CN113786379A - Lactic acid bacteria whitening and repairing essence and preparation method thereof - Google Patents
Lactic acid bacteria whitening and repairing essence and preparation method thereof Download PDFInfo
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- CN113786379A CN113786379A CN202111187327.0A CN202111187327A CN113786379A CN 113786379 A CN113786379 A CN 113786379A CN 202111187327 A CN202111187327 A CN 202111187327A CN 113786379 A CN113786379 A CN 113786379A
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- lactobacillus
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- skin
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Abstract
The invention discloses a lactobacillus whitening repair essence which comprises raw materials of lactobacillus/soybean milk fermentation product filtrate, lactobacillus fermentation lysate, schizosaccharomyces cerevisiae fermentation product lysate, saccharomycete/Sichuan valley seed fermentation product filtrate, trehalose, levan, tocopherol, sodium hyaluronate, a pinocembrus tomentosa extract, a grape seed extract, resveratrol, creatine, polyglutamic acid, xanthan gum, phenoxyethanol and ethylhexyl glycerol. The invention also discloses a preparation method of the lactobacillus whitening and repairing essence. The lactobacillus whitening and repairing essence disclosed by the invention can establish healthy micro-ecology of skin, strengthen the microbial barrier of the skin, simultaneously whiten the skin, soften cutin, be fine and smooth, keep moisture and be stable, promote cell metabolism, promote skin renewal and inhibit melanin activity.
Description
Technical Field
The invention relates to the field of beauty and skin care products, and particularly relates to lactic acid bacteria whitening and repairing essence and a preparation method thereof.
Background
Along with the continuous improvement of the quality of life, the market is more and more concerned about the demands of the whitening cosmetics, and various whitening cosmetics are full of the scope of the Lin Lang. At present, most of the whitening principles of the whitening cosmetics on the market aim at melanin which causes skin pigmentation and darkness, and the whitening cosmetics can reduce the generation of the melanin by inhibiting the activity of tyrosinase, or can achieve the effects of whitening and brightening the skin by carrying out catabolism and reduction on the generated melanin and blocking the generated melanin from migrating to the surface layer of the skin. The main skin whitening components in the skin care product mainly comprise vitamin C, arbutin, nicotinamide, kojic acid, tartaric acid and the like. However, skin color is often influenced by both intrinsic factors and extrinsic environmental factors, and whitening cosmetics on the market are often unsatisfactory in effect and risky in safety.
Disclosure of Invention
The invention aims to provide the probiotic whitening and repairing essence which is safe in components and can effectively whiten skin.
The invention also aims to provide a preparation method of the probiotic whitening and repairing essence.
In order to solve the problems, the invention provides a lactic acid bacteria whitening and repairing essence which comprises the following raw materials in parts by weight: 2-3 parts of lactobacillus/soybean milk fermentation product filtrate, 2-3 parts of lactobacillus fermentation lysate, 2-3 parts of schizosaccharomyces fermentation product lysate, 5-10 parts of yeast/cuckoo (COIX LACRYMA-JOBI MA-YUEN) seed fermentation product filtrate, 3-5 parts of trehalose, 2-3 parts of fructan, 1-2 parts of tocopherol (vitamin E), 0.2-0.5 part of sodium hyaluronate, 2-3 parts of CODIUM TOMENTOSUM (coumdium dinosum) extract, 2-3 parts of grape (VITIS VINIFERA) seed extract, 0.1-0.5 part of resveratrol, 0.5-1 part of creatine, 0.2-0.5 part of polyglutamic acid, 0.1-0.2 part of xanthan gum, 0.2-0.3 part of phenoxyethanol and 0.5-1 part of ethylhexylglycerin.
Preferably, the lactobacillus whitening and repairing essence further comprises 100-150 parts by weight of deionized water.
