CN107287258A - A kind of preparation method of the resistant dextrin containing oligoisomaltose - Google Patents
A kind of preparation method of the resistant dextrin containing oligoisomaltose Download PDFInfo
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- CN107287258A CN107287258A CN201710475026.5A CN201710475026A CN107287258A CN 107287258 A CN107287258 A CN 107287258A CN 201710475026 A CN201710475026 A CN 201710475026A CN 107287258 A CN107287258 A CN 107287258A
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- resistant dextrin
- oligoisomaltose
- enzymolysis liquid
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- 229920001353 Dextrin Polymers 0.000 title claims abstract description 31
- 239000004375 Dextrin Substances 0.000 title claims abstract description 31
- 235000019425 dextrin Nutrition 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 48
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 24
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000006243 chemical reaction Methods 0.000 claims abstract description 22
- 238000005342 ion exchange Methods 0.000 claims abstract description 19
- 239000006166 lysate Substances 0.000 claims abstract description 18
- 239000000843 powder Substances 0.000 claims abstract description 17
- 239000000047 product Substances 0.000 claims abstract description 17
- 239000012530 fluid Substances 0.000 claims abstract description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 15
- 239000008103 glucose Substances 0.000 claims abstract description 15
- 229920002774 Maltodextrin Polymers 0.000 claims abstract description 13
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 11
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 11
- 108010019077 beta-Amylase Proteins 0.000 claims abstract description 11
- 239000012043 crude product Substances 0.000 claims abstract description 11
- 239000000203 mixture Substances 0.000 claims abstract description 11
- 239000005913 Maltodextrin Substances 0.000 claims abstract description 10
- 229940035034 maltodextrin Drugs 0.000 claims abstract description 10
- 239000007787 solid Substances 0.000 claims abstract description 10
- 229930182470 glycoside Natural products 0.000 claims abstract description 9
- 150000002338 glycosides Chemical class 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 9
- 108090000637 alpha-Amylases Proteins 0.000 claims abstract description 8
- 102000004139 alpha-Amylases Human genes 0.000 claims abstract description 8
- 229940024171 alpha-amylase Drugs 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 6
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 5
- 238000000926 separation method Methods 0.000 claims abstract description 5
- 238000000855 fermentation Methods 0.000 claims abstract description 4
- 230000004151 fermentation Effects 0.000 claims abstract description 4
- 239000011347 resin Substances 0.000 claims description 20
- 229920005989 resin Polymers 0.000 claims description 20
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 229940088598 enzyme Drugs 0.000 claims description 9
- 102100024295 Maltase-glucoamylase Human genes 0.000 claims description 4
- 108010028144 alpha-Glucosidases Proteins 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 239000012535 impurity Substances 0.000 abstract description 4
- 150000003384 small molecules Chemical class 0.000 abstract description 4
- 238000000034 method Methods 0.000 description 7
- 235000015165 citric acid Nutrition 0.000 description 6
- 229920002472 Starch Polymers 0.000 description 5
- 235000019698 starch Nutrition 0.000 description 5
- 239000008107 starch Substances 0.000 description 5
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 235000013325 dietary fiber Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 238000009413 insulation Methods 0.000 description 3
- 241000186000 Bifidobacterium Species 0.000 description 2
- 229920000858 Cyclodextrin Polymers 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- DLRVVLDZNNYCBX-RTPHMHGBSA-N isomaltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-RTPHMHGBSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 150000002482 oligosaccharides Chemical class 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- PVXPPJIGRGXGCY-TZLCEDOOSA-N 6-O-alpha-D-glucopyranosyl-D-fructofuranose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)C(O)(CO)O1 PVXPPJIGRGXGCY-TZLCEDOOSA-N 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- ZCLAHGAZPPEVDX-UHFFFAOYSA-N D-panose Natural products OC1C(O)C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC1COC1C(O)C(O)C(O)C(CO)O1 ZCLAHGAZPPEVDX-UHFFFAOYSA-N 0.000 description 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
