CN115350133A

CN115350133A - Preparation and application of skin-whitening and soothing nano-structure lipid carrier

Info

Publication number: CN115350133A
Application number: CN202211056118.7A
Authority: CN
Inventors: 郑丹阳; 朱永闯; 胡翠萍; 詹朝阳; 龚盛昭; 黄金辉; 梁世劲
Original assignee: Guangdong Industry Technical College
Current assignee: Guangdong Industry Technical College
Priority date: 2022-08-29
Filing date: 2022-08-29
Publication date: 2022-11-18

Abstract

The invention discloses preparation and application of a nano-structure lipid carrier for whitening and relieving skin. The nano-structured lipid carrier comprises the following components in parts by weight: 10 to 30 parts of whitening active matter, 10 to 20 parts of soothing active matter, 25 to 36 parts of grease, 1 to 4 parts of emulsifier and the balance of deionized water. According to the invention, through optimizing a preparation formula and a process, whitening agents with different mechanisms are stably wrapped in the nano-structure lipid carrier, so that the whitening and relieving nano-structure lipid carrier with strong efficacy, high stability and high safety is obtained. The active matter with the whitening and relieving effects is wrapped in the nano-structure lipid carrier, and can be further applied to various skin care cosmetics to realize the effects of fading color spots and whitening. Meanwhile, the skin can be relieved, and the irritation can be reduced. The skin whitening agent enables consumers to whiten skin, reduces irritation to the skin and enhances the penetration of active substances.

Description

Preparation and application of skin-whitening and soothing nano-structure lipid carrier

Technical Field

The invention relates to a preparation method of a skin whitening and relieving nano-structure lipid carrier, and also relates to an application of the skin whitening and relieving nano-structure lipid carrier as a main effective component of a cosmetic, belonging to the technical field of daily skin care products.

Background

People who love beauty are all good, and white skin is one of the popular appeal of oriental women. The pursuit of "whitening" is mainly to inhibit melanin from four aspects: one is to block melanogenesis. The melanin is a biological pigment secreted by melanocytes, which is formed by the oxidation of tyrosine to dopa under the action of tyrosinase, the oxidation and decarboxylation of dopa to indoloquinone, and finally the indoloquinone is polymerized to form the melanin. By using the whitening functional component cosmetics, the generation of melanin such as arbutin and the like can be slowed down from the source; secondly, the tyrosinase activity is inhibited, a signal path for regulating the formation of melanin is related to microphage-related transcription factor (MITF), and the expression of related enzyme can be reduced by regulating the activity of the MITF, so that the formation of melanin is inhibited, such as resveratrol and narcissus bulb alcohol extract; thirdly, the transfer of melanin is inhibited, and the transfer of the melanin to upper keratinocyte is prevented, such as nicotinamide and the like; fourthly, it can promote metabolism, remove melanin horny cells and make skin white, such as various acids.

Disclosure of Invention

The technical problem to be solved by the invention is to provide a preparation method of a nano-structure lipid carrier for whitening and soothing, which at least comprises the following steps:

step 1: preparation method of Magnolia liliiflora leaf extract

Cleaning fresh magnolia liliflora leaves, draining and drying; drying until the surface has no moisture and less than 70% of the original weight, and grinding into powder of Magnolia liliflora leaves with a grinder;

ultrasonically crushing and extracting dried magnolia liliiflora leaf powder by using absolute ethyl alcohol for 3 times, combining extracting solutions, and distilling under reduced pressure to remove an organic solvent to obtain an magnolia liliiflora leaf extract;

step 2: preparation method of Aloe Barbadensis Miller extract

Repeatedly washing Aloe vera, wiping off the surface, removing the edge pricks, peeling off the epidermis from the transparent gel surface, and washing off the yellow juice on the gel surface with clear water;

cutting Aloe vera into small pieces, stirring with tissue mixer to homogenate, centrifuging homogenate for 12000r/min, and removing precipitate after 15min to obtain Aloe vera gel juice;

concentrating Aloe vera gel juice in a rotary evaporator at 60 deg.C under vacuum to 35% of original volume, adding 10 times of 95% ethanol, cooling to 4 deg.C, precipitating with ethanol for 24 hr;

centrifuging the obtained Aloe barbadensis Miller mixed precipitate at 5000r/min for 15min, collecting precipitate, and freeze drying to obtain Aloe barbadensis Miller crude extract;

