CN101659714B - Method for extracting sodium heparin and co-producing amino acid - Google Patents
Method for extracting sodium heparin and co-producing amino acid Download PDFInfo
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- CN101659714B CN101659714B CN2009100189690A CN200910018969A CN101659714B CN 101659714 B CN101659714 B CN 101659714B CN 2009100189690 A CN2009100189690 A CN 2009100189690A CN 200910018969 A CN200910018969 A CN 200910018969A CN 101659714 B CN101659714 B CN 101659714B
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Abstract
The invention discloses a method for extracting sodium heparin and co-producing amino acid, comprising the following steps: adding refined salt accounting for 1-1.8 weight percent of an intestinal mucosa into small intestinal mucosa; regulating the pH value of a solution to 9.0-9.2; adding fresh porcine pancreas to carry out zymolysis and filtering; carrying out resin absorption, elution and deposition; and drying to obtain the sodium heparin crude product; adding parenzyme into a filtering liquid, keeping the temperature to carry out the zymolysis and filtering to obtain an amino acid solution. The method for extracting sodium heparin and co-producing amino acid co-produces the amino acid solution on the basis of extracting the sodium heparin, thereby improving the economic effect, reducing the waste liquid and lightening the environment pollution.
Description
Technical field
The present invention relates to a kind of method of from chitterlings, extracting heparin sodium, particularly a kind of method of extracting sodium heparin and co-producing amino acid.
Background technology
Extract heparin sodium and mainly contain salt solution and enzymolysis process from chitterlings: the enzymolysis process yield is higher, and average 1800 chitterlings go out 1 kilogram of heparin sodium crude (tire is 100), but can produce a large amount of waste liquids, mainly contain polypeptide and protein in the waste liquid.Above-mentioned liquid waste disposal is handled very complicated, and environment is polluted.
Summary of the invention
The object of the present invention is to provide a kind of method of extracting sodium heparin and co-producing amino acid, utilize the waste liquid that extracts heparin sodium to prepare amino acid, reduce the generation of waste liquid, the pollution that alleviates environment.
The present invention realizes by following measure:
The invention discloses a kind of method of extracting sodium heparin and co-producing amino acid, it is characterized in that adopting following steps:
A) will scrape the mucous membrane of small intestine suction enzymatic vessel that makes, adding accounts for the refined salt that intestinal mucosa weight is 1%-1.8%, transfers the solution pH value to 9.0-9.2;
Add the fresh pig pancreas when b) solution in the step (a) being warmed to 40 ℃-45 ℃, the add-on of fresh pig pancreas is the 0.4-0.8% of solution weight, continues to be warmed to 50 ℃-55 ℃, insulation enzymolysis 4-5 hour, continue to be warmed to 80 ℃-85 ℃ then, be incubated 10-20 minute after-filtration;
C) filtrate in the step (b) adds the resin that pre-treatment is good, and the resin add-on is the 4-5% of filtrate weight, and whip attachment 6 hours is extracted resin, and the resin of collecting is washed till neutrality in clear water; With 17--18% (weight) strong brine that is equivalent to 1.2 times of weight resins to resin elution 2 times, head times 6 hours, secondary 2 hours is collected this elutriant hybrid filtering of twice, removes particulate contamination; In the elutriant after filtration, adding and elutriant stir a moment with 95% (weight) alcohol of volume, static state deposition, and precipitation is dewatered, and drying gets the heparin crude product;
D) filtrate after the resin absorption in the step (c), transfer pH value to 7-8, add trypsinase when being warmed to 40 ℃-45 ℃, tryptic add-on is the 0.3%-0.4% of filtrate weight, continues to be warmed to 55 ℃-60 ℃, insulation enzymolysis 4-5 hour, continue to be warmed to 80 ℃-85 ℃ then, be incubated 10-20 minute after-filtration, the gac of adding 4% adsorbs in the filtrate, gets amino acid solution.
The method of extraction sodium heparin and co-producing amino acid of the present invention is being extracted on the basis of heparin sodium, coproduction amino acid solution, improved economic benefit, reduce the generation of waste liquid, the pollution that alleviates environment.
Embodiment
One, raw material D254 resin, fresh intestinal mucosa, sodium-chlor (refined salt), alcohol (technical grade, content 95% weight), alkali lye (technical grade, content 30--40%).
Two, operation
1. will scrape the 1000 kilograms of suction enzymatic vessels of mucous membrane of small intestine that make, the refined salt that adds 15 kilograms, transfer the solution pH value to 9.0-9.2, add 6 kilograms of fresh pig pancreases when being warmed to 40 ℃-45 ℃, continue to be warmed to 50 ℃-55 ℃, insulation enzymolysis 5 hours continues to be warmed to 80 ℃-85 ℃ then, is incubated 15 minutes after-filtration; Filtrate adds 45 kilograms of D254 resins that pre-treatment is good, and whip attachment 6 hours is extracted resin, and the resin of collecting is washed till neutrality in clear water; With 54 kilograms of 17--18% (weight) strong brine to resin elution 2 times, head times 6 hours, secondary 2 hours is collected this elutriant hybrid filtering of twice, removes particulate contamination; In the elutriant after filtration, adding and elutriant stir a moment with 95% (weight) alcohol of volume, static state deposition, and precipitation is dewatered, and drying gets heparin crude product 550 grams, and quality meets the heparin sodium standard after testing, tires more than 100.