- 102100022624 Glucoamylase Human genes 0.000 description 1
- AYRXSINWFIIFAE-SCLMCMATSA-N Isomaltose Natural products OC[C@H]1O[C@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)[C@@H](O)[C@@H](O)[C@@H]1O AYRXSINWFIIFAE-SCLMCMATSA-N 0.000 description 1
- 229920001100 Polydextrose Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 235000008452 baby food Nutrition 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 235000021107 fermented food Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- DBTMGCOVALSLOR-AXAHEAMVSA-N galactotriose Natural products OC[C@@H]1O[C@@H](O[C@@H]2[C@@H](O)[C@H](CO)O[C@@H](O[C@H]3[C@@H](O)[C@H](O)O[C@@H](CO)[C@@H]3O)[C@@H]2O)[C@H](O)[C@H](O)[C@H]1O DBTMGCOVALSLOR-AXAHEAMVSA-N 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- FBJQEBRMDXPWNX-FYHZSNTMSA-N isomaltotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC[C@@H]2[C@H]([C@H](O)[C@@H](O)C(O)O2)O)O1 FBJQEBRMDXPWNX-FYHZSNTMSA-N 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- ZCLAHGAZPPEVDX-MQHGYYCBSA-N panose Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@@H](O[C@H]([C@H](O)CO)[C@H](O)[C@@H](O)C=O)O[C@@H]1CO[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 ZCLAHGAZPPEVDX-MQHGYYCBSA-N 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 235000013856 polydextrose Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 235000019991 rice wine Nutrition 0.000 description 1
- 235000021391 short chain fatty acids Nutrition 0.000 description 1
- 150000004666 short chain fatty acids Chemical class 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000003538 tetroses Chemical class 0.000 description 1
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/22—Preparation of compounds containing saccharide radicals produced by the action of a beta-amylase, e.g. maltose
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention discloses a kind of preparation method of the resistant dextrin containing oligoisomaltose, step is as follows：In a kettle., using maltodextrin as raw material, glucose and citric acid are added, solid crude product is made in stirring；Dissolved with 50 90 DEG C of water, obtain lysate；Alpha amylase is added, primary enzymolysis liquid is made；Beta amylase and α glucuroides is added to be saccharified and turned glycosides reaction, obtained secondary enzymolysis liquid；Saccharomyces cerevisiae dry powder is added, then fully fermentation sterilizes and carry out thalline separation, obtain zymotic fluid；Activated carbon decolorizing is carried out, filters afterwards, obtains filtered solution；Ion exchange is carried out, ion exchange liquid is obtained；It is spray-dried, finally gives pale yellow powder finished product.A small amount of oligoisomaltose can also be made in the present invention while obtained resistant dextrin, product yield can be improved, and can effectively be purified, short chain composition, oligomer and the other impurities of the small molecule composition wherein containing glucose are digested and removed.
Description
Technical field
The present invention relates to starch field of deep, the system of specifically a kind of resistant dextrin containing oligoisomaltose
Preparation Method.
Background technology
Resistant dextrin, the important products of starch deep processing are a kind of Low Caloric Polydextroses, belong to low molecular soluble meals
Fiber is eaten, the seventh-largest nutrient necessary to dietary fiber is referred to as health is a kind of important original of modern food industry
Stone.One kind that resistant dextrin belongs in " low viscosity water soluble dietary fiber ", low viscosity is low in calories, low sugariness, high-dissolvability
And good processing stability.Because its difficulty for containing human body digestive ferment (amylase, glucoamylase etc.) effect disappears
Chemical conversion point, will not be digested absorption in alimentary canal, can be directly entered large intestine, promote the Bifidobacterium and lactic acid bacteria of intestinal beneficial
Growth, while suppress harmful intestinal tract microorganism growth and breeding, big enteral ferment produce short chain fatty acids, can play pre-
The effect of anti-colon cancer.In addition, important function of the resistant dextrin also with other physiological functions of regulation human body, such as reduction blood glucose,
Adjust blood fat and control body weight etc..Because resistant dextrin has good physical property and many unique physiological functions, food can be used as
Product and food stone are used, and are a kind of new food additives, be widely used in dairy products, health products, infant food,
In Flour product and meat products.
