CN108013261A - One breeder probiotics feed and preparation method thereof - Google Patents

One breeder probiotics feed and preparation method thereof Download PDF

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Publication number
CN108013261A
CN108013261A CN201711487585.4A CN201711487585A CN108013261A CN 108013261 A CN108013261 A CN 108013261A CN 201711487585 A CN201711487585 A CN 201711487585A CN 108013261 A CN108013261 A CN 108013261A
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chicken
preparation
feed
cfu
lactobacillus acidophilus
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黄丹
翟亚玲
张文晔
乔冰涛
赵振国
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Shaanxi Yangling Shiyang Feed Co Ltd
Shaanxi Shiyang Agricultural Polytron Technologies Inc
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Shaanxi Yangling Shiyang Feed Co Ltd
Shaanxi Shiyang Agricultural Polytron Technologies Inc
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Priority to CN201711487585.4A priority Critical patent/CN108013261A/en
Publication of CN108013261A publication Critical patent/CN108013261A/en
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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  • Polymers & Plastics (AREA)
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  • Biomedical Technology (AREA)
  • Fodder In General (AREA)

Abstract

One breeder probiotics feed and preparation method thereof, wherein containing lactobacillus acidophilus and Paula enlightening yeast to improve the production performance of chicken and the general level of the health, improve efficiency of feed utilization.Contained per 1000g chickens with probiotics feed：544 721g of corn, 60 100g of dregs of beans, 80 120g of wheat bran, 10 30g of cotton dregs, 10 30g of glutamic acid slag, 80 100g of vinasse, 10 20g of whitewashing maize peel, 10 20g of bluestone powder, 5 10g of medical stone, 1 3g of soya-bean oil, 2 5g of compound micro-ecological preparation, 70% lysine, 1 2g, 5 7g of calcium bicarbonate, 1 2g of methionine, 3 5g of composite mineral matter, 1 3g of multi-vitamins.Chicken of the present invention can improve chicken intestinal microflora equilibrium with lactobacillus acidophilus in probiotics feed and Paula enlightening yeast and prevention chicken has loose bowels, the addition of synanthrin and galactooligosaccharide promotes absorption and promotion proliferation of intestinal probiotics of the chicken to nutriment, the addition of phytase adds utilization rate of the chicken to phytic acid in feed, reduce the utilization rate of microcosmic salt and the discharge of phosphorus, the chicken of the present invention adds the disease resistance of chicken and the Utilization ability to feed with probiotics, and the speed of growth is fast.

Description

One breeder probiotics feed and preparation method thereof
Technical field
The present invention relates to breeding hen feed and preparation method thereof, a more particularly to breeder probiotics feed and its system Preparation Method.
Background technology
Antibiotic animal husbandry field application, to promote animal husbandry scale development and modernization cultivation play it is huge Impetus, however, due to the abuse of antibiotic, generates a variety of side effects, and particularly antibiotic is residual in animal products Stay, directly threaten the health of the mankind, cause the potential hazard of livestock products.From in January, 2006 so far, European Union completely forbids anti- Raw element is used for animal feed.China have every year export to Japan, the U.S., the animal products of European Union are given with a variety of technical barriers To retract or destroy with limitation, its exceeded drug residue is the main problem for influencing China's animal products quality, and I State's livestock products enter the major obstacle of international market.In view of the foregoing, to avoid abuse of antibiotics medicine from causing medicine residual The potential hazard stayed, exploitation have effects that the probiotics of prevention disease and feed are very urgent.Since probiotics has Functional more, the advantages that having no toxic side effect, do not remain, is free from environmental pollution, it has also become ideal indirect substitute antibiotics medicine Thing and there is one of product of diseases prevention.
Formed according to strain, probiotics can be divided into single bacteria preparation and composite bacteria preparation, the preparation of composite bacteria preparation The preparation of single bacteria preparation is much difficult to, presently disclosed compound bacteria probiotics is few, therefore develops the micro- life of compound bacteria State preparation and feed are necessary.
The content of the invention
It is an object of the invention to provide a kind of chicken probiotics feed containing lactobacillus acidophilus and Paula enlightening yeast And preparation method thereof, to improve the production performance of chicken and the general level of the health, improve efficiency of feed utilization.
