CN103478410B - Probiotic protein feed produced through peltate yam rhizomes and production method thereof - Google Patents
Probiotic protein feed produced through peltate yam rhizomes and production method thereof Download PDFInfo
- Publication number
- CN103478410B CN103478410B CN201310422512.2A CN201310422512A CN103478410B CN 103478410 B CN103478410 B CN 103478410B CN 201310422512 A CN201310422512 A CN 201310422512A CN 103478410 B CN103478410 B CN 103478410B
- Authority
- CN
- China
- Prior art keywords
- starch
- yellow ginger
- cellulosic
- protein feed
- probio
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for producing probiotic protein feed through peltate yam rhizomes. The method comprises the following steps that (1) peltate yam rhizome cellulose and starch are separated; (2) the peltate yam rhizome cellulose is fermented; (3) the peltate yam rhizome starch is liquefied and saccharified; (4) the probiotic protein feed is prepared. Firstly, the starch and the cellulose in the peltate yam rhizomes are separated through a mechanical method, the cellulose is used for solid state fermentation to obtain fibrous residues containing cellulose and mycoprotein, then a suspension solution for separation of the starch is used for subsequent extraction of saponin, supernate is added to the peltate yam rhizome starch, and circulation utilization of waste water is achieved. Peltate yam rhizome starch milk is used for cultivating probiotics after being liquefied and saccharified, the probiotics include saccharomyces boulardii and lactobacillus acidophilus, then cellulose fermentation broth and probiotic culture are completely mixed to be uniform, and the probiotic protein feed containing the cellulose is obtained. Comprehensive utilization of the starch and the cellulose in the production process of the diosgenin saponin is completely achieved, production benefits are improved, and exhaust amount and pollution are reduced largely.
Description
Technical field
The present invention relates to a kind of probio protein feed and production method thereof, be specially a kind of probio protein feed and method thereof of utilizing yellow ginger to produce.
Background technology
Yellow ginger formal name used at school dioscorea zingiberensis wright (Dioscorea zingiberensis.C.H.Wright), has another name called duration and degree of heating root, is used as medicine with root-like stock, is China's tradition parts of generic medicinal plants, has the title of " medicinal gold ".In yellow ginger root-like stock, contain abundant Dioscin, its hydrolysate is that diosgenin is turmeric saponin, and the plant that saponin content is higher is in the world few, is mainly distributed in China and Mexico.At present, the saponin export volume of China is only second to Mexico, occupies the second in the world.
In yellow ginger except containing saponin(e, also containing the dioscorea zingiberensis pigment of the cellulose of the starch of 40%-50%, 40-50 and hemicellulose and lignin, 2% left and right, the organic matters such as 2% left and right plant essence, tannic acid.At present, yellow ginger is mainly used to produce saponin, but yellow ginger traditional processing technology causes environmental pollution, a lot of documents all Alternative improve, mainly follow-up acid treatment consumption will be reduced after starch and fiber separation, pollute thereby reduce, but Dioscorea. zingiberensis Wright Starch, cellulose etc. still could not find the suitable approach that utilizes.
Summary of the invention
The object of the present invention is to provide a kind of probio protein feed and method thereof of utilizing yellow ginger to produce, the accessory substance starch and the cellulose that utilize turmeric saponin to produce are prepared probio protein feed, have realized the resource of yellow ginger accessory substance, have improved economic benefit.
The present invention is achieved through the following technical solutions:
Utilize yellow ginger to produce a method for probio protein feed, comprise the steps,
1) yellow ginger cellulosic and starch separate: after yellow ginger is cleaned to pulverizing, filtration obtains cellulosic; To leach thing and leave standstill and be divided into three layers, upper strata is supernatant, and centre be suspension, and lower floor is starch, pours out supernatant for subsequent use, isolates lower floor's starch and also washs;
2) yellow ginger cellulosic fermentation: add wheat bran, ammonium sulfate and Tween 80 in yellow ginger cellulosic, and stir and obtain cellulosic mixture, then sterilizing is cooled to 35-40 DEG C of access Trichoderma viride, after 27-33 DEG C of constant temperature culture 3-4d, obtain the yellow ginger fibrous residue of cellulase and mycoprotein;
3) Dioscorea. zingiberensis Wright Starch liquefying-saccharifying: the Dioscorea. zingiberensis Wright Starch after washing is added to supernatant, making solid content in starch milk is 8-12%, its pH value is adjusted to 6.2-6.5, and is warming up to 70-80 DEG C, then add AMS and calcium chloride, and be incubated enzymolysis 1-2h, then cool the temperature to 55-60 DEG C, then pH value is adjusted to 5.4-6.0, and add carbohydrase and Pullulanase, insulation enzymolysis 3-4h, obtains Dioscorea. zingiberensis Wright Starch enzymolysis liquid;
4) probio protein feed preparation: Dioscorea. zingiberensis Wright Starch enzymolysis liquid is cooled to after 45-50 DEG C, adds (NH
4)
2sO
4, peptone, anhydrous sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, stir and regulate pH value to obtain mixed enzymolysis liquid to 5.0-6.0, access again Paula enlightening yeast and lactobacillus acidophilus, 34-39 DEG C of bottom fermentation 48-60h, after yellow ginger fibrous residue is added, mix, and low temperature drying obtains yellow ginger probio protein feed.
