CN108118399A - A kind of preparation method of collagen film - Google Patents
A kind of preparation method of collagen film Download PDFInfo
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- CN108118399A CN108118399A CN201711092035.2A CN201711092035A CN108118399A CN 108118399 A CN108118399 A CN 108118399A CN 201711092035 A CN201711092035 A CN 201711092035A CN 108118399 A CN108118399 A CN 108118399A
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- preparation
- collagen film
- fish
- emulsion
- protein liquid
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/732—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by fluid current, e.g. air-lay
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- Engineering & Computer Science (AREA)
- Textile Engineering (AREA)
- Mechanical Engineering (AREA)
- Materials For Medical Uses (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- General Preparation And Processing Of Foods (AREA)
Abstract
The invention discloses a kind of preparation method of collagen film, method includes pretreatment, the preparation of protein liquid and the preparation of film of Optimization of Low Value Fish.It has the beneficial effect that：Process is easy to operate, reproducible, and applied widely, can be amplified according to actual conditions, realizes industrialized production；3 added in, sucrose fatty ester in 5 resacetophenones, aziridine and emulsion has synergistic effect, the mechanical property and bioactivity of collagen film are improved, reduces its hemolytic and antigenicity, drastically increases the economic value of collagen film.
Description
Technical field
The present invention relates to technical field of biological product preparation, more particularly, to a kind of preparation method of collagen film.
Background technology
Due to the popularization of modern Fishing technology, larger fish resource is gradually destroyed, so that small fish in biological chain,
Shrimp breeds rapidly.In addition, the small fish, shrimp and disposal from fishery product processing in catches typically constitute from the 28% of catches.By
In the further investigation for lacking process and utilization technology, low value fish are mainly for the production of the low value-added product such as fish meal at present, even
It is directly abandoned as discarded object, not only waste of resource, but also also results in the pollution of ocean, terrestrial environment.It if can be by Optimization of Low Value Fish
It further develops and utilizes, the added value of Optimization of Low Value Fish and its by-product will be greatly improved, for this marine resources is made full use of to open
Ward off new approach.
The content of the invention
It is an object of the invention to provide a kind of preparation methods of collagen film, and this method is easy to operate, reproducible,
The mechanical property and bioactivity of the collagen film of preparation are excellent, and hemolytic and antigenicity are low.
The problem of present invention in background technology for mentioning, the technical solution taken is：A kind of preparation of collagen film
Method, method comprise the following steps：
The pretreatment of Optimization of Low Value Fish：Optimization of Low Value Fish fish scale, fish-bone and fish-skin leftover bits and pieces are taken, in the food that mass fraction is 22%-25%
Boiling in brine, boiling temperature are 81-87 DEG C, and time 17-21min is cooled to room temperature, wherein, leftover bits and pieces and saline solution
Mass ratio is 1/1.2-1.5, and preprocessing process avoids the influence of impurity, while brine digestion process plays the effect of sterilization
Fruit, and the protein in leftover bits and pieces can be moderately denatured at this temperature, and good condition is provided for the preparation of following protein liquid；
The preparation of protein liquid：Leftover bits and pieces is put into pressure cooker, the boiling at absolute pressure 0.15-0.2MPa, 121-126 DEG C of temperature
0.6-1h obtains protein liquid, and under the conditions of being somebody's turn to do, the albumen dissolution rate contained by leftover bits and pieces is fast, and dissolution rate is high, obtains low value fish resource
It makes full use of, substantially increases the economic value of method；
The preparation of emulsion：Stearic acid and sucrose fatty ester are added in into aqua sterilisa, by gained mixed liquor in 75-85 DEG C of heating
Dissolving, then carries out homogenization with tissue mashing machine, obtains emulsion.Wherein, homogeneous rotating speed be 20000-25000rpm, homogeneous
Time is 2-3min, and stearic mass fraction is 0.3%-1.8% in mixed liquor, and the mass fraction of sucrose fatty ester is
0.1%-3.2%；
The preparation of film：Protein liquid and emulsion for 2-2.5/1 are mixed by volume, adds and accounts for protein liquid quality 0.02%-
0.03% 3,5- resacetophenones and the aziridine of 0.03%-0.05%, then carry out electrostatic spinning, and fltting speed is
0.6-1mL/h receives distance as 13-14cm, and voltage is 16-18kV to get collagen film.The process is easy to operate, repeats
Property it is good and applied widely, can be amplified according to actual conditions, realize industrialized production；The 3,5- dihydroxy benzenes added in
Sucrose fatty ester in ethyl ketone, aziridine and emulsion has synergistic effect, improves the mechanical property of collagen film
And bioactivity, its hemolytic and antigenicity are reduced, drastically increases the economic value of collagen film.
