CN107034318A - A kind of processing method of xylose hydrolysis fluid - Google Patents
A kind of processing method of xylose hydrolysis fluid Download PDFInfo
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- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
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Abstract
The invention discloses a kind of processing method of xylose hydrolysis fluid, belong to technical field of xylose production.The processing method of the present invention is concretely comprised the following steps：(1) xylose hydrolysis fluid is subjected to primary purification, obtains primary purification liquid (2) and primary purification liquid is subjected to removal of impurities, removal of impurities liquid is carried out secondary refining, (4) crystallization by (3).The processing method of xylose hydrolysis fluid of the present invention, the galactolipin in xylose hydrolysis fluid is eliminated by enzymatic isolation method, improves the purity of xylose in hydrolyzate, and improves crystallization yield of xylose；The hydrogen peroxide produced in enzymolysis process of the present invention can be as bactericide, and solving the refined rear easily microbiological contamination of hydrolyzate causes the problem of thing is grown in appearance；The technique that galactolipin is removed by using enzyme, solves the more difficult control of process conditions, galactolipin when yeast fermentation removes galactolipin and removes halfway problem；The problem of technique for removing galactolipin using liquid enzymes solves sad filter when yeast fermentation removes galactolipin.
Description
Technical field
The present invention relates to technical field of xylose production, more particularly to a kind of processing method of xylose hydrolysis fluid.
Background technology
At present, xylose is more extracts from agriculture and forestry organic waste material (such as corncob, bagasse, bamboo, birch-bark, straw), tool
Body technology is, by acid hydrolyzation or fermentation method, by the hydrolysis of hemicellulose in agriculture and forestry organic waste material into after monose, carries out refined carries
It is pure.And galactosyl is usually contained in the hemicellulose chain in these agriculture and forestry organic waste materials, therefore can not in hydrolyzate after hydrolysis
What is avoided generates miscellaneous sugar galactolipin.It is well known that solute purity is higher during crystallization, crystallization yield is higher, and galactolipin is deposited
The purity of xylose can be being reduced, crystallization yield of xylose is reduced.Contain the half of about 3%-7% in usual agriculture and forestry organic waste material hydrolyzate
Lactose, xylose purity is about 75%-80%, and crystallization yield of xylose is relatively low, and about 50%.
In addition, during xylose production, the problem of there is microbiological contamination in middle feed liquid, substantial amounts of growth thing be enriched in it is refined after
Xylose in, and growth and breeding is very fast so that xylose refines fluid viscosity and becomes big, have impact on crystallization yield of xylose, adds clear
Except the workload for growing thing.If adding bactericide to suppress the growth of growth thing, there is the limitation of food security aspect, and increase
Production cost is added.
Patent《A kind of method that utilization biological treatment improves crystalline xylose yield》(number of patent application
201310333481.3) a kind of method that utilization yeast fermentation method removes galactolipin, but actually not industrialization have been recorded in,
Reason is the more difficult control of yeast fermentation condition simultaneously of yeast fermentative degradation galactolipin, once condition control is bad, is easily caused yeast
Death, and there is the hidden danger of microbiological contamination, while also there is filtration difficulty, galactolipin removes halfway problem.
The content of the invention
The shortcoming of prior art in view of the above, it is an object of the invention to provide a kind of processing side of xylose hydrolysis fluid
Method, overcoming present in prior art impurity galactolipin influence xylose purity to make, crystallization yield of xylose is low, xylose hydrolysis fluid is refined
Afterwards easy microbiological contamination cause to produce grow the more difficult control of thing, process conditions, galactolipin remove not thoroughly, zymotic fluid sad filter the problems such as.
To achieve these goals or other purposes, the present invention is achieved by the following technical solutions：
A kind of processing method of xylose hydrolysis fluid, is concretely comprised the following steps：
(1) primary purification：Xylose hydrolysis fluid is taken, by pre- decolouring, anion exchange resin-cationic ion-exchange resin-the moon
Ion exchange resin carries out ion exchange, and then concentration is once decolourized with activated carbon, obtain primary purification liquid；
(2) removal of impurities：The primary purification liquid that step (1) is obtained is put into enzymatic vessel, is passed through compressed air, adds liquid enzymes
Digested, obtain removal of impurities liquid；
(3) secondary refining：Flocculating agent A is added into the removal of impurities liquid of step (2), flocculating agent B is continuously added after stirring,
Flocculation sediment is carried out, activated carbon is then added and carries out secondary decolourization, be filtrated to get secondary decolourization liquid, by secondary decolourization liquid by sun
Ion exchange resin-anion exchange resin carries out ion exchange, obtains secondary refining liquid；
(4) crystallize：The secondary refining liquid concentration that step (3) is obtained, cooling crystallization obtains crystal after centrifugation drying
Xylose.