Further, in step 1), yellow ginger adopts the cadmium yellow ginger of water content 65-70%.
Further, in step 1), adopt 80-100 object filter-cloth filtering to obtain cellulosic, leach after thing leaves standstill 5-6h and be divided into three layers.
Further, step 2) in, in yellow ginger cellulosic, in every kilogram of yellow ginger cellulosic, add respectively in proportion the Tween 80 of the wheat bran of 20-25g and the ammonium sulfate of 10-20g and 0.8-1.2ml.
Further, step 2) in, sterilizing 20min-25min at the temperature of 121 DEG C, is cooled to, after 35 DEG C-40 DEG C, access in proportion the Trichoderma viride liquid spawn of 6-10ml in every 100mg cellulosic mixture.
Further, in step 3), add after AMS and calcium chloride, the vigor of corresponding every gram of starch AMS is 200-240U, the Ca in every kilogram of starch
2+ion concentration is 200-240mg.
Further, in step 3), add after carbohydrase and Pullulanase, the vigor of corresponding every gram of turmeric starch saccharification enzyme is 240-300U, and the vigor of Pullulanase is 20-40U.
Further, in step 4), add (the NH of 8-10g by every liter of Dioscorea. zingiberensis Wright Starch enzymolysis liquid
4)
2sO
4, the peptone of 4-5g, the anhydrous sodium acetate of 2-3.0g, the diammonium hydrogen citrate of 5-6g, the Tween 80 of 0.8-1.0mL, the magnesium sulfate of 0.2-0.3g, the manganese sulfate of 0.04-0.06g, the dipotassium hydrogen phosphate of 2.0-4.0g.
Further, in step 4), in every 100ml mixed enzymolysis liquid, access respectively in proportion Paula lactobacillus acidophilus bacterial classification enlightening yeast and 6-8ml of 4-6ml.
A probio protein feed that utilizes yellow ginger to produce, is made by the above method of utilizing yellow ginger to produce probio protein feed described in any one.
Compared with prior art, the present invention has following useful technique effect:
A kind of method of utilizing yellow ginger to produce probio protein feed of the present invention, first separates the starch in yellow ginger and cellulosic by mechanical means, cellulosic is obtained to the fibrous residue of cellulase and mycoprotein for solid state fermentation; Then the suspension during by separating starch is for the extraction of follow-up saponin, supernatant is joined in Dioscorea. zingiberensis Wright Starch, realize recycling of waste water, Dioscorea. zingiberensis Wright Starch breast through liquefaction, after saccharification for cultivating probio---Paula enlightening yeast and lactobacillus acidophilus, fully mix by cellulose fermentation thing and probiotic bacterial cultures the probio protein feed that obtains cellulase subsequently.
Simultaneously owing in the time that saponin is extracted production, starch and cellulosic being separated, therefore while production, acid hydrolysis volume and solid content greatly reduce, make sour use amount reduce 85-93%, hydrolysate is washed neutral water consumption and is also down to original 10-15%, the amount of gasoline that follow-up saponin is extracted is also down to original 10-20%, fully realize starch and cellulosic comprehensive utilization in turmeric saponin production process, improved productivity effect, greatly reduced discharge capacity and pollution.
Further, by the control to the vigor that adds enzyme in step and the control to other admixture quality, and the control of technological parameter, improve the content of active principle in protein feed, ensured the quality of product.
A kind of probio protein feed that utilizes yellow ginger to produce of the present invention, be rich in Paula enlightening yeast, lactobacillus acidophilus, Trichoderma viride and cellulase, not only can be the useful animal health of livestock probiotic supplemented, and cellulase in feed can also promote the absorption to nutriment and improve efficiency of feed utilization.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.
Example 1
Utilize yellow ginger to produce a method for probio protein feed, comprise the steps,
1) yellow ginger cellulosic and starch separate: after yellow ginger is cleaned to pulverizing, filtration obtains cellulosic; To leach thing and leave standstill and be divided into three layers, upper strata is supernatant, and centre be suspension, and lower floor is starch, pours out supernatant for subsequent use, isolates lower floor's starch and also washs; Preferably, yellow ginger adopts the cadmium yellow ginger of water content 65%; Concrete employing 100 object filter-cloth filterings obtain cellulosic, leach after thing leaves standstill 6h and are divided into three layers.