Compared with prior art, the advantage of the invention is that：A kind of preparation method of collagen film disclosed by the invention
The preparation of pretreatment, protein liquid and the preparation of film including Optimization of Low Value Fish, process is easy to operate, reproducible, and the scope of application
Extensively, it can be amplified according to actual conditions, realize industrialized production；The 3,5- resacetophenones that are added in, aziridine with
Sucrose fatty ester in emulsion has synergistic effect, improves the mechanical property and bioactivity of collagen film, reduces
Its hemolytic and antigenicity drastically increase the economic value of collagen film.
Specific embodiment
Below by embodiment, the present invention will be further described：
Embodiment 1：
A kind of preparation method of collagen film, the described method comprises the following steps：
1) pretreatment of Optimization of Low Value Fish：Optimization of Low Value Fish fish scale, fish-bone and fish-skin leftover bits and pieces are taken, in the saline solution that mass fraction is 22%
Middle boiling, boiling temperature are 81 DEG C, and time 17min is cooled to room temperature, wherein, the mass ratio of leftover bits and pieces and saline solution is 1/
1.2, preprocessing process avoids the influence of impurity, while brine digestion process plays the effect of sterilization, and at this temperature under
Protein in heel can be moderately denatured, and good condition is provided for the preparation of following protein liquid；
2) preparation of protein liquid：Leftover bits and pieces is put into pressure cooker, the boiling 0.6h at absolute pressure 0.15MPa, 121 DEG C of temperature,
Protein liquid is obtained, under the conditions of being somebody's turn to do, the albumen dissolution rate contained by leftover bits and pieces is fast, and dissolution rate is high, and low value fish resource is made to have obtained fully
It utilizes, substantially increases the economic value of method；
3) preparation of emulsion：Stearic acid and sucrose fatty ester are added in into aqua sterilisa, by gained mixed liquor in 75 DEG C of heating
Dissolving, then carries out homogenization with tissue mashing machine, obtains emulsion.Wherein, homogeneous rotating speed is 20000rpm, and homogenizing time is
2min, stearic mass fraction is 0.3% in mixed liquor, and the mass fraction of sucrose fatty ester is 0.1%；
4) preparation of film：It is by volume 2/1 mixing by protein liquid and emulsion, adds and account for the 3 of protein liquid quality 0.02%,
5- resacetophenones and 0.03% aziridine, then carry out electrostatic spinning, and fltting speed 0.6mL/h receives distance
For 13cm, voltage is 16kV to get collagen film.The process is easy to operate, reproducible, and applied widely, can basis
Actual conditions are amplified, and realize industrialized production；In the 3,5- resacetophenones, aziridine and the emulsion that are added in
Sucrose fatty ester has synergistic effect, improves the mechanical property and bioactivity of collagen film, reduces its hemolytic
And antigenicity, drastically increase the economic value of collagen film.
Embodiment 2：
A kind of preparation method of collagen film, the described method comprises the following steps：It is the pretreatment of Optimization of Low Value Fish first：Take low value
Fish fish scale, fish-bone and fish-skin leftover bits and pieces, the boiling in mass fraction is 25% saline solution, boiling temperature are 87 DEG C, the time
For 21min, it is cooled to room temperature, wherein, the mass ratio of leftover bits and pieces and saline solution is 1/1.5；Followed by the preparation of protein liquid：By under
Heel is put into pressure cooker, and the boiling 1h at absolute pressure 0.2MPa, 126 DEG C of temperature obtains protein liquid；Followed by the system of emulsion
It is standby：Stearic acid and sucrose fatty ester are added in into aqua sterilisa, gained mixed liquor at 85 DEG C is dissolved by heating, is then smash with tissue
Broken machine carries out homogenization, obtains emulsion.Wherein, homogeneous rotating speed is 25000rpm, homogenizing time 3min, stearic in mixed liquor
The mass fraction of acid is 1.8%, and the mass fraction of sucrose fatty ester is 3.2%；It is finally the preparation of film：By protein liquid and breast
It is by volume 2.5/1 mixing to change liquid, adds 3, the 5- resacetophenones that account for protein liquid quality 0.03% and 0.05%
Aziridine then carries out electrostatic spinning, and fltting speed 1mL/h receives distance as 14cm, and voltage is 18kV to get collagen
Protein film.The process is easy to operate, reproducible, and applied widely, can be amplified according to actual conditions, realizes industrialization
Production；Sucrose fatty ester in 3, the 5- resacetophenones, aziridine and the emulsion that are added in has synergistic effect, carries
The high mechanical property and bioactivity of collagen film, reduces its hemolytic and antigenicity, drastically increases collagen egg
The economic value of tunica albuginea.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of preparation method of collagen film, it is characterised in that：It the described method comprises the following steps：
1) pretreatment of Optimization of Low Value Fish：Optimization of Low Value Fish fish scale, fish-bone and fish-skin leftover bits and pieces is taken to be steamed in the saline solution that temperature is 81-87 DEG C
17-21min is boiled, is cooled to room temperature；
2) preparation of protein liquid：Leftover bits and pieces is put into pressure cooker, is steamed at absolute pressure 0.15-0.2MPa, 121-126 DEG C of temperature
0.6-1h is boiled, obtains protein liquid；
3) preparation of emulsion：Stearic acid and sucrose fatty ester are added in into aqua sterilisa, gained mixed liquor is added at 75-85 DEG C
Heat of solution then carries out homogenization with tissue mashing machine, obtains emulsion；
4) preparation of film：Protein liquid and emulsion are mixed, add 3, the 5- dihydroxies for accounting for protein liquid quality 0.02%-0.03%
The aziridine of benzoylformaldoxime and 0.03%-0.05% then carries out electrostatic spinning to get collagen film.
