CN111603434B

CN111603434B - Composition for improving skin wrinkles and cosmetic thereof

Info

Publication number: CN111603434B
Application number: CN202010494137.2A
Authority: CN
Inventors: 王猛; 苏丹辉; 刘瑞学; 张颐和
Original assignee: Henan Shangyan Biotechnology Co ltd; Guangzhou Zhishang Biology Biology Co ltd
Current assignee: Henan Shangyan Biotechnology Co ltd; Guangzhou Zhishang Biology Biology Co ltd
Priority date: 2020-06-03
Filing date: 2020-06-03
Publication date: 2023-06-06
Anticipated expiration: 2040-06-03
Also published as: CN111603434A

Abstract

The invention discloses a composition for improving skin wrinkles and a cosmetic thereof, and aims to provide a composition which has the advantages of synergistic effect of all components, effective free radical removal, strong oxidation resistance, collagen synthesis promotion and skin aging delay, and mainly comprises the following components in parts by weight: 5-10 parts of ginseng root extract, 2-7 parts of rosewood heart wood fruit extract, 2-6 parts of sphingomonas fermentation product extract and 0.5-3 parts of sleeper leaf extract; belongs to the technical field of cosmetics.

Description

Composition for improving skin wrinkles and cosmetic thereof

Technical Field

The invention discloses a composition, in particular relates to a composition for improving skin wrinkles, and belongs to the technical field of cosmetics.

Background

Skin aging is a physiological process acted by various factors, and the skin cannot adapt to the change of internal and external environments due to the weakening of the protective capability and the regulating capability of the skin to the organism, so that the overall appearance conditions of the skin, such as the state, the color, the texture and the like, are changed. And along with the increasingly prominent problems of environmental pollution, social pressure and the like, the aging phenomena of wrinkles, slackening, dullness and the like of the skin become more serious.

Aging of skin is classified into endogenous aging and exogenous aging. Endogenous aging refers to natural aging of skin with aging, which is manifested by skin whitening, appearance of fine wrinkles, reduced elasticity, and loose skin. The most important causes of extrinsic aging are photoaging due to sun exposure, manifested by wrinkles, skin sagging, rough, yellowish or yellowish skin discoloration, telangiectasia, pigmentation, etc. The most important factor causing endogenous aging is free radical, which is a very active group of active molecules, usually the product of metabolism of human body, and to prevent free radical damage, the organism has endogenous antioxidants such as glutathione, coenzyme Q10 and SOD to prevent invasion of free radicals. However, the damage to the body caused by the environmental factors causing exogenous aging can also increase free radicals, such as pollution, ultraviolet rays, sunlight and the like, can destroy the antioxidant system of the skin, reduce the activity level of antioxidant enzymes and cause accumulation of lipid peroxides, and the non-enzyme free radical scavenger is exhausted, so that the skin is damaged, and the damaged antioxidant defense system of the skin can generate more free radicals. The increase of free radicals can prevent the synthesis of collagen and elastin and oxidize healthy cells, so that the skin aging of a human body is accelerated and wrinkles are increased. Therefore, the improvement of wrinkles and the anti-aging are required to be free radical resistant and oxidation resistant to a great extent.

At present, products for delaying skin aging through antioxidant effect on the market are various, but some antioxidant skin care products mainly achieve the effect of removing free radicals through chemical reagents, skin damage can be caused after long-term use, health hidden danger is caused, or the antioxidant skin care products have limited capability of removing free radicals and poor effect of improving wrinkles. Therefore, there is a need to develop a composition which is safe, non-irritating and has an ideal antioxidant effect and which can be applied in products for improving skin wrinkles and anti-aging.

Disclosure of Invention

Aiming at the defects, the invention aims to provide a composition for improving skin wrinkles, which has the advantages that all the components are synergistic, free radicals can be effectively removed, and the oxidation resistance is high, so that the synthesis of collagen is promoted, and the composition can be applied to cosmetics to achieve the effects of reducing skin wrinkles and delaying skin aging.

For this purpose, the first technical solution provided by the present invention is as follows:

a composition for improving skin wrinkles mainly comprises the following components in parts by weight: 5-10 parts of ginseng root extract, 2-7 parts of rosewood heart wood fruit extract, 2-6 parts of sphingomonas fermentation product extract and 0.5-3 parts of sleeping dish leaf extract.

