CN111407686A

CN111407686A - Multi-effect repair spray with vegetable protein and polypeptide formula and preparation method thereof

Info

Publication number: CN111407686A
Application number: CN202010466715.1A
Authority: CN
Inventors: 杨跃飞; 刘元柏
Original assignee: Shanghai Jieli Biotechnology Co ltd
Current assignee: Shanghai Jieli Biotechnology Co ltd
Priority date: 2020-05-28
Filing date: 2020-05-28
Publication date: 2020-07-14

Abstract

The invention relates to the field of cosmetic products for plastic medicine, in particular to a multi-effect repair spray for a plant protein and polypeptide formula and a preparation method thereof, wherein the multi-effect repair spray for the plant protein and polypeptide formula comprises, by weight, 5-20 parts of a humectant, 5-20 parts of a skin repair agent, 0.5-2.5 parts of an emulsion solubilizer and 100 parts of deionized water, wherein the skin repair agent comprises 3-6 parts of hydrolyzed plant protein, 0.2-1.6 parts of polypeptide, 0.5-1.5 parts of lactobacillus/soybean milk fermentation product filtrate and 1-3 parts of yeast β -glucan.

Description

Multi-effect repair spray with vegetable protein and polypeptide formula and preparation method thereof

Technical Field

The invention relates to the field of plastic medical cosmetics, in particular to a multi-effect repair spray with a vegetable protein and polypeptide formula and a preparation method thereof.

Background

The medical plastic surgery is pursued by young people due to higher technical specialty and good effect, so the number of professional plastic hospitals and plastic surgery departments is increased sharply, and the market demand is strong. The "plastic medical cosmetology" is a method for changing the appearance or skin of a human body by medical "surgical operation" means so as to achieve the purpose of shaping beauty and health. The 'surgical operation' means is different from the traditional 'life cosmetology' using external cosmetics, and relates to 'moving knife' on the macro scale, such as 'macro plastic' of face, eye, nose, mouth, chest and the like, and 'photoelectric operation' on the micro scale, such as 'micro-invasive plastic' of laser spot removing, photon skin tendering, radio frequency wrinkle removing and the like. "cosmetic medical cosmetology" has become the most important means of beauty and health in modern and future, occupying an ever-increasing cosmetic market. However, both macroscopic "surgery" and microscopic "photoelectric surgery" and "plastic medical cosmetology" have the problem of "skin damage", such as dehydration, dryness, redness, minimal invasive wound, etc., which cannot be solved well, greatly affect the effect of "plastic medical cosmetology", and thus urge the development of "plastic medical cosmetology" products for preoperative and postoperative care and recovery, i.e., "medical cosmetology" or "medical cosmetology". Practice proves that the medical cosmetic plays a vital role in preoperative and postoperative plastic medical cosmetology, and can achieve a better cosmetology effect. However, the number of repair products for minimally invasive plastic surgery in the market today is not large, and there are many drawbacks in the use of more specialized, more effective and more modern "biochemically active ingredients" in the technical formulation.

Disclosure of Invention

Aiming at some problems in the prior art, the invention provides a multi-effect repairing spray based on a modern 'biochemical active ingredient' plant protein and polypeptide formula, which comprises, by weight, 5-20 parts of a humectant, 5-20 parts of a skin repairing agent, 0.5-2.5 parts of an emulsification solubilizer and 100 parts of deionized water, wherein the skin repairing agent comprises 3-6 parts of hydrolyzed plant protein, 0.2-1.6 parts of polypeptide, 0.5-1.5 parts of lactobacillus/soybean milk fermentation product filtrate and 1-3 parts of yeast β -glucan.

According to a preferable technical scheme, the skin repairing agent further comprises, by weight, 0.1-1 part of ceramide, 0.1-1 part of allantoin and 1-5 parts of semen carolinae extract.

In a preferred embodiment of the present invention, the emulsification solubilizer is hydrogenated lecithin and/or hydrogenated castor oil.

According to a preferable technical scheme of the invention, the emulsification solubilizer comprises 0.3-1 part of hydrogenated lecithin and 0.5-1.5 parts of hydrogenated castor oil in parts by weight.

According to a preferable technical scheme of the invention, the polypeptide comprises, by weight, 0.1-1 part of carnosine, 0.05-0.2 part of tripeptide-1 copper, 0.05-0.15 part of oligopeptide-1 and 0.03-0.2 part of palmitoyl pentapeptide-4.

According to a preferable technical scheme, the humectant comprises 0.05-1 part by weight of sodium hyaluronate and 0.5-5 parts by weight of trehalose.

As a preferable technical scheme, the molecular weight of the sodium hyaluronate is less than or equal to 1 ten thousand daltons.

