CN115350133A - Preparation and application of skin-whitening and soothing nano-structure lipid carrier - Google Patents

Abstract

The invention discloses preparation and application of a nano-structure lipid carrier for whitening and relieving skin. The nano-structured lipid carrier comprises the following components in parts by weight: 10 to 30 parts of whitening active matter, 10 to 20 parts of soothing active matter, 25 to 36 parts of grease, 1 to 4 parts of emulsifier and the balance of deionized water. According to the invention, through optimizing a preparation formula and a process, whitening agents with different mechanisms are stably wrapped in the nano-structure lipid carrier, so that the whitening and relieving nano-structure lipid carrier with strong efficacy, high stability and high safety is obtained. The active matter with the whitening and relieving effects is wrapped in the nano-structure lipid carrier, and can be further applied to various skin care cosmetics to realize the effects of fading color spots and whitening. Meanwhile, the skin can be relieved, and the irritation can be reduced. The skin whitening agent enables consumers to whiten skin, reduces irritation to the skin and enhances the penetration of active substances.

Description

Preparation and application of a whitening and soothing nanostructured lipid carrier

technical field

The invention relates to a preparation method of a whitening and soothing nanostructured lipid carrier, and also relates to the application of the whitening and soothing nanostructured lipid carrier as a main active ingredient of cosmetics, belonging to the technical field of daily skin care products.

Background technique

Everyone has a desire for beauty, and fair skin is one of the common demands of oriental women. The pursuit of "whitening" mainly inhibits melanin from four aspects: one is to hinder the production of melanin. Melanin is a biological pigment secreted by melanocytes. It is oxidized to dopa by tyrosine through the action of tyrosinase. Dopa is converted into indole quinone through oxidation, decarboxylation and other reactions, and finally indole quinone is polymerized into melanin. By using cosmetics with whitening functional ingredients, melanin production can be slowed down from the source, such as arbutin, etc.; the second is to inhibit tyrosinase activity, the signaling pathway that regulates melanin formation is related to microphage-related transcription factor (MITF), and down-regulating MITF activity can Reduce the expression of related enzymes, thereby inhibiting the formation of melanin, such as resveratrol, narcissus bulb alcohol extract, etc.; the third is to inhibit the transfer of melanin, preventing the transfer of melanin to the upper keratinocytes, such as nicotinamide, etc.; the fourth is to promote Metabolize, remove melanin keratinocytes, restore fair skin, such as various acids, etc.

Contents of the invention

The technical problem to be solved by the present invention is to provide a method for preparing a whitening and soothing nanostructured lipid carrier, the steps of which at least include:

Step 1: Preparation of Purple Magnolia Leaf Extract

After cleaning the fresh purple magnolia leaves, drain and dry them; dry them until the surface is free of moisture, which is less than 70% of the original weight, and then use a grinder to grind them into purple magnolia leaf powder;

The dried magnolia leaf powder was ultrasonically crushed and extracted with absolute ethanol for 3 times, the extracts were combined, and the organic solvent was distilled off under reduced pressure to obtain the magnolia leaf extract;

Step 2: Preparation of Aloe Vera Extract

Rinse the aloe vera repeatedly, dry the surface and remove its side thorns, peel off the epidermis from the transparent gel surface, and wash off the yellow juice on the gel surface with water;

Cut the aloe vera into small pieces, stir it into a homogenate with a tissue mixer, centrifuge the homogenate at 12000r/min, remove the precipitate after 15min, and obtain the aloe vera gel juice;

Concentrate the aloe vera gel juice in a rotary evaporator to 35% of the original volume in vacuum at 60°C, then add 10 times the volume of 95% ethanol, and cool down to 4°C for alcohol precipitation for 24 hours;

The obtained Aloe Vera mixed sediment was centrifuged at a speed of 5000r/min for 15min, the precipitate was collected, and freeze-dried to obtain the Aloe Vera crude extract;

10g of Aloe vera crude extract was dissolved in 1000mL of deionized water to prepare a 1.0% aqueous solution;

Slowly add ammonium sulfate solution to the above solution at room temperature and stir rapidly until the mass fraction of ammonium sulfate in the Aloe vera crude extract solution reaches 40%, and place the mixed product at 4°C for 24 hours;