The invention also provides a method for preparing the lactobacillus whitening and repairing essence, which comprises the following steps:
(1) sequentially adding trehalose, fructan, creatine and polyglutamic acid into deionized water, uniformly dispersing, sequentially adding sodium hyaluronate and xanthan gum, and performing ultrasonic treatment under the condition of heating to obtain a first mixture;
(2) adjusting the temperature of the first mixture to 55-60 ℃, adding tocopherol, and performing ultrasonic treatment to obtain a second mixture;
(3) adjusting the temperature of the second mixture to below 50 ℃, adding lactobacillus/soybean milk fermentation product filtrate, lactobacillus fermentation lysate, yeast/Sichuan valley seed fermentation product filtrate, pinus hirsutus extract, grape seed extract and resveratrol, and performing ultrasonic treatment to obtain a third mixture;
(4) and adjusting the temperature of the third mixture to 40-45 ℃, adding phenoxyethanol and ethylhexylglycerin, performing ultrasonic treatment, and cooling to 20-35 ℃ to obtain the lactobacillus whitening and repairing essence.
Preferably, in the step (1), the ultrasonic treatment in the heating process is carried out at a temperature of 80 to 90 ℃ and an ultrasonic frequency of 30 to 50kHz for 20 to 30 minutes.
In the step (2), the ultrasonic frequency is 30-50 kHz, and the ultrasonic time is 10-20 min;
in the step (3), the ultrasonic frequency is 40-80 kHz, and the ultrasonic time is 10-20 min.
In the step (4), the ultrasonic frequency is 40-80 kHz, and the ultrasonic time is 10-20 min.
The steps (1) to (4) are carried out in an emulsifying pot.
The lysate of fermentation product of yeast BIFIDA (BIFIDA FERMENT LYSATE, CAS 96507-89-0) is metabolite, cytoplasmic fragment, cell wall component and polysaccharide complex obtained by culturing, inactivating and decomposing Bacillus bifidus. The extraction process is similar to that of yeast fermentation product lysate, and has strong immunosuppressive activity, and can promote DNA repair and protect skin from ultraviolet ray damage. The yeast secondary fission fermentation product lysate comprises micromolecules such as vitamin B group, mineral substances, amino acids and the like which are beneficial to skin care, is high-quality yeast essence which can be used for skin care, can strengthen the metabolism of cuticle, can capture free radicals, inhibits the peroxidation of lipid, has the functions of whitening and resisting aging, is rich in nutrient substances, and has the function of nourishing the skin.
The lactobacillus fermentation lysate contains thalli and all active ingredients synthesized in the fermentation process, contains peptidoglycan, phospholipid, sterol, fatty acid, exopolysaccharide and the like, can provide rich nutrition for skin, and has good anti-aging effect.
The yeast/fructus Sichuan grain (COIX LACRYMA-JOBI MA-YUEN) seed fermentation product filtrate is mixed fermentation product filtrate of yeast and Coicis semen, and has effects of increasing skin activity, reducing wrinkle, and resisting aging; is commonly used as a skin conditioner in skin care products.
The lactobacillus/soybean milk fermentation product filtrate is black soybean milk filtrate obtained by fermenting 16 35 strains of lactobacillus, is rich in various vitamins, proteins and active ingredients, and has the effects of protecting, relieving, moisturizing, repairing and whitening.
The sodium hyaluronate is in the form of sodium salt of hyaluronic acid, is a high molecular weight linear polysaccharide, is widely distributed in animal and human connective tissue extracellular matrixes, and has good viscoelasticity, plasticity and permeability and good biocompatibility. The natural moisturizing agent is widely used in the production of cosmetics, plays a unique role as a moisturizing factor in the cosmetics, has a higher moisturizing effect compared with the traditional moisturizing agent, and has no defects of greasiness, blockage of skin pores and the like. When the sodium hyaluronate is added into cosmetics and is coated on the surface of skin, a viscoelastic transparent hydrated film can be formed, the film HAs good water retention effect, just like HA naturally existing in stem cell interstitium, the film can increase the water content of the skin stratum corneum, and the water content of the stratum corneum is closely related to the maintenance of skin health and defense function to external stimulation. The sodium hyaluronate can also permeate into the dermis to regulate the metabolism of the skin, thereby achieving the effects of wrinkle resistance, beautifying and health care.