According to a preferable technical scheme, the humectant further comprises 2-5 parts by weight of glycerin, 0.5-3 parts by weight of 1, 2-pentanediol, 1-3 parts by weight of betaine and 1-3 parts by weight of saccharide isomers.

As a preferred technical scheme of the invention, the multiple-effect repair spray of the plant protein and polypeptide formula further comprises 0.1-1 part by weight of a composite chelating agent; the complex chelating agent comprises caprylyl hydroximic acid, hexanediol and 1, 3-propylene glycol.

The second aspect of the invention provides a preparation method of a multi-effect repair spray of a vegetable protein and polypeptide formula, which comprises the following steps:

(1) after the deionized water is subjected to heat preservation and sterilization at 85-90 ℃/30 minutes, the mixture is cooled to 60 ℃, added with the humectant and the emulsification solubilizer and fully stirred uniformly, and homogenized for 5-10 min;

(2) adding yeast β -dextran and lactobacillus/soybean milk fermentation product filtrate in skin repairing agent at 50 deg.C, stirring, mixing and dissolving;

(3) cooling to below 30 deg.C, adding the rest components, and stirring to dissolve.

Compared with the prior art, the invention has the following beneficial effects:

the multiple-effect repair spray of the vegetable protein and polypeptide formula provided by the invention is safe and non-irritant, has obvious effect on recovery of the skin after plastic surgery, overcomes the problem of poor repair effect of the existing repair products, and is particularly suitable for repair of minimally invasive plastic surgery of photoelectric surgery.

Drawings

FIG. 1 is a graph showing the moisturizing effects of the multi-effect remedial spray obtained in example 3 of the present invention and a control;

FIG. 2 is a graph showing the repairing effects of the multi-effect repairing spray obtained in example 3 of the present invention and a control on skin barrier damage;

FIG. 3 is a graph showing the effect of the multi-effect remedial spray obtained in example 3 of the present invention on improvement of skin elasticity and a control sample;

FIG. 4 is a graph showing the effect of the multi-effect conditioning spray of example 3 of the present invention on the improvement of fine lines and a control.

Detailed Description

The present invention is illustrated by the following specific embodiments, but is not limited to the specific examples given below.

The invention provides a modern 'biochemical active ingredient' based multi-effect repairing spray for a vegetable protein and polypeptide formula, which comprises, by weight, 5-20 parts of a humectant, 5-20 parts of a skin repairing agent, 0.5-2.5 parts of an emulsifying solubilizer and 100 parts of deionized water.

In one embodiment, the multi-effect repair spray of the plant protein and polypeptide formulation further comprises 0.1 to 1 part by weight of a complex chelating agent.

Moisture-retaining agent

In one embodiment, the humectant comprises 0.05 to 1 part by weight of sodium hyaluronate and 0.5 to 5 parts by weight of trehalose.

In one embodiment, the humectant further comprises 2-5 parts by weight of glycerin, 0.5-3 parts by weight of 1, 2-pentanediol, 1-3 parts by weight of betaine, and 1-3 parts by weight of saccharide isomers.

Preferably, the humectant comprises 0.5 part of sodium hyaluronate, 1 part of trehalose, 3 parts of glycerin, 1 part of 1, 2-pentanediol, 2 parts of betaine and 2 parts of saccharide isomers in parts by weight.

In one embodiment, the sodium hyaluronate has a molecular weight of less than or equal to 1 kilodalton.

The sodium hyaluronate is purchased from Shandong Huaxinfuruida biomedicine Limited company, the trehalose is purchased from Koshifa International trade (Shanghai) Limited company, and the saccharide isomer is produced by Stesmann Nutrients Limited company (DSMNTUTRITIIONA L PRODUCTS L TD), is sold as PENTAVITIN and is purchased from Shanghai Qiuqun Shanghai Shangfu Shanghai Shan.

The glycerin is a commonly used humectant for cosmetics of plant sources and also has a solvent, so that the solubility of the formula is increased.

The 1, 2-pentanediol in the invention is a safe humectant with bacteriostatic function, and can enhance the antiseptic property of the formula.

The betaine is extracted from beet root, mainly contains trimethylglycine, has long-acting moisturizing and anti-allergy repairing effects, provides sufficient water and nutrients for the horny layer, activates cells, and enhances moisturizing and barrier functions.

The saccharide isomerate of the invention is a complex saccharide substance derived from plant D-glucose, and the main components are glucose, fructose, galactose and the like. Because of its composition similar to that of the carbohydrate complex (natural moisturizing factor NMF) in human skin, it has important effects in constructing long-acting moisturizing mechanisms of skin and activating genes that play a key role in the barrier.