Then the above product was centrifuged at 5000r/min for 15min, the precipitate produced by centrifugation was redissolved in water and dialyzed until there was no ammonium sulfate residue, and the Aloe vera extract was obtained after freeze-drying;

Step 3: Material Preparation

According to the total weight of 100 parts, the material is:

10-30 parts of whitening active ingredients, 10-20 parts of soothing active ingredients, 25-36 parts of oil phase components, 1-4 parts of emulsifier, and the balance is deionized water;

Wherein the purple magnolia leaf extract and the aloe vera extract prepared by the method of steps 1 and 2 of the soothing active ingredient;

Step 4: Prepare the Oil Phase

The whitening active ingredient, the soothing active ingredient and the components of the oil phase in step 3 are heated and stirred to dissolve, and kept at 60-80°C to obtain the oil phase;

Step 5: Prepare the Water Phase

The emulsifier and deionized water in step 3 are heated and stirred to dissolve, and kept at 60-80°C to obtain the water phase;

Step 6: Prepare Colostrum

Slowly add the water phase obtained in step 5 into the oil phase obtained in step 4 under the action of a shear force of 10,000-16,000 r/min, and then homogenize for 2-10 minutes to obtain colostrum;

Step 7: Preparation of Nanostructured Lipid Carriers

The colostrum obtained in step 6 is homogenized 2 to 5 times by a high-pressure homogenizer under the action of a pressure of 500-800 bar, and rapidly cooled to room temperature to obtain a whitening and soothing nano-structured lipid carrier.

Specifically, the particle size of the whitening and soothing nanostructured lipid carrier is between 200nm and 600nm.

Specifically, the whitening active ingredient is 5-15 parts of α-arbutin and 5-15 parts of resveratrol.

Specifically, the components of the oil phase are 10-16 parts of shea butter, 5-11 parts of monoglyceride, 10-15 parts of lauryl alcohol, 1-6 parts of caprylic and capric triglycerides, 2-8 parts of Isooctyl Palmitate.

Specifically, the emulsifier is: 1-4 parts of Span 83 and 2-3 parts of Tween 80.

A whitening emulsion prepared by using the whitening and soothing nano-structured lipid carrier, the whitening emulsion includes the following components by mass percentage:

A kind of whitening emulsion that utilizes the described whitening and soothing nanostructure lipid carrier to participate in the preparation, and its preparation method comprises the following steps:

Stir and mix the components of Phase A, Phase B, and Phase C separately and set aside; first, heat Phase A and Phase B to 80°C, dissolve and mix evenly, add Phase A to Phase B, and homogenize for 5 minutes. Stir at a rotation speed of 8000rpm/min, then cool to 50°C, add phase C and homogenize for 2min, age for 24h and pack.

A kind of whitening facial mask liquid prepared by utilizing the nano-structured lipid carrier for whitening and soothing, comprising the following components by mass percentage:

A kind of whitening facial mask liquid that utilizes the nanostructure lipid carrier of described whitening and soothing to participate in the preparation, and its preparation method comprises the following steps:

Stir and mix the components of Phase A, Phase B and Phase C separately and set aside; weigh Phases A and B in sequence, heat Phases A and B to 80°C, dissolve until there are no particles, and stir evenly at a speed of 1000rpm/min , add phase B to phase A, homogenize for 5 minutes, stir and cool down to 50°C, add phase C, homogenize at a low speed of 800rpm/min for 2min, age for 24h and pack.

Compared with the prior art, the present invention has the following beneficial effects:

The present invention utilizes consumers' needs for whitening cosmetics and combines skin sensitivity to prepare a nanostructured lipid carrier with both whitening and soothing effects, allowing consumers to reduce the troubles caused by sensitive skin while loving beauty . The product prepared by the invention has both whitening and soothing effects by encapsulating the active substance in the nanostructured lipid carrier, so that the carrier can not only have the whitening effect, but also play a soothing effect and reduce skin irritation.

