CN115487114B - Antibacterial peptide composition, acne-removing cream containing antibacterial peptide composition and preparation method of acne-removing cream - Google Patents
Antibacterial peptide composition, acne-removing cream containing antibacterial peptide composition and preparation method of acne-removing cream Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention belongs to the technical field of cosmetics, and particularly relates to an antibacterial peptide composition, acne-removing cream containing the antibacterial peptide composition and a preparation method of the acne-removing cream. The antibacterial peptide composition comprises the following components in parts by weight: 1-5 parts of antibacterial peptide, 1-10 parts of anti-inflammatory agent and 10-50 parts of deionized water; the anti-inflammatory agent is a mixture of Hamamelis mollis extract, olive leaf extract and rose extract. The acne-removing cream containing the antibacterial peptide composition can comprehensively treat and prevent acnes, eliminate skin inflammation, remove acnes rapidly and efficiently, has lasting effect, and also has the effects of promoting skin repair, resisting oxidation, reducing pigmentation and the like.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to an antibacterial peptide composition, acne-removing cream containing the antibacterial peptide composition and a preparation method of the acne-removing cream.
Background
Acne is also called acne, and is a skin disease occurring in the pilo-sebaceous glands, and is frequently found in the abundant sebaceous glands such as the head and face, neck, front chest and back, and is often called acne during puberty, which often affects the work, study and life of patients. It is caused by a variety of factors, including, in addition to genetic and psychological factors: endocrine imbalance, hypersecretion of sebum, hyperkeratosis of the pilosebaceous canal, propionibacterium acnes proliferation and excessive immune response cause perifollicular inflammatory responses. In addition, bad life habits such as frequent stay up, insufficient sleep, excessive eating of spicy, greasy and hot foods, improper use of cosmetics and medicines, etc. may affect, induce and aggravate the occurrence of acne.
At present, a plurality of acne-removing products exist in the market, but the acne-removing effect is often not ideal, and the acne-removing effect is always poor, for example, the acne-removing effect is not thorough, the acne-removing product is easy to relapse, and the damaged skin cannot be completely repaired. The antibacterial peptide disclosed in Chinese patent CN 105411873 is used as an anti-inflammatory and acne-removing agent in the preparation of cosmetics, and is a crude extract or a refined extract of cecropin antibacterial peptide, and contains 37-39 amino acid residues. According to the acne-removing composition containing the antibacterial peptide and the acne-removing cream disclosed by the Chinese patent CN106473944, the antibacterial peptide, the centella asiatica extract and the glycyrrhizin are compounded according to a certain proportion, so that the synergistic effect can be realized, the antibacterial rate of related strains of the acnes is over 90%, the acne-removing composition has remarkable prevention and treatment effects on the acnes, but the repairing effect on the skin is poor, the acne-removing product is too complex in components, and the acne-removing composition has certain irritation to a human body, particularly for a skin sensitive person, the requirements of not only fundamentally removing acnes but also promoting skin repair can not be simultaneously realized.
Therefore, it is highly desirable to provide a product which can not only radically remove acne, but also effectively promote skin repair.
Disclosure of Invention
The invention aims to provide an antibacterial peptide composition, acne-removing cream containing the antibacterial peptide composition and a preparation method of the acne-removing cream. The antibacterial peptide composition can comprehensively treat and prevent acnes, eliminate skin inflammation, quickly and efficiently remove acnes, has lasting effect, and has the effect of promoting skin repair.
In order to achieve the above purpose, the present invention adopts the following technical scheme: an antibacterial peptide composition comprises the following components in parts by weight: 1-5 parts of antibacterial peptide, 1-10 parts of anti-inflammatory agent and 10-50 parts of deionized water;
the anti-inflammatory agent is a mixture of Hamamelis mollis extract, olive leaf extract and rose extract.
Preferably, the antibacterial peptide composition comprises the following components in parts by weight: 2 parts of antibacterial peptide, 6 parts of anti-inflammatory agent and 30 parts of deionized water.
Preferably, the antibacterial peptide is cecropin antibacterial peptide and/or housefly antibacterial peptide.
Preferably, the mass ratio of the witch hazel extract, the olive leaf extract and the rose extract is (3-5): 1.5:1.
the acne-removing cream comprises, by weight, 1-3 parts of an antibacterial peptide composition, 1-5 parts of a stabilizer, 5-20 parts of an emulsifier, 1-15 parts of a humectant, 1-15 parts of an antioxidant, 1-10 parts of a thickener, 2-5 parts of a pH regulator and 1-100 parts of water.