2. a kind of preparation method of collagen film according to claim 1, it is characterised in that：Salt in the step 1)
Mass fraction for 22%-25%, the mass ratio of leftover bits and pieces and saline solution is 1/1.2-1.5.
3. a kind of preparation method of collagen film according to claim 1, it is characterised in that：Homogeneous in the step 3)
Rotating speed is 20000-25000rpm, homogenizing time 2-3min.
4. a kind of preparation method of collagen film according to claim 1, it is characterised in that：Step 3) the mixed liquor
In stearic mass fraction be 0.3%-1.8%, the mass fraction of sucrose fatty ester is 0.1%-3.2%.
5. a kind of preparation method of collagen film according to claim 1, it is characterised in that：Albumen in the step 4)
Liquid and emulsion are 2-2.5/1 by volume.
6. a kind of preparation method of collagen film according to claim 1, it is characterised in that：Electrostatic in the step 4)
The condition of spinning is：Fltting speed is 0.6-1mL/h, and it is 13-14cm, voltage 16-18kV to receive distance.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711092035.2A CN108118399A (en) | 2017-11-08 | 2017-11-08 | A kind of preparation method of collagen film |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711092035.2A CN108118399A (en) | 2017-11-08 | 2017-11-08 | A kind of preparation method of collagen film |
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| CN108118399A true CN108118399A (en) | 2018-06-05 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101942204A (en) * | 2010-09-16 | 2011-01-12 | 集美大学 | Production method of collagen membrane |
| CN102504302A (en) * | 2011-11-08 | 2012-06-20 | 集美大学 | Method for preparing collagen film |
| CN103046225A (en) * | 2012-01-19 | 2013-04-17 | 苏州达普生物技术有限公司 | Preparation method of collagen membrane |
| CN103540635A (en) * | 2013-09-23 | 2014-01-29 | 石狮海星食品有限公司 | Preparation process of fish scale collagen protein |
| CN104082520A (en) * | 2014-07-16 | 2014-10-08 | 常熟理工学院 | Low-value marine fish peptide meal as well as preparation method and application thereof |
| CN106421903A (en) * | 2016-08-17 | 2017-02-22 | 东华大学 | Blue shark fishskin collagen periodontium guided regeneration nanofiber membrane and preparing method thereof |
-
2017
- 2017-11-08 CN CN201711092035.2A patent/CN108118399A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101942204A (en) * | 2010-09-16 | 2011-01-12 | 集美大学 | Production method of collagen membrane |
| CN102504302A (en) * | 2011-11-08 | 2012-06-20 | 集美大学 | Method for preparing collagen film |
| CN103046225A (en) * | 2012-01-19 | 2013-04-17 | 苏州达普生物技术有限公司 | Preparation method of collagen membrane |
| CN103540635A (en) * | 2013-09-23 | 2014-01-29 | 石狮海星食品有限公司 | Preparation process of fish scale collagen protein |
| CN104082520A (en) * | 2014-07-16 | 2014-10-08 | 常熟理工学院 | Low-value marine fish peptide meal as well as preparation method and application thereof |
| CN106421903A (en) * | 2016-08-17 | 2017-02-22 | 东华大学 | Blue shark fishskin collagen periodontium guided regeneration nanofiber membrane and preparing method thereof |
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Application publication date: 20180605 |
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