CN106579423A - Comprehensive extraction method for various effective components in purple sweet potatoes - Google Patents
Comprehensive extraction method for various effective components in purple sweet potatoes Download PDFInfo
- Publication number
- CN106579423A CN106579423A CN201611093012.9A CN201611093012A CN106579423A CN 106579423 A CN106579423 A CN 106579423A CN 201611093012 A CN201611093012 A CN 201611093012A CN 106579423 A CN106579423 A CN 106579423A
- Authority
- CN
- China
- Prior art keywords
- purple sweet
- steudnerae henryanae
- rhizoma steudnerae
- sweet potatoes
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 244000017020 Ipomoea batatas Species 0.000 title claims abstract description 14
- 235000002678 Ipomoea batatas Nutrition 0.000 title claims abstract description 14
- 238000000605 extraction Methods 0.000 title claims abstract description 10
- 238000001914 filtration Methods 0.000 claims abstract description 25
- 235000013325 dietary fiber Nutrition 0.000 claims abstract description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 15
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000000706 filtrate Substances 0.000 claims abstract description 14
- 239000004744 fabric Substances 0.000 claims abstract description 13
- 229920002472 Starch Polymers 0.000 claims abstract description 12
- 235000019698 starch Nutrition 0.000 claims abstract description 12
- 239000008107 starch Substances 0.000 claims abstract description 12
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 11
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 11
- 239000006228 supernatant Substances 0.000 claims abstract description 11
- 239000000843 powder Substances 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 6
- 238000004140 cleaning Methods 0.000 claims abstract description 4
- 239000000203 mixture Substances 0.000 claims abstract description 4
- 239000002245 particle Substances 0.000 claims abstract description 4
- 238000001556 precipitation Methods 0.000 claims abstract description 4
- 239000002244 precipitate Substances 0.000 claims description 12
- 229930014669 anthocyanidin Natural products 0.000 claims description 11
- 150000001452 anthocyanidin derivatives Chemical class 0.000 claims description 11
- 235000008758 anthocyanidins Nutrition 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 4
- 239000012467 final product Substances 0.000 claims description 4
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 235000011837 pasties Nutrition 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims description 2
- 238000010025 steaming Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 12
- 239000002994 raw material Substances 0.000 abstract description 3
- 238000011437 continuous method Methods 0.000 abstract description 2
- 239000002002 slurry Substances 0.000 abstract description 2
- 239000000243 solution Substances 0.000 abstract 4
- 229930002877 anthocyanin Natural products 0.000 abstract 2
- 235000010208 anthocyanin Nutrition 0.000 abstract 2
- 239000004410 anthocyanin Substances 0.000 abstract 2
- 150000004636 anthocyanins Chemical class 0.000 abstract 2
- 235000019441 ethanol Nutrition 0.000 abstract 2
- 235000019750 Crude protein Nutrition 0.000 abstract 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 abstract 1
- 229910052739 hydrogen Inorganic materials 0.000 abstract 1
- 239000001257 hydrogen Substances 0.000 abstract 1
- 239000011259 mixed solution Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 description 4
- 239000000835 fiber Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920001592 potato starch Polymers 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
- C08B30/04—Extraction or purification
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention relates to a method which takes purple sweet potatoes as raw materials and is used for comprehensively extracting starch, anthocyanin, soluble protein, soluble dietary fibers and insolubility dietary fibers. The method mainly includes the process steps: pretreatment; acid-extraction; isoelectric precipitation; alcohol precipitation; concentration and the like. The method includes the steps: selecting fresh purple sweet potatoes, cleaning the purple sweet potatoes, slicing the cleaned purple sweet potatoes, and crushing slices to obtain purple sweet potato dry powder with 60-mesh particle size or purple sweet potato slurry; performing ultrasonic-assisted extraction for 1 hour by the aid of citric acid solution with pH (potential of hydrogen) of 2.0, and filtering mixture by the aid of filter cloth to obtain insoluble dietary fibers and muddy filter solution; centrifuging the muddy filter solution to obtain crude starch and liquid supernatant, concentrating the liquid supernatant, adjusting the pH to be 4, standing, and centrifuging the mixture to obtain crude protein; adding the liquid supernatant into 95% ethyl alcohol, standing, filtering mixed solution to obtain the soluble dietary fibers, and concentrating filtrate in a vacuum manner to obtain anthocyanin concentrated solution. According to the method, various effective components are extracted once by the aid of a simple, convenient and continuous method, a process is free from pollution, the obtained protein is non-degenerated, and the method has important significance for comprehensive utilization of the purple sweet potatoes.
