KR102213723B1 - Cosmetic composition for improving anti-wrinkle containing the extract mori radicis cortex, Lindera obtusiloba and Lespedeza cuneata - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving wrinkles containing extracts of Sangbaekpi, Ginger tree and Yagwanmun as an active ingredient. After extracting Sangbaekpi, Ginger tree and Yagwanmun under optimal conditions and measuring the yield, antioxidant, whitening, and wrinkle improvement of the extract The activity was measured, and a cosmetic composition was developed in the form of a cream and a serum to confirm the wrinkle improvement effect. In the present invention, the cosmetic composition prepared by extracting Sangbaekpi, Gingerwood and Yagwanmun under optimal conditions not only increased wrinkle improvement activity, It has an excellent effect that can maximize the utilization of ginger trees and night gates.

Description

Cosmetic composition for improving anti-wrinkle containing the extract mori radicis cortex, Lindera obtusiloba and Lespedeza cuneata}

The present invention relates to a cosmetic composition for improving wrinkles containing the extracts of Sangbaekpi, Ginger tree and Yagwanmun as active ingredients.

Sangbaekpi is a root fatigue of the Moraceae plant, a deciduous tree belonging to the Moraceae family, and has been known to have antipyretic, anticonvulsant, anti-allergic, and anti-inflammatory effects as well as promoting diuresis (Kim, AK et al., 1995, Liver protective effects of Mori Radicis Cortex in isolated hepatocytes, J. Korea Soc. Hygienic Sciences, 1, 16-20). In particular, as it is known that oxyresveratrol such as rutin can be used as a raw material for functional cosmetics, the effect of inhibiting tyrosinase activity of Sangbaek skin (Jung, SW et al., 1995, Screening of tyrosinase inhibitor from plants, Kor. J. Food Sci. Technol ., 27, 891-896), antimicrobial and anti-acne antibacterial effect (Park, UY et al., 1995, Purification of antimicrobial substance for the extract from the root bark of Morus alba ., J. Fd Hyg-Safety, 10, 225-230) and hair growth effects on alopecia (Lee, HS et al., 2000, Hair growth effects of Mori Cortex Radicis mixture on the hair of rat, Korean J. Seric. Sci., 42, 83-86) Has been verified.

The ginger tree (Lindera obtusiloba Blume) is a plant of the Lauraceae family that grows in the shade of the forests or valleys of the Korean peninsula, Japan, and China, and is said to be a ginger tree because it smells of ginger when it is broken. The stem bark, leaves, and branches of the ginger tree contain capric acid, lauric acid, myristic acid, linderic acid, oleic acid, and linoleic acid. ), and it is known to treat bruises and hematogenous pain (Bang, CY et al., 2008, Antioxidant activities and whitening effect from lindera obtusiloba BL Extract, Yakhak Hoeji, 52, 355).

Yagwanmun (夜關門, Lespedeza cuneata G. Don) is a perennial plant belonging to the genus Lespedeza of the leguminosae. It has been used to treat the back. The physiologically active substances of the night gate include pinitol, flavoid, and phenol, and are reported to have free radicals scavenging and antioxidant activity (Lee, HJ et al., 2011, Antibacterial and antioxidative activity of Lespedeza cuneata G. Don extracts, Korean J. Microbiol Biotechnol, 39, 63-69).

Skin aging can be divided into intrinsic aging, which causes a decrease in the physiological function and structural changes of the skin as we age, and photo aging, which is an exogenous aging that causes clinical or histological changes in skin due to prolonged UV exposure. (Seo, JY et al., 2001, Skin aging from phenotype to mechanisms, Kor J Invest Dermatol, 8, 187-194) In the skin of endogenous aging, fine wrinkles increase and a soft touch is shown, whereas photoaging is a deep wrinkle on the skin. , Skin relaxation, capillary dilatation, and roughness (Seite, S. et al., 2006, Elastin change during chronological and photo-ageing: the important role of lysozyme, J Eur Acad Dermatol Venereol, 20, 980-987 ).

In the 1990s, Botox, which was used to treat muscle neurological diseases, was known to be effective in improving wrinkles around the eyes, and it began to be used for cosmetic purposes. When Botox is injected into the muscle, it blocks the neurotransmitters that move the muscle, causing muscle paralysis and atrophy of the muscle, thereby improving facial wrinkles. However, since it is not permanent, there is a disadvantage that the drug should be injected every 4 to 6 months. In the case of Botox in a liquid formulation using albumin, there are side effects that cause hypersensitivity reactions or drug resistance to the patient. Therefore, in recent years, there are increasing cases of developing functional cosmetics using extracts of various plants and herbal medicinal herbs that are harmless to the human body and do not cause hypersensitivity reactions to the skin, but it is a reality that it is rare that the level of efficacy for consumers is greatly satisfied.

