CN1406589A - Insect health-care products for liver and stomach and preparation thereof - Google Patents
Insect health-care products for liver and stomach and preparation thereof Download PDFInfo
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- CN1406589A CN1406589A CN01142086A CN01142086A CN1406589A CN 1406589 A CN1406589 A CN 1406589A CN 01142086 A CN01142086 A CN 01142086A CN 01142086 A CN01142086 A CN 01142086A CN 1406589 A CN1406589 A CN 1406589A
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- fly larvae
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Abstract
A health-care food for protecting liver, nourishing stomach, and improving immunity is prepared from the artificially cultured fly maggot through dual physical inducing and proportionally adding the extract of fly maggot shell. Its advantage is rich nutrients (insection protein, amino acids, etc) and medicinal components (antibacterial peptide, chitosan, etc).
Description
Technical field
The invention belongs to medicine and field of health care food, relate to a kind of insecticide health food and preparation method thereof, relate in particular to a kind of induced product that utilizes Musca domestica larva (fly larvae) and extraction of substance and be health product that active ingredient gets and preparation method thereof with liver-protecting and stomach-nourishing function.
Background technology
Fly larvae is as medicinal, (worm order the 40 volume) promptly on the books in the Compendium of Material Medica of Li Shizhen (1518-1593 A.D.): " maggot claims ' Chrysomyiame gacephala (Fab.) ', ' Luo Xianzi ', ' Semen Narcissi Chinensis ' again, can control children's Gan Re, for controlling the good medicine of infantile malnutrition ".In recent years, the development and use to fly larvae both at home and abroad have more report, mainly concentrate on following three aspects:
1, the exploitation fly larvae substitutes fish flour or preparation feed additive as feedstuff, addresses as 1122197,1212131,1256880 of patent disclosures, though feedstuff series products market is bigger, the added value of utilizing is lower, has not more given play to the pharmaceutical potential of fly larvae.
2, utilize fly larvae to produce health food or medicine, once utilized the fly larvae Development and Production for human edible nutritional activities dry powder, for the development and use of fly larvae resource provide a high-level application approach as patent disclosure 1141130,1231131,1260145.
3, utilize fly larvae to extract useful component, as hydrolyzed protein, chitin etc.All reported as patent disclosure 1243836,1250057,1266855 and to have utilized the fly larvae chitin extraction.
More than various reports all adopt the fly larvae of inducing processing without any, thereby the immunologic mechanism of fly larvae is difficult to transfer, the intravital composition of worm is difficult to reach comprehensively, effective; Moreover, more than all do not relate in the report and utilize fly larvae to produce liver-protecting and stomach-nourishing function health food or medicine; In addition, more than all do not relate in the report with after the centrifugal insect shell that goes out is prepared into chitosan, utilize again with behind the fly larvae activated protein uniform mixing.Therefore above various reports had not both given full play of the medicinal advantage of fly larvae, had not given play to the medicinal advantage of chitosan yet.
Summary of the invention
The objective of the invention is to overcome the defective of in the prior art fly larvae directly being utilized without inducing, overcome the defective of utilizing fly larvae merely in the prior art and not adding maggot shell extract-chitosan, the brand-new health food that a kind of nutritional labeling height is provided, medicinal ingredient is many and has hepatoprotective, nourishing the stomach effect.
Another object of the present invention is to overcome the defective workmanship of in the prior art fly larvae directly being processed without inducing, overcome the defective of not utilizing maggot shell in the prior art and wasting resource, a kind of dual physics inducement fly larvae is provided, and utilizes maggot shell to prepare the production technology of chitosan.
The object of the present invention is achieved like this:
Health food provided by the invention is solid-state shape granule or powder, and its component by weight percentage, comprising:
Chitosan 5～15%;
Insect albumen powder 85～95%.
Health food color and luster of the present invention is yellowish, and little band seafood flavor, the visible matter of no naked eyes, and nothing is gone mouldy.Health food of the present invention can be made various dosage forms on demand, as making capsule or tablet.
