CN102676605A - Method for producing xylose and L-arabinose using xylose mother liquor - Google Patents
Method for producing xylose and L-arabinose using xylose mother liquor Download PDFInfo
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- CN102676605A CN102676605A CN2012101584400A CN201210158440A CN102676605A CN 102676605 A CN102676605 A CN 102676605A CN 2012101584400 A CN2012101584400 A CN 2012101584400A CN 201210158440 A CN201210158440 A CN 201210158440A CN 102676605 A CN102676605 A CN 102676605A
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Abstract
The invention discloses a method for producing xylose and L-arabinose using xylose mother liquor, and the method is characterized by comprising the following steps of: 1, propagating yeast, using a corn steep liquor powder nutrient solution to dilute the xylose mother liquor, preparing a yeast seed liquid, and adding the yeast seed liquid into a fermentation tank; 2, preparing a fermentation liquid, firstly, adding the right amount of deionized water into a dosing tank, then adding the corn steep liquor powder, and uniformly stirring the liquid; then, adding the xylose mother liquor, moisturizing, adjusting the pH value to be 4.5-5.5, and adjusting the temperature to be 25 DEG C; 3, adding the fermentation liquid into the fermentation tank, ventilating and breeding 10-12 hours, after the concentration of the yeast rises above 1*10<8>/ml, stopping wind and fermenting 36 hours; and 4, after fermentation, performing decoloration and filtration, ion exchange, vacuum concentration, chromatographic separation, crystallization, separation, dissolution of the deionized water, recrystallization, separation, washing of the deionized water, and drying, thus obtaining highly purified xylose and the L-arabinose. By means of the method, the steam consumption can be reduced, the fermentation efficiency can be improved, the product purity can be improved, and the production cost can be reduced.
Description
Technical field
The present invention relates to the working method of wood sugar and L-arabinose, specifically, relate to a kind of method of producing wood sugar and L-arabinose with xylose mother liquid.
Background technology
Xylose mother liquid is the darker thick liquid of color that stays behind the xylose crystalline, and multiple sugar components such as wood sugar, L-arabinose, glucose, semi-lactosi, seminose are contained in the inside.Wherein: wood sugar and L-arabinose are nonfermented sugar, account for 70% of total reducing sugar, and the value of these two kinds of sugar is very high; Glucose, semi-lactosi etc. are fermentable sugars, account for 26% of total reducing sugar, and these two kinds sugar are worth very low; Other sugar component also has assorted sugar such as seminose, rhamnosyl, accounts for 4% of total reducing sugar.The total sugar content of the xylose mother liquid of different manufacturers and different lot numbers is different, and in general, the total sugar content of xylose mother liquid is between 60-75%.
Because the assorted sugared content of xylose mother liquid the inside is higher, cause wherein wood sugar and the solubleness increase of L-arabinose, cause traditional method to crystallize out.So just present, xylose mother liquid is waste liquid basically, can only sell dark reddish brown factory at a low price and produce caramel colorant, economic benefit is very low.
In the prior art; The method of from xylose mother liquid, extracting, producing wood sugar and L-arabinose has: 1, adopt simulated moving bed technology direct separation xylose mother liquid to carry out purifying; Extract wood sugar and L-arabinose; Though this method is feasible, can not continuous production and gained wood sugar and pectinose purity low (93-96%); 2, utilize yeast fermentation purifying xylose mother liquid, but because the osmotic pressure that yeast can bear is lower, when the fermentation pol is suitable (general 12 degree); Fermentable sugars is very low, so, utilize yeast fermentation again after xylose mother liquid being diluted to low concentration earlier; Vacuum-evaporation then; Concentrate the moisture of the inside, fermentation efficiency is low when actual production, energy dissipation is big for this method, the Icing Sugar purity that obtains also low (94-96.5%)
Summary of the invention
The objective of the invention is to overcome the defective of prior art, a kind of method of producing wood sugar and L-arabinose with xylose mother liquid is provided, its fermentation efficiency is high, energy consumption is low, and can produce high purity wood sugar and L-arabinose.
For addressing the above problem, the technical scheme that the present invention adopted is:
A kind of method of producing wood sugar and L-arabinose with xylose mother liquid is characterized in that: comprise the steps:
1, yeast spreads cultivation, and xylose mother liquid dilutes with 1% corn starch nutritive medium, and the pol that spreads cultivation is 10 ° of BX, and ratio spreads cultivation: laboratory stage is 1: 10, is 1: 5 after jar given birth in the entering Chinese; Producing an amount of pol by this step is 8.7% yeast starter liquid, adds in the fermentor tank;
2, preparation fermented liquid, the first appropriate amount of deionized water that in material-compound tank, adds, general load weighted 1.5% corn starch joins in the material-compound tank again, and stirs; Then, in material-compound tank, add load weighted 70% xylose mother liquid, and moisturizing, transfer pH value to 4.5～5.5, temperature adjustment degree to 25 ℃;
3, fermentation, the fermented liquid that step (2) is prepared adds in the fermentor tank, and breeding 10-12 hour of ventilating treats that yeast concn rises to 1 * 10
8Individual/more than the ml, blowing-out fermentation 36 hours; The index of maturing fermentation liquid is: glucose accounts for total reducing sugar≤1.0%; Semi-lactosi accounts for total reducing sugar≤3.5%;
4, after the fermentation ends, pass through earlier decolorization filtering, IX, vacuum concentration, chromatographic separation, crystallization, separation successively; Use deionized water dissolving then, temperature is 65 ℃; Pass through recrystallization, separation, deionized water wash, drying more successively, obtaining wood sugar and purity that purity is 98.5-99.5% at last is the L-arabinose of 99.0%-99.6%.