Further, heretofore described xylose hydrolysis fluid is by agriculture and forestry organic waste material water by the method for acidolysis or fermentation
Solution, the obtained feed liquid rich in xylose.
The agriculture and forestry organic waste material is corncob, bagasse, mao bamboon slag or birch-bark.
The method of the acidolysis or fermentation refers to reaction is hydrolyzed using sulfuric acid, hydrochloric acid or yeast as catalyst.
Further, decolourized in advance using discarded activated carbon in step (1), can fully recycle discarded activated carbon
Remaining decoloring ability, improves activated carbon utilization rate, while being decolourized in advance to feed liquid, the part filtered out in xylose hydrolysis fluid is miscellaneous
Matter, protection is follow-up not to be contaminated from friendship step.
Further, be to remove the sulfate radical in xylose hydrolysis fluid, after xylose hydrolysis fluid is decolourized in advance in step (1) by
Anion exchange resin-cationic ion-exchange resin-anion exchange resin carries out ion exchange, wherein, anion exchange resin
For macroreticular weakly base resin, preferably model D301 resins.Cationic ion-exchange resin is gel-type strong resin, preferably model 001
× 7 resins.
Further, index of refraction 20%-30% is concentrated into step (1), is once decolourized to light transmittance 40%-70%.
Further, the discarded activated carbon produced after once being decolourized in step (1) is recyclable, for pre- decolorization process
In, recycled.
Further, the amount that compressed air is passed through per hour in step (2) is 1-7 times of primary purification liquid product.
Further, liquid enzymes are galactose oxidase or galactose oxidase and peroxidase described in step (2)
Mixture.The galactose oxidase produces hydrogen peroxide in enzymolysis process, effectively inhibits the growth for growing thing, plays
The effect of bactericide, easily microbiological contamination after preventing xylose hydrolysis fluid refined, produces and grows thing.
Preferably, the liquid enzymes refer to the weight of galactose oxidase and peroxidase, wherein galactose oxidase
Part is 0.5-2 parts, and peroxidase parts by weight are 0.5-1 parts.
Further, liquid enzymes addition is the 0.5 ‰ -2 ‰ of primary purification liquid quality in step (2).Preferably, step
(2) liquid enzymes addition is the 1 ‰ of primary purification liquid quality in.
Further, hydrolysis temperature is 20-50 DEG C in step (2), and enzymolysis time is 5-24h, and enzymolysis is gone after terminating
Except the removal of impurities liquid of galactolipin.
Feed liquid is into neutral meta-alkali after resin anion (R.A.) is exchanged for the primary purification liquid obtained due to step (1), therefore, step
Suddenly the enzymolysis process of (2) is not required to adjust pH.
Further, flocculating agent A is the polyaluminium chloride solution that mass percentage concentration is 5% in step (3), and flocculating agent B is
Mass percentage concentration is 0.1% polyacrylamide solution.
Further, the addition of flocculating agent A is the 3%-4% of removal of impurities liquid quality, and the addition of flocculating agent B is removal of impurities liquid
The 3%-4% of quality.The flocculation sediment time is 30min, can be easy to be filtered to remove by enzymolysis product flocculation sediment.
Further, in step (3) secondary decolourization to light transmittance 50%-75%.
Further, the discarded activated carbon produced after step (3) secondary decolourization is recyclable, for the pre- de- of step (1)
In color, recycled.
Further, the cationic ion-exchange resin described in step (3) is gel-type strong resin, preferably model 001 × 7
Resin；Described anion exchange resin is macroreticular weakly base resin, preferably model D301 resins, and the purpose is to further remove
The enzymolysis product of residual.
Further, secondary refining liquid is concentrated into refractive power 85% in step (4).
Further, crystallized in step (4) by the way of cooling crystallization, be cooled to 30 DEG C.
