CN114886813B - Anti-aging plant extract composition and preparation method and application thereof - Google Patents
Anti-aging plant extract composition and preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of cosmetics, and discloses an anti-aging plant extract composition, and a preparation method and application thereof. The anti-aging plant extract composition comprises extracts of magnolia liliiflora, rhizoma gastrodiae, dendrobium nobile stems and millet seeds. The plant extract composition provided by the invention effectively exerts a synergistic effect through different action mechanisms of all raw materials, has the effects of tightening and resisting wrinkles, effectively improves skin aging phenomena such as skin relaxation, wrinkles, dryness, water shortage, dark skin color and the like, relieves skin aging, and enables the skin to recover a young state.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to an anti-aging plant extract composition, and a preparation method and application thereof.
Background
According to the cause of aging, skin aging can be classified mainly into intrinsic aging and extrinsic aging. Endogenous aging is caused by the body's own factors, such as heredity, disease, endocrine disorders, sleep deficits, and the like. Exogenous aging is mainly the result of the superposition of photodamaged free radical accumulation and natural aging, wherein photoaging mainly occurs on the skin of the light-exposed parts such as the face, the neck, the forearm and the like. Aged skin, reduced collagen and elastin content, leading to the collapse of the skin's intrinsic structure, leading to the appearance of wrinkles; the barrier function of the horny layer is damaged, hyaluronic acid is easy to lose, and the water content of the skin is reduced, so that the skin is rough, dry and lack of water; pigmentation is caused by photoaging, increased free radicals in the body, and damage to cellular structures and DNA. Skin aging is mainly manifested by: loose skin, wrinkles, dry and thin skin, pigmentation, etc.
The traditional anti-aging skin care product simply supplements the collagen content of the skin, and a single anti-aging path often has poor effect and is easy to repeat, so that the anti-aging effect cannot reach the expectation.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art described above. Therefore, the anti-aging plant extract composition provided by the invention has the effects of relatively strong water replenishing and moisturizing, free radical scavenging, skin collagen production promoting, expression line improving, wrinkle fading, wrinkle tightening, wrinkle resisting and the like, starts with various approaches for delaying skin aging, and comprehensively repairs aged skin, so that the aging phenomena of skin wrinkles, looseness, dryness, water shortage, darkness and the like are really solved.
In a first aspect of the invention, a plant extract composition is provided.
Specifically, the plant extract composition comprises extracts of various raw materials of magnolia liliiflora, rhizoma gastrodiae, dendrobium stem and millet seeds.
The plant extract composition has the following effects:
magnolia liliiflora, also known as Magnolia liliiflora, is a magnolia plant, and contains a large amount of lignin and new lignin components in the flower buds. Lignans and neolignans can improve SOD activity, and have effects of scavenging free radicals and reducing MDA (malondialdehyde) level to achieve antioxidant effect; and can resist acetylcholine release and contract muscle fiber, thereby preventing expression lines and wrinkles. The purple jade orchid bud also contains a certain amount of volatile oil components, and has strong antibacterial, anti-inflammatory, soothing and anti-allergy effects.
Gastrodia elata is dried tuber of Orchidaceae plant, and is a rare medicinal material for food and medicine. According to the records of the literature, the gastrodia elata has the effects of improving intelligence, strengthening brain and delaying aging. The rhizoma gastrodiae extract contains rich gastrodin, and the gastrodin can improve the activity of SOD and reduce the MDA level, has strong effects of resisting oxidation and removing free radicals, and can play the effects of activating skin, improving skin luster and homogenizing skin color.
Dendrobium nobile is a traditional famous and precious traditional Chinese medicine in China, and is listed as the top grade in the Shen nong's herbal Jing. Modern medicine and traditional Chinese medicine pharmacological research show that the dendrobium nobile has obvious effects on improving the human body immunity, resisting aging, inhibiting tumor, tonifying internal organs, consumptive disease and the like. The dendrobium stem extract contains rich dendrobium polysaccharide, and has the effects of promoting the expression of skin aquaporin APQ-3 and keeping moisture strongly.
Millet seeds, belonging to one of five cereals, are rich in amino acids, folic acid, vitamins, trace elements and metasilicic acid, which is an essential component of skin connective tissue and has the effects of promoting collagen synthesis and stabilizing the network structure of collagen and elastin in the dermis, thereby tightening the skin and resisting wrinkles.