As a cosmetic composition using herbal medicines, it contains extracts of plant mixtures consisting of licorice, horseback riding, hawthorn, black sesame seeds, mushrooms, angelica, mosquito, white peony, gosam, and gold that have skin protection effect against ultraviolet rays and whitening and anti-wrinkle effects. The cosmetic composition and its manufacturing method have been disclosed in Korean Patent No. 10-0966835, and a composition for skin whitening and wrinkle improvement including a ginger tree leaf extract has been disclosed in Korean Patent No. 10-0120922. In addition, a mixed herbal extract for skin whitening and anti-aging obtained from Pungryuaceae and Yagwanmun, and a cosmetic composition containing the same have been disclosed in Korean Patent Registration No. 10-1114499. However, none of the above documents discloses a cosmetic composition for improving wrinkles in which the extracts of Sangbaekpi, Ginger tree and Yagwanmun are mixed.

Accordingly, an object of the present invention is to provide an extract of Sangbaekpi, Ginger tree and Yagwanmun having anti-wrinkle activity.

Another object of the present invention is to provide a cosmetic composition for improving wrinkles containing the Sangbaekpi, Ginger tree and Yagwanmun extract.

The above object of the present invention is to prepare an extract by confirming the optimal extraction conditions for Sangbaekpi, Ginger tree and Yagwanmun; Measuring the yield of the extract; Measuring the antioxidant activity of the extract; Measuring whitening activity; Measuring the anti-wrinkle activity; Preparing a cosmetic composition of the present invention; It was achieved through the step of measuring the wrinkle improvement effect of the cosmetic of the present invention.

The present invention has an excellent effect of maximizing the use of Sangbaekpi, Ginger tree, and Yagwanmun, as well as increasing the anti-wrinkle activity by extracting Sangbaekpi, Ginger tree and Yagwanmun under optimal conditions and using it in cosmetics.

1 is a diagram of a manufacturing process of a cream composition for wrinkle improvement functional cosmetic containing the extract of Sangbaekpi, Ginger tree and Yagwanmun as active ingredients according to the present invention.
Figure 2 is a manufacturing process diagram of a serum composition for wrinkle-improving functional cosmetics containing the extract of Sangbaekpi, Ginger tree and Yagwanmun according to the present invention as active ingredients.

In the present invention, the extract may be in the form of a concentrate and a powder, and most preferably, it is prepared in the form of a lyophilized powder.

The composition of the present invention may be prepared in a dosage form such as powder, gel, ointment, cream, or liquid and solid, and general ingredients formulated in the composition for external application for skin, such as antibiotics, binders, disintegrants, and diluents. , A lubricant, a stabilizer, a preservative, or a flavoring agent, etc., can be used in appropriate combination.

In addition, the composition according to the present invention may be used according to a conventional method of use, and the number of times of use thereof may be varied according to a user's condition.

The samples used when preparing the composition for the functional cosmetic composition for improving wrinkles of the present invention were manufactured by Daeshin Techgen.

The most preferable extraction conditions in the present invention were temperature 80° C., 6.6 hours, and ethanol concentration 38 to 68%.

Hereinafter, the present invention will be described in more detail according to examples. However, the following examples are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.

<Example 1> Sangbaekpi, Ginger tree and Yagwanmun extract preparation

The Sangbaek Peel, Ginger Tree, and Yagwanmun used in the present invention were purchased and used domestically.In order to check the optimal extraction conditions, the Sangbaekpi, Ginger Tree, and Yagwanmun were freeze-dried and then extracted to 5 g of pulverized freeze-dried ground Extracted by adding 100 times the solvent (w/w). As for the extraction conditions, the three variables of extraction temperature, extraction time, and ethanol concentration were encoded in two steps of -1 and 1, and a response surface analysis method was used, as shown in [Table 1]. The extract was filtered using Whatman No. 2 filter paper to remove impurities, and the filtered solution was concentrated under reduced pressure using a vacuum concentrator or freeze-dried, and then frozen and used as a sample for analysis.