The ginseng root extract is rich in various active ingredients such as ginsenoside, amino acid, saccharide, vitamin and the like, has the effects of scavenging free radicals, resisting oxidation and inhibiting elastase activity, can improve the content of hydroxyproline in skin, ensures that the skin is smooth and fine, and achieves the effects of delaying skin aging and increasing skin elasticity.

The myrtle fruit essence is rich in natural vitamin C with extremely high concentration which is 30 times that of citrus, and the extract has powerful antioxidant activity, and can eliminate and resist free radical, promote collagen synthesis, prevent collagen fiber degradation, limit the release of proinflammatory cytokines, maintain the tissue architecture of skin and restore the healthy and young state of skin.

The sphingomonas fermentation product extract is obtained by fermentation of the sphingomonas, can resist active oxygen free radicals, increase the antioxidant capacity, inhibit the expression of cell senescence-associated proteins p21Waf1 and p16INK4a, rebuild extracellular matrix, restore the structural integrity of dermis, increase the expression of collagen and fibrin, improve the compactness and elasticity of skin and prevent the aging of skin cells.

The sleeping vegetable leaf extract has strong antioxidant capacity, can promote synthesis of more collagen and elastin, tighten skin, promote facial contour, stabilize and protect vitamin C, increase synthesis of collagen, and achieve the effect of increasing skin smoothness.

Further, the composition for improving skin wrinkles provided by the invention further comprises 2-4 parts of the herba schizophragmatis integrifolii radicis extract. The extract of the herba geenae Geranii, also called white snow tea, can remove free radicals, exert antioxidant activity, obviously promote proliferation of skin fibroblasts, effectively improve synthesis of type I collagen, improve expression of hyaluronic acid by the fibroblasts, and have good moisturizing and anti-aging effects.

Further, the composition for improving skin wrinkles provided by the invention further comprises 1-4 parts of hydrolyzed yeast protein. The hydrolyzed yeast protein contains amino acid, small peptide, abundant B vitamins, glutathione and nucleotide substances, can resist free radicals and promote the synthesis of collagen.

Further, the ginseng root in the composition for improving skin wrinkles provided by the invention is black ginseng root. The black ginseng is obtained by steaming white ginseng, repeatedly steaming and sun drying, contains more active ingredients such as ginsenoside, and has stronger biological activity.

Further, the weight ratio of the ginseng root extract to the myrtle extract in the composition for improving skin wrinkles is 3:2.

Further, the preparation method of the extract of the myrtus tomentosa in the composition for improving skin wrinkles provided by the invention comprises the following steps:

(1) Pretreatment of raw materials: cleaning the fruit of the pseudo-myrtle, integrally crushing, putting into a colloid mill, and circularly pulping to prepare pulp of the pseudo-myrtle;

(2) Enzymolysis: adding 10 times of water into the pulp of the pseudo-peach fruit, adding cellulose with the weight of 0.3% and the enzyme activity of 1000U/g into the pulp of the pseudo-peach fruit, carrying out enzymolysis for 1-3 hours at the constant temperature of 40-55 ℃ under the condition that the pH=4.0-5.5, and finally heating the enzymolysis liquid to 100 ℃ to inactivate for 10 minutes, and cooling to room temperature for standby;

(3) Microwave treatment: performing microwave treatment on the enzymolysis liquid in the step (2), wherein the microwave treatment conditions are as follows: the power is 200-400W, the temperature is 30-50 ℃ and the time is 2-5min;

(4) Fermentation: inoculating 0.5-1.5% of Kluyveromyces lactis culture medium into the mixed solution in the step (3), uniformly mixing, placing the mixture in a fermentation tank, fermenting and culturing for 3-5 days on a shaking table with the rotating speed of 100-150r/min at the constant temperature of 30-35 ℃;

(5) Purifying: sterilizing the fermentation liquor in the step (4) at a high temperature of 121 ℃ for 30min, cooling, centrifuging to obtain a supernatant, loading the supernatant onto an H-60 macroporous adsorption resin column, wherein the loading amount is 3-5 times of the volume of the column, the loading flow rate is 1.0mL/min, standing for 1H after loading is finished, eluting with 4 times of the volume of the column, discarding the eluent, eluting with 5-6 times of 50-60% ethanol solution, recovering the eluent, and concentrating under reduced pressure to dryness to obtain the pseudo-myrtle fruit extract.