The Arthodium hirsutum extract contains abundant vitamins and phenols, has strong moisturizing capability, and can effectively increase the moisturizing capability of skin.
Resveratrol is a non-flavonoid polyphenol compound with a stilbene structure and strong biological activity, mainly comes from plants such as grapes, giant knotweed, peanuts, mulberries and the like, has some commonness of plant polyphenol and unique biological activity. Resveratrol has obvious effects of resisting oxidation and free radicals, and has good effect on preventing and treating oxidative stress related to aging; resveratrol has good inhibitory effect on tyrosinase in B16 cells, can reduce AGEs generation amount, inhibit protein carbonylation, reduce content of Advanced Oxidation Protein Product (AOPP), and has anti-glycation effect, and also has antiinflammatory, antibacterial and moisturizing effects.
The extract of grape (VITIS VINIFERA) seed has effect in inhibiting caspase-8, which is the core component of cell apoptosis, and inhibiting it can prolong cell life, enhance skin activity, eliminate free radicals, and inhibit the production of collagenase and nitrogen oxide. In addition, at a concentration of 1%, the grape (VITIS VINIFERA) seed extract has effects in inhibiting melanin generation, shrinking and astringing pore diameter, and tightening skin.
The sodium polyglutamate has strong moisture retention capacity, can effectively increase the moisture retention capacity of skin, can resist wrinkles for a long time, and can be used as a whitening agent.
Has the advantages that: the probiotic whitening and repairing essence disclosed by the invention can establish healthy micro-ecology of skin, strengthen the microbial barrier of the skin, simultaneously whiten the skin, soften cutin, be fine and smooth, keep moisture and be stable, and can promote cell metabolism, promote skin renewal and inhibit melanin activity.
Detailed Description
Example 1
The preparation of the lactobacillus whitening and repairing essence comprises the following steps:
(1) adding 1kg of deionized water into an emulsifying pot, sequentially adding 30g of trehalose, 20g of fructan, 5g of creatine and 2g of polyglutamic acid, uniformly dispersing, sequentially adding 2g of sodium hyaluronate and 1g of xanthan gum, and carrying out ultrasonic treatment at the temperature of 80 ℃ and the ultrasonic frequency of 40kHz for 25 minutes to obtain a first mixture;
(2) adjusting the temperature of the first mixture to 60 ℃, adding 10g of tocopherol, and carrying out ultrasonic treatment for 15 minutes at the ultrasonic frequency of 40kHz to obtain a second mixture;
(3) adjusting the temperature of the second mixture to 45 ℃, adding 30g of lactobacillus/soybean milk fermentation product filtrate, 20g of lactobacillus fermentation lysate, 20g of saccharomyces cerevisiae fermentation product lysate, 50g of saccharomycetes/Sichuan millet seed fermentation product filtrate, 20g of pinocembrus tomentosa extract, 20g of grape seed extract and 1g of resveratrol, and carrying out ultrasonic treatment for 15 minutes at the ultrasonic frequency of 60kHz to obtain a third mixture;
(4) and adjusting the temperature of the third mixture to 40 ℃, adding 2g of phenoxyethanol and 5g of ethylhexyl glycerol, carrying out ultrasonic treatment for 20 minutes at the ultrasonic frequency of 40kHz, and cooling to 25 ℃ to obtain the lactobacillus whitening and repairing essence.