Skin repair agent

In one embodiment, the skin repairing agent comprises 3-6 parts by weight of hydrolyzed vegetable protein, 0.2-1.6 parts by weight of polypeptide, 0.5-1.5 parts by weight of lactobacillus/soybean milk fermentation product filtrate, and 1-3 parts by weight of yeast β -glucan.

In one embodiment, the skin repair agent further comprises 0.1-1 part of ceramide, 0.1-1 part of allantoin and 1-5 parts of semen carolinae extract by weight.

< hydrolysis of vegetable protein >

In one embodiment, the hydrolyzed vegetable protein comprises at least one of hydrolyzed soy protein, hydrolyzed corn protein, and hydrolyzed wheat protein.

Preferably, the hydrolyzed vegetable protein is hydrolyzed soy protein.

The hydrolyzed soy protein of the present invention is produced by Vincience, France, and is available from Shanghai Dry fly chemical Co., Ltd.

The lactobacillus/soybean milk fermentation product filtrate of the invention is produced by France AXIA L YS INVAVATIONS company and purchased from Shanghai Yan happy industry Co.

The yeast β -glucan of the present invention was produced by BIOSPETRUM, Inc. of Korea, and was purchased from Shanghai Risoku chemical technology, Inc.

The carob fruit extract of the present invention is produced by SI L AB, france (SI L AB s.a.) and is available from shanghai fangkai underwriting ltd.

< polypeptide >

In one embodiment, the polypeptide comprises 0.1-1 part of carnosine, 0.05-0.2 part of tripeptide-1 copper, 0.05-0.15 part of oligopeptide-1 and 0.03-0.2 part of palmitoyl pentapeptide-4 in parts by weight.

Preferably, the polypeptide comprises 0.2 parts of carnosine, 0.15 parts of tripeptide-1 copper, 0.1 parts of oligopeptide-1 and 0.07 parts of palmitoyl pentapeptide-4 in parts by weight.

The oligopeptide-1 is purchased from Angel biotechnology limited company in Shenzhen.

The palmitoyl pentapeptide-4 is purchased from Zhejiang surge tide biotechnology limited.

In a preferred embodiment, the skin repair agent comprises, by weight, 4.5 parts hydrolyzed vegetable protein, 0.52 parts polypeptide, 1.2 parts lactobacillus/soymilk fermentation product filtrate, 2 parts yeast β -dextran, 0.2 parts ceramide, 0.2 parts allantoin, 2 parts carob seed extract.

The ceramide is the main component of the sebum of the skin stratum corneum, is prepared by a yeast biochemical method, and has the effects of maintaining the moisture balance of the skin barrier and strengthening the barrier function revival of dry skin and aged skin.

The allantoin is a skin anti-inflammatory and anti-drying component commonly used in medicines and cosmetics, has the effects of well softening cutin, promoting cell growth, repairing damage caused by environment and mechanical pressure and helping to maintain the integrity of skin.

The carob pod extract of the present invention is extracted from wild carob and has galactomannan oligosaccharide as main component. It can provide synergistic regulation for the repairing process of damaged skin caused by various factors and can raise repairing effect.

The yeast β -glucan is a polysaccharide extracted from fungi organisms such as mushrooms, tremella, lucid ganoderma, yeast and the like, has the main active component of β - (1, 3) -D-glucan, has the functions of enhancing immunity and expelling toxin, and has good effects on UV sunburn repair, wound healing, anti-allergy and the like.

The lactobacillus/soybean milk fermentation product filtrate is obtained by fermenting 16 genera and 35 lactobacillus in total from Japanese black soybean milk by an optimized multi-strain fermentation method according to the modern probiotic skin care technology. The skin-nourishing essence contains more than 300 skin-benefiting active substances such as natural moisturizing factors, amino acids, lactic acid, polypeptide, polysaccharide, vitamins, minerals and the like, and has the synergistic anti-inflammatory, anti-allergy and injury repair effects.

The hydrolyzed soybean protein of the invention is prepared by soybeans through a biological enzyme hydrolysis method, is rich in various active substances such as amino acid, soybean isoflavone and the like which are necessary for human bodies, and is an advanced anti-aging plant extract component. Has the effects of moisturizing, resisting oxidation, resisting pollution and the like.

Emulsifying solubilizer

In one embodiment, the emulsification solubilizer is hydrogenated lecithin and/or hydrogenated castor oil.

Preferably, the emulsification solubilizer comprises 0.3-1 part of hydrogenated lecithin and 0.5-1.5 parts of hydrogenated castor oil in parts by weight; more preferably, the emulsification solubilizer is 0.45 part of hydrogenated lecithin and 1 part of hydrogenated castor oil by weight part.