Further, the concentration of the kluyveromyces lactis in the step (4) in the preparation method of the pseudo-myrtle fruit extract is as follows: 10 ⁷ -10 ⁸ CFU/mL, the culture medium in the step (4) comprises the following components: 10.0g of peptone, 8.0g of beef powder, 5.0g of yeast powder, 20.0g of glucose, 801.0mL of tween, 2.0g of dipotassium hydrogen phosphate, 5.0g of sodium acetate, 2.0g of diammonium hydrogen citrate, 0.5g of magnesium sulfate and 0.05g of manganese sulfate, and the volume is fixed to 1000mL by deionized water.

In addition, in order to make the stability of the composition of the invention better, 0.1-1 part of preservative can be added into the composition, and the preservative can be one or a combination of a plurality of conventional preservatives such as phenoxyethanol, sodium benzoate and the like.

The second technical scheme provided by the invention is as follows:

a cosmetic comprises the composition for improving skin wrinkles and common matrix and/or deionized water for external preparation.

Specifically, the conventional base for the external cosmetic dosage form contains one or more of an emulsifier, a fat, an emollient, a humectant, a thickener, a pH adjustor, a polyhydric alcohol, a preservative, a coloring matter and an essence.

The type of the above cosmetic preparation is not particularly limited, and is preferably water, cream, emulsion, essence, gel, or jelly.

Compared with the prior art, the invention has the following beneficial effects:

(1) The composition has strong oxidation resistance through reasonable collocation of the ginseng root extract, the myrtle fruit extract, the sphingomonas fermentation product extract and the sleeping dish leaf extract, can effectively resist the invasion of free radicals to organisms, prevent collagen from degrading, promote the synthesis of collagen, further improve and reduce wrinkles, strengthen skin elasticity and compactness and prevent skin aging. When the ginseng root is black ginseng root, the capacity of scavenging free radicals is stronger, and the ginseng root extract and the rosewood heart wood fruit extract can play a better role in resisting oxidation between the compositions under a specific proportion, so that the effects of improving wrinkles and resisting aging are more obvious. The composition can be added with tea extract and hydrolyzed yeast protein, and has effects of further enhancing free radical scavenging ability, removing wrinkles, and relieving aging signs.

(2) The extract of the pseudo-myrtle extracts is prepared by biological enzymolysis and microwave-assisted fermentation technology, has mild reaction conditions and high reaction efficiency, and can better play an antioxidant effect by compounding the obtained extract with other components under proper enzymatic hydrolysis and microbial fermentation conditions, reduce the generation of facial wrinkles, enable the skin to be tender and compact and restore youth.

Detailed Description

The following claims are presented in further detail in connection with the detailed description, but are not to be construed as limiting the invention, as any person who makes a limited number of modifications within the scope of the claims is within the scope of the claims.

Example 1

The invention provides a composition for improving skin wrinkles, which comprises the following components in parts by weight: 5g of ginseng root extract, 7g of rosewood fruit extract, 6g of Sphingomonas fermentation product extract and 2g of sleeping vegetable leaf extract.

Example 2

The invention provides a composition for improving skin wrinkles, which comprises the following components in parts by weight: 8g of ginseng root extract, 5g of rosewood fruit extract, 4g of Sphingomonas fermentation product extract and 3g of sleeping vegetable leaf extract.

The extract of the Pelargonium gracilis in examples 1-2 is prepared by the following steps in order:

(2) Enzymolysis: adding 10 times of water into the pulp of the pseudo-peach fruits, adding cellulose with the weight of 0.3% and the enzyme activity of 1000U/g into the pulp of the pseudo-peach fruits, carrying out enzymolysis for 3 hours at the constant temperature of 40 ℃ at the pH of 4.5, heating the enzymolysis liquid to 100 ℃ to inactivate for 10 minutes, and cooling to room temperature for standby;

(3) Microwave treatment: performing microwave treatment on the enzymolysis liquid in the step (2), wherein the microwave treatment conditions are as follows: the power is 250W, the temperature is 40 ℃ and the time is 3min;

(4) Fermentation: inoculating 0.5% of Kluyveromyces lactis culture medium into the mixed solution in the step (3), uniformly mixing, placing the mixture in a fermentation tank, and fermenting and culturing on a shaking table with the rotating speed of 100r/min for 4 days at the constant temperature of 35 ℃;