The plant extraction composition adopts extracts of various raw materials of magnolia liliiflora, gastrodia elata roots, dendrobium stem and millet seeds, wherein the magnolia liliiflora extract has the effects of resisting expression lines and tightening pale lines, the gastrodia elata roots extract has the effects of scavenging free radicals and resisting oxidation, the dendrobium nobile stem extract has the effects of efficiently replenishing water and preserving moisture, and the millet seed extract promotes collagen synthesis and stabilizes the collagen network structure of skin to achieve the tightening effect; the invention achieves the aim of resisting skin aging by a plurality of ways of moisturizing, scavenging free radicals, promoting collagen generation, relaxing veins and resisting wrinkles, and the like, wherein the components are synergistic and complement each other.
Preferably, the mass parts of the raw materials are as follows:
5-35 parts of magnolia liliiflora, 5-35 parts of rhizoma gastrodiae, 5-30 parts of dendrobium stem and 5-30 parts of millet seeds.
Preferably, the mass parts of the raw materials are as follows:
5-30 parts of magnolia liliiflora, 5-30 parts of rhizoma gastrodiae, 5-25 parts of dendrobium stem and 5-25 parts of millet seeds.
The second aspect of the present invention provides a method for preparing the plant extract composition.
Specifically, the preparation method of the plant extract composition comprises the following steps:
mixing the raw materials, and extracting to obtain the plant extract composition.
Preferably, the preparation method of the plant extract composition comprises the following steps:
pulverizing Magnolia liliflora, radix Gastrodiae, herba Dendrobii, and semen Panici Miliacei, mixing, adding into solvent, heating for extraction, filtering, and collecting extractive solution to obtain the plant extract composition.
Preferably, the preparation method of the plant extract composition comprises the following steps:
pulverizing Magnolia liliflora, radix Gastrodiae, herba Dendrobii, and semen Panici Miliacei into 100-200 mesh powder, mixing, adding into solvent at a ratio of material to liquid of 1 (5-30), extracting at 50-80 deg.C for 0.5-3 hr for 1-3 times, filtering, and collecting extractive solution to obtain the plant extract composition.
Preferably, the preparation method of the plant extract composition comprises the following steps:
pulverizing Magnolia liliflora with a pulverizer to 100-200 meshes, adding into solvent according to the material-liquid ratio of 1 (5-30), heating and extracting at 50-80 deg.C for 0.5-3h for 1-3 times, filtering, and collecting the extractive solution to obtain Magnolia liliflora extract;
pulverizing rhizoma Gastrodiae into 100-200 mesh powder with pulverizer, adding into solvent according to material-to-liquid ratio of 1 (5-30), extracting at 50-80 deg.C for 0.5-3 hr, extracting for 1-3 times, filtering, and collecting extractive solution to obtain rhizoma Gastrodiae extract;
pulverizing herba Dendrobii with pulverizer to 100-200 mesh, adding into solvent according to material-to-liquid ratio of 1 (5-30), heating and extracting at 50-80 deg.C for 0.5-3 hr, extracting for 1-3 times, filtering, and collecting extractive solution to obtain the extract;
pulverizing semen Panici Miliacei to 100-200 mesh with pulverizer, adding into solvent according to material-to-liquid ratio of 1 (5-30), extracting at 50-80 deg.C for 0.5-3 hr for 1-3 times, filtering, and collecting extractive solution to obtain semen Panici Miliacei extract;
mixing the magnolia liliiflora extract, the gastrodia elata root extract, the dendrobium nobile extract and the millet seed extract to obtain the plant extract composition.
Preferably, in the preparation method of the plant extract composition, the solvent is water or an ethanol solution with a mass concentration of 75-95%.
In order to make the plant extract composition of the present invention less susceptible to deterioration, a proper amount of preservative may be added to the plant extract composition.
The third aspect of the present invention provides the use of the plant extract composition for the preparation of cosmetics.
Preferably, the cosmetic comprises the plant extract composition and auxiliary materials.
Preferably, the plant extract composition accounts for 0.01-20% of the cosmetic by mass.
Preferably, the plant extract composition accounts for 0.01-2% of the weight of the cosmetic.