Health food of the present invention, its effect come from induce and insect active albumen and utilize maggot shell and chitosan.Wherein contain the needed several amino acids of animal in the insect active albumen, its TEAA is 43.83%, surpass the reference value 40% that Food and Agriculture Organization/World Health Organization (WHO) (FAO/WHO) proposes, its TEAA/non essential amino acid total amount value (E/N) is 0.78, surpasses the reference value 0.6 that FAO/WHO proposes.In addition, also contain in the insect active albumen through inducing technology and compositions such as a large amount of " antibacterial substance ", lysozyme that produces, agglutinin.Thereby insect active albumen has immunomodulating, resisting fatigue, radioprotective, the liver protecting, the anti-ageing health-care effect of waiting for a long time.
In addition, the chitosan that utilizes maggot shell to extract and prepare be the unique existence of occurring in nature cation edibility fiber, it has blood fat reducing, blood pressure lowering, blood sugar lowering, promotion cell division and growth, strengthens the enteral probiotics, suppresses harmful bacterium, reinforced immunological function, slow down aging, inhibition tumor cell, strengthen physiological effecies such as liver function, infection.
The preparation method of health food provided by the invention may further comprise the steps:
1, thermal induction is handled.When treating that fly larvae grows to 3 ages in days, take out culture plate, handle about 30min with 50 ℃ of hot blasts.
2, the worm material separates.With the light separation facilities the inductive fly larvae of institute is separated with feedstuff.
3, stab and induce processing.Handle isolating fly larvae 15～30min with homemade pin type electric vibrating sieve, stab the purpose that polypide and clean polypide adhere to feedstuff to reach.
4, follow-up cultivation.The polypide of stabbing after the processing is cultivated 24～48Hr under the environmental condition of 25 ± 2 ℃ of temperature, humidity 60～70%, stops to cultivate during larvae pupation until about 30%, during only supply suitable water and stop for food.
5, broken, homogenate.Fly larvae after will stopping to cultivate or just pupa with colloid mill grind, homogenate;
6, centrifugal treating.Collect the fly larvae serosity after grinding, with high speed centrifuge filtering residue is separated with filtrate, filtrate enters the 7th step process, and filtering residue enters the 9th step process.
7, lyophilization.With vacuum freeze drier filtrate is dried.
8, collect, pulverize.The dry powder of collection after lyophilization is pulverized.Be the activity of the insect albumen powder that adequately protects, must dry processing with freezer dryer.
9, chitin extracts.Filtering residue (maggot shell) is poured in the stirring reaction pot, 3% hydrochloric acid that adds 2～3 times, slough the calcium in the shell, 10% sodium hydroxide solution that adds 2～3 times, remove the albumen in shelling, in stirring clay kettle, add 2～3% potassium permanganate solutions at last, soak bleaching 1 hour, promptly get white solid product chitin.
10, Preparation of Chitosan.The chitin finished product is immersed in 50% sodium hydroxide solution, is warming up to 100 ℃, stirring reaction 1 hour, filtering alkali liquor is washed to pH value for neutral.Vacuum drying promptly gets white or slightly has the translucent chitosan of pearly luster.
11, allotment, packing.With dry powder and prepared chitosan in 85～95: 5～15 ratio is allocated, is packed.
According to the above mode of production, can produce and be rich in antimicrobial active protein, agglutinin, lysozyme, chitosan and protein, aminoacid, and have the insect health-care products of the effect of hepatoprotective, nourishing the stomach.
In each step of dry powder preparation of the present invention, temperature all need remain on below 80 ℃.Because when temperature is higher than 80 ℃, the effective ingredient in the fly larvae polypide has ruined possibility.In addition, because " high rhythm " characteristics of maggot grow, thereby between above each production link, must closely connect, to avoid rotten or browning phenomenon occurs.Production between the every batch of product also must closely connect.Process chart as shown in Figure 1 is preparation technology's flow chart of health food of the present invention.
