CN108651810A - A kind of preparation method of prebiotic bacteria adsorbent and its application that cadmium is removed in fruit juice - Google Patents
A kind of preparation method of prebiotic bacteria adsorbent and its application that cadmium is removed in fruit juice Download PDFInfo
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- CN108651810A CN108651810A CN201810468289.8A CN201810468289A CN108651810A CN 108651810 A CN108651810 A CN 108651810A CN 201810468289 A CN201810468289 A CN 201810468289A CN 108651810 A CN108651810 A CN 108651810A
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- 229910052793 cadmium Inorganic materials 0.000 title claims abstract description 107
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 title claims abstract description 107
- 241000894006 Bacteria Species 0.000 title claims abstract description 78
- 235000015203 fruit juice Nutrition 0.000 title claims abstract description 49
- 239000003463 adsorbent Substances 0.000 title claims abstract description 42
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- 238000002360 preparation method Methods 0.000 title claims abstract description 23
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- 235000018291 probiotics Nutrition 0.000 claims abstract description 29
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- 238000004108 freeze drying Methods 0.000 claims abstract description 25
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- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000001814 pectin Substances 0.000 claims abstract description 9
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- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 7
- 108010059892 Cellulase Proteins 0.000 claims description 7
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/80—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by adsorption
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
Abstract
A kind of preparation method of prebiotic bacteria adsorbent and its application that cadmium is removed in fruit juice, it per g lactobacillus bacterium mud with the mixing of the ratio of the compound freeze drying protectant of 1 3g, viable count is 1.2 × 10 that prebiotic bacteria adsorbent, which is by lactobacillus and compound freeze drying protectant,11CFU/g‑6.5×1011CFU/g；Prebiotic bacteria adsorbent is added by the additive amount of 1 5g/L in fruit juice, then standing adsorption removes 2~4h of cadmium at 37 DEG C, and cadmium removal rate is 95 100%, and then refrigerated centrifuge is to get no cadmium fruit juice and the lactobacillus bacterium mud containing cadmium.The present invention had both solved the problems, such as that the heavy metal pollution of fruit juice and safety were drunk, and implemented convenient, fast, efficiently.Lactobacillus, which is added to the lactic acid that proper fermentation generates in fruit juice, can increase the flavor substance of fruit juice, enhance mouthfeel.Pectin, chitosan and the zeolite added in compound freeze drying protectant also has cadmium the ability of absorption cadmium, collaboration probiotics stronger except cadmium ability.
Description
Technical field
The invention belongs to field of food, more particularly to the preparation method of a kind of prebiotic bacteria adsorbent and its in fruit juice remove cadmium
Application.
Background technology
With the rapid development of industrial or agricultural, the trade effluent of mining wastewater, plating and battery is untreated or processing is not up to standard
Sewage irrigation, using the fertilising containing cadmium element and the industrial waste gas containing cadmium pollution through drench with rain with the modes such as natural subsidence into
Enter soil.The cadmium of soil into plant and is accumulated into vegetables by the effect of vegetation root absorption.Cadmium is FAO (Food and Agriculture Organization of the United Nation)
(FAO) and the World Health Organization (WHO) announce strongest 3 heavy metal species of human toxicity (lead, cadmium, mercury) in a kind, in human body
Interior deposition is latent to be 10~30 years.Cadmium slow poisoning is easiest to cause the organs such as kidney and liver that lesion occurs, and can also cause
Itai-itai diseases, hypertension, diabetes, anaemia, tumour etc., long-term cadmium of taking in have certain carcinogenic disease, the harm to children's health
It is especially big.
Currently, the method for removal cadmium mainly has：One, Physical.This method application low-frequency ultrasonic waves, cold and hot water coke slurry and absorption
Etc. technologies, and assist with polish the processing means such as epidermis, cleaning and rinsing remove cadmium, due to vegetable species difference, to cadmium from
The binding ability of son is different, and the Scavenging activity of Physical is limited；Two, chemical method.The method application resin, tannin, sodium citrate and
The reagents such as sodium oxalate by it is organic catch, plasma exchanges, forms the methods of complex compound, chelation and microprecipitation removes food
In cadmium, but the use of some toxic chemicals may be formed in food residual endanger human health；Three, biological
Method.The method is mainly to be removed in food by the absorption or cumulative effect of the microorganisms such as plant and its extract, fungi and bacterium
Cadmium, bioanalysis have safety, noresidue, free of contamination advantage.
