CN106701862A - Method for producing arabinose by utilizing maize leaves - Google Patents
Method for producing arabinose by utilizing maize leaves Download PDFInfo
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- CN106701862A CN106701862A CN201611195282.0A CN201611195282A CN106701862A CN 106701862 A CN106701862 A CN 106701862A CN 201611195282 A CN201611195282 A CN 201611195282A CN 106701862 A CN106701862 A CN 106701862A
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K13/00—Sugars not otherwise provided for in this class
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Abstract
The invention relates to a method for producing arabinose, and in particular relates to a method for producing arabinose by utilizing maize leaves. The method comprises the following steps: taking the maize leaves as raw materials, crushing the maize leaves, performing high-temperature steaming, and performing high-temperature clarification, color removal and impurity removal so as to obtain xylan liquid; performing enzymolysis on the xylan liquid by using xylanase so as to obtain arabinose liquid; performing alcoholic fermentation on the arabinose liquid to remove glucose and xylose, and performing microfiltration purification and adsorption decolorization so as to obtain refined arabinose liquid; concentrating the arabinose liquid so as to obtain arabinose syrup; and crystallizing the arabinose syrup or performing spray drying, thereby obtaining the arabinose powder. The method disclosed by the invention has the advantages that 1, the production cost is low, and the product quality is high; 2, the processes are reasonable, temperatures in the processes are sequentially reduced from a high temperature of 126 DEG C to a low temperature, the pH value is increased from 3 of feed liquid during steaming to 6, and the energy and acid can be saved; 3, the xylanase with the pH value of 4.8-6.0 is selected, and the pH value of the feed liquid does not need to be specially regulated during enzymolysis; and 4, the method is suitable for large-scale industrial production.
Description
Technical field
The present invention relates to the production method of arabinose, specifically, it is related to a kind of using maize leaves production arabinose
Method.
Background technology
Arabinose is also referred to as pectinose, is a kind of important functional food, except with low-heat, stabilization, safety, nothing
Outside the good physicochemical characteristicses such as poison, also with the unique physiology work(for promoting the breeding of beneficial bacterium in enteron aisle, suppressing harmful bacteria growing
Can, cause global extensive concern.Arabinose has without carious tooth, not by human consumption and the body that can significantly rise in value
Interior Bifidobacterium is beneficial to intestinal health.Arabinose can reduce toxic metabolic products and be formed, and greatly reduce liver and decompose poison
The burden of element, there is liver-protective healthcare function.Arabinose can promote absorption of the human body to calcium, reduce blood pressure and serum courage
Sterol, strengthens immunity of organisms, anticancer, antifatigue etc. healthcare function.At present, the market sales volume of domestic and international arabinose all exists
Rapid growth.
The cultivation history of China's corn is about more than 470 year.Current China's sown area at 300,000,000 mu or so, be only second to rice,
Wheat, occupies the 3rd in cereal crops, and the U.S. is only second in the world.After corn maturation and results, maize leaves connection corn stalk
Stalk is by substantial amounts of burning, or abandons and make fertilizer, the wasting of resources in Tanaka's natural degradation.If extracting arabinose with it, make resource
It is fully used, environment is protected again, can also produces good economic benefit.
Currently, both at home and abroad to being carried out using raw material arabinoses such as corncob, bagasse, rice husk, stalk, birch, boll hulls
Substantial amounts of research, and, it is that raw material produces arabinose that the country is substantially all with corncob.With maize leaves as raw material production Ah
The technology of uncle's sugar is drawn, is also rarely reported both at home and abroad.
The content of the invention
It is an object of the present invention to provide a kind of method that utilization maize leaves produce arabinose, to solve above-mentioned skill
Art problem.