The preparation method of health food provided by the invention, at first fly larvae is carried out dual physics inducement, to roll up the generation of medical substances such as antibiotic substance, agglutinin and lysozyme in the fly larvae body, adopt the production technology of system then, effective ingredient in the fly larvae (as antibacterial peptide, agglutinin, lysozyme etc.) and nutritional labeling (as protein, aminoacid, lipid etc.) are extracted to greatest extent, make the insect active egg albumen powder.Moreover, the chitin in the maggot shell is extracted, and be prepared into chitosan.At last, with active protein powder and the formulated according to a certain percentage health food of the present invention of chitosan.So just give full play of the function of fly larvae active protein powder and chitosan, the effect of reach hepatoprotective, nourishing the stomach, keeping fit.In a sense, this product is a fly larvae " an activation body ", has both induced activated protein, agglutinin and lysozyme, extracts chitosan again, and effect should be better than the instrument sold on the current market product with the drying one-tenth powder of fly larvae.
Compared with the prior art the present invention has following advantage and characteristics:
1, can obviously improve the immunocompetence of human body by the prepared health product of the present invention, reach the effect of hepatoprotective, nourishing the stomach.
2, the fly larvae after the present invention will induce first and the extract-chitosan applied in any combination of shell thereof are in the medicines and health protection field.
3, technological process elder generation and then actual effect involved in the present invention makes the Musca domestica larva resource obtain comprehensive utilization preferably.
The specific embodiment
Embodiment 1
The preparation of insecticide health food
5 dishes are grown to the fly larvae culture plate in 3 ages shift out feeding room, inducing the indoor 50 ℃ of hot blasts processing of processing 30min, with seperator the inductive fly larvae of institute is separated with feedstuff then, the isolating 5kg fly larvae of institute is placed on the homemade pin type vibrosieve behind the vibration processing 30min, cultivate 36Hr 70% time in 25 ℃ of temperature, humidity, stop during larvae pupation until about 30% cultivating, then with colloid mill with fly larvae or pupa fragmentation just, homogenate.Collect the fly larvae serosity, centrifugal, get filtrate, with freezer dryer filtrate is dried, collect dry powder, sterilization gets the insect active egg albumen powder; Get filtering residue, the preparation chitosan mixes dry powder in 90: 10 ratio then with chitosan, mix well, and packs, and promptly obtains the insecticide health food of 1.25kg tool hepatoprotective, nourishing the stomach.
Embodiment 2
20 dishes are grown to the fly larvae culture plate in 3 ages shift out feeding room, inducing the indoor 50 ℃ of hot blasts processing of processing 30min, with seperator the inductive fly larvae of institute is separated with feedstuff then, the isolating 20kg fly larvae of institute is placed on the homemade pin type vibrosieve behind the vibration processing 20min, cultivate 24Hr 70% time in 25 ℃ of temperature, humidity, stop during larvae pupation until about 30% cultivating, then with colloid mill with fly larvae or pupa fragmentation just, homogenate.Collect the fly larvae serosity, centrifugal, get filtrate, with freezer dryer filtrate is dried, collect dry powder, sterilization gets the insect active egg albumen powder; Get filtering residue, the preparation chitosan mixes dry powder in 85: 15 ratio then with chitosan, mix well, and packs, and promptly obtains the insecticide health food of 9.5kg tool hepatoprotective, nourishing the stomach.
Embodiment 3
The liver protection effect of insecticide health food
60 white mice are divided into 5 groups at random, 12 every group, are respectively normal control group (I group), CCl4 damage group (II group), invention product protection group (III group, IV group, V group).The I group is not done special handling; II group carbon tetrachloride (0.1%CCl
4, 10ml/kg.b.w) to handle, III group, IV group, V group all add carbon tetrachloride (0.1%CCl
4, 10ml/kg.b.w) handle, and add drink 5% invention product suspension 1.25,2.50,5.0ml/kg.b.w respectively.4 of every group of stochastic sampling mices, in CCl4 handle the blood sampling of the capable mice socket of the eye of back 48h venous sinus, separation of serum is measured its sudismase (SOD) and sweet peptide peroxidase (GSH-Px) vigor of Guang and malonaldehyde (MDA) content; Put to death then and cut open the belly and get hepatic tissue and measure its SOD, GSH-Px, MDA content (table 1, table 2).The result shows, uses product of the present invention and can significantly improve hepatic tissue and SOD in serum and GSH-Px vigor, reduces MDA content, to a certain degree free radical resisting and lipoid peroxidization resistant is arranged, to CCl
4Due to hepatic injury comparatively sure protective effect is arranged.
