CN114592018A - Production method for preparing high maltose syrup and dietary fiber by using corn starch processing waste residues - Google Patents
Production method for preparing high maltose syrup and dietary fiber by using corn starch processing waste residues Download PDFInfo
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- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 title claims abstract description 77
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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Abstract
The invention discloses a production method for preparing high maltose syrup and dietary fiber by using corn starch processing waste residues, belonging to the technical field of food biology. The invention utilizes the byproduct corn starch processing waste residue which is produced in large amount in the starch sugar enterprise production, the corn starch processing waste residue is properly crushed and then is added with water for uniform mixing, the filtrate and the waste residue which are obtained by filtering are converted into high maltose syrup and dietary fiber through a series of processing technologies, and can be continuously converted in large batch, thereby not only reducing the pressure of waste treatment, but also bringing economic benefit to enterprises.
Description
Technical Field
The invention relates to the technical field of food biology, in particular to a production method for preparing high maltose syrup and dietary fiber by using corn starch processing waste residues.
Background
At present, the three main raw materials of the domestic starch sugar industry are corn, wheat and potato, wherein the corn has high yield and low production cost, so the starch sugar is generally prepared by taking corn starch as the raw material in the current sugar industrial production. However, starch sugar enterprises also accompany a large amount of byproducts and leftovers when producing main products of corn starch, corn starch processing waste residues occupy a large part of the corn starch processing waste residues, and the corn starch processing waste residues are difficult to dry due to high humidity, easy to heat and rot due to stacking, and cause great pollution to the environment due to improper treatment or random discarding.
The maltose syrup is prepared by processing starch serving as a raw material through a series of processes, and can be divided into three types according to the content of maltose in the maltose syrup: ordinary maltose syrup, high maltose syrup and ultrahigh maltose syrup, wherein the ultrahigh maltose syrup is delicate and complicated in process and high in production cost, and the ordinary maltose syrup cannot meet the requirement of some products on high maltose purity. The high maltose syrup has low and mild sweetness, strong palatability and good taste, and is not easy to generate Maillard reaction when being heated. The high maltose syrup has the advantages of low DE value, high boiling temperature, good toughness, high transparency and the like when being used for candy production, can also reduce the viscosity of the candy, improve the flavor of the product and bring obvious economic benefits to enterprises.
The dietary fiber is a general term for a plant edible part which is difficult to be digested by enzymolysis in the small intestine of a human body and is completely or partially fermented in the large intestine, and comprises substances such as cellulose, hemicellulose, pectin and the like, and is rich in vegetables, fruits, coarse grain beans and bacteria and algae food. With the progress of the times, the dietary structure of people is more refined, and the intake of carbohydrates derived from plants is reduced, so that the intake of dietary fibers is relatively reduced, and various chronic diseases threatening the health of human beings are followed; with the intensive understanding of dietary fiber, modern people have recognized that dietary fiber has many beneficial effects on the human body such as anti-diarrhea, intestinal cancer prevention, constipation treatment, cholelithiasis treatment, blood cholesterol and triglyceride reduction, blood sugar reduction in adult diabetics, and the like.
Therefore, the research on a production method which can effectively utilize the corn starch processing waste residue to convert the corn starch processing waste residue into high maltose syrup and dietary fiber with edible value has high value.
Disclosure of Invention
The invention aims to provide a production method for preparing high maltose syrup and dietary fiber by using corn starch processing waste residues. In order to achieve the purpose, the invention adopts the following technical scheme:
(1) properly crushing the corn starch processing waste residue, adding water, mixing uniformly, stirring at 70-80 ℃ for 30-50 min, cooling to 35-40 ℃, adjusting the pH to 5.5-7.0, adding protease for treatment for 3h, heating to inactivate enzyme, and primarily filtering to obtain filtrate and waste residue A;
(2) centrifuging the filtrate to obtain dehydrated corn starch milk and centrifugate;
(3) mixing the waste residue A and the corn starch milk in the step (2), adjusting the pH value to 5.5-6.5, adding alpha-amylase, keeping the temperature at 50-65 ℃ for 40min, and heating to inactivate the enzyme for 5 min;
(4) adding saccharifying enzyme into the liquefied feed liquid, controlling the saccharifying temperature to be 50-65 ℃, controlling the saccharifying time to be 30-40 h, heating to inactivate enzyme after saccharifying is finished, and filtering to obtain maltose liquid and waste residue B;
(5) refining the maltose liquid through a series of steps of decoloring, ion exchange and concentration to obtain high maltose syrup;
(6) and uniformly mixing the waste residue B and the collected centrifugate with water, carrying out suction filtration to obtain filtrate and filter residues, carrying out alcohol precipitation treatment on the filtrate at room temperature, carrying out suction filtration again, and finally drying, crushing and sieving the two filter residues to obtain the dietary fiber.
