CN108220191A - A kind of probiotics and preparation method and application - Google Patents
A kind of probiotics and preparation method and application Download PDFInfo
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- CN108220191A CN108220191A CN201711487642.9A CN201711487642A CN108220191A CN 108220191 A CN108220191 A CN 108220191A CN 201711487642 A CN201711487642 A CN 201711487642A CN 108220191 A CN108220191 A CN 108220191A
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- China
- Prior art keywords
- cfu
- bacterium powder
- probiotics
- lactobacillus acidophilus
- paula
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- 239000006041 probiotic Substances 0.000 title claims abstract description 43
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 239000000843 powder Substances 0.000 claims abstract description 64
- 241000894006 Bacteria Species 0.000 claims abstract description 53
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 45
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 36
- 150000001875 compounds Chemical class 0.000 claims abstract description 35
- 241000287828 Gallus gallus Species 0.000 claims abstract description 8
- 241001494479 Pecora Species 0.000 claims abstract description 8
- 235000021255 galacto-oligosaccharides Nutrition 0.000 claims abstract description 8
- 150000003271 galactooligosaccharides Chemical class 0.000 claims abstract description 8
- 108010011619 6-Phytase Proteins 0.000 claims abstract description 7
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 7
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 7
- 229940085127 phytase Drugs 0.000 claims abstract description 7
- 240000006365 Vitis vinifera Species 0.000 claims description 28
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 28
- 235000012907 honey Nutrition 0.000 claims description 28
- 238000004108 freeze drying Methods 0.000 claims description 26
- MUKYLHIZBOASDM-UHFFFAOYSA-N 2-[carbamimidoyl(methyl)amino]acetic acid 2,3,4,5,6-pentahydroxyhexanoic acid Chemical compound NC(=N)N(C)CC(O)=O.OCC(O)C(O)C(O)C(O)C(O)=O MUKYLHIZBOASDM-UHFFFAOYSA-N 0.000 claims description 20
- 241001533104 Tribulus terrestris Species 0.000 claims description 20
- 239000002002 slurry Substances 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 238000005119 centrifugation Methods 0.000 claims description 13
- 241000235342 Saccharomycetes Species 0.000 claims description 12
- 239000012153 distilled water Substances 0.000 claims description 12
- 239000008363 phosphate buffer Substances 0.000 claims description 12
- 238000011534 incubation Methods 0.000 claims description 11
- 235000008586 Hovenia Nutrition 0.000 claims description 10
- 241000405398 Hovenia Species 0.000 claims description 10
- 150000004676 glycans Chemical class 0.000 claims description 10
- 229920001282 polysaccharide Polymers 0.000 claims description 10
- 239000005017 polysaccharide Substances 0.000 claims description 10
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000000047 product Substances 0.000 claims description 7
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 6
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 6
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 6
- 235000019743 Choline chloride Nutrition 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 235000010955 Hovenia acerba Nutrition 0.000 claims description 6
- 241001533134 Hovenia acerba Species 0.000 claims description 6
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 6
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 6
- 239000004473 Threonine Substances 0.000 claims description 6
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 6
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 6
- KLOIYEQEVSIOOO-UHFFFAOYSA-N carbocromen Chemical compound CC1=C(CCN(CC)CC)C(=O)OC2=CC(OCC(=O)OCC)=CC=C21 KLOIYEQEVSIOOO-UHFFFAOYSA-N 0.000 claims description 6
- 229940106157 cellulase Drugs 0.000 claims description 6
- 229960003178 choline chloride Drugs 0.000 claims description 6
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 229940088598 enzyme Drugs 0.000 claims description 6
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 229940059442 hemicellulase Drugs 0.000 claims description 6
- 108010002430 hemicellulase Proteins 0.000 claims description 6
- 235000017281 sodium acetate Nutrition 0.000 claims description 6
- 239000001632 sodium acetate Substances 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 5
- 235000010469 Glycine max Nutrition 0.000 claims description 5
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 5
- 108010079058 casein hydrolysate Proteins 0.000 claims description 5
- 229930182817 methionine Natural products 0.000 claims description 5
- 235000009508 confectionery Nutrition 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 229920001277 pectin Polymers 0.000 claims description 3
- 235000010987 pectin Nutrition 0.000 claims description 3
- 239000001814 pectin Substances 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 239000004472 Lysine Substances 0.000 claims description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 2
- 238000009835 boiling Methods 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims description 2
- 239000008367 deionised water Substances 0.000 claims description 2
- 229910021641 deionized water Inorganic materials 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 235000018102 proteins Nutrition 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 238000000967 suction filtration Methods 0.000 claims description 2