Description of drawings

Figure 1 is a schematic diagram of the effect of the whitening and soothing nanostructured lipid carrier (sample 1) and the raw material solution (sample 2) of the nanostructured lipid carrier not coated with active substances on the safety of zebrafish embryos at corresponding concentrations (P<0.01* *; P<0.001***);

Figure 2 is a schematic diagram of the effect of whitening and soothing nanostructured lipid carriers on the whitening effect of zebrafish embryos at corresponding concentrations (P<0.01**; P<0.001***; P<0.0001****);

Figure 3 is a schematic diagram of the effect of different concentrations of whitening and soothing nanostructured lipid carriers on the soothing effect of zebrafish embryos (P<0.0001****).

Detailed ways

The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Apparently, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments provided by the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" etc. The indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, or in a specific orientation. construction and operation, therefore, should not be construed as limiting the invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and should not be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that unless otherwise specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection. Connected, or integrally connected; it can be mechanically connected or electrically connected; it can be directly connected or indirectly connected through an intermediary, and it can be the internal communication of two components. Those of ordinary skill in the art can understand the specific meanings of the above terms in the present invention in specific situations.

Embodiment: the preparation of purple magnolia leaf extract

Step 1: Processing the Purple Magnolia Leaves

After washing the fresh purple magnolia leaves, drain and dry them. After drying until the surface is free of moisture and less than 70% of the original weight, it is ground into purple magnolia leaf powder with a grinder.

Step 2: Extraction of Purple Magnolia Leaves

The dried magnolia leaf powder was ultrasonically crushed and extracted three times with absolute ethanol, the extracts were combined, and the organic solvent was distilled off under reduced pressure to obtain the magnolia leaf extract.

Example: Preparation of Aloe Vera Extract

Step 1: Processing Aloe Vera

Rinse the aloe vera repeatedly, dry the surface and remove its side thorns, peel off the epidermis from the transparent gel surface, and wash off the yellow juice on the gel surface with water.

Step 2: Extraction of Aloe Vera

Cut the aloe vera into small pieces, stir it into a homogenate with a tissue mixer, centrifuge the homogenate at 12000r/min, remove the precipitate after 15min, and obtain the aloe vera gel juice.

The aloe vera gel juice was vacuum-concentrated to 35% of the original volume at 60°C in a rotary evaporator, then 10 times the volume of 95% ethanol was added, and the temperature was lowered to 4°C for alcohol precipitation for 24 hours.

The obtained Aloe vera mixed sediment was centrifuged at a speed of 5000r/min for 15min, the precipitate was collected, and freeze-dried to obtain a crude Aloe vera extract.

Step 3: Separation and Purification of Aloe Vera Extract

A 1.0% aqueous solution was prepared by dissolving 10 g of Aloe vera crude extract in 1000 mL of deionized water.

Slowly add ammonium sulfate solution to the above solution at room temperature and stir the ammonium sulfate solution rapidly until the mass fraction of ammonium sulfate in the Aloe vera crude extract solution reaches 40%, then place the mixed product in a 4°C environment Leave it for 24h.

Then the above product was centrifuged at 5000r/min for 15min, the centrifuged precipitate was redissolved in water and dialyzed until no ammonium sulfate remained, and the Aloe vera extract was obtained after freeze-drying.

The magnolia leaf extract and Aloe vera extract prepared by the above method were used in the following examples.

Example 1

The steps for preparing the magnolia leaf extract and the aloe vera extract are not repeated.

Step 3: Material Preparation

According to the total weight of 100 parts, the material is:

Whitening active ingredients include: 8 parts of α-arbutin, 6 parts of resveratrol.

Soothing active ingredients include: 6 parts Magnolia Leaf Extract, 8 parts Aloe Vera Extract.

The components of the oil phase include solid fats and liquid fats.

Solid fats include: 11 parts of shea butter, 7 parts of monoglyceride, and 10 parts of lauryl alcohol.

Liquid oils include: 2 parts caprylic capric triglyceride, 4 parts isooctyl palmitate.

The emulsifier is: 2 parts of Span 83, 1 part of Tween 80,

Then add 35 parts of deionized water to make up the parts by weight.