2) yellow ginger cellulosic fermentation: add wheat bran, ammonium sulfate and Tween 80 in yellow ginger cellulosic, and stir and obtain cellulosic mixture, then sterilizing is cooled to 35 DEG C of access Trichoderma virides, after 30 DEG C of constant temperature culture 3d, obtains the yellow ginger fibrous residue of cellulase and mycoprotein; Concrete, in yellow ginger cellulosic, in every kilogram of yellow ginger cellulosic, add respectively in proportion the Tween 80 of the wheat bran of 20g and the ammonium sulfate of 10g and 0.8ml; Sterilizing 20min at the temperature of 121 DEG C, is cooled to after 35 DEG C, accesses in proportion the Trichoderma viride liquid spawn of 10ml in every 100mg cellulosic mixture.
3) Dioscorea. zingiberensis Wright Starch liquefying-saccharifying: the Dioscorea. zingiberensis Wright Starch after washing is added to supernatant, making solid content in starch milk is 8%, its pH value is adjusted to 6.2, and is warming up to 80 DEG C, then add AMS and calcium chloride, and be incubated enzymolysis 1.5h, then cool the temperature to 58 DEG C, then pH value is adjusted to 5.5, and add carbohydrase and Pullulanase, insulation enzymolysis 3h, obtains Dioscorea. zingiberensis Wright Starch enzymolysis liquid; Concrete, adding after AMS and calcium chloride, the vigor of corresponding every gram of starch AMS is 240U, the Ca in every kilogram of starch
2+ion concentration is 240mg; Add after carbohydrase and Pullulanase, the vigor of corresponding every gram of turmeric starch saccharification enzyme is 300U, and the vigor of Pullulanase is 40U.
4) probio protein feed preparation: Dioscorea. zingiberensis Wright Starch enzymolysis liquid is cooled to after 45 DEG C, adds (NH
4)
2sO
4, peptone, anhydrous sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, stir and regulate pH value to 6.0 to obtain mixed enzymolysis liquid, access again Paula enlightening yeast and lactobacillus acidophilus, 37 DEG C of bottom fermentation 60h, after will yellow ginger fibrous residue adding, mix, and low temperature drying obtains yellow ginger probio albumen and raises.Concrete, add (the NH of 10g by every liter of Dioscorea. zingiberensis Wright Starch enzymolysis liquid
4)
2sO
4, the peptone of 5.0g, the anhydrous sodium acetate of 3.0g, the diammonium hydrogen citrate of 6.0g, the Tween 80 of 1.0mL, the magnesium sulfate of 0.3g, the manganese sulfate of 0.06g, the dipotassium hydrogen phosphate of 4.0g; In every 100ml mixed enzymolysis liquid, access respectively in proportion Paula lactobacillus acidophilus bacterial classification enlightening yeast and 6ml of 4ml.
Example 2
Utilize yellow ginger to produce a method for probio protein feed, comprise the steps,
1) yellow ginger cellulosic and starch separate: after yellow ginger is cleaned to pulverizing, filtration obtains cellulosic; To leach thing and leave standstill and be divided into three layers, upper strata is supernatant, and centre be suspension, and lower floor is starch, pours out supernatant for subsequent use, isolates lower floor's starch and also washs; Preferably, yellow ginger adopts the cadmium yellow ginger of water content 66%; Concrete employing 80 object filter-cloth filterings obtain cellulosic, leach after thing leaves standstill 5h and are divided into three layers.
2) yellow ginger cellulosic fermentation: add wheat bran, ammonium sulfate and Tween 80 in yellow ginger cellulosic, and stir and obtain cellulosic mixture, then sterilizing is cooled to 35 DEG C of access Trichoderma virides, after 30 DEG C of constant temperature culture 4d, obtains the yellow ginger fibrous residue of cellulase and mycoprotein; Concrete, in yellow ginger cellulosic, in every kilogram of yellow ginger cellulosic, add respectively in proportion the Tween 80 of the wheat bran of 25g and the ammonium sulfate of 20g and 1.2ml; Sterilizing 20min at the temperature of 121 DEG C, is cooled to after 35 DEG C, accesses in proportion the Trichoderma viride liquid spawn of 10ml in every 100mg cellulosic mixture.
3) Dioscorea. zingiberensis Wright Starch liquefying-saccharifying: the Dioscorea. zingiberensis Wright Starch after washing is added to supernatant, making solid content in starch milk is 9%, its pH value is adjusted to 6.3, and is warming up to 75 DEG C, then add AMS and calcium chloride, and be incubated enzymolysis 2h, then cool the temperature to 60 DEG C, then pH value is adjusted to 5.8, and add carbohydrase and Pullulanase, insulation enzymolysis 4h, obtains Dioscorea. zingiberensis Wright Starch enzymolysis liquid; Concrete, adding after AMS and calcium chloride, the vigor of corresponding every gram of starch AMS is 220U, the Ca in every kilogram of starch
2+ion concentration is 220mg; Add after carbohydrase and Pullulanase, the vigor of corresponding every gram of turmeric starch saccharification enzyme is 240U, and the vigor of Pullulanase is 20U.