dissolving 10g of Aloe vera crude extract in 1000mL of deionized water to prepare a 1.0% aqueous solution;

slowly adding ammonium sulfate solution into the above solution at room temperature, rapidly stirring until the mass fraction of ammonium sulfate in the crude extract solution of Aloe Barbadensis Miller reaches 40%, and standing the mixed product at 4 deg.C for 24 hr;

centrifuging the product at 5000r/min for 15min, dissolving precipitate in water, dialyzing until no ammonium sulfate residue is left, and lyophilizing to obtain Aloe Barbadensis Miller extract;

and step 3: material preparation

The materials calculated by 100 parts of the total weight are as follows:

10-30 parts of whitening active ingredients, 10-20 parts of soothing active ingredients, 25-36 parts of components of an oil phase, 1-4 parts of an emulsifier and the balance of deionized water;

wherein the soothing active ingredients are the magnolia liliflora leaf extract and the aloe barbadensis extract prepared by the methods of steps 1 and 2;

and 4, step 4: preparation of the oil phase

Heating, stirring and dissolving the whitening active ingredients, the soothing active ingredients and the components of the oil phase in the step 3, and keeping the temperature at 60-80 ℃ to obtain the oil phase;

and 5: preparation of the aqueous phase

Heating, stirring and dissolving the emulsifier and the deionized water in the step 3, and keeping the temperature at 60-80 ℃ to obtain a water phase;

step 6: preparation of colostrum

Slowly adding the water phase obtained in the step (5) into the oil phase obtained in the step (4) under the action of the shearing force of 10000-16000 r/min, and then homogenizing for 2-10 min to obtain primary emulsion;

and 7: preparation of nanostructured lipid Carriers

Homogenizing the colostrum obtained in the step 6 for 2-5 times under the action of 500-800 bar of pressure by a high-pressure homogenizer, and rapidly cooling to room temperature to obtain the whitening and relieving nano-structure lipid carrier.

Specifically, the particle size of the whitening and soothing nano-structure lipid carrier is 200-600 nm.

Specifically, the whitening active ingredients comprise 5-15 parts of alpha-arbutin and 5-15 parts of resveratrol.

Specifically, the oil phase comprises 10-16 parts of shea butter, 5-11 parts of monoglyceride, 10-15 parts of lauryl alcohol, 1-6 parts of caprylic/capric triglyceride and 2-8 parts of isooctyl palmitate.

Specifically, the emulsifier is: 1-4 parts of span 83 and 2-3 parts of tween 80.

The whitening emulsion prepared by using the nano-structure lipid carrier for whitening and soothing comprises the following components in percentage by mass:

the preparation method of the whitening emulsion by using the whitening and soothing nano-structure lipid carrier comprises the following steps:

respectively stirring and mixing the components of the phase A, the phase B and the phase C for later use; heating phase A and phase B to 80 deg.C respectively, dissolving, mixing, adding phase A into phase B, homogenizing for 5min, stirring at 8000rpm/min, cooling to 50 deg.C, adding phase C, homogenizing for 2min, aging for 24 hr, and packaging.

The whitening mask liquid prepared by using the whitening and soothing nano-structure lipid carrier comprises the following components in percentage by mass:

the preparation method of the whitening mask liquid by using the whitening and soothing nano-structure lipid carrier comprises the following steps:

respectively stirring and mixing the components of the phase A, the phase B and the phase C for later use; weighing the phase A and the phase B in sequence, heating the phase A and the phase B to 80 ℃, dissolving the phase A and the phase B until no particles exist, uniformly stirring at the rotation speed of 1000rpm/min, adding the phase B into the phase A, homogenizing for 5min, stirring and cooling to 50 ℃, adding the phase C, homogenizing at the low speed of 800rpm/min for 2min, aging for 24h, and packaging.

Compared with the prior art, the invention has the following beneficial effects:

according to the invention, the nano-structure lipid carrier with whitening and relieving effects is prepared by utilizing the requirements of consumers on whitening cosmetics and combining the sensitivity of skin, so that the consumers can love beauty and simultaneously reduce the trouble caused by sensitive muscles. According to the product prepared by the invention, the active substance is wrapped in the nano-structure lipid carrier, so that the carrier has whitening and relieving effects, and can play a role in relieving the effect and reducing skin irritation at the same time of having the whitening effect.