Preferably, the auxiliary materials comprise at least one of an emulsifier, a thickener, grease, an antioxidant and a preservative.
Preferably, the emulsifier comprises Montanov 68 (cetearyl alcohol, cetearyl glucoside), montanov L (C14-22 alcohol, C12-20 alkyl glucoside),
1000 (cetearyl olive oleate, sorbitan olive oleate), SP MYRJ S40 (PEG-40 stearate).
Preferably, the oil comprises at least one of squalane, jojoba oil, caprylic/capric triglyceride, shea butter, and polydimethylsiloxane.
Preferably, the antioxidant comprises at least one of p-hydroxyacetophenone, tocopherol (vitamin E), tocopherol acetate.
Preferably, the thickener comprises at least one of xanthan gum, hydroxyethyl cellulose, ammonium acryloyldimethyl taurate/VP copolymer, carbomer.
Preferably, the preservative comprises at least one of phenoxyethanol, methylparaben, propylparaben, and sodium benzoate.
Compared with the prior art, the invention has the following beneficial effects:
the plant extract composition provided by the invention adopts extracts of various raw materials of magnolia liliiflora, rhizoma gastrodiae, dendrobium stem and millet seeds, effectively exerts a synergistic effect through different action mechanisms of the raw materials, has the effects of tightening and resisting wrinkles, effectively improves skin aging phenomena such as skin relaxation, wrinkles, dry and water shortage, dark skin color and the like, relieves skin aging, and enables the skin to recover a young state.
Drawings
FIG. 1 is a graph showing the change in skin elasticity index of a subject in Experimental example 5 of the present invention.
Detailed Description
In order to make the technical solutions of the present invention more apparent to those skilled in the art, the following examples are given for illustration. It should be noted that the following examples are not intended to limit the scope of the claimed invention.
The starting materials, reagents or apparatuses used in the following examples are conventionally commercially available or can be obtained by conventionally known methods, unless otherwise specified.
Example 1
An anti-aging plant extract composition comprises the following extracts of raw materials in parts by mass: 30 parts of magnolia liliiflora, 30 parts of rhizoma gastrodiae root, 20 parts of dendrobium stem and 20 parts of millet seed.
The preparation method of the anti-aging plant extract composition comprises the following steps: weighing the raw materials of magnolia liliiflora, rhizoma gastrodiae, dendrobium nobile and millet seeds according to the proportion, crushing the raw materials into 150 meshes by a crusher, mixing, adding the mixture into 75% ethanol solution according to the material-liquid ratio of 1.
Example 2
An anti-aging plant extract composition comprises the following extracts of raw materials in parts by weight: 5 parts of magnolia liliiflora, 35 parts of rhizoma gastrodiae, 30 parts of dendrobium stem and 30 parts of millet seeds.
The antiaging plant extract composition of this example was prepared in the same manner as in example 1.
Example 3
An anti-aging plant extract composition comprises the following extracts of raw materials in parts by mass: 35 parts of magnolia liliiflora, 5 parts of rhizoma gastrodiae, 30 parts of dendrobium stem and 30 parts of millet seeds.
The preparation method of the anti-aging plant extract composition comprises the following steps: weighing the raw materials of magnolia liliiflora, rhizoma gastrodiae, dendrobium nobile and millet seeds according to the proportion, crushing the raw materials into 100 meshes by a crusher, mixing the raw materials, adding the mixture into 95% ethanol solution according to the material-liquid ratio of 1:5, heating and extracting the mixture for 2 hours at 70 ℃, extracting the mixture for 3 times, filtering and collecting the extracting solution to obtain the anti-aging plant extract composition.
Example 4
An anti-aging plant extract composition comprises the following extracts of raw materials in parts by mass: 35 parts of magnolia liliiflora, 35 parts of rhizoma gastrodiae root, 5 parts of dendrobium stem and 25 parts of millet seed.
The preparation method of the anti-aging plant extract composition comprises the following steps: weighing the raw materials of magnolia liliiflora, rhizoma gastrodiae, dendrobium nobile and millet seeds according to the proportion, crushing the mixture into 200 meshes by a crusher, mixing the powder and the crushed mixture, adding the mixture into water according to the material-liquid ratio of 1 to 30, heating and extracting the mixture for 3 hours at the temperature of 80 ℃, extracting the mixture for 3 times, filtering the mixture, and collecting the extracting solution to obtain the anti-aging plant extract composition.