- 239000006228 supernatant Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 claims 2
- FIMJSWFMQJGVAM-UHFFFAOYSA-N chloroform;hydrate Chemical compound O.ClC(Cl)Cl FIMJSWFMQJGVAM-UHFFFAOYSA-N 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 244000144972 livestock Species 0.000 abstract description 11
- 244000144977 poultry Species 0.000 abstract description 9
- 206010012735 Diarrhoea Diseases 0.000 abstract description 2
- 208000035240 Disease Resistance Diseases 0.000 abstract description 2
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 abstract description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 2
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 244000005709 gut microbiome Species 0.000 abstract description 2
- 230000000968 intestinal effect Effects 0.000 abstract description 2
- CUXQLKLUPGTTKL-UHFFFAOYSA-M microcosmic salt Chemical compound [NH4+].[Na+].OP([O-])([O-])=O CUXQLKLUPGTTKL-UHFFFAOYSA-M 0.000 abstract description 2
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 2
- 239000011574 phosphorus Substances 0.000 abstract description 2
- 235000002949 phytic acid Nutrition 0.000 abstract description 2
- 229940068041 phytic acid Drugs 0.000 abstract description 2
- 239000000467 phytic acid Substances 0.000 abstract description 2
- 230000002265 prevention Effects 0.000 abstract description 2
- 230000035755 proliferation Effects 0.000 abstract description 2
- 241001465754 Metazoa Species 0.000 description 6
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000003115 biocidal effect Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000003223 protective agent Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 238000005660 chlorination reaction Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 108010076119 Caseins Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
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- Engineering & Computer Science (AREA)
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- Microbiology (AREA)
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- Wood Science & Technology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
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- Polymers & Plastics (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Food Science & Technology (AREA)
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- Fodder In General (AREA)
Abstract
A kind of probiotics and preparation method and application, including single lactobacillus acidophilus bacterium powder, Paula enlightening yeast bacterium powder or both compound micro-ecological preparation；Probiotics are added to be uniformly mixed in pig, ox, sheep, chicken animal and fowl fodder by the present invention by 0.05% 0.2% mass ratio is made probiotics feed.Lactobacillus acidophilus and Paula enlightening yeast can improve livestock and poultry intestinal microflora equilibrium and prevention is had loose bowels in probiotics of the present invention, the addition of synanthrin and galactooligosaccharide promotes livestock and poultry to the absorption of nutriment and promotes proliferation of intestinal probiotics, the addition of phytase increases utilization rate of the livestock and poultry to phytic acid in feed, reduce the utilization rate of microcosmic salt and the discharge of phosphorus, the disease resistance and the Utilization ability to feed, the speed of growth that the probiotics of the present invention increase livestock and poultry are fast.
Description
Technical field
The invention belongs to probiotics industrial technical fields, and in particular to a kind of probiotics and preparation method thereof with
Using.
Background technology
Antibiotic animal husbandry field application, to promote animal husbandry scale development and modernization cultivation play it is huge
Impetus however, due to the abuse of antibiotic, produces various side effects, and particularly antibiotic is residual in animal products
It stays, directly threatens the health of the mankind, cause the potential hazard of livestock products.From in January, 2006 so far, European Union completely forbids anti-
Raw element is used for animal feed.China have every year export to Japan, the U.S., European Union animal products given with various technical barriers
Retract or destroy with limitation, drug residue is exceeded be influence China's animal products quality main problem and I
State's livestock products enter the major obstacle of international market.In view of the foregoing, for abuse of antibiotics drug is avoided to cause drug residual
The potential hazard stayed, exploitation have effects that the probiotics for preventing disease and feed are very urgent.Since probiotics have
Functional more, the advantages that having no toxic side effect, do not remain, is free from environmental pollution, it has also become ideal indirect substitute antibiotics medicine
One of object and the product with diseases prevention, therefore it is necessary as feed addictive to develop probiotics.
Invention content
The purpose of the present invention is to provide a kind of single and compound probiotics feed and preparation method and application,
To improve the production performance of livestock and poultry and the general level of the health, improve efficiency of feed utilization.