As the hydrogenated castor oil, PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil and PEG-80 hydrogenated castor oil can be exemplified.

Preferably, the hydrogenated castor oil is PEG-40 hydrogenated castor oil.

The applicant has unexpectedly found that when the skin repair agent comprises lactobacillus/soymilk fermentation product filtrate, yeast β -glucan, polypeptide, and polypeptide comprises carnosine, tripeptide-1 copper, oligopeptide-1, palmitoyl pentapeptide-4, the repair effect is better, and unexpectedly found that when the emulsification solubilizer is hydrogenated lecithin and hydrogenated castor oil, the repair effect is further improved, the applicant considers that the possible reason is that yeast- β -glucan and polypeptide are in a spatial helix structure, and the hydrogenated lecithin and hydrogenated castor oil promote the solubilization effect of yeast- β -glucan and polypeptide, and the hydrogenated lecithin and hydrogenated castor oil have better hydrophilic effect, and better affinity with the lactobacillus/soymilk fermentation product filtrate, and the hydrogenated lecithin and hydrogenated castor oil exert the emulsification solubilization effect, simultaneously drive the combination of the lactobacillus/soymilk fermentation product filtrate with sodium and trehalose, and when the molecular weight of sodium hyaluronate is less than 1 dalton, sodium hyaluronate and trehalose are easily absorbed by the skin, thereby promoting the absorption of the skin to the skin repair agent, improving the production of skin collagen and elastin, and improving the appearance of the skin.

< Complex chelating agent >

In one embodiment, the complex chelating agent comprises caprylhydroxamic acid, hexylene glycol, and 1, 3-propanediol.

Preferably, the complex chelating agent comprises 0.005-0.05 part by weight of caprylyl hydroximic acid, 0.03-0.3 part by weight of hexanediol and 0.065-0.65 part by weight of 1, 3-propanediol; more preferably, the complex chelating agent comprises 0.04 parts by weight of caprylyl hydroximic acid, 0.24 parts by weight of hexylene glycol and 0.52 parts by weight of 1, 3-propylene glycol.

In a preferred embodiment, the skin repairing lotion comprises 9.5 parts of moisturizer 10.62 parts of skin repairing agent, 1.45 parts of emulsification solubilizer, 0.8 part of composite chelating agent and deionized water to make up to 100 parts by weight.

The composite chelating agent has good antibacterial effect, is very mild and non-irritant, and has an antiseptic and stabilizing effect on a system.

Carnosine in the invention is a natural endogenous dipeptide, widely exists in muscles, brains and hearts of mammals, and has wide pharmacological activity. Carnosine is proved to have strong free radical scavenging, antioxidant and anti-glycosylation, and effective nursing ingredients capable of effectively protecting skin collagen, dispelling yellow, whitening, resisting aging and maintaining the skin in a young state.

The tripeptide-1 copper in the invention is a complex GHK-Cu formed by a tripeptide (abbreviated as GHK) in human plasma protein and divalent copper ions, and is also commonly called as 'blue copper peptide' because the appearance of the complex is blue, and belongs to one of polypeptides. Modern skin medical research finds that many important metabolic activities (such as barrier immunity, basal cell regeneration, wound healing, toxin discharge and the like) of human skin tissues need copper ions to participate, but the copper ions cannot independently permeate the skin barrier, and skillfully and magically, the GHK can link the copper ions to form the copper-blue peptide and then can be smoothly introduced into the skin to play a role. Therefore, the blue copper peptide has very important functions for repairing and updating skin, and is known as 'repairing peptide' by the medical profession.

The oligopeptide-1 is prepared by an escherichia coli fermentation method and is a water-soluble non-glycosylated small molecular protein peptide. Has the effects of promoting epidermal cell regeneration, accelerating the renewal of aged cells, synergistically resisting aging and the like, and makes the skin smoother, more tender and tender.

The palmitoyl pentapeptide-4 is a modern bioactive polypeptide, is an important signal peptide for regulating the activity of cells and activating certain genes involved in the processes of extracellular matrix renewal and cell proliferation, and has the effects of promoting the generation of type I and III collagen and fibronectin, thereby increasing the thickness of skin and reducing fine wrinkles.

The weight parts of the invention are the same.