The results of the experiment were analyzed using SAS 9.1 and Design-Expert 8.0 program, and the analysis was conducted for significance through F-test on the model. In addition, for the experimental results that are not explained by the quadratic regression equation, a one-way ANOVA was performed using SAS to determine the difference between the experimental groups. For optimization, numerical optimization and graphical optimization of mixture components were selected, and the points at that time were predicted and shown.

Invention Response Surface Analysis Experiment Design Design number Extraction temperature (℃) Extraction time (h) Ethanol concentration (%) One -1(70) -1(5) -1(30) 2 -1(70) -1(5) +1(70) 3 -1(70) +1(7) -1(30) 4 -1(70) +1(7) +1(70) 5 +1(85) -1(5) -1(30) 6 +1(85) -1(5) +1(70) 7 +1(85) +1(7) -1(30) 8 +1(85) +1(7) +1(70)

<Experimental Example 1> Yield measurement of the present invention Sangbaekpi, Ginger tree and Yagwanmun extract

The extract prepared according to Example 1 of the present invention was lyophilized to measure the extraction yield.

As a result of the experiment, as shown in [Table 2], the preferred extraction conditions were 80° C., 6.6 hours, and 38-68% ethanol concentration.

Yield of extracts of Sangbaekpi, Ginger tree, and Yagwanmun number Sangbaek skin (% solids) Ginger tree (% solids) Night gate (% solids) One 0.34 0.20 0.30 2 0.32 0.20 0.33 3 0.36 0.21 0.33 4 0.38 0.22 0.32 5 0.40 0.33 0.46 6 0.41 0.32 0.47 7 0.43 0.37 0.53 8 0.45 0.36 0.57

<Experimental Example 2> Measurement of the antioxidant activity of the extract of the present invention Sangbaekpi, Ginger tree, Yagwanmun and the mixture of the three substances

Antioxidant activity was measured by adding 0.1 mL of the extract to 2 mL of a methanol solution containing 0.1 mM DPPH, vortexing, and reacting at room temperature for 10 minutes, and then measuring the absorbance at 517 nm to measure the decrease in absorbance due to reduction of DPPH.

At this time, the DPPH radical scavenging ability was calculated and measured according to [Equation 1].

A _sample : Absorbance (515 nm) of sample-added well

A _blank : absorbance (515 nm) of a well added with distilled water

Experimental results [Table 3] As shown in the showed the present invention extracts a high DPPH inhibitory activity IC ₅₀ value of Mori Cortex has to 442.80 μg / mL, ginger tree IC ₅₀ was 247.23 μg / mL, IC ₅₀ of yagwanmun is 520.45 The IC ₅₀ of the mixture of the three substances, μg/mL, showed a high DPPH inhibitory activity of 221.19 μg/mL.

DPPH inhibitory activity of the present invention Sangbaekpi, Ginger tree, Yagwanmun extract and a mixture of three sample Inhibition rate (IC ₅₀ , μg/mL) Sangbaek blood 442.80 Ginger tree 247.23 Night gate 520.45 Mixture (1:1:1) 221.19

<Experimental Example 3> Measurement of whitening activity of the present invention Sangbaekpi, Ginger tree, Yagwanmun extract, and a mixture of the three substances

The whitening activity was measured after adding 500 μL of 0.1 M sodium phosphate buffer (pH 6.8), 450 μL of distilled water, 50 μL of the present invention extract, and 50 μL of tyrosinase in sequence, and then 2.5 mM L-3,4 dihydroxyphenylalanin (L-DOPA) as a substrate. ) 500 μL was mixed, reacted at 37° C. for 1 minute, and absorbance was measured at 475 nm.

At this time, the tyrosinase inhibitory activity was calculated and measured according to [Equation 2].

As a result of the experiment, as shown in [Table 4], the IC ₅₀ value of the Sangbaekpi extract of the present invention exhibited a tyrosinase inhibitory activity of 412.70 μg/mL, and the IC ₅₀ of the ginger tree was 625.52 μg/mL, 512.40 μg/mL, and 512.40 μg/mL for the night gate. The IC ₅₀ of the mixture of the three substances showed a high tyrosinase inhibitory activity of 433.46 μg/mL.