Description
Technical field
The present invention relates to a kind of is raw material using Rhizoma Steudnerae Henryanae, through it is high-valued, clean processing step, realize that Rhizoma Steudnerae Henryanae is efficiently sharp
With acquisition multiple product simultaneously.
Background technology
It is in atropurpureuss, Sweetpotato of the meat in purple to darkviolet that Rhizoma Steudnerae Henryanae is a class potato skin.In recent years, because which contains ten
Divide the multiple nutritional components such as abundant anthocyanidin, selenium, polysaccharide, vegetable protein, vitamin and mineral, enjoy people blue or green
Look at.
In fact, Rhizoma Steudnerae Henryanae is precious from head to foot.Research shows that Jing often to eat Rhizoma Dioscoreae esculentae can be remarkably reinforced human body immunity, right
Digestive tract, cardiovascular and cerebrovascular disease have preferable preventive effect, and its anti-cancer and cancer-preventing ability is occupied first of various foods, thus have higher
Edible and medical value.The extracting and developing and the applied research at aspects such as health cares of Rhizoma Steudnerae Henryanae functional component are carried out, it is high to which
Value is using very necessary.
At present, to the research of Rhizoma Steudnerae Henryanae mainly for its starch and anthocyanidin, and so far there is not yet to its starch, anthocyanidin,
The report of the comprehensive extracting method of soluble protein, water soluble dietary fiber and insoluble dietary fiber.
Currently invention addresses the higher value application of Rhizoma Steudnerae Henryanae resource, according to the content and property feature of all kinds of materials, adopting will
The comprehensive extraction work that starch, anthocyanidin, soluble protein, soluble fiber and insoluble fibers are extracted in Rhizoma Steudnerae Henryanae
Skill, so as to obtain 5 kinds of product effective ingredient (can be used as food enrichment, functional food functional component) etc., thus can be with
Abundant high-value-use Rhizoma Steudnerae Henryanae resource;On products and marketing of the present invention, existing Rhizoma Steudnerae Henryanae product is compared, with obvious technology and
Quality-advantage.
The content of the invention
It is an object of the invention to provide with Rhizoma Steudnerae Henryanae as raw material, combined extracting starch, anthocyanidin, soluble protein, can
The method of soluble dietary fiber and insoluble dietary fiber.
To achieve these goals, combined extraction technology process of the invention is as follows:
(1) pretreatment:Fresh Rhizoma Steudnerae Henryanae is chosen, is cut into slices after cleaning up, crushed after drying at 40 DEG C -55 DEG C, make Rhizoma Steudnerae Henryanae
Powder Particle Size is controlled below 60 mesh.Or directly crush in the pasty state after fresh Rhizoma Steudnerae Henryanae is cleaned, disposably plus 1~2 times of light violet potato weights
With 60 mesh filter-cloth filterings after the distilled water of amount, filtering residue is crushed again up to Rhizoma Steudnerae Henryanae granularity completely below 60 mesh, obtains Rhizoma Steudnerae Henryanae serosity.
(2) acid is carried:A certain amount of purple sweet potato powder is taken, the solid-liquid ratio for comparing 1: 8~1: 10 with extracting solution weight according to purple sweet potato powder enters
Row ultrasound assisted extraction, extracting solution pH2.0, extraction time are 20~60min, obtain filtering residue and muddy filter with 100 mesh filter-cloth filterings
Liquid.Filtrate is centrifuged under 2000r/min, precipitate is dried at 40 DEG C -55 DEG C, that is, is obtained Crude starch;Or take Rhizoma Steudnerae Henryanae slurry
Liquid, plus appropriate citric acid control mixture pH for 2.0,20~60min of ultrasound assisted extraction time, with 100 mesh filter-cloth filterings, general
Filtrate is centrifuged under 2000r/min, precipitate is dried at 40 DEG C -55 DEG C, that is, obtains Crude starch.
(3) isoelectric precipitation:Gained filtering residue in (2) is dried at 70-85 DEG C and is smashed, that is, obtain insoluble meals fine
Dimension;Supernatant after centrifugation in (2) is concentrated in vacuo to into the 1/5~1/6 of original volume at 50 DEG C, then adjusts pH value of solution 4, stood
Overnight.The precipitate for obtaining is centrifuged under 4000r/min and is thick protein.
(4) precipitate with ethanol:The supernatant obtained after centrifugation in (3) is added into 95% ethanol of 4 times of volumes, is stood overnight, 100 mesh
Filter-cloth filtering, filter residue and drying obtain final product water soluble dietary fiber.
(5) concentrate:Filtrate in (4) is concentrated in vacuo to into the 1/5~1/6 of original volume at 50 DEG C, that is, obtains anthocyanidin dense
Contracting liquid.