Further preferably, the corn starch processing waste residue used in the step (1) is prepared by mixing 1: 1, adding water, mixing, and adding 0.5-1% of protease.
Further preferably, the step (2) is performed by a horizontal scraper centrifuge.
Further preferably, the concentration of the starch after size mixing in the step (3) is 20-30%, 0.2-0.5 kg/t of dry starch is added with alpha-amylase, and the liquefied DE value is controlled to be 25-40.
More preferably, the addition amount of the saccharifying enzyme in the step (4) is 3-5 kg/t dry starch.
Further preferably, 0.2% -0.5% of activated carbon is added in the step (5) to decolor for 20-30 min at 80-85 ℃, ion exchange is carried out through a cation-anion-cation-anion exchange column, and a four-effect concentrator is adopted to concentrate to 70% -80% of concentration, so that the high maltose syrup is obtained.
Further preferably, the step (6) is performed in a ratio of 1: 10, mixing with water, freezing and drying at-15 to-20 ℃, and sieving by a standard sieve of 100 meshes to obtain the dietary fiber with the water content of 5-12 percent.
The invention has the following beneficial effects:
if the corn starch processing waste residue is dried and crushed by adopting the traditional treatment method in the starch production, the part of resources cannot be utilized to the maximum extent, and the corn starch processing waste residue is a main byproduct, so that the production amount is large and the treatment is very troublesome. The method comprises the steps of crushing the corn starch processing waste residue properly, adding water, mixing uniformly, adding protease, removing proteolysis contained in the corn starch processing waste residue by enzymolysis, providing a more compatible substrate for subsequent saccharification of starch, centrifuging, primarily separating out starch milk, further saccharifying the starch milk obtained by filtering and the waste residue to prepare high maltose syrup, and effectively utilizing the residual starch in the corn starch processing waste residue; supernatant obtained by centrifugation and waste residue obtained by saccharification and starch extraction are added with water and filtered to obtain filtrate for secondary alcohol precipitation, and the waste residue is taken for drying and crushing to obtain high-quality dietary fiber.
The invention utilizes the corn starch processing waste residue which is produced in large amount in the production of starch sugar enterprises, converts the waste residue into high maltose syrup and dietary fiber through a series of processing technologies, and reuses the waste resources, thereby not only reducing the pressure of waste treatment, but also bringing economic benefits to the enterprises, having great pertinence to the production and pollution discharge of the starch sugar enterprises, and being suitable for popularization and use.
Detailed Description
The present invention is further illustrated by the following examples, but the scope of the present invention is not limited to the following examples, and the present invention can be implemented or applied by various embodiments, and all other examples obtained by a person of ordinary skill in the art without any inventive work are within the scope of the present invention.
The invention provides a production method for preparing high maltose syrup and dietary fiber by utilizing corn starch processing waste residues, which comprises the following steps:
(1) properly crushing the corn starch processing waste residue in a proportion of 1: 1, adding water, uniformly mixing, stirring at 70-80 ℃ for 30-50 min, cooling to 35-40 ℃, adjusting the pH to 5.5-7.0, adding 0.5-1% of protease for treatment for 3h, heating to inactivate enzyme, and primarily filtering to obtain filtrate and waste residue A;
(2) centrifuging the filtrate to obtain dehydrated corn starch milk and centrifugate;
(3) mixing the waste residue A and the corn starch milk in the step (2) to be pulp-mixed until the concentration is 20-30%, adjusting the pH value to 5.5-6.5, adding 0.2-0.5 kg/t of dry starch of alpha-amylase, preserving the heat for 40min at 50-65 ℃, and heating to inactivate the enzyme for 5 min;
(4) liquefying to obtain a feed liquid with a DE value of 25-40, adding 3-5 kg/t of dry starch by using saccharifying enzyme, controlling the saccharifying temperature at 50-65 ℃, saccharifying for 30-40 h, heating to inactivate enzyme after saccharifying is finished, and filtering to obtain a maltose liquid and waste residue B;
(5) adding 0.2-0.5% of activated carbon into the maltose liquid, decoloring for 20-30 min at 80-85 ℃, performing ion exchange through a cation-anion-cation-anion exchange column, and concentrating to a concentration of 70-80% by using a four-effect concentrator to obtain the high maltose syrup.