Drawings

FIG. 1 is a graphical representation of the effect of the raw material solution (sample 2) of whitened soothing nanostructured lipid carriers (sample 1), uncoated active nanostructured lipid carriers at the corresponding concentrations on the safety of zebrafish embryos (P < 0.01;. P < 0.001;);

FIG. 2 is a graphical representation of the effect of soothing-whitening nanostructured lipid carriers on the whitening effect of zebrafish embryos at the corresponding concentrations (P < 0.01; (P < 0.001; (P < 0.0001;));

figure 3 is a graphical representation of the effect of whitening soothing nanostructured lipid carriers on soothing efficacy of zebrafish embryos at different concentrations (P <0.0001 x).

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments provided by the present invention without any creative effort belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example (b): preparation method of Magnolia liliiflora leaf extract

Step 1: treating Magnolia liliflora leaves

Cleaning fresh magnolia liliiflora leaves, draining and drying. After drying to a surface moisture free, less than 70% of the original weight, it was ground to magnolia leaf powder with a grinder.

And 2, step: extraction of magnolia liliiflora leaves

Ultrasonically crushing and extracting dried magnolia liliiflora leaf powder with absolute ethyl alcohol for 3 times, combining extracting solutions, and distilling under reduced pressure to remove an organic solvent to obtain the magnolia liliiflora leaf extract.

Example (b): preparation method of Aloe Barbadensis Miller extract

Step 1: treating Aloe vera

Repeatedly washing Aloe Barbadensis Miller, wiping surface, removing edge thorn, peeling epidermis from transparent gel surface, and washing with clear water to remove yellow juice on the surface of gel.

Step 2: extraction of Aloe barbadensis Miller

Cutting Aloe vera into small pieces, stirring with tissue blender to homogenate, centrifuging the homogenate for 12000r/min, and removing precipitate after 15min to obtain Aloe vera gel juice.

Vacuum concentrating Aloe vera gel juice in rotary evaporator at 60 deg.C to 35% of original volume, adding 10 times volume of 95% ethanol, cooling to 4 deg.C, and precipitating with ethanol for 24 hr.

Centrifuging the obtained Aloe mixed precipitate at 5000r/min for 15min, collecting precipitate, and lyophilizing to obtain Aloe crude extract.

And step 3: separation and purification of aloe vera extract

A1.0% aqueous solution was prepared by dissolving 10g of crude extract of Aloe barbadensis Miller in 1000mL of deionized water.

Slowly adding ammonium sulfate solution into the above solution at room temperature, rapidly stirring the ammonium sulfate solution until the mass fraction of ammonium sulfate in the crude extract solution reaches 40%, and standing the mixture at 4 deg.C for 24 hr.

Centrifuging the product at 5000r/min for 15min, dissolving precipitate in water, dialyzing until no ammonium sulfate residue is left, and lyophilizing to obtain Aloe Barbadensis Miller extract.

The magnolia liliiflora leaf extract and aloe barbadensis extract prepared as described above were used in the following examples.

Example 1

The steps for preparing the magnolia liliiflora leaf extract and the aloe barbadensis extract are not repeated.

And step 3: material preparation

The materials calculated by the total weight of 100 parts are as follows:

the whitening active ingredients comprise: 8 parts of alpha-arbutin and 6 parts of resveratrol.

The soothing active ingredients comprise: 6 parts of magnolia liliiflora leaf extract and 8 parts of aloe barbadensis extract.

The components of the oil phase comprise solid grease and liquid grease.

The solid oil and fat comprises: 11 parts of shea butter, 7 parts of monoglyceride and 10 parts of lauryl alcohol.

The liquid grease includes: 2 parts of caprylic capric triglyceride and 4 parts of isooctyl palmitate.

The emulsifier is as follows: 2 parts of span 83, 1 part of Tween 80,

and adding 35 parts of deionized water to make up the weight parts.

And 4, step 4: preparation of the oil phase

Heating, stirring and dissolving the whitening active ingredients, the soothing active ingredients and the components of the oil phase in the step 3, and keeping the temperature at 60 ℃ to obtain the oil phase;

and 5: preparation of the aqueous phase

Heating, stirring and dissolving the emulsifier and the deionized water in the step 3, and keeping the temperature at 60 ℃ to obtain a water phase;

step 6: preparation of colostrum

Slowly adding the water phase obtained in the step (5) into the oil phase obtained in the step (4) under the action of the shearing force of 12000r/min, and then homogenizing for 4min to obtain primary emulsion;

and 7: preparation of nanostructured lipid Carriers

And (4) homogenizing the colostrum obtained in the step (6) for 4 times under the action of 500bar pressure by a high-pressure homogenizer, and rapidly cooling to room temperature to obtain the whitening and soothing nano-structure lipid carrier.