Table 1. CCl4 handles back 48h hepatic tissue SOD, GSH-Px and MDA content grouping SOD (ug/g) GSH-PX (ug/g) MDA (nmol/g) I group 349.189 ± 45.250 163.324 ± 12.314 23.495 ± 6.059 II group 93.499 ± 28.112 102.125 ± 11.036 41.333 ± 5.937 III group 166.886 ± 32.806* △ 186.246 ± 13.269* △ 28.546 ± 6.034* △ IV group 174.374 ± 30.645* △ 193.439 ± 14.001* △ 29.332 ± 5.865* △ V group 175.721 ± 29.462* △ 192.835 ± 13.132* △ 30.102 ± 5.914* △ annotate: * compares with the I group, P＜0.05, △ compares P＜0.01 with the II group
Table 2. SOD in serum content
Grouping GSH-Px content (μ g/ml) MDA (nmol) SOD content (nu/ml)
I group 81.346 ± 2.916 5.129 ± 1.291 38.913 ± 3.125
II group 68.149 ± 3.699 11.936 ± 3.435 28.822 ± 2.011
III organizes 89.936 ± 3.117** 5.021 ± 2.036 46.842 ± 3.115**
IV organizes 88.961 ± 3.660** 4.834 ± 0.984 45.946 ± 3.612**
V group 90.165 ± 3.126** 4.308 ± 1.103 46.734 ± 3.121** annotate: compare * * P＜0.01 with the II group
Embodiment 4
The stomach-nourishing function of insecticide health food
To fasting rat oral gavage product of the present invention, irritate stomach dehydrated alcohol 1.0ml/ Mus after 45 minutes, put to death animal after 1.0 hours, to cut off the abdominal cavity and take out stomach, gastric injects 10% formaldehyde and is fixed in 10% formaldehyde.Open stomach and calculate the stomach mucosa damaged area.Experimental result shows: product of the present invention promptly has good protective effect to this model under very low dose of, promptly reaches the effect (table 3) of SANJIU WEITAI 5.0g/Kg with the effect of 2.0g/Kg.Cimetidine does not demonstrate protective effect to this model under all dosage.
Table 3. invention product is to the effect of rat no-alcohol type gastritis model
The sticking damage number suppression ratio of touching of group dosage (g/kg) number of animals
Contrast-8 35.1 ± 33.8
Invention product 0.50 8 6.6 ± 5.1 20.7
Invention product 2.00 8 1.4 ± 2.1 95.9
Invention product 5.00 8 0.5 ± 1.0 99.2*
SANJIU WEITAI 5.00 8 1.1 ± 2.0 97.1*
Cimetidine 0.10 8 60.9 ± 43.1*: compare P＜0.05 with matched group
Claims (4)
1, a kind of insect health-care products with liver-protecting and stomach-nourishing function, it is characterized in that it be by artificial Musca domestica larva of culturing (fly larvae) through certain physics inducement, and add part maggot shell extract in proportion and the pure natural, the Chinese medicine class insect health-care products that prepare.
2, according to the said insecticide health food of claim 1, it is solid-state shape granule or powder, it is characterized in that, this insecticide health food component by weight percentage, comprising: 5～15% chitosan; 85～95% insect albumen powder.