The processing method of xylose hydrolysis fluid of the present invention, the gala in xylose hydrolysis fluid is eliminated by enzymatic isolation method
Sugar, so that the purity of xylose improves 3-7% in hydrolyzate, makes crystallization yield of xylose improve 3-7%, solves traditional handicraft and obtains
Hydrolyzate in xylose purity it is low, the problem of crystallization yield is low；The hydrogen peroxide produced in enzymolysis process of the present invention can be as killing
Microbial inoculum, solving the refined rear easily microbiological contamination of hydrolyzate causes the problem of thing is grown in appearance；The technique that galactolipin is removed by using enzyme, solution
The more difficult control of process conditions, galactolipin remove halfway problem when certainly yeast fermentation removes galactolipin；Removed using liquid enzymes
The problem of technique of galactolipin solves sad filter when yeast fermentation removes galactolipin.
Embodiment
Illustrate embodiments of the present invention below by way of particular specific embodiment, those skilled in the art can be by this explanation
Content disclosed by book understands other advantages and effect of the present invention easily.The present invention can also be by different specific in addition
Embodiment is embodied or practiced, and the various details in this specification can also not carried on the back based on different viewpoints and application
Various modifications or alterations are carried out under spirit from the present invention.It should be noted that, in the case where not conflicting, following examples and reality
Applying the feature in example can be mutually combined.
The preparation of flocculant：Polyaluminium chloride is configured to the polyaluminium chloride solution of mass percent concentration 5%, by cation
Type polyacrylamide is configured to the polyacrylamide solution of mass percent concentration 0.1%, standby.
Prepare xylose hydrolysis fluid standby：Agriculture and forestry organic waste material is hydrolyzed by the method for acidolysis or fermentation, what is obtained is rich in wood
The feed liquid of sugar；Described acidolysis or the method for fermentation refer to reaction is hydrolyzed using sulfuric acid, hydrochloric acid or yeast as catalyst；
Described agriculture and forestry organic waste material is corncob, bagasse, mao bamboon slag or birch-bark.
Embodiment 1
Using corncob as raw material, sulfuric acid is that catalyst progress acidolysis reaction obtains xylose hydrolysis fluid, and its processing method is specific
Step is：
1st, primary purification：Xylose hydrolysis fluid 5kg is taken, is decolourized in advance by discarded activated carbon, after negative and positive anion exchange, concentration
To refractive power 20%, once decolourized with activated carbon afterwards, decolourize to printing opacity 70%, obtain primary purification liquid, production of once decolourizing
Raw discarded activated carbon is back in pre- decolorization process；
Described negative and positive anion exchange be using the cationic ion-exchange resin of D301 anion exchange resin-001 × 7-
The order of D301 anion exchange resin carries out ion exchange；
2nd, removal of impurities：The primary purification liquid that step (1) is obtained, which is put into after enzymatic vessel, is passed through compressed air, per hour intake
It is 7 times of primary purification liquid product, adds galactose oxidase, galactose oxidase addition is primary purification liquid quality
0.5 ‰, enzymolysis is stirred, hydrolysis temperature is 20 DEG C, and enzymolysis time is 24h, makes the miscellaneous sugar galactolipin quilt in primary purification liquid
Remove, obtain removal of impurities liquid；
3rd, secondary refining：The polyaluminium chloride solution that mass percentage concentration is 5% is added into the removal of impurities liquid of step (2), is stirred
The polyacrylamide solution that mass percentage concentration is 0.1% is continuously added after mixing uniformly, flocculation sediment 30min is carried out, will digest
Product flocculation sediment, continuously adds activated carbon by removal of impurities liquid secondary decolourization to printing opacity 75%, and secondary decolourization is obtained after plate-frame filtering
Liquid, the enzymolysis product and salinity that secondary decolourization liquid is crossed in cation and anion exchange resin removing feed liquid, obtains secondary refining liquid, two
The secondary discarded activated carbon for decolourizing to produce is back in the pre- decolorization process of step (1)；
Wherein, the addition for the polyaluminium chloride solution that mass percentage concentration is 5% is the 3% of removal of impurities liquid quality, quality hundred
The addition for the polyacrylamide solution that point concentration is 0.1% is the 4% of removal of impurities liquid quality；
Described cation and anion exchange is the order using 001 × 7 cationic ion-exchange resin-D301 anion exchange resin
Carry out ion exchange；
4th, crystallize：Secondary refining liquid is concentrated into refractive power 85%, the decrease temperature crystalline by the way of crystallisation by cooling is cooled to 30
DEG C, obtain crystal xylose after centrifugation drying.