Oligoisomaltose is also known as isomalto-oligosaccharide, isomaltulose, bifurcation oligose etc., China's light industry mark
Certainly it is oligoisomaltose.It is one kind of starch sugar, and main component is to be combined with α -1,6 glycosidic bonds between glucose molecule
Isomaltose, panose, Isomaltotriose and tetrose more than oligosaccharide.Oligoisomaltose in nature its be used as side chain
The part of starch or polysaccharide.Only exist on a small quantity in some fermented foods such as soy sauce, yellow rice wine.Oligoisomaltose can have
The growth and breeding for promoting beneficial bacteria-Bifidobacterium in human body of effect, therefore also known as " positive growth factor for bifidus ", referred to as
" bifidus factor " can promote the Bifidobacterium in human body significantly to breed due to it, have water-soluble dietary fiber function, and calorific value is low, anti-
The characteristics such as carious tooth, so being kind of a widely used functional oligose.
At present, the preparation method of resistant dextrin is relatively more, and such as high temperature method, microwave method and enzyme process prepare resistant dextrin, greatly
All it is, using starch as base stock, to carry out acidolysis or ferment treatment, product is by resisting that simple purifying subtractive process is obtained more
Property dextrin product.The finished product of obtained resistant dextrin is impure more, during the course of the reaction small molecule monose, oligosaccharide and other
Short chain impurity has no idea to remove, and remains in finished product, causes resistant dextrin purity not high.
The content of the invention
The technical assignment of the present invention is to provide a kind of preparation method of the resistant dextrin containing oligoisomaltose.
The technical assignment of the present invention realizes that the preparation method step is as follows in the following manner：
1）In a kettle., using maltodextrin as raw material, glucose and citric acid is added, is stirred, obtained maltodextrin and mix
Compound, continues to stir, reacts 2-5 hours, solid crude product is made；
2）Gained solid crude product is dissolved with 50-90 DEG C of water, lysate is obtained；
3）It is 5.0-7.0 by lysate regulation pH, temperature is 70-100 DEG C, adds alpha amylase, primary enzymolysis liquid is made；
4）It is 4.0-6.5 by primary enzymolysis liquid regulation pH, when temperature is 55-65 DEG C, adds beta amylase and alpha-glucosaccharase
Enzyme is saccharified and turned glycosides reaction, is fully digested, then the obtained secondary enzymolysis liquid of filtering；
5）It is 5.0-6.5 by secondary enzymolysis liquid regulation pH, adds saccharomyces cerevisiae dry powder, it is permanent under the conditions of temperature is 30-34 DEG C
Warm fully fermentation, then sterilizes and carries out thalline separation, obtain zymotic fluid；
6）Zymotic fluid is carried out to be incubated 20-40min at activated carbon decolorizing, 60-80 DEG C, filters afterwards, obtains filtered solution；
7）Filtered solution is subjected to ion exchange, ion exchange liquid is obtained；
8）Ion exchange liquid is spray-dried, pale yellow powder finished product is finally given.
Described step 1）The weight of middle maltodextrin, glucose and citric acid is as follows：
20-40 parts of maltodextrin, 60-80 parts of glucose, 0.5-3 parts of citric acid.
Described step 1）Middle reaction condition is：Reaction and negative pressure pressure is 0.05-0.1MPa, and reaction temperature is 130-180
℃。
Described step 2）The refractive power of middle lysate is 10-30%.
Described step 3）The addition of middle alpha amylase is every liter of lysate 2.5 × 102-7.5×102U, enzymolysis time is
5-20 hours.
Described step 4）The addition of middle beta amylase is every liter of primary enzymolysis liquid 2.0 × 103-6.0×103U, α-Portugal
The addition of polyglycoside enzyme is every liter of primary enzymolysis liquid 3.0 × 103-9.0×103U, enzymolysis time is 24-60 hours.
Described step 5）The addition of middle saccharomyces cerevisiae dry powder is the 0.8-2.0% of secondary enzymolysis liquid dry matter weight, and
And fermentation time is 20-50 hours.
Described step 6）Middle activated carbon addition is the 1-10% of zymotic fluid dry matter weight.
Described step 7）Middle filtered solution sequentially passes through 001 × 7 positive resin, D301 negative resins, 001 × 7 positive resin, D301
Negative resin carries out ion exchange.