4) probio protein feed preparation: Dioscorea. zingiberensis Wright Starch enzymolysis liquid is cooled to after 45 DEG C, adds (NH
4)
2sO
4, peptone, anhydrous sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, stir and regulate pH value to 5.5 to obtain mixed enzymolysis liquid, access again Paula enlightening yeast and lactobacillus acidophilus, 37 DEG C of bottom fermentation 48h, after will yellow ginger fibrous residue adding, mix, and low temperature drying obtains yellow ginger probio albumen and raises.Concrete, add (the NH of 8.0g by every liter of Dioscorea. zingiberensis Wright Starch enzymolysis liquid
4)
2sO
4, the peptone of 4.0g, the anhydrous sodium acetate of 2.0g, the diammonium hydrogen citrate of 5.0g, the Tween 80 of 0.8mL, the magnesium sulfate of 0.2g, the manganese sulfate of 0.04g, the dipotassium hydrogen phosphate of 2.0g; In every 100ml mixed enzymolysis liquid, access respectively in proportion Paula lactobacillus acidophilus bacterial classification enlightening yeast and 8ml of 6ml.
Example 3
Utilize yellow ginger to produce a method for probio protein feed, comprise the steps,
1) yellow ginger cellulosic and starch separate: after yellow ginger is cleaned to pulverizing, filtration obtains cellulosic; To leach thing and leave standstill and be divided into three layers, upper strata is supernatant, and centre be suspension, and lower floor is starch, pours out supernatant for subsequent use, isolates lower floor's starch and also washs; Preferably, yellow ginger adopts the cadmium yellow ginger of water content 67%; Concrete employing 90 object filter-cloth filterings obtain cellulosic, leach after thing leaves standstill 6h and are divided into three layers.
2) yellow ginger cellulosic fermentation: add wheat bran, ammonium sulfate and Tween 80 in yellow ginger cellulosic, and stir and obtain cellulosic mixture, then sterilizing is cooled to 40 DEG C of access Trichoderma virides, after 33 DEG C of constant temperature culture 3d, obtains the yellow ginger fibrous residue of cellulase and mycoprotein; Concrete, in yellow ginger cellulosic, in every kilogram of yellow ginger cellulosic, add respectively in proportion the Tween 80 of the wheat bran of 21g and the ammonium sulfate of 12g and 0.9ml; Sterilizing 25min at the temperature of 121 DEG C, is cooled to after 40 DEG C, accesses in proportion the Trichoderma viride liquid spawn of 7ml in every 100mg cellulosic mixture.
3) Dioscorea. zingiberensis Wright Starch liquefying-saccharifying: the Dioscorea. zingiberensis Wright Starch after washing is added to supernatant, making solid content in starch milk is 10%, its pH value is adjusted to 6.4, and is warming up to 70 DEG C, then add AMS and calcium chloride, and be incubated enzymolysis 1h, then cool the temperature to 55 DEG C, then pH value is adjusted to 5.4, and add carbohydrase and Pullulanase, insulation enzymolysis 3.5h, obtains Dioscorea. zingiberensis Wright Starch enzymolysis liquid; Concrete, adding after AMS and calcium chloride, the vigor of corresponding every gram of starch AMS is 210U, the Ca in every kilogram of starch
2+ion concentration is 230mg; Add after carbohydrase and Pullulanase, the vigor of corresponding every gram of turmeric starch saccharification enzyme is 250U, and the vigor of Pullulanase is 30U.
4) probio protein feed preparation: Dioscorea. zingiberensis Wright Starch enzymolysis liquid is cooled to after 50 DEG C, adds (NH
4)
2sO
4, peptone, anhydrous sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, stir and regulate pH value to 5.0 to obtain mixed enzymolysis liquid, access again Paula enlightening yeast and lactobacillus acidophilus, 34 DEG C of bottom fermentation 50h, after will yellow ginger fibrous residue adding, mix, and low temperature drying obtains yellow ginger probio albumen and raises.Concrete, add (the NH of 9.0g by every liter of Dioscorea. zingiberensis Wright Starch enzymolysis liquid
4)
2sO
4, the peptone of 4.5g, the anhydrous sodium acetate of 2.5g, the diammonium hydrogen citrate of 5.5g, the Tween 80 of 0.9mL, the magnesium sulfate of 0.2g, the manganese sulfate of 0.04g, the dipotassium hydrogen phosphate of 2.0g; In every 100ml mixed enzymolysis liquid, access respectively in proportion Paula lactobacillus acidophilus bacterial classification enlightening yeast and 8ml of 6ml.