Compared with prior art; The present invention adopts a kind of anti-yeast than high osmotic pressure (total pol 30-32) to fermenting in the xylose mother liquid, removes low value sugar such as glucose in the xylose mother liquid, semi-lactosi, improves the purity of wood sugar, pectinose high value sugar; Then; Through decolouring, IX, vacuum concentration, chromatographic separation, crystallization, separation, deionized water dissolving, recrystallization, separation, deionized water wash, drying obtain high purity wood sugar and L-arabinose at last again.In the whole process of production, steam consumption can be saved 0.77 times, and efficient improves 2.6 times, and the wood sugar that obtains and L-arabinose purity are than existing technology raising 3-5%; In addition, the present invention utilizes the corn starch as the required nitrogenous source of yeast growth, because lower, the wide material sources of corn starch price; Doing substratum with peptone, Carnis Bovis seu Bubali cream and yeast extractive substance etc. commonly used compares; Cost savings 90%, usefulness is close, can reduce great amount of cost.
Below in conjunction with embodiment the present invention is done further description.
Embodiment
With 20 tons of fermentor tanks is example, and the method for osmophilic strain yeast fermentation xylose mother liquid of the present invention being produced wood sugar and L-arabinose further describes.20 tons of fermentor tank chargings should be feeded 17 tons than by 85% calculating.
A kind of method of producing wood sugar and L-arabinose with xylose mother liquid comprises the steps:
1, yeast spreads cultivation.Xylose mother liquid dilutes with 1% corn starch nutritive medium, and the pol that spreads cultivation is 10 ° of BX, and ratio spreads cultivation: laboratory stage is 1: 10, is 1: 5 after jar given birth in the entering Chinese.Producing pol by this step is 3.4 tons of 8.7% yeast starter liquid, adds in the fermentor tank.
2, preparation fermented liquid.Earlier in material-compound tank, add appropriate amount of deionized water, the corn starch that accurately takes by weighing 102 kilogram 1.5% again joins in the material-compound tank, and stirs; Then, in material-compound tank, add 6.8 tons of 70% xylose mother liquids, pH value to 4.5～5.5 are transferred in moisturizing to 13.6 ton, temperature adjustment degree to 25 ℃.
3, fermentation.The fermented liquid that step 2 is prepared adds in the fermentor tank, and breeding 10-12 hour of ventilating treats that yeast concn rises to 1 * 10
8Individual/more than the ml, blowing-out fermentation 36 hours.Maturing fermentation liquid index is: glucose accounts for total reducing sugar≤1.0%; Semi-lactosi accounts for total reducing sugar≤3.5%.
4, after the fermentation ends, pass through earlier decolorization filtering, IX, vacuum concentration, chromatographic separation, crystallization, separation successively; Use deionized water dissolving then, temperature is 65 ℃; Pass through recrystallization, separation, deionized water wash, drying more successively, obtaining wood sugar and purity that purity is 98.5-99.5% at last is the L-arabinose of 99.0%-99.6%.
Technologies such as the decolorization filtering described in the above-mentioned step 4, IX, vacuum concentration, chromatographic separation, crystallization, separation, deionized water dissolving, recrystallization, separation, deionized water wash, drying are technique means commonly used in the prior art, repeat no more at this.