Preferably, the acne-removing cream containing the antibacterial peptide composition at least comprises one of the following (1) to (6):
(1) The stabilizer is at least one selected from liquid paraffin, mineral ester, dioctyl carbonate, triglyceride, palm ethylhexyl ester, dimethyl siloxane, trimethyl siloxane, camellia seed oil, hydrogenated castor oil, squalane, olive fruit oil and rice bran oil;
(2) The emulsifier is at least one selected from glycerol hard fatty acid ester, PEG-100 hard fatty acid, PEG-20 methyl glucose sesquihard fatty acid, tween-20, tween-80, cetostearyl alcohol olive oleate, sorbitan olive oleate and PEG polydimethylsiloxane lecithin;
(3) The humectant is at least one selected from propylene glycol, butanediol, dipropylene glycol, pentanediol, hexanediol, glycerol, octanediol, mannitol, urea, hyaluronic acid, collagen, trehalose, betaine and ceramide;
(4) The antioxidant is at least one selected from vitamin A, vitamin C, vitamin E, sodium sulfite, sodium thiosulfate, carnosine, glutathione, cysteine and histidine;
(5) The thickener is at least one selected from yellow collagen, polyethylene glycol, carbomer 940, hydroxyethyl cellulose and acrylic ester;
(6) The pH regulator is fruit acid and/or sodium hydroxide.
The preparation method of the acne-removing cream containing the antibacterial peptide composition comprises the following steps of:
sequentially adding an antioxidant, a stabilizer, an emulsifier, a thickener, a humectant and water into the antibacterial peptide composition, homogenizing and stirring until the antibacterial peptide composition is completely dissolved, then adding water and a pH regulator, regulating the pH value of the solution, uniformly stirring, cooling, and filtering to obtain the acne removing cream containing the antibacterial peptide composition.
Preferably, the speed of the homogenizing stirring is 2000-3000 rpm/min, and the homogenizing stirring temperature is 25-100 ℃; the pH value of the solution is adjusted to 4.0-8.5.
Use of the antimicrobial peptide composition in the preparation of cosmetics. The cosmetic is in the form of cream, emulsion, essence, lyophilized powder, gel or facial mask.
The antibacterial peptide has broad-spectrum antibacterial property, is rapid in sterilization, does not generate resistance and drug resistance, has huge application potential in the aspects of anti-infection and anti-inflammatory treatment for treating skin diseases such as acne, allergic dermatitis and the like, can effectively kill or inhibit propionibacterium acnes, but cannot solve the serious acne problem when being singly used.
The witch hazel extract is rich in kaempferol, quercetin and astragaloside, contains various tannin components, has remarkable effects of astringing, controlling oil and sterilizing, is suitable for skin with serious adolescence or oil production, can remarkably reduce the level of inflammatory factor interleukin-6 (IL-6) in the cell inflammation process, and can promote the synthesis of type I collagen, thereby achieving the effects of eliminating skin inflammation and repairing skin.
The olive leaf extract can stimulate macrophage activity, promote inflammatory reaction at early stage of inflammation, kill bacteria and phagocytose, inhibit release of histamine, reduce mRNA expression of inflammatory factors TNF-alpha and IL-1β, and has antiinflammatory effect, and inhibit inflammatory reaction at late stage of inflammation, promote cell proliferation and collagen deposition, improve red blood streak caused by inflammation, stimulate desquamation, inflammatory vaccinia, and post-inflammatory pigmentation.
The flos Rosae Rugosae extract has effects of enhancing cell activity, activating skin, and resisting inflammation and keeping moisture.
The components in the invention are compounded and used to inhibit cell degeneration, enhance the immune function of cells, repair damaged cells, accelerate the metabolism of cells and reduce the skin oxidation speed, thereby improving skin relaxation, reducing wrinkles, activating the activity of cells, effectively helping human bodies to remove harmful free radicals, reducing pigmentation and improving skin darkness, simultaneously, the antibacterial peptide and the anti-inflammatory agent jointly act on the surface of the skin, can modulate the immune defense system of the skin, act as chemokines of monocytes and T lymphocytes, enable the chemokines to be rapidly gathered at the position of inflammatory reaction, promote the expression of organic chemical factors and the proliferation reaction of T-assisted lymphocytes, inhibit the cytokines harmful to hosts caused by germ substances, promote the apoptosis of macrophages and activated lymphocytes, and further improve the acne removing and skin repairing effects.