In order to achieve the above object, chicken of the present invention is contained with probiotics feed per 1000g chickens probiotics feed： Corn 544-721g, dregs of beans 60-100g, wheat bran 80-120g, cotton dregs 10-30g, glutamic acid slag 10-30g, vinasse 80-100g, spray Maize peel 10-20g, bluestone powder 10-20g, medical stone 5-10g, soya-bean oil 1-3g, compound micro-ecological preparation 2-5g are starched, 70% relies ammonia Sour 1-2g, calcium bicarbonate 5-7g, methionine 1-2g, composite mineral matter 3-5g, multi-vitamins 1-3g.
The compound micro-ecological preparation presses 2 by Paula enlightening saccharomycete bacterium powder and lactobacillus acidophilus bacterium powder:1~1:2 quality Than composition, the wherein viable count of Paula enlightening saccharomycete bacterium powder is 1.0 × 1010CFU/g-2.5×1010CFU/g, lactobacillus acidophilus The viable count of bacterium powder is 1.0 × 1011CFU/g-3.0×1011CFU/g。
The preparation method of chicken of the present invention probiotics feed,
1) prepared by Paula enlightening yeast bacterium powder：
Take fresh honey raisin tree to be beaten to obtain honey raisin tree slurry after cleaning, be separately added into honey raisin tree slurry quality 0.5-1%'s in being starched to honey raisin tree Pectase, cellulase and hemicellulase keep the temperature 2-3h in 60 DEG C, then filter and obtain hovenia acerba juice, after being cooled to 38-40 DEG C The Paula enlightening yeast activated of 1-5% is accessed by volume, after 37 DEG C of constant temperature incubation 28-36h, at 4-8 DEG C, 4000- 6000rpm centrifugations 10-20min collects Paula enlightening yeast bacterium mud, then adds the compound freeze drying protectant postposition of 2 times of weight of bacterium mud 24h is freeze-dried in vacuum freeze drier, is 3.0 × 10 up to viable count10CFU/g-5.0×1010The Paula of CFU/g Enlightening saccharomycete bacterium powder；
The 100g compound freeze drying protectant 10-15g containing synanthrin, trehalose 8-12g, threonine 0.4-0.8g, egg ammonia Sour 2-5g, sodium chloride 2-2.8g, phosphate buffer 20-25g, distilled water 42.9-57.6g；
2) prepared by lactobacillus acidophilus bacterium powder：
Glucose, the casein hydrolysis of 0.2-0.4% of puncture vine extracting solution quality 1-2% are added in puncture vine extracting solution The diammonium hydrogen citrate of thing, the sodium acetate of 0.1-0.3% and 0.2-0.4%, then accesses acidophilus breast bar by the volume ratio of 2-5% Bacterium, after 37 DEG C of constant temperature incubation 18-24h, in 4-8 DEG C, 6000-8000rpm centrifugations 10-20min obtains lactobacillus acidophilus bacterium mud, Then the compound freeze drying protectant for adding 1-3 times of bacterium mud weight is placed on vacuum freeze drier drying 24h, is up to viable count 2.1×1011CFU/g-6.0×1011The lactobacillus acidophilus bacterium powder of CFU/g；
Every 100g compound freeze drying protectant 2-5g containing phytase, the galactooligosaccharide 10-15g, hovenia polysaccharides 3-5g, beans Oily 1-3g, lysine 1-3g, choline chloride 3-5g, phosphate buffer 20-25g, distilled water 41-60g；
3) Paula enlightening saccharomycete bacterium powder and lactobacillus acidophilus bacterium powder are pressed 2:1~1:2 mass ratio is mixed compound micro- Ecological agent；
4) corn 544-721g, dregs of beans 60-100g, wheat bran 80-120g, cotton dregs 10-30g, glutamic acid are added in every 1000g It is slag 10-30g, vinasse 80-100g, whitewashing maize peel 10-20g, bluestone powder 10-20g, medical stone 5-10g, soya-bean oil 1-3g, compound Probiotics 2-5g, 70% lysine 1-2g, calcium bicarbonate 5-7g, methionine 1-2g, composite mineral matter 3-5g, compound dimension life Plain 1-3g obtains chicken probiotics feed.
The preparation method of the puncture vine extracting solution is：Puncture vine is taken by 1：14 mass ratio soaks 25min, then heats Boil and maintain boiling 30min, then with 400 mesh screens, filtered after cooling with vacuum pump and add deionized water afterwards twice and make Puncture vine and water ratio are 1:10 up to puncture vine extracting solution.