Embodiment 2
Using bagasse as raw material, yeast is that catalyst progress fermentation process obtains xylose hydrolysis fluid, and its processing method is specific
Step is：
1st, primary purification：Xylose hydrolysis fluid 10t is taken, it is dense after the pre- decolouring of discarded activated carbon and negative and positive anion exchange
Refractive power 30% is reduced to, is once decolourized with activated carbon afterwards, is decolourized to printing opacity 40%, is obtained primary purification liquid, once decolourize
The discarded activated carbon produced is back in pre- decolorization process；
Described negative and positive anion exchange be using the cationic ion-exchange resin of D301 anion exchange resin-001 × 7-
The order of D301 anion exchange resin carries out ion exchange；
2nd, removal of impurities：The primary purification liquid that step (1) is obtained, which is put into after enzymatic vessel, is passed through compressed air, per hour intake
It is 1 times of primary purification liquid product；Galactose oxidase is added, the addition of galactose oxidase is primary purification liquid quality
2 ‰, enzymolysis is stirred, hydrolysis temperature is 20 DEG C, and enzymolysis time is 5h, is removed the miscellaneous sugar galactolipin in primary purification liquid
Remove, obtain removal of impurities liquid；
3rd, secondary refining：The polyaluminium chloride solution that mass percentage concentration is 5% is added into the removal of impurities liquid of step (2), is stirred
The polyacrylamide solution that mass percentage concentration is 0.1% is continuously added after mixing uniformly, flocculation sediment 30min is carried out, will digest
Product flocculation sediment, continuously adds activated carbon by removal of impurities liquid secondary decolourization to printing opacity 50%, and secondary decolourization is obtained after plate-frame filtering
Liquid, the remaining salinity that secondary decolourization liquid is crossed in cation and anion exchange resin removing feed liquid, obtains secondary refining liquid, secondary decolourization
The discarded activated carbon produced is back in the pre- decolorization process of step (1)；
Wherein, the addition for the polyaluminium chloride solution that mass percentage concentration is 5% is the 4% of removal of impurities liquid quality, quality hundred
The addition for the polyacrylamide solution that point concentration is 0.1% is the 3% of removal of impurities liquid quality；
Described cation and anion exchange is the order using 001 × 7 cationic ion-exchange resin-D301 anion exchange resin
Carry out ion exchange；
4th, crystallize：Secondary refining liquid is concentrated into refractive power 78%, the decrease temperature crystalline by the way of crystallisation by cooling is cooled to 30
DEG C, obtain crystal xylose after centrifugation drying.
Embodiment 3
Using mao bamboon slag as raw material, hydrochloric acid is that catalyst progress acidolysis processing obtains xylose hydrolysis fluid, and its processing method is specific
Step is：
1st, primary purification：Xylose hydrolysis fluid 100t is taken, it is dense after the pre- decolouring of discarded activated carbon and negative and positive anion exchange
Refractive power 25% is reduced to, is once decolourized with activated carbon afterwards, is decolourized to printing opacity 60%, is obtained primary purification liquid, once decolourize
The discarded activated carbon produced is back in pre- decolorization process；
Described negative and positive anion exchange be using the cationic ion-exchange resin of D301 anion exchange resin-001 × 7-
The order of D301 anion exchange resin carries out ion exchange；
2nd, removal of impurities：The primary purification liquid that step (1) is obtained, which is put into after enzymatic vessel, is passed through compressed air, per hour intake
It is 5 times of primary purification liquid product, adds galactose oxidase and peroxidase, addition is primary purification liquid quality
1 ‰, digested, hydrolysis temperature is 20 DEG C, and enzymolysis time is 16h, be removed the miscellaneous sugar galactolipin in primary purification liquid, obtain
To removal of impurities liquid；
3rd, secondary refining：The polyaluminium chloride solution that mass percentage concentration is 5% is added into the removal of impurities liquid of step (2), is stirred
The polyacrylamide solution that mass percentage concentration is 0.1% is continuously added after mixing uniformly, flocculation sediment 30min is carried out, will digest
Product flocculation sediment, continuously adds activated carbon by removal of impurities liquid secondary decolourization to printing opacity 60%, and secondary decolourization is obtained after plate-frame filtering
Liquid, the remaining salinity that secondary decolourization liquid is crossed in cation and anion exchange resin removing feed liquid, obtains secondary refining liquid；
Wherein, the addition for the polyaluminium chloride solution that mass percentage concentration is 5% is the 3% of removal of impurities liquid quality, quality hundred
The addition for the polyacrylamide solution that point concentration is 0.1% is the 4% of removal of impurities liquid quality, the discarded activity that secondary decolourization is produced
Charcoal is back in the pre- decolorization process of step (1)；
Described cation and anion exchange is the order using 001 × 7 cationic ion-exchange resin-D301 anion exchange resin
Carry out ion exchange；
4th, crystallize：Secondary refining liquid is concentrated into refractive power 80%, the decrease temperature crystalline by the way of crystallisation by cooling is cooled to 30
DEG C, obtain crystal xylose after centrifugation drying.