(6) Waste B and collected centrifugate were mixed at a ratio of 1: 10, filtering to obtain filtrate and filter residue, carrying out alcohol precipitation treatment on the filtrate at room temperature, carrying out suction filtration again, finally freeze-drying the two parts of filter residue at the temperature of between 15 ℃ below zero and 20 ℃ below zero, and sieving by a 100-mesh standard sieve to obtain the dietary fiber with the water content of 5-12%.
Example 1
The corn starch processing waste residue (water content 85.0%, starch content 45.0%) is appropriately crushed in a ratio of 1: 1, adding water, uniformly mixing, stirring at 70-80 ℃ for 30min, cooling to 35-40 ℃, adjusting the pH to 5.5, adding 0.5% protease for treatment for 3h, heating to inactivate enzyme, and primarily filtering to obtain filtrate and waste residue A; centrifuging the filtrate to obtain dehydrated corn starch milk and centrifugate; mixing the waste residue A and the corn starch milk, adjusting the slurry to the concentration of 20%, adjusting the pH value to 5.5, adding 0.2kg/t of dry starch of alpha-amylase, preserving the heat at 50-65 ℃ for 40min, and heating to inactivate the enzyme for 5 min; liquefying to obtain a feed liquid with a DE value of 25, adding 3kg/t of dry starch by using saccharifying enzyme, controlling the saccharifying temperature to be 50-65 ℃, saccharifying for 30 hours, heating to inactivate enzyme after saccharifying is finished, and filtering to obtain maltose liquid and waste residue B; adding 0.2% of active carbon into the maltose syrup liquid, decoloring for 20min at the temperature of 80-85 ℃, performing ion exchange through a cation-anion-cation-anion exchange column, and concentrating to the concentration of 70% by adopting a four-effect concentrator to obtain the high maltose syrup with the maltose content of 72.5%. The waste residue B and the collected centrifugate were mixed in a ratio of 1: 10, obtaining filtrate and filter residues, carrying out alcohol precipitation treatment on the filtrate at room temperature, carrying out suction filtration again, finally freeze-drying the two parts of filter residues at the temperature of between 15 ℃ below zero and 20 ℃ below zero, and sieving the filter residues by a standard sieve of 100 meshes to obtain the dietary fiber with the effective component content of 71.5%, the water content of 7.2% and the water absorption expansibility of 15.45 mL/g.
Example 2
The corn starch processing waste residue (water content 85.6%, starch content 45.3%) is appropriately crushed in a ratio of 1: 1, adding water, uniformly mixing, stirring at 70-80 ℃ for 35min, cooling to 35-40 ℃, adjusting the pH to 5.8, adding 0.5% protease for treatment for 3h, heating to inactivate enzyme, and primarily filtering to obtain filtrate and waste residue A; centrifuging the filtrate to obtain dehydrated corn starch milk and centrifugate; mixing the waste residue A and the corn starch milk, adjusting the slurry to the concentration of 20%, adjusting the pH value to 6.0, adding 0.2kg/t of alpha-amylase into dry starch, keeping the temperature at 50-65 ℃ for 40min, and heating to inactivate the enzyme for 5 min; liquefying to obtain feed liquid with a DE value of 27, adding 3kg/t of dry starch by using saccharifying enzyme, controlling the saccharifying temperature to be 50-65 ℃, saccharifying for 30 hours, heating to inactivate enzyme after saccharifying is finished, and filtering to obtain maltose liquid and waste residue B; adding 0.2% of active carbon into the maltose liquid, decoloring for 20min at the temperature of 80-85 ℃, carrying out ion exchange by using a cation-anion-cation-anion exchange column, and concentrating to the concentration of 70% by using a four-effect concentrator to obtain the high maltose syrup with the maltose content of 73.4%. The waste residue B and the collected centrifugate were mixed in a ratio of 1: 10, obtaining filtrate and filter residues, carrying out alcohol precipitation treatment on the filtrate at room temperature, carrying out suction filtration again, finally freeze-drying the two parts of filter residues at the temperature of between 15 ℃ below zero and 20 ℃ below zero, and sieving the filter residues by a standard sieve of 100 meshes to obtain the dietary fiber with the effective component content of 71.8 percent, the water content of 6.8 percent and the water absorption expansibility of 15.8 mL/g.