Inhibitory activity of tyrosinase of the present invention Sangbaekpi, Ginger tree, Yagwanmun extract and three mixture sample Inhibition rate (IC ₅₀ , μg/mL) Sangbaek blood 412.70 Ginger tree 625.52 Night gate 512.40 Mixture (1:1:1) 433.46

<Experimental Example 4> Measurement of anti-wrinkle activity of the present invention Sangbaekpi, Ginger tree, Yagwanmun extract and a mixture of the three substances

Wrinkle improvement activity was measured by confirming the elastase inhibitory activity, and 60 μL of the extract of the present invention and 100 μl of a 0.6 units/ml elastase enzyme (Sigma, USA) solution were reacted at 37° C. for 30 minutes. Then, 40 μl of 4 mM sucAla3-PNA solution (Sigma, United States of America) was added and reacted at 37° C. for 30 minutes, and absorbance was measured at 405 nm using a spectrophotometer. At this time, the elastase inhibitory activity was calculated and measured according to [Equation 3].

As a result of the experiment, as shown in [Table 5], the IC ₅₀ value of the extract of the present invention showed an elastase inhibitory activity of 212.70 μg/mL, the IC ₅₀ of the ginger tree was 325.52 μg/mL, and the night gate 228.40 μg/mL mL, the IC ₅₀ of the mixture of the three substances showed high elastase inhibitory activity of 182.46 μg/mL.

Inhibitory activity of elastase inhibitory activity of the present invention Sangbaekpi, Ginger tree, Yagwanmun extract and mixture sample Inhibition rate (IC ₅₀ , μg/mL) Sangbaek blood 212.70 Ginger tree 325.52 Night gate 228.40 Mixture (1:1:1) 182.46

<Example 2> Preparation of the cosmetic composition of the present invention

(1) Wrinkle-improving functional cosmetic cream composition containing extracts of Sangbaekpi, Ginger tree and Yagwanmun as active ingredients.

The cream formulation of the cosmetic composition of the present invention was prepared in a basic form as a general purpose product. As shown in [Table 6] of the present invention, purified water of blending group A is 28 to 32% by weight, 1,3-BUTYLENE GLYCOL 12 to 14% by weight, GLYCERINE 2 to 3% by weight, METHYL PARABEN 0.25 to 0.5% by weight , Hyaluronic Acid (1%) 0.5 ~ 1% by weight, blended at 65°C and blended group B of Emersol 7036 2 ~ 3% by weight, CRODAMOL OP 6 ~ 7% by weight, Macadamia Nut Oil 3 ~ 4% by weight, ODO (TCG ) 3 ~ 4% by weight was blended at 80°C, mixed with Group A, and then first emulsified at 3500rpm and 65°C. In the emulsion, 20 to 21% by weight of purified water of group C thickener, 6 to 7% by weight of Carbopol 940 (2%) were added and mixed at 3500rpm and 70°C, and then 0.5 to 1% by weight of the extract of the present invention of the compound D group, 0.15 to 0.3% by weight of Sangbaekpi extract and 1.75 to 2.35% by weight of ginger tree extract were added, followed by mixing and cooling at 3500 rpm at 60°C to prepare a cosmetic composition of the present invention cream formulation.

Wrinkle-improving functional cosmetic cream composition containing extracts of Sangbaekpi, Ginger tree and Yagwanmun as active ingredients of the present invention Combination matter Mixing ratio (% by weight) A Purified water 28 to 32 1,3-BUTYLENE GLYCOL 12 to 14 GLYCERINE 2 to 3 METHYL PARABEN 0.25 ~ 0.5 Hyaluronic Acid (1%) 0.5 to 1 B Emersol 7036 2 to 3 CRODAMOL OP 6 to 7 Macadamia Nut Oil 3 to 4 O.D.O (TCG) 3 to 4 C Purified water 20 to 21 Carbopol 940 (2%) 6 to 7 D Night Gate Extract 0.5 to 1 Sangbaekpi extract 0.15 ~ 0.3 Ginger tree extract 1.75 ~ 2.35

(2) Wrinkle-improving serum composition containing extracts of Sangbaekpi, Ginger tree and Yagwanmun as active ingredients

In the cosmetic composition of the present invention, the serum formulation was prepared in a form in which the effect of improving wrinkles was enhanced because a large amount of extracts of Sangbaekpi, Ginger tree and Yagwanmun of the present invention were contained. As shown in the present invention [Table 7], the purified water of blending group A is 16 to 18% by weight, glycerin 6 to 8% by weight, hydroviton 24 2 to 3% by weight, and konjac mannan 4 to 5.4% by weight compared to the total weight, and the above In the mixture, 10 to 12% by weight of Hyaluronic acid and 20 to 24% by weight of carbopol 940 were mixed. To the above formulation, 9 to 10% by weight of the Yagwanmun extract and 8.4 to 9.3% by weight of Sangbaekpi extract and 10.4 to 11.3% by weight of Ginger tree extract were mixed and degassed for 30 minutes and then filtered to prepare a cosmetic composition of the serum formulation of the present invention. .