Example 5
An anti-aging plant extract composition comprises the following extracts of raw materials in parts by weight: 35 parts of magnolia liliiflora, 35 parts of rhizoma gastrodiae root, 25 parts of dendrobium stem and 5 parts of millet seed.
The preparation method of the anti-aging plant extract composition comprises the following steps:
weighing raw materials of magnolia liliiflora according to a ratio, crushing the magnolia liliiflora into 150 meshes by using a crusher, adding the crushed magnolia liliiflora into 75% ethanol solution according to the material-liquid ratio of 1 to 15, heating and extracting the mixture at 80 ℃ for 1.5h for 3 times, filtering the mixture, and collecting extract to obtain an magnolia liliiflora extract;
weighing rhizoma Gastrodiae according to a certain proportion, pulverizing into 150 mesh with a pulverizer, adding into 75% ethanol solution according to a material-to-liquid ratio of 1;
weighing the raw material dendrobium stem according to the proportion, crushing the dendrobium stem into 150 meshes by using a crusher, adding the dendrobium stem into 75% ethanol solution according to the material-liquid ratio of 1 to 15, heating and extracting the dendrobium stem for 1.5h at 80 ℃, extracting the dendrobium stem for 3 times, filtering, and collecting the extracting solution to obtain the dendrobium stem extract;
weighing raw material millet seeds according to a ratio, crushing the seeds into 150 meshes by using a crusher, adding the seeds into 75% ethanol solution according to a material-liquid ratio of 1;
mixing the magnolia liliiflora extract, the gastrodia elata root extract, the dendrobium nobile extract and the millet seed extract to obtain the anti-aging plant extract composition.
Comparative example 1 (differing from example 1 in that a single component is used)
A plant extract comprises the following extracts of raw materials in parts by mass: 30 parts of magnolia liliflora. The preparation method is the same as that of example 1.
Comparative example 2 (different from example 1 in that a single component was used)
A plant extract comprises the following extracts of raw materials in parts by mass: and 30 parts of rhizoma gastrodiae. The preparation method is the same as that of example 1.
Comparative example 3 (differing from example 1 in that a single component is used)
A plant extract comprises the following extracts of raw materials in parts by mass: 20 parts of dendrobium stem. The preparation method is the same as example 1.
Comparative example 4 (different from example 1 in that a single component was used)
A plant extract comprises the following raw material extracts in parts by mass: and 20 parts of millet seeds. The preparation method is the same as that of example 1.
Comparative example 5 (different from example 1 in that no millet seeds were contained)
A plant extract composition comprises the following extracts of raw materials in parts by weight: 30 parts of magnolia liliflora, 30 parts of gastrodia elata root and 20 parts of dendrobium stem. The preparation method is the same as that of example 1.
Comparative example 6 (different from example 1 in that it does not contain stem of Dendrobium nobile Lindl.)
A plant extract composition comprises the following extracts of raw materials in parts by weight: 30 parts of magnolia liliiflora, 30 parts of rhizoma gastrodiae root and 20 parts of millet seed. The preparation method is the same as that of example 1.
Comparative example 7 (different from example 1 in that no rhizoma Gastrodiae is contained)
A plant extract composition comprises the following extracts of raw materials in parts by weight: 30 parts of magnolia liliiflora, 20 parts of dendrobium stem and 20 parts of millet seeds. The preparation method is the same as that of example 1.
Comparative example 8 (different from example 1 in that it does not contain Magnolia liliflora)
A plant extract composition comprises the following extracts of raw materials in parts by weight: 30 parts of rhizoma gastrodiae, 20 parts of dendrobium stem and 20 parts of millet seeds. The preparation method is the same as that of example 1.
Comparative example 9 (different from example 1 in that magnolia liliiflora is replaced with ginseng root having similar efficacy)
A plant extract composition comprises the following extracts of raw materials in parts by weight: 30 parts of ginseng root, 30 parts of gastrodia elata root, 20 parts of dendrobium stem and 20 parts of millet seed. The preparation method is the same as that of example 1.