The particle size of the nano-structure lipid carrier for detecting whitening and soothing is between 200 and 600 nm.

Example 2

And step 3: material preparation

The materials calculated by the total weight of 100 parts are as follows:

The soothing active ingredients comprise: 7 parts of magnolia liliiflora leaf extract and 8 parts of aloe barbadensis extract.

The components of the oil phase comprise solid grease and liquid grease.

The emulsifier is as follows: 1 part of span 83, 2 parts of Tween 80,

then 34 parts of deionized water is added to make up the parts by weight.

And 4, step 4: preparation of the oil phase

Heating, stirring and dissolving the whitening active ingredients, the soothing active ingredients and the components of the oil phase in the step 3, and keeping the temperature at 65 ℃ to obtain the oil phase;

and 5: preparation of the aqueous phase

Heating, stirring and dissolving the emulsifier and the deionized water in the step 3, and keeping the temperature at 65 ℃ to obtain a water phase;

step 6: preparation of colostrum

Slowly adding the water phase obtained in the step (5) into the oil phase obtained in the step (4) under the action of the shearing force of 14000r/min, and then homogenizing for 5min to obtain primary emulsion;

and 7: preparation of nanostructured lipid Carriers

And (4) homogenizing the colostrum obtained in the step (6) for 5 times under the action of 500bar pressure by a high-pressure homogenizer, and rapidly cooling to room temperature to obtain the whitening and soothing nano-structure lipid carrier.

Example 3

The steps of preparing the magnolia liliiflora leaf extract and the aloe barbadensis extract are not repeated.

And 3, step 3: material preparation

The materials calculated by the total weight of 100 parts are as follows:

the whitening active ingredients comprise: 10 parts of alpha-arbutin and 10 parts of resveratrol.

The soothing active ingredients comprise: 10 parts of magnolia liliiflora leaf extract and 10 parts of aloe barbadensis extract.

The components of the oil phase comprise solid grease and liquid grease.

The solid oil and fat comprises: 12 parts of shea butter, 5 parts of monoglyceride and 11 parts of lauryl alcohol.

The liquid grease includes: 4 parts of caprylic capric triglyceride and 4 parts of isooctyl palmitate.

The emulsifier is as follows: 2 parts of span 83, 2 parts of Tween 80,

and then 20 parts of deionized water is added to make up the parts by weight.

And 4, step 4: preparation of the oil phase

Heating, stirring and dissolving the whitening active ingredients, the soothing active ingredients and the components of the oil phase in the step 3, and keeping the temperature at 70 ℃ to obtain the oil phase;

and 5: preparation of the aqueous phase

Heating, stirring and dissolving the emulsifier and the deionized water in the step 3, and keeping the temperature at 70 ℃ to obtain a water phase;

step 6: preparation of colostrum

Slowly adding the water phase obtained in the step (5) into the oil phase obtained in the step (4) under the action of the shearing force of 10000r/min, and then homogenizing for 5min to obtain primary emulsion;

and 7: preparation of nanostructured lipid Carriers

And (4) homogenizing the colostrum obtained in the step (6) for 4 times under the action of the pressure of 600bar by a high-pressure homogenizer, and rapidly cooling to room temperature to obtain the whitening and soothing nano-structure lipid carrier.

Example 4

And step 3: material preparation

The materials calculated by the total weight of 100 parts are as follows:

The components of the oil phase comprise solid grease and liquid grease.

The solid oil comprises: 12 parts of shea butter, 7 parts of monoglyceride and 11 parts of lauryl alcohol.

The liquid grease includes: 2 parts of caprylic/capric triglyceride and 4 parts of isooctyl palmitate.

The emulsifier is: 2 parts of span 83, 1 part of Tween 80,

and adding 32 parts of deionized water to make up the parts by weight.