Claims (1)
1. a method of producing wood sugar and L-arabinose with xylose mother liquid is characterized in that: comprise the steps:
(1) yeast spreads cultivation, and xylose mother liquid dilutes with 1% corn starch nutritive medium, and the pol that spreads cultivation is 10 ° of BX, and ratio spreads cultivation: laboratory stage is 1: 10, is 1: 5 after jar given birth in the entering Chinese; Producing an amount of pol by this step is 8.7% yeast starter liquid, adds in the fermentor tank;
(2) preparation fermented liquid, the first appropriate amount of deionized water that in material-compound tank, adds, general load weighted 1.5% corn starch joins in the material-compound tank again, and stirs; Then, in material-compound tank, add load weighted 70% xylose mother liquid, and moisturizing, transfer pH value to 4.5～5.5, temperature adjustment degree to 25 ℃;
(3) fermentation, the fermented liquid that step (2) is prepared adds in the fermentor tank, and breeding 10-12 hour of ventilating treats that yeast concn rises to 1 * 10
8Individual/more than the ml, blowing-out fermentation 36 hours; The index of maturing fermentation liquid is: glucose accounts for total reducing sugar≤1.0%; Semi-lactosi accounts for total reducing sugar≤3.5%;
(4) after the fermentation ends, pass through earlier decolorization filtering, IX, vacuum concentration, chromatographic separation, crystallization, separation successively; Use deionized water dissolving then, temperature is 65 ℃; Pass through recrystallization, separation, deionized water wash, drying more successively, obtaining wood sugar and purity that purity is 98.5-99.5% at last is the L-arabinose of 99.0%-99.6%.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103351412A (en) * | 2013-08-02 | 2013-10-16 | 山东大学 | Method for improving yield of crystalline xylose by virtue of biological treatment |
| CN103361391A (en) * | 2013-08-02 | 2013-10-23 | 山东大学 | Method for preparing fermentation broth rich in xylose and arabinose from broadleaf wood xylose production waste liquid |
| CN108618089A (en) * | 2018-04-20 | 2018-10-09 | 中粮屯河糖业股份有限公司 | Compound sweetener and preparation method thereof |
| CN112079886A (en) * | 2020-09-17 | 2020-12-15 | 甘肃赫原生物制品有限公司 | Method for improving purity of xylose and arabinose by chromatographic separation |
| CN112094956A (en) * | 2020-09-17 | 2020-12-18 | 甘肃赫原生物制品有限公司 | Method for removing glucose by continuous fermentation of xylose mother liquor |
| CN112480185A (en) * | 2020-11-09 | 2021-03-12 | 安阳市豫鑫木糖醇科技有限公司 | Process for extracting xylose by biological method |
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| CN101555503A (en) * | 2008-04-11 | 2009-10-14 | 上海金杉生物科技有限公司 | Method for separating and extracting L-arabinose from waste wood sugar mother liquid from wood sugar production |
| CN101665523A (en) * | 2009-09-30 | 2010-03-10 | 济南圣泉集团股份有限公司 | Method for producing L-arabinose and D-xylose |
| CN101857523A (en) * | 2010-06-07 | 2010-10-13 | 禹城绿健生物技术有限公司 | Method for producing xylitol and arabitol simultaneously by utilizing xylose mother liquid |
| CN102286571A (en) * | 2011-06-16 | 2011-12-21 | 北京化工大学 | Clean and high-efficiency production process for preparing xylose and L-arabinose |
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2012
- 2012-05-11 CN CN2012101584400A patent/CN102676605A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101555503A (en) * | 2008-04-11 | 2009-10-14 | 上海金杉生物科技有限公司 | Method for separating and extracting L-arabinose from waste wood sugar mother liquid from wood sugar production |
| CN101665523A (en) * | 2009-09-30 | 2010-03-10 | 济南圣泉集团股份有限公司 | Method for producing L-arabinose and D-xylose |
| CN101857523A (en) * | 2010-06-07 | 2010-10-13 | 禹城绿健生物技术有限公司 | Method for producing xylitol and arabitol simultaneously by utilizing xylose mother liquid |
| CN102286571A (en) * | 2011-06-16 | 2011-12-21 | 北京化工大学 | Clean and high-efficiency production process for preparing xylose and L-arabinose |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103351412A (en) * | 2013-08-02 | 2013-10-16 | 山东大学 | Method for improving yield of crystalline xylose by virtue of biological treatment |
| CN103361391A (en) * | 2013-08-02 | 2013-10-23 | 山东大学 | Method for preparing fermentation broth rich in xylose and arabinose from broadleaf wood xylose production waste liquid |
| CN103351412B (en) * | 2013-08-02 | 2016-04-20 | 山东大学 | A kind of method utilizing biological treatment to improve crystalline xylose yield |
| CN108618089A (en) * | 2018-04-20 | 2018-10-09 | 中粮屯河糖业股份有限公司 | Compound sweetener and preparation method thereof |
| CN108618089B (en) * | 2018-04-20 | 2021-10-15 | 中粮糖业控股股份有限公司 | Compound sweetener and preparation method thereof |
| CN112079886A (en) * | 2020-09-17 | 2020-12-15 | 甘肃赫原生物制品有限公司 | Method for improving purity of xylose and arabinose by chromatographic separation |
| CN112094956A (en) * | 2020-09-17 | 2020-12-18 | 甘肃赫原生物制品有限公司 | Method for removing glucose by continuous fermentation of xylose mother liquor |
| CN112480185A (en) * | 2020-11-09 | 2021-03-12 | 安阳市豫鑫木糖醇科技有限公司 | Process for extracting xylose by biological method |
| CN112480185B (en) * | 2020-11-09 | 2024-02-09 | 河南豫鑫糖醇有限公司 | Process for extracting xylose by biological method |
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Application publication date: 20120919 |