Example 4
Utilize yellow ginger to produce a method for probio protein feed, comprise the steps,
1) yellow ginger cellulosic and starch separate: after yellow ginger is cleaned to pulverizing, filtration obtains cellulosic; To leach thing and leave standstill and be divided into three layers, upper strata is supernatant, and centre be suspension, and lower floor is starch, pours out supernatant for subsequent use, isolates lower floor's starch and also washs; Preferably, yellow ginger adopts the cadmium yellow ginger of water content 68%; Concrete employing 85 object filter-cloth filterings obtain cellulosic, leach after thing leaves standstill 5h and are divided into three layers.
2) yellow ginger cellulosic fermentation: add wheat bran, ammonium sulfate and Tween 80 in yellow ginger cellulosic, and stir and obtain cellulosic mixture, then sterilizing is cooled to 36 DEG C of access Trichoderma virides, after 27 DEG C of constant temperature culture 4d, obtains the yellow ginger fibrous residue of cellulase and mycoprotein; Concrete, in yellow ginger cellulosic, in every kilogram of yellow ginger cellulosic, add respectively in proportion the Tween 80 of the wheat bran of 22g and the ammonium sulfate of 14g and 1.0ml; Sterilizing 21min at the temperature of 121 DEG C, is cooled to after 36 DEG C, accesses in proportion the Trichoderma viride liquid spawn of 6ml in every 100mg cellulosic mixture.
3) Dioscorea. zingiberensis Wright Starch liquefying-saccharifying: the Dioscorea. zingiberensis Wright Starch after washing is added to supernatant, making solid content in starch milk is 11%, its pH value is adjusted to 6.5, and is warming up to 72 DEG C, then add AMS and calcium chloride, and be incubated enzymolysis 1.8h, then cool the temperature to 56 DEG C, then pH value is adjusted to 6.0, and add carbohydrase and Pullulanase, insulation enzymolysis 3h, obtains Dioscorea. zingiberensis Wright Starch enzymolysis liquid; Concrete, adding after AMS and calcium chloride, the vigor of corresponding every gram of starch AMS is 240U, the Ca in every kilogram of starch
2+ion concentration is 210mg; Add after carbohydrase and Pullulanase, the vigor of corresponding every gram of turmeric starch saccharification enzyme is 270U, and the vigor of Pullulanase is 25U.
4) probio protein feed preparation: Dioscorea. zingiberensis Wright Starch enzymolysis liquid is cooled to after 48 DEG C, adds (NH
4)
2sO
4, peptone, anhydrous sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, stir and regulate pH value to 6.0 to obtain mixed enzymolysis liquid, access again Paula enlightening yeast and lactobacillus acidophilus, 39 DEG C of bottom fermentation 55h, after will yellow ginger fibrous residue adding, mix, and low temperature drying obtains yellow ginger probio albumen and raises.Concrete, add (the NH of 8.5g by every liter of Dioscorea. zingiberensis Wright Starch enzymolysis liquid
4)
2sO
4, the peptone of 4.0g, the anhydrous sodium acetate of 3.0g, the diammonium hydrogen citrate of 5.0g, the Tween 80 of 1.0mL, the magnesium sulfate of 0.2g, the manganese sulfate of 0.06g, the dipotassium hydrogen phosphate of 4.0g; In every 100ml mixed enzymolysis liquid, access respectively in proportion Paula lactobacillus acidophilus bacterial classification enlightening yeast and 8ml of 4ml.
Example 5
Utilize yellow ginger to produce a method for probio protein feed, comprise the steps,
1) yellow ginger cellulosic and starch separate: after yellow ginger is cleaned to pulverizing, filtration obtains cellulosic; To leach thing and leave standstill and be divided into three layers, upper strata is supernatant, and centre be suspension, and lower floor is starch, pours out supernatant for subsequent use, isolates lower floor's starch and also washs; Preferably, yellow ginger adopts the cadmium yellow ginger of water content 70%; Concrete employing 95 object filter-cloth filterings obtain cellulosic, leach after thing leaves standstill 6h and are divided into three layers.
2) yellow ginger cellulosic fermentation: add wheat bran, ammonium sulfate and Tween 80 in yellow ginger cellulosic, and stir and obtain cellulosic mixture, then sterilizing is cooled to 38 DEG C of access Trichoderma virides, after 31 DEG C of constant temperature culture 3d, obtains the yellow ginger fibrous residue of cellulase and mycoprotein; Concrete, in yellow ginger cellulosic, in every kilogram of yellow ginger cellulosic, add respectively in proportion the Tween 80 of the wheat bran of 24g and the ammonium sulfate of 16g and 1.1ml; Sterilizing 23min at the temperature of 121 DEG C, is cooled to after 37 DEG C, accesses in proportion the Trichoderma viride liquid spawn of 9ml in every 100mg cellulosic mixture.