(5) Purifying: sterilizing the fermentation liquor in the step (4) at the high temperature of 121 ℃ for 30min, cooling, centrifuging to obtain a supernatant, loading the supernatant onto an H-60 macroporous adsorption resin column, wherein the loading amount is 3 times of the volume of the column, the loading flow rate is 1.0mL/min, standing for 1H after loading is finished, eluting with 4 times of the volume of the column, discarding the eluent, eluting with 5 times of the volume of an ethanol solution with the concentration of 60%, recovering the eluent, and concentrating under reduced pressure to dryness to obtain the extract of the myrtle fruit.

The concentration of the kluyveromyces lactis in the step (4) is as follows: 10 ⁷ CFU/mL, the culture medium in the step (4) comprises the following components: 10.0g of peptone, 8.0g of beef powder, 5.0g of yeast powder, 20.0g of glucose, 801.0mL of tween, 2.0g of dipotassium hydrogen phosphate, 5.0g of sodium acetate, 2.0g of diammonium hydrogen citrate, 0.5g of magnesium sulfate and 0.05g of manganese sulfate, and the volume is fixed to 1000mL by deionized water.

The ginseng root extract described in examples 1-2 is an extract of white ginseng root.

Example 3

The extract of the Pelargonium gracilis in example 3 is prepared by the following steps in sequence:

Example 4

The invention provides a composition for improving skin wrinkles, which comprises the following components in parts by weight: 10g of ginseng root extract, 4.5g of rosewood fruit extract, 3g of Sphingomonas fermentation product extract, 0.5g of sleeping bag leaf extract and 2g of ground tea leaf extract.

Example 5

The invention provides a composition for improving skin wrinkles, which comprises the following components in parts by weight: 6g of ginseng root extract, 7g of rosewood fruit extract, 2g of sphingomonas fermentation product extract, 1g of sleeping vegetable leaf extract and 4g of ground tea leaf extract.

The extract of the Pelargonium gracilis in examples 4-5 was prepared by the following steps in order:

(2) Enzymolysis: adding 10 times of water into the pulp of the pseudo-peach fruit, adding cellulose with the weight of 0.3% and the enzyme activity of 1000U/g into the pulp of the pseudo-peach fruit, carrying out enzymolysis for 1h at the constant temperature of 55 ℃ at the pH of=4.0, heating the enzymolysis liquid to 100 ℃ to inactivate for 10min, and cooling to room temperature for standby;

(3) Microwave treatment: performing microwave treatment on the enzymolysis liquid in the step (2), wherein the microwave treatment conditions are as follows: the power is 200W, the temperature is 50 ℃ and the time is 2min;

(4) Fermentation: inoculating 1.0% of Kluyveromyces lactis culture medium into the mixed solution in the step (3), uniformly mixing, placing the mixture in a fermentation tank, and fermenting and culturing on a shaking table with the rotating speed of 150r/min for 3 days at the constant temperature of 30 ℃;

(5) Purifying: sterilizing the fermentation liquor in the step (4) at the high temperature of 121 ℃ for 30min, cooling, centrifuging to obtain a supernatant, loading the supernatant onto an H-60 macroporous adsorption resin column, wherein the loading amount is 5 times of the volume of the column, the loading flow rate is 1.0mL/min, standing for 1H after loading is finished, eluting with 4 times of the volume of the column, discarding the eluent, eluting with 6 times of the volume of 50% ethanol solution, the eluting rate is 2.0BV/H, recovering the eluent, and concentrating under reduced pressure to dryness to obtain the extract of the myrtle fruit.

The concentration of the kluyveromyces lactis in the step (4) is as follows: 10 ⁸ CFU/mL, the culture medium in the step (4) comprises the following components: peptone 10.0g, beef powder8.0g of yeast powder, 5.0g of glucose, 20.0g of tween 801.0mL, 2.0g of dipotassium hydrogen phosphate, 5.0g of sodium acetate, 2.0g of diammonium hydrogen citrate, 0.5g of magnesium sulfate and 0.05g of manganese sulfate, and the volume is fixed to 1000mL by deionized water.