Example 3
Corn starch processing waste residue (water content 85.8%, starch content 46.7%) is appropriately crushed in a ratio of 1: 1, adding water, uniformly mixing, stirring at 70-80 ℃ for 35min, cooling to 35-40 ℃, adjusting the pH to 6.0, adding 0.8% of protease, treating for 3h, heating to inactivate enzyme, and primarily filtering to obtain filtrate and waste residue A; centrifuging the filtrate to obtain dehydrated corn starch milk and centrifugate; mixing the waste residue A and corn starch milk, adjusting the slurry to the concentration of 25%, adjusting the pH value to 6.0, adding 0.3kg/t of dry starch of alpha-amylase, preserving the heat at 50-65 ℃ for 40min, and heating to inactivate the enzyme for 5 min; liquefying to obtain a feed liquid with a DE value of 28, adding 3kg/t of dry starch by using saccharifying enzyme, controlling the saccharifying temperature to be 50-65 ℃, saccharifying for 35 hours, heating to inactivate enzyme after saccharifying is finished, and filtering to obtain maltose liquid and waste residue B; adding 0.3% of active carbon into the maltose syrup liquid, decoloring for 25min at the temperature of 80-85 ℃, performing ion exchange through a cation-anion-cation-anion exchange column, and concentrating to the concentration of 75% by adopting a four-effect concentrator to obtain high maltose syrup with the maltose content of 73.8%. The waste residue B and the collected centrifugate were mixed in a ratio of 1: 10, obtaining filtrate and filter residues, carrying out alcohol precipitation treatment on the filtrate at room temperature, carrying out suction filtration again, finally freeze-drying the two parts of filter residues at the temperature of between 15 ℃ below zero and 20 ℃ below zero, and sieving the filter residues by a standard sieve of 100 meshes to obtain the dietary fiber with the effective component content of 72.9%, the water content of 8.2% and the water absorption expansibility of 16.53 mL/g.
Example 4
Corn starch processing waste residue (water content 85.9%, starch content 47.4%) is appropriately crushed in a ratio of 1: 1, adding water, uniformly mixing, stirring at 70-80 ℃ for 40min, cooling to 35-40 ℃, adjusting the pH to 6.0, adding 0.8% of protease, treating for 3h, heating to inactivate enzyme, and primarily filtering to obtain filtrate and waste residue A; centrifuging the filtrate to obtain dehydrated corn starch milk and centrifugate; mixing the waste residue A and corn starch milk, adjusting the slurry to the concentration of 25%, adjusting the pH value to 6.0, adding 0.3kg/t of dry starch of alpha-amylase, preserving the heat at 50-65 ℃ for 40min, and heating to inactivate the enzyme for 5 min; liquefying to obtain a feed liquid with a DE value of 28, adding 4kg/t of dry starch by using saccharifying enzyme, controlling the saccharifying temperature to be 50-65 ℃, saccharifying for 35 hours, heating to inactivate enzyme after saccharifying is finished, and filtering to obtain maltose liquid and waste residue B; adding 0.3% of activated carbon into the maltose syrup liquid, decoloring for 25min at the temperature of 80-85 ℃, performing ion exchange through a cation-anion-cation-anion exchange column, and concentrating to the concentration of 75% by adopting a four-effect concentrator to obtain high maltose syrup with the maltose content of 74.7%. The waste residue B and the collected centrifugate were mixed in a ratio of 1: 10, filtering to obtain filtrate and filter residues, precipitating the filtrate with ethanol at room temperature, filtering again, freeze-drying the two parts of filter residues at the temperature of between 15 ℃ below zero and 20 ℃ below zero, and sieving the filter residues through a 100-mesh standard sieve to obtain the dietary fiber with the effective component content of 73.21%, the water content of 8.8% and the water swelling property of 17.32 mL/g.