Compared with the prior art, the invention has the following beneficial effects:
(1) The antibacterial peptide and the anti-inflammatory agent are compounded for use, so that the anti-acne effect of the active substance is enhanced, the skin is promoted to repair itself, the acne can be removed fundamentally, the damaged skin can be repaired, the pigmentation is reduced, and the like, and the antibacterial peptide and the anti-inflammatory agent are non-irritating and suitable for people with sensitive skin.
(2) The antibacterial peptide composition is applied to the field of cosmetics, the high-efficiency antibacterial effect of the antibacterial peptide composition on bacteria, saccharomycetes and moulds is fully utilized, and the use of traditional chemical preservatives can be reduced.
Drawings
Fig. 1 is a graph showing the acne-removing effect of example 4 of the present invention on a subject.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the examples and comparative examples, the experimental methods used were conventional methods, and the materials, reagents and the like used were commercially available, unless otherwise specified.
For example, the antibacterial peptide is selected from cosmetic grade fermentation synthetic antibacterial peptide of North China pharmacy, CAS 80451-04-3, and the activity is 5000IU/ml;
the Hamamelis mollis extract is selected from Shanxi Yuning Biotechnology Co., ltd, CAS 469-32-9;
the olive leaf extract is selected from products of Shaanxi Ruiwo biotechnology Co., ltd, CAS 32619-42-4;
the rose extract is selected from Shanghai leaf biotechnology limited, CAS 532-82-1;
example 1 antimicrobial peptide compositions of the invention
The antibacterial peptide composition disclosed by the invention comprises the following components in parts by weight:
2.0 parts of cecropin antibacterial peptide, 6.0 parts of anti-inflammatory agent and 30.0 parts of deionized water.
Wherein the anti-inflammatory agent is a composition of witch hazel extract, olive leaf extract and rose extract, and the mass ratio of the witch hazel extract, the olive leaf extract and the rose extract is 4:1.5:1.
example 2 antimicrobial peptide compositions of the invention
The antibacterial peptide composition disclosed by the invention comprises the following components in parts by weight:
1.0 part of housefly antibacterial peptide, 1.0 part of anti-inflammatory agent and 10.0 parts of deionized water.
Wherein the anti-inflammatory agent is a composition of witch hazel extract, olive leaf extract and rose extract, and the mass ratio of the witch hazel extract, the olive leaf extract and the rose extract is 3:1.5:1.
example 3 antimicrobial peptide compositions of the invention
The antibacterial peptide composition disclosed by the invention comprises the following components in parts by weight:
5.0 parts of cecropin antibacterial peptide, 10.0 parts of anti-inflammatory agent and 50.0 parts of deionized water
Wherein the anti-inflammatory agent is a composition of witch hazel extract, olive leaf extract and rose extract, and the mass ratio of the witch hazel extract, the olive leaf extract and the rose extract is 5:1.5:1.
example 4 acne-removing cream containing the antibacterial peptide composition of the present invention and preparation method thereof
The acne-removing cream containing the antibacterial peptide composition comprises the following components in parts by weight:
the preparation method comprises the following steps: sequentially adding an antioxidant, a stabilizer, an emulsifier, a thickener, a humectant and water into the antibacterial peptide composition, homogenizing and stirring at 2500rpm/min and 45 ℃ until the antibacterial peptide composition is completely dissolved, then adding water and a pH regulator, regulating the pH value of the solution to 7.0, uniformly stirring, cooling to room temperature, and filtering to obtain the acne removing cream containing the antibacterial peptide composition.
Examples 5 to 6, acne-removing cream containing the antibacterial peptide composition of the present invention and preparation method thereof
The antibacterial peptide compositions of examples 2 to 3 were used in place of the antibacterial peptide composition of example 1 to prepare anti-acne creams containing the antibacterial peptide compositions of examples 5 to 6, respectively.
Comparative example 1
The present comparative example is different from example 4 in that cecropin was removed from the cecropin composition of example 1, and the remaining components and preparation method are described in reference to example 4.
Comparative example 2
The difference of this comparative example compared to example 4 is that the anti-inflammatory agent in the antimicrobial peptide composition of example 1 is replaced with palmitoyl tetrapeptide-7, and the remaining components and preparation methods are referred to in example 4.