The present invention a kind of resistant dextrin containing oligoisomaltose preparation method compared to the prior art, with
Lower feature：
1）A small amount of oligoisomaltose can also be made while obtained resistant dextrin, it is possible to increase product yield,
And can effectively be purified, short chain composition, oligomer and the other impurities of the small molecule composition wherein containing glucose are obtained
To effectively digesting and remove；
2）In course of reaction, technics comparing is simple, and operation is easy, particularly gentleer to reaction condition, to equipment requirement
It is not high；
3）Resistant dextrin prepared by this method not only ensure that its feature of tool, moreover it is possible to meet the demand of some special populations；
4）This method energy consumption is low, simple to operate, and high income, cost is low.
Embodiment
Embodiment 1：
The preparation method step that this contains the resistant dextrin of oligoisomaltose is as follows：
1）In a kettle., using 35g maltodextrins as raw material, 65g glucose and 0.5g citric acids is added, stirs, obtains
Maltodextrin mixture, continues to stir, reacts 2 hours, and reaction and negative pressure pressure is 0.05MPa, and reaction temperature is 180 DEG C, is made
Solid crude product；
2）Gained solid crude product is dissolved with 50 DEG C of water, the lysate that refractive power is 10% is obtained；
3）It is 6.0 by lysate regulation pH, temperature is 90 DEG C, then every liter of lysate adds alpha amylase 2.5 × 102U, enzymolysis
Time is 10 hours, and primary enzymolysis liquid is made；
4）It is 5.0 by primary enzymolysis liquid regulation pH, when temperature is 55 DEG C, adds beta amylase and alpha-glucosidase carries out sugar
Change and turn glycosides reaction, fully digested, the addition of beta amylase is every liter of primary enzymolysis liquid 2.0 × 103U, phlorose
The addition of glycosides enzyme is every liter of primary enzymolysis liquid 3.0 × 103U, enzymolysis time is 50 hours, then filters and secondary enzymolysis is made
Liquid；
5）It is 5.0 by secondary enzymolysis liquid regulation pH, adds saccharomyces cerevisiae dry powder, the addition of saccharomyces cerevisiae dry powder is secondary enzyme
The 0.8% of liquid dry matter weight is solved, is that under the conditions of 30 DEG C, constant temperature fully ferments 30 hours, then sterilizes and carries out thalline in temperature
Separation, obtains zymotic fluid；
6）Zymotic fluid is subjected to activated carbon decolorizing, activated carbon addition is insulation at the 1% of zymotic fluid dry matter weight, 65 DEG C
40min, is filtered afterwards, obtains filtered solution；
7）Filtered solution is sequentially passed through into 001 × 7 positive resin, D301 negative resins, 001 × 7 positive resin, D301 negative resins and carries out ion
Exchange, obtain ion exchange liquid；
8）Ion exchange liquid is spray-dried, 70g pale yellow powder finished products are finally given, after testing wherein oligomeric different malt
Sugared content is 2.0g.
Embodiment 2：
The preparation method step that this contains the resistant dextrin of oligoisomaltose is as follows：
1）In a kettle., using 25g maltodextrins as raw material, 75g glucose and 1g citric acids is added, stirs, obtains wheat
Bud cyclodextrin mixtures, continue to stir, react 3 hours, and reaction and negative pressure pressure is 0.07MPa, and reaction temperature is 150 DEG C, is made solid
State crude product；
2）Gained solid crude product is dissolved with 70 DEG C of water, the lysate that refractive power is 15% is obtained；
3）It is 6.5 by lysate regulation pH, temperature is 95 DEG C, then every liter of lysate adds alpha amylase 5.0 × 102U, enzymolysis
Time is 10 hours, and primary enzymolysis liquid is made；
4）It is 4.5 by primary enzymolysis liquid regulation pH, when temperature is 60 DEG C, adds beta amylase and alpha-glucosidase carries out sugar
Change and turn glycosides reaction, fully digested, the addition of beta amylase is every liter of primary enzymolysis liquid 3.0 × 103U, phlorose
The addition of glycosides enzyme is every liter of primary enzymolysis liquid 5.0 × 103U, enzymolysis time is 60 hours, then filters and secondary enzymolysis is made
Liquid；
5）It is 6.0 by secondary enzymolysis liquid regulation pH, adds saccharomyces cerevisiae dry powder, the addition of saccharomyces cerevisiae dry powder is secondary enzyme
The 1.2% of liquid dry matter weight is solved, is that under the conditions of 30 DEG C, constant temperature fully ferments 30 hours, then sterilizes and carries out thalline in temperature
Separation, obtains zymotic fluid；
6）Zymotic fluid is subjected to activated carbon decolorizing, activated carbon addition is insulation at the 3% of zymotic fluid dry matter weight, 80 DEG C
25min, is filtered afterwards, obtains filtered solution；
7）Filtered solution is sequentially passed through into 001 × 7 positive resin, D301 negative resins, 001 × 7 positive resin, D301 negative resins and carries out ion
Exchange, obtain ion exchange liquid；
8）Ion exchange liquid is spray-dried, 73g pale yellow powder finished products are finally given, after testing wherein oligomeric different malt
Sugared content is 1.8g.