Example 2
The preparation of the lactobacillus whitening and repairing essence comprises the following steps:
(1) adding 1.5kg of deionized water into an emulsifying pot, then sequentially adding 50g of trehalose, 30g of fructan, 10g of creatine and 5g of polyglutamic acid, after uniform dispersion, sequentially adding 5g of sodium hyaluronate and 2g of xanthan gum, and carrying out ultrasonic treatment at 90 ℃ and the ultrasonic frequency of 50kHz for 20 minutes to obtain a first mixture;
(2) adjusting the temperature of the first mixture to 55 ℃, adding 20g of tocopherol, and carrying out ultrasonic treatment for 10 minutes at an ultrasonic frequency of 50kHz to obtain a second mixture;
(3) adjusting the temperature of the second mixture to 50 ℃, adding 20g of lactobacillus/soybean milk fermentation product filtrate, 30g of lactobacillus fermentation lysate, 30g of saccharomyces cerevisiae fermentation product lysate, 100g of saccharomycetes/Sichuan millet seed fermentation product filtrate, 30g of pinocembrus tomentosa extract, 30g of grape seed extract and 5g of resveratrol, and carrying out ultrasonic treatment for 10 minutes at the ultrasonic frequency of 80kHz to obtain a third mixture;
(4) and adjusting the temperature of the third mixture to 45 ℃, adding 3g of phenoxyethanol and 10g of ethylhexyl glycerol, carrying out ultrasonic treatment for 10 minutes at the ultrasonic frequency of 80kHz, and cooling to 20 ℃ to obtain the lactobacillus whitening and repairing essence.
Example 3
The preparation of the lactobacillus whitening and repairing essence comprises the following steps:
(1) adding 1.2kg of deionized water into an emulsifying pot, then sequentially adding 40g of trehalose, 25g of fructan, 7g of creatine and 3g of polyglutamic acid, after uniform dispersion, sequentially adding 3g of sodium hyaluronate and 1.5g of xanthan gum, and carrying out ultrasonic treatment at the ultrasonic frequency of 30kHz for 30 minutes at the temperature of 85 ℃ to obtain a first mixture;
(2) adjusting the temperature of the first mixture to 55 ℃, adding 20g of tocopherol, and carrying out ultrasonic treatment for 20 minutes at the ultrasonic frequency of 30kHz to obtain a second mixture;
(3) adjusting the temperature of the second mixture to 45 ℃, adding 25g of lactobacillus/soybean milk fermentation product filtrate, 25g of lactobacillus fermentation lysate, 25g of saccharomyces cerevisiae fermentation product lysate, 70g of saccharomycetes/Sichuan millet seed fermentation product filtrate, 25g of pinocembrus tomentosa extract, 25g of grape seed extract and 3g of resveratrol, and carrying out ultrasonic treatment for 20 minutes at the ultrasonic frequency of 40kHz to obtain a third mixture;
(4) and adjusting the temperature of the third mixture to 40 ℃, adding 2.5g of phenoxyethanol and 7g of ethylhexyl glycerol, carrying out ultrasonic treatment for 15 minutes at the ultrasonic frequency of 60kHz, and cooling to 35 ℃ to obtain the lactobacillus whitening and repairing essence.
Comparative example 1
The trehalose in example 1 was replaced with deionized water, and the other conditions were unchanged to obtain the sample of comparative example 1.
Comparative example 2
The fructan of example 1 was replaced with deionized water, and the other conditions were unchanged to give the sample of comparative example 2.
Comparative example 3
The creatine in example 1 was replaced with deionized water and other conditions were unchanged to give the sample of comparative example 3.
Comparative example 4
The polyglutamic acid in example 1 was replaced with deionized water, and other conditions were unchanged to obtain a sample of comparative example 4.
Comparative example 5
The sodium hyaluronate from example 1 was replaced with deionized water and the other conditions were unchanged to obtain a sample of comparative example 5.
Comparative example 6
The xanthan gum of example 1 was replaced with deionized water and the other conditions were unchanged to give the sample of comparative example 6.
Comparative example 7
The tocopherol of example 1 was replaced with deionized water and the other conditions were unchanged to give the sample of comparative example 7.