Comparative example 10 (different from example 1 in that the root of Gastrodia elata Blume was replaced with green tea having similar effects)
A plant extract composition comprises the following extracts of raw materials in parts by weight: 30 parts of magnolia liliiflora, 30 parts of green tea, 20 parts of dendrobium stem and 20 parts of millet seeds. The preparation method is the same as that of example 1.
Comparative example 11 (different from example 1 in that dendrobium stem was replaced with pansy having similar efficacy)
A plant extract composition comprises the following extracts of raw materials in parts by weight: 30 parts of magnolia liliiflora, 30 parts of rhizoma gastrodiae root, 20 parts of pansy and 20 parts of millet seed. The preparation method is the same as that of example 1.
Comparative example 12 (difference from example 1 in that millet seeds were replaced with soybeans having similar efficacy)
A plant extract composition comprises the following extracts of raw materials in parts by weight: 30 parts of magnolia liliiflora, 30 parts of rhizoma gastrodiae root, 20 parts of dendrobium stem and 20 parts of soybean. The preparation method is the same as that of example 1.
Effect test of plant extract composition
Experimental example 1 Metalloproteinase (MMP-1) inhibition experiment
One of the main causes of skin aging is the alteration of the structure of the dermis layer. Due to the action of some external factors, matrix Metalloproteinases (MMPs) in the body are activated, which can cause excessive degradation of collagen and elastin which support the skin structure in the dermis of the skin, thus causing the skin to have aging symptoms such as shrinkage, inelasticity, and the like. MMP-1 is also called collagenase-1, and can degrade collagen types I, II, III, VII and X, gelatin and proteoglycan. The anti-aging effects of the samples of examples 1-5 and comparative examples 1-12 were evaluated by testing the inhibition rates of these samples for MMP-1.
Fibroblasts were seeded into 12-well cell culture plates, each well containing 0.75X 10 5 Cells were cultured and starved in serum-free medium for 24 hours. Starved cultured cells were washed with PBS and treated with uv light (40 mJ). Then, the test sample was added to the cells 2 times within 48 hours. MMP-1 isolated from the medium was measured using a kit (BIOTRAK, RPN 2610). The strength of the anti-aging effect is evaluated by calculating the expression inhibition rate of MMP-1. The calculation formula is as follows:
inhibition rate = (a-B)/a × 100%;
in the formula:
a: the expression level of MMP-1 after ultraviolet irradiation without adding a test sample;
b: adding test sample and ultraviolet irradiating to express MMP-1.
The results are shown in Table 1.
TABLE 1 inhibition of MMP-1 expression by different test samples
| Test sample | MMP-1 inhibitory Rate (%) |
| Example 1 | 93.24 |
| Example 2 | 74.75 |
| Example 3 | 77.69 |
| Example 4 | 68.42 |
| Example 5 | 65.73 |
| Comparative example 1 | 26.61 |
| Comparative example 2 | 37.46 |
| Comparative example 3 | 1.35 |
| Comparative example 4 | 13.82 |
| Comparative example 5 | 45.38 |
| Comparative example 6 | 47.21 |
| Comparative example 7 | 46.49 |
| Comparative example 8 | 48.36 |
| Comparative example 9 | 36.23 |
| Comparative example 10 | 42.71 |
| Comparative example 11 | 25.28 |
| Comparative example 12 | 43.46 |
As can be seen from Table 1, the anti-aging plant extract composition of the present invention has a good inhibitory effect on metalloprotease (MMP-1), and the four components have a good synergistic effect. From experimental data, the compositions of examples 1-5 all have significantly greater inhibitory activity against metalloproteases (MMP-1) than the compositions of comparative examples 1-4, i.e., the effect of the individual components, wherein the metalloprotease inhibitory rate of the sample of example 1 reaches 93.24%. Through experimental results, the dendrobium nobile stem extract and the millet seed extract have weak inhibition effect on the metalloproteinase (MMP-1), but after the dendrobium nobile stem extract and the millet seed extract are respectively compounded with the other three components, the inhibition rate of the metalloproteinase (MMP-1) of the whole composition can be obviously improved, which shows that the four components of the composition have a synergistic relationship, and if one component is lacked, the effect is greatly reduced. In addition, as can be seen from the experimental results of comparative examples 9 to 12, even though any one of the components in the composition of the present invention is replaced with the other component having similar effects, the metalloproteinase (MMP-1) inhibition ratio is much lower than that of the compositions of examples 1 to 5, further demonstrating that there is a synergistic relationship among the components in the anti-aging plant extract composition of the present invention, which is none at all.