Research shows that lactic acid bacteria has scavenging effect, possible work to the cadmium in the food such as water, grain, fruits and vegetables
It is that the free cadmium ion without metabolism is attached to cell wall table by positive and negative charge by cell surface suction-operated with mechanism
Face, cell wall are mainly made of peptide glycan, teichoic acid and neutral polysaccharide etc., and the reticular structure of teichoic acid and polysaccharide formation is conducive to
Adhesion and absorption cadmium.The protein on lactobacillus cell surface possesses a large amount of negatively charged functional groups, can effectively combine metal
Cation.The lactic acid that lactic acid bacteria metabolism generates is formed by acidic environment has solubilization, lactic acid bacteria metabolism to cadmium complex
The macromolecular substances such as polysaccharide, polypeptide and nucleic acid of formation surface carries COO-、HPO4-And OH-Equal groups, can occur with cadmium ion
Electrostatic adsorption combines Cd as the S-Layer albumen of Lactobacillus brevis cell surfaces has2+Ability.Mesh
Preceding discovery can remove the lactic acid bacteria of cadmium mainly and include Lactobacillus, Pediococcus, Enterococcus and
The categories such as Bifidobacterium.Bhakta etc. is filtered out from fish enteron aisle with good adherence power bacterial strain L.reuteri Cd70-
13, it is 25% to the cadmium clearance rate in MRS culture mediums.Zhai etc. utilizes bacterial strain Lactobacillus
PlantarumCCFM8610 can effectively remove in initial concentration 5mg/L Cd aqueous solutions 31.34% cadmium.The researchs such as Halttunen
It was found that bacterial strain Bifidobacterium longum 46 can remove the cadmium of dry weight 54.7mg/g in water.Topcu etc. research shows that
Bacterial strain E.faecium EF031 and E.faecium M74 can effectively remove cadmium in water, remove range be respectively 77.3%~
98.1% and 53.5%~91.0%.The researchs such as Halttunen find that bacterial strain L.rhamnosus GG pass through cell surface electrostatic
In phosphate buffer (PBS) 22.1%~49.1% cadmium is removed in suction-operated.Fu etc. application L.plantarum and
P.pentosaceus presses 2:After 1 mixing, with 3% inoculum concentration, the 23.4h that ferments at 40.8 DEG C can effectively be removed in rice
85.73% cadmium.Research at present using cadmium in lactic acid bacteria removal fruit juice is few, therefore screening is resistant in cadmium and removal fruit juice
The prebiotic bacteria adsorbent of heavy metal cadmium is necessary.
Invention content
The application of cadmium is removed the purpose of the present invention is to provide a kind of preparation method of prebiotic bacteria adsorbent and its in fruit juice.
To achieve the goals above, the preparation method of the prebiotic bacteria adsorbent of the present invention is as follows：
1) preparation of compound freeze drying protectant
The pectin of 0.5-2g, the distillation of the chitosan of 3-5g, the zeolite of 0.3-0.8g and 12.2-16.2g are added by every 20g
Water is mixed to get compound freeze drying protectant；
2) preparation of probiotics bacterium powder
Fresh honey raisin tree is beaten after cleaning, and the activity that honey raisin tree quality 0.08% is added in being starched to honey raisin tree is 30000U/g
Pectase, 0.15% active 8000U/g cellulase add with 0.75% activity be 10000U/g hemicellulase
2-3h is kept the temperature in 60 DEG C, then filters and obtains hovenia acerba juice, then the casein of 2~5g/L is added by every liter of hovenia acerba juice into hovenia acerba juice
Peptone, 1.5-2.0g/L synanthrin after sterilize 20 minutes in 121 DEG C, be cooled to after 38-40 DEG C and the leucine of membrane filtration and small be added
Cow's serum, make the final concentration of leucine and calf serum be respectively 10~16mg/L and 1-3g/L, after access 1-5% by volume
The lactobacillus activated, in 37 DEG C of constant temperature incubation 20-24h, then refrigerated centrifuge collects thalline and obtains probiotics bacterium powder；
3) the compound freeze drying protectant of 1-3 times of weight is added into probiotics bacterium powder, is uniformly mixed with whirlpool instrument, is then adopted
With vacuum freeze drying 20-24h, it is 1.2 × 10 to obtain viable count11CFU/g-6.5×1011The prebiotic bacteria adsorbent of CFU/g.
