CN101224022B

CN101224022B - Processing method for simultaneously preparing sesame oil and protein by water enzymatic extraction

Info

Publication number: CN101224022B
Application number: CN2007100564540A
Authority: CN
Inventors: 李喜宏; 李娜; 邱泼; 杨涛; 韩文凤; 胡云峰; 王朝臣
Original assignee: Tianjin University of Science and Technology
Current assignee: Tianjin University of Science and Technology
Priority date: 2007-01-15
Filing date: 2007-01-15
Publication date: 2012-10-31
Anticipated expiration: 2027-01-15
Also published as: CN101224022A

Abstract

The invention relates to an aqueous enzymatic extraction technique for extracting sesame oil and protein. The technique is divided into two states of alkali extraction and enzymolysis: dehulled sesames are first ground into syrup; the sesame syrup with certain quality is added with water according to the solid-to-liquid ratio of 1:8 and the pH value of the sesame syrup is adjusted to about 8.7 when the temperature is increased to 50 DEG C; after the sesame syrup is stirred by water bath for about 90min, the pH value is adjusted to 4.8-5.2 and the sesame syrup is added with about 0.4 percent of composite cellulose. After the enzymatic hydrolysis and enzymolysis are carried out for 140-170 min, centrifugal separation is done; due to the difference of settlement factor, clean oil, emulsion, protein hydrolyzate and sesame residue are represented in sequence from up to down; 20 min after the completion of emulsion breaking, the clean oil and emulsion layers are centrifuged at the temperature of 85 DEG C and then the sesame oil with the yield being 90 percent is obtained; excellent sesame protein with tiny denaturation is obtained after the centrifugation, and protein deposition, cooling, drying and collection are done after the pH value is adjusted. The technique comprises the pretreatment of the sesame and the determination of temperature, time, pH value and enzyme adding amount. The technique of the invention not only ensures high extracting yield of the sesame oil with the quality achieving the national standard of first grade sesame oil, but also thoroughly extracts all the sesame protein almost without denaturation.

Description

Aqueous enzymatic method prepares the process of sesame oil and protein simultaneously

Technical field

The present invention relates to a kind of sesame oil processing method, particularly a kind of aqueous enzymatic method prepares the process of sesame oil and protein simultaneously.

Background technology

The grease preparation method has milling process, water for method and lixiviation process, and wherein oil prodution industry is most widely used at present is lixiviation process.Since the nineties; The research of oil and albumen becomes the focus of domestic and international concern in the enzyme process separation oil plant; At present, aqueous enzymatic method has become a kind of emerging oil extracting methods, and it is to utilize hydrolase to act on the cell membrane of vegetable oil material cell; Make its degraded of breaking, thereby the oil droplet in the cell discharges and assemble to obtain a kind of method of grease at aqueous phase.

Summary of the invention

The process that the present invention provides a kind of aqueous enzymatic method to prepare sesame oil and protein simultaneously prepares sesame protein simultaneously, can fully utilize plant resources.

Content of the present invention

Aqueous enzymatic extraction sesame oil and protein process are divided into alkali to be carried and two stages of enzymolysis, existing that the process introduction is following, and the sesame of will peeling earlier grinds pulping; Take by weighing the sesame slurry of certain mass, add water at 1: 8, treat that temperature is elevated to 50 ℃ by solid-to-liquid ratio; Adjust pH to 8.7; Behind the about 90min of stirring in water bath, adjust pH to 4.8～5.2, the complex cellulase of adding 0.4%.Behind enzyme hydrolysis enzymolysis time 140～170min, centrifugation is because the difference of sedimentation coefficient; Be edible vegetable oil, emulsion, protein hydrolyzate, sesame residues from top to bottom successively; With edible vegetable oil and emulsion layer, centrifugal behind 85 ℃ of breakdown of emulsion 20min, just obtain the sesame oil of yield about 90%; The protein hydrolyzate adjust pH is centrifugal to protein solution isoelectric point 4.5 backs, and protein precipitation is collected after the freeze drying, obtains the minimum high-quality sesame protein of sex change.

Characteristics of the present invention: this method can prepare sesame oil and protein simultaneously, and energy consumption is little, and is pollution-free; Investment goods is simple; The prepared sesame oil of this method not only recovery rate is high, and quality reaches the standard of country-level sesame oil, and can all sesame proteins be extracted; Protein is sex change hardly, has reached the comprehensive utilization of sesame resource.

Description of drawings

Fig. 1 is the process method flow chart that aqueous enzymatic method prepares sesame oil and protein simultaneously

Specific embodiment

Aqueous enzymatic extraction sesame oil and protein are divided into alkali and carry and two stages of enzymolysis:

1 sesame of will peeling earlier grinds pulping, takes by weighing the sesame slurry of certain mass, adds water at 1: 8 by solid-to-liquid ratio; Treat that temperature is elevated to 50 ℃, about adjust pH to 8.7, behind the about 90min of stirring in water bath; Adjust pH to 4.8～5.2, the complex cellulase of adding about 0.4%.Behind enzyme hydrolysis enzymolysis time 140～170min, centrifugation, because the difference of sedimentation coefficient is edible vegetable oil, emulsion, protein hydrolyzate, sesame residues from top to bottom successively,

2 with edible vegetable oil and emulsion layer, centrifugal behind 85 ℃ of breakdown of emulsion 20min, just obtains the sesame oil of yield about 90%; The protein hydrolyzate adjust pH is centrifugal to protein solution isoelectric point 4.5 backs, and protein precipitation is collected after the freeze drying, obtains the minimum high-quality sesame protein of sex change.

Claims

1. aqueous enzymatic extraction sesame oil and protein process is characterized in that:

Be divided into alkali and carry and two stages of enzymolysis, technology is following:

The sesame of will peeling earlier grinds pulping, takes by weighing the sesame slurry of certain mass, adds water at 1: 8 by solid-to-liquid ratio; Treat that temperature is elevated to 50 ℃, with sodium hydrogen phosphate-sodium dihydrogen phosphate adjust pH to 8.7, behind the stirring in water bath 90min; Adjust pH to 4.8～5.2, the complex cellulase of adding 0.4%; Enzyme hydrolysis enzymolysis time 140～170min; Centrifugation is because the difference of sedimentation coefficient is edible vegetable oil, emulsion, protein hydrolyzate, sesame residues from top to bottom successively; With centrifugal behind edible vegetable oil and 85 ℃ of breakdown of emulsion 20min of emulsion layer, just obtain yield at 90% sesame oil; The protein hydrolyzate adjust pH is centrifugal to protein hydrolyzate isoelectric point 4.5 backs, and protein precipitation is collected after the freeze drying, obtains sesame protein.