In one embodiment, a method of preparing a multi-effect repair spray of a plant protein and polypeptide formulation, comprising the steps of:

(1) adding deionized water into a vacuum homogenizing emulsifying pot at room temperature, heating to 85-90 ℃, keeping for 30 minutes for sterilization, cooling to 60 ℃, sequentially adding sodium hyaluronate, glycerol, 1, 2-pentanediol, hydrogenated lecithin and ceramide 3, stirring for dissolving uniformly, homogenizing for 5-10 min, sequentially adding betaine, trehalose, allantoin, saccharide isomerous bodies and PEG-40 hydrogenated castor oil, stirring uniformly, and homogenizing for 5-10 min;

(2) cooling to 50 deg.C, sequentially adding semen Canavaliae extract, yeast β -dextran, lactobacillus/soybean milk fermentation product filtrate, and compound chelating agent, mixing, stirring, and dissolving;

(3) cooling to below 30 deg.C, adding hydrolyzed vegetable protein, carnosine, tripeptide-1 copper, oligopeptide-1, and palmitoyl pentapeptide-4, and stirring to dissolve.

In a preferred embodiment, a method of preparing a multi-effect repair spray of a plant protein and polypeptide formulation comprising the steps of:

(1) adding deionized water into a vacuum homogenizing emulsifying pot at room temperature, heating to 88 deg.C, maintaining the temperature for 30 min for sterilization, sequentially adding sodium hyaluronate, glycerol, 1, 2-pentanediol, hydrogenated lecithin and ceramide 3 at 60 deg.C, stirring to dissolve uniformly, homogenizing for 4min, sequentially adding betaine, trehalose, allantoin, saccharide isomerate and PEG-40 hydrogenated castor oil, stirring, and homogenizing for 4 min;

The plant protein and polypeptide formula of the invention is filled into an aerosol packaging can by multi-effect repair spraying.

In one embodiment, the aerosol package can comprises a one-component aerosol can and a two-component aerosol can.

Preferably, the multi-effect repair spray of the vegetable protein and polypeptide formulation is bottled into a binary aerosol can.

Aerosol packaging cans are the primary packaging form for "aerosol" products.

The aerosol is a product which hermetically contains the content in a container (generally an aluminum can) with a volume of not more than 1 liter and provided with a valve, and releases the content in a predetermined form under the pressure of a propellant (which may be liquefied petroleum, dimethyl ether, nitrogen, compressed air, or the like) when in use. The product is sprayed, the sprayed material may be in solid, liquid or gaseous state, and the sprayed material may be in the form of mist, foam or powder. The aerosol packaging can is divided into a unitary aerosol can and a binary aerosol can according to whether the propellant is mixed with the content in the can body; unitary aerosol can means that the propellant and contents are mixed together in the can; a binary aerosol can means that the propellant and contents are separated from mixing contact within the can by an internal "bag valve").

Different from the spraying of a traditional hand pump, the positive pressure closed tank of the aerosol product has the advantages of completely isolating air contact and illumination, no need of adding a preservative and the like, can be continuously, conveniently and quickly operated in an integrated spraying mode, and has uniform, soft and comprehensive spraying effect without smearing.

The deionized water in the invention is prepared by the tap water through two-stage Reverse Osmosis (RO) and Electric Desalting (EDI). The product is sterilized at 90 deg.C for 30 min before use.

Examples

Hereinafter, the present invention will be described in more detail by way of examples, but it should be understood that these examples are merely illustrative and not restrictive. The starting materials used in the examples which follow are all commercially available unless otherwise stated.

Example 1

The embodiment 1 of the invention provides a multiple-effect repair spray with a vegetable protein and polypeptide formula, which comprises, by weight, 5.05 parts of a moisturizing agent, 5.93 parts of a skin repair agent, 0.8 part of an emulsification solubilizer, 0.1 part of a composite chelating agent, and 100 parts of deionized water.

The humectant comprises, by weight, 0.05 part of sodium hyaluronate, 0.5 part of trehalose, 2 parts of glycerol, 0.5 part of 1, 2-pentanediol, 1 part of betaine and 1 part of saccharide isomer.

The molecular weight of the sodium hyaluronate is less than or equal to 1 million daltons, and the sodium hyaluronate is purchased from Shandong XifuRuida biological medicine limited company.

The skin repairing agent comprises, by weight, 3 parts of hydrolyzed vegetable protein, 0.23 part of polypeptide, 0.5 part of lactobacillus/soybean milk fermentation product filtrate, 1 part of yeast β -glucan, 0.1 part of ceramide, 0.1 part of allantoin and 1 part of carob bean seed extract, wherein the polypeptide comprises 0.1 part of carnosine, 0.05 part of tripeptide-1 copper, 0.05 part of oligopeptide-1 and 0.03 part of palmitoyl pentapeptide-4.

The emulsifying and solubilizing agent comprises 0.3-1 part of hydrogenated lecithin and 0.5-1.5 part of hydrogenated castor oil in parts by weight.