Example 6

The invention provides a composition for improving skin wrinkles, which comprises the following components in parts by weight: 6g of ginseng root extract, 2g of rosewood fruit extract, 3g of sphingomonas fermentation product extract, 2g of sleeping vegetable leaf extract, 3g of ground tea leaf extract, 4g of hydrolyzed yeast protein, 0.4g of phenoxyethanol and 0.1g of sodium benzoate.

The extract of the Pelargonium gracilis in example 6 is prepared by the following steps in sequence:

(2) Enzymolysis: adding 10 times of water into the pulp of the pseudo-peach fruits, adding cellulose with the weight of 0.3% and the enzyme activity of 1000U/g into the pulp of the pseudo-peach fruits, carrying out enzymolysis for 2 hours at the constant temperature of 50 ℃ at the pH of=5.5, heating the enzymolysis liquid to 100 ℃ to inactivate for 10 minutes, and cooling to room temperature for standby;

(3) Microwave treatment: performing microwave treatment on the enzymolysis liquid in the step (2), wherein the microwave treatment conditions are as follows: the power is 400W, the temperature is 30 ℃ and the time is 5min;

(4) Fermentation: inoculating 1.5% by volume of Kluyveromyces lactis culture medium into the mixed solution obtained in the step (3), uniformly mixing, placing the mixture into a fermentation tank, and fermenting and culturing on a shaking table with the rotating speed of 120r/min for 5 days at the constant temperature of 35 ℃;

(5) Purifying: sterilizing the fermentation liquor in the step (4) at the high temperature of 121 ℃ for 30min, cooling, centrifuging to obtain a supernatant, loading the supernatant onto an H-60 macroporous adsorption resin column, wherein the loading amount is 4 times of the volume of the column, the loading flow rate is 1.0mL/min, standing for 1H after loading is finished, eluting with water with the volume of 4 times of the column, discarding the eluent, eluting with an ethanol solution with the volume concentration of 55% of 5.5 times of the column, the eluting rate is 0.5BV/H, recovering the eluent, and concentrating under reduced pressure to dryness to obtain the myrtle fruit extract.

Example 7

The invention provides a composition for improving skin wrinkles, which comprises the following components in parts by weight: 6g of ginseng root extract, 4g of rosewood heart wood fruit extract, 3g of sphingomonas fermentation product extract, 2g of sleeping vegetable leaf extract, 4g of ground tea leaf extract and 1g of hydrolyzed yeast protein.

Example 8

The invention provides a composition for improving skin wrinkles, which comprises the following components in parts by weight: 5g of ginseng root extract, 5g of rosewood heart wood fruit extract, 3g of sphingomonas fermentation product extract, 2g of sleeping vegetable leaf extract, 4g of ground tea leaf extract and 1g of hydrolyzed yeast protein.

The extract of the Pelargonium gracilis in examples 7-8 was prepared by the following steps in order:

(2) Enzymolysis: adding 10 times of water into the pulp of the pseudo-peach fruits, adding cellulose with the weight of 0.3% and the enzyme activity of 1000U/g into the pulp of the pseudo-peach fruits, carrying out enzymolysis for 2 hours at the constant temperature of 45 ℃ at the pH of=5.0, heating the enzymolysis liquid to 100 ℃ to inactivate for 10 minutes, and cooling to room temperature for standby;

(3) Microwave treatment: performing microwave treatment on the enzymolysis liquid in the step (2), wherein the microwave treatment conditions are as follows: the power is 300W, the temperature is 30 ℃ and the time is 4min;

(4) Fermentation: inoculating 1.2% by volume of Kluyveromyces lactis culture medium into the mixed solution obtained in the step (3), uniformly mixing, placing the mixture into a fermentation tank, and fermenting and culturing on a shaking table with the rotating speed of 120r/min for 4 days at the constant temperature of 35 ℃;

(5) Purifying: sterilizing the fermentation liquor in the step (4) at the high temperature of 121 ℃ for 30min, cooling, centrifuging to obtain a supernatant, loading the supernatant onto an H-60 macroporous adsorption resin column, wherein the loading amount is 4 times of the volume of the column, the loading flow rate is 1.0mL/min, standing for 1H after loading is finished, eluting with 4 times of the volume of the column, discarding the eluent, eluting with 6 times of the volume of 50% ethanol solution, the eluting rate is 1.0BV/H, recovering the eluent, and concentrating under reduced pressure to dryness to obtain the extract of the myrtle fruit.

The ginseng root extract described in examples 3-8 is an extract of black ginseng root.