In order to achieve the above object, probiotics of the invention, including single lactobacillus acidophilus bacterium powder, Paula enlightening yeast
Bacterium powder or both compound micro-ecological preparation；
Described single its viable count of lactobacillus acidophilus bacterium powder is 2.1 × 1011CFU/g-6.0×1011CFU/g；
The single Paula enlightening yeast bacterium powder, viable count are 3.0 × 1010CFU/g-5.0×1010CFU/g；
The compound micro-ecological preparation presses 2 by Paula enlightening yeast bacterium powder and lactobacillus acidophilus bacterium powder:1~1:2 quality
Than composition, the wherein viable count of Paula enlightening saccharomycete bacterium powder is 1.0 × 1010CFU/g-2.5×1010CFU/g, lactobacillus acidophilus
Bacterium powder viable count is 1.0 × 1011CFU/g-3.0×1011CFU/g。
The preparation method of the probiotics of the present invention is as follows：
It is prepared by single Paula enlightening yeast bacterium powder：
Fresh honey raisin tree is taken to be beaten to obtain honey raisin tree slurry after cleaning, is separately added into honey raisin tree slurry quality 0.5-1%'s into honey raisin tree slurry
Pectase, cellulase and hemicellulase keep the temperature 2-3h in 60 DEG C, then filter and obtain hovenia acerba juice, after being cooled to 38-40 DEG C
The Paula enlightening yeast activated of 1-5% is accessed by volume, after 37 DEG C of constant temperature incubation 28-36h, at 4-8 DEG C, 4000-
6000rpm centrifugations 10-20min collects Paula enlightening yeast bacterium mud, then adds the compound freeze drying protectant postposition of 2 times of weight of bacterium mud
Be freeze-dried in vacuum freeze drier is for 24 hours 3.0 × 10 to get viable count10CFU/g-5.0×1010The Paula of CFU/g
Enlightening saccharomycete bacterium powder；
The 100g compound freeze drying protectant 10-15g containing synanthrin, trehalose 8-12g, threonine 0.4-0.8g, egg ammonia
Sour 2-5g, sodium chloride 2-2.8g, phosphate buffer 20-25g, distilled water 42.9-57.6g；
It is prepared by single lactobacillus acidophilus bacterium powder：
The glucose of puncture vine extracting solution quality 1-2%, the casein hydrolysis of 0.2-0.4% are added in puncture vine extracting solution
Then the diammonium hydrogen citrate of object, the sodium acetate of 0.1-0.3% and 0.2-0.4% accesses acidophilus breast bar by the volume ratio of 2-5%
Bacterium, after 37 DEG C of constant temperature incubation 18-24h, in 4-8 DEG C, 6000-8000rpm centrifugations 10-20min obtains lactobacillus acidophilus bacterium mud,
Then the compound freeze drying protectant of 1-3 times of addition bacterium mud weight is placed on vacuum freeze drier and dries is for 24 hours to get viable count
2.1×1011CFU/g-6.0×1011The lactobacillus acidophilus bacterium powder of CFU/g；
Every 100g compound freeze drying protectant 2-5g containing phytase, the galactooligosaccharide 10-15g, hovenia polysaccharides 3-5g, beans
Oily 1-3g, lysine 1-3g, choline chloride 3-5g, phosphate buffer 20-25g, distilled water 41-60g；
It is prepared by compound micro-ecological preparation：
Single Paula enlightening yeast bacterium powder and single lactobacillus acidophilus bacterium powder are pressed 2:1~1:2 mass ratio mixing, wherein protecting
The viable count of Lardy saccharomycete bacterium powder is 1.0 × 1010CFU/g-2.5×1010CFU/g, lactobacillus acidophilus bacterium powder viable count are
1.0×1011CFU/g-3.0×1011CFU/g。
The preparation method of the puncture vine extracting solution is：Puncture vine is taken by 1：14 mass ratio soaks 25min, then heats
It boils and maintains boiling 30min, then with 400 mesh screens, adding deionized water afterwards twice with vacuum pump suction filtration after cooling makes
Puncture vine and water ratio are 1:10 up to puncture vine extracting solution.
The preparation method of the hovenia polysaccharides is：The supernatant after the enlightening yeast bacterium mud of Paula will be collected by centrifugation to concentrate through low temperature
The absolute ethyl alcohol of 3 times of volumes is added in after to 1/3,3h is stood and filters to obtain honey raisin tree Thick many candies, then will be added in after honey raisin tree Thick many candies rehydration
Chloroform and n-butanol removing protein, then water intaking mutually add in chloroform and n-butanol again, are obtained after taking off albumen three times by freeze-drying
Obtain hovenia polysaccharides.