Example 5
The corn starch processing waste residue (water content 86.3%, starch content 48.2%) is appropriately crushed in a ratio of 1: 1, adding water, uniformly mixing, stirring at 70-80 ℃ for 45min, cooling to 35-40 ℃, adjusting the pH to 6.5, adding 0.8% of protease for treatment for 3h, heating to inactivate enzyme, and primarily filtering to obtain filtrate and waste residue A; centrifuging the filtrate to obtain dehydrated corn starch milk and centrifugate; mixing the waste residue A and corn starch milk, adjusting the slurry to 28% of concentration, adjusting the pH value to 6.0, adding 0.4kg/t of dry starch of alpha-amylase, keeping the temperature at 50-65 ℃ for 40min, and heating to inactivate the enzyme for 5 min; liquefying to obtain a feed liquid with a DE value of 30, adding 4kg/t of dry starch by using saccharifying enzyme, controlling the saccharifying temperature to be 50-65 ℃, saccharifying for 38 hours, heating to inactivate enzyme after saccharifying is finished, and filtering to obtain maltose liquid and waste residue B; adding 0.4% of active carbon into the maltose syrup liquid, decoloring for 25min at the temperature of 80-85 ℃, performing ion exchange through a cation-anion-cation-anion exchange column, and concentrating to the concentration of 75% by adopting a four-effect concentrator to obtain the high maltose syrup with the maltose content of 76.3%. Waste B and collected centrifugate were mixed at a ratio of 1: 10, obtaining filtrate and filter residue by suction filtration, carrying out suction filtration again after the filtrate is subjected to alcohol precipitation treatment at room temperature, finally freeze-drying the two parts of filter residue at the temperature of between 15 ℃ below zero and 20 ℃ below zero, and sieving the filter residue by a standard sieve of 100 meshes to obtain the dietary fiber with the effective component content of 74.78%, the water content of 9.3% and the water absorption expansibility of 18.43 mL/g.
Example 6
The corn starch processing waste residue (water content 86.8%, starch content 48.9%) is appropriately crushed in a ratio of 1: 1, adding water, uniformly mixing, stirring at 70-80 ℃ for 50min, cooling to 35-40 ℃, adjusting the pH to 7.0, adding 1% of protease for treatment for 3h, heating to inactivate enzyme, and primarily filtering to obtain filtrate and waste residue A; centrifuging the filtrate to obtain dehydrated corn starch milk and centrifugate; mixing the waste residue A and the corn starch milk, adjusting the slurry to the concentration of 30%, adjusting the pH value to 6.5, adding 0.5kg/t of alpha-amylase into dry starch, keeping the temperature at 50-65 ℃ for 40min, and heating to inactivate the enzyme for 5 min; liquefying to obtain feed liquid with a DE value of 35, adding 5kg/t of dry starch by using saccharifying enzyme, controlling the saccharifying temperature to be 50-65 ℃, saccharifying for 40 hours, heating to inactivate enzyme after saccharifying is finished, and filtering to obtain maltose liquid and waste residue B; adding 0.5% of active carbon into the maltose syrup liquid, decoloring for 30min at 80-85 ℃, performing ion exchange through a cation-anion-cation-anion exchange column, and concentrating to 80% by adopting a four-effect concentrator to obtain the high maltose syrup with the maltose content of 77.9%. The waste residue B and the collected centrifugate were mixed in a ratio of 1: 10, mixing with water, filtering to obtain filtrate and filter residue, precipitating the filtrate with ethanol at room temperature, filtering again, freeze-drying the two filter residues at-15-20 ℃, and sieving with a 100-mesh standard sieve to obtain the dietary fiber with the effective component content of 75.21%, the water content of 9.8% and the water swelling property of 18.32 mL/g.
