CN108409609A - Arginine electrodialysis extraction process - Google Patents
Arginine electrodialysis extraction process Download PDFInfo
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- CN108409609A CN108409609A CN201810094604.5A CN201810094604A CN108409609A CN 108409609 A CN108409609 A CN 108409609A CN 201810094604 A CN201810094604 A CN 201810094604A CN 108409609 A CN108409609 A CN 108409609A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C277/00—Preparation of guanidine or its derivatives, i.e. compounds containing the group, the singly-bound nitrogen atoms not being part of nitro or nitroso groups
- C07C277/08—Preparation of guanidine or its derivatives, i.e. compounds containing the group, the singly-bound nitrogen atoms not being part of nitro or nitroso groups of substituted guanidines
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/10—Citrulline; Arginine; Ornithine
Abstract
The invention discloses a kind of arginine electrodialysis extraction processes, belong to technical field of amino acid production.The present invention.The present invention effectively realizes arginic cleaner production using the combination of the advanced treatment process such as ceramic membrane separation, electrodialysis desalination, film concentration.The key point of the technique is to be exchanged instead of conventional ion with electrodialysis, improves product recovery rate, improves product purity, while reducing the soda acid dosage and water consume of original technique, reduces the activated carbon dosage of subsequent technique, reduce environmental pollution.
Description
Technical field
The present invention relates to technical field of amino acid production, more particularly to a kind of arginine electrodialysis extraction process.
Background technology
Traditional biological zymotechnique prepares arginine.Contain a large amount of organic and nothing in bio-fermentation process in zymotic fluid
Machine impurity, traditional rear extraction process technique do not ensure product quality, and time-consuming.The prior art uses when extracting
Plate-frame filtering, the time is long, yield is low, and loss is big.Traditional handicraft can only achieve 50% total recovery, and first-time qualification rate only has
97%。
Authorization Notice No. filed in 24 days December in 2013 of our company is " 103695487 B of CN ", entitled
The patent of invention of " a kind of microbial fermentation production arginine technique " is disclosed must contain essence using the culture of MQA-121 strain fermentations
The zymotic fluid of propylhomoserin, and the arginic method of separation and Extraction from the zymotic fluid.
The arginine purification mode of the patent disclosure, arginine extract yield are up to 86.3%, and product qualification rate is up to
99.9%, although the purification mode has been obtained for significant progress compared with the existing technology, however, actual production process
There are many deficiencies, such as water consumption are big, sewage yield is excessively high, and activated carbon dosage is larger, causes artificial and supplies consumption
Extraction cost is higher, and extract yield also has the space promoted in addition.
Invention content
In order to make up for the deficiencies of the prior art, the present invention provides a kind of arginine electrodialysis extraction processes.
The technical scheme is that：
A kind of arginine electrodialysis extraction process, including step：
1）Ceramic membrane filter：By the zymotic fluid of brevibacterium flavum MQA121 through ceramic membrane filter, the production bacterium in zymotic fluid is removed
And high molecular weight protein, obtain pottery cleaner liquid；The actual conditions of the ceramic membrane filter are：Ceramic membrane aperture is 120-500nm, operation
Pressure difference is 0.15-0.40MPa, crossflow velocity 3.0-3.5m/s, and it is 70-120L/ m to intend stabilized flux2.h；
2）Electrodialysis：The pottery cleaner liquid is handled by electrodialysis desalination, obtains dialysis clear liquid；The electrodialysis desalination processing
Equipment uses out-phase alloy film or homogeneous mould；
3）Nanofiltration；The dialysis clear liquid uses latus rectum to be handled for the nano-filtration membrane equipment of 800 molecular weight, and it is 5- to obtain arginine content
7% nanofiltration concentrate；The temperature of nanofiltration processing procedure is less than 50 DEG C；
4）Activated carbon decolorizing：The nanofiltration concentrate is warming up to 55-65 DEG C, is added with stirring activated carbon decolorizing, heat preservation decoloration
40-60min is handled, decoloration clear liquid is obtained；
5）Reverse osmosis concentration：The decoloration clear liquid is concentrated into arginine content after 12%-15%, to obtain reverse osmosis through reverse osmosis membrane
Saturating concentrate；
6）Anion exchange；The reverse osmosis concentrated liquid is cloudy by alkalescent with the flow velocity of 0.8-1.2 times of resin volume/hour
Ion exchange resin obtains resin anion (R.A.) and exchanges liquid；
7）Condensing crystallizing：Resin anion (R.A.) exchange liquid is concentrated, the concentration that arginine content is 80%-85% is concentrated into
Liquid；The concentrate is cooled to room temperature and the growing the grain 8h under the conditions of 5-10 DEG C；Filter to obtain arginine crystal wet product；
8）It is dry：Arginine crystal wet product is dried, the arginine product of purifying is obtained.