3, a kind of preparation method as claim 1,2 any described insecticide health foods is characterized in that, may further comprise the steps:
(1), thermal induction is handled.When treating that fly larvae grows to 3 ages in days, take out culture plate, handle 30min with 50 ℃ of hot blasts;
(2), the worm material separates.With the light separation facilities inducing fly larvae is separated with feedstuff;
(3), stab and induce processing.Handle isolating fly larvae 15～30min with homemade pin type electric vibrating sieve, stab the purpose that polypide and clean polypide adhere to feedstuff to reach;
(4), follow-up cultivation.The polypide of stabbing after the processing is cultivated 24～48Hr under the environmental condition of 25 ± 2 ℃ of temperature, humidity 60～70%, stops to cultivate during larvae pupation until about 30%, during only supply suitable water and stop for food;
(5), broken, homogenate.Fly larvae after will stopping to cultivate or just pupa with colloid mill grind, homogenate;
(6), centrifugal treating.Collect the fly larvae serosity after grinding, with high speed centrifuge filtering residue is separated with filtrate, filtrate prepares dry powder, and filtering residue extracts chitin;
(7), lyophilization.With vacuum freeze drier filtrate is dried;
(8), collect, pulverize.The dry powder of collection after lyophilization is pulverized.
(9), chitin extracts.Filtering residue (maggot shell) through decalcification, except that processes such as albumen, bleachings, is got white solid product chitin.
(10), Preparation of Chitosan.With the chitin finished product through processes such as digestion, dryings, white or slightly have pearly luster, a translucent chitosan.
(11), allotment, packing.With dry powder and prepared chitosan in 85～95: 5～15 ratio is allocated, is packed, both insect health-care products of the present invention.
4, according to claim 1,2 said insecticide health foods, it is characterized in that: described chitosan also can directly be bought from market and allocate with the insect albumen powder that the present invention makes.
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| CN01142086A CN1406589A (en) | 2001-09-11 | 2001-09-11 | Insect health-care products for liver and stomach and preparation thereof |
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| CN01142086A CN1406589A (en) | 2001-09-11 | 2001-09-11 | Insect health-care products for liver and stomach and preparation thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1293886C (en) * | 2004-12-09 | 2007-01-10 | 何明华 | Medication possessign curative effect of anti hepatocinhosis for treating liver disease and preparation method |
| CN100339020C (en) * | 2004-10-25 | 2007-09-26 | 贵州益佰制药股份公司 | Balance food and its preparing process |
| CN101406486B (en) * | 2008-11-05 | 2011-06-08 | 梁庆高 | Method for preparing maggot health-care oral liquid |
| CN102273430A (en) * | 2010-06-09 | 2011-12-14 | 张春燕 | Culturing method of maggot containing toad toxication component and treating function of preparation of maggot |
| WO2014017451A1 (en) * | 2012-07-27 | 2014-01-30 | 国立大学法人愛媛大学 | Polysaccharide, composition containing polysaccharide, and immunostimulating agent |
| CN107510044A (en) * | 2017-09-09 | 2017-12-26 | 李绍波 | A kind of clinical liver disease nutrient formulation and preparation method thereof |
-
2001
- 2001-09-11 CN CN01142086A patent/CN1406589A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100339020C (en) * | 2004-10-25 | 2007-09-26 | 贵州益佰制药股份公司 | Balance food and its preparing process |
| CN1293886C (en) * | 2004-12-09 | 2007-01-10 | 何明华 | Medication possessign curative effect of anti hepatocinhosis for treating liver disease and preparation method |
| CN101406486B (en) * | 2008-11-05 | 2011-06-08 | 梁庆高 | Method for preparing maggot health-care oral liquid |
| CN102273430A (en) * | 2010-06-09 | 2011-12-14 | 张春燕 | Culturing method of maggot containing toad toxication component and treating function of preparation of maggot |
| WO2014017451A1 (en) * | 2012-07-27 | 2014-01-30 | 国立大学法人愛媛大学 | Polysaccharide, composition containing polysaccharide, and immunostimulating agent |
| CN104884068A (en) * | 2012-07-27 | 2015-09-02 | 株式会社爱南自由 | Polysaccharide, composition containing polysaccharide, and immunostimulating agent |
| CN107510044A (en) * | 2017-09-09 | 2017-12-26 | 李绍波 | A kind of clinical liver disease nutrient formulation and preparation method thereof |
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