Embodiment 3：
The preparation method step that this contains the resistant dextrin of oligoisomaltose is as follows：
1）In a kettle., using 30g maltodextrins as raw material, 70g glucose and 3g citric acids is added, stirs, obtains wheat
Bud cyclodextrin mixtures, continue to stir, react 5 hours, and reaction and negative pressure pressure is 0.1MPa, and reaction temperature is 130 DEG C, and solid-state is made
Crude product；
2）Gained solid crude product is dissolved with 80 DEG C of water, the lysate that refractive power is 20% is obtained；
3）It is 6.0 by lysate regulation pH, temperature is 90 DEG C, then every liter of lysate adds alpha amylase 3.5 × 102U, enzymolysis
Time is 10 hours, and primary enzymolysis liquid is made；
4）It is 5.0 by primary enzymolysis liquid regulation pH, when temperature is 55 DEG C, adds beta amylase and alpha-glucosidase carries out sugar
Change and turn glycosides reaction, fully digested, the addition of beta amylase is every liter of primary enzymolysis liquid 4.0 × 103U, phlorose
The addition of glycosides enzyme is every liter of primary enzymolysis liquid 5.0 × 103U, enzymolysis time is 40 hours, then filters and secondary enzymolysis is made
Liquid；
5）It is 6.5 by secondary enzymolysis liquid regulation pH, adds saccharomyces cerevisiae dry powder, the addition of saccharomyces cerevisiae dry powder is secondary enzymolysis
The 1.8% of liquid dry matter weight, under the conditions of temperature is 32 DEG C, constant temperature fully ferments 32 hours, then sterilizes and carries out thalline and divides
From acquisition zymotic fluid；
6）Zymotic fluid is subjected to activated carbon decolorizing, activated carbon addition is insulation at the 5% of zymotic fluid dry matter weight, 70 DEG C
30min, is filtered afterwards, obtains filtered solution；
7）Filtered solution is sequentially passed through into 001 × 7 positive resin, D301 negative resins, 001 × 7 positive resin, D301 negative resins and carries out ion
Exchange, obtain ion exchange liquid；
8）Ion exchange liquid is spray-dried, 75g pale yellow powder finished products are finally given, after testing wherein oligomeric different malt
Sugared content is 2.2g.
By the above method, a small amount of oligoisomaltose, energy can also be made while obtained resistant dextrin
Product yield is enough improved, and can effectively be purified, by the short chain composition of the small molecule composition wherein containing glucose, oligomeric
Thing and other impurities are effectively digested and removed.
By embodiment above, the those skilled in the art can readily realize the present invention.But should
Work as understanding, the present invention is not limited to above-mentioned several embodiments.On the basis of disclosed embodiment, the technology
The technical staff in field can be combined different technical characteristics, so as to realize different technical schemes.