Preferably, step 2）In, the actual conditions of electrodialysis desalination processing are：Electrodialysis plant working voltage
20v, curent change range 1-20A, electrode solution are that the ammonium sulfate solution pH of 1.5%-2.5% maintains 6.0-7.0.
Preferably, step 4）In, the dosage of activated carbon is the 0.8%-1.2% of the nanofiltration concentrate quality.
Preferably, step 5）In, during reverse osmosis concentration, temperature is less than 60 DEG C.
Preferably, step 7）Specifically, the resin anion (R.A.), which is exchanged liquid, sucks condensing crystallizing tank ,-
0.08Mpa, 55-65 DEG C, resin anion (R.A.) exchange liquid concentrated under stirring condition.
Preferably, step 8）Middle arginine crystal wet product is dried through bipyramid, in vacuum degree -0.08Mpa, temperature 80
It is dried under the conditions of DEG C and obtains within 5 hours 0.5% dry product below of water content.
Preferably, the preparation method of the zymotic fluid of the brevibacterium flavum MQA121 is as follows:
1）Inclined-plane culture：By the slant medium of MQA121 strains access sterilizing, is cultivated at 32 DEG C, obtain inclined-plane bacterium within 32 hours
Kind；
2）Shaking flask strain：Cultured slant strains access 32 DEG C of cultures of shake-flask seed culture medium are obtained into shaking flask in 22-32 hours
Seed liquor；
3）First order seed culture：By the primary-seed medium of cultured shake-flask seed liquid access sterilizing, 32 DEG C of culture 22-28
Hour obtains level-one kind liquid；
4）Secondary seed culture：32 DEG C of culture 4-8 of secondary seed medium that the access of cultured primary seed solution was sterilized
Hour obtains secondary seed solution；
5）Cultured secondary seed solution is inoculated into the fermentation medium of sterilizing, fermentation process is added by weight percentage
3% glucose and corn steep liquor 3.0-5.0%, (NH4)2SO41.5-3.5%、KH2PO40.3-0.8%、K2HPO4·3H2O0.3-0.6%,
It is passed through sterile wind, using ammonium hydroxide control ph 6.8, under the conditions of 32 DEG C, product containing arginine is obtained by culture in 65-70 hours
Zymotic fluid.
Further, by weight percentage, the slant medium is：Glucose 2.5-4.0%, (NH4)2SO41.0-
2.5%、KH2PO40.3-0.8%、K2HPO4·3H2O0.3-0.8%、MgSO4·7H2O0.05-0.1%、 FeSO4·7H2O
0.002-0.004%、 MnSO4·H2O0.002-0.010%, agar powder 2.0%, pH6.4 ~ 7.0.
Preferably, by weight percentage, the shake-flask seed culture medium, primary-seed medium, secondary seed
Culture medium and fermentation medium are：Initial sugar concentration 12.5%, urea 0.3%, corn steep liquor CSL4.0%,（NH4）SO45.0%。
MQA121 bacterial strains are in China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation, address：Beijing
The institute 3 of city Chaoyang District North Star West Road 1, postcode：100101, deposit number is：CGMCC No.8392, the Latin of strain
Title isBrevibacteriumfLavum, join the microorganism of evidence（Strain）：MQA121, preservation date are on October 25th, 2013.