The lactobacillus is one or both of lactobacillus acidophilus, Lactobacillus rhamnosus.
The application of cadmium is removed in fruit juice by prebiotic bacteria adsorbent made from preparation method of the present invention：
First by fresh fruit cleaning, destemming, squeeze the juice processing after according to 1：2 mass ratioes add water, are then centrifuged for removal precipitation,
Sterilize in high-pressure steam sterilizing pan at 105 DEG C 10min, is centrifuged off coarse sludge, adds cadmium salt, and it is 2-5mg/ to make cadmium concentration
L, then fruit juice pH is adjusted to 6~7, then prebiotic bacteria adsorbent is added in fruit juice, additive amount 1-5g/L, then in 37 DEG C
Lower standing adsorption removes 2~4h of cadmium, and cadmium removal rate is 95-100%, and then refrigerated centrifuge is to get no cadmium fruit juice and the newborn bar containing cadmium
Bacterium bacterium mud.
The fruit juice is cider, grape juice, kiwi-fruit juice, juice of my pomegranate, pear juice, mango juice, watermelon juice, Cranberry
Juice, orange juice, coconut milk, Cantaloupe juice, strawberry juice, Chinese flowering quince juice or cherry juice.
The lactobacillus bacterium mud containing cadmium recycles, and is suspended into physiological saline, and it is 2.5- then to adjust pH
4.0, it is stored at room temperature after 0.5-1h to centrifuge and discharges the cadmium of absorption into supernatant, be precipitated as prebiotic bacteria adsorbent.
Prebiotic bacteria adsorbent after circular treatment of the present invention, which can be again added in fruit juice, to be recycled except cadmium, is recycled 5 times
Afterwards, probiotics bacterium mud is used for feed addictive.
The effect of invention
1) it is both to have solved the heavy metal pollution of fruit juice except cadmium agent to utilize microorganism-lactobacillus of generally recognized as safe (GRAS)
Problem is drunk with safety, and implements convenient, fast, efficiently.
2) flavor substance of fruit juice can be increased by lactobacillus being added to the lactic acid that proper fermentation generates in fruit juice, enhance mouth
Sense.
3) pectin, chitosan and the zeolite added in compound freeze drying protectant also has cadmium the ability of absorption cadmium, collaboration
Probiotics is stronger except cadmium ability.
4) when cadmium concentration is 2-5mg/L in fruit juice, cadmium removal rate is more than 95%.
Specific implementation mode
It is further illustrated the present invention with reference to embodiment：
Embodiment 1：
1) preparation of compound freeze drying protectant
The pectin of 2g is added by every 20g, the distilled water of the chitosan of 5g, the zeolite of 0.8g and 12.2g is mixed to get compound
Freeze drying protectant；
2) preparation of probiotics bacterium powder
Fresh honey raisin tree is beaten after cleaning, and the activity that honey raisin tree quality 0.08% is added in being starched to honey raisin tree is 30000U/g
Pectase, 0.15% active 8000U/g cellulase add with 0.75% activity be 10000U/g hemicellulase
Keep the temperature 2h in 60 DEG C, then filter and obtain hovenia acerba juice, then into hovenia acerba juice by every liter of hovenia acerba juice be added 2g/L casein peptone,
It sterilizes 20 minutes in 121 DEG C after the synanthrin of 1.5g/L, is cooled to the leucine and calf serum that membrane filtration is added after 38 DEG C, makes bright
The final concentration of propylhomoserin and calf serum be respectively 10mg/L and 1g/L, after access by volume 2% the acidophilus breast bar activated
Bacterium strain, in 37 DEG C of constant temperature incubation 20h, then refrigerated centrifuge collects thalline and obtains probiotics bacterium powder；
3) the compound freeze drying protectant of 3 times of weight is added into probiotics bacterium powder, is uniformly mixed with whirlpool instrument, is then used
Vacuum freeze drying 20h, it is 1.2 × 10 to obtain viable count11The prebiotic bacteria adsorbent of CFU/g.
Prebiotic bacteria adsorbent removes the application of cadmium in fruit juice,：First by fresh apple cleaning, destemming, squeeze the juice processing after by
According to 1：2 mass ratioes add water, are then centrifuged for removal precipitation, sterilize in high-pressure steam sterilizing pan at 105 DEG C 10min, is centrifuged off
Coarse sludge adds cadmium salt, and it is 5mg/L to make cadmium concentration, then fruit juice pH is adjusted to 6, and prebiotic bacteria adsorbent is then added to fruit
In juice, additive amount 1g/L, then at 37 DEG C standing adsorption remove cadmium 4h, cadmium removal rate be 95%, then refrigerated centrifuge to get
Lactobacillus bacterium mud without cadmium fruit juice and containing cadmium.
The obtained lactobacillus bacterium mud containing cadmium is suspended in physiological saline, it is 2.5 then to adjust pH, is stored at room temperature 0.5h
Centrifugation (8000rpm, 10min) discharges the cadmium of absorption into supernatant afterwards, is precipitated as prebiotic bacteria adsorbent, it is added again
To being recycled in fruit juice except cadmium, after recycling 5 times, probiotics bacterium mud is used for feed addictive.
Embodiment 2：
1) preparation of compound freeze drying protectant
The pectin of 0.5g is added by every 20g, the distilled water of the chitosan of 3.5g, the zeolite of 0.5g and 15.5g is mixed to get
Compound freeze drying protectant；
2) preparation of probiotics bacterium powder
Fresh honey raisin tree is beaten after cleaning, and the activity that honey raisin tree quality 0.08% is added in being starched to honey raisin tree is 30000U/g
Pectase, 0.15% active 8000U/g cellulase add with 0.75% activity be 10000U/g hemicellulase
Keep the temperature 2.5h in 60 DEG C, then filter and obtain hovenia acerba juice, then into hovenia acerba juice by every liter of hovenia acerba juice be added 3g/L casein peptone,
It sterilizes 20 minutes in 121 DEG C after the synanthrin of 1.6g/L, is cooled to the leucine and calf serum that membrane filtration is added after 40 DEG C, makes bright
The final concentration of propylhomoserin and calf serum be respectively 12mg/L and 1.5g/L, after access by volume 2.5% the sandlwood activated
Sugared lactobacillus strain, in 37 DEG C of constant temperature incubation 22h, then refrigerated centrifuge collects thalline and obtains probiotics bacterium powder；
3) the compound freeze drying protectant of 1 times of weight is added into probiotics bacterium powder, is uniformly mixed with whirlpool instrument, is then used
Vacuum freeze drying 20h, it is 1.6 × 10 to obtain viable count11The prebiotic bacteria adsorbent of CFU/g.
Prebiotic bacteria adsorbent removes the application of cadmium in fruit juice,：First by new fresh grape cleaning, destemming, squeeze the juice processing after by
According to 1：2 mass ratioes add water, are then centrifuged for removal precipitation, sterilize in high-pressure steam sterilizing pan at 105 DEG C 10min, is centrifuged off
Coarse sludge adds cadmium salt, and it is 2mg/L to make cadmium concentration, then fruit juice pH is adjusted to 6.5, is then added to prebiotic bacteria adsorbent
In fruit juice, additive amount 3g/L, then standing adsorption removes cadmium 3h at 37 DEG C, and cadmium removal rate is 100%, then refrigerated centrifuge,
Up to no cadmium fruit juice and the lactobacillus bacterium mud containing cadmium.
The obtained lactobacillus bacterium mud containing cadmium is suspended in physiological saline, it is 3.0 then to adjust pH, is stored at room temperature
(8000rpm, 10min) is centrifuged after 0.75h to discharge the cadmium of absorption into supernatant, is precipitated as prebiotic bacteria adsorbent, again by it
Secondary be added in fruit juice recycles except cadmium, and after recycling 5 times, probiotics bacterium mud is used for feed addictive.
Embodiment 3：
1) preparation of compound freeze drying protectant
The pectin of 2g is added by every 20g, the distilled water of the chitosan of 4.5g, the zeolite of 0.8g and 12.7g is mixed to get again
Close freeze drying protectant；
2) preparation of probiotics bacterium powder
Fresh honey raisin tree is beaten after cleaning, and the activity that honey raisin tree quality 0.08% is added in being starched to honey raisin tree is 30000U/g
Pectase, 0.15% active 8000U/g cellulase add with 0.75% activity be 10000U/g hemicellulase
Keep the temperature 3h in 60 DEG C, then filter and obtain hovenia acerba juice, then into hovenia acerba juice by every liter of hovenia acerba juice be added 4g/L casein peptone,
It sterilizes 20 minutes in 121 DEG C after the synanthrin of 2g/L, is cooled to the leucine and calf serum that membrane filtration is added after 39 DEG C, makes bright ammonia
Acid and calf serum final concentration be respectively 16mg/L and 2g/L, after access by volume 3% the lactobacillus acidophilus activated
(strain ratio is 2 for strain and Lactobacillus rhamnosus strain:1), in 37 DEG C of constant temperature incubation 20h, then refrigerated centrifuge collects thalline
Obtain probiotics bacterium powder；
3) the compound freeze drying protectant of 2 times of weight is added into probiotics bacterium powder, is uniformly mixed with whirlpool instrument, is then used
Vacuum freeze drying 20h, it is 3.5 × 10 to obtain viable count11The prebiotic bacteria adsorbent of CFU/g.
Prebiotic bacteria adsorbent removes the application of cadmium in fruit juice：First by fresh Kiwi berry cleaning, destemming, squeeze the juice processing after by
According to 1：2 mass ratioes add water, are then centrifuged for removal precipitation, sterilize in high-pressure steam sterilizing pan at 105 DEG C 10min, is centrifuged off
Coarse sludge adds cadmium salt, and it is 4mg/L to make cadmium concentration, then fruit juice pH is adjusted to 6, and prebiotic bacteria adsorbent is then added to fruit
In juice, additive amount 4g/L, then at 37 DEG C standing adsorption remove cadmium 3h, cadmium removal rate be 99%, then refrigerated centrifuge to get
Lactobacillus bacterium mud without cadmium fruit juice and containing cadmium.
The obtained lactobacillus bacterium mud containing cadmium is suspended in physiological saline, it is 2.8 then to adjust pH, is stored at room temperature 0.6h
Centrifugation (8000rpm, 10min) discharges the cadmium of absorption into supernatant afterwards, is precipitated as prebiotic bacteria adsorbent, it is added again
To being recycled in fruit juice except cadmium, after recycling 5 times, probiotics bacterium mud is used for feed addictive.
Embodiment 4：
1) preparation of compound freeze drying protectant
The pectin of 1.5g is added by every 20g, the distilled water of the chitosan of 3g, the zeolite of 0.3g and 15.2g is mixed to get again
Close freeze drying protectant；
2) preparation of probiotics bacterium powder
Fresh honey raisin tree is beaten after cleaning, and the activity that honey raisin tree quality 0.08% is added in being starched to honey raisin tree is 30000U/g
Pectase, 0.15% active 8000U/g cellulase add with 0.75% activity be 10000U/g hemicellulase
Keep the temperature 3h in 60 DEG C, then filter and obtain hovenia acerba juice, then into hovenia acerba juice by every liter of hovenia acerba juice be added 5g/L casein peptone,
It sterilizes 20 minutes in 121 DEG C after the synanthrin of 1.8g/L, is cooled to the leucine and calf serum that membrane filtration is added after 39 DEG C, makes bright
The final concentration of propylhomoserin and calf serum be respectively 14mg/L and 3g/L, after access by volume 1% the acidophilus breast bar activated
Bacterium strain, for 24 hours in 37 DEG C of constant temperature incubations, then refrigerated centrifuge collects thalline and obtains probiotics bacterium powder；
3) the compound freeze drying protectant of 1.5 times of weight is added into probiotics bacterium powder, is uniformly mixed with whirlpool instrument, is then adopted
With vacuum freeze drying 22h, it is 5.2 × 10 to obtain viable count11The prebiotic bacteria adsorbent of CFU/g.
Prebiotic bacteria adsorbent removes the application of cadmium in fruit juice,：First by fresh pomegranate cleaning, destemming, squeeze the juice processing after by
According to 1：2 mass ratioes add water, are then centrifuged for removal precipitation, sterilize in high-pressure steam sterilizing pan at 105 DEG C 10min, is centrifuged off
Coarse sludge adds cadmium salt, and it is 3mg/L to make cadmium concentration, then fruit juice pH is adjusted to 7, and prebiotic bacteria adsorbent is then added to fruit
In juice, additive amount 5g/L, then at 37 DEG C standing adsorption remove cadmium 2h, cadmium removal rate be 98%, then refrigerated centrifuge to get
Lactobacillus bacterium mud without cadmium fruit juice and containing cadmium.
The obtained lactobacillus bacterium mud containing cadmium is suspended in physiological saline, it is 3.5 then to adjust pH, after being stored at room temperature 1h
Centrifugation (8000rpm, 10min) discharges the cadmium of absorption into supernatant, is precipitated as prebiotic bacteria adsorbent, is again added to
It is recycled in fruit juice except cadmium, after recycling 5 times, probiotics bacterium mud is used for feed addictive.
Embodiment 5：
1) preparation of compound freeze drying protectant
The pectin of 0.5g is added by every 20g, the distilled water of the chitosan of 3g, the zeolite of 0.3g and 16.2g is mixed to get again
Close freeze drying protectant；
2) preparation of probiotics bacterium powder
Fresh honey raisin tree is beaten after cleaning, and the activity that honey raisin tree quality 0.08% is added in being starched to honey raisin tree is 30000U/g
Pectase, 0.15% active 8000U/g cellulase add with 0.75% activity be 10000U/g hemicellulase
Keep the temperature 2h in 60 DEG C, then filter and obtain hovenia acerba juice, then into hovenia acerba juice by every liter of hovenia acerba juice be added 3.5g/L casein peptone,
It sterilizes 20 minutes in 121 DEG C after the synanthrin of 1.9g/L, is cooled to the leucine and calf serum that membrane filtration is added after 40 DEG C, makes bright
The final concentration of propylhomoserin and calf serum be respectively 15mg/L and 2.5g/L, after access by volume 5% the rhamnose activated
Lactobacillus strain, in 37 DEG C of constant temperature incubation 21h, then refrigerated centrifuge collects thalline and obtains probiotics bacterium powder；
3) the compound freeze drying protectant of 2.5 times of weight is added into probiotics bacterium powder, is uniformly mixed with whirlpool instrument, is then adopted
For 24 hours with vacuum freeze drying, it is 6.5 × 10 to obtain viable count11The prebiotic bacteria adsorbent of CFU/g.
Prebiotic bacteria adsorbent removes the application of cadmium in fruit juice,：First by fresh cherry cleaning, destemming, squeeze the juice processing after by
According to 1：2 mass ratioes add water, are then centrifuged for removal precipitation, sterilize in high-pressure steam sterilizing pan at 105 DEG C 10min, is centrifuged off
Coarse sludge adds cadmium salt, and it is 5mg/L to make cadmium concentration, then fruit juice pH is adjusted to 7, and prebiotic bacteria adsorbent is then added to fruit
In juice, additive amount 2g/L, then at 37 DEG C standing adsorption remove cadmium 3h, cadmium removal rate be 96%, then refrigerated centrifuge to get
Lactobacillus bacterium mud without cadmium fruit juice and containing cadmium.
The obtained lactobacillus bacterium mud containing cadmium is suspended in physiological saline, it is 4 then to adjust pH, be stored at room temperature after 1h from
The heart (8000rpm, 10min) discharges the cadmium of absorption into supernatant, is precipitated as prebiotic bacteria adsorbent, is again added to fruit
It is recycled in juice except cadmium, after recycling 5 times, probiotics bacterium mud is used for feed addictive.
Claims (5)
1. a kind of preparation method of prebiotic bacteria adsorbent, it is characterised in that：
1) preparation of compound freeze drying protectant
The pectin of 0.5-2g is added by every 20g, the distilled water of the chitosan of 3-5g, the zeolite of 0.3-0.8g and 12.2-16.2g is mixed
Conjunction obtains compound freeze drying protectant；
2) preparation of probiotics bacterium powder
The fruit that the activity that fresh honey raisin tree is beaten after cleaning, and honey raisin tree quality 0.08% is added in being starched to honey raisin tree is 30000U/g
Glue enzyme, 0.15% active 8000U/g cellulase add with 0.75% activity be 10000U/g hemicellulase in 60
DEG C heat preservation 2-3h, then filters and obtains hovenia acerba juice, then into hovenia acerba juice by every liter of hovenia acerba juice be added 2~5g/L casein peptone,
It sterilizes 20 minutes in 121 DEG C after the synanthrin of 1.5-2.0g/L, is cooled to after 38-40 DEG C and the leucine of membrane filtration and small ox blood is added
Clearly, make the final concentration of leucine and calf serum be respectively 10~16mg/L and 1-3g/L, after accessed 1-5% by volume
The lactobacillus of activation, in 37 DEG C of constant temperature incubation 20-24h, then refrigerated centrifuge collects thalline and obtains probiotics bacterium powder；
3) the compound freeze drying protectant of 1-3 times of weight is added into probiotics bacterium powder, is uniformly mixed with whirlpool instrument, then uses true
Vacuum freecing-dry 20-24h, it is 1.2 × 10 to obtain viable count11CFU/g-6.5×1011The prebiotic bacteria adsorbent of CFU/g.
2. the preparation method of prebiotic bacteria adsorbent according to claim 1, it is characterised in that：The lactobacillus is acidophilus
One or both of lactobacillus, Lactobacillus rhamnosus.
3. a kind of prebiotic bacteria adsorbent prepared such as claim 1 removes the application of cadmium in fruit juice, it is characterised in that：It first will be new
According to 1 after fresh fruit cleaning, destemming, processing of squeezing the juice：2 mass ratioes add water, removal precipitation are then centrifuged for, in high-pressure steam sterilizing pan
In sterilize at 105 DEG C 10min, be centrifuged off coarse sludge, add cadmium salt, it is 2-5mg/L to make cadmium concentration, then fruit juice pH is adjusted to
6~7, then prebiotic bacteria adsorbent is added in fruit juice, additive amount 1-5g/L, then standing adsorption removes cadmium 2 at 37 DEG C
~4h, cadmium removal rate are 95-100%, and then refrigerated centrifuge is to get no cadmium fruit juice and the lactobacillus bacterium mud containing cadmium.
4. prebiotic bacteria adsorbent according to claim 3 removes the application of cadmium in fruit juice, it is characterised in that：The fruit juice
For cider, grape juice, kiwi-fruit juice, juice of my pomegranate, pear juice, mango juice, watermelon juice, Cranberry Juice Cocktail, orange juice, coconut milk, Kazakhstan
Close melon juice, strawberry juice, Chinese flowering quince juice or cherry juice.
5. prebiotic bacteria adsorbent according to claim 3 removes the application of cadmium in fruit juice, it is characterised in that：Described contains cadmium
Lactobacillus bacterium mud recycle, be suspended into physiological saline, then adjust pH be 2.5-4.0, be stored at room temperature 0.5-1h
It centrifuges afterwards and discharges the cadmium of absorption into supernatant, be precipitated as prebiotic bacteria adsorbent.
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Application publication date: 20181016 |