And 4, step 4: preparation of the oil phase

Heating, stirring and dissolving the whitening active ingredients, the soothing active ingredients and the components of the oil phase in the step 3, and keeping the temperature at 75 ℃ to obtain the oil phase;

and 5: preparation of the aqueous phase

Heating, stirring and dissolving the emulsifier and the deionized water in the step 3, and keeping the temperature at 75 ℃ to obtain a water phase;

and 6: preparation of colostrum

and 7: preparation of nanostructured lipid Carriers

Homogenizing the colostrum obtained in the step 6 for 4 times under the action of 700bar pressure by a high-pressure homogenizer, and rapidly cooling to room temperature to obtain the whitening and soothing nano-structure lipid carrier.

The particle size of the nano-structure lipid carrier for whitening and relieving is detected to be between 200 and 600 nm.

Example 5

And step 3: material preparation

The materials calculated by 100 parts of the total weight are as follows:

The soothing active ingredients comprise: 7 parts of magnolia liliiflora leaf extract and 12 parts of aloe barbadensis extract.

The components of the oil phase comprise solid grease and liquid grease.

The emulsifier is as follows: 2 parts of span 83, 2 parts of Tween 80,

then 21 parts of deionized water is added to make up the parts by weight.

And 4, step 4: preparation of the oil phase

and 5: preparation of the aqueous phase

step 6: preparation of colostrum

Slowly adding the water phase obtained in the step 5 into the oil phase obtained in the step 4 under the action of the shearing force of 14000r/min, and then homogenizing for 6min to obtain primary emulsion;

and 7: preparation of nanostructured lipid Carriers

And (4) homogenizing the colostrum obtained in the step (6) for 5 times under the action of the pressure of 600bar by a high-pressure homogenizer, and rapidly cooling to room temperature to obtain the whitening and soothing nano-structure lipid carrier.

Example 6

And detecting the influence of the whitening and soothing nanostructured lipid carrier on the safety of the zebra fish embryo.

The safety evaluation principle of zebra fish embryos comprises the following steps: 128-cell stage zebrafish embryos were exposed to 96hpf of test dilution and then observed under a microscope and the number of dead fish embryos characterized by coagulation, no heart beat or no tail detachment was recorded and embryo mortality was calculated.

The method comprises the following operation steps: the fish lay eggs according to the ratio of 1.

And (3) observing the 6hpf embryo under a stereomicroscope, and screening the embryo which can be used for subsequent experiments and has normal development. The embryos are placed in a 24-pore plate, three groups of the embryos are repeated, standard dilution water in the pore plate is removed under the condition that the embryos are not damaged, 1mL of solution (sample 1) of the whitening and soothing nanostructure lipid carrier with a certain concentration is rapidly added into each pore, and the raw material product of the whitening and soothing nanostructure lipid carrier is directly and simply stirred with the components with the same concentration without any operation to prepare the solution (sample 2) of the uncoated active substance nanostructure lipid carrier with the same concentration and a blank solution.

Incubating in 28.5 deg.C incubator in dark place, observing embryo development condition every day, removing dead embryo in time, and replacing 100% medicinal liquid to avoid influencing embryo development. After exposure to 96hpf, the number of deaths of the embryos was recorded and calculated. The results are shown in FIG. 1. (P < 0.01;. P < 0.001;).

Example 7:

detecting the influence of the nano-structure lipid carrier on the whitening effect of zebra fish embryos

The evaluation principle of the zebra fish embryo whitening efficacy is as follows: and (3) placing 24hpf zebrafish embryos in test substance diluents with different concentrations until the concentration reaches 48hpf, and observing the distribution change of melanin granules according to the deposition distribution of melanin on the surface of the embryos.

Observing under a stereoscopic microscope, and screening embryos which can be used for subsequent experiments and have normal development. Selecting a 24hpf zebra fish embryo which is normal in development, placing the 24hpf zebra fish embryo in a 24-pore plate, setting three groups of repeats, removing standard dilution water in the pore plate under the condition of not damaging the embryo, and quickly adding 1mL of a whitening and relieving nano-structure lipid carrier solution with a certain concentration and a blank solution into each pore.

Incubating in a constant temperature incubator at 28.5 ℃ in a dark place, incubating for 24hpf, randomly selecting at least 10 zebra fish from the zebra fish with normal phenotype and behavior, fixing the zebra fish by using 3% methylcellulose, observing and photographing under a fluorescence microscope, and analyzing the zebra fish picture by using image analysis software to calculate the content of melanin in the zebra fish. The results are shown in FIG. 2. (P < 0.01;. P < 0.001;. P < 0.0001;). The result shows that the whitening and soothing nano-structure lipid carrier can obviously inhibit the melanin on the body surface of the zebra fish, and has a remarkable whitening effect.