Embodiment 4
Using birch-bark as raw material, sulfuric acid is that catalyst progress acidolysis processing obtains xylose hydrolysis fluid, and its processing method is specific
Step is：
1) primary purification：Take 100t xylose hydrolysis fluids, decolourized in advance by discarded activated carbon, negative and positive anion exchange, concentration
To refractive power 25%, once decolourized with activated carbon afterwards, decolourize to printing opacity 60%, obtain primary purification liquid, production of once decolourizing
Raw discarded activated carbon is back in pre- decolorization process；
Described negative and positive anion exchange be using the cationic ion-exchange resin of D301 anion exchange resin-001 × 7-
The order of D301 anion exchange resin carries out ion exchange；
2) removal of impurities：The primary purification liquid that step (1) is obtained, which is put into after enzymatic vessel, is passed through compressed air, per hour intake
It is 5 times of primary purification liquid product, adds galactose oxidase and peroxidase, addition is primary purification liquid quality
1 ‰, digested, hydrolysis temperature is 35 DEG C, and enzymolysis time is 16h, be removed the miscellaneous sugar galactolipin in primary purification liquid, obtain
To removal of impurities liquid；
3) secondary refining：The polyaluminium chloride solution that mass percentage concentration is 5% is added into the removal of impurities liquid of step (2), is stirred
The polyacrylamide solution that mass percentage concentration is 0.1% is continuously added after mixing uniformly, flocculation sediment 30min is carried out, will digest
Product flocculation sediment, continuously adds activated carbon by removal of impurities liquid secondary decolourization to printing opacity 60%, and secondary decolourization is obtained after plate-frame filtering
Liquid, the remaining salinity that secondary decolourization liquid is crossed in cation and anion exchange resin removing feed liquid, obtains secondary refining liquid；
Wherein, the addition for the polyaluminium chloride solution that mass percentage concentration is 5% is the 4% of removal of impurities liquid quality, quality hundred
The addition for the polyacrylamide solution that point concentration is 0.1% is the 4% of removal of impurities liquid quality, the discarded activity that secondary decolourization is produced
Charcoal is back in the pre- decolorization process of step (1)；
Described cation and anion exchange is the order using 001 × 7 cationic ion-exchange resin-D301 anion exchange resin
Carry out ion exchange；
4) crystallize：Secondary refining liquid is concentrated into refractive power 85%, the decrease temperature crystalline by the way of crystallisation by cooling is cooled to 30
DEG C, obtain crystal xylose after centrifugation drying.
Comparative example 1
Using corncob as raw material, sulfuric acid is that catalyst progress acidolysis reaction obtains xylose hydrolysis fluid, and its processing method is not adopted
Digested with liquid enzymes, but sequentially pass through once the step of exquisiteness, secondary refining, crystallization.It is each in hydrolyzate after its primary purification
The purity of component and final crystallization yield, are contrasted with embodiment 1, the results are shown in Table 1 and table 2.
Table 1：Each component comparison or purity table after embodiment 1 and the primary purification of comparative example 1
| Comparative example 1 | Embodiment 1 | |
| Glucose | 5.3% | 5.5% |
| Xylose | 80.7% | 83.8% |
| Galactolipin | 3.7% | 0.0% |
| Arabinose | 9.1% | 9.6% |
| Impurity | 1.1% | 1.1% |
Table 2：The crystallization yield contrast table of embodiment 1 and comparative example 1
| Comparative example 1 | Embodiment 1 | |
| Secondary refining concentrate quality (g) | 1000 | 1000 |
| Crystallize obtained xylose (g) | 530 | 556.36 |
| Xylose moisture | 0.30% | 0.30% |
| Crystallization yield | 52.84% | 55.47% |
Comparative example 2
Using bagasse as raw material, yeast is that catalyst progress fermentation process obtains xylose hydrolysis fluid, and its processing method is not adopted
Digested with liquid enzymes, but sequentially pass through once the step of exquisiteness, secondary refining, crystallization.It is each in hydrolyzate after its primary purification
The purity of component and final crystallization yield, are contrasted with embodiment 2, the results are shown in Table 3 and table 4.