Serum composition for wrinkle-improving functional cosmetics containing extracts of Sangbaekpi, Ginger tree, and Yagwanmun as active ingredients of the present invention Combination matter Mixing ratio (% by weight) A Purified water 16 to 18 glycerin 6 to 8 hydroviton 24 2 to 3 konjac mannan 4 ~ 5.4 B Hyaluronic acid 10 to 12 carbopol 940 20 to 24 C Night Gate Extract 9-10 Sangbaekpi extract 8.4 ~ 9.3 Ginger tree extract 10.4 ~ 11.3

<Experimental Example 5> Measurement of the anti-wrinkle effect of the cosmetic composition of the present invention

In order to confirm the effect of improving skin wrinkles on the serum and cream of the cosmetic composition according to the embodiment of the present invention for 20 adult males and females, the extracted extract was applied to the skin and the depth of wrinkles was applied using a Visiometer. And the area were measured. It was applied to the skin twice a day for 1 month, and after 1 month, it was measured using a vizometer.

As a result of the experiment, as shown in [Table 8], as a result of testing the effect of improving skin wrinkles of the extract by the method for preparing a cosmetic composition for skin whitening and wrinkle improvement according to an embodiment of the present invention, on average, 20 test subjects Among them, 16 (80%) showed the effect of improving skin wrinkles.

Anti-wrinkle effect of the cosmetic composition of the present invention Distinct effect (persons) Effective (persons) No difference (persons) Invention Cream 5 12 3 Serum of the Invention 8 11 One

The present invention is a very useful invention in the cosmetic industry because it has an excellent effect of providing an extract derived from Sangbaekpi, Ginger tree, and Yagwanmun as well as providing a cosmetic composition for improving wrinkles containing the extract as an active ingredient.

Claims (6)

A cosmetic composition comprising an extract obtained by extracting each or a weight ratio of 1:1:1 mixture of Sangbaekpi, Ginger tree, and Yagwanmun plants at a temperature of 70 to 85°C at 30 to 75% ethanol concentration for 5 to 7 hours.
A wrinkle-improving functional cosmetic composition comprising the extract of claim 1 as an active ingredient.
Purified water, 1,3-BUTYLENE GLYCOL, GLYCERINE, METHYL PARABEN, and Hyaluronic Acid (1%) were mixed, and Emersol 7036, CRODAMOL OP, Macadamia Nut Oil, ODO (TCG) were added to the mixture and mixed. After that, purified water, Carbopol 940 (2%) is added to the emulsion, mixed, and blended and cooled by adding the Yagwanmun extract, Sangbaekpi extract, and Ginger tree extract of claim 1 to prepare a wrinkle-improving functional cosmetic cream. Method of making the composition.
The wrinkle-improving functional cosmetic cream composition prepared by the method of claim 3 contains 48 to 54% by weight of purified water, 12 to 14% by weight of 1,3-BUTYLENE GLYCOL, 2 to 3% by weight of GLYCERINE, 0.25 to 0.5 of METHYL PARABEN. Wt%, Hyaluronic Acid (1%) 0.5 to 1 wt%, Emersol 7036 2 to 3 wt%, CRODAMOL OP 6 to 7 wt%, Macadamia Nut Oil 3 to 4 wt%, ODO (TCG) 3 to 4 wt%, Carbopol 940 (2%) 6 to 7% by weight, 0.5 to 1% by weight of Yagwanmun extract, 0.15 to 0.3% by weight of Sangbaekpi extract, and 1.75 to 2.35% by weight of Ginger tree extract. Composition.
Purified water, glycerin, hydroviton 24, and konjac mannan are mixed, and hyaluronic acid and carbopol 940 are added to the mixture, followed by mixing and degassing for 30 minutes. A method for producing a serum composition for wrinkle-improving functional cosmetic, characterized in that it is manufactured as.
The wrinkle-improving functional serum cosmetics of claim 5 are purified water 16 to 18% by weight, glycerin 6 to 8% by weight, hydroviton 24 2 to 3% by weight, konjac mannan 4 to 5.4% by weight, hyaluronic acid 10 to 12% by weight, Carbopol 940 20 to 24% by weight, Yagwanmun extract 9 to 10% by weight, Sangbaekpi extract 8.4 to 9.3% by weight, Ginger tree extract 10.4 to 11.3% by weight A serum composition for wrinkle-improving functional cosmetic, characterized in that it is prepared by mixing.

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