Claims (9)
1. a kind of preparation method of the resistant dextrin containing oligoisomaltose, it is characterised in that the preparation method step is as follows：
1）In a kettle., using maltodextrin as raw material, glucose and citric acid is added, is stirred, obtained maltodextrin and mix
Compound, continues to stir, reacts 2-5 hours, solid crude product is made；
2）Gained solid crude product is dissolved with 50-90 DEG C of water, lysate is obtained；
3）It is 5.0-7.0 by lysate regulation pH, temperature is 70-100 DEG C, adds alpha amylase, primary enzymolysis liquid is made；
4）It is 4.0-6.5 by primary enzymolysis liquid regulation pH, when temperature is 55-65 DEG C, adds beta amylase and alpha-glucosaccharase
Enzyme is saccharified and turned glycosides reaction, is fully digested, then the obtained secondary enzymolysis liquid of filtering；
5）It is 5.0-6.5 by secondary enzymolysis liquid regulation pH, adds saccharomyces cerevisiae dry powder, it is permanent under the conditions of temperature is 30-34 DEG C
Warm fully fermentation, then sterilizes and carries out thalline separation, obtain zymotic fluid；
6）Zymotic fluid is carried out to be incubated 20-40min at activated carbon decolorizing, 60-80 DEG C, filters afterwards, obtains filtered solution；
7）Filtered solution is subjected to ion exchange, ion exchange liquid is obtained；
8）Ion exchange liquid is spray-dried, pale yellow powder finished product is finally given.
2. a kind of preparation method of resistant dextrin containing oligoisomaltose according to claim 1, it is characterised in that
Described step 1）The weight of middle maltodextrin, glucose and citric acid is as follows：
20-40 parts of maltodextrin, 60-80 parts of glucose, 0.5-3 parts of citric acid.
3. a kind of preparation method of resistant dextrin containing oligoisomaltose according to claim 1 or 2, its feature exists
In described step 1）Middle reaction condition is：Reaction and negative pressure pressure is 0.05-0.1MPa, and reaction temperature is 130-180 DEG C.
4. a kind of preparation method of resistant dextrin containing oligoisomaltose according to claim 1, it is characterised in that
Described step 2）The refractive power of middle lysate is 10-30%.
5. a kind of preparation method of resistant dextrin containing oligoisomaltose according to claim 1, it is characterised in that
Described step 3）The addition of middle alpha amylase is every liter of lysate 2.5 × 102-7.5×102U, enzymolysis time is that 5-20 is small
When.
6. a kind of preparation method of resistant dextrin containing oligoisomaltose according to claim 1, it is characterised in that
Described step 4）The addition of middle beta amylase is every liter of primary enzymolysis liquid 2.0 × 103-6.0×103U, alpha-glucosidase
Addition be every liter of primary enzymolysis liquid 3.0 × 103-9.0×103U, enzymolysis time is 24-60 hours.
7. a kind of preparation method of resistant dextrin containing oligoisomaltose according to claim 1, it is characterised in that
Described step 5）The addition of middle saccharomyces cerevisiae dry powder is the 0.8-2.0% of secondary enzymolysis liquid dry matter weight, and when fermenting
Between be 20-50 hours.
8. a kind of preparation method of resistant dextrin containing oligoisomaltose according to claim 1, it is characterised in that
Described step 6）Middle activated carbon addition is the 1-10% of zymotic fluid dry matter weight.
9. a kind of preparation method of resistant dextrin containing oligoisomaltose according to claim 1, it is characterised in that
Described step 7）Middle filtered solution sequentially passes through 001 × 7 positive resin, D301 negative resins, 001 × 7 positive resin, D301 negative resins and entered
Row ion exchange.
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| CN109275770A (en) * | 2018-06-25 | 2019-01-29 | 聂菲菲 | A kind of konjak oligosaccharide buccal tablets |
| CN113430238A (en) * | 2021-06-08 | 2021-09-24 | 江南大学 | Method for producing resistant dextrin by adding sucrose/fructo-oligosaccharide |
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| CN106046182A (en) * | 2016-08-24 | 2016-10-26 | 山东福田药业有限公司 | Resistant dextrin synthesis technology |
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| CN101870991A (en) * | 2010-06-08 | 2010-10-27 | 保龄宝生物股份有限公司 | Method for preparing novel anti-peptic dextrin |
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| CN113430238A (en) * | 2021-06-08 | 2021-09-24 | 江南大学 | Method for producing resistant dextrin by adding sucrose/fructo-oligosaccharide |
| CN113430238B (en) * | 2021-06-08 | 2022-10-11 | 江南大学 | Method for producing resistant dextrin by adding sucrose/fructo-oligosaccharide |
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