The preparation method of the hovenia polysaccharides is：The supernatant being collected by centrifugation after the enlightening yeast bacterium mud of Paula of step 1) is passed through Low temperature is concentrated into the absolute ethyl alcohol that 3 times of volumes are added after 1/3, stands 3h and filters to obtain honey raisin tree Thick many candies, then honey raisin tree Thick many candies are answered Chloroform and n-butanol removing protein are added after water, then water intaking mutually adds chloroform and n-butanol again, three times by cold after de- albumen Freeze dry acquisition hovenia polysaccharides.
Chicken of the present invention can improve chicken intestinal flora with lactobacillus acidophilus in probiotics feed and Paula enlightening yeast and put down Weighing apparatus and prevention chicken have loose bowels, and the addition of synanthrin and galactooligosaccharide promotes absorption and promotion beneficial bacteria of intestinal tract of the chicken to nutriment to increase Grow, the addition of phytase adds utilization rate of the chicken to phytic acid in feed, reduces the utilization rate of microcosmic salt and the discharge of phosphorus, this hair Bright chicken adds the disease resistance of chicken and the Utilization ability to feed with probiotics, and the speed of growth is fast.
Embodiment
With reference to specific embodiment, the present invention is described in further detail.
Embodiment 1：
Chicken is contained with probiotics feed per 1000g chickens probiotics feed：Corn 544g, dregs of beans 100g, wheat bran It is 120g, cotton dregs 30g, glutamic acid slag 30g, vinasse 100g, whitewashing maize peel 20g, bluestone powder 20g, medical stone 10g, soya-bean oil 3g, multiple Close probiotics 5g, 70% lysine 2g, calcium bicarbonate 6g, methionine 2g, composite mineral matter 5g, multi-vitamins 3g.
The compound micro-ecological preparation presses 1 by Paula enlightening saccharomycete bacterium powder and lactobacillus acidophilus bacterium powder：1 mass ratio group Into wherein the viable count of Paula enlightening saccharomycete bacterium powder is 1.0 × 1010CFU/g-2.5×1010CFU/g, lactobacillus acidophilus bacterium powder Viable count be 1.0 × 1011CFU/g-3.0×1011CFU/g。
The preparation method of chicken of the present invention probiotics feed,
1) prepared by Paula enlightening yeast bacterium powder：
Take fresh honey raisin tree to be beaten to obtain honey raisin tree slurry after cleaning, the pectin of honey raisin tree slurry quality 1% is separately added into being starched to honey raisin tree Enzyme, cellulase and hemicellulase keep the temperature 2h in 60 DEG C, then filter and obtain hovenia acerba juice, are connect by volume after being cooled to 38 DEG C Enter the 1% Paula enlightening yeast activated, after 37 DEG C of constant temperature incubation 28h, at 4 DEG C, 4000rpm centrifugations 20min collects Paula Enlightening yeast bacterium mud, then the compound freeze drying protectant of addition 2 times of weight of bacterium mud, which is placed in vacuum freeze drier, is freeze-dried 24h, is 3.0 × 10 up to viable count10CFU/g-5.0×1010The Paula enlightening saccharomycete bacterium powder of CFU/g；
The 100g compound freeze drying protectant 10g containing synanthrin, trehalose 8g, threonine 0.4g, methionine 2g, sodium chloride 2g, phosphate buffer 20g, distilled water 57.6g；
2) prepared by lactobacillus acidophilus bacterium powder：
The glucose of addition puncture vine extracting solution quality 1%, 0.2% casein hydrolysate, 0.1% in puncture vine extracting solution Sodium acetate and 0.25% diammonium hydrogen citrate, then by 2% volume ratio access lactobacillus acidophilus, in 37 DEG C of constant temperature incubations After 18h, in 4 DEG C, 6000rpm centrifugations 20min obtains lactobacillus acidophilus bacterium mud, then adds the compound lyophilized of 3 times of bacterium mud weight Protective agent is placed on vacuum freeze drier drying 24h, is 2.1 × 10 up to viable count11CFU/g-6.0×1011CFU/g's Lactobacillus acidophilus bacterium powder；
Every the 100g compound freeze drying protectant 5g containing phytase, galactooligosaccharide 15g, hovenia polysaccharides 5g, soya-bean oil 3g, rely Propylhomoserin 3g, choline chloride 3g, phosphate buffer 25g, distilled water 41g；
3) Paula enlightening saccharomycete bacterium powder and lactobacillus acidophilus bacterium powder are pressed 1:1 mass ratio is mixed and made into compound microecological system Agent；
4) corn 544g, dregs of beans 100g, wheat bran 120g, cotton dregs 30g, glutamic acid slag 30g, vinasse are added in every 1000g 100g, whitewashing maize peel 20g, bluestone powder 20g, medical stone 10g, soya-bean oil 3g, compound micro-ecological preparation 5g, 70% lysine 2g, Calcium bicarbonate 6g, methionine 2g, composite mineral matter 5g, multi-vitamins 3g.
Obtain chicken probiotics feed.
The preparation method of the puncture vine extracting solution is：Puncture vine is taken by 1：14 mass ratio soaks 25min, then heats Boil and maintain boiling 30min, then with 400 mesh screens, filtered after cooling with vacuum pump and add deionized water afterwards twice and make Puncture vine and water ratio are 1:10 up to puncture vine extracting solution.
The preparation method of the hovenia polysaccharides is：The supernatant being collected by centrifugation after the enlightening yeast bacterium mud of Paula of step 1) is passed through Low temperature is concentrated into the absolute ethyl alcohol that 3 times of volumes are added after 1/3, stands 3h and filters to obtain honey raisin tree Thick many candies, then honey raisin tree Thick many candies are answered Chloroform and n-butanol removing protein are added after water, then water intaking mutually adds chloroform and n-butanol again, three times by cold after de- albumen Freeze dry acquisition hovenia polysaccharides.
Embodiment 2：
Chicken is contained with probiotics feed per 1000g chickens probiotics feed：Corn 721, dregs of beans 60g, wheat bran It is 80g, cotton dregs 10g, glutamic acid slag 10g, vinasse 80g, whitewashing maize peel 10g, bluestone powder 10g, medical stone 5g, soya-bean oil 1g, compound Probiotics 2g, 70% lysine 1g, calcium bicarbonate 5g, methionine 1g, composite mineral matter 3g, multi-vitamins 1g.
The compound micro-ecological preparation presses 2 by Paula enlightening saccharomycete bacterium powder and lactobacillus acidophilus bacterium powder:1 mass ratio group Into wherein the viable count of Paula enlightening saccharomycete bacterium powder is 1.0 × 1010CFU/g-2.5×1010CFU/g, lactobacillus acidophilus bacterium powder Viable count be 1.0 × 1011CFU/g-3.0×1011CFU/g。
The preparation method of chicken of the present invention probiotics feed,
1) prepared by Paula enlightening yeast bacterium powder：
Take fresh honey raisin tree to be beaten to obtain honey raisin tree slurry after cleaning, the fruit of honey raisin tree slurry quality 0.5% is separately added into being starched to honey raisin tree Glue enzyme, cellulase and hemicellulase keep the temperature 3h in 60 DEG C, then filter and obtain hovenia acerba juice, are cooled to after 39 DEG C by volume The Paula enlightening yeast activated of access 2%, after 37 DEG C of constant temperature incubation 30h, at 5 DEG C, 5000rpm centrifugations 15min, which is collected, to be protected Lardy yeast bacterium mud, then add 2 times of weight of bacterium mud compound freeze drying protectant be placed in vacuum freeze drier freeze it is dry Dry 24h, is 3.0 × 10 up to viable count10CFU/g-5.0×1010The Paula enlightening saccharomycete bacterium powder of CFU/g；
The 100g compound freeze drying protectant 12g containing synanthrin, trehalose 10g, threonine 0.6g, methionine 3g, chlorination Sodium 2g, phosphate buffer 25g, distilled water 47.4g；
2) prepared by lactobacillus acidophilus bacterium powder：
Added in puncture vine extracting solution the glucose of puncture vine extracting solution quality 1.5%, 0.3% casein hydrolysate, 0.3% sodium acetate and 0.3% diammonium hydrogen citrate, then access lactobacillus acidophilus, in 37 DEG C of constant temperature by 3% volume ratio After cultivating 20h, in 6 DEG C, 7000rpm centrifugations 15min obtains lactobacillus acidophilus bacterium mud, then adds the compound of 2 times of bacterium mud weight Freeze drying protectant is placed on vacuum freeze drier drying 24h, is 2.1 × 10 up to viable count11CFU/g-6.0×1011CFU/ The lactobacillus acidophilus bacterium powder of g；
Every the 100g compound freeze drying protectant 3g containing phytase, galactooligosaccharide 12g, hovenia polysaccharides 4g, soya-bean oil 2g, rely Propylhomoserin 2g, choline chloride 5g, phosphate buffer 22g, distilled water 50g；
3) Paula enlightening saccharomycete bacterium powder and lactobacillus acidophilus bacterium powder are pressed 2:1 mass ratio is mixed and made into compound microecological system Agent；
4) corn 721, dregs of beans 60g, wheat bran 80g, cotton dregs 10g, glutamic acid slag 10g, vinasse 80g, spray are added in every 1000g Starch maize peel 10g, bluestone powder 10g, medical stone 5g, soya-bean oil 1g, compound micro-ecological preparation 2g, 70% lysine 1g, calcium bicarbonate 5g, methionine 1g, composite mineral matter 3g, multi-vitamins 1g obtain chicken probiotics feed.
The preparation method of the puncture vine extracting solution is：Puncture vine is taken by 1：14 mass ratio soaks 25min, then heats Boil and maintain boiling 30min, then with 400 mesh screens, filtered after cooling with vacuum pump and add deionized water afterwards twice and make Puncture vine and water ratio are 1:10 up to puncture vine extracting solution.
The preparation method of the hovenia polysaccharides is：The supernatant being collected by centrifugation after the enlightening yeast bacterium mud of Paula of step 1) is passed through Low temperature is concentrated into the absolute ethyl alcohol that 3 times of volumes are added after 1/3, stands 3h and filters to obtain honey raisin tree Thick many candies, then honey raisin tree Thick many candies are answered Chloroform and n-butanol removing protein are added after water, then water intaking mutually adds chloroform and n-butanol again, three times by cold after de- albumen Freeze dry acquisition hovenia polysaccharides.
Embodiment 3：
Chicken is contained with probiotics feed per 1000g chickens probiotics feed：Corn 632g, dregs of beans 80g, wheat bran It is 100g, cotton dregs 20g, glutamic acid slag 20g, vinasse 90g, whitewashing maize peel 15g, bluestone powder 15g, medical stone 7g, soya-bean oil 2g, compound Probiotics 3g, 70% lysine 1.5g, calcium bicarbonate 7g, methionine 1.5g, composite mineral matter 4g, multi-vitamins 2g.
The compound micro-ecological preparation presses 1 by Paula enlightening saccharomycete bacterium powder and lactobacillus acidophilus bacterium powder:2 mass ratio group Into wherein the viable count of Paula enlightening saccharomycete bacterium powder is 1.0 × 1010CFU/g-2.5×1010CFU/g, lactobacillus acidophilus bacterium powder Viable count be 1.0 × 1011CFU/g-3.0×1011CFU/g。
The preparation method of chicken of the present invention probiotics feed,
1) prepared by Paula enlightening yeast bacterium powder：
Take fresh honey raisin tree to be beaten to obtain honey raisin tree slurry after cleaning, the fruit of honey raisin tree slurry quality 0.8% is separately added into being starched to honey raisin tree Glue enzyme, cellulase and hemicellulase keep the temperature 2.5h in 60 DEG C, then filter and obtain hovenia acerba juice, are cooled to after 40 DEG C by volume Than the Paula enlightening yeast activated of access 5%, after 37 DEG C of constant temperature incubation 36h, at 8 DEG C, 6000rpm centrifugations 10min is collected Paula enlightening yeast bacterium mud, then the compound freeze drying protectant of addition 2 times of weight of bacterium mud, which is placed in vacuum freeze drier, freezes Dry 24h, is 3.0 × 10 up to viable count10CFU/g-5.0×1010The Paula enlightening saccharomycete bacterium powder of CFU/g；
The 100g compound freeze drying protectant 15g containing synanthrin, trehalose 12g, threonine 0.8g, methionine 5g, chlorination Sodium 2.8g, phosphate buffer 21.5g, distilled water 42.9g；
2) prepared by lactobacillus acidophilus bacterium powder：
The glucose of addition puncture vine extracting solution quality 2%, 0.4% casein hydrolysate, 0.2% in puncture vine extracting solution Sodium acetate and 0.4% diammonium hydrogen citrate, then by 5% volume ratio access lactobacillus acidophilus, in 37 DEG C of constant temperature incubations After 24h, in 8 DEG C, 8000rpm centrifugations 10min obtains lactobacillus acidophilus bacterium mud, then adds the compound lyophilized of 1 times of bacterium mud weight Protective agent is placed on vacuum freeze drier drying 24h, is 2.1 × 10 up to viable count11CFU/g-6.0×1011CFU/g's Lactobacillus acidophilus bacterium powder；
Every the 100g compound freeze drying protectant 2g containing phytase, galactooligosaccharide 10g, hovenia polysaccharides 3g, soya-bean oil 1g, rely Propylhomoserin 1g, choline chloride 3g, phosphate buffer 20g, distilled water 60g；
3) Paula enlightening saccharomycete bacterium powder and lactobacillus acidophilus bacterium powder are pressed 1:2 mass ratio is mixed and made into compound microecological system Agent；
4) per add in 1000g corn 632g, dregs of beans 80g, wheat bran 100g, cotton dregs 20g, glutamic acid slag 20g, vinasse 90g, Whitewash maize peel 15g, bluestone powder 15g, medical stone 7g, soya-bean oil 2g, compound micro-ecological preparation 3g, 70% lysine 1.5g, carbonic acid Hydrogen calcium 7g, methionine 1.5g, composite mineral matter 4g, multi-vitamins 2g obtain chicken probiotics feed.
The preparation method of the puncture vine extracting solution is：Puncture vine is taken by 1：14 mass ratio soaks 25min, then heats Boil and maintain boiling 30min, then with 400 mesh screens, filtered after cooling with vacuum pump and add deionized water afterwards twice and make Puncture vine and water ratio are 1:10 up to puncture vine extracting solution.
The preparation method of the hovenia polysaccharides is：The supernatant being collected by centrifugation after the enlightening yeast bacterium mud of Paula of step 1) is passed through Low temperature is concentrated into the absolute ethyl alcohol that 3 times of volumes are added after 1/3, stands 3h and filters to obtain honey raisin tree Thick many candies, then honey raisin tree Thick many candies are answered Chloroform and n-butanol removing protein are added after water, then water intaking mutually adds chloroform and n-butanol again, three times by cold after de- albumen Freeze dry acquisition hovenia polysaccharides.

Claims (5)

1. breeder probiotics feed, it is characterised in that：Contained per 1000g chickens with probiotics feed：Corn 544- 721g, dregs of beans 60-100g, wheat bran 80-120g, cotton dregs 10-30g, glutamic acid slag 10-30g, vinasse 80-100g, whitewashing maize peel 10-20g, bluestone powder 10-20g, medical stone 5-10g, soya-bean oil 1-3g, compound micro-ecological preparation 2-5g, 70% lysine 1-2g, carbon Sour hydrogen calcium 5-7g, methionine 1-2g, composite mineral matter 3-5g, multi-vitamins 1-3g.
2. chicken according to claim 1 probiotics feed, it is characterised in that：The compound micro-ecological preparation is by protecting Lardy saccharomycete bacterium powder and lactobacillus acidophilus bacterium powder press 2:1~1:2 mass ratio composition, the wherein work of Paula enlightening saccharomycete bacterium powder Bacterium number is 1.0 × 1010CFU/g-2.5×1010CFU/g, the viable count of lactobacillus acidophilus bacterium powder are 1.0 × 1011CFU/g-3.0 ×1011CFU/g。
3. the breeder preparation method of probiotics feed, it is characterised in that：
1) prepared by Paula enlightening yeast bacterium powder：
Take fresh honey raisin tree to be beaten to obtain honey raisin tree slurry after cleaning, the pectin of honey raisin tree slurry quality 0.5-1% is separately added into being starched to honey raisin tree Enzyme, cellulase and hemicellulase keep the temperature 2-3h in 60 DEG C, then filter and obtain hovenia acerba juice, by body after being cooled to 38-40 DEG C The Paula enlightening yeast that has activated of the product than accessing 1-5%, after 37 DEG C of constant temperature incubation 28-36h, at 4-8 DEG C, 4000-6000rpm Centrifuge 10-20min and collect Paula enlightening yeast bacterium mud, the compound freeze drying protectant for then adding 2 times of weight of bacterium mud is placed on vacuum 24h is freeze-dried in freeze drier, is up to viable count
3.0×1010CFU/g-5.0×1010The Paula enlightening saccharomycete bacterium powder of CFU/g；
The 100g compound freeze drying protectant 10-15g containing synanthrin, trehalose 8-12g, threonine 0.4-0.8g, methionine 2- 5g, sodium chloride 2-2.8g, phosphate buffer 20-25g, distilled water 42.9-57.6g；
2) prepared by lactobacillus acidophilus bacterium powder：
Added in puncture vine extracting solution the glucose of puncture vine extracting solution quality 1-2%, 0.2-0.4% casein hydrolysate, The sodium acetate of 0.1-0.3% and the diammonium hydrogen citrate of 0.2-0.4%, then access lactobacillus acidophilus by the volume ratio of 2-5%, After 37 DEG C of constant temperature incubation 18-24h, in 4-8 DEG C, 6000-8000rpm centrifugations 10-20min obtains lactobacillus acidophilus bacterium mud, so The compound freeze drying protectant for adding 1-3 times of bacterium mud weight afterwards is placed on vacuum freeze drier drying 24h, is up to viable count 2.1×1011CFU/g-6.0×1011The lactobacillus acidophilus bacterium powder of CFU/g；
Every the 100g compound freeze drying protectant 2-5g containing phytase, galactooligosaccharide 10-15g, hovenia polysaccharides 3-5g, soya-bean oil 1- 3g, lysine 1-3g, choline chloride 3-5g, phosphate buffer 20-25g, distilled water 41-60g；
3) Paula enlightening saccharomycete bacterium powder and lactobacillus acidophilus bacterium powder are pressed 2:1~1:2 mass ratio is mixed and made into compound microecological Preparation；
4) corn 544-721g, dregs of beans 60-100g, wheat bran 80-120g, cotton dregs 10-30g, glutamic acid slag are added in every 1000g It is 10-30g, vinasse 80-100g, whitewashing maize peel 10-20g, bluestone powder 10-20g, medical stone 5-10g, soya-bean oil 1-3g, compound micro- Ecological agent 2-5g, 70% lysine 1-2g, calcium bicarbonate 5-7g, methionine 1-2g, composite mineral matter 3-5g, multi-vitamins 1-3g obtains chicken probiotics feed.
A kind of 4. preparation method of chicken probiotics feed as claimed in claim 3, it is characterised in that：The puncture vine carries The preparation method for taking liquid is：Puncture vine is taken by 1：14 mass ratio soaks 25min, and then heating boils and maintains to seethe with excitement 30min, then with 400 mesh screens, is filtered with vacuum pump and adds deionized water afterwards twice and make puncture vine and the water ratio be after cooling 1:10 up to puncture vine extracting solution.
A kind of 5. preparation method of chicken probiotics feed as claimed in claim 3, it is characterised in that：The honey raisin tree is more Sugar preparation method be：By the supernatant being collected by centrifugation after the enlightening yeast bacterium mud of Paula of step 1) after low temperature is concentrated into 1/3 plus Enter the absolute ethyl alcohol of 3 times of volumes, stand 3h and filter to obtain honey raisin tree Thick many candies, then chloroform and positive fourth will be added after honey raisin tree Thick many candies rehydration Alcohol removing protein, then water intaking mutually add chloroform and n-butanol again, obtain hovenia polysaccharides by being freeze-dried after de- albumen three times.
CN201711487585.4A 2017-12-30 2017-12-30 One breeder probiotics feed and preparation method thereof Pending CN108013261A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108611303A (en) * 2018-05-16 2018-10-02 陕西科技大学 A kind of preparation method except the prebiotic bacteria adsorbent of cadmium and its application in vegetable juice
CN108651810A (en) * 2018-05-16 2018-10-16 陕西科技大学 A kind of preparation method of prebiotic bacteria adsorbent and its application that cadmium is removed in fruit juice

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008013534A (en) * 2006-07-07 2008-01-24 Health Vision:Kk Probiotics product for pet animal
CN102258153A (en) * 2011-08-25 2011-11-30 山东六和集团有限公司 Compound feed for laying hen in egg-laying period and preparation method thereof
CN102358884A (en) * 2011-10-13 2012-02-22 陕西农产品加工技术研究院 Bifidobacterium bifidum lyophilization composite protection agent and application method thereof
WO2013036715A1 (en) * 2011-09-07 2013-03-14 Massoud Arvanaghi Capsaicin composition and method related thereto
CN103478410A (en) * 2013-09-16 2014-01-01 陕西科技大学 Probiotic protein feed produced through peltate yam rhizomes and production method thereof
CN103478411A (en) * 2013-09-16 2014-01-01 陕西科技大学 Turmeric starch probiotics activity feed and preparing method thereof
CN103494035A (en) * 2013-09-23 2014-01-08 合肥市益农养殖有限公司 Chicken feed for improving survival rate
CN103820300A (en) * 2014-03-07 2014-05-28 余芳 Manufacturing method of turnjujube fruit vinegar
CN103923835A (en) * 2014-04-25 2014-07-16 陕西科技大学 Method for preparing lactobacillus bulgaricus freeze-dried powder
CN104171538A (en) * 2014-07-07 2014-12-03 辽宁禾丰牧业股份有限公司 Saving-type daily ration for laying hens during egg laying peak and preparation method thereof
CN105420111A (en) * 2015-12-30 2016-03-23 陕西科技大学 Composite protective agent for increasing freeze-drying survival rate of lactobacillus plantarum and preparation method thereof
CN105685223A (en) * 2016-02-01 2016-06-22 陕西科技大学 Preparation method of antioxidant lactobacillus acidophilus fermented goat milk
CN106234589A (en) * 2016-09-23 2016-12-21 陕西科技大学 A kind of preparation method of antioxidation probiotics fermention breast

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008013534A (en) * 2006-07-07 2008-01-24 Health Vision:Kk Probiotics product for pet animal
CN102258153A (en) * 2011-08-25 2011-11-30 山东六和集团有限公司 Compound feed for laying hen in egg-laying period and preparation method thereof
WO2013036715A1 (en) * 2011-09-07 2013-03-14 Massoud Arvanaghi Capsaicin composition and method related thereto
CN102358884A (en) * 2011-10-13 2012-02-22 陕西农产品加工技术研究院 Bifidobacterium bifidum lyophilization composite protection agent and application method thereof
CN103478410A (en) * 2013-09-16 2014-01-01 陕西科技大学 Probiotic protein feed produced through peltate yam rhizomes and production method thereof
CN103478411A (en) * 2013-09-16 2014-01-01 陕西科技大学 Turmeric starch probiotics activity feed and preparing method thereof
CN103494035A (en) * 2013-09-23 2014-01-08 合肥市益农养殖有限公司 Chicken feed for improving survival rate
CN103820300A (en) * 2014-03-07 2014-05-28 余芳 Manufacturing method of turnjujube fruit vinegar
CN103923835A (en) * 2014-04-25 2014-07-16 陕西科技大学 Method for preparing lactobacillus bulgaricus freeze-dried powder
CN104171538A (en) * 2014-07-07 2014-12-03 辽宁禾丰牧业股份有限公司 Saving-type daily ration for laying hens during egg laying peak and preparation method thereof
CN105420111A (en) * 2015-12-30 2016-03-23 陕西科技大学 Composite protective agent for increasing freeze-drying survival rate of lactobacillus plantarum and preparation method thereof
CN105685223A (en) * 2016-02-01 2016-06-22 陕西科技大学 Preparation method of antioxidant lactobacillus acidophilus fermented goat milk
CN106234589A (en) * 2016-09-23 2016-12-21 陕西科技大学 A kind of preparation method of antioxidation probiotics fermention breast

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
舒国伟，等: "蒺藜和嗜酸乳杆菌相互作用的研究", 《中国酿造》 *
陈合，等: "SCREENING FREEZE-DRYING CRYOPROTECTANTS FOR SACCHAROMYCES BOULARDII BY PLACKETT-BURMAN DESIGN", 《THE ANNALS OF THE UNIVERSITY DUNAREA DE JOS OF GALATI FASCICLE VI–FOOD TECHNOLOGY》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108611303A (en) * 2018-05-16 2018-10-02 陕西科技大学 A kind of preparation method except the prebiotic bacteria adsorbent of cadmium and its application in vegetable juice
CN108651810A (en) * 2018-05-16 2018-10-16 陕西科技大学 A kind of preparation method of prebiotic bacteria adsorbent and its application that cadmium is removed in fruit juice

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Application publication date: 20180511