To solve the above problems, the technical solution adopted in the present invention is：
A kind of method that utilization maize leaves produce arabinose, it is characterised in that：With maize leaves as raw material, through maize leaves powder
Broken, thermophilic digestion and high temperature clarification decolouring removal of impurities obtain xylan liquid；Xylan liquid is digested with zytase obtain arabinose
Liquid；Arabic liquid glucose removes glucose and xylose through alcohol fermentation, then obtains smart Arab's liquid glucose through micro-filtration purification and adsorption bleaching；Ah
Arabinose syrup is can obtain after drawing primary liquid glucose concentration；Arabinose syrup is crystallized or spray drying obtains arabinose powder.
Further say that the method that the utilization maize leaves produce arabinose is comprised the following steps：
1st, maize leaves are crushed to more than 100 mesh；Maize leaves after crushing are soaked in the distilled water that temperature is 60 DEG C
12 hours, distilled water was 10: 1 with the weight ratio of maize leaves；After the completion of immersion, maize leaves are taken out, soaking water is placed in 126 DEG C of temperature
Sealed steam cooking 1 hour under the conditions of degree, obtains boiling feed liquid；
2nd, thermophilic digestion feed liquid is isolated with earthenware Microfilter, buffered tank pumps into High-speed clarification tank, in pipeline mixing
Milk of lime is added in device and adjusts pH value to 10.5, pH value is 5.0 when the acid that phosphorates makes boiling feed liquid be cooled to 50 DEG C, and by boiling material
50~100ppm addition flocculants of liquid measure, clarified tank clarified separation removal leaf slag, mud scum, protein, sugarcane fat, sugarcane are cured and big
The pigment of molecule, obtains supernatant；
3rd, the supernatant of settling tank is taken out, enzymolysis obtains Arabic liquid glucose；Enzymolysis uses zytase, operating condition：pH
Value 4.0~6.5,45~65 DEG C of temperature, 2~10h of time；
4th, alcohol fermentation tank will be sent after Arabic liquid glucose cooling obtained in step 3, by yeast zymotechnique, by arabinose
Glucose and xylose in liquid is fermented into ethanol；32~38 DEG C of fermentation temperature, pH value 4.8~5.2, enzymolysis to grape residual sugar is less than
0.5%；
5th, bleaching process is sent to decolourize after step 4 is purified except the Arabic liquid glucose after miscellaneous sugar through micro-filtration, decolouring uses acid out
Decolouring, decolorizing with activated carbon or/and ion-exchange resin decolorization；
6th, conventional vacuum concentration, crystallization are carried out through the Arabic liquid glucose after decolouring or is spray-dried, you can obtained Arabic
Sugar crystal.
Furtherly, zytase described in step 3 use pH value for 4.8~6.0 zytase.
Beneficial effect：Compared with prior art, the advantage of the invention is that：1st, with maize leaves as raw material, cost of material is low,
Sulfuric acid and caustic soda are not used, and technological process is easy, low production cost, superior product quality；2nd, rational technology, each operation is by 126 DEG C
High temperature order is down to low temperature, pH value then by boiling when feed liquid 3 orders rise to 6, energy-conservation, section acid；3rd, from pH value be 4.8~
6.0 zytase, feed liquid is without specially adjusting pH value during enzymolysis；4th, the equipment used by each step of technological process is all existing
Common equipment in technology, is adapted to industrial-scale production.
Specific embodiment
With reference to specific embodiment, the present invention will be further described.