The bacterial strain acid producing ability is strong, saccharic acid conversion ratio is high, performance is stablized.
Beneficial effects of the present invention are：
1, product yield and product quality are improved by the combination of new extraction and purification process.Arginic disposable extract yield
Reach 90%, purity is up to 99%；
2, present invention electrodialysis greatly reduces sewage yield instead of conventional ion exchange process.Prior art uses
Ion exchange carries out product separation, and sewage yield is larger, and environmental protection pressure is higher.Product per ton consumes about 150 tons of fresh water amount,
Generate about 130 tons of sewage.And after using electrodialysis process, sewage yield declines 50% or more, and product per ton consumes fresh water amount
About 80 tons, generate about 50 tons of sewage.
3, present invention electrodialysis has saved cost of labor and supplies consumption instead of conventional ion exchange process, reduces
Production cost.Ion-exchange process is artificial operation and control at present, and it is 10 people or so which, which needs to configure personnel, is extracted into
This about 8000 yuan/ton, and after using upgrading process, automation control may be used in electrodialysis process, can manually be reduced to 3
People, and acid, alkali, activated carbon dosage are effectively reduced, extraction cost is about 5000 yuan/ton, reduces by 1/3 or so.
4, the zymotechnique of MQA121 bacterial strains is finely adjusted in the present invention（It it is December 24 in 2013 relative to the applying date
Day, Authorization Notice No. be 103695487 B of CN, a kind of entitled microbial fermentation produce arginine technique invention it is special
Technological condition for fermentation in profit）, significantly increase arginic yield.
Specific implementation mode
Embodiment 1：
The present embodiment bacterial strain uses therefor is MQA-121, and MQA121 bacterial strains are commonly micro- in China Committee for Culture Collection of Microorganisms
Bio-Centers preservation, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, postcode：100101, deposit number is：CGMCC
The Latin title of No.8392, strain isBrevibacteriumfLavum, join the microorganism of evidence（Strain）：MQA121, preservation day
Phase is on October 25th, 2013.The bacterial strain acid producing ability is strong, saccharic acid conversion ratio is high, performance is stablized.
The preparation of the zymotic fluid of brevibacterium flavum MQA121 includes following step：
（1）Inclined-plane culture：By the slant medium of MQA121 strains access sterilizing（It is formulated glucose 2.5-4.0%, (NH4)2SO41.0-2.5%、KH2PO40.3-0.8%、K2HPO4·3H2O0.3-0.8%、MgSO4·7H2O0.05-0.1%、FeSO4·7H2O
0.002-0.004%、MnSO4·H2O0.002-0.010%, agar powder 2.0%, pH6.4 ~ 7.0.）, it is cultivated at 32 DEG C, 32 hours
To slant strains；
（2）Shaking flask strain：The access shaking flask sterilizing 32 DEG C of cultures of seed culture medium of cultured slant strains are obtained for 22-32 hours
Shake-flask seed liquid；
（3）First order seed culture：By the primary-seed medium of cultured shake-flask seed liquid access sterilizing, 32 DEG C of culture 22-
Obtain level-one kind liquid within 28 hours；
（4）Secondary seed culture：32 DEG C of culture 4-8 of secondary seed medium that the access of cultured primary seed solution was sterilized
Hour obtains secondary seed solution；
（5）Cultured secondary seed solution is inoculated into the fermentation medium of sterilizing, it is 3% grape that fermentation process, which adds content,
Sugar and nutriment（Corn steep liquor 3.0-5.0%, (NH4)2SO41.5-3.5%、KH2PO40.3-0.8%、K2HPO4·3H2O0.3-
0.6%）, it is passed through sterile wind, using ammonium hydroxide（1:1 volumetric concentration）Control ph 6.8, under the conditions of 32 DEG C, by 65-70 hours
Culture obtains the zymotic fluid of the product containing arginine.
Shake-flask seed culture medium, primary-seed medium, secondary seed medium and fermentative medium formula are：Just sugar
Concentration 12.5%, urea 0.3%, corn steep liquor CSL4.0%,（NH4）SO45.0%。
The broth extraction of brevibacterium flavum MQA121 is refined to be included the following steps：（1）Zymotic fluid ceramic membrane filter；（2）
Electrodialysis；（3）Nanofiltration；（4）Activated carbon decolorizing；（5）Reverse osmosis concentration；（6）Anion exchange；（7）Concentration, crystallization；（8）Centrifugation
It is dry.
Specially：
（1）Fermentation liquor pretreatment removes production bacterium and high molecular weight protein in zymotic fluid using ceramic membrane equipment.Ceramic membrane aperture
120-500nm operates pressure difference 0.15-0.40MPa, crossflow velocity 3.0-3.5m/s, intends stabilized flux 70-120L/m2.h。
Zymotic fluid is directly over ceramic membrane circulating filtration, and process uses recirculated water for cycle feed liquid cooling, recycles feed liquid temperature
Degree control is at 65 DEG C hereinafter, reverse osmosis water, which is added, in concentrate washes out remaining product.Filtration time is 12 hours, and cleaner liquid contains
Amount is 60-80g/L, and filtrate clarifies, is transparent, and pretreatment yield is 85%.After ceramic membrane, clear liquid bacteria containing amount ＜ 0.2%.
（2）Electrodialysis is by step（1）In obtained cleaner liquid pass through electrodialysis desalination, obtain infiltration clear liquid, clear liquid conductance
Rate 500-1000 μ S/cm, supernatant volume are the 90-100% of original material liquid volume；
（3）Nanofiltration is by step（2）In dialysis clear liquid pass through latus rectum be 800 molecular weight nano-filtration membrane equipment cycle decoloration, process
It is less than 50 DEG C using circulating water cooling control loop feed temperature, is less than with reverse osmosis water cleaning concentrate to product content
0.2%, it is 80% or more to obtain light transmission, the nanofiltration concentrate that content is 6%；
（4）Activated carbon decolorizing is by step（3）Nanofiltration clear liquid is warming up to 60 DEG C, and it is de- that 1% activated carbon is added under 120rpm stirrings
Color keeps the temperature 45 minutes, and removing activated carbon through decarburizing machine after clear liquid light transmittance reaches 93% or more obtains decoloration clear liquid,
（5）It is reverse osmosis by step（4）Decoloration clear liquid passes through reverse-osmosis circulating, low with circulating water cooling control loop feed temperature
In 60 DEG C, concentration liquid hold-up obtains reverse osmosis concentrated liquid after reaching 12-15%；
（6）Anion exchange resin step（5）Reverse osmosis concentrated liquid is flowed through with the flow velocity of 1 times of resin volume/hour by weak base
Property resin anion (R.A.) obtain content be 13% light transmittance 99.5% resin anion (R.A.) exchange liquid.
（7）Concentration is crystallized step（6）Anion exchange liquid sucks condensing crystallizing tank, in -0.08Mpa, feed temperature control
System reaches 85% in 60 DEG C, speed of agitator for anion exchange liquid is concentrated into feed liquid content under conditions of 120rpm, reduces rotating speed
To 80rpm, feed liquid is cooled to 5-10 DEG C of growing the grain 8 hours with -5 DEG C of ice water.
（8）Finished product is by step（7）Support good brilliant crystal solution with the full-automatic discharging centrifuge of 2500rpm rotating speeds will crystallization with
Mother liquor detaches, and is crystallized to obtain wet product with 75% alcohol washes, wet product is dried through bipyramid in vacuum degree -0.08Mpa, 80 DEG C of temperature
Under the conditions of dry and obtain within 5 hours 0.5% dry product below of water content, dry product is 22 DEG C -28 DEG C of temperature in environment, humidity≤50%,
Finished product is packed to obtain under aseptic condition.
It is an advantage of the current invention that replacing traditional ionic energy transfer technology using electrodialysis desalination technology, reduce
Soda acid dosage and water consumption reduce activated carbon dosage and sewage yield, improve product quality.
Preparation method through the invention, sewage yield reduce 50%, and activated carbon dosage reduces 20%, soda acid dosage drop
Low 60%.
Using above-mentioned processing means, finished product total recovery for zymotic fluid can reach 93.2% or more（Relative to removing bacterium
The net arginine content of body calculates）, finished product first-time qualification rate reaches 100%, and pilot product is through Accessories during Binzhou Administration of Quality and Technology Supervision
Tripartite examines, and indices meet National Standard of the People's Republic of China, national food safety standard, food additives, L- essences
28306-2012 standards of propylhomoserin GB, main performance index refer to table 1：
Table 1
Comparative example 1
Comparative example 1 is identical with the preparation method of zymotic fluid of 1 brevibacterium flavum MQA121 of embodiment.
In this comparative example, the broth extraction process for purification of brevibacterium flavum MQA121 is in December, 2013 according to the applying date
24, Authorization Notice No. was " 103695487 B of CN ", entitled " a kind of microbial fermentation produce arginine technique "
The disclosed arginic method of separation and Extraction from the zymotic fluid of patent of invention carries out arginic Hydrolysis kinetics.
The arginic disposable extract yield of this comparative example is 86.1%, and arginic purity is 96%, the present embodiment separation
Generated waste water is 130 ton/ton arginine in purification process.
As it can be seen that the present invention, compared with comparative example, arginic disposable extract yield improves 4%, and purity also carries significantly
It rises, in addition, generated sewage significantly reduces, greatly reduces environmental pressure, reduce the consumed energy of sewage disposal.
Claims (9)
1. a kind of arginine electrodialysis extraction process, which is characterized in that including step：
1）Ceramic membrane filter：By the zymotic fluid of brevibacterium flavum MQA121 through ceramic membrane filter, the production bacterium in zymotic fluid is removed
And high molecular weight protein, obtain pottery cleaner liquid；The actual conditions of the ceramic membrane filter are：Ceramic membrane aperture is 120-500nm, operation
Pressure difference is 0.15-0.40MPa, crossflow velocity 3.0-3.5m/s, and it is 70-120L/ m to intend stabilized flux2.h；
2）Electrodialysis：The pottery cleaner liquid is handled by electrodialysis desalination, obtains dialysis clear liquid；The electrodialysis desalination processing
Equipment uses out-phase alloy film or homogeneous mould；
3）Nanofiltration；The dialysis clear liquid uses latus rectum to be handled for the nano-filtration membrane equipment of 800 molecular weight, and it is 5- to obtain arginine content
7% nanofiltration concentrate；The temperature of nanofiltration processing procedure is less than 50 DEG C；
4）Activated carbon decolorizing：The nanofiltration concentrate is warming up to 55-65 DEG C, is added with stirring activated carbon decolorizing, heat preservation decoloration
40-60min is handled, decoloration clear liquid is obtained；
5）Reverse osmosis concentration：The decoloration clear liquid is concentrated into arginine content after 12%-15%, to obtain reverse osmosis through reverse osmosis membrane
Saturating concentrate；
6）Anion exchange；The reverse osmosis concentrated liquid is cloudy by alkalescent with the flow velocity of 0.8-1.2 times of resin volume/hour
Ion exchange resin obtains resin anion (R.A.) and exchanges liquid；
7）Condensing crystallizing：Resin anion (R.A.) exchange liquid is concentrated, the concentration that arginine content is 80%-85% is concentrated into
Liquid；The concentrate is cooled to room temperature and the growing the grain 8h under the conditions of 5-10 DEG C；Filter to obtain arginine crystal wet product；
8）It is dry：Arginine crystal wet product is dried, the arginine product of purifying is obtained.
2. arginine electrodialysis extraction process as described in claim 1, it is characterised in that：Step 2）In, electrodialysis desalination processing
Actual conditions be：Electrodialysis plant working voltage 20v, curent change range 1-20A, electrode solution are the sulfuric acid of 1.5%-2.5%
Aqueous ammonium pH maintains 6.0-7.0.
3. arginine electrodialysis extraction process as claimed in claim 1 or 2, it is characterised in that：Step 4）In, the dosage of activated carbon
For the 0.8%-1.2% of the nanofiltration concentrate quality.
4. arginine electrodialysis extraction process as claimed in claim 1 or 2, it is characterised in that：Step 5）In, reverse osmosis concentration
In the process, temperature is less than 60 DEG C.
5. arginine electrodialysis extraction process as claimed in claim 1 or 2, it is characterised in that：Step 7）Specifically, by described the moon
Ion exchange resin exchanges liquid and sucks condensing crystallizing tank, and resin anion (R.A.) is exchanged liquid under -0.08Mpa, 55-65 DEG C, stirring condition
It is concentrated.
6. arginine electrodialysis extraction process as claimed in claim 1 or 2, it is characterised in that：Step 8）Middle arginine crystal is wet
Product are dried through bipyramid, under the conditions of vacuum degree -0.08Mpa, 80 DEG C of temperature drying it is below dry to obtain within 5 hours water content 0.5%
Product.
7. arginine electrodialysis extraction process as claimed in claim 1 or 2, which is characterized in that the brevibacterium flavum MQA121
Zymotic fluid preparation method it is as follows:
1）Inclined-plane culture：By the slant medium of MQA121 strains access sterilizing, is cultivated at 32 DEG C, obtain inclined-plane bacterium within 32 hours
Kind；
2）Shaking flask strain：Cultured slant strains access 32 DEG C of cultures of shake-flask seed culture medium are obtained into shaking flask in 22-32 hours
Seed liquor；
3）First order seed culture：By the primary-seed medium of cultured shake-flask seed liquid access sterilizing, 32 DEG C of culture 22-28
Hour obtains level-one kind liquid；
4）Secondary seed culture：32 DEG C of culture 4-8 of secondary seed medium that the access of cultured primary seed solution was sterilized
Hour obtains secondary seed solution；
5）Cultured secondary seed solution is inoculated into the fermentation medium of sterilizing, fermentation process is added by weight percentage
3% glucose and corn steep liquor 3.0-5.0%, (NH4)2SO41.5-3.5%、KH2PO40.3-0.8%、K2HPO4·3H2O0.3-0.6%,
It is passed through sterile wind, using ammonium hydroxide control ph 6.8, under the conditions of 32 DEG C, product containing arginine is obtained by culture in 65-70 hours
Zymotic fluid.
8. arginine electrodialysis extraction process as claimed in claim 7, which is characterized in that by weight percentage, the inclined-plane training
Foster base is：Glucose 2.5-4.0%, (NH4)2SO41.0-2.5%、KH2PO40.3-0.8%、K2HPO4·3H2O0.3-0.8%、
MgSO4·7H2O0.05-0.1%、FeSO4·7H2O 0.002-0.004%、MnSO4·H2O0.002-0.010%, agar powder
2.0%, pH6.4 ~ 7.0.
9. arginine electrodialysis extraction process as claimed in claim 7, which is characterized in that by weight percentage, the shaking flask kind
Sub- culture medium, primary-seed medium, secondary seed medium and fermentation medium are：Initial sugar concentration 12.5%, urea
0.3%, corn steep liquor CSL4.0%,（NH4）SO45.0%。
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| CN110169467A (en) * | 2019-05-15 | 2019-08-27 | 安徽省万清堂生物科技有限公司 | Functional health tea and preparation method thereof |
| CN110169467B (en) * | 2019-05-15 | 2021-07-02 | 安徽省万清堂生物科技有限公司 | Functional health tea and preparation method thereof |
| CN112657339A (en) * | 2019-10-15 | 2021-04-16 | 中国石油化工股份有限公司 | Electrodialysis device, electrodialysis system, and method for purifying glycolic acid raw material |
| CN111718287A (en) * | 2020-06-12 | 2020-09-29 | 吉林大学 | Electrodialysis extraction method of N-acetyl-L-cysteine |
| CN111718287B (en) * | 2020-06-12 | 2022-02-18 | 吉林大学 | Electrodialysis extraction method of N-acetyl-L-cysteine |
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Application publication date: 20180817 |