Probiotics are added to by the mass ratio of 0.05%-0.2% in pig, ox, sheep, chicken animal and fowl fodder and mixed by the present invention
Uniformly probiotics feed is made in conjunction.
Lactobacillus acidophilus and Paula enlightening yeast can improve livestock and poultry intestinal microflora equilibrium and pre- in probiotics of the present invention
Anti- to have loose bowels, the addition of synanthrin and galactooligosaccharide promotes livestock and poultry to the absorption of nutriment and promotes proliferation of intestinal probiotics, plants
The addition of sour enzyme increases utilization rate of the livestock and poultry to phytic acid in feed, reduces the utilization rate of microcosmic salt and the discharge of phosphorus, the present invention
The probiotics disease resistance that increases livestock and poultry and the Utilization ability to feed, the speed of growth it is fast.
Specific embodiment
With reference to specific embodiment, the present invention is described in further detail.
Embodiment 1：
The probiotics of the present embodiment use single lactobacillus acidophilus bacterium powder, and viable count is 2.1 × 1011CFU/g-
6.0×1011CFU/g。
It is prepared by lactobacillus acidophilus bacterium powder：
The glucose of addition puncture vine extracting solution quality 1%, 0.2% casein hydrolysate, 0.1% in puncture vine extracting solution
Sodium acetate and 0.25% diammonium hydrogen citrate, then by 2% volume ratio access lactobacillus acidophilus, in 37 DEG C of constant temperature incubations
After 18h, in 4 DEG C, 6000rpm centrifugations 20min obtains lactobacillus acidophilus bacterium mud, then adds the compound freeze-drying of 3 times of bacterium mud weight
It is for 24 hours 3.0 × 10 to get viable count that protective agent, which is placed on vacuum freeze drier drying,11CFU/g-6.0×1011CFU/g's
Lactobacillus acidophilus bacterium powder；
Every the 100g compound freeze drying protectant 5g containing phytase, galactooligosaccharide 15g, hovenia polysaccharides 5g, soya-bean oil 3g, rely
Propylhomoserin 3g, choline chloride 3g, phosphate buffer 25g, distilled water 41g.
Probiotics are added to by 0.05% mass ratio in pig, ox, sheep, chicken animal and fowl fodder and are uniformly mixed by the present invention
Probiotics feed is made.
Embodiment 2：
The probiotics of the present embodiment use single Paula enlightening yeast bacterium powder, and viable count is 3.0 × 1010CFU/g-
5.0×1010CFU/g。
It is prepared by Paula enlightening yeast bacterium powder：
Fresh honey raisin tree is taken to be beaten to obtain honey raisin tree slurry after cleaning, the pectin of honey raisin tree slurry quality 1% is separately added into honey raisin tree slurry
Enzyme, cellulase and hemicellulase keep the temperature 2h in 60 DEG C, then filter and obtain hovenia acerba juice, are connect by volume after being cooled to 38 DEG C
Enter the 1% Paula enlightening yeast activated, after 37 DEG C of constant temperature incubation 28h, at 4 DEG C, 4000rpm centrifugations 20min collects Paula
Enlightening yeast bacterium mud, then the compound freeze drying protectant of addition 2 times of weight of bacterium mud, which is placed in vacuum freeze drier, is freeze-dried
It is for 24 hours 3.0 × 10 to get viable count10CFU/g-5.0×1010The Paula enlightening saccharomycete bacterium powder of CFU/g；
The 100g compound freeze drying protectant 10g containing synanthrin, trehalose 8g, threonine 0.4g, methionine 2g, sodium chloride
2g, phosphate buffer 20g, distilled water 57.6g.
Probiotics are added to by 0.1% mass ratio in pig, ox, sheep, chicken animal and fowl fodder and are uniformly mixed by the present invention
Probiotics feed is made.