Compared with prior art, present invention process scheme passes through simplicity, a continuous method so that purple sweet potato starch, flower
Blue or green element, protein, water soluble dietary fiber and insoluble dietary fiber are disposably efficiently extracted, and do not produce any adding
Work waste, the course of processing is without any pollution, and the protein for obtaining is non denatured, therefore, the comprehensive utilization to Rhizoma Dioscoreae esculentae
It is significant.
Description of the drawings
Fig. 1 is the integrated extraction technique flow chart of plurality of active ingredients in Rhizoma Steudnerae Henryanae.
Specific embodiment
Below by example, the present invention is further elaborated:
Example 1:The fresh Rhizoma Steudnerae Henryanae of 3.3kg is chosen, is cut into slices after cleaning up, crushed after drying at 40 DEG C -55 DEG C, make Rhizoma Steudnerae Henryanae
Powder Particle Size control obtains Rhizoma Steudnerae Henryanae dry powder 1.0kg below 60 mesh.Plus the citric acid solution ultrasound assisted extraction 1h of 8kg pH2.0, with
100 mesh filter-cloth filterings obtain filtering residue and muddy filtrate.Filtering residue is dried at 60 DEG C, is crushed, obtain insoluble dietary fiber 240g;Will
Muddy filtrate is centrifuged under 2000r/min, by precipitate at 40 DEG C -55 DEG C drying Crude starch 470g;Supernatant is existed
The 1/5 of original volume is concentrated in vacuo at 50 DEG C, pH value of solution 4 is adjusted, is stood overnight.Be centrifuged under 4000r/min precipitate (i.e.
Thick protein) 9g, supernatant add 4 times of volumes 95% ethanol, stand overnight, 100 mesh filter-cloth filterings, filter residue and drying is obtained final product can
Soluble dietary fiber 25g, filtrate are concentrated in vacuo to the 1/6 of original volume at 50 DEG C, that is, obtain anthocyanidin concentrated solution 1.39kg and (contain
1.88g anthocyanidin).
Example 2:Directly crush in the pasty state, with 60 mesh after disposable plus 5.7kg distilled water after fresh Rhizoma Steudnerae Henryanae 3.3kg is cleaned
Filter-cloth filtering, filtering residue is crushed again until Rhizoma Steudnerae Henryanae granularity obtains Rhizoma Steudnerae Henryanae serosity 9kg completely below 60 mesh.Adding citric acid adjusts mixed liquor
PH2.0, ultrasound assisted extraction 1h, obtain filtering residue and muddy filtrate with 100 mesh filter-cloth filterings.Filtering residue is dried at 60 DEG C, is crushed,
Obtain insoluble dietary fiber 240g;Muddy filtrate is centrifuged under 2000r/min, precipitate is dried at 40 DEG C -55 DEG C is
Obtain Crude starch 470g;Supernatant is concentrated in vacuo to into the 1/5 of original volume at 50 DEG C, pH value of solution 4 is adjusted, is stood overnight.
Precipitate i.e. thick protein 9g is centrifuged to obtain under 4000r/min, supernatant adds 95% ethanol of 4 times of volumes, stands overnight, 100
Mesh filter-cloth filtering, filter residue and drying obtain final product water soluble dietary fiber 25g, and filtrate is concentrated in vacuo to the 1/6 of original volume at 50 DEG C, i.e.,
Obtain anthocyanidin concentrated solution 1.39kg (anthocyanidin containing 2.67g).