Embodiment 1：
The method for being produced arabinose described in the present embodiment using maize leaves, is comprised the following steps：
1st, maize leaves are crushed to more than 100 mesh；Maize leaves after crushing are soaked in the distilled water that temperature is 60 DEG C
12 hours, distilled water was 10: 1 with the weight ratio of maize leaves；After the completion of immersion, maize leaves are taken out, soaking water is placed in 126 DEG C of temperature
Sealed steam cooking 1 hour under the conditions of degree, obtains boiling feed liquid；
2nd, thermophilic digestion feed liquid is isolated with earthenware Microfilter, buffered tank pumps into High-speed clarification tank, in pipeline mixing
Milk of lime is added in device and adjusts pH value to 10.5, pH value is 5.0 when the acid that phosphorates makes 50 DEG C of boiling feed liquid, and by boiling reduction of feed volume
50~100ppm adds PAM flocculants, clarified tank clarified separation removal leaf slag, mud scum, protein, sugarcane fat, cured and big point of sugarcane
The pigment of son, obtains supernatant；
3rd, the supernatant for taking out settling tank pumps into enzymatic vessel, when solvent and solute weight ratio is 13: 1 during pH is 6.0, supernatant,
Novi's letter fungal xylanases are added, enzyme dosage is 40U/g butts, is digested under the conditions of temperature 60 C, the reaction time 2.5
Hour, obtain the Arabic liquid glucose that pH value is about 6.0；
4th, the Arabic liquid glucose that step 3 enzymolysis is obtained is cooled to 32~35 DEG C, feeding is equipped with the female alcohol hair of immobilization alcohol
Fermentation tank, pump circulation about 18~22 hours maintains 32~38 DEG C of fermentation jar temperature, and alcoholysis to grape residual sugar is less than 0.5%；
5th, after the Arabic liquid glucose taken out by alcohol fermentation tank is purified through Microfilter, after being decolourized through ion exchange column
Arabic liquid glucose；
6th, the Arabic liquid glucose after decolourizing send vacuum concentration drying, when arabinose solution is concentrated to the 1/4 of original volume
When, add ethanol, the precipitation such as organic acid, furfural is filtered to remove, continue to be concentrated into arabinose content reach 32% (W/V, it is right
Solid content) heavy syrup is obtained, the arabinose crystal of white is can obtain after cooling, wash away surface with a small amount of ethanol solution
Mother liquor, obtains arabinose product.
Embodiment 2：
The method for being produced arabinose described in the present embodiment using maize leaves, is comprised the following steps：
1st, maize leaves are crushed to more than 100 mesh；Maize leaves after crushing are soaked in the distilled water that temperature is 60 DEG C
12 hours, distilled water was 10: 1 with the weight ratio of maize leaves；After the completion of immersion, maize leaves are taken out, soaking water is placed in 126 DEG C of temperature
Sealed steam cooking 1 hour under the conditions of degree, obtains boiling feed liquid；
2nd, thermophilic digestion feed liquid is isolated with earthenware Microfilter, buffered tank pumps into High-speed clarification tank, in pipeline mixing
Milk of lime is added in device and adjusts pH value to 10.5, pH value is 5.0 when the acid that phosphorates makes 50 DEG C of boiling feed liquid, and by boiling reduction of feed volume
50~100ppm adds PAM flocculants, clarified tank clarified separation removal leaf slag, mud scum, protein, sugarcane fat, cured and big point of sugarcane
The pigment of son, obtains supernatant；
3rd, the supernatant for taking out settling tank pumps into enzymatic vessel, and solvent and solute weight ratio is 13 in pH value 4.8~5.2, supernatant:
When 1, Richter scale wood enzyme zytase is added, enzyme dosage is 40U/g butts, is digested under conditions of being 48~52 DEG C in temperature,
In 10 hours reaction time, obtain the Arabic liquid glucose that pH value is about 5.2；
4th, the Arabic liquid glucose that step 3 enzymolysis is obtained is cooled to 32~35 DEG C, feeding is equipped with the female alcohol hair of immobilization alcohol
Fermentation tank, pump circulation about 18~22 hours maintains 32~38 DEG C of fermentation jar temperature, and alcoholysis to grape residual sugar is less than 0.5%；
5th, after the Arabic liquid glucose taken out by alcohol fermentation tank is purified through Microfilter, after being decolourized through ion exchange column
Arabic liquid glucose；
6th, the Arabic liquid glucose after decolourizing send vacuum concentration drying, when arabinose solution is concentrated to the 1/4 of original volume
When, add ethanol, the precipitation such as organic acid, furfural is filtered to remove, continue to be concentrated into arabinose content reach 32% (W/V, it is right
Solid content) heavy syrup is obtained, the arabinose crystal of white is can obtain after cooling, wash away surface with a small amount of ethanol solution
Mother liquor, obtains arabinose product.