The complex chelating agent comprises 0.005 parts by weight of caprylyl hydroximic acid, 0.03 parts by weight of hexanediol and 0.065 parts by weight of 1, 3-propanediol.

The hydrolyzed vegetable protein is hydrolyzed soybean protein; the hydrogenated castor oil is PEG-40 hydrogenated castor oil.

The preparation method of the multi-effect repair spray of the plant protein and polypeptide formula comprises the following steps:

(1) adding deionized water into a vacuum homogenizing emulsifying pot at room temperature, heating to 88 ℃, keeping the temperature for 30 minutes for sterilization, cooling to 60 ℃, sequentially adding sodium hyaluronate, glycerol, 1, 2-pentanediol, hydrogenated lecithin and ceramide 3, stirring to dissolve uniformly, homogenizing for 4 minutes, sequentially adding betaine, trehalose, allantoin, saccharide isomerate and PEG-40 hydrogenated castor oil, stirring uniformly, and homogenizing for 4 minutes;

Example 2

The embodiment 2 of the invention provides a multiple-effect repair spray with a formula of vegetable protein and polypeptide, which comprises, by weight, 20 parts of a humectant, 19.05 parts of a skin repair agent, 2.5 parts of an emulsification solubilizer, 1 part of a composite chelating agent, and 100 parts of deionized water.

The humectant comprises 1 part of sodium hyaluronate, 5 parts of trehalose, 5 parts of glycerol, 3 parts of 1, 2-pentanediol, 3 parts of betaine and 3 parts of saccharide isomerate in parts by weight.

The skin repairing agent comprises, by weight, 6 parts of hydrolyzed vegetable protein, 1.55 parts of polypeptide, 1.5 parts of lactobacillus/soybean milk fermentation product filtrate, 3 parts of yeast β -glucan, 1 part of ceramide, 1 part of allantoin and 5 parts of carob bean seed extract, wherein the polypeptide comprises 1 part of carnosine, 0.2 part of tripeptide-1 copper, 0.15 part of oligopeptide-1 and 0.2 part of palmitoyl pentapeptide-4.

The emulsification solubilizer comprises 1 part of hydrogenated lecithin and 1.5 parts of hydrogenated castor oil in parts by weight.

The complex chelating agent comprises 0.05 part of caprylyl hydroximic acid, 0.3 part of hexanediol and 0.65 part of 1, 3-propylene glycol in parts by weight.

The specific implementation mode of the preparation method of the multi-effect repair spray of the plant protein and polypeptide formula is the same as that of example 1.

Example 3

The embodiment 3 of the invention provides a multiple-effect repair spray with a formula of vegetable protein and polypeptide, which comprises 9.5 parts of humectant, 10.62 parts of skin repair agent, 1.45 parts of emulsification solubilizer, 0.8 part of composite chelating agent and 100 parts of deionized water.

The humectant comprises 0.5 part of sodium hyaluronate, 1 part of trehalose, 3 parts of glycerol, 1 part of 1, 2-pentanediol, 2 parts of betaine and 2 parts of saccharide isomerate in parts by weight.

The skin repairing agent comprises, by weight, 4.5 parts of hydrolyzed vegetable protein, 0.52 part of polypeptide, 1.2 parts of lactobacillus/soybean milk fermentation product filtrate, 2 parts of yeast β -glucan, 0.2 part of ceramide, 0.2 part of allantoin and 2 parts of carob bean seed extract, wherein the polypeptide comprises 0.2 part of carnosine, 0.15 part of tripeptide-1 copper, 0.1 part of oligopeptide-1 and 0.07 part of palmitoyl pentapeptide-4.

The emulsification solubilizer comprises 0.45 part of hydrogenated lecithin and 1 part of hydrogenated castor oil in parts by weight.

The complex chelating agent comprises 0.04 parts by weight of caprylyl hydroximic acid, 0.24 parts by weight of hexanediol and 0.52 parts by weight of 1, 3-propanediol.

Example 4

Example 4 of the present invention provides a multi-effect repair spray of a vegetable protein and polypeptide formulation, which is substantially the same as example 3, except that the sodium hyaluronate has a molecular weight of 130 kilodaltons.

Example 5

The embodiment 5 of the invention provides a multi-effect repair spray of a vegetable protein and polypeptide formula, and the specific implementation mode is the same as that of the embodiment 3, except that the trehalose is 0 part by weight.

Example 6

Example 6 of the present invention provides a multi-effect repair spray of a vegetable protein and polypeptide formulation, which is substantially the same as example 3, except that the hydrogenated lecithin is added in an amount of 0 part by weight.