Comparative example 8
The lactobacillus/soybean milk fermentation product filtrate of example 1 was replaced with deionized water, and the other conditions were not changed to obtain a sample of comparative example 8.
Comparative example 9
The example 1 in lactic acid bacteria fermentation lysate was replaced with deionized water, other conditions were not changed, and the sample of comparative example 9 was obtained.
Comparative example 10
The two split yeast fermentation product lysate in example 1 was replaced with deionized water, other conditions were unchanged, and the sample of comparative example 10 was obtained.
Comparative example 11
The yeast/chuangu seed fermentation product filtrate in example 1 was replaced with deionized water, and the other conditions were unchanged to obtain a sample of comparative example 11.
Comparative example 12
The Arthodium hirsutum extract of example 1 was replaced with deionized water under otherwise unchanged conditions to obtain a sample of comparative example 12.
Comparative example 13
The grape seed extract of example 1 was replaced with deionized water and the other conditions were unchanged to obtain the sample of comparative example 13.
Comparative example 14
The resveratrol from example 1 was replaced with deionized water and the other conditions were unchanged to give the sample of comparative example 14.
Comparative example 15
The phenoxyethanol of example 1 was replaced with deionized water under otherwise unchanged conditions to provide a sample of comparative example 15.
Comparative example 16
The ethylhexyl glycerin obtained in example 1 was replaced with deionized water under otherwise unchanged conditions to obtain a sample of comparative example 16.
Example 4
Randomly selecting 120 volunteers between 18-55 years old, wherein the number of the volunteers is the same as that of the volunteers, and the volunteers need to meet the following conditions:
(a) no serious systemic disease, no immunodeficiency or autoimmune disease
(b) The past has no history of allergy to skin care cosmetics;
(c) an inactive allergic disease;
(d) hormonal drugs and immunosuppressive agents have not been used systemically within one month;
(e) outdoor work is not taken as a main part, and outdoor activities exceeding the conventional amount are not performed during the test period;
(f) whitening products (including other cosmetics, external medicines or internal health care products) cannot be used 3 days before the test.
The volunteers are randomly divided into 20 groups on the premise that the number of men and women in each group is the same, 6 people in each group are selected, 5mL of the lactobacillus whitening and repairing essence prepared in the embodiment 1-3, the comparative examples 1-16 prepared in the comparative examples 1-16 and deionized water are uniformly smeared on the face every morning, noon and evening, and the test lasts for 28 days. No other skin care or cosmetic products, etc. were used during the test period.
(1) Melanin content test
Each volunteer tested the amount of melanin in the skin before the test was performed on day 1 and 2 hours after the test was performed on day 28 using a skin elasticity Cotomer MPA580 host manufactured by Courage + Khazaka, Germany, and a skin melanin Mexamer MX18 test probe manufactured by CK, Germany. The melanin content is carried out indoors, strong sunlight or direct light is avoided, the ambient temperature is 20 ℃, and the humidity is 40-60%. The volunteers were uniformly cleansed of facial skin and wiped dry with facial tissue prior to testing. The facial test area is the middle point position of the connecting line between the nose tip and the outer side of the left eye pupil.
The melanin content of each group of subjects was averaged and the results are shown in table 1.
TABLE 1 test results for melanin content
As can be seen from the data in Table 1, the skin melanin content of the volunteers using the lactobacillus whitening and repairing essence is obviously reduced, which shows that the lactobacillus whitening and repairing essence has better whitening effect.
(2) Skin elasticity test
Each volunteer tested skin elasticity before the test was performed on day 1 and 2 hours after the test was performed on day 28 using a skin elasticity Cotometer MPA580 master manufactured by Courage + Khazaka, germany, a skin elasticity PVM600 test probe manufactured by CK, germany. The skin elasticity test is carried out indoors, strong sunlight or direct light does not exist, the ambient temperature is 20 ℃, and the humidity is 40-60%. The volunteers were uniformly cleansed of facial skin and wiped dry with facial tissue prior to testing. The facial test area is the middle point position of the connecting line between the nose tip and the outer side of the left eye pupil.