3) Dioscorea. zingiberensis Wright Starch liquefying-saccharifying: the Dioscorea. zingiberensis Wright Starch after washing is added to supernatant, making solid content in starch milk is 12%, its pH value is adjusted to 6.2, and is warming up to 78 DEG C, then add AMS and calcium chloride, and be incubated enzymolysis 1.4h, then cool the temperature to 59 DEG C, then pH value is adjusted to 5.7, and add carbohydrase and Pullulanase, insulation enzymolysis 4h, obtains Dioscorea. zingiberensis Wright Starch enzymolysis liquid; Concrete, adding after AMS and calcium chloride, the vigor of corresponding every gram of starch AMS is 230U, the Ca in every kilogram of starch
2+ion concentration is 240mg; Add after carbohydrase and Pullulanase, the vigor of corresponding every gram of turmeric starch saccharification enzyme is 290U, and the vigor of Pullulanase is 35U.
4) probio protein feed preparation: Dioscorea. zingiberensis Wright Starch enzymolysis liquid is cooled to after 46 DEG C, adds (NH
4)
2sO
4, peptone, anhydrous sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, stir and regulate pH value to 5.0 to obtain mixed enzymolysis liquid, access again Paula enlightening yeast and lactobacillus acidophilus, 35 DEG C of bottom fermentation 57h, after will yellow ginger fibrous residue adding, mix, and low temperature drying obtains yellow ginger probio albumen and raises.Concrete, add (the NH of 9.5g by every liter of Dioscorea. zingiberensis Wright Starch enzymolysis liquid
4)
2sO
4, the peptone of 5.0g, the anhydrous sodium acetate of 2.0g, the diammonium hydrogen citrate of 6.0g, the Tween 80 of 0.8mL, the magnesium sulfate of 0.3g, the manganese sulfate of 0.04g, the dipotassium hydrogen phosphate of 2.0g; In every 100ml mixed enzymolysis liquid, access respectively in proportion Paula lactobacillus acidophilus bacterial classification enlightening yeast and 6ml of 6ml.
Claims (4)
1. utilize yellow ginger to produce a method for probio protein feed, it is characterized in that, comprise the steps,
1) yellow ginger cellulosic and starch separate: after yellow ginger is cleaned to pulverizing, filtration obtains cellulosic; To leach thing and leave standstill and be divided into three layers, upper strata is supernatant, and centre be suspension, and lower floor is starch, pours out supernatant for subsequent use, isolates lower floor's starch and also washs;
2) yellow ginger cellulosic fermentation: the wheat bran, the ammonium sulfate of 10-20g and the Tween 80 of 0.8-1.2ml that add respectively in proportion 20-25g in yellow ginger cellulosic in every kilogram of yellow ginger cellulosic, and stir and obtain cellulosic mixture, then sterilizing 20min-25min at the temperature of 121 DEG C, be cooled to after 35-40 DEG C, in every 100mg cellulosic mixture, access in proportion the Trichoderma viride liquid spawn of 6-10ml, after 27-33 DEG C of constant temperature culture 3-4d, obtain the yellow ginger fibrous residue of cellulase and mycoprotein;
3) Dioscorea. zingiberensis Wright Starch liquefying-saccharifying: the Dioscorea. zingiberensis Wright Starch after washing is added to supernatant, making solid content in starch milk is 8-12%, its pH value is adjusted to 6.2-6.5, and be warming up to 70-80 DEG C, add again AMS and calcium chloride, the vigor of corresponding every gram of starch AMS is 200-240U, the Ca in every kilogram of starch
2+ion concentration is 200-240mg; And be incubated enzymolysis 1-2h, and then cooling the temperature to 55-60 DEG C, then pH value is adjusted to 5.4-6.0, and add carbohydrase and Pullulanase, the vigor of corresponding every gram of turmeric starch saccharification enzyme is 240-300U, the vigor of Pullulanase is 20-40U; Insulation enzymolysis 3-4h, obtains Dioscorea. zingiberensis Wright Starch enzymolysis liquid;
4) probio protein feed preparation: Dioscorea. zingiberensis Wright Starch enzymolysis liquid is cooled to after 45-50 DEG C, adds (the NH of 8-10g by every liter of Dioscorea. zingiberensis Wright Starch enzymolysis liquid
4)
2sO
4, the peptone of 4-5g, the anhydrous sodium acetate of 2-3.0g, the diammonium hydrogen citrate of 5-6g, the Tween 80 of 0.8-1.0mL, the magnesium sulfate of 0.2-0.3g, the manganese sulfate of 0.04-0.06g, the dipotassium hydrogen phosphate of 2.0-4.0g, stir and regulate pH value to obtain mixed enzymolysis liquid to 5.0-6.0, access respectively in proportion again Paula lactobacillus acidophilus bacterial classification enlightening yeast and 6-8ml of 4-6ml in every 100ml mixed enzymolysis liquid, 34-39 DEG C of bottom fermentation 48-60h, after yellow ginger fibrous residue is added, mix, and low temperature drying obtains yellow ginger probio protein feed.
2. a kind of method of utilizing yellow ginger to produce probio protein feed according to claim 1, is characterized in that step 1) in, yellow ginger adopts the cadmium yellow ginger of water content 65-70%.
3. a kind of method of utilizing yellow ginger to produce probio protein feed according to claim 1, is characterized in that step 1) in, adopt 80-100 object filter-cloth filtering to obtain cellulosic, leach after thing leaves standstill 5-6h and be divided into three layers.
4. a probio protein feed that utilizes yellow ginger to produce, is characterized in that, is made by the method for utilizing yellow ginger to produce probio protein feed described in any one in claim 1-3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310422512.2A CN103478410B (en) | 2013-09-16 | 2013-09-16 | Probiotic protein feed produced through peltate yam rhizomes and production method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310422512.2A CN103478410B (en) | 2013-09-16 | 2013-09-16 | Probiotic protein feed produced through peltate yam rhizomes and production method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103478410A CN103478410A (en) | 2014-01-01 |
| CN103478410B true CN103478410B (en) | 2014-08-13 |
Family
ID=49819273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310422512.2A Active CN103478410B (en) | 2013-09-16 | 2013-09-16 | Probiotic protein feed produced through peltate yam rhizomes and production method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103478410B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104073542B (en) * | 2014-06-24 | 2017-08-29 | 绍兴文理学院 | It is a kind of to combine the method that microorganism solid fermentation method produces Chinese yam saponin |
| CN108220191A (en) * | 2017-12-30 | 2018-06-29 | 陕西科技大学 | A kind of probiotics and preparation method and application |
| CN108013261A (en) * | 2017-12-30 | 2018-05-11 | 陕西石羊农业科技股份有限公司 | One breeder probiotics feed and preparation method thereof |
| CN108244337A (en) * | 2017-12-30 | 2018-07-06 | 陕西石羊农业科技股份有限公司 | One boar probiotics feed and preparation method thereof |
| CN108740430A (en) * | 2018-06-15 | 2018-11-06 | 旬阳县宏达化工有限公司 | Pannage and method made of a kind of ginger saponin extraction waste residue |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1579201A (en) * | 2004-05-17 | 2005-02-16 | 陕西省科学院酶工程研究所 | Feed additive containing microbe and biological enzyme |
| CN101705275A (en) * | 2009-10-30 | 2010-05-12 | 陕西科技大学 | Process for producing diosgenin and method for processing peltate yam after extracting same |
| CN101816372A (en) * | 2010-05-17 | 2010-09-01 | 湘西自治州奥瑞克医药化工有限责任公司 | Clean production method of coproduced biologic protein feed of diosgenin |
| CN102599351A (en) * | 2012-04-16 | 2012-07-25 | 辽宁神农生物工程有限公司 | Bio-enzyme and microorganism containing compound feed additive and preparation process thereof |
| CN102870956A (en) * | 2012-09-14 | 2013-01-16 | 董爱文 | Method for preparing feed by enzymolysis of ginger cauline leaves |
| CN103060416A (en) * | 2012-12-20 | 2013-04-24 | 华中科技大学 | Method for cleaning and producing dioscorea zingiberensis saponin with microbial technology adopted |
-
2013
- 2013-09-16 CN CN201310422512.2A patent/CN103478410B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1579201A (en) * | 2004-05-17 | 2005-02-16 | 陕西省科学院酶工程研究所 | Feed additive containing microbe and biological enzyme |
| CN101705275A (en) * | 2009-10-30 | 2010-05-12 | 陕西科技大学 | Process for producing diosgenin and method for processing peltate yam after extracting same |
| CN101816372A (en) * | 2010-05-17 | 2010-09-01 | 湘西自治州奥瑞克医药化工有限责任公司 | Clean production method of coproduced biologic protein feed of diosgenin |
| CN102599351A (en) * | 2012-04-16 | 2012-07-25 | 辽宁神农生物工程有限公司 | Bio-enzyme and microorganism containing compound feed additive and preparation process thereof |
| CN102870956A (en) * | 2012-09-14 | 2013-01-16 | 董爱文 | Method for preparing feed by enzymolysis of ginger cauline leaves |
| CN103060416A (en) * | 2012-12-20 | 2013-04-24 | 华中科技大学 | Method for cleaning and producing dioscorea zingiberensis saponin with microbial technology adopted |
Non-Patent Citations (6)
| Title |
|---|
| 伍新花等.黄姜纤维素渣固态发酵生产蛋白饲料.《环境工程学报》.2012,第6卷(第5期),第1734-1738页. |
| 周旋等.黄姜皂素工业固体废弃物的综合利用.《武汉工程大学学报》.2012,第34卷(第8期),第54-57页. |