2. with 900 kilograms of the filtrates after the above-mentioned resin absorption, transfer pH value to 7-8, add 3.2 kilograms of trypsinase when being warmed to 40 ℃-45 ℃, continue to be warmed to 60 ℃, insulation enzymolysis 4 hours continues to be warmed to 85 ℃ then, is incubated 150 minutes after-filtration, the gac of adding 4% adsorbs in the filtrate, gets 850 kilograms of amino acid solutions.After testing, aminoacids content is 5%.Above-mentioned amino acid solution can be used for plant nutrition liquid through being concentrated into 10%, or as the mother liquor of other plant nutrition liquid.
Claims (1)
1. method of extracting sodium heparin and co-producing amino acid is characterized in that adopting following steps:
A) will scrape the mucous membrane of small intestine suction enzymatic vessel that makes, adding accounts for the refined salt that intestinal mucosa weight is 1%-1.8%, transfers the pH value of solution value to 9.0-9.2;
Add the fresh pig pancreas when b) solution in the step (a) being warmed to 40 ℃-45 ℃, the add-on of fresh pig pancreas is the 0.4-0.8% of solution weight, continues to be warmed to 50 ℃-55 ℃, insulation enzymolysis 4-5 hour, continue to be warmed to 80 ℃-85 ℃ then, be incubated 10-20 minute after-filtration;
C) filtrate in the step (b) adds the good resin of pre-treatment, and the resin add-on is the 4-5% of filtrate weight, and whip attachment 6 hours is extracted resin, and the resin of collecting is washed till neutrality in clear water; With 17--18% (weight) strong brine that is equivalent to 1.2 times of weight resins to resin elution 2 times, head times 6 hours, secondary 2 hours is collected this elutriant hybrid filtering of twice, removes particulate contamination; In the elutriant after filtration, adding and elutriant stir a moment with 95% (weight) alcohol of volume, static state deposition, and precipitation is dewatered, and drying gets the heparin crude product;
D) filtrate after the resin absorption in the step (c), adjust pH is to 7-8, add trypsinase when being warmed to 40 ℃-45 ℃, tryptic add-on is the 0.3%-0.4% of filtrate weight, continues to be warmed to 55 ℃-60 ℃, insulation enzymolysis 4-5 hour, continue to be warmed to 80 ℃-85 ℃ then, be incubated 10-20 minute after-filtration, the gac of adding 4% adsorbs in the filtrate, gets amino acid solution;
Described resin is the D254 resin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100189690A CN101659714B (en) | 2009-09-22 | 2009-09-22 | Method for extracting sodium heparin and co-producing amino acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100189690A CN101659714B (en) | 2009-09-22 | 2009-09-22 | Method for extracting sodium heparin and co-producing amino acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101659714A CN101659714A (en) | 2010-03-03 |
| CN101659714B true CN101659714B (en) | 2011-07-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2009100189690A Expired - Fee Related CN101659714B (en) | 2009-09-22 | 2009-09-22 | Method for extracting sodium heparin and co-producing amino acid |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286592B (en) * | 2011-09-20 | 2014-03-26 | 上海杰隆生物制品股份有限公司 | Preparation method for pork lung protein peptide |
| CN102796792A (en) * | 2012-08-30 | 2012-11-28 | 广元市海鹏生物科技有限公司 | Preparation method for chitterlings membrane protein peptide zinc chelate preparation |
| CN102924626A (en) * | 2012-09-19 | 2013-02-13 | 杭州龙扬生物科技有限公司 | Process for extracting heparin sodium efficiently from small intestines of pigs |
| CN102911291A (en) * | 2012-11-22 | 2013-02-06 | 北大荒丰缘集团有限公司 | Device suitable for automatic production of extraction industry of casing and sodium heparin |
| CN104098717A (en) * | 2014-07-21 | 2014-10-15 | 南通恒阳生物科技有限公司 | Method for extracting heparin sodium |
| CN105265754A (en) * | 2014-07-25 | 2016-01-27 | 叶青理 | Method of extracting proteins and amino acids from enzymolysis waste liquid of porcine small intestinal mucous membrane |
| CN104498553B (en) * | 2014-12-20 | 2018-06-19 | 山东绅联生物科技有限公司 | A kind of method that coarse amino acid is extracted in the heparin waste liquor of sodium from extraction |
| CN111995698A (en) * | 2020-08-07 | 2020-11-27 | 无锡海思瑞科技有限公司 | Method for simultaneously extracting heparin sodium and intestinal membrane protein peptide based on enzymolysis method |
| CN112159486B (en) * | 2020-10-15 | 2023-05-05 | 河北常山凯库得生物技术有限公司 | Method for preparing crude heparin sodium by utilizing low salt of small intestine mucosa of pig |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1291654A (en) * | 1999-10-11 | 2001-04-18 | 刘中新 | Production process for extracting heparin sodium by activated biological enzymolysis method |
| CN1308088A (en) * | 2001-02-28 | 2001-08-15 | 灌云县金瑞生物制品有限公司 | Zymolysis process of producing heparin sodium |
| CN1566162A (en) * | 2003-07-07 | 2005-01-19 | 张国良 | Heparin sodium and its preparing process |
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2009
- 2009-09-22 CN CN2009100189690A patent/CN101659714B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1291654A (en) * | 1999-10-11 | 2001-04-18 | 刘中新 | Production process for extracting heparin sodium by activated biological enzymolysis method |
| CN1308088A (en) * | 2001-02-28 | 2001-08-15 | 灌云县金瑞生物制品有限公司 | Zymolysis process of producing heparin sodium |
| CN1566162A (en) * | 2003-07-07 | 2005-01-19 | 张国良 | Heparin sodium and its preparing process |
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| CN101659714A (en) | 2010-03-03 |
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