Claims (1)
1. in a kind of Rhizoma Steudnerae Henryanae plurality of active ingredients combined extraction method, comprise the steps:
(1) pretreatment:Fresh Rhizoma Steudnerae Henryanae is chosen, is cut into slices after cleaning up, crushed after drying at 40~55 DEG C, make Rhizoma Steudnerae Henryanae Powder Particle Size
Control is below 60 mesh;Or directly crushing in the pasty state, disposably plus 1~steaming of 3 times of light violet potato weight after fresh Rhizoma Steudnerae Henryanae is cleaned
With 60 mesh filter-cloth filterings after distilled water, filtering residue is crushed again up to Rhizoma Steudnerae Henryanae granularity completely below 60 mesh, obtains Rhizoma Steudnerae Henryanae serosity;
(2) acid is carried:A certain amount of purple sweet potato powder is taken, the solid-liquid ratio for comparing 1: 8~1: 10 with extracting solution weight according to purple sweet potato powder is surpassed
Sound assisted extraction, extracting solution pH2.0, extraction time are 20~60min, obtain filtering residue and muddy filtrate with 100 mesh filter-cloth filterings;Will
Filtrate is centrifuged under 2000r/min, precipitate is dried at 40~55 DEG C, that is, obtains Crude starch;Or Rhizoma Steudnerae Henryanae serosity is taken, plus it is suitable
It is 2.0,20~60min of ultrasound assisted extraction time that amount citric acid controls mixture pH, with 100 mesh filter-cloth filterings, filtrate is existed
It is centrifuged under 2000r/min, precipitate is dried at 40~55 DEG C, that is, obtains Crude starch;
(3) isoelectric precipitation:Gained filtering residue in (2) is dried at 70~85 DEG C and is smashed, that is, obtain insoluble dietary fiber;Will
(2) supernatant in after centrifugation is concentrated in vacuo to the 1/5~1/8 of original volume at 50 DEG C, then adjusts pH value of solution 4, stands overnight,
The precipitate for obtaining is centrifuged under 4000r/min and is thick protein;
(4) precipitate with ethanol:The supernatant obtained after centrifugation in (3) is added into 95% ethanol of 4 times of volumes, is stood overnight, 100 mesh filter clothes
Filter, filter residue and drying obtains final product water soluble dietary fiber;
(5) concentrate:Filtrate in (4) is concentrated in vacuo to into the 1/5~1/8 of original volume at 50 DEG C, that is, obtains anthocyanidin concentrated solution.
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CN201611093012.9A CN106579423A (en) | 2016-11-24 | 2016-11-24 | Comprehensive extraction method for various effective components in purple sweet potatoes |
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CN201611093012.9A CN106579423A (en) | 2016-11-24 | 2016-11-24 | Comprehensive extraction method for various effective components in purple sweet potatoes |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107811081A (en) * | 2017-10-20 | 2018-03-20 | 刘青亚 | A kind of production method of peppermint tea chewable tablet |
CN108813640A (en) * | 2018-05-18 | 2018-11-16 | 西南林业大学 | A method of dietary fiber is extracted by raw material of purple sweet potato |
CN110651894A (en) * | 2019-10-31 | 2020-01-07 | 上海耐威克宠物用品有限公司 | Pet health product for improving immunity and preparation method thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102206426A (en) * | 2011-03-23 | 2011-10-05 | 武汉普赛特膜技术循环利用有限公司 | Comprehensive utilization of pigment of purple sweet potato in purple sweet potato and method for comprehensively utilizing component thereof |
CN104031019A (en) * | 2014-07-04 | 2014-09-10 | 中国科学院成都生物研究所 | Rapid extracting method of purple potato anthocyanins |
CN104130333A (en) * | 2014-07-21 | 2014-11-05 | 天津商业大学 | Method for synchronous production of anthocyanidin, starch, cellulose and protein by using purple sweet potato |
CN105175382A (en) * | 2015-11-06 | 2015-12-23 | 四川光友薯业有限公司 | Comprehensive processing and utilization method of purple sweet potatoes |
CN105433393A (en) * | 2015-11-30 | 2016-03-30 | 广西师范大学 | Method for preparing dietary fiber from purple sweet potato residues used as raw materials |
-
2016
- 2016-11-24 CN CN201611093012.9A patent/CN106579423A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102206426A (en) * | 2011-03-23 | 2011-10-05 | 武汉普赛特膜技术循环利用有限公司 | Comprehensive utilization of pigment of purple sweet potato in purple sweet potato and method for comprehensively utilizing component thereof |
CN104031019A (en) * | 2014-07-04 | 2014-09-10 | 中国科学院成都生物研究所 | Rapid extracting method of purple potato anthocyanins |
CN104130333A (en) * | 2014-07-21 | 2014-11-05 | 天津商业大学 | Method for synchronous production of anthocyanidin, starch, cellulose and protein by using purple sweet potato |
CN105175382A (en) * | 2015-11-06 | 2015-12-23 | 四川光友薯业有限公司 | Comprehensive processing and utilization method of purple sweet potatoes |
CN105433393A (en) * | 2015-11-30 | 2016-03-30 | 广西师范大学 | Method for preparing dietary fiber from purple sweet potato residues used as raw materials |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107811081A (en) * | 2017-10-20 | 2018-03-20 | 刘青亚 | A kind of production method of peppermint tea chewable tablet |
CN108813640A (en) * | 2018-05-18 | 2018-11-16 | 西南林业大学 | A method of dietary fiber is extracted by raw material of purple sweet potato |
CN110651894A (en) * | 2019-10-31 | 2020-01-07 | 上海耐威克宠物用品有限公司 | Pet health product for improving immunity and preparation method thereof |
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Application publication date: 20170426 |