Step 4: Prepare the Oil Phase

The whitening active ingredient, the soothing active ingredient and the oil phase components in step 3 are heated and stirred to dissolve, and kept at 60°C to obtain the oil phase;

Step 5: Prepare the Water Phase

Heat and stir the emulsifier and deionized water in step 3 to dissolve, and keep warm at 60°C to obtain the water phase;

Step 6: Prepare Colostrum

Slowly add the water phase obtained in step 5 to the oil phase obtained in step 4 under the shear force of 12000r/min, and then homogenize for 4 minutes to obtain colostrum;

Step 7: Preparation of Nanostructured Lipid Carriers

The colostrum obtained in step 6 is homogenized 4 times by a high-pressure homogenizer under a pressure of 500 bar, and rapidly cooled to room temperature to obtain a whitening and soothing nano-structured lipid carrier.

The particle size of the whitening and soothing nano-structured lipid carrier is detected to be between 200nm and 600nm.

Example 2

The steps for preparing the magnolia leaf extract and the aloe vera extract are not repeated.

Step 3: Material Preparation

According to the total weight of 100 parts, the material is:

Whitening active ingredients include: 8 parts of α-arbutin, 6 parts of resveratrol.

Soothing active ingredients include: 7 parts Magnolia Leaf Extract, 8 parts Aloe Vera Extract.

The components of the oil phase include solid fats and liquid fats.

Solid fats include: 11 parts of shea butter, 7 parts of monoglyceride, and 10 parts of lauryl alcohol.

Liquid oils include: 2 parts caprylic capric triglyceride, 4 parts isooctyl palmitate.

The emulsifier is: 1 part of Span 83, 2 parts of Tween 80,

Then add 34 parts of deionized water to make up the parts by weight.

Step 4: Prepare the Oil Phase

Heat and stir the whitening active ingredient, soothing active ingredient and components of the oil phase in step 3 to dissolve, and keep warm at 65°C to obtain the oil phase;

Step 5: Prepare the Water Phase

Heat and stir the emulsifier and deionized water in step 3 to dissolve, keep warm at 65°C to obtain the water phase;

Step 6: Prepare Colostrum

Slowly add the water phase obtained in step 5 into the oil phase obtained in step 4 under the shear force of 14000r/min, and then homogenize for 5 minutes to obtain colostrum;

Step 7: Preparation of Nanostructured Lipid Carriers

The colostrum obtained in step 6 is homogenized 5 times by a high-pressure homogenizer under the action of a pressure of 500 bar, and rapidly cooled to room temperature to obtain a whitening and soothing nano-structured lipid carrier.

The particle size of the whitening and soothing nano-structured lipid carrier is detected to be between 200nm and 600nm.

Example 3

The steps for preparing the magnolia leaf extract and the aloe vera extract are not repeated.

Step 3: Material Preparation

According to the total weight of 100 parts, the material is:

Whitening active ingredients include: 10 parts of α-arbutin, 10 parts of resveratrol.

Soothing active ingredients include: 10 parts Magnolia Leaf Extract, 10 parts Aloe Vera Extract.

The components of the oil phase include solid fats and liquid fats.

Solid fats include: 12 parts shea butter, 5 parts monoglyceride, 11 parts lauryl alcohol.

Liquid oils include: 4 parts caprylic capric triglyceride, 4 parts isooctyl palmitate.

The emulsifier is: 2 parts of Span 83, 2 parts of Tween 80,

Then add 20 parts of deionized water to make up the parts by weight.

Step 4: Prepare the Oil Phase

The whitening active ingredient, the soothing active ingredient and the components of the oil phase in step 3 are heated and stirred to dissolve, and kept at 70°C to obtain the oil phase;

Step 5: Prepare the Water Phase

Heat and stir the emulsifier and deionized water in step 3 to dissolve, keep warm at 70°C to obtain the water phase;

Step 6: Prepare Colostrum

Slowly add the water phase obtained in step 5 to the oil phase obtained in step 4 under the shear force of 10000r/min, and then homogenize for 5 minutes to obtain colostrum;

Step 7: Preparation of Nanostructured Lipid Carriers

The colostrum obtained in step 6 is homogenized 4 times by a high-pressure homogenizer under the action of a pressure of 600 bar, and rapidly cooled to room temperature to obtain a whitening and soothing nano-structured lipid carrier.

The particle size of the whitening and soothing nano-structured lipid carrier is detected to be between 200nm and 600nm.