Example 7

The embodiment 7 of the invention provides a multi-effect repair spray of a vegetable protein and polypeptide formula, which has the same specific implementation manner as the embodiment 3, and is different in that the weight part of the lactobacillus/soybean milk fermentation product filtrate is 0.

Example 8

Example 8 of the present invention provides a multi-effect repair spray of a vegetable protein and polypeptide formulation, which is substantially the same as example 3, except that the milk palmitoyl pentapeptide-4 and carnosine are present in an amount of 0 part by weight.

Example 9

The embodiment 9 of the invention provides a multi-effect repair spray of a vegetable protein and polypeptide formula, which is implemented in the same way as the embodiment 3, except that the weight part of the milk palmitoyl pentapeptide-4 is 0.

Example 10

The embodiment 10 of the invention provides a multi-effect repair spray of a vegetable protein and polypeptide formula, and the specific implementation mode is the same as that of the embodiment 3, except that the weight part of carnosine is 0.

Performance evaluation:

1. sensory and physicochemical indexes: the sensory, physicochemical and performance index test results of the multi-effect repair spray obtained in example 3 are shown in table 1.

TABLE 1 sensory, physicochemical indices

2. The hygiene indexes are as follows: the results of the hygiene index tests of the multi-effect repair spray obtained in example 3 are shown in table 2.

TABLE 2 hygiene index

Remarking: the detection method comprises the following steps: cosmetic safety specifications (2015 edition).

3. Toxic substance index: the toxicant indicator test results for the multi-effect repair spray obtained in example 3 are shown in table 3.

TABLE 3 toxic substance index

4. Multiple skin irritation tests: the results of multiple skin irritation tests of the multi-effect repair spray obtained in example 3 are shown in table 4.

Table 4 multiple skin irritation tests

Remarking: the test method comprises the following steps: the test animal is rabbit. Before the test of the test object according to the technical specification of cosmetic safety (2015 edition), the two sides of the spine of the experimental animal are cut off, the unhairing range is 3cmx 3cm respectively, the smearing area is 2.5cmx 2.5cm, the test object is smeared on one side, the other side is used as a control, and the test object is smeared for 1 time every day for 14 days continuously. Starting on day 2, the remaining test substance was removed by shearing before each use, the results were observed after 1 hour, scored according to table 1 in the specification, the mean integral was calculated according to the formula, and the stimulation intensity was graded according to table 2 in the specification.

5. Eye irritation test: the results of the eye irritation test of the multi-effect repair spray obtained in example 3 are shown in table 5.

Table 5 eye irritation test

Remarking: the test method comprises the following steps: the test substance is dripped into the conjunctival sac of eyes at one side of the rabbit, the eyes are closed for one second, and the eyes at the other side are not treated to be used as self control. Observation and rating were required after dropping the test substance according to the cosmetic safety specifications (2015 edition).

6. Actually measuring the human body irritation:

test objects: 50 women of 30-40 years old are selected, the health is required, cosmetics can be used according to the standard, and the testing work is completed by matching with workers according to the requirements.

The using method comprises the following steps: after washing the face with warm clear water, the face is evenly sprayed until the face is completely wet. It is administered 1 time each day in the morning and evening for 1 month.

The evaluation method comprises the following steps: the test subjects evaluated the irritation of the multi-effect healing spray obtained in examples 1 to 3 for four skin reaction states after 1 month of use: a: normal without stimulation; b: slight burning or tingling sensation; c: causing erythema and pruritus; d: severe red swelling and itching pain. The number of people in four skin use states was recorded (table 6).

TABLE 6

From the test results in table 6, the multi-effect repair spray of the plant protein and polypeptide formulation provided by the present invention has a slight stimulation rate (total number of persons with stimulation/total number of persons tested × 100%) of only 4% in 50 subjects, which is substantially consistent with the animal stimulation test results and is safe and non-irritant.

7. And (4) testing the moisturizing effect:

the moisturizing effect after using the test sample (multi-effect repairing spray obtained in example 3) 1 time/day and 5 days is shown in fig. 1.

The test method comprises the following steps: 22 samples of the volunteers who received photoelectric beauty treatment and had dry skin were selected, and the relative value (%) of skin moisture was randomly measured in the marked skin area with a skin moisture tester (SKICON-200EX-USB) after the samples and the control sample (water) were used on the left and right half faces respectively once a day for 5 days.

The results in FIG. 1 show a relative increase of 16% in moisture content with 5 days of use, and a marked difference compared to the control (. times.P.ltoreq.0.01), indicating that it is very effective in moisturizing.

7. Skin barrier damage repair effect test: the test results of the multi-effect repair spray obtained in example 3 are shown in fig. 2.