Methods of using the skin wrinkle improvement compositions of any of the above examples 1-8: the components were weighed according to any one of the weight numbers of examples 1-8, mixed with a proper amount of water and polyol to 50g, and prepared as follows:

(1) Mixing sodium benzoate with small amount of water, and keeping the mixture for use (when sodium benzoate is not present, the step is omitted);

(2) Mixing phenoxyethanol with a small amount of polyalcohol in advance for standby (when phenoxyethanol is not present, the step is omitted);

(3) Heating the residual water to 40-45 ℃, adding the residual polyol, stirring uniformly, adding the pretreated solution obtained in the step (1) and the pretreated solution obtained in the step (2), stirring uniformly, and adding other materials, and stirring uniformly to obtain the modified polyurethane foam.

The above polyol may preferably be one or more of glycerin, butylene glycol, 1, 3-propanediol, 1, 2-pentanediol, propylene glycol, etc.

In order to demonstrate the effect of the present invention, comparative experimental examples of the present application are given below:

comparative example 1

In contrast to example 2, only the ginseng root extract was replaced with the equivalent amount of the tea extract, and the other components were the same.

The preparation method of the extract of the pseudo-myrtle is the same as that of example 2.

Comparative example 2

In comparison with example 2, only the sleeper leaf extract was replaced with an equivalent amount of hydrolyzed yeast protein, and the other components were the same.

Comparative example 3

In contrast to example 2, the raw material composition of the skin wrinkle-improving composition was the same.

Wherein the extract of the pseudo-myrtle is purchased through commercial channels and purchased from a Yusheng technology (International) group.

Comparative example 4

In contrast to example 3, the raw material composition of the skin wrinkle-improving composition was the same.

Wherein the extract of the pseudo-myrtle is prepared by the following steps in sequence:

(4) Purifying: and (3) cooling the mixture in the step (3), centrifuging to obtain a supernatant, loading the supernatant onto an H-60 macroporous adsorption resin column, wherein the loading amount is 3 times of the volume of the column, the loading flow rate is 1.0mL/min, standing for 1H after loading is finished, eluting with water with the volume of 4 times of the column, discarding the eluent, eluting with an ethanol solution with the volume concentration of 5 times of the column of 60%, eluting at the rate of 1.0BV/H, recovering the eluent, and concentrating under reduced pressure to dryness to obtain the extract of the pseudo-myrtle fruit.

Comparative example 5

A composition for improving skin wrinkles consists of the following components in parts by weight: 12g of ginseng root extract, 8g of rosewood heart wood fruit extract, 3g of sphingomonas fermentation product extract, 2g of sleeping vegetable leaf extract, 4g of ground tea leaf extract and 1g of hydrolyzed yeast protein.

The extract of the pseudo-myrtle is prepared by the following steps in sequence:

Milk as described in step (4)The concentration of kluyveromyces acidi is: 10 ⁷ CFU/mL, the culture medium in the step (4) comprises the following components: 10.0g of peptone, 8.0g of beef powder, 5.0g of yeast powder, 20.0g of glucose, 801.0mL of tween, 2.0g of dipotassium hydrogen phosphate, 5.0g of sodium acetate, 2.0g of diammonium hydrogen citrate, 0.5g of magnesium sulfate and 0.05g of manganese sulfate, and the volume is fixed to 1000mL by deionized water.

The ginseng root extract in comparative example 5 is an extract of black ginseng root.

The methods of use and preparation of any of the compositions of comparative examples 1-5 were the same as in the examples.

The composition for improving wrinkles can be used as an active ingredient for preparing cosmetics for improving wrinkles and delaying skin aging. The cosmetic can also comprise common matrix and/or deionized water of external preparation, and mainly comprises emulsifying agent, oil, emollient, humectant, thickener, pH regulator, polyalcohol, antiseptic, pigment, essence, etc. When the composition is specifically used, the composition prepared by the invention is directly added into a cosmetic matrix, and the addition amount is 0.1-6wt%.

To better illustrate the advantages of the present invention, the efficacy experiments of the wrinkle-improving composition provided by the present invention are given below:

test 1: skin irritation test

(1) Test sample: the compositions prepared in examples 1-8.

(2) The testing method comprises the following steps: 30 healthy volunteers were selected, aged 18-45 years, subjects should meet the volunteer selection criteria, no history of allergic dermatitis, and no irritating products were used in the previous month.