Comparative example 3
The difference of this comparative example compared to example 4 is that the antimicrobial peptide composition of example 1 lacks witch hazel extract in the anti-inflammatory agent, and the remaining components and preparation method are referred to in example 4.
Comparative example 4
The difference of this comparative example compared to example 4 is that the anti-inflammatory agent of the antibacterial peptide composition of example 1 lacks olive leaf extract, and the rest of components and preparation method refer to example 4.
Comparative example 5
Compared with example 4, the present comparative example is different in that the mass ratio of witch hazel extract, olive leaf extract and rose extract in the antibacterial peptide composition anti-inflammatory agent of example 1 is 1:1.5:1.
comparative example 6, existing polypeptide composition for acne removal and skin repair and method for preparing the same
The formula comprises the following components: 5 parts of antibacterial peptide, 3 parts of anti-inflammatory factor, 2 parts of cell growth factor, 60 parts of stabilizer, 30 parts of humectant, 15 parts of antioxidant, 30 parts of solubilizer and 950 parts of water.
The antibacterial peptide comprises 3 parts of myristoyl hexapeptide-23 and 2 parts of polylysine.
The anti-inflammatory factor is palmitoyl tetrapeptide-7.
The cell growth factor comprises 1 part of oligopeptide-1 and 1 part of oligopeptide-5.
The stabilizer is 30 parts of glycerol and 30 parts of propylene glycol.
The humectant is aloe vera gel.
The antioxidant is 7 parts of sodium sulfite and 8 parts of vitamin E.
The solubilizer is PEG-400.
The preparation method comprises the following steps:
(1) Weighing the components according to the formula amount, adding 600 parts of water into a batching tank, then sequentially adding a solubilizer, antibacterial peptide, anti-inflammatory factors and cell growth factors, and fully stirring until the components are completely dissolved to obtain a mixture A;
(2) Then dissolving an antioxidant by using 100 parts of water, and adding the antioxidant into a material mixing tank to be mixed with the mixture A to obtain a mixture B;
(3) Then dissolving the stabilizer and the humectant with 100 parts of water, adding the mixture into a batching tank, mixing with the mixture B, and uniformly stirring to obtain a mixture C;
(4) And (3) dropwise adding citric acid into the mixture C, stirring the mixture C while dropwise adding, regulating the pH value of the mixture C to 3.0, adding the rest of water into the mixture C, stirring uniformly, and filtering the mixture C by using a 0.22 mu m PES (polyether sulfone) filter membrane to prepare the polypeptide composition for removing acnes and repairing skin.
Reference CN 109498501A example 4.
Test example one, safety test
1. Human skin patch test
Through a human skin patch test, the potential possibility of adverse reaction of human skin caused by the acne-removing functional skin care product is detected. Samples of examples 4 to 6 were tested according to the requirements of the human skin patch test method of cosmetic safety Specification (2015 edition). The results show that compared with the skin response grading standard of the skin closed patch test, the skin response is a negative response-suspicious response, only weak erythema is generated, and the corresponding grading is 0-1.
The experiment shows that the samples of the embodiments 4 to 6 of the invention have no adverse reaction to human skin.
2. Dermatitis test: repeated exposure to the product of the invention and its ingredients was determined by the guinea pig allergic contact dermatitis test to determine whether and to what extent the mammal could be allergic. Samples of examples 4 to 6 and comparative examples 1 to 6 were tested according to the requirements of the skin allergy test method of cosmetic safety Specification (2015 edition). The scoring criteria are shown in the following table:
TABLE 1 skin allergy scoring criteria
Table 2 skin allergy scoring results for each sample
As can be seen from the data in Table 2, the acne cream containing the antibacterial peptide composition prepared by the embodiment of the invention has lower skin irritation and sensitization, and the compound use of the antibacterial peptide and the anti-inflammatory agent can reduce the irritation and sensitization of the prepared product to the skin.
Test example two, in vitro antibacterial test on Propionibacterium acnes
1. Experimental samples: acne-removing cream and blank control group (physiological saline) containing antibacterial peptide compositions prepared in examples 4 to 6 and comparative examples 1 to 6
2. The experimental method comprises the following steps: the same dose of the sample was taken, added to quantitative MH agar thawed and cooled to about 50℃to prepare plates containing different sample concentrations, and then propionibacterium acnes were inoculated, cultured under environmental conditions suitable for the growth of the bacteria, the growth of propionibacterium acnes in the culture dish was observed, and the number of colonies in the plates was counted, and experimental data are shown in Table 3.