Embodiment 3：
The probiotics of the present embodiment are compound micro-ecological preparation by Paula enlightening yeast bacterium powder and lactobacillus acidophilus bacterium powder
By 1:1 mass ratio composition, the wherein viable count of Paula enlightening saccharomycete bacterium powder are 1.0 × 1010CFU/g-2.5×1010CFU/g、
Lactobacillus acidophilus bacterium powder viable count is 1.0 × 1011CFU/g-3.0×1011CFU/g。
1) prepared by Paula enlightening yeast bacterium powder：
Fresh honey raisin tree is taken to be beaten to obtain honey raisin tree slurry after cleaning, the pectin of honey raisin tree slurry quality 1% is separately added into honey raisin tree slurry
Enzyme, cellulase and hemicellulase keep the temperature 2h in 60 DEG C, then filter and obtain hovenia acerba juice, are connect by volume after being cooled to 38 DEG C
Enter the 1% Paula enlightening yeast activated, after 37 DEG C of constant temperature incubation 28h, at 4 DEG C, 4000rpm centrifugations 20min collects Paula
Enlightening yeast bacterium mud, then the compound freeze drying protectant of addition 2 times of weight of bacterium mud, which is placed in vacuum freeze drier, is freeze-dried
It is for 24 hours 3.0 × 10 to get viable count10CFU/g-5.0×1010The Paula enlightening saccharomycete bacterium powder of CFU/g；
The 100g compound freeze drying protectant 10g containing synanthrin, trehalose 8g, threonine 0.4g, methionine 2g, sodium chloride
2g, phosphate buffer 20g, distilled water 57.6g；
2) prepared by lactobacillus acidophilus bacterium powder：
The glucose of addition puncture vine extracting solution quality 1%, 0.2% casein hydrolysate, 0.1% in puncture vine extracting solution
Sodium acetate and 0.25% diammonium hydrogen citrate, then by 2% volume ratio access lactobacillus acidophilus, in 37 DEG C of constant temperature incubations
After 18h, in 4 DEG C, 6000rpm centrifugations 20min obtains lactobacillus acidophilus bacterium mud, then adds the compound freeze-drying of 3 times of bacterium mud weight
It is for 24 hours 3.0 × 10 to get viable count that protective agent, which is placed on vacuum freeze drier drying,11CFU/g-6.0×1011CFU/g's
Lactobacillus acidophilus bacterium powder；
Every the 100g compound freeze drying protectant 5g containing phytase, galactooligosaccharide 15g, hovenia polysaccharides 5g, soya-bean oil 3g, rely
Propylhomoserin 3g, choline chloride 3g, phosphate buffer 25g, distilled water 41g.
Probiotics are added to by 0.15% mass ratio in pig, ox, sheep, chicken animal and fowl fodder and are uniformly mixed by the present invention
Probiotics feed is made.
Embodiment 4：
The probiotics of the present embodiment are compound micro-ecological preparation by Paula enlightening yeast bacterium powder and lactobacillus acidophilus bacterium powder
By 2:1 mass ratio composition, the wherein viable count of Paula enlightening saccharomycete bacterium powder are 1.0 × 1010CFU/g-2.5×1010CFU/g、
Lactobacillus acidophilus bacterium powder viable count is 1.0 × 1011CFU/g-3.0×1011CFU/g。
1) prepared by Paula enlightening yeast bacterium powder：
Fresh honey raisin tree is taken to be beaten to obtain honey raisin tree slurry after cleaning, the fruit of honey raisin tree slurry quality 0.5% is separately added into honey raisin tree slurry
Glue enzyme, cellulase and hemicellulase keep the temperature 3h in 60 DEG C, then filter and obtain hovenia acerba juice, are cooled to after 39 DEG C by volume
The Paula enlightening yeast activated of access 2%, after 37 DEG C of constant temperature incubation 30h, at 5 DEG C, 5000rpm centrifugations 15min, which is collected, to be protected
Lardy yeast bacterium mud, then add 2 times of weight of bacterium mud compound freeze drying protectant be placed in vacuum freeze drier freeze it is dry
Dry is for 24 hours 3.0 × 10 to get viable count10CFU/g-5.0×1010The Paula enlightening saccharomycete bacterium powder of CFU/g；
The 100g compound freeze drying protectant 12g containing synanthrin, trehalose 10g, threonine 0.6g, methionine 3g, chlorination
Sodium 2g, phosphate buffer 25g, distilled water 47.4g；
2) prepared by lactobacillus acidophilus bacterium powder：
Added in puncture vine extracting solution the glucose of puncture vine extracting solution quality 1.5%, 0.3% casein hydrolysate,
Then 0.3% sodium acetate and 0.3% diammonium hydrogen citrate access lactobacillus acidophilus, in 37 DEG C of constant temperature by 3% volume ratio
After cultivating 20h, in 6 DEG C, 7000rpm centrifugations 15min obtains lactobacillus acidophilus bacterium mud, then adds the compound of 2 times of bacterium mud weight
It is for 24 hours 3.0 × 10 to get viable count that freeze drying protectant, which is placed on vacuum freeze drier drying,11CFU/g-6.0×1011CFU/
The lactobacillus acidophilus bacterium powder of g；
Every the 100g compound freeze drying protectant 3g containing phytase, galactooligosaccharide 12g, hovenia polysaccharides 4g, soya-bean oil 2g, rely
Propylhomoserin 2g, choline chloride 5g, phosphate buffer 22g, distilled water 50g.
Probiotics are added to by 0.08% mass ratio in pig, ox, sheep, chicken animal and fowl fodder and are uniformly mixed by the present invention
Probiotics feed is made.
Embodiment 5：
The probiotics of the present embodiment are compound micro-ecological preparation by Paula enlightening yeast bacterium powder and lactobacillus acidophilus bacterium powder
By 1:2 mass ratio composition, the wherein viable count of Paula enlightening saccharomycete bacterium powder are 1.0 × 1010CFU/g-2.5×1010CFU/g、
Lactobacillus acidophilus bacterium powder viable count is 1.0 × 1011CFU/g-3.0×1011CFU/g。
1) prepared by Paula enlightening yeast bacterium powder：
Fresh honey raisin tree is taken to be beaten to obtain honey raisin tree slurry after cleaning, the fruit of honey raisin tree slurry quality 0.8% is separately added into honey raisin tree slurry
Glue enzyme, cellulase and hemicellulase keep the temperature 2.5h in 60 DEG C, then filter and obtain hovenia acerba juice, are cooled to after 40 DEG C by volume
Than the Paula enlightening yeast activated of access 5%, after 37 DEG C of constant temperature incubation 36h, at 8 DEG C, 6000rpm centrifugations 10min is collected
Paula enlightening yeast bacterium mud, then the compound freeze drying protectant of addition 2 times of weight of bacterium mud, which is placed in vacuum freeze drier, freezes
Dry is for 24 hours 3.0 × 10 to get viable count10CFU/g-5.0×1010The Paula enlightening saccharomycete bacterium powder of CFU/g；
The 100g compound freeze drying protectant 15g containing synanthrin, trehalose 12g, threonine 0.8g, methionine 5g, chlorination
Sodium 2.8g, phosphate buffer 21.5g, distilled water 42.9g；
2) prepared by lactobacillus acidophilus bacterium powder：
The glucose of addition puncture vine extracting solution quality 2%, 0.4% casein hydrolysate, 0.2% in puncture vine extracting solution
Sodium acetate and 0.4% diammonium hydrogen citrate, then by 5% volume ratio access lactobacillus acidophilus, in 37 DEG C of constant temperature incubations
After for 24 hours, in 8 DEG C, 8000rpm centrifugations 10min obtains lactobacillus acidophilus bacterium mud, then adds the compound freeze-drying of 1 times of bacterium mud weight
It is for 24 hours 2.1 × 10 to get viable count that protective agent, which is placed on vacuum freeze drier drying,11CFU/g-6.0×1011CFU/g's
Lactobacillus acidophilus bacterium powder；
Every the 100g compound freeze drying protectant 2g containing phytase, galactooligosaccharide 10g, hovenia polysaccharides 3g, soya-bean oil 1g, rely
Propylhomoserin 1g, choline chloride 3g, phosphate buffer 20g, distilled water 60g.
Probiotics are added to by 0.2% mass ratio in pig, ox, sheep, chicken animal and fowl fodder and are uniformly mixed by the present invention
Probiotics feed is made.