About 0.020-0.025 ml of the test sample is taken and coated in a small chamber of a spot tester (Beijing science instruments factory), a special adhesive tape for external application is applied on the back of a subject, the spot tester is removed after 24 hours, if the residual product is gently wiped off by a wet paper towel, the results are recorded according to the skin reaction grading standard in cosmetic safety technical Specification (2015) after 0.5 hour, 24 hours and 48 hours respectively. The identification criteria are shown in Table 1.

Table 1 identification criteria

Grade	Authentication criteria
		0	No irritation and erythema
1	Mild erythema
		2	Erythema (red spot)
3	Erythema, papule and bleb
		4	Severe edema and large foam

As shown by the test results of the closed patch, 30 subjects did not show any adverse reaction to the compositions for improving skin wrinkles prepared in examples 1 to 8 at 0.5 hours, 24 hours and 48 hours, and did not show symptoms such as erythema, burning sensation, itching and the like. The composition of the invention is safe and mild in use and has no irritation to skin.

Test 2: free radical scavenging capability test

This experiment evaluates the free radical scavenging properties of the compositions of the present invention by measuring the scavenging ability of the compositions to DPPH free radicals. DPPH radicals are very stable radicals, having a strong absorption at 517nm, and when encountering a radical scavenger, single electrons of DPPH are paired to lighten the color of the solution, the degree of which reflects the antioxidant activity.

(1) Test sample: examples 1-8, compositions prepared in comparative examples 1-5 were diluted with deionized water to a 3% mass fraction of the test solution.

(2) The testing method comprises the following steps: taking 3mL of DPPH-absolute ethyl alcohol solution with the concentration of 0.2mmol/L in a cuvette, adding 1mL of test sample, mixing uniformly, keeping away from light at room temperature for 30min, measuring absorbance at 517nm wavelength, marking the absorbance value as A1, adding 3mL of absolute ethyl alcohol solution and 1mL of test sample solution, marking the absorbance value as A2, and adding 3mL of PPH-absolute ethyl alcohol solution and 1mL of absolute ethyl alcohol solution, marking the absorbance value as A3. DPPH radical scavenging was calculated as follows: DPPH radical clearance (%) = [1- (A1-A2)/A3 ] ×100%, the average of three measurements was taken. The test results are shown in Table 2.

TABLE 2 DPPH free radical scavenging ability test results

As shown by the experimental results in Table 2, the composition has stronger scavenging ability to DPPH free radical and better scavenging effect than the composition of comparative example, which shows that the composition of the invention has synergistic effect, can improve the scavenging ability of free radical, increase oxidation resistance, reduce and avoid damage to organism caused by free radical, thereby achieving the purpose of delaying organism aging. In particular, the composition of example 7 showed the maximum DPPH radical scavenging rate, indicating that the composition was best effective when the ginseng root extract and the rosewood heart wood fruit extract were compounded in a certain ratio. Comparative example 3 and example 2, and comparative example 4 and example 3, which have less DPPH radical scavenging ability than the corresponding examples, demonstrate that the extract of Pelargonium graveolens can enhance its antioxidant properties by extraction using the preparation method of the present invention.

Test 3: wrinkle improvement efficacy test

(1) Test sample: the compositions prepared in examples 2, 3, 7, 8 and comparative examples 1 to 5 were added to the following base materials of essence in an amount of 3wt% respectively, to obtain cosmetics containing the compositions of examples 2, 3, 7, 8 and comparative examples 1 to 5, which were named as essences 1 to 4, comparative essences 1 to 5 in this order, and the base materials of essence were used as controls.

Phase A: water was added to 100g, phenoxyethanol// ethylhexyl glycerol 0.5g, disodium EDTA 0.03g;

and B phase: 5g of 1, 3-propanediol, 0.3g of carbomer, 0.05g of hydroxyethyl cellulose// sodium acetate// water// cellulose, 0.1g of xanthan gum and 0.12g of methylparaben;

and C phase: 1.5g of water and 0.07g of sodium hydroxide;

and D phase: 3g of butanediol and 0.5g of betaine;

e phase: 0.08g of sodium citrate, 0.04g of citric acid and 3g of water;

and F phase: 3g of the composition prepared in examples 2, 3, 7 and 8 or comparative examples 1-5, 0.06g of PEG-40 hydrogenated castor oil and a proper amount of essence.