Example 4

The steps for preparing the magnolia leaf extract and the aloe vera extract are not repeated.

Step 3: Material Preparation

According to the total weight of 100 parts, the material is:

Whitening active ingredients include: 8 parts of α-arbutin, 6 parts of resveratrol.

Soothing active ingredients include: 7 parts Magnolia Leaf Extract, 8 parts Aloe Vera Extract.

The components of the oil phase include solid fats and liquid fats.

Solid fats include: 12 parts shea butter, 7 parts monoglyceride, 11 parts lauryl alcohol.

Liquid oils include: 2 parts caprylic capric triglyceride, 4 parts isooctyl palmitate.

The emulsifier is: 2 parts of Span 83, 1 part of Tween 80,

Then add 32 parts of deionized water to make up the parts by weight.

Step 4: Prepare the Oil Phase

Heat and stir the whitening active ingredient, soothing active ingredient and components of the oil phase in step 3 to dissolve, and keep warm at 75°C to obtain the oil phase;

Step 5: Prepare the Water Phase

Heat and stir the emulsifier and deionized water in step 3 to dissolve, keep warm at 75°C to obtain the water phase;

Step 6: Prepare Colostrum

Slowly add the water phase obtained in step 5 to the oil phase obtained in step 4 under the shear force of 12000r/min, and then homogenize for 4 minutes to obtain colostrum;

Step 7: Preparation of Nanostructured Lipid Carriers

The colostrum obtained in step 6 was homogenized four times by a high-pressure homogenizer at a pressure of 700 bar, and cooled rapidly to room temperature to obtain a whitening and soothing nanostructured lipid carrier.

The particle size of the whitening and soothing nano-structured lipid carrier is detected to be between 200nm and 600nm.

Example 5

The steps for preparing the magnolia leaf extract and the aloe vera extract are not repeated.

Step 3: Material Preparation

According to the total weight of 100 parts, the material is:

Whitening active ingredients include: 10 parts of α-arbutin, 10 parts of resveratrol.

Soothing active ingredients include: 7 parts Magnolia Leaf Extract, 12 parts Aloe Vera Extract.

The components of the oil phase include solid fats and liquid fats.

Solid fats include: 12 parts shea butter, 7 parts monoglyceride, 11 parts lauryl alcohol.

Liquid oils include: 2 parts caprylic capric triglyceride, 4 parts isooctyl palmitate.

The emulsifier is: 2 parts of Span 83, 2 parts of Tween 80,

Then add 21 parts of deionized water to make up the parts by weight.

Step 4: Prepare the Oil Phase

Heat and stir the whitening active ingredient, soothing active ingredient and components of the oil phase in step 3 to dissolve, and keep warm at 65°C to obtain the oil phase;

Step 5: Prepare the Water Phase

Heat and stir the emulsifier and deionized water in step 3 to dissolve, keep warm at 65°C to obtain the water phase;

Step 6: Prepare Colostrum

Slowly add the water phase obtained in step 5 to the oil phase obtained in step 4 under the shear force of 14000r/min, and then homogenize for 6 minutes to obtain colostrum;

Step 7: Preparation of Nanostructured Lipid Carriers

The colostrum obtained in step 6 is homogenized 5 times by a high-pressure homogenizer under the action of a pressure of 600 bar, and rapidly cooled to room temperature to obtain a whitening and soothing nano-structured lipid carrier.

The particle size of the whitening and soothing nano-structured lipid carrier is detected to be between 200nm and 600nm.

Example 6

Examination of the effect of whitening and soothing nanostructured lipid carriers on the safety of zebrafish embryos.

Zebrafish embryo safety evaluation principle: Expose zebrafish embryos at the 128-cell stage to 96hpf in the dilution of the test substance, then observe and record the number of dead fish embryos with the characteristics of condensation, no heartbeat or tail detachment under the microscope, and calculate the number of embryos mortality rate.

Operation steps: In the spawning box, fish are paired with a male-to-female ratio of 1:2 to spawn. In the morning, the light is turned on to stimulate the male and female fish to mate. After 30 minutes of mating, the embryos are collected and rinsed with breeding water.