Table 3 data of the antibacterial experiments of samples against Propionibacterium acnes
As can be seen from the data in Table 3, the acne-removing creams prepared in examples 4 to 6 of the invention can effectively inhibit the growth of Propionibacterium acnes, which shows that the antibacterial peptide and the anti-inflammatory agent can play a synergistic role in combination.
Comparative example 1 compared to the inventive example, the final bacteriostatic effect was significantly worse than in the example due to the lack of one component of the antimicrobial peptide composition, the replacement of the anti-inflammatory component with an equivalent amount of palmitoyl tetrapeptide-7 in comparative example 2; the comparative examples 3 to 4 lack the witch hazel extract and olive leaf extract components, respectively, cannot produce synergistic effect with other components, and finally have poor antibacterial effect; comparative example 5 the weight ratio of witch hazel extract, olive leaf extract and rose extract in the anti-inflammatory component is not suitable, resulting in poor antibacterial effect of the finished product; comparative example 6 is a prior polypeptide composition for removing acne and repairing skin, which has a bacteriostatic effect inferior to that of the present example.
Test example III, skin repair Effect test
1. Experimental sample
The anti-acne cream containing the antibacterial peptide composition prepared in examples 4 to 6 and comparative examples 1 to 6 was tested for skin moisture, elasticity, antioxidation and whitening effects.
2. Experimental method
2.1 skin moisture content (MMV) test
45 volunteers are selected and randomly divided into 9 groups, 5 persons in each group mark the inner sides of the left arm and the right arm of the volunteers with test areas of 3cm multiplied by 3cm, and the same arm can mark a plurality of areas at intervals of 1cm. Test samples were randomly distributed on the left and right arms. Test area and pair using capacitive skin assayAnd (5) measuring the illumination area. The blank value of each test area was measured first and then at a rate of 2.0mg/cm 2 The test product was uniformly coated in the test area using latex fingerstall. After 2h of application, the skin moisture content of the test and control areas was measured and the average value was recorded. The MMV value calculation method is as follows:
skin moisture content increase rate (%) = (post-application moisture measurement-pre-application moisture measurement)/pre-application moisture measurement x 100%
2.2 skin elasticity test
Selecting 100 volunteers, randomly dividing into 10 groups of 10 persons, wherein 1 group is blank group, using water as test sample, sequentially and circularly marking left and right cheeks of a subject, randomly distributing test and control substances on the left and right cheeks, smearing corresponding samples on a test area and a control area for 2 times in the morning and evening every day, and respectively smearing 2.0mg/cm every time 2 After 4 weeks of continuous use, the measured parts were measured using a skin elasticity tester Revisclometer RV600 and the average value was recorded.
The skin elasticity value is expressed by the ratio of the rebound quantity of the skin without negative pressure to the maximum stretching quantity with negative pressure, and the closer the ratio is to 1, the better the skin elasticity is. The test adopts the elasticity parameter R value as an evaluation basis, is used for evaluating the change and influence of a sample on skin elasticity, and calculates the elasticity change rate by the following method:
elastic change rate = R value after sample application/R value before sample application
2.3 antioxidant test
The free activity of sample clearance was determined using the 1,1-diphenyl-2-picrydrazyl (DPPH) method. The samples of examples 4 to 6 and comparative examples 1 to 6 were prepared as solutions having a concentration of 1mg/ml using purified water as a diluent, 1.0ml was taken, 3.0ml of DPPH solution (0.004% concentration) was added thereto, and the reaction was carried out at room temperature for 30 minutes in the absence of light, while the absorbance was measured at a wavelength of 517nm using absolute ethyl alcohol as a blank. The DPPH radical scavenging rate was calculated as follows:
clearance (%) = (absorbance after 1-sample DPPH reaction/blank DPPH absorbance value) ×100%
2.4 whitening test
The test adopts a test method for melanin inhibition of the melanoma cells of the mice, and is used for evaluating the whitening efficacy of the sample.
Preparing a sample solution: the samples of examples 4 to 6 and comparative examples 1 to 6 were prepared as solutions having a volume concentration of 1% using a serum-containing complete culture solution as a diluent, and were sterilized by filtration through a 0.22 μm filter.