The preparation methods of the essence 1-4 and the contrast essence 1-5 are as follows:

adding the phase A material into a vacuum emulsifying pot, stirring and heating to 85 ℃, adding the phase B material, mixing and stirring uniformly, preserving heat and stirring for 20 minutes, cooling to 60 ℃, adding the phase C material, stirring uniformly, continuing cooling to 50 ℃, adding the premixed phase D material, stirring uniformly, cooling to 45 ℃, adding the phase E material and the phase F material, stirring uniformly, cooling to 40 ℃, and discharging after the plates are qualified.

(2) The testing method comprises the following steps: 50 volunteers with average ages of 25-45 years and healthy skin and without damage were selected and randomly divided into 10 groups of 5 volunteers each, and the test samples were used. Each group of subjects takes 0.6g of essence to be smeared on the face each time, and each time is smeared once a day in the morning and evening, and the subjects are continuously used for 4 weeks. During the experiment, the subject was unable to apply any other cosmetics at the experimental site.

The skin elastometer CutomerMPA 580 was used to collect data when not in use and at week 4, the test site was the cheekbone, and the probe was repeatedly measured 3 times in the same region during measurement to obtain an average. The test parameter is R2 (ratio of rebound quantity Ua of skin without negative pressure to maximum stretching quantity Uf with negative pressure), the closer R2 is to 1, the better the elasticity of skin, the average change value of R2=the value of R2 at 4 th week-the value of R2 at 0 th week, the average change rate of R2=the average change value of R2/the value of R2 at 0 th week. The experimental results are shown in Table 4. The measurement results are shown in Table 3.

TABLE 3 wrinkle improvement efficacy test results

From the experimental results in table 3, after the essence prepared by the composition is continuously used for 4 weeks, the change rate of the skin elasticity R2 value reaches more than 15%, which is obviously better than the effect of the essence of the comparative example on improving the skin elasticity, so that the effects of improving wrinkles and delaying skin aging are finally achieved through reasonable compounding of ginseng extract, rosewood heart wood extract, sphingomonas campestris fermentation product extract, sleeping vegetable leaf extract and hydrolyzed yeast protein. When the ginseng root extract is selected from black ginseng root and the ginseng root extract and the rosewood heart wood fruit extract act at a specific ratio, the effect of the composition on improving skin elasticity is more remarkable.

The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.

Claims

1. A composition for improving skin wrinkles, which is characterized by comprising the following components in parts by weight: 5-10 parts of black ginseng root extract, 2-7 parts of myrtle fruit extract, 2-6 parts of sphingosine monad fermentation product extract, 0.5-3 parts of sleeper leaf extract, 2-4 parts of ground tea leaf extract, 1-4 parts of hydrolyzed yeast protein,

the preparation method of the pseudo-myrtle fruit extract comprises the following steps:

(4) Fermentation: inoculating a kluyveromyces lactis culture medium with the volume ratio of 0.5-1.5% into the mixed solution in the step (3), wherein the culture medium comprises the following components: 10.0g of peptone, 8.0g of beef powder, 5.0g of yeast powder, 20.0g of glucose, 1.0mL of Tween 80, 2.0g of dipotassium hydrogen phosphate, 5.0g of sodium acetate, 2.0g of diammonium hydrogen citrate, 0.5g of magnesium sulfate and 0.05g of manganese sulfate, and the volume is fixed to 1000mL by deionized water, wherein the concentration of the Kluyveromyces lactis in the culture medium is as follows: 10 ⁷ -10 ⁸ After being uniformly mixed, CFU/mL is placed in a fermentation tank and is fermented and cultured for 3-5 days on a shaking table with the rotating speed of 100-150r/min under the constant temperature condition of 30-35 ℃;

2. The composition for improving skin wrinkles according to claim 1, wherein the weight ratio of said black ginseng root extract to said extract of myrtus tomentosa is 3:2.

3. A cosmetic comprising the composition for improving skin wrinkles according to any one of claims 1 to 2 and a usual base of a cosmetic external dosage form.

4. The cosmetic product according to claim 3, wherein the usual base of the cosmetic external dosage form comprises one or more of an emulsifier, an emollient, a humectant, a thickener, a pH adjuster, a polyol, a preservative, a pigment and a fragrance.