| 徐徽等.黄姜提取皂素过程中淀粉的回收与利用.《化学工程师》.2005,(第8期),第7-9页. |
| 黄姜提取皂素过程中淀粉的回收与利用;徐徽等;《化学工程师》;20050831(第8期);第7-9页 * |
| 黄姜皂素工业固体废弃物的综合利用;周旋等;《武汉工程大学学报》;20120831;第34卷(第8期);第54-57页 * |
| 黄姜纤维素渣固态发酵生产蛋白饲料;伍新花等;《环境工程学报》;20120531;第6卷(第5期);第1734-1738页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103478410A (en) | 2014-01-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103478410B (en) | Probiotic protein feed produced through peltate yam rhizomes and production method thereof | |
| CN102876590B (en) | Penicillium sp. mutant strain and application of penicillium sp. mutant strain to cellulase preparation | |
| CN104694587B (en) | A kind of method that lactic acid is produced by bagasse | |
| CN105256081B (en) | A method of xylose is prepared using spirit stillage | |
| CN108070630A (en) | A kind of preparation method of high-purity Lins eed protein | |
| CN103981223A (en) | Method for preparing ethanol by fermenting manioc waste | |
| WO2016173262A1 (en) | Method for producing cellulosic ethanol by fermenting agricultural and forest biomass waste thick mash | |
| CN103497987B (en) | Clean production method of yam diosgenin | |
| CN102618609B (en) | Method for comprehensively utilizing degreased rice bran | |
| CN110004196B (en) | Process for co-producing fuel ethanol and DDGS by using straw and corn | |
| CN103478409B (en) | Protein feed produced through peltate yam rhizomes and production method thereof | |
| CN105907801B (en) | Utilize the method for potato residues continuous production dietary fiber, alcohol and single cell protein | |
| CN106755249A (en) | A kind of method of degreasing high-temperature rice bran dregs of rice comprehensive utilization | |
| CN106974268A (en) | A kind of preparation method of black fruit fructus lycii ferment | |
| CN101735331B (en) | Production process for extracting lily polysaccharides through fermentation method and product thereof | |
| CN102894182A (en) | Method for producing biological feed by utilizing fermentation of dwarf lilyturf tuber dregs | |
| CN104651416A (en) | Method for synchronously producing biological butanol and nano cellulose from sweet sorghum | |
| CN101579039B (en) | Method for producing animal feed from oil-tea-cake dregs fermented by neurospora crassa | |
| CN105400754A (en) | High-valued comprehensive utilizing method for traditional Chinese medicine residues rich in cellulose matter | |
| CN106834407B (en) | Method for green production of diosgenin by biological method | |
| CN105177089A (en) | Method for increasing content of chitin in mycelium residues by means of secondary fermentation | |
| CN105368912B (en) | A method of spraying counter-current extracts Sodium Hyaluronate | |
| CN107502636A (en) | A kind of method using ammoniacal liquor Cold pretreatment hybrid Chinese pennisetum | |
| CN103478408B (en) | Yeast protein active feed produced through peltate yam rhizome starch and method thereof | |
| CN103409352B (en) | Compound microbial community for preparing feed by fermenting soybean hulls |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210907 Address after: 201807 floor 2, building 1, No. 1104, Jiatang Road, Jiading Industrial Zone, Jiading District, Shanghai Patentee after: SHANGHAI LIKANG BIOLOGICAL HIGH-TECH Co.,Ltd. Address before: 300110 1-1-505-1, Xindu building, southwest of the intersection of Weijin road and wandezhuang street, Nankai District, Tianjin Patentee before: HUIJIAWANG (TIANJIN) TECHNOLOGY Co.,Ltd. Effective date of registration: 20210907 Address after: 300110 1-1-505-1, Xindu building, southwest of the intersection of Weijin road and wandezhuang street, Nankai District, Tianjin Patentee after: HUIJIAWANG (TIANJIN) TECHNOLOGY Co.,Ltd. Address before: No. 1, Weiyang District university garden, Xi'an, Shaanxi Province, Shaanxi Patentee before: SHAANXI University OF SCIENCE & TECHNOLOGY |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221025 Address after: 200436 Room 302, Building 3, No. 39, Lane 1861, Hutai Road, Baoshan District, Shanghai Patentee after: Maidu Chongyi (Shanghai) Technology Development Co.,Ltd. Address before: 201807 floor 2, building 1, No. 1104, Jiatang Road, Jiading Industrial Zone, Jiading District, Shanghai Patentee before: SHANGHAI LIKANG BIOLOGICAL HIGH-TECH Co.,Ltd. |
|
| TR01 | Transfer of patent right |