Example 7
Corn starch processing waste residue (water content 87.2%, starch content 49.1%) is appropriately crushed in a ratio of 1: 1, adding water, uniformly mixing, stirring at 70-80 ℃ for 50min, cooling to 35-40 ℃, adjusting the pH to 7.0, adding 1% of protease for treatment for 3h, heating to inactivate enzyme, and primarily filtering to obtain filtrate and waste residue A; centrifuging the filtrate to obtain dehydrated corn starch milk and centrifugate; mixing the waste residue A and the corn starch milk, adjusting the slurry to the concentration of 30%, adjusting the pH value to 6.5, adding 0.5kg/t of alpha-amylase into dry starch, keeping the temperature at 50-65 ℃ for 40min, and heating to inactivate the enzyme for 5 min; liquefying to obtain feed liquid with a DE value of 35, adding 5kg/t of dry starch by using saccharifying enzyme, controlling the saccharifying temperature to be 50-65 ℃, saccharifying for 40 hours, heating to inactivate enzyme after saccharifying is finished, and filtering to obtain maltose liquid and waste residue B; adding 0.5% of active carbon into the maltose liquid, decoloring for 30min at 80-85 ℃, carrying out ion exchange by using a cation-anion-cation-anion exchange column, and concentrating to 80% by using a four-effect concentrator to obtain the high maltose syrup with the maltose content of 78.5%. The waste residue B and the collected centrifugate were mixed in a ratio of 1: 10, filtering to obtain filtrate and filter residues, precipitating the filtrate with ethanol at room temperature, filtering again, freeze-drying the two parts of filter residues at the temperature of between 15 and 20 ℃ below zero, and sieving the filter residues with a 100-mesh standard sieve to obtain the dietary fiber with the effective component content of 76.88 percent, the water content of 10.5 percent and the water swelling property of 17.34 mL/g.
Comparative example 1
The corn starch processing waste residue (water content 85.0%, starch content 45.0%) is appropriately crushed in a ratio of 1: 1, adding water, uniformly mixing, stirring at 70-80 ℃ for 30min, cooling to 35-40 ℃, and performing primary filtration to obtain filtrate and waste residue A; centrifuging the filtrate to obtain dehydrated corn starch milk and centrifugate; mixing the waste residue A and the corn starch milk, mixing the mixture to a concentration of 20%, adjusting the pH value to 5.5, adding 0.2kg/t of alpha-amylase into the mixture, keeping the temperature at 50-65 ℃ for 40min, and heating to inactivate the enzyme for 5 min; liquefying to obtain a feed liquid with a DE value of 25, adding 3kg/t of dry starch by using saccharifying enzyme, controlling the saccharifying temperature to be 50-65 ℃, saccharifying for 30 hours, heating to inactivate enzyme after saccharifying is finished, and filtering to obtain maltose liquid and waste residue B; adding 0.2% of activated carbon into the maltose syrup liquid, decoloring for 20min at the temperature of 80-85 ℃, performing ion exchange through a cation-anion-cation-anion exchange column, and concentrating to the concentration of 70% by adopting a four-effect concentrator to obtain the maltose syrup with the maltose content of 67.3%. Waste B and collected centrifugate were mixed at a ratio of 1: 10, obtaining filtrate and filter residues, carrying out alcohol precipitation treatment on the filtrate at room temperature, carrying out suction filtration again, finally freeze-drying the two parts of filter residues at the temperature of between 15 ℃ below zero and 20 ℃ below zero, and sieving the filter residues by a standard sieve of 100 meshes to obtain the dietary fiber with the effective component content of 67.2%, the water content of 7.1% and the water absorption expansibility of 15.3 mL/g.
Comparative example 2
The corn starch processing waste residue (the water content is 85.0 percent, the starch content is 45.0 percent) is properly crushed, and the weight ratio of the corn starch processing waste residue to the starch processing waste residue is 1: 1, adding water, mixing uniformly, stirring at 70-80 ℃ for 30min, cooling to 35-40 ℃, adjusting the pH to 5.5, adding 0.5% protease, treating for 3h, heating to inactivate enzyme, and primarily filtering to obtain filtrate and waste residue A; centrifuging the filtrate to obtain dehydrated corn starch milk and centrifugate; mixing the waste residue A and the corn starch milk, mixing the mixture to a concentration of 20%, adjusting the pH value to 5.5, adding 0.2kg/t of alpha-amylase into the mixture, keeping the temperature at 50-65 ℃ for 40min, and heating to inactivate the enzyme for 5 min; liquefying to obtain a feed liquid with a DE value of 25, adding 3kg/t of dry starch by using saccharifying enzyme, controlling the saccharifying temperature to be 50-65 ℃, saccharifying for 30 hours, heating to inactivate the enzyme after saccharifying is finished, and filtering to obtain maltose liquid and waste residue B; adding 0.2% of activated carbon into the maltose syrup liquid, decoloring for 20min at the temperature of 80-85 ℃, performing ion exchange through a cation-anion-cation-anion exchange column, and concentrating to the concentration of 70% by adopting a four-effect concentrator to obtain the maltose syrup with the maltose content of 68.7%. The waste residue B and the collected centrifugate were mixed in a ratio of 1: 10 and water, filtering, freeze-drying the obtained filter residue at-15 to-20 ℃, and screening by a standard sieve of 100 meshes to obtain the dietary fiber with the effective component content of 68.1 percent, the water content of 10.4 percent and the water absorption expansibility of 13.57 mL/g.