Taking melanoma cells of B16 mice in logarithmic growth phase at a ratio of 2×10 4 The cells were inoculated at a density of 100. Mu.L/well into 96-well cell culture plates, and incubated overnight in a carbon dioxide incubator. The old culture solution is discarded, a sample with the volume concentration of 1% is added into the experimental group, the same amount of serum-containing cell culture solution is added into the normal control group, and after the culture is continued for 24 hours, the relative proliferation rate (RGR) of cells is detected by adopting a WST-1 method.
Taking B16 cells in logarithmic growth phase at 2×10 4 Inoculating 3mL of each of the culture plates with the density of 3mL in 6 holes, placing the culture plates in a carbon dioxide incubator for conventional culture for 24 hours, discarding old culture solution, adding 3mL of serum-containing culture solution into a normal control group and a model group, adding 3mL of sample solution into the model group, adding 60 mu L of forskolin solution (2 mM) into each hole of the model group and the model group, stimulating melanin production, and continuously culturing for 72 hours.
Measurement of intracellular melanin content: pancreatin digestion, centrifugation to remove supernatant, adding 1mol/L NaOH solution 500 μL to lyse cells, heating at 80deg.C for 30min, centrifuging at 3000rpm for 10min, adding 96-well plate into the supernatant, 100 μL/well, measuring 450nm absorbance to obtain total melanin, taking the melanin content of model group as 100%, obtaining the relative value and inhibition rate of melanin content of experimental group, and calculating total melanin content inhibition rate by the following method:
inhibition (%) = (1-experimental group absorbance value/model group absorbance value) ×100%
3. Experimental results
3.1 skin moisture content (MMV) test results
TABLE 4 skin moisture content (MMV) test results
As can be seen from the data in Table 4, the moisture content increase rate of the samples of examples 4 to 6 according to the present invention was significantly higher than that of the samples of comparative examples 1 to 6 after each sample was used for 2 hours, indicating that the moisture retention effect of the samples of examples was good.
3.2 results of skin elasticity test
TABLE 5 skin elasticity test results
| Group of | Skin elasticity parameter R value | Rate of elastic change |
| Blank group | 0.51 | 1.00 |
| Example 4 | 0.85 | 1.66 |
| Example 5 | 0.75 | 1.47 |
| Example 6 | 0.79 | 1.55 |
| Comparative example 1 | 0.63 | 1.27 |
| Comparative example 2 | 0.65 | 1.32 |
| Comparative example 3 | 0.67 | 1.35 |
| Comparative example 4 | 0.69 | 1.37 |
| Comparative example 5 | 0.71 | 1.40 |
| Comparative example 6 | 0.70 | 1.38 |
As is clear from the data in Table 5, the values of the elastic parameters R of the samples of examples 4 to 6 and comparative examples 1 to 6 were all increased when the samples were used for four weeks, that is, the ratio of the rebound amount of the skin without negative pressure to the maximum stretching amount with negative pressure was increased, indicating that the elastic properties of the skin were recovered in each of examples 4 to 6 and comparative examples 1 to 6. According to the elastic change rate value, the elastic change rate of the samples of the examples 4 to 6 is higher than that of the comparative examples, which shows that the anti-aging effect of the samples of the examples 4 to 6 is better than that of the comparative examples 1 to 6, wherein the elastic change rate of the sample of the example 4 is the highest, and the best effect is shown.
3.3 antioxidant test results
TABLE 6 DPPH radical scavenging Activity test results
As can be seen from the data in Table 6, the DPPH free radical scavenging ability of the invention in examples 4 to 6 is stronger than that in comparative examples 1 to 6 at the same concentration, which shows that the antibacterial peptide and the anti-inflammatory agent can also play a synergistic role in terms of antioxidation when being compounded for use.
3.4 whitening test results
TABLE 7 melanin total content inhibition test results
From the data in Table 7, it is understood that examples 4 to 6 and comparative examples 1 to 6 exhibited some inhibition of proliferation of B16 cells and secretion of melanin, and that the whitening effect of example 4 was more remarkable.
The test results of the four tests are combined, and the samples of the examples 4-6 are obviously better than the samples of the comparative examples 1-6 in terms of moisturizing effect and improvement on skin elasticity, namely, the acne cream containing the antibacterial peptide composition has obvious effect on skin restoration.