Embodiment 3：
1st, maize leaves are crushed to more than 100 mesh；Maize leaves after crushing are soaked in the distilled water that temperature is 60 DEG C
12 hours, distilled water was 10: 1 with the weight ratio of maize leaves；After the completion of immersion, maize leaves are taken out, soaking water is placed in 126 DEG C of temperature
Sealed steam cooking 1 hour under the conditions of degree, obtains boiling feed liquid；
2nd, thermophilic digestion feed liquid is isolated with earthenware Microfilter, buffered tank pumps into High-speed clarification tank, in pipeline mixing
Milk of lime is added in device and adjusts pH value to 10.5, pH value is 5.0 when the acid that phosphorates makes 50 DEG C of boiling feed liquid, and by boiling reduction of feed volume
50~100ppm adds PAM flocculants, clarified tank clarified separation removal leaf slag, mud scum, protein, sugarcane fat, cured and big point of sugarcane
The pigment of son, obtains supernatant；
3rd, the supernatant for taking out settling tank pumps into enzymatic vessel, when solvent and solute weight ratio is 13: 1 during pH is 6.0, supernatant,
Novi's letter fungal xylanases are added, enzyme dosage is 40U/g butts, is digested under the conditions of temperature 60 C, the reaction time 2.5
Hour, obtain the Arabic liquid glucose that pH value is about 6.0；
4th, the Arabic liquid glucose that step 3 enzymolysis is obtained is cooled to 32~35 DEG C, feeding is equipped with the female alcohol hair of immobilization alcohol
Fermentation tank, pump circulation about 18~22 hours maintains 32~38 DEG C of fermentation jar temperature, and alcoholysis to grape residual sugar is less than 0.5%；
5th, after the Arabic liquid glucose taken out by alcohol fermentation tank is purified through Microfilter, obtained with the combined modified activated carbon decolorizing of acid out
Refined Arab's liquid glucose；Arabinose clear liquid pH value to 3.0 is adjusted with hydrochloric acid, arabinose clear liquid temperature is adjusted to 30~80 DEG C, it is excellent
80 DEG C are selected, 20 minutes are stood after gentle agitation；Centrifuge crude separation is used, centrifuge clear liquid is separated through earthenware Microfilter essence again,
Obtain acid out destainer, percent of decolourization about 30%；The operating condition of decolorizing with activated carbon：Amination modified activated carbon dosage 6~12%, it is excellent
Select 10% (w/v, activated carbon/solution), initial pH value 3.0,80 DEG C of bleaching temperature, bleaching time 100 minutes, percent of decolourization is about
55.06%~68.96%.
6th, ion exchange resin deacidification, can be using known resin (such as 732 resins and D301 resins), and technique can be used appoints
What known technique；
7th, the Arabic liquid glucose after decolourizing send vacuum concentration drying, when arabinose solution is concentrated to the 1/4 of original volume
When, add ethanol, the precipitation such as organic acid, furfural is filtered to remove, continue to be concentrated into arabinose content reach 32% (W/V, it is right
Solid content) heavy syrup is obtained, the arabinose crystal of white is can obtain after cooling, wash away surface with a small amount of ethanol solution
Mother liquor, obtains arabinose product.
Embodiment 4：
The method for being produced arabinose described in the present embodiment using maize leaves, is comprised the following steps：
1st, maize leaves are crushed to more than 100 mesh；Maize leaves after crushing are soaked in the distilled water that temperature is 60 DEG C
12 hours, distilled water was 10: 1 with the weight ratio of maize leaves；After the completion of immersion, maize leaves are taken out, soaking water is placed in 126 DEG C of temperature
Sealed steam cooking 1 hour under the conditions of degree, obtains boiling feed liquid；
2nd, thermophilic digestion feed liquid is isolated with earthenware Microfilter, buffered tank pumps into High-speed clarification tank, in pipeline mixing
Milk of lime is added in device and adjusts pH value to 10.5, pH value is 5.0 when the acid that phosphorates makes 50 DEG C of boiling feed liquid, and by boiling reduction of feed volume
50~100ppm adds PAM flocculants, clarified tank clarified separation removal leaf slag, mud scum, protein, sugarcane fat, cured and big point of sugarcane
The pigment of son, obtains supernatant；
3rd, the supernatant for taking out settling tank pumps into enzymatic vessel, and solvent and solute weight ratio is 13 in pH value 4.8~5.2, supernatant:
When 1, Richter scale wood enzyme zytase is added, enzyme dosage is 40U/g butts, is digested under conditions of being 48~52 DEG C in temperature,
In 10 hours reaction time, obtain the Arabic liquid glucose that pH value is about 5.2；
4th, the Arabic liquid glucose that step 3 enzymolysis is obtained is cooled to 32~35 DEG C, feeding is equipped with the female alcohol hair of immobilization alcohol
Fermentation tank, pump circulation about 18~22 hours maintains 32~38 DEG C of fermentation jar temperature, and alcoholysis to grape residual sugar is less than 0.5%；
5th, after the Arabic liquid glucose taken out by alcohol fermentation tank is purified through Microfilter, obtained with the combined modified activated carbon decolorizing of acid out
Refined Arab's liquid glucose；Arabinose clear liquid pH value to 3.0 is adjusted with hydrochloric acid, arabinose clear liquid temperature is adjusted to 30~80 DEG C, it is excellent
80 DEG C are selected, 20 minutes are stood after gentle agitation；Centrifuge crude separation is used, centrifuge clear liquid is separated through earthenware Microfilter essence again,
Obtain acid out destainer, percent of decolourization about 30%；The operating condition of decolorizing with activated carbon：Amination modified activated carbon dosage 6~12%, it is excellent
10% (w/v, activated carbon/solution) is selected, initial pH value is 3.0,80 DEG C of bleaching temperature, bleaching time 100min, and percent of decolourization is about
55.06%~68.96%.
6th, ion exchange resin deacidification, can be using known resin, such as 732 resins and D301 resins, and technique can be used appoints
What known technique.
7th, the Arabic liquid glucose after decolourizing send vacuum concentration drying, when arabinose solution is concentrated to the 1/4 of original volume
When, add ethanol, the precipitation such as organic acid, furfural is filtered to remove, continue to be concentrated into arabinose content reach 32% (W/V, it is right
Solid content) heavy syrup is obtained, the arabinose crystal of white is can obtain after cooling, wash away surface with a small amount of ethanol solution
Mother liquor, obtains arabinose product.
The above is only the preferred embodiment of the present invention, for those skilled in the art,
Without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should be regarded as this hair
Bright protection domain.
Claims (3)
1. a kind of method that utilization maize leaves produce arabinose, it is characterised in that：With maize leaves as raw material, through maize leaves powder
Broken, thermophilic digestion and high temperature clarification decolouring removal of impurities obtain xylan liquid；Xylan liquid is digested with zytase obtain arabinose
Liquid；Arabic liquid glucose removes glucose and xylose through alcohol fermentation, then obtains smart Arab's liquid glucose through micro-filtration purification and adsorption bleaching；Ah
Arabinose syrup is can obtain after drawing primary liquid glucose concentration；Arabinose syrup is crystallized or spray drying obtains arabinose powder.
2. the method for producing arabinose using maize leaves according to claim 1, it is characterised in that：Comprise the following steps：
(1) maize leaves are crushed to more than 100 mesh；Maize leaves after crushing are soaked in the distilled water that temperature is 60 DEG C 12 small
When, distilled water is 10: 1 with the weight ratio of maize leaves；After the completion of immersion, maize leaves are taken out, soaking water is placed in 126 DEG C of temperature strips
Sealed steam cooking 1 hour under part, obtains boiling feed liquid；
(2) thermophilic digestion feed liquid is isolated with earthenware Microfilter, buffered tank pumps into High-speed clarification tank, in pipe-line mixer
Addition milk of lime adjusts pH value to 10.5, and pH value is 5.0 when the acid that phosphorates makes boiling feed liquid be cooled to 50 DEG C, and by boiling reduction of feed volume
50~100ppm addition flocculants, clarified tank clarified separation removal leaf slag, mud scum, protein, sugarcane fat, sugarcane be cured and macromolecular
Pigment, obtain supernatant；
(3) supernatant for taking out settling tank pumps into enzymatic vessel, and enzymolysis obtains Arabic liquid glucose；Enzymolysis uses zytase, operates
Condition：PH value 4.0~6.5,45~65 DEG C of temperature, 2~10h of time；
(4) alcohol fermentation tank will be sent after Arabic liquid glucose cooling obtained in step (3), by yeast zymotechnique, by arabinose
Glucose and xylose in liquid is fermented into ethanol；32~38 DEG C of fermentation temperature, pH value 4.8~5.2, enzymolysis to grape residual sugar is less than
0.5%；
(5) by step (4) except miscellaneous sugar after Arabic liquid glucose through micro-filtration purify after send bleaching process to decolourize, decolourize using acid out take off
Color, decolorizing with activated carbon or/and ion-exchange resin decolorization；
(6) conventional vacuum concentration, crystallization are carried out through the Arabic liquid glucose after decolouring or is spray-dried, you can obtain arabinose brilliant
Body.
3. the method for producing arabinose using maize leaves according to claim 2, it is characterised in that：Described in step (3)
Zytase use pH value for 4.8~6.0 zytase.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116655819A (en) * | 2023-06-07 | 2023-08-29 | 湖南广霖生物科技有限公司 | Production process and production device of instant acacia powder |
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| CN101100685B (en) * | 2007-08-01 | 2010-06-09 | 李萍 | Method for preparing L-arabinose |
| CN101974431A (en) * | 2010-10-08 | 2011-02-16 | 天津科技大学 | Method for producing single cell proteins and L-arabinose by corn bran |
| CN101979640A (en) * | 2010-10-18 | 2011-02-23 | 广西大学 | Method for producing xylooligosaccharide by using sugarcane leaves |
| CN102286571A (en) * | 2011-06-16 | 2011-12-21 | 北京化工大学 | Clean and high-efficiency production process for preparing xylose and L-arabinose |
-
2016
- 2016-12-22 CN CN201611195282.0A patent/CN106701862A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101100685B (en) * | 2007-08-01 | 2010-06-09 | 李萍 | Method for preparing L-arabinose |
| CN101974431A (en) * | 2010-10-08 | 2011-02-16 | 天津科技大学 | Method for producing single cell proteins and L-arabinose by corn bran |
| CN101979640A (en) * | 2010-10-18 | 2011-02-23 | 广西大学 | Method for producing xylooligosaccharide by using sugarcane leaves |
| CN102286571A (en) * | 2011-06-16 | 2011-12-21 | 北京化工大学 | Clean and high-efficiency production process for preparing xylose and L-arabinose |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116655819A (en) * | 2023-06-07 | 2023-08-29 | 湖南广霖生物科技有限公司 | Production process and production device of instant acacia powder |
| CN116655819B (en) * | 2023-06-07 | 2024-06-11 | 湖南广霖生物科技有限公司 | Production process and production device of instant acacia powder |
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Application publication date: 20170524 |