As can be seen from the example 1, the comparative example 1 and the comparative example 2, the waste residue is crushed, added with water, mixed uniformly, added with the protease, and then subjected to the processes of suction filtration, alcohol precipitation and the like, thereby not only providing a more compatible substrate for preparing the high maltose syrup, improving the purity of the maltose syrup, but also improving the quality of the prepared dietary fiber.
The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention should not be limited thereby, and modifications and equivalent variations made by those skilled in the art can be made without departing from the scope of the present invention.
Claims (7)
1. A production method for preparing high maltose syrup and dietary fiber by utilizing corn starch processing waste residues is characterized by comprising the following steps:
(1) properly crushing the corn starch processing waste residue, adding water, mixing uniformly, stirring at 70-80 ℃ for 30-50 min, cooling to 35-40 ℃, adjusting the pH to 5.5-7.0, adding protease for treatment for 3h, heating to inactivate enzyme, and primarily filtering to obtain filtrate and waste residue A;
(2) centrifuging the filtrate to obtain dehydrated corn starch milk and centrifugate;
(3) mixing the waste residue A and the corn starch milk in the step (2), adjusting the pH value to 5.5-6.5, adding alpha-amylase, keeping the temperature at 50-65 ℃ for 40min, and heating to inactivate the enzyme for 5 min;
(4) adding saccharifying enzyme into the liquefied feed liquid, controlling the saccharifying temperature to be 50-65 ℃, controlling the saccharifying time to be 30-40 h, heating to inactivate enzyme after saccharifying is finished, and filtering to obtain maltose liquid and waste residue B;
(5) refining the maltose liquid through a series of steps of decoloring, ion exchange and concentration to obtain high maltose syrup;
(6) and uniformly mixing the waste residue B and the collected centrifugate with water, carrying out suction filtration to obtain filtrate and filter residues, carrying out alcohol precipitation treatment on the filtrate at room temperature, carrying out suction filtration again, and finally drying, crushing and sieving the two filter residues to obtain the dietary fiber.
2. The method for producing high maltose syrup and dietary fiber from corn starch processing residues as claimed in claim 1, wherein the corn starch processing residues used in step (1) are selected from 1: 1, adding water, mixing, and adding 0.5-1% of protease.
3. The method for preparing high maltose syrup and dietary fiber from corn starch processing residue as claimed in claim 1, wherein the step (2) is performed by using a horizontal scraper centrifuge.
4. The production method for preparing high maltose syrup and dietary fiber by using the corn starch processing waste residue as claimed in claim 1, wherein the starch concentration after size mixing in step (3) is 20% -30%, 0.2-0.5 kg/t dry starch of alpha-amylase is added, and the liquefied DE value is controlled to be 25-40.
5. The method for preparing high maltose syrup and dietary fiber from corn starch processing waste residue as claimed in claim 1, wherein the adding amount of saccharifying enzyme in step (4) is 3-5 kg/t dry starch.
6. The production method for preparing high maltose syrup and dietary fiber by using the corn starch processing waste residue as claimed in claim 1, wherein 0.2% -0.5% of activated carbon is added in the step (5) for decolorization at 80-85 ℃ for 20-30 min, ion exchange is carried out by a cation-anion-cation-anion exchange column, and concentration is carried out to 70% -80% by using a four-effect concentrator, so as to obtain the high maltose syrup.
7. The method for producing high maltose syrup and dietary fiber from corn starch processing waste residue as claimed in claim 1, wherein the step (6) is carried out in a ratio of 1: 10, mixing with water, freezing and drying at-15 to-20 ℃, and sieving by a standard sieve of 100 meshes to obtain the dietary fiber with the water content of 5-12 percent.
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