The 6hpf embryos were observed under a stereomicroscope, and embryos with normal development were screened for subsequent experiments. Place the embryos in a 24-well plate, 20 embryos per well, set up three repetitions, remove the standard dilution water in the well plate without harming the embryos, and quickly add 1mL of a certain concentration of whitening and soothing nanostructures to each well Lipid carrier solution (sample 1), the whitening and soothing nanostructured lipid carrier raw material product is directly mixed with the same concentration of components to form the same concentration, uncoated active nanostructure Lipid carrier solution (sample 2) and blank solution.

Incubate in the dark in a constant temperature incubator at 28.5°C, observe the development of the embryos every day, remove the dead embryos in time, and replace 100% of the drug solution, so as not to affect the development of the embryos. After exposure to 96hpf, the death number of embryos was observed and recorded, and the mortality rate was calculated. The result is shown in Figure 1. (P<0.01**; P<0.001***).

Embodiment 7:

Examination of the effect of whitening and soothing nanostructured lipid carrier on the whitening efficacy of zebrafish embryos

Efficacy evaluation principle of zebrafish embryo whitening: place 24hpf zebrafish embryos in diluents of different concentrations of test substances to 48hpf, and observe the changes in the distribution of melanin granules according to the deposition and distribution of melanin on the embryo body surface.

Operation steps: In the spawning box, fish are paired with a male-to-female ratio of 1:2 to spawn. In the morning, the light is turned on to stimulate the male and female fish to mate. After 30 minutes of mating, the embryos are collected and rinsed with breeding water.

Observe under a stereomicroscope to screen embryos that develop normally and can be used for subsequent experiments. Select 24hpf zebrafish embryos with normal development, place them in a 24-well plate, 20 embryos per well, set up three replicates, remove the standard dilution water in the well plate without harming the embryos, and quickly add 1mL of a certain concentration to each well Whitening and soothing nanostructured lipid carrier solution and blank solution.

Incubate in a constant temperature incubator at 28.5°C in the dark. After incubation for 24hpf, at least 10 zebrafish were randomly selected from zebrafish with normal phenotype and behavior, fixed with 3% methylcellulose, observed and photographed under a fluorescent microscope, Use image analysis software to analyze the zebrafish pictures to calculate the melanin content of the zebrafish. The result is shown in Figure 2. (P<0.01**; P<0.001***; P<0.0001****). The results show that the whitening and soothing nano-structured lipid carrier of the present invention can obviously inhibit the melanin on the body surface of zebrafish, and has a remarkable whitening effect.

Experimental example 8

Testing the soothing efficacy of whitening and soothing nanostructured lipid carriers on zebrafish embryos

The principle of evaluating the soothing efficacy of zebrafish embryos: place 24hpf zebrafish embryos in diluents of different concentrations of the test substance, and after incubation for a period of time, observe the rolling of the embryos to evaluate the soothing effect of the test substance.

Operation steps: In the spawning box, fish are paired with a male-to-female ratio of 1:2 to spawn. In the morning, the light is turned on to stimulate the male and female fish to mate. After 30 minutes of mating, the embryos are collected and rinsed with breeding water. Observe under a stereomicroscope to screen embryos that develop normally and can be used for subsequent experiments.

Select 24hpf zebrafish embryos with normal development, place them in a 96-well plate, 5 embryos per well, set up 3 groups of repetitions, remove the standard dilution water in the well plate without harming the embryos, and add different concentrations of whitening and soothing nano Dilution of structured lipid carrier, add buffer water to the blank control group and model control group, add modeling agent to the positive control group, incubate for one hour, remove the liquid in the well after incubation, add buffer water to the blank group, and add SDS solution to the model group , adding the solution prepared by the modeling agent to the test substance, and after incubation for 15 minutes, record the number of times each embryo rolled over within 30 seconds. The result is shown in Figure 3.

The results show that the whitening and soothing nano-structured lipid carrier of the present invention can significantly reduce the number of tumbling times of zebrafish embryos, and has a significant soothing effect.

Embodiment 9: A kind of whitening emulsion (the application of nanostructure lipid carrier that whitens and relieves)

The whitening emulsion is prepared with the whitening and soothing nano-structured lipid carrier as the key component, which includes the following components by mass percentage:

Preparation:

Stir and mix the components of Phase A, Phase B and Phase C separately and set aside for later use. First, heat A and B phases to 80°C respectively, dissolve and mix evenly, add phase A into phase B, and homogenize for 5 minutes, stir at a speed of 8000rpm/min, then cool to 50°C, add phase C and mix Homogenize for 2 minutes, pack after aging for 24 hours.