Skin elasticity test instrument parameter setting: negative pressure-450 mbar, negative pressure time 2 s. The evaluation method comprises the following steps: the skin elasticity recovery parameter R2 is Ua/Uf, and the closer the R2 data is to 1, the better the skin elasticity is, the ratio of the amount of springback of the skin (Ua) in the absence of negative pressure to the maximum amount of stretch in the presence of negative pressure (Uf). The R2 data were averaged for each group of subjects and the results are shown in table 2.
Table 2 skin elastic recovery parameter test results
As can be seen from the data in Table 2, the skin elasticity recovery parameters of the volunteers using the lactobacillus whitening and repairing essence are obviously improved, which shows that the lactobacillus whitening and repairing essence has a good effect of improving the skin elasticity.
(3) Skin moisture content test
Each volunteer tested skin elasticity before the test was conducted on day 1 and 2 hours after the test was conducted on day 28 using a skin elasticity Cotomer MPA580 host manufactured by Courage + Khazaka, Germany, and a skin moisture content Cornemeter CM825 test probe manufactured by CK, Germany. The skin elasticity test is carried out indoors, strong sunlight or direct light does not exist, the ambient temperature is 20 ℃, and the humidity is 40-60%. The volunteers were uniformly cleansed of facial skin and wiped dry with facial tissue prior to testing. The facial test area is the middle point position of the connecting line between the nose tip and the outer side of the left eye pupil.
The skin Moisture content test results are expressed by set Moisture Measurement Values (MMV): the skin moisture content Cornemeter CM825 test probe is based on the principle of capacitance. When the moisture content changes, the capacitance of the skin also changes, so the moisture content on the skin surface can be analyzed by measuring the capacitance of the skin. The measured value is a relative value, and the unit is represented by C.U (Corneometer Units). The larger the MMV value, the greater the skin moisture content, and conversely, the smaller the skin moisture content.
The MMV data for each group of subjects was averaged and the results are shown in Table 3.
Table 3 skin MMV test results
As can be seen from the data in Table 3, the skin moisture content of the volunteers using the lactobacillus whitening and repairing essence is obviously improved, which shows that the lactobacillus whitening and repairing essence has a good effect of improving the skin elasticity.
(4) By reviewing the volunteers of the trial groups of the lactic acid bacterium whitening and repairing essence of example 1, example 2 and example 3, 0 person showed problems of allergy, redness and swelling or other discomfort after using the lactic acid bacterium whitening and repairing essence of the present invention.
Claims (8)
1. The lactobacillus whitening and repairing essence is characterized by comprising the following raw materials in parts by weight: 2-3 parts of lactobacillus/soybean milk fermentation product filtrate, 2-3 parts of lactobacillus fermentation lysate, 2-3 parts of yeast schizolysis product, 5-10 parts of saccharomycetes/Sichuan valley seed fermentation product filtrate, 3-5 parts of trehalose, 2-3 parts of levan, 1-2 parts of tocopherol, 0.2-0.5 part of sodium hyaluronate, 2-3 parts of pinus hirsutus extract, 2-3 parts of grape seed extract, 0.1-0.5 part of resveratrol, 0.5-1 part of creatine, 0.2-0.5 part of polyglutamic acid, 0.1-0.2 part of xanthan gum, 0.2-0.3 part of phenoxyethanol and 0.5-1 part of ethylhexylglycerol.
2. The lactic acid bacteria whitening and repairing essence according to claim 1, further comprising 100-150 parts by weight of deionized water.