Table 3：Each component comparison or purity table before and after enzymolysis
| Comparative example 2 | Embodiment 2 | |
| Glucose | 7.5% | 9.01% |
| Xylose | 78.6% | 80.17% |
| Galactolipin | 5.7% | 0.00% |
| Arabinose | 5.9% | 8.38% |
| Impurity | 2.3% | 2.44% |
Table 4：Enzymolysis and the crystallization yield contrast table not digested
| Comparative example 2 | Embodiment 2 | |
| Secondary refining concentrate quality (g) | 1000 | 1000 |
| Crystallize obtained xylose (g) | 486.65 | 525.49 |
| Xylose moisture | 0.30% | 0.30% |
| Crystallization yield | 48.52% | 52.39% |
Comparative example 3
Using mao bamboon slag as raw material, hydrochloric acid is that catalyst progress acidolysis processing obtains xylose hydrolysis fluid, and its processing method is not adopted
Digested with liquid enzymes, but sequentially pass through once the step of exquisiteness, secondary refining, crystallization.It is each in hydrolyzate after its primary purification
The purity of component and final crystallization yield, are contrasted with embodiment 3, the results are shown in Table 5 and table 6.
Table 5：Each component comparison or purity table before and after enzymolysis
| Comparative example 3 | Embodiment 3 | |
| Glucose | 4.8% | 5.2% |
| Xylose | 79% | 82.0% |
| Galactolipin | 6.8% | 0.0% |
| Arabinose | 7.6% | 11.4% |
| Impurity | 1.8% | 1.5% |
Table 6：Enzymolysis and the crystallization yield contrast table not digested
| Comparative example 3 | Embodiment 3 | |
| Secondary refining concentrate quality (g) | 1000 | 1000 |
| Crystallize obtained xylose (g) | 493.45 | 540.83 |
| Xylose moisture | 0.30% | 0.30% |
| Crystallization yield | 49.20% | 53.92% |
Comparative example 4
Using birch-bark as raw material, sulfuric acid is that catalyst progress acidolysis processing obtains xylose hydrolysis fluid, and its processing method is not adopted
Digested with liquid enzymes, but sequentially pass through once the step of exquisiteness, secondary refining, crystallization.It is each in hydrolyzate after its primary purification
The purity of component and final crystallization yield, are contrasted with embodiment 4, the results are shown in Table 7 and table 8.
Table 7：Each component comparison or purity table before and after enzymolysis
| Comparative example 4 | Embodiment 4 | |
| Glucose | 6.10% | 6.5% |
| Xylose | 78.30% | 83.0% |
| Galactolipin | 5.70% | 0.0% |
| Arabinose | 8.50% | 9.0% |
| Impurity | 1.40% | 1.5% |
Table 8：Enzymolysis and the crystallization yield contrast table not digested
| Comparative example 4 | Embodiment 4 | |
| Secondary refining concentrate quality (g) | 1000 | 1000 |
| Crystallize obtained xylose (g) | 509.60 | 549.83 |
| Xylose moisture | 0.30% | 0.30% |
| Crystallization yield | 50.81% | 54.82% |
The secondary refining liquid that secondary refining liquid made from embodiment 1-4 and comparative example 1-4 are obtained, is individually positioned in shaking table
In, setting speed 150rpm, 30 DEG C of temperature places whether produce growth thing in observation hydrolyzate after 24h, as a result as shown in table 9.
Table 9：Grow thing and produce the table of comparisons
Summary test result, it can be seen that the present invention after xylitol treatment fluid primary purification by will add liquid
Enzyme is digested, and can remove the galactolipin in hydrolyzate, is improved the purity of xylose in hydrolyzate, is carried crystallization yield of xylose
It is high；Meanwhile, the hydrogen peroxide produced in enzymolysis process can be as bactericide, and solving the refined rear easily microbiological contamination of hydrolyzate causes appearance
The problem of growing thing.