Claims (5)
1. a kind of probiotics, it is characterised in that：Including single lactobacillus acidophilus bacterium powder, Paula enlightening yeast bacterium powder or both
Compound micro-ecological preparation；
Described single its viable count of lactobacillus acidophilus bacterium powder is 2.1 × 1011CFU/g-6.0×1011CFU/g；
The single Paula enlightening yeast bacterium powder, viable count are 3.0 × 1010CFU/g-5.0×1010CFU/g；
The compound micro-ecological preparation presses 2 by Paula enlightening yeast bacterium powder and lactobacillus acidophilus bacterium powder:1~1:2 mass ratio group
Into wherein the viable count of Paula enlightening saccharomycete bacterium powder is 1.0 × 1010CFU/g-2.5×1010CFU/g, lactobacillus acidophilus bacterium powder
Viable count is 1.0 × 1011CFU/g-3.0×1011CFU/g。
2. a kind of preparation method of probiotics, it is characterised in that：
It is prepared by single Paula enlightening yeast bacterium powder：
Fresh honey raisin tree is taken to be beaten to obtain honey raisin tree slurry after cleaning, the pectin of honey raisin tree slurry quality 0.5-1% is separately added into honey raisin tree slurry
Enzyme, cellulase and hemicellulase keep the temperature 2-3h in 60 DEG C, then filter and obtain hovenia acerba juice, by body after being cooled to 38-40 DEG C
The Paula enlightening yeast that has activated of the product than accessing 1-5%, after 37 DEG C of constant temperature incubation 28-36h, at 4-8 DEG C, 4000-6000rpm
It centrifuges 10-20min and collects Paula enlightening yeast bacterium mud, the compound freeze drying protectant for then adding 2 times of weight of bacterium mud is placed on vacuum
Be freeze-dried in freeze drier is for 24 hours 3.0 × 10 to get viable count10CFU/g-5.0×1010The Paula enlightening yeast of CFU/g
Bacterium bacterium powder；
The 100g compound freeze drying protectant 10-15g containing synanthrin, trehalose 8-12g, threonine 0.4-0.8g, methionine 2-
5g, sodium chloride 2-2.8g, phosphate buffer 20-25g, distilled water 42.9-57.6g；
It is prepared by single lactobacillus acidophilus bacterium powder：
Added in puncture vine extracting solution the glucose of puncture vine extracting solution quality 1-2%, the casein hydrolysate of 0.2-0.4%,
Then the sodium acetate of 0.1-0.3% and the diammonium hydrogen citrate of 0.2-0.4% access lactobacillus acidophilus by the volume ratio of 2-5%,
After 37 DEG C of constant temperature incubation 18-24h, in 4-8 DEG C, 6000-8000rpm centrifugations 10-20min obtains lactobacillus acidophilus bacterium mud, so
The compound freeze drying protectant of 1-3 times of addition bacterium mud weight is placed on vacuum freeze drier and dries afterwards is for 24 hours to get viable count
2.1×1011CFU/g-6.0×1011The lactobacillus acidophilus bacterium powder of CFU/g；
Every the 100g compound freeze drying protectant 2-5g containing phytase, galactooligosaccharide 10-15g, hovenia polysaccharides 3-5g, soya-bean oil 1-
3g, lysine 1-3g, choline chloride 3-5g, phosphate buffer 20-25g, distilled water 41-60g；
It is prepared by compound micro-ecological preparation：
Single Paula enlightening yeast bacterium powder and single lactobacillus acidophilus bacterium powder are pressed 2:1~1:2 mass ratio mixing, wherein Paula enlightening
The viable count of saccharomycete bacterium powder is 1.0 × 1010CFU/g-2.5×1010CFU/g, lactobacillus acidophilus bacterium powder viable count for 1.0 ×
1011CFU/g-3.0×1011CFU/g。
3. the preparation method of probiotics according to claim 2, it is characterised in that：The preparation of the puncture vine extracting solution
Method is：Puncture vine is taken by 1：14 mass ratio soaks 25min, and then heating boils and maintains boiling 30min, then with 400
Mesh screen, adding deionized water afterwards twice with vacuum pump suction filtration after cooling makes puncture vine and water ratio be 1:10 carry up to puncture vine
Take liquid.
4. the preparation method of probiotics according to claim 2, it is characterised in that：The preparation side of the hovenia polysaccharides
Method is：The supernatant after the enlightening yeast bacterium mud of Paula will be collected by centrifugation added in after low temperature is concentrated into 1/3 the anhydrous second of 3 times of volumes
Alcohol stands 3h and filters to obtain honey raisin tree Thick many candies, then chloroform and n-butanol removing protein will be added in after honey raisin tree Thick many candies rehydration, then fetches water
Chloroform and n-butanol are mutually added in again, and hovenia polysaccharides are obtained by freeze-drying after de- albumen three times.
5. a kind of application of probiotics as described in claim 1, it is characterised in that：Probiotics are pressed into 0.05%-
0.2% mass ratio, which is added to be uniformly mixed in pig, ox, sheep, chicken animal and fowl fodder, is made probiotics feed.
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