Experimental example 8

Detecting the influence of the whitening and soothing nanostructured lipid carrier on the soothing effect of the zebra fish embryo

The evaluation principle of the zebra fish embryo soothing efficacy is as follows: 24hpf zebrafish embryos are placed in test substance dilution solutions with different concentrations, and after a period of incubation, the rolling condition of the embryos is observed, so that the relieving efficacy condition of the test substances is evaluated.

The method comprises the following operation steps: the fish lay eggs according to the ratio of 1. Observing under a stereoscopic microscope, and screening embryos which can be used for subsequent experiments and have normal development.

Selecting a 24hpf zebra fish embryo which is normal in development, placing the 24hpf zebra fish embryo in a 96-well plate, setting 3 groups of repeats, removing standard dilution water in the well plate under the condition of not damaging the embryo, adding dilution liquid of a lipid carrier with a nano structure with different concentrations, whitening and relieving, adding buffer water into a blank control group and a model control group, adding a modeling agent into the positive control group, incubating for one hour, removing liquid in the well after incubation is finished, adding buffer water into the blank group, adding an SDS solution into the model group, adding a solution prepared by the modeling agent into a test object, and recording the rolling times of each embryo in 30s after incubating for 15 min. The results are shown in FIG. 3.

The result shows that the skin-whitening and soothing nano-structure lipid carrier can obviously reduce the rolling times of zebra fish embryos and has a remarkable soothing effect.

Example 9: whitening emulsion (application of nano-structured lipid carrier for whitening and relieving)

The whitening emulsion is prepared by taking a nano-structure lipid carrier for whitening and soothing as a key component, and comprises the following components in percentage by mass:

the preparation method comprises the following steps:

respectively stirring and mixing the components of the phase A, the phase B and the phase C for later use. Respectively heating the phase A and the phase B to 80 ℃, dissolving and mixing uniformly, adding the phase A into the phase B, homogenizing for 5min, stirring at the rotation speed of 8000rpm/min, cooling to 50 ℃, adding the phase C, homogenizing for 2min, aging for 24h, and packaging.

Example 10: whitening mask liquid (application of nano-structure lipid carrier for whitening and relieving)

The whitening mask liquid is prepared by taking a whitening and relieving nano-structure lipid carrier as a key component, and comprises the following components in percentage by mass:

the preparation method comprises the following steps:

respectively stirring and mixing the components of the phase A, the phase B and the phase C for later use. Weighing the phase A and the phase B in sequence, heating the phase A and the phase B to 80 ℃, dissolving the phase A and the phase B until no particles exist, uniformly stirring at the rotation speed of 1000rpm/min, adding the phase B into the phase A, homogenizing for 5min, stirring and cooling to 50 ℃, adding the phase C, homogenizing at the low speed of 800rpm/min for 2min, aging for 24h, and packaging.

Example 11 human trial evaluation experiment

1. Experimental methods

The method comprises the following steps of example 9: a whitening emulsion (application of a whitening and soothing nano-structured lipid carrier); example 10: a whitening facial mask liquid (application of nano-structure lipid carrier for whitening and relieving) is used for human trial evaluation.

50 volunteers of 18-60 years old were selected and divided into two groups. The application method comprises cleaning face twice a day (applying once in the morning and evening), applying the lotion prepared in example 9 to one group of left face, and applying the facial mask solution prepared in example 10 to one group of left face; the two groups of right faces use a basic skin care product (recording specific product name and model) which has no special efficacy (especially has no whitening or relieving component) and is used in daily life.

The experimental period is two months, the tested part is not applied with other cosmetics during the experimental period, and the skin color, spots, dark condition and skin sensitivity of the left face and the right face are observed after two months of application.

2. Results of the experiment

After two months of use, it was found that the skin color of the left face was better than that of the right face in all 44 volunteers who used the lotion prepared in example 9 and the facial mask lotion prepared in example 10, and that the spots and dullness of the left face were significantly improved and the skin sensitivity was improved. The effective rate is considered to be as high as 88%.