Test example four, acne-removing Effect test
5 volunteers with different degrees of acne on the face are selected, and the ages are 12-45 years. The acne cream containing the antibacterial peptide composition prepared in the embodiment 4 of the invention is smeared on the face according to the use method, and the acne effect and the desalination of acne pits and acne marks are observed after 5mL each time and 14 days.
The results are shown in figure 1, and the product has remarkable effect on inflammation caused by acne and has the effect of removing acne pits and acne marks. After volunteers participate in the test, after the product is continuously used for half a month, wrinkles are reduced, skin color is improved, pores are contracted, melanin pigmentation is reduced, and the effect of promoting skin repair is achieved.
The above embodiments are merely illustrative of the principles of the present invention and its effectiveness, and are not intended to limit the invention. Modifications and variations may be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the invention. Accordingly, it is intended that all equivalent modifications and variations of the invention be covered by the claims, which are within the ordinary skill of the art, be within the spirit and scope of the present disclosure.
Claims (7)
1. The antibacterial peptide composition is characterized by comprising the following components in parts by weight: 1-5 parts of antibacterial peptide, 1-10 parts of anti-inflammatory agent and 10-50 parts of deionized water;
the anti-inflammatory agent is a mixture of witch hazel extract, olive leaf extract and rose extract;
the mass ratio of the witch hazel extract to the olive leaf extract to the rose extract is (3-5): 1.5:1, a step of;
the antibacterial peptide is cecropin antibacterial peptide and/or housefly antibacterial peptide;
the CAS of the cecropin antibacterial peptide is 80451-04-3;
the CAS of the Hamamelis mollis extract is 469-32-9;
the CAS of the olive leaf extract is 32619-42-4.
2. The antibacterial peptide composition as claimed in claim 1, which comprises the following components in parts by weight: 2 parts of antibacterial peptide, 6 parts of anti-inflammatory agent and 30 parts of deionized water.
3. An anti-acne cream containing an antibacterial peptide composition is characterized by comprising, by weight, 1-3 parts of the antibacterial peptide composition according to any one of claims 1-2, 1-5 parts of a stabilizer, 5-20 parts of an emulsifier, 1-15 parts of a humectant, 1-15 parts of an antioxidant, 1-10 parts of a thickener, 2-5 parts of a pH regulator and 1-100 parts of water.
4. The acne-removing cream of claim 3, wherein the acne-removing cream comprises at least one of the following (1) - (6):
(1) The stabilizer is at least one selected from liquid paraffin, mineral ester, dioctyl carbonate, triglyceride, palm ethylhexyl ester, dimethyl siloxane, trimethyl siloxane, camellia seed oil, hydrogenated castor oil, squalane, olive fruit oil and rice bran oil;
(2) The emulsifier is at least one selected from glycerin hard fatty acid ester, PEG-100 hard fatty acid, PEG-20 methyl glucose sesquihard fatty acid, tween-20, tween-80, cetostearyl alcohol olive oleate, sorbitan olive oleate, PEG polydimethylsiloxane and lecithin;
(3) The humectant is at least one selected from propylene glycol, butanediol, dipropylene glycol, pentanediol, hexanediol, glycerol, octanediol, mannitol, urea, hyaluronic acid, collagen, trehalose, betaine and ceramide;
(4) The antioxidant is at least one selected from vitamin A, vitamin C, vitamin E, sodium sulfite, sodium thiosulfate, carnosine, glutathione, cysteine and histidine;
(5) The thickener is at least one selected from yellow collagen, polyethylene glycol, carbomer 940, hydroxyethyl cellulose and acrylic ester;
(6) The pH regulator is fruit acid and/or sodium hydroxide.
5. A method for preparing the anti-acne cream containing the antibacterial peptide composition according to claim 3 or 4, comprising the following steps:
sequentially adding an antioxidant, a stabilizer, an emulsifier, a thickener, a humectant and water into the antibacterial peptide composition, homogenizing and stirring until the antibacterial peptide composition is completely dissolved, then adding water and a pH regulator, regulating the pH value of the solution, uniformly stirring, cooling, and filtering to obtain the acne removing cream containing the antibacterial peptide composition.
6. The preparation method according to claim 5, wherein the speed of the homogenizing stirring is 2000-3000 rpm, and the homogenizing stirring temperature is 25-100 ℃; and the pH value of the solution is regulated to 4.0-8.5.
7. The use of the antibacterial peptide composition according to any one of claims 1 to 2 in the preparation of cosmetics.
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