The above-described embodiments merely illustrate the principles and effects of the present invention, not for the limitation present invention.It is any ripe
Know the personage of this technology all can carry out modifications and changes under the spirit and scope without prejudice to the present invention to above-described embodiment.Cause
This, those of ordinary skill in the art is complete without departing from disclosed spirit and institute under technological thought such as
Into all equivalent modifications or change, should by the present invention claim be covered.
Claims (10)
1. a kind of processing method of xylose hydrolysis fluid, it is characterised in that comprise the following steps that：
(1) primary purification：Xylose hydrolysis fluid is taken, is handed over after pre- decolouring through anion exchange resin-cationic ion-exchange resin-anion
Change resin and carry out ion exchange, then concentration is once decolourized with activated carbon, obtain primary purification liquid；
(2) removal of impurities：The primary purification liquid that step (1) is obtained is put into enzymatic vessel, is passed through compressed air, is added liquid enzymes and is carried out
Enzymolysis, obtains removal of impurities liquid；
(3) secondary refining：Flocculating agent A is added into the removal of impurities liquid of step (2), after stirring, flocculating agent B is continuously added, enters
Row flocculation sediment, then add activated carbon carry out secondary decolourization, be filtrated to get secondary decolourization liquid, by secondary decolourization liquid by sun from
Sub-exchange resin-anion exchange resin carries out ion exchange, obtains secondary refining liquid；
(4) crystallize：The secondary refining liquid concentration that step (3) is obtained, cooling crystallization obtains crystal wood after centrifugation drying
Sugar.
2. processing method according to claim 1, it is characterised in that step is concentrated into index of refraction 20%-30% in (1),
Once decolourize to light transmittance 40%-70%.
3. processing method according to claim 1, it is characterised in that the amount that compressed air is passed through per hour in step (2)
It is 1-7 times of primary purification liquid product.
4. processing method according to claim 1, it is characterised in that liquid enzymes are galactose oxidases described in step (2)
The mixture of enzyme or galactose oxidase and peroxidase.
5. processing method according to claim 4, it is characterised in that the liquid enzymes are galactose oxidase and peroxidating
The parts by weight of the mixture of thing enzyme, wherein galactose oxidase are 0.5-2 parts, and peroxidase parts by weight are 0.5-1 parts.
6. processing method according to claim 1, it is characterised in that liquid enzymes addition is primary purification in step (2)
The 0.5 ‰ -2 ‰ of liquid quality.
7. processing method according to claim 1, it is characterised in that hydrolysis temperature is 20-50 DEG C in step (2), enzymolysis
Time is 5-24h.
8. processing method according to claim 1, it is characterised in that flocculating agent A is that mass percentage concentration is in step (3)
5% polyaluminium chloride solution, flocculating agent B is the polyacrylamide solution that mass percentage concentration is 0.1%.
9. processing method according to claim 1, it is characterised in that the addition of flocculating agent A is removal of impurities liquid in step (3)
The 3%-4% of quality, the addition of flocculating agent B is the 3%-4% of removal of impurities liquid quality.
10. processing method according to claim 1, it is characterised in that step 3) described in cationic ion-exchange resin be solidifying
Glue-type strong resin, the anion exchange resin is macroreticular weakly base resin.
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| CN201611078864.0A CN107034318A (en) | 2016-11-30 | 2016-11-30 | A kind of processing method of xylose hydrolysis fluid |
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| CN201611078864.0A Pending CN107034318A (en) | 2016-11-30 | 2016-11-30 | A kind of processing method of xylose hydrolysis fluid |
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| CN108004351A (en) * | 2017-12-08 | 2018-05-08 | 山东福田药业有限公司 | A kind of process for purification of xylose hydrolysis fluid |
| CN109293714A (en) * | 2018-09-04 | 2019-02-01 | 成都连接流体分离科技有限公司 | A kind of refining methd of high temperature wood hydrolysis liquid |
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| CN108004351B (en) * | 2017-12-08 | 2020-09-22 | 山东福田药业有限公司 | Refining method of xylose hydrolysate |
| CN109293714A (en) * | 2018-09-04 | 2019-02-01 | 成都连接流体分离科技有限公司 | A kind of refining methd of high temperature wood hydrolysis liquid |
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