3. A method for preparing the lactic acid bacteria whitening and repairing essence of any one of claims 1-2, comprising the following steps:
(1) sequentially adding trehalose, fructan, creatine and polyglutamic acid into deionized water, uniformly dispersing, sequentially adding sodium hyaluronate and xanthan gum, and performing ultrasonic treatment under the condition of heating to obtain a first mixture;
(2) adjusting the temperature of the first mixture to 55-60 ℃, adding tocopherol, and performing ultrasonic treatment to obtain a second mixture;
(3) adjusting the temperature of the second mixture to be below 50 ℃, adding lactobacillus/soybean milk fermentation product filtrate, lactobacillus fermentation lysate, yeast/Sichuan valley seed fermentation product filtrate, pinocembrus tomentosa extract, grape seed extract and resveratrol, and performing ultrasonic treatment to obtain a third mixture;
(4) and adjusting the temperature of the third mixture to 40-45 ℃, adding phenoxyethanol and ethylhexylglycerin, performing ultrasonic treatment, and cooling to 20-35 ℃ to obtain the lactobacillus whitening and repairing essence.
4. The method according to claim 3, wherein in the step (1), the ultrasound under heating is performed at a temperature of 80-90 ℃ and an ultrasound frequency of 30-50 kHz for 20-30 minutes.
5. The method according to claim 3, wherein in the step (2), the frequency of the ultrasound is 30-50 kHz, and the ultrasound time is 10-20 min.
6. The method according to claim 3, wherein in the step (3), the frequency of the ultrasound is 40-80 kHz, and the ultrasound time is 10-20 min.
7. The method according to claim 3, wherein in the step (4), the frequency of the ultrasound is 40-80 kHz, and the ultrasound time is 10-20 min.
8. The method of claim 3, wherein steps (1) - (4) are carried out in an emulsifying kettle.
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| CN114459878A (en) * | 2022-01-21 | 2022-05-10 | 东北农业大学 | Method for improving gel characteristics of fermented soybean milk by using lactobacillus casei in combination with ultrasonic treatment |
| CN115581655A (en) * | 2022-04-13 | 2023-01-10 | 广州市万千粉丝化妆品有限公司 | Application and preparation of 3X nutrient and muscle energy maintenance interstitial fluid preparation |
| CN115725475A (en) * | 2022-12-15 | 2023-03-03 | 广州悦荟化妆品有限公司 | Symbiotic fermentation schizosaccharomyces cerevisiae lysate with effects of tightening, resisting wrinkles, resisting oxidation and repairing and preparation method thereof |
| CN115887259A (en) * | 2022-11-25 | 2023-04-04 | 上海臻臣化妆品有限公司 | Anti-inflammatory and anti-aging composition, anti-inflammatory and anti-aging essence and preparation method thereof |
| CN117051048A (en) * | 2022-12-15 | 2023-11-14 | 东莞华工创为生物科技有限公司 | Lysate of fermentation product of saccharomyces cerevisiae, and preparation method and application thereof |
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| CN115581655A (en) * | 2022-04-13 | 2023-01-10 | 广州市万千粉丝化妆品有限公司 | Application and preparation of 3X nutrient and muscle energy maintenance interstitial fluid preparation |
| CN115887259A (en) * | 2022-11-25 | 2023-04-04 | 上海臻臣化妆品有限公司 | Anti-inflammatory and anti-aging composition, anti-inflammatory and anti-aging essence and preparation method thereof |
| CN115725475A (en) * | 2022-12-15 | 2023-03-03 | 广州悦荟化妆品有限公司 | Symbiotic fermentation schizosaccharomyces cerevisiae lysate with effects of tightening, resisting wrinkles, resisting oxidation and repairing and preparation method thereof |
| CN115725475B (en) * | 2022-12-15 | 2023-08-15 | 广州悦荟化妆品有限公司 | Symbiotic fermentation two-split yeast lysate with effects of tightening, resisting wrinkles, resisting oxidization and repairing and preparation method thereof |
| CN117051048A (en) * | 2022-12-15 | 2023-11-14 | 东莞华工创为生物科技有限公司 | Lysate of fermentation product of saccharomyces cerevisiae, and preparation method and application thereof |
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