Experimental example 2 promotion of collagen type III Synthesis
Human fibroblasts cultured in a DMEM medium containing 10-percent FBS were seeded in a 24-well plate, and after cell attachment, medium exchange was performed using a DMEM medium containing 0.25% FBS and 250. Mu.M magnesium ascorbyl phosphate, and the test samples of examples 1-5 and comparative examples 1-12 were added, respectively. After three days of culture, the culture medium supernatant was recovered, centrifuged, and the type III collagen in the resulting supernatant was measured.
The evaluation method of the ability of type III collagen to biosynthesize in cells was carried out by measuring the content of a terminal peptide (Procollagen type III-peptide: abbreviated as PIII P) of type III collagen secreted in the supernatant using a "RIA-gnost PIII P (PIII P) measurement kit". The anti-aging effects of examples 1 to 3 and comparative examples 1 to 12 were evaluated by the promotion rate of type III collagen synthesis. The calculation formula is as follows:
promotion rate = (a-B)/a × 100%;
in the formula:
a: the amount of type III collagen produced after addition of the test sample;
b: amount of type III collagen produced without addition of test sample.
The results of the experiment are shown in table 2.
TABLE 2 promotion of collagen type III Synthesis by different test samples
The experimental results in table 2 show that the anti-aging plant extract composition of the present invention has very strong effect of promoting the generation of type iii collagen, and the highest promotion rate of the synthesis of type iii collagen in example 1 reaches 97.35%, and the effect is significantly better than that of the samples with single component in comparative examples 1 to 4 and the plant extract compositions with one component lacking in comparative examples 5 to 8. Comparative examples 9 to 12, in which a component of the composition of example 1 was equally replaced with other plant components having similar effects, have much lower collagen type III production promotion rates than those of examples 1 to 5 from the experimental results, indicating that the anti-aging plant extract composition of the present invention has a very good synergistic effect, and the effect is greatly reduced in the absence of a component.
Experimental example 3 measurement of radical scavenging ability
3.1 determination of superoxide anion radical scavenging ability
Taking 4.5mL of Tris-HCl buffer solution with the pH value of 8.2 and the concentration of 0.05mol/L, preheating the solution in a water bath at 25 ℃ for 20min, respectively adding 1mL of the plant extraction composition of the examples 1-5 and the comparative examples 1-12, then adding 0.4mL of the pyrogallol solution with the concentration of 25mmol/L, uniformly mixing, reacting in a water bath at 25 ℃ for 5min, and adding 1.0mL of HCl with the concentration of 8mol/L to terminate the reaction.
Absorbance values were measured at 299nm with Tris-HCl buffer as a reference. The blank was replaced with 1mL of distilled water, and the superoxide anion radical scavenging rate (D1) was calculated as follows.
D1(％)＝[1-(A 2 /A 1 )]×100％；
In the formula: a. The 1 Absorbance values for the blank; a. The 2 Is the absorbance value of the sample.
3.2 measurement of hydroxyl radical scavenging ability
3mL of FeSO with the concentration of 2mmol/L are sequentially added into a 25mL colorimetric tube 4 3mL of H with a concentration of 1mmol/L 2 O 2 Shaking, adding salicylic acid 3mL with concentration of 6mmol/L, shaking, heating in 37 deg.C water bath for 15min, taking out, and measuring its absorbance A 0 . Then 100 mg. L of the solution was added -1 The plant extract compositions of examples 1-5 and comparative examples 1-12 were shaken, heated in a water bath for 15min, and then taken out to measure the absorbance A x . According to the method, another colorimetric tube is taken, the above liquids are repeatedly and sequentially added, no salicylic acid is added, the mixture is shaken up and heated in water bath for 15min, and the value A is measured x0 . The hydroxyl radical clearance (D2) was calculated by the following formula.
D2(％)＝A 0 -(A x -A x0 )/A 0 ×100％；
In the formula: a. The 0 The absorbance value of the blank control system; a. The x Adding the absorbance value of a sample system to be detected; a. The x0 Without adding a color-developing agent H 2 O 2 Absorbance value of solution background.