The test method comprises the following steps: tape tearing model method. Five volunteers were selected, i.e. skin induced erythema (allergic reaction of skin with stimulated increase in blood cell concentration) on the inner side of the arm by tape-tearing, after which the selected areas were treated with the test substance (multi-effect repair spray obtained in example 3) and placebo (water) control, respectively.

In fig. 2, it is shown that the skin TEW L transdermal water loss after tape tearing increases significantly first, and gradually decreases with time as TEW L increases, but after the test substance is used, the decrease increases, and is significantly worse than the blank control and placebo (P ≦ 0.01, TEW L transdermal water tester), indicating that the test substance accelerates the repair of the damaged skin barrier and can provide repair for sensitive skin.

8. Skin improvement effect test:

1) skin elasticity test, the test effect of the multi-effect repair spray obtained in example 3 is shown in figure 3.

The test method comprises the following steps: selecting 12 female healthy volunteers, and applying the test substance (the multi-effect repair spray obtained in example 3) and the control sample (water film) on the left and right half faces respectively once a day; double-blind, random testing; detection indexes are as follows: skin elasticity (skin elasticity tester custom metal MPA 580).

The results in fig. 3 show that the skin elasticity is increased by 12% on average after the multi-effect repairing spray obtained in example 3 is used, and the multi-effect repairing spray has a better anti-aging effect.

2) Facial fine line test, see fig. 4.

The test method comprises the following steps: selecting 12 female healthy volunteers, and using the test substance (the multi-effect repair spray obtained in example 3) and the control sample (water) on the left and right half faces respectively once a day for 25 days; double-blind, random testing; detection indexes are as follows: facial striae (VISA full face shot, striae area calculation in the corner of the eye and cheek regions).

The results in fig. 4 show that the average fine line area after the multi-effect repair spray obtained in example 3 is reduced by 10%, showing that it has a better anti-wrinkle effect.

The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.

Claims

1. The multiple-effect repair spray with the formula of the vegetable protein and the polypeptide is characterized by comprising, by weight, 5-20 parts of a humectant, 5-20 parts of a skin repair agent, 0.5-2.5 parts of an emulsification solubilizer and 100 parts of deionized water, wherein the skin repair agent comprises 3-6 parts of hydrolyzed vegetable protein, 0.2-1.6 parts of polypeptide, 0.5-1.5 parts of lactobacillus/soybean milk fermentation product filtrate and 1-3 parts of yeast β -glucan.

2. The multi-effect repair spray of a plant protein and polypeptide formulation as claimed in claim 1, wherein the skin repair agent further comprises 0.1-1 parts by weight of ceramide, 0.1-1 parts by weight of allantoin, and 1-5 parts by weight of carob seed extract.

3. The multi-effect repair spray of a vegetable protein and polypeptide formulation of claim 1 wherein the emulsification solubilizer is hydrogenated lecithin and/or hydrogenated castor oil.

4. The multi-effect repair spray of a vegetable protein and polypeptide formulation as claimed in claim 3, wherein the emulsification solubilizer comprises 0.3-1 part hydrogenated lecithin and 0.5-1.5 parts hydrogenated castor oil by weight.

5. The multi-effect repair spray of a plant protein and polypeptide formulation as claimed in claim 1, wherein the polypeptide comprises 0.1-1 parts carnosine, 0.05-0.2 parts tripeptide-1 copper, 0.05-0.15 parts oligopeptide-1, 0.03-0.2 parts palmitoyl pentapeptide-4, by weight.

6. The multi-effect repair spray of a plant protein and polypeptide formulation according to any one of claims 1 to 5, wherein the humectant comprises 0.05 to 1 part by weight of sodium hyaluronate and 0.5 to 5 parts by weight of trehalose.

7. The multi-effect repair spray of a plant protein and polypeptide formulation of claim 6, wherein the sodium hyaluronate has a molecular weight of less than or equal to 1 kilodalton.

8. The multi-effect repair spray of a plant protein and polypeptide formulation as claimed in claim 6, wherein the humectant further comprises 2-5 parts by weight of glycerin, 0.5-3 parts by weight of 1, 2-pentanediol, 1-3 parts by weight of betaine, and 1-3 parts by weight of saccharide isomers.

9. The multi-effect repair spray of a plant protein and polypeptide formulation of claim 1, further comprising 0.1 to 1 part by weight of a complex chelating agent; the complex chelating agent comprises caprylyl hydroximic acid, hexanediol and 1, 3-propylene glycol.

10. A method of preparing a multi-effect repair spray of a plant protein and polypeptide formulation according to any one of claims 1 to 9, comprising the steps of: