CN108893510A - A kind of compounding method of the fructose syrup made of plant amylum hydrolysis and isomerization - Google Patents
A kind of compounding method of the fructose syrup made of plant amylum hydrolysis and isomerization Download PDFInfo
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- CN108893510A CN108893510A CN201810807210.XA CN201810807210A CN108893510A CN 108893510 A CN108893510 A CN 108893510A CN 201810807210 A CN201810807210 A CN 201810807210A CN 108893510 A CN108893510 A CN 108893510A
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- plant amylum
- hydrolysis
- isomerization
- fructose syrup
- saccharification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
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- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
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- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K11/00—Fructose
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Abstract
The invention discloses a kind of compounding methods of fructose syrup made of plant amylum hydrolysis and isomerization, include the following steps：S1, plant amylum hydrolysis；S2, saccharification；S3, isomerization；The step of S4, evaporation and concentration.It is energy saving present invention eliminates the process of pre-concentration, save cost；It is filtered using organic film, cooperates subsequent carbon decoloring active twice, improve decolorizing effect；Strict control reaction condition, the product color of preparation, in good taste, product quality height and stabilization.
Description
Technical field
The present invention relates to fruit grape slurry technical fields, more particularly to a kind of fruit made of plant amylum hydrolysis and isomerization
The compounding method of glucose slurry.
Background technique
Fructose syrup is that the starch sugar made of plant amylum hydrolysis and isomerization is brilliant, has peculiar flavour, is a kind of heavy
The sweetener wanted.Because its composition is mainly fructose and glucose, therefore is known as " fructose syrup ".Traditional mode of production fructose syrup
It is that method is, is configured to the starch amylase to be added after starch milk and liquefy through high-temperature injection, add glucose saccharifying enzymatic conversion
For glucose, active carbon is added after vacuum drum or plate-frame filtering are except deproteinized and once decolourizes, is removed by primary ions exchange
It is miscellaneous, after pre-concentration cross immobilized isomerase isomery, then through active carbon secondary decolourization, Two-step ion-exchanging, be concentrated by evaporation to obtain fruit Portugal
Syrup, this method production cost and energy consumption are higher, we have devised a kind of by plant amylum hydrolysis and isomerization system thus
At fructose syrup solve problem above.
Summary of the invention
The purpose of the present invention is to solve disadvantages existing in the prior art, and the one kind proposed is hydrolyzed by plant amylum
With the compounding method of fructose syrup made of isomerization.
To achieve the goals above, present invention employs following technical solutions：
A kind of compounding method of the fructose syrup made of plant amylum hydrolysis and isomerization, includes the following steps：
S1, plant amylum hydrolysis：Plant amylum is put into container, and amylase is added, is warming up to 100-120 DEG C, and stir
10-30 minutes, so that plant amylum is carried out sufficient hydrolysis, obtain liquefaction products through High-temperature Liquefaction after；
S2, saccharification：Slagging-off is filtered to liquefaction products obtained in S1, and glucolase is added and carries out saccharification reaction, to sugar
Product after change successively after organic membrane filter, first time ion exchange and the first decolorization filtering, obtains final saccharification and produces
Object；
S3, isomerization：Glucose isomerase is added into saccharification product obtained in S2, and part glucose is made to be converted into fructose,
Obtain mixed liquor A；
S4 is concentrated by evaporation：Mixed liquor A obtained in S3 is successively subjected to second of ion exchange and second of decolorization filtering, is obtained
To mixed liquid B, concentration is evaporated to mixed liquid B, obtains fruit glucose syrup.
Preferably, it in the saccharifying of the S2, needs to be added dilute hydrochloric acid solution and arrives the pH value adjustment of liquefaction products
5.1-5.6。
Preferably, the saccharificatinn period of the S2 is 18-22h.
Preferably, first time ion exchange is exchanged by ion column in the S2, removes protein and other are soluble
Nitrogenous object, each heavy metal species, faintly acid organic composition object and inorganic impurity.
Preferably, in the S4 second ion exchange be mixed liquor A is passed through equipped with anion and cation exchange resin from
Sub- exchange column makes the conductivity of the fructose syrup to discharge from ion exchange column less than 5 μ s/cm and PH value is controlled in 3.5-
4.5。
Preferably, the first time decoloration in the S2 and S4 and the second decoloration are using active carbon decoloring 40-60 minutes.
Beneficial effects of the present invention are：The process for eliminating pre-concentration, it is energy saving, save cost；In saccharification to molten
Liquid carries out the adjusting of pH value, convenient for the control and decoloration of subsequent pH value；It is filtered using organic film, cooperation is subsequent to live twice
Property carbon decoloring, good decolorizing effect；Strict control reaction condition, the product color of preparation, in good taste, product quality height and stabilization.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.
Embodiment one
A kind of compounding method of the fructose syrup made of plant amylum hydrolysis and isomerization, includes the following steps：
S1, plant amylum hydrolysis：Plant amylum is put into container, and amylase is added, is warming up to 100 DEG C, and stir 10 points
Clock makes plant amylum carry out sufficient hydrolysis, obtains liquefaction products through High-temperature Liquefaction after；
S2, saccharification：Slagging-off is filtered to liquefaction products obtained in S1, and glucolase is added and carries out saccharification reaction, is saccharified
Time is 18h, to the product after saccharification successively after organic membrane filter, first time ion exchange and the first decolorization filtering, is obtained
To final saccharification product, when decoloration, is used active carbon decoloring 40 minutes；
S3, isomerization：Glucose isomerase is added into saccharification product obtained in S2, and part glucose is made to be converted into fructose,
Obtain mixed liquor A；
S4 is concentrated by evaporation：Mixed liquor A obtained in S3 is successively subjected to second of ion exchange and second of decolorization filtering, is obtained
To mixed liquid B, using active carbon decoloring 40 minutes when decoloration, concentration is evaporated to mixed liquid B, obtains fruit glucose syrup.
Embodiment two
A kind of compounding method of the fructose syrup made of plant amylum hydrolysis and isomerization, includes the following steps：
S1, plant amylum hydrolysis：Plant amylum is put into container, and amylase is added, is warming up to 105 DEG C, and stir 15 points
Clock makes plant amylum carry out sufficient hydrolysis, obtains liquefaction products through High-temperature Liquefaction after；
S2, saccharification：Slagging-off is filtered to liquefaction products obtained in S1, and glucolase is added and carries out saccharification reaction, is saccharified
Time is 19h, to the product after saccharification successively after organic membrane filter, first time ion exchange and the first decolorization filtering, is obtained
To final saccharification product, when decoloration, is used active carbon decoloring 45 minutes；
S3, isomerization：Glucose isomerase is added into saccharification product obtained in S2, and part glucose is made to be converted into fructose,
Obtain mixed liquor A；
S4 is concentrated by evaporation：Mixed liquor A obtained in S3 is successively subjected to second of ion exchange and second of decolorization filtering, is obtained
To mixed liquid B, using active carbon decoloring 45 minutes when decoloration, concentration is evaporated to mixed liquid B, obtains fruit glucose syrup.
Embodiment three
A kind of compounding method of the fructose syrup made of plant amylum hydrolysis and isomerization, includes the following steps：
S1, plant amylum hydrolysis：Plant amylum is put into container, and amylase is added, is warming up to 115 DEG C, and stir 25 points
Clock makes plant amylum carry out sufficient hydrolysis, obtains liquefaction products through High-temperature Liquefaction after；
S2, saccharification：Slagging-off is filtered to liquefaction products obtained in S1, and glucolase is added and carries out saccharification reaction, is saccharified
Time is 21h, to the product after saccharification successively after organic membrane filter, first time ion exchange and the first decolorization filtering, is obtained
To final saccharification product, when decoloration, is used active carbon decoloring 50 minutes；
S3, isomerization：Glucose isomerase is added into saccharification product obtained in S2, and part glucose is made to be converted into fructose,
Obtain mixed liquor A；
S4 is concentrated by evaporation：Mixed liquor A obtained in S3 is successively subjected to second of ion exchange and second of decolorization filtering, is obtained
To mixed liquid B, using active carbon decoloring 50 minutes when decoloration, concentration is evaporated to mixed liquid B, obtains fruit glucose syrup.
Example IV
A kind of compounding method of the fructose syrup made of plant amylum hydrolysis and isomerization, includes the following steps：
S1, plant amylum hydrolysis：Plant amylum is put into container, and amylase is added, is warming up to 120 DEG C, and stir 30 points
Clock makes plant amylum carry out sufficient hydrolysis, obtains liquefaction products through High-temperature Liquefaction after；
S2, saccharification：Slagging-off is filtered to liquefaction products obtained in S1, and glucolase is added and carries out saccharification reaction, is saccharified
Time is 22h, to the product after saccharification successively after organic membrane filter, first time ion exchange and the first decolorization filtering, is obtained
To final saccharification product, when decoloration, is used active carbon decoloring 60 minutes；
S3, isomerization：Glucose isomerase is added into saccharification product obtained in S2, and part glucose is made to be converted into fructose,
Obtain mixed liquor A；
S4 is concentrated by evaporation：Mixed liquor A obtained in S3 is successively subjected to second of ion exchange and second of decolorization filtering, is obtained
To mixed liquid B, using active carbon decoloring 60 minutes when decoloration, concentration is evaporated to mixed liquid B, obtains fruit glucose syrup.
In above-described embodiment, embodiment three is best real-time proposals, i.e. plant amylum water that amylase is added under 115 degree
Solution reaction is most abundant, the saccharification result of 21 hours of saccharification is best, using 50 minutes pigment Adsorptions of active carbon decoloring
Most completely, the glucose syrup mouthfeel of preparation is also best.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of compounding method of the fructose syrup made of plant amylum hydrolysis and isomerization, which is characterized in that including following
Step：
S1, plant amylum hydrolysis：Plant amylum is put into container, and amylase is added, is warming up to 100-120 DEG C, and stir
10-30 minutes, so that plant amylum is carried out sufficient hydrolysis, obtain liquefaction products through High-temperature Liquefaction after；
S2, saccharification：Slagging-off is filtered to liquefaction products obtained in S1, and glucolase is added and carries out saccharification reaction, to sugar
Product after change successively after organic membrane filter, first time ion exchange and the first decolorization filtering, obtains final saccharification and produces
Object；
S3, isomerization：Glucose isomerase is added into saccharification product obtained in S2, and part glucose is made to be converted into fructose,
Obtain mixed liquor A；
S4 is concentrated by evaporation：Mixed liquor A obtained in S3 is successively subjected to second of ion exchange and second of decolorization filtering, is obtained
To mixed liquid B, concentration is evaporated to mixed liquid B, obtains fruit glucose syrup.
2. a kind of compounding method of fructose syrup made of plant amylum hydrolysis and isomerization according to claim 1,
It is characterized in that, needing to be added dilute hydrochloric acid solution for the pH value adjustment of liquefaction products to 5.1- in the saccharifying of the S2
5.6。
3. a kind of compounding method of fructose syrup made of plant amylum hydrolysis and isomerization according to claim 1,
It is characterized in that, the saccharificatinn period of the S2 is 18-22h.
4. a kind of compounding method of fructose syrup made of plant amylum hydrolysis and isomerization according to claim 1,
It is characterized in that, first time ion exchange is exchanged by ion column in the S2, protein and other soluble nitrogenous are removed
Object, each heavy metal species, faintly acid organic composition object and inorganic impurity.
5. a kind of compounding method of fructose syrup made of plant amylum hydrolysis and isomerization according to claim 1,
It is characterized in that, second of ion exchange is that mixed liquor A is passed through the ion equipped with anion and cation exchange resin to hand in the S4
Column is changed, makes the conductivity of the fructose syrup to discharge from ion exchange column less than 5 μ s/cm and PH value is controlled in 3.5- 4.5.
6. a kind of compounding method of fructose syrup made of plant amylum hydrolysis and isomerization according to claim 1,
It is characterized in that, the first time decoloration and the second decoloration in the S2 and S4 are using active carbon decoloring 40-60 minutes.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810807210.XA CN108893510A (en) | 2018-07-21 | 2018-07-21 | A kind of compounding method of the fructose syrup made of plant amylum hydrolysis and isomerization |
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| CN201810807210.XA CN108893510A (en) | 2018-07-21 | 2018-07-21 | A kind of compounding method of the fructose syrup made of plant amylum hydrolysis and isomerization |
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| CN108893510A true CN108893510A (en) | 2018-11-27 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110643657A (en) * | 2019-09-11 | 2020-01-03 | 保龄宝生物股份有限公司 | Production method of high fructose corn syrup without acidophilic thermophilic bacteria |
| CN116064972A (en) * | 2022-12-17 | 2023-05-05 | 东台品青生物科技有限公司 | Method for preparing high fructose corn syrup from rice starch |
-
2018
- 2018-07-21 CN CN201810807210.XA patent/CN108893510A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110643657A (en) * | 2019-09-11 | 2020-01-03 | 保龄宝生物股份有限公司 | Production method of high fructose corn syrup without acidophilic thermophilic bacteria |
| CN116064972A (en) * | 2022-12-17 | 2023-05-05 | 东台品青生物科技有限公司 | Method for preparing high fructose corn syrup from rice starch |
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Application publication date: 20181127 |