prepares ethanol, acetone
And butanol；Described Clostridium beijerinckii ATCC55025 mass is the 6% of sugar liquid quality, described yeast powder, albumen, fat, propanoic acid ammonium, dimension
Raw element, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride quality be respectively the 1% of sugar liquid quality, 0.25%, 0.25%, 0.3%, 0.25%,
0.2%、0.2%、0.15%。
Embodiment 2
A kind of utilize Radix Aucklandiae rattan co-producing ethanol, acetone and the method for butanol, comprise the following steps:
S1: the saline solution rinsing Radix Aucklandiae rattan 2h using concentration to be 18%, after rinsing, Radix Aucklandiae rattan is placed at temperature is 92-102 DEG C baking
Do to water content≤8%, prepare dry Radix Aucklandiae rattan；
S2: dry Radix Aucklandiae rattan step S1 prepared is pulverized, and crosses 120 mesh sieve, prepares Radix Aucklandiae rattan granule；
S3: put in microwave reactor by the Radix Aucklandiae rattan granule that step S2 prepares, adds solution A mix homogeneously, is heated to 56 DEG C,
Pretreatment 1.8h under speed of agitator is 100r/min, filters after being then cooled to room temperature, prepares the Radix Aucklandiae rattan granule of pretreatment；
Described solution A is the mixed liquor of butanone, ethanol, ammonium carboxylate salt, and the mass concentration of described butanone is 8%, and the quality of described ethanol is dense
Degree is 60%, and the mass concentration of described ammonium carboxylate salt is 6%, described butanone solution, ethanol solution, the volume ratio of ammonium carboxylate salts
For 10:12:1, described Radix Aucklandiae rattan granule is 1:8g/mL with the solid-liquid ratio of solution A；
S4: the Radix Aucklandiae rattan granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mix homogeneously,
Regulation pH is 1.2, is heated to 68 DEG C, processes 2.5h under speed of agitator is 100r/min, filters, system after being then cooled to room temperature
Obtain sugar liquid；Described solution B is the mixed liquor of carbon back sodium sulfonate, sulphuric acid, and the mass concentration of described carbon back sodium sulfonate is 5%, described sulfur
The mass concentration of acid is 4%, and described carbon back sodium sulfonate solution, the volume ratio of sulfuric acid solution are 6:5, the Radix Aucklandiae rattan of described pretreatment
Granule is 1:3g/mL with the solid-liquid ratio of solution B；
S5: sugar liquid step S4 prepared accesses Clostridium beijerinckii ATCC55025, adds yeast powder, albumen, fat, propanoic acid ammonium, dimension
Raw element, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, regulation pH is 5.4, and controlling temperature is 28 DEG C of bottom fermentation 90h, prepared ethanol, third
Ketone and butanol；Described Clostridium beijerinckii ATCC55025 mass is the 4% of sugar liquid quality, described yeast powder, albumen, fat, propanoic acid ammonium,
Vitamin, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride quality be respectively the 0.03% of sugar liquid quality, 0.02%, 0.02%, 0.04%,
0.04%、0.16%、0.09%、0.1%。
Embodiment 3
A kind of utilize Radix Aucklandiae rattan co-producing ethanol, acetone and the method for butanol, comprise the following steps:
S1: the saline solution rinsing Radix Aucklandiae rattan 1.5h using concentration to be 25%, after rinsing, Radix Aucklandiae rattan is placed at temperature is 92-102 DEG C
Dry to water content≤8%, prepare dry Radix Aucklandiae rattan；
S2: dry Radix Aucklandiae rattan step S1 prepared is pulverized, and crosses 180 mesh sieve, prepares Radix Aucklandiae rattan granule；
S3: put in microwave reactor by the Radix Aucklandiae rattan granule that step S2 prepares, adds solution A mix homogeneously, is heated to 66 DEG C,
Pretreatment 0.6h under speed of agitator is 400r/min, filters after being then cooled to room temperature, prepares the Radix Aucklandiae rattan granule of pretreatment；
Described solution A is the mixed liquor of butanone, ethanol, ammonium carboxylate salt, and the mass concentration of described butanone is 10%, the quality of described ethanol
Concentration is 80%, and the mass concentration of described ammonium carboxylate salt is 8%, described butanone solution, ethanol solution, the volume of ammonium carboxylate salts
It is 1:9g/mL than the solid-liquid ratio for 20:25:2, described Radix Aucklandiae rattan granule and solution A；
S4: the Radix Aucklandiae rattan granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mix homogeneously,
Regulation pH is 1.8, is heated to 75 DEG C, processes 1.2h under speed of agitator is 500r/min, filters, system after being then cooled to room temperature
Obtain sugar liquid；Described solution B is the mixed liquor of carbon back sodium sulfonate, sulphuric acid, and the mass concentration of described carbon back sodium sulfonate is 7%, described sulfur
The mass concentration of acid is 6%, and described carbon back sodium sulfonate solution, the volume ratio of sulfuric acid solution are 10:8, the Radix Aucklandiae of described pretreatment
Rattan granule is 1:6g/mL with the solid-liquid ratio of solution B；
S5: sugar liquid step S4 prepared accesses Clostridium beijerinckii ATCC55025, adds yeast powder, albumen, fat, propanoic acid ammonium, dimension
Raw element, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, regulation pH is 6.6, and controlling temperature is 32 DEG C of bottom fermentation 60h, prepared ethanol, third
Ketone and butanol；Described Clostridium beijerinckii ATCC55025 mass is the 4%-8% of sugar liquid quality, described yeast powder, albumen, fat, propanoic acid
Ammonium, vitamin, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride quality be respectively the 2% of sugar liquid quality, 0.5%, 0.5%, 0.6%, 0.5%,
0.24%、0.14%、0.2%。
The sugar liquid taking out appropriate embodiment 1-3 is boiled, is inactivated, and appropriateness dilution, measures sugar fermentation by DNS colorimetric fixed sugar method
Content, and calculate sugar yield；The method of embodiment 1-3 is prepared gained ethanol, acetone and butanol mixed liquid purify simultaneously, calculate
Butanol yield and purity, result is as shown in the table:
| Embodiment | Sugar yield, % | Butanol yield, % | Butanol purity, % |
| 1 | 92.82 | 67 | 99.62 |
| 2 | 92.22 | 66.54 | 99.59 |
| 3 | 92.93 | 67.57 | 99.71 |
As seen from the above table, the method for embodiment of the present invention 1-3 the sugared yield prepared is 92.22%-92.93%, and butanol yield is
66.54%-67.57%, butanol purity is 99.59%-99.71%, illustrates that sugar conversion ratio and butanol yield are high, therefore, uses this
The method of invention can high-yield ethanol, acetone and butanol.
Above content it cannot be assumed that the present invention be embodied as be confined to these explanation, technology belonging to the present invention is led
For the those of ordinary skill in territory, without departing from the inventive concept of the premise, it is also possible to make some simple deduction or replace,
All should be considered as belonging to the scope of patent protection that the present invention is determined by the claims submitted to.
Claims (8)
1. one kind utilizes Radix Aucklandiae rattan co-producing ethanol, acetone and the method for butanol, it is characterised in that comprise the following steps:
S1: use saline solution rinsing Radix Aucklandiae rattan 1.5-2h, after rinsing, Radix Aucklandiae rattan is placed at temperature is 92-102 DEG C and dries to containing
The water yield≤8%, prepares dry Radix Aucklandiae rattan；
S2: dry Radix Aucklandiae rattan step S1 prepared is pulverized, and sifts out, and prepares Radix Aucklandiae rattan granule；
S3: put in microwave reactor by the Radix Aucklandiae rattan granule that step S2 prepares, adds solution A mix homogeneously, is heated to 56-66
DEG C, pretreatment 0.6-1.8h under speed of agitator is 100-400r/min, filters after being then cooled to room temperature, prepares pretreatment
Radix Aucklandiae rattan granule；Described solution A is the mixed liquor of butanone, ethanol, ammonium carboxylate salt, and the mass concentration of described butanone is 8%-10%,
The mass concentration of described ethanol is 60%-80%, and the mass concentration of described ammonium carboxylate salt is 6%-8%, and described butanone solution, ethanol are molten
Liquid, the volume ratio of ammonium carboxylate salts are 10-20:12-25:1-2, and described Radix Aucklandiae rattan granule is 1:6-with the solid-liquid ratio of solution A
9g/mL；
S4: the Radix Aucklandiae rattan granule of pretreatment step S3 prepared is put in ultrasound reactor, adds solution B mix homogeneously,
Regulation pH is 1.2-1.8, is heated to 68-75 DEG C, processes 1.2-2.5h, then cool down under speed of agitator is 100-500r/min
Filter to room temperature, prepare sugar liquid；Described solution B is the mixed liquor of carbon back sodium sulfonate, sulphuric acid, the quality of described carbon back sodium sulfonate
Concentration is 5%-7%, and the mass concentration of described sulphuric acid is 4%-6%, and described carbon back sodium sulfonate solution, the volume ratio of sulfuric acid solution are 6-
10:5-8, the Radix Aucklandiae rattan granule of described pretreatment and the solid-liquid ratio of solution B are 1:3-6g/mL；
S5: sugar liquid step S4 prepared accesses Clostridium beijerinckii ATCC55025, adds yeast powder, albumen, fat, propanoic acid ammonium, dimension
Raw element, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, regulation pH is 5.4-6.6, and controlling temperature is 28-32 DEG C of bottom fermentation 60-90h,
Prepare ethanol, acetone and butanol；Described Clostridium beijerinckii ATCC55025 mass is the 4%-8% of sugar liquid quality, described yeast powder, egg
In vain, fat, propanoic acid ammonium, vitamin, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride quality be respectively sugar liquid quality 0.03%-2%,
0.02%-0.5%、0.02%-0.5%、0.04%-0.6%、0.04%-0.5%、0.16%-0.24%、0.09%-0.14%、0.1%-
0.2%。
The most according to claim 1 utilize Radix Aucklandiae rattan co-producing ethanol, acetone and the method for butanol, it is characterised in that step
The concentration of saline solution described in S1 is 18%-25%.
The most according to claim 1 utilize Radix Aucklandiae rattan co-producing ethanol, acetone and the method for butanol, it is characterised in that step
The mesh number of sieve described in S2 is 120-180.
The most according to claim 1 utilize Radix Aucklandiae rattan co-producing ethanol, acetone and the method for butanol, it is characterised in that step
The granule of Radix Aucklandiae rattan described in S3 temperature of pretreatment in microwave reactor is 66 DEG C, locates in advance under speed of agitator is 400r/min
Reason 0.6h.
The most according to claim 1 utilize Radix Aucklandiae rattan co-producing ethanol, acetone and the method for butanol, it is characterised in that step
The granule of Radix Aucklandiae rattan described in S3 is 1:10g/mL with the solid-liquid ratio of solution A.
The most according to claim 1 utilize Radix Aucklandiae rattan co-producing ethanol, acetone and the method for butanol, it is characterised in that step
The pH that the Radix Aucklandiae rattan granule of pretreatment described in S4 processes at ultrasound reactor is 1.8, is heated to 75 DEG C, at speed of agitator is
1.2h is processed under 500r/min.
The most according to claim 1 utilize Radix Aucklandiae rattan co-producing ethanol, acetone and the method for butanol, it is characterised in that step
The Radix Aucklandiae rattan granule of pretreatment described in S4 and the solid-liquid ratio of solution B are 1:5g/mL.
The most according to claim 1 utilize Radix Aucklandiae rattan co-producing ethanol, acetone and the method for butanol, it is characterised in that step
Clostridium beijerinckii ATCC55025 mass described in S5 is the 6% of sugar liquid quality, and described yeast powder, albumen, fat, propanoic acid ammonium, dimension are raw
Element, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride quality be respectively the 1% of sugar liquid quality, 0.25%, 0.25%, 0.3%, 0.25%,
0.2%、0.2%、0.15%。
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Citations (3)
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|---|---|---|---|---|
| CN102352381A (en) * | 2011-10-08 | 2012-02-15 | 山东龙力生物科技股份有限公司 | Method using xylose production waste liquid to produce acetone and butanol |
| CN104651416A (en) * | 2015-02-09 | 2015-05-27 | 江苏联海生物科技有限公司 | Method for synchronously producing biological butanol and nano cellulose from sweet sorghum |
| CN104774877A (en) * | 2015-04-10 | 2015-07-15 | 山东龙力生物科技股份有限公司 | Method for co-producing ethanol, acetone and butanol by lignocellulose biomass |
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2016
- 2016-07-12 CN CN201610544795.1A patent/CN106047948A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352381A (en) * | 2011-10-08 | 2012-02-15 | 山东龙力生物科技股份有限公司 | Method using xylose production waste liquid to produce acetone and butanol |
| CN104651416A (en) * | 2015-02-09 | 2015-05-27 | 江苏联海生物科技有限公司 | Method for synchronously producing biological butanol and nano cellulose from sweet sorghum |
| CN104774877A (en) * | 2015-04-10 | 2015-07-15 | 山东龙力生物科技股份有限公司 | Method for co-producing ethanol, acetone and butanol by lignocellulose biomass |
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