Embodiment 10: A kind of whitening mask liquid (the application of nanostructure lipid carrier that whitens and relieves)

The whitening mask liquid is prepared with the whitening and soothing nano-structured lipid carrier as the key component, which includes the following components by mass percentage:

Preparation:

Stir and mix the components of Phase A, Phase B and Phase C separately and set aside for later use. Mix phases A and B in turn, heat phases A and B to 80°C, dissolve until there are no particles, stir at a speed of 1000rpm/min, add phase B to phase A, homogenize for 5min, stir and cool down to 50°C , add phase C, homogenize at a low speed of 800rpm/min for 2min, age for 24h and pack.

Embodiment 11, human body trial evaluation experiment

1. Experimental method

Example 9: a whitening emulsion (application of whitening and soothing nanostructured lipid carrier); embodiment 10: a whitening mask liquid (application of whitening and soothing nanostructured lipid carrier) for human trial evaluation.

Select 50 volunteers aged 18-60 and divide them into two groups. The method of use is to use after cleansing every day, twice a day (respectively apply once in the morning and evening), the left face of one group uses the emulsion prepared by embodiment 9, and the left face of one group uses the facial mask liquid prepared by embodiment 10; two groups The right face of the patient uses a basic skin care product (record the specific product name and model) that he usually uses without any special effects (especially without any whitening or soothing ingredients).

The test period is two months. During the test period, no other cosmetics are used on the test site. After two months of use, the skin color, spots, dullness and skin sensitivity of the left and right faces are observed.

2. Experimental results

After two months of use, it was found that a total of 44 volunteers had used the emulsion prepared in Example 9 and the skin color of the facial mask liquid prepared in Example 10. The skin color of the left face can be better than that of the right face, and the spots and The dullness is significantly improved, and at the same time, the skin's anti-sensitivity is improved. It is considered that the effective rate is as high as 88%.

The whitening and soothing nanostructured lipid carrier prepared by the present invention can be widely used in the field of cosmetics, such as creams, lotions, essences, facial masks and other cosmetics of different formulations, or different formulations such as whitening, anti-inflammatory, anti-aging, moisturizing, etc. In functional cosmetics, it has good application value.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, rather than limiting them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: It is still possible to modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technical solutions of the various embodiments of the present invention. scope.

Claims (9)

1. A preparation method of a nano-structure lipid carrier for whitening and soothing is characterized by comprising the following steps:

step 1: preparation method of Magnolia liliiflora leaf extract

Cleaning fresh magnolia liliflora leaves, draining and drying; drying until the surface is free of moisture and less than 70% of the original weight, and grinding into powder of Magnolia liliiflora leaves with a grinder;

ultrasonically crushing and extracting dried magnolia liliiflora leaf powder with absolute ethyl alcohol for 3 times, combining extracting solutions, and distilling under reduced pressure to remove an organic solvent to obtain an magnolia liliiflora leaf extract;

and 2, step: preparation method of Aloe Barbadensis Miller extract

Repeatedly washing Aloe vera, wiping surface, removing edge thorn, peeling epidermis from transparent gel surface, and washing off yellow juice on gel surface with clear water;

cutting Aloe vera into small pieces, stirring with tissue mixer to homogenate, centrifuging homogenate for 12000r/min, and removing precipitate after 15min to obtain Aloe vera gel juice;

vacuum concentrating Aloe vera gel juice in a rotary evaporator at 60 deg.C to 35% of original volume, adding 10 times volume of 95% ethanol, cooling to 4 deg.C, and precipitating with ethanol for 24 hr;

centrifuging the obtained Aloe barbadensis Miller mixed precipitate at 5000r/min for 15min, collecting precipitate, and freeze drying to obtain Aloe barbadensis Miller crude extract;

dissolving 10g of crude extract of Aloe barbadensis Miller in 1000mL of deionized water to prepare 1.0% aqueous solution;

slowly adding ammonium sulfate solution into the above solution at room temperature, rapidly stirring the ammonium sulfate solution until the mass fraction of ammonium sulfate in the crude extract solution of Aloe Barbadensis Miller reaches 40%, and standing the mixed product at 4 deg.C for 24 hr;

centrifuging the product at 5000r/min for 15min, dissolving precipitate in water, dialyzing until no ammonium sulfate residue is left, and lyophilizing to obtain Aloe Barbadensis Miller extract;

and step 3: material preparation

The materials calculated by the total weight of 100 parts are as follows:

10-30 parts of whitening active ingredients, 10-20 parts of soothing active ingredients, 25-36 parts of components of an oil phase, 1-4 parts of an emulsifier and the balance of deionized water;

wherein the soothing active ingredient is an magnolia liliiflora leaf extract and an aloe barbadensis extract prepared by the methods of steps 1 and 2;

and 4, step 4: preparation of the oil phase

Heating, stirring and dissolving the whitening active ingredients, the soothing active ingredients and the components of the oil phase in the step 3, and keeping the temperature at 60-80 ℃ to obtain the oil phase;

and 5: preparation of the aqueous phase

Heating, stirring and dissolving the emulsifier and the deionized water in the step 3, and keeping the temperature at 60-80 ℃ to obtain a water phase;

and 6: preparation of colostrum

Slowly adding the water phase obtained in the step (5) into the oil phase obtained in the step (4) under the action of the shearing force of 10000-16000 r/min, and then homogenizing for 2-10 min to obtain primary emulsion;

and 7: preparation of nanostructured lipid Carriers

Homogenizing the colostrum obtained in the step 6 for 2-5 times under the action of 500-800 bar of pressure by a high-pressure homogenizer, and rapidly cooling to room temperature to obtain the whitening and relieving nano-structure lipid carrier.

2. The whitening-soothing nanostructured lipid carrier of claim 1, wherein:

the particle size of the nano-structured lipid carrier is between 200 and 600 nm.

3. The whitening-soothing nanostructured lipid carrier of claim 1, wherein:

the whitening active components comprise 5-15 parts of alpha-arbutin and 5-15 parts of resveratrol.

4. The whitening-soothing nanostructured lipid carrier of claim 1, wherein:

the components of the oil phase are 10-16 parts of shea butter, 5-11 parts of monoglyceride, 10-15 parts of lauryl alcohol, 1-6 parts of caprylic/capric triglyceride and 2-8 parts of isooctyl palmitate.

5. The whitening-soothing nanostructured lipid carrier of claim 1, wherein:

the emulsifier is as follows: 1-4 parts of span 83 and 2-3 parts of Tween 80.

6. The whitening emulsion of any of claims 1-5, wherein the nanostructured lipid carrier is configured to: the whitening emulsion comprises the following components in percentage by mass:

7. a whitening emulsion with the incorporation of a whitening soothing nanostructured lipid carrier according to any of claims 1 to 5, characterized in that it is prepared by a process comprising the following steps:

respectively stirring and mixing the components of the phase A, the phase B and the phase C for later use; respectively heating the phase A and the phase B to 80 ℃, dissolving and mixing uniformly, adding the phase A into the phase B, homogenizing for 5min, stirring at the rotation speed of 8000rpm/min, cooling to 50 ℃, adding the phase C, homogenizing for 2min, aging for 24h, and packaging.

8. The whitening and soothing nanostructured lipid carrier according to any of claims 1 to 5, involved in the preparation of a whitening mask solution, characterized in that: the whitening mask liquid comprises the following components in percentage by mass:

9. the whitening mask solution with the participation of the whitening and soothing nano-structured lipid carrier in the preparation of any one of claims 1 to 5, characterized in that the preparation method comprises the following steps:

respectively stirring and mixing the components of the phase A, the phase B and the phase C for later use; weighing the phase A and the phase B in sequence, heating the phase A and the phase B to 80 ℃, dissolving until no particles exist, uniformly stirring at the rotation speed of 1000rpm/min, adding the phase B into the phase A, homogenizing for 5min, stirring, cooling to 50 ℃, adding the phase C, homogenizing at the rotation speed of 800rpm/min for 2min at a low speed, aging for 24h, and packaging.

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