The whitening and soothing nano-structure lipid carrier prepared by the invention can be widely applied to the field of cosmetics, such as cream, emulsion, essence, mask and other cosmetics in different formulations, or whitening, anti-inflammatory, anti-aging, moisturizing and other cosmetics with different effects, and has good application value.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A preparation method of a nano-structure lipid carrier for whitening and soothing is characterized by comprising the following steps:

step 1: preparation method of Magnolia liliiflora leaf extract

Cleaning fresh magnolia liliflora leaves, draining and drying; drying until the surface is free of moisture and less than 70% of the original weight, and grinding into powder of Magnolia liliiflora leaves with a grinder;

ultrasonically crushing and extracting dried magnolia liliiflora leaf powder with absolute ethyl alcohol for 3 times, combining extracting solutions, and distilling under reduced pressure to remove an organic solvent to obtain an magnolia liliiflora leaf extract;

and 2, step: preparation method of Aloe Barbadensis Miller extract

Repeatedly washing Aloe vera, wiping surface, removing edge thorn, peeling epidermis from transparent gel surface, and washing off yellow juice on gel surface with clear water;

vacuum concentrating Aloe vera gel juice in a rotary evaporator at 60 deg.C to 35% of original volume, adding 10 times volume of 95% ethanol, cooling to 4 deg.C, and precipitating with ethanol for 24 hr;

dissolving 10g of crude extract of Aloe barbadensis Miller in 1000mL of deionized water to prepare 1.0% aqueous solution;

slowly adding ammonium sulfate solution into the above solution at room temperature, rapidly stirring the ammonium sulfate solution until the mass fraction of ammonium sulfate in the crude extract solution of Aloe Barbadensis Miller reaches 40%, and standing the mixed product at 4 deg.C for 24 hr;

and step 3: material preparation

The materials calculated by the total weight of 100 parts are as follows:

wherein the soothing active ingredient is an magnolia liliiflora leaf extract and an aloe barbadensis extract prepared by the methods of steps 1 and 2;

and 4, step 4: preparation of the oil phase

and 5: preparation of the aqueous phase

and 6: preparation of colostrum

and 7: preparation of nanostructured lipid Carriers

2. The whitening-soothing nanostructured lipid carrier of claim 1, wherein:

the particle size of the nano-structured lipid carrier is between 200 and 600 nm.

3. The whitening-soothing nanostructured lipid carrier of claim 1, wherein:

the whitening active components comprise 5-15 parts of alpha-arbutin and 5-15 parts of resveratrol.

4. The whitening-soothing nanostructured lipid carrier of claim 1, wherein:

the components of the oil phase are 10-16 parts of shea butter, 5-11 parts of monoglyceride, 10-15 parts of lauryl alcohol, 1-6 parts of caprylic/capric triglyceride and 2-8 parts of isooctyl palmitate.

5. The whitening-soothing nanostructured lipid carrier of claim 1, wherein:

the emulsifier is as follows: 1-4 parts of span 83 and 2-3 parts of Tween 80.

6. The whitening emulsion of any of claims 1-5, wherein the nanostructured lipid carrier is configured to: the whitening emulsion comprises the following components in percentage by mass:

7. a whitening emulsion with the incorporation of a whitening soothing nanostructured lipid carrier according to any of claims 1 to 5, characterized in that it is prepared by a process comprising the following steps:

respectively stirring and mixing the components of the phase A, the phase B and the phase C for later use; respectively heating the phase A and the phase B to 80 ℃, dissolving and mixing uniformly, adding the phase A into the phase B, homogenizing for 5min, stirring at the rotation speed of 8000rpm/min, cooling to 50 ℃, adding the phase C, homogenizing for 2min, aging for 24h, and packaging.

8. The whitening and soothing nanostructured lipid carrier according to any of claims 1 to 5, involved in the preparation of a whitening mask solution, characterized in that: the whitening mask liquid comprises the following components in percentage by mass:

9. the whitening mask solution with the participation of the whitening and soothing nano-structured lipid carrier in the preparation of any one of claims 1 to 5, characterized in that the preparation method comprises the following steps:

respectively stirring and mixing the components of the phase A, the phase B and the phase C for later use; weighing the phase A and the phase B in sequence, heating the phase A and the phase B to 80 ℃, dissolving until no particles exist, uniformly stirring at the rotation speed of 1000rpm/min, adding the phase B into the phase A, homogenizing for 5min, stirring, cooling to 50 ℃, adding the phase C, homogenizing at the rotation speed of 800rpm/min for 2min at a low speed, aging for 24h, and packaging.