The results of the measurement of the scavenging ability for two kinds of radicals are shown in Table 3.
TABLE 3 clearance of two free radicals
As can be seen from Table 3, the compositions obtained in examples 1-5 all have better radical scavenging ability, the scavenging rate of superoxide anion radicals reaches 95.43% at most, and the scavenging rate of hydroxyl radicals reaches 92.61% at most. The radical scavenging effect of the compositions obtained in examples 1-5 was much stronger than that of comparative examples 1-4 using the single component alone, and also much stronger than that of comparative examples 5-8 combining any three components together. The compositions obtained in comparative examples 9 to 12 are also much less effective in scavenging free radicals than the samples of examples 1 to 3 of the present invention because one of the components in the composition of the present invention is replaced with a component having similar efficacy. Therefore, the anti-aging plant extract composition has a synergistic effect, the four components are absent, and the composition has strong free radical scavenging capability and can effectively improve skin aging problems such as skin darkness, yellowing and the like caused by free radicals.
Application example 1
The plant extract composition obtained in example 1 of the present invention was used to prepare a skin cream, and the formulation components of the skin cream are shown in table 4.
TABLE 4 skin cream formulations
The skin cream is prepared according to a conventional preparation method.
Comparative application example 1 (differing from application example 1 only in that the plant extract composition of example 1 was not contained, and other components and contents and preparation method were maintained).
Experimental example 4 human skin moisture content test
The skin creams prepared in application example 1 and comparative application example 1 were used as a comparative subject, and the capacitance of the stratum corneum before and after use was measured by a Corneometer CM825 to measure the change in the water content of the stratum corneum.
The subjects were 30 in total, 20 women and 10 men, aged 20-40 years, and the average age was 28 years. Cleaning forearm of the testee, sitting at a set humidity for 30min, and selecting 3 × 3cm of forearm of the testee 2 The left arm of the square experimental area of (1) was used as a test area of the skin cream of application example 1 and comparative application example 1, the symmetric area of the right arm was used as a blank control, the moisture content of each experimental part was detected by Corneometer CM825, and the repetition was repeated 5 times to obtain an average value for recording. The results are shown in table 5, and the skin hydration rate is calculated as:
table 5 average skin hydration (%) -of 30 test volunteers at different time intervals
As can be seen from the experimental data in Table 5, the moisturizing effect of the sample added with the plant extract composition of example 1 is much higher than that of the sample without the plant extract composition of example 1, which indicates that the anti-aging plant extract composition provided by the invention has better moisturizing effect and can well improve the dry and water-deficient phenomenon of skin.
Experimental example 5 skin elasticity test
The skin elasticity was analyzed using a skin elasticity tester MPA580 (CourageKhazaka, germany) which sucks the skin into a specific probe under negative pressure, the depth of the sucked skin was determined by means of a non-contact optical test system, and MPA software was used to analyze the elastic properties of the skin. The ratio of the amount of skin springback in the absence of negative pressure to the maximum amount of skin stretching in the presence of negative pressure was used to evaluate skin elasticity, and the closer the ratio was to 1, the better the skin elasticity was. In this experiment, the changes in skin elasticity of the subjects before and after the skin cream of application example 1 and comparative application example 1 were compared, and 30 subjects including 20 women and 10 men were used in total, and the average age was 28 years.
The specific method comprises the following steps: the subjects were each daily smeared with a fixed amount of the sample on the whole face for 28 days, followed by visits at D0 (initial), D14 (day 14) and D28 (day 28) which required thorough skin cleansing, and elasticity tests after sitting still in a constant temperature and humidity environment for 30min, the results of which are shown in fig. 1.
As can be seen from the results of the skin elasticity test data in fig. 1, the elasticity index of the skin of the subject was improved by more than 50% after 4 weeks using the skin cream of application example 1 (containing 2% of the plant extract composition of example 1); the subjects who used the product of comparative application example 1 had no significant change in the elasticity index of the skin, which further illustrates that the anti-aging plant extract composition provided by the present invention has good effects in improving the elasticity of the skin, improving the firmness of the skin, reducing wrinkles, etc.
While the preferred embodiments of the present invention have been illustrated and described, it will be understood by those skilled in the art that the present invention is not limited to the details of the embodiments shown and described, but is capable of numerous equivalents and substitutions without departing from the spirit of the invention as set forth in the claims appended hereto.
Claims (7)
1. A plant extract composition comprising extracts of magnolia liliiflora, gastrodia elata root, dendrobium stem and millet seeds;
the weight parts of the raw materials are as follows:
5-35 parts of magnolia liliiflora, 5-35 parts of rhizoma gastrodiae, 5-30 parts of dendrobium stem and 5-30 parts of millet seeds;
the preparation method of the plant extract composition comprises the following steps:
pulverizing Magnolia liliflora, radix Gastrodiae, herba Dendrobii, and semen Panici Miliacei, mixing, adding into solvent, heating for extraction, filtering, and collecting extractive solution to obtain plant extract composition; the solvent is water or ethanol solution with the mass concentration of 75-95%.
2. The plant extract composition as claimed in claim 1, wherein the mass parts of the raw materials are as follows:
5-30 parts of magnolia liliiflora, 5-30 parts of rhizoma gastrodiae, 5-25 parts of dendrobium stem and 5-25 parts of millet seeds.
3. Use of the plant extract composition of claim 1 or 2 for the preparation of a cosmetic.
4. A cosmetic comprising the plant extract composition of claim 1 or 2 and an adjuvant.
5. The cosmetic according to claim 4, wherein the plant extract composition is present in an amount of 0.01 to 20% by weight of the cosmetic.
6. The cosmetic according to claim 5, wherein the plant extract composition is present in an amount of 0.01 to 2% by mass of the cosmetic.
7. The cosmetic according to claim 4, wherein the auxiliary material comprises at least one of an emulsifier, a thickener, a fat, an antioxidant, and a preservative.
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| CN202210595625.1A CN114886813B (en) | 2022-05-27 | 2022-05-27 | Anti-aging plant extract composition and preparation method and application thereof |
| PCT/CN2022/135072 WO2023226346A1 (en) | 2022-05-27 | 2022-11-29 | Anti-aging plant extract composition, preparation method therefor and use thereof |
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| CN115350133A (en) * | 2022-08-29 | 2022-11-18 | 广东轻工职业技术学院 | Preparation and application of skin-whitening and soothing nano-structure lipid carrier |
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| CN103623055A (en) * | 2012-08-22 | 2014-03-12 | 龙海波 | Applications of root and peel extract products of magnolia delavayi, southern magnolia, magnolia liliiflora and michelia alba |
| CN106236676B (en) * | 2016-10-23 | 2021-04-06 | 广州市孝真生物科技有限公司 | Plant composition with anti-aging effect and application thereof |
| CN106860118A (en) * | 2017-03-25 | 2017-06-20 | 广州市聚吉科绿色化学共性技术研究院有限公司 | A kind of anti-aging face cream |
| CN107349156A (en) * | 2017-08-28 | 2017-11-17 | 广州环亚化妆品科技有限公司 | A kind of plant composition and its preparation method and application |
| KR102126469B1 (en) * | 2017-09-04 | 2020-06-24 | 주식회사 엘지생활건강 | Cosmetic Compositions comprising extract of Dendrobium candidum flower extract |
| CN108096143A (en) * | 2017-12-28 | 2018-06-01 | 广州蜜妆生物科技有限公司 | A kind of toner containing active ingredient of Chinese herbs and yeast |
| CN108078854B (en) * | 2018-01-10 | 2019-01-08 | 壹田（广州）生活健康用品有限公司 | Hand frost and preparation method thereof is repaired in a kind of moisturizing |
| CN111228197A (en) * | 2020-04-01 | 2020-06-05 | 广州今盛美精细化工有限公司 | Dendrobium officinale extract and application thereof in preparation of cosmetics |
| AU2020101156A4 (en) * | 2020-06-26 | 2020-07-30 | Guangzhou Boteng Trading Ltd. | A Dendrobium Extract, A Method For Preparing It And Its Application |
| CN112315864A (en) * | 2020-11-17 | 2021-02-05 | 广东省粤企产业创新研究院 | Whitening and brightening composition and preparation method thereof |
| CN113855615A (en) * | 2021-11-23 | 2021-12-31 | 贵州华波日化有限公司 | Environment-friendly skin-moistening shower gel containing gastrodia elata extract and preparation method of shower gel |
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